D. Kohlwein

Curriculum Vitae
Univ.-Prof. Dipl.-Ing. Dr. Sepp D. Kohlwein
Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50/II, 8010 Graz, Austria;
phone: +43-316-380 5487, fax: +43-316-380 9854, email: [email protected]
Website: yeast.uni-graz.at, bioimaginggraz.at
Research Interests
Lipid metabolism and membrane assembly in yeast; yeast as a model of lipid-associated
disorders; super-resolution microscopy and non-linear imaging (CARS, SHG);
implementation of novel imaging methods to investigate membrane and organelle
structure and dynamics in yeast and mammalian cells and tissue; lipidomics.
University Education
1977
Diploma in Technical Chemistry/Biochemistry, Graz University of
Technology, Austria
1982
Ph.D. thesis in Biochemistry, Graz University of Technology, Austria
Career History
1978–2001
1985–1986
1989
1992
2001
Assistant Professor, Graz University of Technology, Austria
Postdoctoral fellow, Albert Einstein College of Medicine, Bronx, NY, USA
Postdoctoral fellow, Carnegie Mellon University, Pittsburgh, PA, USA
Habilitation in Biochemistry, Associate Professor, Graz University of
Technology, Austria
Full Professor of Biochemistry, University of Graz, Austria
Career-related Activities and Memberships
1995-2005
Deputy Chairman SFB Biomembranes (speaker H. Esterbauer), Graz,
Austria
2000-07/08
Sabbatical, University of Washington, Seattle, WA, USA
2002-2004
Head of the Institute of Molecular Biosciences, University of Graz,
Austria
2005-2014
Coordinator: Lipidomics Research Center Graz
2008/12-2009/03 Sabbatical, National University of Singapore, Singapore
since 2013
Coordinator: BioImaging Graz
since 2014
Coordinator: BioTechMed Graz – Molecular Biomedicine
Honors and Awards
1985
Schrödinger Fellowship
2010
Erzherzog-Johann Research Prize of the County of Styria
Scientific Talks
2014
2013
2011
2010
2009
FASEB Lipid Droplets, VT, USA (invited plenary talk, session chair)
Yeast Lipid Conference, Halifax, Canada (invited plenary talk)
ASBMB, Washington DC, USA (invited plenary talk)
Gordon Research Conference - Lipoproteins, Waterville Valley, NH,
USA (invited plenary talk)
Gordon Research Conference – Lipids, Waterville Valley, NH, USA
(invited plenary talk)
Peer reviewed publications (present - 2009)
1. Hofbauer HF, Schopf FH, Schleifer H, Knittelfelder OL, Pieber B, Rechberger GN, Wolinski H,
Gaspar ML, Kapp OC, Stadlmann J, Mechtler K, Zenz A, Lohner K, Tehlivets O, Henry SA,
Kohlwein SD. Regulation of Gene Expression through a Transcriptional Repressor that
Senses Acyl-Chain Length in Membrane Phospholipids. Dev Cell 2014; 29(6):729-39. doi:
10.1016/j.devcel.2014.04.025.
2. Knittelfelder OL, Weberhofer BP, Eichmann TO, Kohlwein SD, Rechberger GN. A versatile
ultra-high performance LC-MS method for lipid profiling. J Chromatogr B Analyt Technol
Biomed Life Sci 2014; 951-952:119-28. doi: 10.1016/j.jchromb.2014.01.011.
3. van Zutphen T, Todde V, de Boer R, Kreim M, Hofbauer HF, Wolinski H, Veenhuis M, van der
Klei IJ, Kohlwein SD. Lipid droplet autophagy in the yeast Saccharomyces cerevisiae. Mol Biol
Cell 2014; 25(2):290-301. doi: 10.1091/mbc.E13-08-0448.
4. Guerfal M, Claes K, Knittelfelder O, De Rycke R, Kohlwein SD, Callewaert N. Enhanced
membrane protein expression by engineering increased intracellular membrane production.
Microb Cell Fact 2013; 12:122. doi: 10.1186/1475-2859-12-122.
5. Kohlwein SD, Veenhuis M, van der Klei IJ. Lipid droplets and peroxisomes: key players in
cellular lipid homeostasis or a matter of fat--store 'em up or burn 'em down. Genetics 2013;
193(1):1-50. doi: 10.1534/genetics.112.143362.
6. Natter K, Kohlwein SD. Yeast and cancer cells - common principles in lipid metabolism.
Biochim Biophys Acta 2013; 1831(2):314-26. doi: 10.1016/j.bbalip.2012.09.003.
7. Radulovic M, Knittelfelder O, Cristobal-Sarramian A, Kolb D, Wolinski H, Kohlwein SD. The
emergence of lipid droplets in yeast: current status and experimental approaches. Curr Genet
2013; 59(4):231-42. doi: 10.1007/s00294-013-0407-9.
8. Schriefl AJ, Wolinski H, Regitnig P, Kohlwein SD, Holzapfel GA. An automated approach for
three-dimensional quantification of fibrillar structures in optically cleared soft biological tissues.
J R Soc Interface 2013; 10(80):20120760. doi: 10.1098/rsif.2012.0760.
9. Sommer G, Schwarz M, Kutschera M, Kresnik R, Regitnig P, Schriefl AJ, Wolinski H, Kohlwein
SD, Holzapfel GA. Biomechanical Properties of the Human Ventricular Myocardium. Biomed
Tech (Berl) 2013. doi: 10.1515/bmt-2013-4108.
10. Beretta M, Wolkart G, Schernthaner M, Griesberger M, Neubauer R, Schmidt K, Sacherer M,
Heinzel FR, Kohlwein SD, Mayer B. Vascular bioactivation of nitroglycerin is catalyzed by
cytosolic
aldehyde
dehydrogenase-2.
Circ
Res
2012;
110(3):385-93.
doi:
10.1161/CIRCRESAHA.111.245837.
11. Hanscho M, Ruckerbauer DE, Chauhan N, Hofbauer HF, Krahulec S, Nidetzky B, Kohlwein
SD, Zanghellini J, Natter K. Nutritional requirements of the BY series of Saccharomyces
cerevisiae strains for optimum growth. FEMS Yeast Res 2012; 12(7):796-808. doi:
10.1111/j.1567-1364.2012.00830.x.
12. Henry SA, Kohlwein SD, Carman GM. Metabolism and regulation of glycerolipids in the yeast
Saccharomyces cerevisiae. Genetics 2012; 190(2):317-49. doi: 10.1534/genetics.111.130286.
13. Mattiazzi M, Sun Y, Wolinski H, Bavdek A, Petan T, Anderluh G, Kohlwein SD, Drubin DG,
Krizaj I, Petrovic U. A neurotoxic phospholipase A2 impairs yeast amphiphysin activity and
reduces endocytosis. PLoS ONE 2012; 7(7):e40931. doi: 10.1371/journal.pone.0040931.
14. Paar M, Jungst C, Steiner NA, Magnes C, Sinner F, Kolb D, Lass A, Zimmermann R,
Zumbusch A, Kohlwein SD, Wolinski H. Remodeling of lipid droplets during lipolysis and
growth in adipocytes. J Biol Chem 2012; 287(14):11164-73. doi: 10.1074/jbc.M111.316794.
15. 14.
Schernthaner M, Reisinger B, Wolinski H, Kohlwein SD, Trantina-Yates A, Fahrner M,
Romanin C, Itani H, Stifter D, Leitinger G, Groschner K, Heitz J. Nanopatterned polymer
substrates promote endothelial proliferation by initiation of beta-catenin transcriptional
signaling. Acta Biomater 2012; 8(8):2953-62. doi: 10.1016/j.actbio.2012.04.018.
16. Wolinski H, Bredies K, Kohlwein SD. Quantitative imaging of lipid metabolism in yeast: from
4D analysis to high content screens of mutant libraries. Methods Cell Biol 2012; 108:345-65.
doi: 10.1016/B978-0-12-386487-1.00016-X.
17. Zechner R, Zimmermann R, Eichmann TO, Kohlwein SD, Haemmerle G, Lass A, Madeo F.
FAT SIGNALS--lipases and lipolysis in lipid metabolism and signaling. Cell Metab 2012;
15(3):279-91. doi: 10.1016/j.cmet.2011.12.018.
18. Aflaki E, Balenga NA, Luschnig-Schratl P, Wolinski H, Povoden S, Chandak PG, BognerStrauss JG, Eder S, Konya V, Kohlwein SD, Heinemann A, Kratky D. Impaired Rho GTPase
activation abrogates cell polarization and migration in macrophages with defective lipolysis.
Cell Mol Life Sci 2011; 68(23):3933-47. doi: 10.1007/s00018-011-0688-4.
19. Aflaki E, Radovic B, Chandak PG, Kolb D, Eisenberg T, Ring J, Fertschai I, Uellen A, Wolinski
H, Kohlwein SD, Zechner R, Levak-Frank S, Sattler W, Graier WF, Malli R, Madeo F, Kratky
D. Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in
macrophages. J Biol Chem 2011; 286(9):7418-28. doi: 10.1074/jbc.M110.175703.
20. Gaspar ML, Hofbauer HF, Kohlwein SD, Henry SA. Coordination of storage lipid synthesis and
membrane biogenesis: evidence for cross-talk between triacylglycerol metabolism and
phosphatidylinositol
synthesis.
J
Biol
Chem
2011;
286(3):1696-708.
doi:
10.1074/jbc.M110.172296.
21. Seper A, Fengler VH, Roier S, Wolinski H, Kohlwein SD, Bishop AL, Camilli A, Reidl J, Schild
S. Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm
formation. Mol Microbiol 2011; 82(4):1015-37. doi: 10.1111/j.1365-2958.2011.07867.x.
22. Wolinski H, Kolb D, Hermann S, Koning RI, Kohlwein SD. A role for seipin in lipid droplet
dynamics and inheritance in yeast. J Cell Sci 2011; 124(Pt 22):3894-904. doi:
10.1242/jcs.091454.
23. Deng Y, Almsherqi ZA, Ng MM, Kohlwein SD. Do viruses subvert cholesterol homeostasis to
induce host cubic membranes? Trends Cell Biol 2010; 20(7):371-9. doi:
10.1016/j.tcb.2010.04.001.
24. Gruber A, Cornaciu I, Lass A, Schweiger M, Poeschl M, Eder C, Kumari M, Schoiswohl G,
Wolinski H, Kohlwein SD, Zechner R, Zimmermann R, Oberer M. The N-terminal region of
comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation
of adipose triglyceride lipase. J Biol Chem 2010; 285(16):12289-98. doi:
10.1074/jbc.M109.064469.
25. Heier C, Taschler U, Rengachari S, Oberer M, Wolinski H, Natter K, Kohlwein SD, Leber R,
Zimmermann R. Identification of Yju3p as functional orthologue of mammalian monoglyceride
lipase in the yeast Saccharomyces cerevisiae. Biochim Biophys Acta 2010; 1801(9):1063-71.
doi: 10.1016/j.bbalip.2010.06.001.
26. Kohlwein SD. Triacylglycerol homeostasis: insights from yeast. J Biol Chem 2010;
285(21):15663-7. doi: 10.1074/jbc.R110.118356.
27. Kohlwein SD. Obese and anorexic yeasts: experimental models to understand the metabolic
syndrome and lipotoxicity. Biochim Biophys Acta 2010; 1801(3):222-9. doi:
10.1016/j.bbalip.2009.12.016.
28. Low KL, Shui G, Natter K, Yeo WK, Kohlwein SD, Dick T, Rao SP, Wenk MR. Lipid dropletassociated proteins are involved in the biosynthesis and hydrolysis of triacylglycerol in
Mycobacterium bovis bacillus Calmette-Guerin. J Biol Chem 2010; 285(28):21662-70. doi:
10.1074/jbc.M110.135731.
29. Rockenfeller P, Ring J, Muschett V, Beranek A, Buettner S, Carmona-Gutierrez D, Eisenberg
T, Khoury C, Rechberger G, Kohlwein SD, Kroemer G, Madeo F. Fatty acids trigger
mitochondrion-dependent necrosis. Cell Cycle 2010; 9(14):2836-42.
30. Youn JY, Friesen H, Kishimoto T, Henne WM, Kurat CF, Ye W, Ceccarelli DF, Sicheri F,
Kohlwein SD, McMahon HT, Andrews BJ. Dissecting BAR domain function in the yeast
Amphiphysins Rvs161 and Rvs167 during endocytosis. Mol Biol Cell 2010; 21(17):3054-69.
doi: 10.1091/mbc.E10-03-0181.
31. Almsherqi ZA, Landh T, Kohlwein SD, Deng Y. Chapter 6: cubic membranes the missing
dimension of cell membrane organization. Int Rev Cell Mol Biol 2009; 274:275-342. doi:
10.1016/S1937-6448(08)02006-6.
32. Beranek A, Rechberger G, Knauer H, Wolinski H, Kohlwein SD, Leber R. Identification of a
cardiolipin-specific phospholipase encoded by the gene CLD1 (YGR110W) in yeast. J Biol
Chem 2009; 284(17):11572-8. doi: 10.1074/jbc.M805511200.
33. Deng Y, Almsherqi ZA, Shui G, Wenk MR, Kohlwein SD. Docosapentaenoic acid (DPA) is a
critical determinant of cubic membrane formation in amoeba Chaos mitochondria. FASEB J
2009; 23(9):2866-71. doi: 10.1096/fj.09-130435.
34. Kurat CF, Wolinski H, Petschnigg J, Kaluarachchi S, Andrews B, Natter K, Kohlwein SD.
Cdk1/Cdc28-dependent activation of the major triacylglycerol lipase Tgl4 in yeast links
lipolysis
to
cell-cycle
progression.
Mol
Cell
2009;
33(1):53-63.
doi:
10.1016/j.molcel.2008.12.019.
35. Petschnigg J, Wolinski H, Kolb D, Zellnig G, Kurat CF, Natter K, Kohlwein SD. Good fat,
essential cellular requirements for triacylglycerol synthesis to maintain membrane
homeostasis in yeast. J Biol Chem 2009; 284(45):30981-93. doi: 10.1074/jbc.M109.024752.
36. Schreiber R, Taschler U, Wolinski H, Seper A, Tamegger SN, Graf M, Kohlwein SD,
Haemmerle G, Zimmermann R, Zechner R, Lass A. Esterase 22 and beta-glucuronidase
hydrolyze retinoids in mouse liver. J Lipid Res 2009; 50(12):2514-23. doi:
10.1194/jlr.M000950.
37. Wolinski H, Natter K, Kohlwein SD. The fidgety yeast: focus on high-resolution live yeast cell
microscopy. Methods Mol Biol 2009; 548:75-99. doi: 10.1007/978-1-59745-540-4_5.
38. Wolinski H, Petrovic U, Mattiazzi M, Petschnigg J, Heise B, Natter K, Kohlwein SD. Imagingbased live cell yeast screen identifies novel factors involved in peroxisome assembly. J
Proteome Res 2009; 8(1):20-7. doi: 10.1021/pr800782n.