pdf CIMAR - Evaluation Report

Transcrição

ACTIVITY
REPORT 2009
Content: Annual Activity Report 2009, presented by CIMAR – Associated Laboratory to
Fundação para a Ciência e a Tecnologia.
LIST OF CONTENTS
1. GENERAL INFORMATION ....................................................................................................1
2. RESEARCH TEAM ................................................................................................................2
3. OBJECTIVES AND ACHIEVEMENTS ..................................................................................... 13
4. ACTIVITIES ....................................................................................................................... 17
5. FUNDING ......................................................................................................................... 20
6. GENERAL INDICATORS ...................................................................................................... 21
7. RESEARCH LINES (THEMATIC AREAS)................................................................................. 23
RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems ...................................... 23
RL2 - Environmental Chemistry and Toxicology........................................................................ 31
RL3 - Biology and Marine Biotechnologies................................................................................ 38
RL4 - Aquaculture ...................................................................................................................... 45
8. RESEARCH GROUPS .......................................................................................................... 51
Aquaculture Research Group (Aquagroup) ............................................................................... 53
Biodiversity of Aquatic Ecosystems........................................................................................... 63
Biogeographical Ecology and Evolution (BEE) ........................................................................... 80
Biophysics .................................................................................................................................. 91
Cellular and Inorganic Biochemistry (CIB) ................................................................................. 97
Cellular, Molecular and Analytical Studies (LECEMA) ............................................................. 100
Chemical Speciation and Bioavailability (CS&B) ..................................................................... 111
Chemistry and Biological Activity of Marine Natural Products ............................................... 119
Comparative Molecular Endocrinology (CME) ........................................................................ 124
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH) .............................. 134
Ecophysiology.......................................................................................................................... 141
Ecotoxicology (ECOTOX) .......................................................................................................... 150
Ecotoxicology, Genomics and Evolution (LEGE) ...................................................................... 162
Environmental Technologies ................................................................................................... 172
Environmental Toxicology ....................................................................................................... 180
Evolution, Development and Gene Expression (EDGE)........................................................... 188
Fish Nutrition........................................................................................................................... 199
Fisheries Biology and Hydroecology Research Group (FBHRG) .............................................. 205
Fisheries, Biodiversity and Conservation ................................................................................ 213
Geology and Paleoceanography ............................................................................................. 224
Hydrobiology ........................................................................................................................... 235
Marine Biotechnologies (MarBiotech) .................................................................................... 245
Marine Plant Ecology (ALGAE) ................................................................................................ 251
Microbial Ecology and Evolution ............................................................................................. 262
Nutrition, Growth and Quality of Fish (LANUCE) .................................................................... 267
Oceanic and Coastal Dynamics ............................................................................................... 278
Pathology ................................................................................................................................ 288
Plant Systematics and Bioinformatics ..................................................................................... 297
Synthesis and Organic Reactivity ............................................................................................ 301
9. OTHER LA ACTIVITIES...................................................................................................... 306
9a. Services and resources ...................................................................................................... 306
9b. Networking actions ........................................................................................................... 308
9c. Training .............................................................................................................................. 310
9d. Outreach/science and society ........................................................................................... 311
9e. International events .......................................................................................................... 314
10. INTERNAL EVALUATIONS .............................................................................................. 316
11. FUTURE OBJECTIVES ..................................................................................................... 317
SCIENTIFIC OUTPUT 2009 (Publications, Advanced Training, Communications, Patents)
Evaluation Report 2009
1. GENERAL INFORMATION
1.1 Name of Research Unit
CIMAR - Centro de Investigação Marinha e Ambiental
1.2 Coordinator
João José Oliveira Dias Coimbra
1.3 Main Scientific Domain
Marine Sciences
1.4 Leading Hosting Institution
Centro Interdisciplinar de Investigação Marinha e Ambiental – CIIMAR
1.5 Other Insitutions involved
Centro de Ciências do Mar – CCMAR
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2. RESEARCH TEAM
2.1 Member researchers with PhD
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2
1. Adelino Vicente Mendonça Canário (Agregação / Professor Catedrático / 50%)
2. Aires Manuel Pereira Oliva Teles (Agregação / Professor Catedrático / 50%)
3. Anake Kijjoa (Agregação / Professor Catedrático / 60%)
4. Carlos José Correia de Azevedo (Agregação / Professor Catedrático / 80%)
5. Deborah Mary Power (Agregação / Professor Catedrático / 50%)
6. Eduardo Jorge Sousa Rocha (Agregação / Professor Catedrático / 40%)
7. Emídio Ferreira Santos Gomes (Agregação / Professor Catedrático / 50%)
8. Fernando Francisco Machado Veloso Gomes (Agregação / Professor Catedrático / 60%)
9. Helena Maria Leite Pato Granja (Agregação / Professor Catedrático / 50%)
10. João José Oliveira Dias Coimbra (Agregação / Professor Catedrático / 70%)
11. Jorge Guimarães da Costa Eiras (Agregação / Professor Catedrático / 50%)
12. José Pedro de Andrade e Silva Andrade (Agregação / Professor Catedrático / 50%)
13. Lúcia Maria das Candeias Guilhermino (Agregação / Professor Catedrático / 60%)
14. Maria Armanda Reis Henriques (Agregação / Professor Catedrático / 50%)
15. Maria Teresa Dinis (Agregação / Professor Catedrático / 75%)
16. Maria Teresa Sá Dias de Vasconcelos (Agregação / Professor Catedrático / 40%)
17. Rogério Alves Ferreira Monteiro (Agregação / Professor Catedrático / 40%)
18. Vitor Manuel Oliveira Vasconcelos (Agregação / Professor Catedrático / 70%)
19. Adriano Agostinho Donas Bôto Bordalo e Sá (Agregação / Professor Associado / 40%)
20. Alexandre Manuel Silva Lobo Cunha (Agregação / Professor Associado / 40%)
21. António José Rogeiro Gouveia (Agregação / Professor Associado / 50%)
22. Francisco de Almeida Taveira Pinto (Agregação / Professor Associado / 50%)
23. Maria Leonor Nunes Ribeiro Cruzeiro (Agregação / Professor Associado / 50%)
24. Maria Leonor Quintais Cancela Fonseca (Agregação / Professor Associado / 50%)
25. Maria Lurdes Santos Cristiano (Agregação / Professor Associado / 50%)
26. Rui Orlando Pimenta Santos (Agregação / Professor Associado / 50%)
27. Ana Isabel de Melo Azevedo Neto (Agregação / Professor Auxiliar / 35%)
28. Aurélia Maria de Pinho Marques Saraiva (Agregação / Professor Auxiliar / 50%)
29. Maria Natividade Ribeiro Vieira (Agregação / Professor Auxiliar / 50%)
30. Paulo José de Azevedo Pinto Rema (Agregação / Professor Auxiliar / 50%)
31. Fernanda Russell Pinto (Doutoramento / Professor Associado / 60%)
32. Isabel Maria Trigueiros Sousa Pinto Machado (Doutoramento / Professor Associado / 50%)
33. João Paulo de Sousa Cabral (Doutoramento / Professor Associado / 35%)
34. Jorge Pereira Machado (Doutoramento / Professor Associado / 50%)
35. Karim Erzini (Doutoramento / Professor Associado / 33%)
36. Luísa Maria Pinheiro Valente (Doutoramento / Professor Associado / 75%)
37. Manuel Aureliano Pereira Martins Alves (Doutoramento / Professor Associado / 50%)
38. Maria Alexandra Anica Teodósio Chicharo (Doutoramento / Professor Associado / 50%)
39. Maria João Tome Costa Sousa Rocha (Doutoramento / Professor Associado / 40%)
40. Maria Margarida M Castro (Doutoramento / Professor Associado / 50%)
41. Paulo Manuel Rodrigues Vaz-Pires (Doutoramento / Professor Associado / 50%)
42. Pedro Manuel Silva Duarte (Doutoramento / Professor Associado / 20%)
43. Rodrigo Jorge Fonseca de Oliveira Maia (Doutoramento / Professor Associado / 60%)
44. Alberto Teodorico Rodrigues Moura Correia (Doutoramento / Professor Auxiliar / 30%)
45. Ana Carolina Tavares Estima Santos da Cunha Feming (Doutoramento / Professor Auxiliar /
40%)
46. António Paulo Alves Ferreira de Carvalho (Doutoramento / Professor Auxiliar / 50%)
47. Carla Batista Carvalho Batista Pinto (Doutoramento / Professor Auxiliar / 25%)
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48. Cristina Maria Bravo de Faria Cruz (Doutoramento / Professor Auxiliar / 50%)
49. Dina Cristina Fernandes Rodrigues da Costa Simes (Doutoramento / Professor Auxiliar / 50%)
50. Eduardo Nuno Picoto Lopes Barata (Doutoramento / Professor Auxiliar / 50%)
51. Filipe Alexandre Oliveira dos Santos Alberto (Doutoramento / Professor Auxiliar / 50%)
52. Gerhard Michael Weber (Doutoramento / Professor Auxiliar / 30%)
53. Graça Maria Figueiredo Casal (Doutoramento / Professor Auxiliar / 50%)
54. João Carlos Serafim Varela (Doutoramento / Professor Auxiliar / 50%)
55. João Pedro Gomes Moreira Pêgo (Doutoramento / Professor Auxiliar / 60%)
56. José Alberto Álvares Pereira Gonçalves (Doutoramento / Professor Auxiliar / 30%)
57. José Américo Pereira de Sousa (Doutoramento / Professor Auxiliar / 50%)
58. José Carlos Fernandes Antunes (Doutoramento / Professor Auxiliar / 50%)
59. José Fernando Magalhães Gonçalves (Doutoramento / Professor Auxiliar / 30%)
60. Luís Filipe Pereira de Oliva Teles (Doutoramento / Professor Auxiliar / 50%)
61. Luisa Paula Viola Afonso Barreira (Doutoramento / Professor Auxiliar / 50%)
62. Manfred Josef Kaufmann (Doutoramento / Professor Auxiliar / 40%)
63. Margarida de Lurdes de Jesus Bastos Cristo (Doutoramento / Professor Auxiliar / 50%)
64. Maria Antónia Santos Mendes Salgado (Doutoramento / Professor Auxiliar / 35%)
65. Maria Clara Gomes Quadros Lázaro da Silva (Doutoramento / Professor Auxiliar / 50%)
66. Maria Clara Ramalho Monteiro Pires Basto (Doutoramento / Professor Auxiliar / 50%)
67. Maria Clara Semedo da Silva Costa (Doutoramento / Professor Auxiliar / 40%)
68. Maria Ester Tavares Alvares Serrao (Doutoramento / Professor Auxiliar / 50%)
69. Maria Joana Afonso Pereira Fernandes (Doutoramento / Professor Auxiliar / 50%)
70. Maria João Faria Leite Dias dos Santos (Doutoramento / Professor Auxiliar / 50%)
71. Maria Manuela Fraga Juliano (Doutoramento / Professor Auxiliar / 50%)
72. Maria Margarida da Fonseca e Castro Cardoso (Doutoramento / Professor Auxiliar / 30%)
73. Maria Teresa Calvinho Cerveira Borges (Doutoramento / Professor Auxiliar / 50%)
74. Paula Cristina Paulo Videira da Silva (Doutoramento / Professor Auxiliar / 40%)
75. Paulo Alexandre de Avilez Rodrigues de Almeida Valente (Doutoramento / Professor Auxiliar /
60%)
76. Paulo José Relvas Almeida (Doutoramento / Professor Auxiliar / 50%)
77. Paulo José Talhadas Santos (Doutoramento / Professor Auxiliar / 50%)
78. Paulo Manuel Rodrigues Martins da Costa (Doutoramento / Professor Auxiliar / 50%)
79. Pedro Miguel Leal Rodrigues (Doutoramento / Professor Auxiliar / 50%)
80. Rita Castilho (Doutoramento / Professor Auxiliar / 50%)
81. Ronaldo Gomes de Sousa (Doutoramento / Professor Auxiliar / 30%)
82. Sérgio dos Reis Marques Madeira (Doutoramento / Professor Auxiliar / 30%)
83. Teresa Isabel Mendonça Modesto (Doutoramento / Professor Auxiliar / 50%)
84. Albina Dolores Cardoso da Silva Castro Resende (Doutoramento / Professor Coordenador /
25%)
85. Maria Dulce da Mota Antunes de Oliveira Estêvão (Doutoramento / Professor Coordenador /
40%)
86. Maria Alexandra de Sousa Lourenço Nunes (Doutoramento / Professor-Adjunto / 50%)
87. Eduardo Bruno Oliveira Esteves (Doutoramento / Professor-Adjunto Equiparado / 50%)
88. Maria Luisa Machado Cerqueira Bastos (Doutoramento / Investigador Principal / 50%)
89. Agostinho Antunes Pereira (Doutoramento / Investigador Auxiliar / 100%)
90. Alexandre Marmoto de Oliveira Campos (Doutoramento / Investigador Auxiliar / 100%)
91. Ana Maria Ferreira Bio (Doutoramento / Investigador Auxiliar / 100%)
92. Ana Paula de Campos Mucha (Doutoramento / Investigador Auxiliar / 100%)
93. Carlos Alexandre Sarabando Gravato (Doutoramento / Investigador Auxiliar / 100%)
94. Channarayapattana Narasimhamurthy Prabhu (Doutoramento / Investigador Auxiliar / 100%)
95. Cristina Marisa Ribeiro de Almeida (Doutoramento / Investigador Auxiliar / 100%)
96. Cymon John Cox (Doutoramento / Investigador Auxiliar / 100%)
97. Elsa Maria Branco Froufe Andrade (Doutoramento / Investigador Auxiliar / 100%)
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98. Fernando José Tuya Cortés (Doutoramento / Investigador Auxiliar / 100%)
99. Francisco Arenas Parra (Doutoramento / Investigador Auxiliar / 100%)
100. Gareth Anthony Pearson (Doutoramento / Investigador Auxiliar / 100%)
101. Iacopo Bertocci (Doutoramento / Investigador Auxiliar / 100%)
102. Isabel Gonçalves de Barbosa Araújo (Doutoramento / Investigador Auxiliar / 100%)
103. João Carlos dos Reis Cardoso (Doutoramento / Investigador Auxiliar / 100%)
104. João Miguel Sousa da Silva (Doutoramento / Investigador Auxiliar / 100%)
105. Jonathan Mark Wilson (Doutoramento / Investigador Auxiliar / 100%)
106. Jorge Manuel dos Santos Gonçalves (Doutoramento / Investigador Auxiliar / 100%)
107. Jorge Proença Dias (Doutoramento / Investigador Auxiliar / 100%)
108. Juan Fuentes Diaz (Doutoramento / Investigador Auxiliar / 100%)
109. Kim Richard Larsen (Doutoramento / Investigador Auxiliar / 100%)
110. Laura Maria Simões Coutinho Guimarães (Doutoramento / Investigador Auxiliar / 100%)
111. Luís Eugénio Castanheira da Conceição (Doutoramento / Investigador Auxiliar / 100%)
112. Luís Filipe Costa Castro (Doutoramento / Investigador Auxiliar / 100%)
113. Machiel Simon Bos (Doutoramento / Não aplicável (bolseiro) / 100%)
114. Maria Helena Tabuaço Rêgo Martins Peres (Doutoramento / Investigador Auxiliar / 100%)
115. Marta Mega Rufino (Doutoramento / Investigador Auxiliar / 100%)
116. Miguel Alberto Fernandes Machado e Santos (Doutoramento / Investigador Auxiliar / 100%)
117. Paulo Jorge Travessa Gavaia (Doutoramento / Investigador Auxiliar / 100%)
118. Pedro Miguel Guerreiro Costa Guerreiro (Doutoramento / Investigador Auxiliar / 100%)
119. Peter Colin Hubbard (Doutoramento / Investigador Auxiliar / 100%)
120. Radhouane Ben Hamadou (Doutoramento / Investigador Auxiliar / 100%)
121. Ralph Urbatzka (Doutoramento / Investigador Auxiliar / 100%)
122. Rodrigo da Silva Costa (Doutoramento / Investigador Auxiliar / 100%)
123. Rodrigo Ozório (Doutoramento / Investigador Auxiliar / 100%)
124. Rui Miguel Andrade Caldeira (Doutoramento / Investigador Auxiliar / 100%)
125. Susana Maria Pereira da Costa Moreira (Doutoramento / Investigador Auxiliar / 100%)
126. Vicent Laizé (Doutoramento / Investigador Auxiliar / 100%)
127. Rosa Carmina Cervantes Cianca (Doutoramento / Investigador / 100%)
128. Alexandra Paula Mimoso Henriques Cunha (Doutoramento / Não aplicável (bolseiro) /
100%)
129. Alfredo Nuno Damasceno Pinto de Oliveira (Doutoramento / Não aplicável (bolseiro) /
100%)
130. Ana Alexandra Pedrosa Ramos (Doutoramento / Não aplicável (bolseiro) / 100%)
131. Ana Filipa Naughton Henriques Andrez (Doutoramento / Não aplicável (bolseiro) / 100%)
132. Ana Luisa Frazão Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
133. Ana Mafalda Saraiva Baptista (Doutoramento / Não aplicável (bolseiro) / 100%)
134. António de Vilhena Andrade Ferreira Sykes (Doutoramento / Não aplicável (bolseiro) /
100%)
135. Aschwin Hillebrand Engelen (Doutoramento / Não aplicável (bolseiro) / 100%)
136. Carla Marília Abreu Freitas (Doutoramento / Não aplicável (bolseiro) / 100%)
137. Catarina Isabel de Matos Martins (Doutoramento / Não aplicável (bolseiro) / 100%)
138. Catarina Maria Pinto Mora Pinto de Magalhães (Doutoramento / Não aplicável (bolseiro) /
100%)
139. Cláudia Raquel Cêa de Aragão Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%)
140. Cristina Isabel Coelho Dias Lopes (Doutoramento / Não aplicável (bolseiro) / 100%)
141. Daniel António Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%)
142. Dulce Isabel Assis Alves Martins (Doutoramento / Não aplicável (bolseiro) / 100%)
143. Fernando Cánivas García (Doutoramento / Não aplicável (bolseiro) / 100%)
144. Holly Freedman (Doutoramento / Não aplicável (bolseiro) / 100%)
145. Isabel Cristina Guimarães Nogueira (Doutoramento / Não aplicável (bolseiro) / 100%)
146. Isabel Cristina Oliveira Gonzalez Cunha (Doutoramento / Não aplicável (bolseiro) / 100%)
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147. Isabel Martins Gil (Doutoramento / Não aplicável (bolseiro) / 100%)
148. Joana Costa Vilhena de Bessa Campos (Doutoramento / Não aplicável (bolseiro) / 100%)
149. Joana Fernandes da Fonseca da Costa Martins Osswald (Doutoramento / Não aplicável
(bolseiro) / 100%)
150. Joana Ferreira Marques Ferreira Cardoso (Doutoramento / Não aplicável (bolseiro) / 100%)
151. João Lemos Gomes Canning Clode (Doutoramento / Não aplicável (bolseiro) / 100%)
152. Jorge Afonso Martins da Palma (Doutoramento / Não aplicável (bolseiro) / 100%)
153. Justyna Kopecka-Pilarczyk (Doutoramento / Não aplicável (bolseiro) / 100%)
154. Katy Nicastro (Doutoramento / Não aplicável (bolseiro) / 100%)
155. Larraitz Garmendia (Doutoramento / Não aplicável (bolseiro) / 100%)
156. Laurence Alexandrine Mathilde Deloffre (Doutoramento / Não aplicável (bolseiro) / 100%)
157. Ledicia Rey Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%)
158. Leonardo Filipe Rodrigues da Mata (Doutoramento / Não aplicável (bolseiro) / 100%)
159. Luís Miguel dos Santos Russo Vieira (Doutoramento / Não aplicável (bolseiro) / 100%)
160. Luisa Margarida Batista Custodio (Doutoramento / Não aplicável (bolseiro) / 100%)
161. Manuel Gesto Rodríguez (Doutoramento / Não aplicável (bolseiro) / 100%)
162. Marcelino Miguel Guedes de Jesus Oliveira (Doutoramento / Não aplicável (bolseiro) /
100%)
163. Marcos Rubal Garcia (Doutoramento / Não aplicável (bolseiro) / 100%)
164. María Asunción Lago Lestón (Doutoramento / Não aplicável (bolseiro) / 100%)
165. Maria de Fátima Azevedo Alexandrino Fernandes (Doutoramento / Não aplicável (bolseiro) /
100%)
166. Maria del Mar Huertas Pau (Doutoramento / Não aplicável (bolseiro) / 100%)
167. Maria Elena Varela Alvarez (Doutoramento / Não aplicável (bolseiro) / 100%)
168. Maria Emília Carvalho Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%)
169. Maria Regina Oliveira Lopes da Cunha (Doutoramento / Não aplicável (bolseiro) / 100%)
170. Maria Teresa Garrett Silveirinha Sottomayor Neuparth (Doutoramento / Não aplicável
(bolseiro) / 100%)
171. Marta Sofia Sá Ferreira (Doutoramento / Não aplicável (bolseiro) / 100%)
172. Nadège Richard (Doutoramento / Não aplicável (bolseiro) / 100%)
173. Natércia Maria da Silva Conceição (Doutoramento / Não aplicável (bolseiro) / 100%)
174. Nikolay Kolmakov (Doutoramento / Não aplicável (bolseiro) / 100%)
175. Nuna Cláudia Peixoto de Araújo (Doutoramento / Não aplicável (bolseiro) / 100%)
176. Onno Everhardus Diekmann (Doutoramento / Não aplicável (bolseiro) / 100%)
177. Patrícia Isabel Silvestre Pinto (Doutoramento / Não aplicável (bolseiro) / 100%)
178. Paula Cristina Enes Oliveira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)
179. Pedro Alexandre Garcia Range (Doutoramento / Não aplicável (bolseiro) / 100%)
180. Pedro Miguel Coutinho Victorino Borges Morais (Doutoramento / Não aplicável (bolseiro) /
100%)
181. Rawiwan Watanadilok (Doutoramento / Não aplicável (bolseiro) / 100%)
182. Rita Alexandra Duarte Borges (Doutoramento / Não aplicável (bolseiro) / 100%)
183. Rui Manuel do Amaral Branco de Oliveira Quartau (Doutoramento / Não aplicável (bolseiro)
/ 100%)
184. Rui Pedro Andrade Coelho (Doutoramento / Não aplicável (bolseiro) / 100%)
185. Rui Pedro Gonçalves Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
186. Rute Andreia Rodrigues da Fonseca (Doutoramento / Não aplicável (bolseiro) / 100%)
187. Sandra Cristina da Costa e Silva Ramos (Doutoramento / Não aplicável (bolseiro) / 100%)
188. Sara Judite Lopes Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%)
189. Sara Maria Mira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)
190. Simone Cavenati (Doutoramento / Não aplicável (bolseiro) / 100%)
191. Sofia Alexandra Dias Engrola (Doutoramento / Não aplicável (bolseiro) / 100%)
192. Sonia Martinez Paramo (Doutoramento / Não aplicável (bolseiro) / 100%)
193. Susana Isabel dos Santos Cabaço (Doutoramento / Não aplicável (bolseiro) / 100%)
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194. Susana Rodrigues Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
195. Teodor Ludmilov Stoichev (Doutoramento / Não aplicável (bolseiro) / 100%)
196. Teresa Alexandra Ribeiro Rodrigues (Doutoramento / Não aplicável (bolseiro) / 100%)
197. Teresa Paula Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%)
198. Tida Dethoup (Doutoramento / Não aplicável (bolseiro) / 100%)
199. Vijayakumar Parameswaran (Doutoramento / Não aplicável (bolseiro) / 100%)
200. Zardi Gerardo (Doutoramento / Não aplicável (bolseiro) / 100%)
201. Ana Margarida Araújo Amaral (Doutoramento / Técnico Superior e Técnico / 100%)
202. Carlos Gil Ribeiro Martins (Doutoramento / Técnico Superior e Técnico / 100%)
203. Paula Raquel da Silva Jorge Coutinho Ferreira (Doutoramento / Técnico Superior e Técnico /
100%)
2.2 Member researchers without PhD
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6
1. Luís Gabriel da Silva Luís (Mestrado / Estagiário de Investigação / 50%)
2. Patrícia Alexandra Coreia Oliveira (Mestrado / Estagiário de Investigação / 100%)
3. Helena Isabel Costa de Oliveira (Mestrado / Não aplicável (bolseiro)/ 100%)
4. Allan Tainá de Souza (Mestrado / Não aplicável (bolseiro)/ 100%)
5. Amélia Cláudia Figueiredo Silva (Mestrado / Não aplicável (bolseiro) / 100%)
6. Ana Isabel Delfin dos Santos Alexandre (Mestrado / Não aplicável (bolseiro) / 100%)
7. Ana Sofia Cardoso Mesquita (Mestrado / Não aplicável (bolseiro)/ 100%)
8. André Sucena Afonso (Mestrado / Não aplicável (bolseiro)/ 100%)
9. Ângela Alexandra Martinho Ramos (Mestrado / Não aplicável (bolseiro)/ 100%)
10. António José Mendes Martins (Mestrado / Não aplicável (bolseiro) / 100%)
11. Aurélie Pinto Rodrigues (Mestrado / Não aplicável (bolseiro)/ 100%)
12. Bárbara Bilreiro de Mendoça Frazão (Mestrado / Não aplicável (bolseiro)/ 100%)
13. Benjamín Costas Refojos (Mestrado / Não aplicável (bolseiro) / 100%)
14. Carla Alexandra da Silva Monteiro (Mestrado / Não aplicável (bolseiro) / 100%)
15. Catarina da Rocha Cruzeiro (Mestrado / Não aplicável (bolseiro)/ 100%)
16. Catarina Fernanda de Carvalho Pinheiro Teixeira (Mestrado / Não aplicável (bolseiro) / 100%)
17. Catarina Figueiredo da Mota (Mestrado / Não aplicável (bolseiro) / 100%)
18. Cristiana Ivone Tavares Moreira (Mestrado / Não aplicável (bolseiro)/ 100%)
19. Cristiane Cassiolato Pires Hardoim (Mestrado / Não aplicável (bolseiro)/ 100%)
20. Cristina Maria Simões de Jesus Rocha (Mestrado / Não aplicável (bolseiro) / 100%)
21. David Maria Aguiar Abecasis (Mestrado / Não aplicável (bolseiro) / 100%)
22. David Veríssimo Piló (Mestrado / Não aplicável (bolseiro) / 100%)
23. Diogo Filipe da Silva Gonçalves Soares Paulo (Mestrado / Não aplicável (bolseiro) / 100%)
24. Edgar Francisco Rosas Alquicira (Mestrado / Não aplicável (bolseiro)/ 100%)
25. Fátima de Carvalho Vaz Pinto (Mestrado / Não aplicável (bolseiro) / 100%)
26. Filipa soares Rocha (Mestrado / Não aplicável (bolseiro)/ 100%)
27. Francisco Cid Gonçalves Mont’Alverne Rocha Pires (Mestrado / Não aplicável (bolseiro)/
100%)
28. Gonçalo Nuno Santos Neto da Graça (Mestrado / Não aplicável (bolseiro) / 100%)
29. Guilherme Moura Paredes (Mestrado / Não aplicável (bolseiro)/ 100%)
30. Guillermin Aguero Chapin (Mestrado / Não aplicável (bolseiro) / 100%)
31. Gustavo Oliveira de Meneses Martins (Mestrado / Não aplicável (bolseiro)/ 100%)
32. Inês Maria dos Santos Guerreiro (Mestrado / Não aplicável (bolseiro) / 100%)
33. Joana do Passo Carneiro Azevedo (Mestrado / Não aplicável (bolseiro)/ 100%)
34. Joana Fernandez de Carvalho (Mestrado / Não aplicável (bolseiro)/ 100%)
35. Joana Ferreira Costa (Mestrado / Não aplicável (bolseiro) / 100%)
36. Joana Isabel Correia Bondoso (Mestrado / Não aplicável (bolseiro) / 100%)
37. Joana Maria Reis Franco Cruz (Mestrado / Não aplicável (bolseiro) / 100%)
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38. João Luis Vargas de Almeida Saraiva (Mestrado / Não aplicável (bolseiro)/ 100%)
39. José Carlos de Morais Martins (Mestrado / Não aplicável (bolseiro) / 100%)
40. Juliana Marina Moreira Mendes (Mestrado / Não aplicável (bolseiro)/ 100%)
41. Karla León-Cisneros (Mestrado / Não aplicável (bolseiro)/ 100%)
42. Laura Diniz Leite (Mestrado / Não aplicável (bolseiro)/ 100%)
43. Leonor Isabel Moreira de Araújo (Mestrado / Não aplicável (bolseiro) / 100%)
44. Luciana Paiva das Neves (Mestrado / Não aplicável (bolseiro) / 100%)
45. Mafalda Rangel Malheiro Dias de Oliveira (Mestrado / Não aplicável (bolseiro) / 100%)
46. Mahaut Diane Marie Stephanie de Labroue de Vareilles Sommieres (Mestrado / Não aplicável
(bolseiro) / 100%)
47. Margarida Dulce da Conceição Aragão Hermida (Mestrado / Não aplicável (bolseiro)/ 100%)
48. Maria Alexandra Santos Martins (Mestrado / Não aplicável (bolseiro)/ 100%)
49. Maria Catarina Murteira Rico dos Santos Campos (Mestrado / Não aplicável (bolseiro) /
100%)
50. Maria Filipa Bento de Oliveira Falcão Castanheira (Mestrado / Não aplicável (bolseiro)/ 100%)
51. Mariana Fonseca Hinzmann (Mestrado / Não aplicável (bolseiro) / 100%)
52. Martina Di Iulio Ilarri (Mestrado / Não aplicável (bolseiro)/ 100%)
53. Micaela de Fátima da Rocha Mota (Mestrado / Não aplicável (bolseiro)/ 100%)
54. Miguel Filipe Ferreira da Costa Ramos (Mestrado / Não aplicável (bolseiro) / 100%)
55. Miguel José Teodoro Correia (Mestrado / Não aplicável (bolseiro) / 100%)
56. Mirjam Susanne van de Vliet (Mestrado / Não aplicável (bolseiro) / 100%)
57. Monya Mendes Costa (Mestrado / Não aplicável (bolseiro) / 100%)
58. Nelson Alexandre Castilho Coelho (Mestrado / Não aplicável (bolseiro)/ 100%)
59. Nelson Ribeiro Pires (Mestrado / Não aplicável (bolseiro)/ 100%)
60. Odete Marinho Gonçalves (Mestrado / Não aplicável (bolseiro) / 100%)
61. Parthibaraj Anoop Alex (Mestrado / Não aplicável (bolseiro)/ 100%)
62. Pedro Alexandre Coelho Borges (Mestrado / Não aplicável (bolseiro) / 100%)
63. Pedro Neves de Carvalho (Mestrado / Não aplicável (bolseiro) / 100%)
64. Pedro Nuno da Costa Leão (Mestrado / Não aplicável (bolseiro) / 100%)
65. Renata Isabel de Sousa Gonçalves (Mestrado / Não aplicável (bolseiro)/ 100%)
66. Ricardo Manuel Rafael Afonso (Mestrado / Não aplicável (bolseiro) / 100%)
67. Ricardo Mário Bastos Leite (Mestrado / Não aplicável (bolseiro) / 100%)
68. Richard Deurloo (Mestrado / Não aplicável (bolseiro) / 100%)
69. Siby Philip (Mestrado / Não aplicável (bolseiro)/ 100%)
70. Silvia Alexandra Pereira Lourenço (Mestrado / Não aplicável (bolseiro)/ 100%)
71. Sofia Ester e Sousa de Aguilar Dias (Mestrado / Não aplicável (bolseiro)/ 100%)
72. Sofia Gabriel Garcia Santos (Mestrado / Não aplicável (bolseiro) / 100%)
73. Sónia Maria de Sousa Olim (Mestrado / Não aplicável (bolseiro) / 100%)
74. Susana Maria Rocha Pina (Mestrado / Não aplicável (bolseiro) / 100%)
75. Vera Alexandra Garcia da Fonseca Batista (Mestrado / Não aplicável (bolseiro) / 100%)
76. Vitor Oliveira Lopes (Mestrado / Não aplicável (bolseiro)/ 100%)
77. Ana Rita Ferreira Patarra (Mestrado / Não aplicável (estudante)/ 100%)
78. Maria da Piedade Moreira Brandão (Mestrado / Não aplicável (estudante)/ 100%)
79. Marisa Alexandra Marques de Freitas (Mestrado / Não aplicável (estudante)/ 100%)
80. Rute Sofia Borlido Fiúza Fernandes Pinto (Mestrado / Não aplicável (estudante)/ 100%)
81. Vânia de Jesus de Paiva Freitas (Mestrado / Não aplicável (estudante)/ 100%)
82. Ana Sousa Ramos Ramalho Ribeiro de Magalhães Sant’Ana (Mestrado / Técnico Superior e
Técnico/ 100%)
83. Beatriz Maria Alvega Cardoso (Mestrado / Técnico Superior e Técnico/ 100%)
84. Cristina Maria Viegas Inácio (Mestrado / Técnico Superior e Técnico/ 100%)
85. Emília da Glória Moreira Afonso (Mestrado / Técnico Superior e Técnico/ 100%)
86. Hugo Gabriel Maia da Silva Santos (Mestrado / Técnico Superior e Técnico/ 100%)
87. Marta Sofia Mendes Valente Bernardo (Mestrado / Técnico Superior e Técnico/ 100%)
7
-
8
88. Miguel Ângelo Salgado dos Santos (Mestrado / Técnico Superior e Técnico/ 80%)
89. Pedro Alexandre Pereira dos Reis (Mestrado / Técnico Superior e Técnico/ 100%)
90. Susana Micaela Machado Ferreira do Vale (Mestrado / Técnico Superior e Técnico/ 100%)
91. Afonso Costa Lucas Prestes (Licenciatura / Investigador / 100%)
92. Eunice Rute Magalhães Nogueira Xavier (Licenciatura / Investigador / 100%)
93. Maria Margarida Oliveira Maló Machado (Licenciatura / Investigador / 60%)
94. Nuno Miguel da Silva Ascensão Vaz Álvaro (Licenciatura / Investigador / 100%)
95. Amin Lopes Ismael (Licenciatura / Não aplicável (bolseiro) / 100%)
96. Ana Catarina Matos Passareiro (Licenciatura / Não aplicável (bolseiro) / 100%)
97. Ana Cristina Silva Rocha (Licenciatura / Não aplicável (bolseiro) / 100%)
98. Ana Filipa dos Santos Gonçalves (Licenciatura / Não aplicável (bolseiro) / 100%)
99. Ana Isabel Santos Couto (Licenciatura / Não aplicável (bolseiro) / 100%)
100. Ana Mafalda Rocha Tavares (Licenciatura / Não aplicável (bolseiro) / 100%)
101. Ana Margarida Pinto Henrique Machado (Licenciatura / Não aplicável (bolseiro) / 100%)
102. Ana Rita Andrade Coelho (Licenciatura / Não aplicável (bolseiro) / 100%)
103. Ana Rita Guillot Caldas (Licenciatura / Não aplicável (bolseiro) / 100%)
104. Anabela Ben-Simon Brito (Licenciatura / Não aplicável (bolseiro) / 100%)
105. Anabela Rodrigues Lopes (Licenciatura / Não aplicável (bolseiro) / 100%)
106. André Gonçalo Antunes dos Santos (Licenciatura / Não aplicável (bolseiro) 100%)
107. Andreia Cristina Domingues Bringela (Licenciatura / Não aplicável (bolseiro) / 100%)
108. Brigite Sandra Nunes Simões (Licenciatura / Não aplicável (bolseiro) / 100%)
109. Bruno Emanuel Pereira Louro (Licenciatura / Não aplicável (bolseiro) / 100%)
110. Carla Alexandra São Bento Viegas (Licenciatura / Não aplicável (bolseiro) / 100%)
111. Cindy Vitória Fazenda (Licenciatura / Não aplicável (bolseiro) / 100%)
112. Cláudia Sofia Patrão Beliz Rosa e Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
113. Daniela da Silva Lima (Licenciatura / Não aplicável (bolseiro) / 100%)
114. Diogo Ennes Ferreira Sayanda (Licenciatura / Não aplicável (bolseiro) / 100%)
115. Eduardo Bruno de Freitas Vivas (Licenciatura / Não aplicável (bolseiro) / 100%)
116. Elisabete Alexandra Dias de Matos (Licenciatura / Não aplicável (bolseiro) / 100%)
117. Eva Catarina Costa Amorim (Licenciatura / Não aplicável (bolseiro) / 100%)
118. Florbela Alexandra Silva Vieira Martins (Licenciatura / Não aplicável (bolseiro) / 100%)
119. Francisco Luís Wallenstein Faria e Maia de Macedo (Licenciatura / Não aplicável (bolseiro) /
100%)
120. Frederico Miguel Cunha de Oliveira (Licenciatura / Não aplicável (bolseiro) / 100%)
121. Gonçalo Jorge Franco Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
122. Helena Rita de Carvalho Ferraz Pedrosa Teodósio (Licenciatura / Não aplicável (bolseiro) /
100%)
123. Inês Isabel Gralho Correia de Sousa (Licenciatura / Não aplicável (bolseiro) / 100%)
124. Izabela Luiza Reis (Licenciatura / Não aplicável (bolseiro) / 100%)
125. Joana Alexandra Teixeira Rosa (Licenciatura / Não aplicável (bolseiro) / 100%)
126. Joana Faria da Costa (Licenciatura / Não aplicável (bolseiro) / 100%)
127. Joana Reis de Almeida (Licenciatura / Não aplicável (bolseiro) / 100%)
128. Joana Ruela Boavida (Licenciatura / Não aplicável (bolseiro) / 100%)
129. Joana Silveira Soares (Licenciatura / Não aplicável (bolseiro) / 100%)
130. João Paulo Rodrigues Machado (Licenciatura / Não aplicável (bolseiro) / 100%)
131. Lília Isabel Lameirinhas Cabral (Licenciatura / Não aplicável (bolseiro) / 100%)
132. Liliana Isabel Tome Dos Anjos Guerreiro (Licenciatura / Não aplicável (bolseiro) / 100%)
133. Manuel Peixoto de Magalhães Lopes Lima (Licenciatura / Não aplicável (bolseiro) / 100%)
134. Marc Fernandez Moron (Licenciatura / Não aplicável (bolseiro) / 100%)
135. Margarida Bacelar Oliveira Santos (Licenciatura / Não aplicável (bolseiro) / 100%)
136. Maria da Nazaré Parada Figueiredo de Sousa Couto (Licenciatura / Não aplicável (bolseiro) /
100%)
137. Maria Helena Trindade de Abreu (Licenciatura / Não aplicável (bolseiro) / 100%)
-
138. Maria Inês de Almeida Páscoa (Licenciatura / Não aplicável (bolseiro) / 100%)
139. Maria Inês Oliveira Pinto Coelho (Licenciatura / Não aplicável (bolseiro) / 100%)
140. Maria Joao Rodrigues Pereira (Licenciatura / Não aplicável (bolseiro) / 50%)
141. Marisa Sárria Pereira de Passos (Licenciatura / Não aplicável (bolseiro) / 100%)
142. Marta Isabel da Silva Rafael (Licenciatura / Não aplicável (bolseiro) / 100%)
143. Michael Nogueira Viegas (Licenciatura / Não aplicável (bolseiro) / 100%)
144. Monica Sofia Furtado Martins (Licenciatura / Não aplicável (bolseiro) / 100%)
145. Patricia Alexandra Cavaleiro Diogo (Licenciatura / Não aplicável (bolseiro) / 100%)
146. Patrícia Isabel da Mota e Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
147. Paulo César Nunes Pereira do Rêgo (Licenciatura / Não aplicável (bolseiro) / 50%)
148. Paulo Jorge Rosa Santos (Licenciatura / Não aplicável (bolseiro) / 100%)
149. Pedro Filipe dos Santos Palma (Licenciatura / Não aplicável (bolseiro) / 100%)
150. Pedro Filipe Duarte Alves da Veiga (Licenciatura / Não aplicável (bolseiro) / 100%)
151.Pedro Luís Martins de Castro Pinheiro (Licenciatura / Não aplicável (bolseiro) / 100%)
152. Ricardo Jorge Pereira Córdova Marcos (Licenciatura / Não aplicável (bolseiro) / 100%)
153. Rita de Sousa Braga e Sá (Licenciatura / Não aplicável (bolseiro) / 100%)
154. Rita Isabel Pontes Barbosa Colen (Licenciatura / Não aplicável (bolseiro) / 100%)
155. Ruben Flávio Pacheco Couto (Licenciatura / Não aplicável (bolseiro) / 100%)
156. Rui Alexandre Mendes Gonçalves Mendes (Licenciatura / Não aplicável (bolseiro) / 100%)
157. Rute Sofia Tavares Martins Brazona (Licenciatura / Não aplicável (bolseiro) / 100%)
158. Sandra Cláudia Matias Rodrigues (Licenciatura / Não aplicável (bolseiro) / 100%)
159. Sérgia Catarina de Amorim Costa Dias (Licenciatura / Não aplicável (bolseiro) / 100%)
160. Sheila Natalí Estrada Allis (Licenciatura / Não aplicável (bolseiro) / 100%)
161. Silvia Maria dos Santos Albano (Licenciatura / Não aplicável (bolseiro) / 100%)
162. Sónia Isabel Rodrigues Aldeia Sanches Massa (Licenciatura / Não aplicável (bolseiro) / 100%)
163. Susana Galante Correia Pinto de Oliveira (Licenciatura / Não aplicável (bolseiro) / 100%)
164. Tânia Raquel Santos Aires (Licenciatura / Não aplicável (bolseiro) / 100%)
165. Tânia Vieira Madureira (Licenciatura / Não aplicável (bolseiro) / 25%)
166. Tomé Pereira de Azevedo Santos Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
167. Vera Lúcia Fernandes Rodrigues (Licenciatura / Não aplicável (bolseiro) / 100%)
168. Vitor Manuel Capela Ramos (Licenciatura / Não aplicável (bolseiro) / 100%)
169. Viviana Raquel Vieira da Silva Lopes (Licenciatura / Não aplicável (bolseiro) / 100%)
170. Wilson Gabriel Poseiro Coutinho Pinto (Licenciatura / Não aplicável (bolseiro) / 100%)
171. Ana Margarida Guerreiro Pereira (Licenciatura / Não aplicável (estudante) / 100%)
172. Bernardo Bordalo Domingues dos Santos (Licenciatura / Não aplicável (estudante) / 25%)
173. Cristiana Andreia Valente Oliveira (Licenciatura / Não aplicável (estudante) / 100%)
174. Daniel João da Silva Tavares Duarte (Licenciatura / Não aplicável (estudante) / 50%)
175. Daniella Luiza Antunes de Campos Duarte (Licenciatura / Não aplicável (estudante) / 50%)
176. Elisabete Maria Freitas Alves (Licenciatura / Não aplicável (estudante) / 50%)
177. Fernanda Cristina Rodrigues Malhão Pereira (Licenciatura / Não aplicável (estudante) / 40%)
178. Filipa Isabel da Rocha Cardoso (Licenciatura / Não aplicável (estudante) / 50%)
179. Flávia Daniela Macedo Viana (Licenciatura / Não aplicável (estudante) / 50%)
180. Inês Sofia Afonso Cardoso (Licenciatura / Não aplicável (estudante) / 50%)
181. Ivone da Silva Pinheiro (Licenciatura / Não aplicável (estudante) / 50%)
182. João Alexandre Bastos Sousa (Licenciatura / Não aplicável (estudante) / 100%)
183. João Carlos Pereira Morais (Licenciatura / Não aplicável (estudante) / 100%)
184. João Faria de Oliveira Santos (Licenciatura / Não aplicável (estudante) / 100%)
185. João Rodrigo Gonçalves Goiana Mesquita (Licenciatura / Não aplicável (Estudante) / 10%)
186. Juliana Andreia Silva da Cunha (Licenciatura / Não aplicável (estudante) / 50%)
187. Marisa Pinto da Silva (Licenciatura / Não aplicável (estudante) / 100%)
188. Marta Susana Amaro dos Santos (Licenciatura / Não aplicável (estudante) / 25%)
189. Paula Alexandra Rodrigues e Araújo Guedes (Licenciatura / Não aplicável (estudante) / 50%)
190. Paulina Pinto Carvalho (Licenciatura / Não aplicável (estudante) / 50%)
9
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10
191. Ricardo Bruno de Araújo Severino (Licenciatura / Não aplicável (estudante) / 50%)
192. Ricardo Manuel Campinho Capela (Licenciatura / Não aplicável (Estudante) / 50%)
193. Rui Filipe de Ascensão Almeida (Licenciatura / Não aplicável (estudante) / 50%)
194. Samira Joussef Piña (Licenciatura / Não aplicável (estudante) / 100%)
195. Silva Araújo Santos (Licenciatura / Não aplicável (estudante) / 50%)
196. Sofia Lourenço dos Santos (Licenciatura / Não aplicável (estudante) / 50%)
197. Sofia Raquel Soares Mesquita (Licenciatura / Não aplicável (Estudante) / 100%)
198. Ana Isabel Carvalho Machado (Licenciatura / Técnico Superior e Técnico / 100%)
199. Andreia Sofia Pires Pinto (Licenciatura / Técnico Superior e Técnico / 100%)
200. Jimmy Melro James (Licenciatura / Técnico Superior e Técnico / 100%)
201. João Carlos Sendão Silva (Licenciatura / Técnico Superior e Técnico / 25%)
202. João Eugénio Bernardino Pena dos Reis (Licenciatura / Técnico Superior e Técnico / 100%)
203. Liliane Leman (Licenciatura / Técnico Superior e Técnico / 80%)
204. Maria Rosalina Ramos Oliveira Grilo (Licenciatura / Técnico Superior e Técnico / 100%)
205. Pedro António Nobre Soares Pinto das Neves (Licenciatura / Técnico Superior e Técnico /
100%)
206. Pedro Miguel Gama Cláudio (Licenciatura / Técnico Superior e Técnico / 100%)
207. Tânia Sofia da Silva Guerreiro (Licenciatura / Técnico Superior e Técnico / 100%)
208. Ana Catarina Sotomaior Neto Cerqueira (Bacharelato / Não aplicável (estudante) / 100%)
209. Flávio Levi Piedade Neto Guerra Fontes (Bacharelato / Não aplicável (estudante) / 50%)
210. Joana Catarina Pereira Jorge Fonseca da Rocha (Ensino Secundário / Estagiário de
Investigação / 50%)
211. Ana Moreira Silva (Ensino secundário / Não aplicável (bolseiro) / 25%)
212. Ana Rita Grilo Barradas (Ensino secundário / Não aplicável (bolseiro) / 20%)
212. Janina Soraia Góis Diogo (Ensino secundário / Não aplicável (bolseiro) / 20%)
213. Jaqueline Huet Marques Cochofel (Ensino secundário / Não aplicável (bolseiro) / 20%)
214. Marisa Sofia Sousa Oliveira (Ensino secundário / Não aplicável (bolseiro) / 20%)
215. Olga Mouteira Azevedo (Ensino secundário / Não aplicável (bolseiro) / 20%)
216. Ricardo José da Silva Castro (Ensino secundário / Não aplicável (bolseiro) / 25%)
217. Sara Raquel Monteiro dos Reis (Ensino secundário / Não aplicável (bolseiro) / 30%)
218. Sara Rita Madeira Teixeira (Ensino secundário / Não aplicável (bolseiro) / 20%)
219. Carlos Filipe Carvalho Pinheiro (Ensino secundário / Não aplicável (estudante) / 100%)
220. Francisco António Baptista Fernandes (Ensino secundário / Não aplicável (estudante) / 25%)
221. Joana Maria Soares Pereira (Ensino secundário / Não aplicável (estudante) / 100%)
222. Marcos Lobo de Sousa (Ensino secundário / Não aplicável (estudante) / 50%)
223. Tiago Manuel Santos Justo (Ensino secundário / Não aplicável (estudante) / 20%)
224. Ana Patricia da Silva Todo Bom (Ensino secundário / Técnico Superior e Técnico / 100%)
225. António Marco Oliveira Cunha (Ensino secundário / Técnico Superior e Técnico / 100%)
226. Filipa Isabel Mourão Costa Queiroz (Ensino secundário / Técnico Superior e Técnico / 100%)
227. Helena Sofia Fernandes Teixeira (Ensino secundário / Técnico Superior e Técnico / 100%)
228. Marcos António Viegas Granja (Ensino secundário / Técnico Superior e Técnico / 100%)
229. Verónica Cristina Pereira Mascarenhas (Ensino secundário / Técnico Superior e Técnico /
100%)
2.3 Summary
Figure 1. Distribution of Member researchers according to academic degree.
Figure 2. Distribution of Member researchers - PhD holders according to
professional category.
11
Figure 3. Distribution of Member researchers - Non-PhD holders according to
professional category.
12
3. OBJECTIVES AND ACHIEVEMENTS
3.1 Unit description
CIMAR became an Associated Laboratory (CIMAR LA) in 2002 as a partnership between CIIMAR
(University of Porto) and CCMAR (University of Algarve). CIMAR LA is directed by a managing
board composed by six members, three from each of respective executive board of the two
partner institutions. The Director of CIIMAR is the head of the managing board and President of
CIMAR LA and the Vice-Vice-President is the Director of CCMAR. Board members meet regularly
for progress evaluation and strategic planning. CIIMAR and CCMAR are two separate legal
entities and management and implementation of administrative tasks must be carried out
separately. The leaders at the two centers are in permanent contact ensuring effective
management of CIMAR LA.
CIMAR LA integrates 28 research groups, scientifically organized in 4 research lines each with
two coordinators: 1) Ecology, Biodiversity and Management of Aquatic Ecosystems; 2)
Environmental Chemistry and Toxicology; 3) Biology and Marine Biotechnologies; 4)
Aquaculture. Two services interface operates within the LA, for analytical chemistry and
molecular and genetic research. In the last two years, a physical oceanography and coastal
dynamics and engineering has been established at CIMAR LA, adding interdisciplinarity and a
possible research line on ocean and coastal dynamics. This issue will be discussed within the
scientific board and external advisory committee.
CIMAR LA has five horizontal programmes: 1) Public awareness of science; 2) Post-gradute
studies; 3) Thematic networks; 4) Technology transfer; 5) Support to public policies.
A annual meeting of CIMAR LA allows the participation of all members from two distant regions,
facilitating communication and enable evaluation of progress and strategic planning. In 2009,
this meeting was not organized and it was transferred for the 1st semester of 2010. Members of
our external Advisory Board - composed of 5 scientific personalities – have participated in these
annual meetings when possible.
CIMAR LA has established formal institutional links with the Marine Geology department of
LNEG (former IGM), covering aspects related to climate change. This relationship will continue
and is progressively and more formally integrated as we develop a common strategy and
complementary of human resources. As a result of an already significant level of collaborative
work in research projects and post graduate student supervision in the areas of behavioral
endocrinology, evolutionary biology and conservation, CIMAR LA also established formal links
with the Research Unit on Eco-Ethology (UIEE) at the Higher Institute of Applied Ethology (ISPA)
and an application to FCT has been made for UIEE to become the third research unit of CIMAR
LA.
An agreement was made with CESAM-LA (University of Aveiro) in 2004 for developing and
implementing a large collaborative program that includes a Joint Doctoral Program in Marine
and Environmental Sciences (launched in 2007/2008), a coastal monitoring program and a
capacity developing program focusing on Portuguese speaking countries.
CIMAR LA network was also enlarged by regional agreements with several research institutions,
such as ISR – Institute of Systems and Robotics and INEGI – Institute for institute for mechanical
13
engineering and industrial management, in Porto. Two small research groups of CIMAR were
setup in Madeira and Azores Archipelagos.
3.2 General objectives
The main objectives of CIMAR LA are “to understand natural processes in the ocean and the
coastal zone, to study and implement sustainable exploitation of aquatic resources and the
impact of human activities in the environment”. With these objectives from the initial 10 lines of
research, 4 lines have consolidated: 1) Ecology, Biodiversity and Management of Aquatic
Ecosystems; 2) Environmental Chemistry and Toxicology; 3) Biology and Marine Biotechnology;
4) Aquaculture. With the recent recruitment of researchers in the area of physical oceanography
coastal dynamics and engineering one further line is being considered to be added, as previously
mentioned.
At a national level, CIMAR LA is a Centre that covers distinct biogeographical areas in Portugal,
including the Madeira and Azores Archipelagos, and therefore one of the objectives is to
monitor and compare the coastal environments in the two main regions of implementation
(North of Portugal and Algarve), both in the context of nature conservation, the impact of
human activities, and the sustainability of aquaculture and fisheries. The presence of research
groups in the Archipelagos, and the reinforcement of the oceanic and coastal dynamics research
group, open new opportunities to enlarge the CIMAR LA activities to the North-eastearn
Atlantic. After eight years of CIMAR LA activity, with a strong effort in international cooperation
and networking, the research groups are involved in international scientific projects and allow
CIMAR LA to become an actor in the ocean systems knowledge and the study of the effects of
global and climate changes. The Centre is also working hard at more fundamental biology, as
marine organisms are a source of models to answer fundamental questions and are a source of
molecules for many applications.
One of the strategic objectives of CIMAR LA is to extend scientific cooperation and training to
tropical and subtropical regions, especially Portuguese speaking countries.
Horizontal and cross-cutting objectives include: a) to intensify links with companies and
whenever possible promote innovation, technology transfer and entrepreneurship among its
members, b) to promote outreach/Science and Society activities through interaction with
schools, “Ciência Viva” centres, and the wider public; c) to promote networking activities among
the scientific communities and various stakeholders, d) to promote training through various
activities, including MSc and Doctoral programs, post-graduate courses and summers schools, e)
to support public policies.
3.3 Main achievements during the year of 2009
NUMBERS
Scientific projects raised 6% in budget in 2009 vs 2008. R&D contracts with industry increased
400% in budget in 2009 vs 2008. Networking actions raised 36% vs 2008.
14
Increase in output continued: compared to 2007 and 2008, 2009 saw a boost of ISI papers, in
44% and 20%, respectively. Intergroup papers raised (10% of sci. output).
RL1
Genome sequencing and studies of genetic variability of key species were made, with advances
on dependence of gene flow on both distance and habitat, with implications for ecosystem
disturbance recovery.
Advances in marine biodiversity existed, including new records and hypotheses testing on
biodiversity loss effects on the ecosystem functioning, at a pan-European scale. Progresses on
processes were made on estuarine fish assemblages, whereas integrative innovation was done
via a whole ecosystem (metabolism) approach of Ria Formosa.
Tools towards the implementation of the WFD were developed, along with ecotoxicity lab
bioassay, and microsatellite analysis software. New data was got on toxicity & detoxification.
RL2
New developments were made in: analytical chemistry; bioremediation; software for
evolutionary analyses; a bioassay linking behavior-biomarkers; biomarkers validation.
Characterizations of gene/protein evolution and functional diversification were made in GSTs,
RXR receptor, among others, offering evolutionary insights and mechanistic interactions. There
were progresses in processes of toxicity & detoxification.
Relationships among OMICS, biomarkers and population endpoints were made, inc. with
combined effects of global stressors, provided wider pictures of water quality and ecosystem
health. Global warming was tackled, relating it with nitrogen cycle novelties. Biodiversity was
studied in extreme environments, such transantarctic mountains.
RL3
New methods were set up for: subtractive hybridization and cDNA library production;
recombinant protein production from genes; isolation of fish embryonic stem cells.
A new research area was set up in chemical biology, encompassing characterization of bioactive
secondary metabolites and associated technology for their characterization.
A new bio-refinery strategy was set up for the production of High Value Products, at competitive
prices with petrodiesel.
Development and refinement of program GROMACS for classical protein dynamics existed,
while revealing kinetic mechanisms for protein folding.
The impact of cell Ca availability through environmental challenges resulted in the largest public
gill and teleost skin transcriptome.
15
A major chemical survey for xenoestrogenic endocrine disrupting compounds in Portugal water
ways was made in parallel with the development of methods, endpoint analysis and use of
biomarkers of disease- impact of toxicants in fish and mollusks.
RL4
Feeding practices and dietary formulations were optimized for new aquaculture species,
whereas increasing nutritional knowledge for established marine species. The efforts included
advances in comparative evaluation among species and establishing the potential for
aquaculture of promising omnivorous fish species. Info on nutritional requirements, food
utilization and behavior of ornamental fish were generated.
Innovative advances were made to supersede the bottleneck of Octopus culture.
It was achieved a successful pilot-scale testing of an eco-friendly practical diet (low fishmeal
levels, reduced soluble phosphorus losses) for gilthead sea bream.
The above was tied with new strategies of valorization of aquaculture products, and fish
immunostimulation strategies to enhance stress resistance and immune response.
16
4. ACTIVITIES
4.1 Integrative/multidisciplinary activities during the year of 2009
A significant effort was made to promote Integrative/multidisciplinary activities in CIMAR LA,
including the following example:
-
Data basing of ecosystem data (M@RBYS): CIMAR initiated and made a significant
contribution to integrate in a single database existing information on data from coastal
marine and marine ecosystems with a view of connecting to databasing infrastructures
in Europe such as Lifewatch and others. This database is now maintained by the
Estrutura de Missão para a Extensão da Plataforma Continental.
-
Long term ecosystem research (LTER): CIMAR researchers led applications for the
establishment of LTER sites in the north and south of Portugal. The plan involves
multiple disciplines and approaches (geology, ecology, metagenomics, etc).
-
Ocean tracking network: Bottom morphology and topography of the Gibraltar straight
has been completed to establish the best places to position acoustic receivers.
-
Partnership with INEGI (Insitute of Mechanical Engineering and Industrial Management)
for continued development of hyperbaric chambers involving teams of engineers and
marine biologists.
-
Collaboration between engineers and biologists was also responsible for the
development of a biosensor to measure the effect of pollutants on aquatic animals
(patent pending).
-
Hire of new researchers in complementary areas of coastal engineering and physical
oceanography to broad multidisciplinarity.
-
Climate change and human migrations – multidisciplinary research correlating past
climate with population movements involving anthropologists, geologists and biologists.
-
Collaboration between researchers from physical oceanography and genetics to study
the dispersal of organisms across the Atlantic.
-
Coastal Monitoring programs integrate physical oceanographers, remote sensing
researchers, geologists, biologists and engineers. Some of those projects aim at ocean
and atmospheric forecast, the study of sedimentary transport, and the changes in
marine coastal communities.
-
Doctoral Porgramme in Marine and Environmental Sciences involves CIMAR and CESAM
(Universities of Porto, Aveiro and Algarve). A joint European Master Erasmus Mundus on
Marine Biodiversity and conservation attracts students from around the world which
have voted for CCMAR-CIMAR LA as their preferred host institution.
-
CIMAR is founding member of the R&D&I consortium in Ocean Science and Technology Consortium OCEANOS, linking five state laboratories (INRB/IPIMAR, IH, IM, LNEC and
17
LNEG), four universities and one research network (IMAR). The proposal has been
accepted by the Minister of Science, Technology and Higher Education in 2009, with a
funding envelop for 10 years. The science plan includes five thematic areas: Ocean
Dynamics and Climate, Seafloor Dynamics, Marine Biogeochemistry, Ecosystem Function
and Dynamics, and Marine Resource Management and Spatial Planning. In addition to
the main thematic areas, three transversal themes are considered: Operational
Oceanography, Ocean Data Management, and Marine Technologies and Infrastructures.
4.2 Outreach activities during the year of 2009
CIMAR has performed a significant effort in the promotion of outreach/Science and Society
activities, to foster the dialogue between scientists and society, to improve the public
knowledge and perception of science and to increase the interest of the young students towards
scientific careers.
CIMAR is directly managing CMIA-Vila do Conde, through a protocol with the municipality
(www.cmia-viladoconde.net). The activities promoted during 2009 included several temporary
exhibitions, conferences and professional courses and regular in house and field activities for
public of different ages, with a particular emphasis on young students (3400 visitors, 52
schools/institutions). Moreover, two public aquariums and fisheries museums have been
managed by CIMAR researchers: Aguda Littoral Station - ELA (www.fundacao-ela.pt, ca 50000
visitors/year) and River Minho AQUAMuseum (www.cimsoft.pt/aquamuseu, ca 30000
visitors/year). Both stations have a special relevance in what concerns Environmental Education
Programs.
Since 2006, CIMAR has been organizing the Sea Itinerant University (UIM)
(http://www.cimar.org/CIIMAR/en/hz_pas_uim.htm), a program that aims to promote the
maritime identity of Portuguese and Spanish students, through the contact with the sea and its
potentialities, related activities and culture. Courses are organised in land and on board the
vessel CREOULA, a 4-masted training ship of the Portuguese Navy built in 1937. The theme of
the 2009 course was "Atlantic, an open frontier".
CIMAR regularly participates in the Ciência Viva Programme (http://www.cienciaviva.pt), to
contribute to the promotion of a scientific and technological culture among the Portuguese
population and more specifically to encourage the use of experimental methods in science
learning, involving both scientific and school communities. In 2009, CIMAR participated in the
following activities: Scientists go to School, Science on Holidays for Young People, Biology
Activities in the Summer, Exploring the Ocean at School for the implementation of the Ocean
Exploration in the International Education Community programme (Explore) of NOAA (USA).
In 2009, CIMAR continue the collaboration with the Visionarium - Europarque Science Centre to
implement a national project using Daphnia as biological model for experimental teaching of
sciences
in
high
schools
(220
schools,
320
teachers,
2500
students).
(http://projectodaphnia0.blogs.sapo.pt/).
18
CIMAR
is
also
involved
in
MOBIDIC
School
in
the
Beach
(http://www.estgm.ipb.pt/~projig/mobidic), an outreach program for intertidal biodiversity
monitoring in beaches of the North coast (12 high schools, 400 students).
CIMAR regularly participates in other events and exhibitions to encourage high schools students
to engage in scientific careers, such as the “Junior University” (http://universidadejunior.up.pt/,
30 Aug-4 Sept), the “Science, Education and Innovation” exhibition by the University of Porto
(26-29 March), and the LAB-IT®-The Itinerant Laboratory in Molecular Genetis. Moreover,
CIMAR maintains its participation in the EXPOMAR exhibition (29 April-3 May) in Algarve,
organize seminars to the general public and encourage the participation of volunteers within the
scope of scientific projects.
CIMAR activities were disseminated through the media, namely in non-scientific journals and
newspapers (e.g. CORDIS News, National Geographic, Visão), and participation in TV and radio
programs.
19
5. FUNDING
Origin
2005
2006
2007
2008
2009
LA FCT *
1.536.008,00
1.092.380,00
2.913.911,00
2.977.162,00
2.788.243,44
Units FCT **
0,00
0,00
0,00
0,00
0,00
Project FCT
552.605,00
570.952,00
827.775,00
2.020.917,77
2.096.417,55
Other
National
108.507,00
197.458,00
240.018,00
487.715,32
928.168,44
Other
International
704.412,00
659.879,00
1.082.379,00
1.200.367,88
1.018.067,73
National
Industry
110.233,00
140.634,00
258.989,00
140.135,50
464.280,75
International
Industry
0,00
0,00
0,00
0,00
88.421,10
Total
3.011.765,00
2.661.303,00
5.323.072,00
6.826.300,47
7.383.599,01
(*) Base + Programmatic; (**) Base + Programmatic of the units before the criation of the LA.
20
6. GENERAL INDICATORS
6.1 Composition and training
2005
2006
2007
2008
2009
Total
No. of researchers proposed
6
5
0
12
15
38
No. of researchers hired (LA)
9
4
2
14
6
35
Balance
3
-1
2
2
0
4
No. of Researchers Hired
(Ciência Programme)
0
0
0
10
6
16
154
199
203
No. of researchers (FTE)
Training Masters (Master
theses completed)
25
17
44
57
70
213
Training PhDs (PhD theses
completed)
8
23
37
26
24
118
21
6.2 Researchers hired
Name
Start date
End date
Alexandre Marnoto de Oliveira Campos
16-02-2009
15-02-2012
Elsa Maria Branco Froufe Andrade
01-12-2009
30-11-2012
Fernando José Tuya Cortés
01-06-2009
31-05-2012
Iacopo Bertocci
01-07-2009
30-06-2012
Kim Richard Larsen
01-07-2009
30-06-2012
Marta Mega Rufino
16-02-2009
15-02-2012
Name
Start date
End date
Paula Raquel da Silva Jorge Coutinho Ferreira
07-01-2009
06-01-2012
6.3 Technical personal hired under LA contract
6.4 Additional comments
The difference between the No. of Researchers Proposed (15) and the No. of Researcher Hired
in the Ciência Programme (6) was mainly related to the fact that some of the open vacancies
were closed without any applicant with the required profile and FCT was not able to allow us to
re-open the vacancies.
22
7. RESEARCH LINES (THEMATIC AREAS)
RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems
7a. General description
1. Designation
Ecology, Biodiversity and Management of Aquatic Ecosystems
2. Principal investigator
Karim Erzini
3. Main scientific domain
Marine Sciences
7b. Objectives and achievements
1. General objectives
The general objectives of RL1 are to study selected aspects of marine geology, oceanography,
marine biodiversity and ecosystems, integrating research to provide a sound scientific basis for
assessment, management, and policy related with the marine ecosystems. Within this
framework, the objectives include the following:
-
Promote the geological research of the oceans and coastal regions, the
paleoceanography, and the processes related to the occurrence of marine mineral
resources;
-
Determine the composition, abundance and distribution of the national marine
biodiversity;
-
Determine the impacts of climate change and local human action by monitoring and
studying coastal and estuarine ecosystem functioning, and long-term changes in marine
communities and habitats;
-
Study the genetics, population biology, ecology and recruitment of ecologically and
commercially important species;
-
Investigate the physiological adaptations of migratory fishes, to different salinity and
hydrostatic pressure;
-
Participate in International networks on marine biodiversity;
23
-
Train young scientists and to disseminate scientific knowledge.
2. Main achievements
Ecotoxicology and pollution
-
Development of ecotoxicity lab bioassay and determination the physiological impact of
toxicant exposure and environmental changes through the measurement of biomarkers.
Study of effects of PAHs, pesticides, metals and pharmaceuticals on several marine and
freshwater species of temperate and tropical areas, including mechanisms of toxicity
and detoxification.
Genetics and phylogeography
-
Sequencing of genomes of marine snails and algae. Study of genetic variability in a
variety of species, including research on the dependence of gene flow on both distance
and habitat continuity (landscape effects) and implications for recovery from ecosystem
disturbance.
Estuarine and lagoon processes
-
Research on estuarine fish assemblages: recruitment, habitat use, long-term monitoring,
and on invasive species. Assessment of the ecological status of transitional waters and
development of tools towards the implementation of the WFD. Quantification of the
whole ecosystem metabolism of the Ria Formosa lagoon, and of the role of seagrass,
saltmarsh and green algae communities on carbon and nitrogen uptake.
Global changes
-
Publication of the highest resolution sedimentary record of the Holocene, allowing
detailed study of the sea level rise. Study of the effects of marine acidification on
bivalves. Discovery of the occurrence of a novel inhibitory interaction between organic
sulfur degradation compounds and the last step of denitrification in estuarine
sediments.
Technical developments
-
Software to analyse microsatellite allele binning and microsatellite genetic markers for a
variety of species.
Biodiversity
-
Important contributions to the knowledge of the marine biodiversity and its distribution
and abundance, including many new records. Test explicit hypotheses on the effects of
loss of biodiversity on ecosystem functioning at a pan-European scale.
Networks
-
24
Participation in several EU Networks (e.g. MARBEF, MARS, EMBRC, EPBRS).
Research training
-
Training at the undergraduate and graduate levels (MSc and PhD programs),
participation in the Erasmus Mundus MSc in Marine Biodiversity and Conservation.
Dissemination & Outreach
-
Activities to foster dialogue between scientists and society (e.g. talks, school visits,
summer prog).
7c. Research lines output
1. Collaborative publications in peer review journals (If more that one Research Group of
the LA is involved. Title and full citation in original language)
Examples of collaborative publications:
1. Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, AA,Vasconcelos, MTSD. 2009. Study of the
influence of different organic pollutants on Cu accumulation by Halimione portulacoides.
Estuarine Coastal and Shelf Science 85: 627-632.
2. Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, AA,Vasconcelos, MTSD. 2009. Influence of
surfactants on the Cu phytoremediation potential of a salt marsh plant. Chemosphere 75: 135140.
3. Araújo, R, Bárbara, I, Tibaldo, M, Berecibar, E, Díaz Tapia, P, Pereira, R, Santos, R, Sousa Pinto,
I. 2009. Checklist of benthic marine algae and cyanobacteria of Northern Portugal. Botanica
Marina 52: 24-46.
4. Assis, J, Tavares, D, Tavares, JT, Cunha, AH, Alberto, FA, Serrão, EA. 2009. Findkelp, a GISbased community participation project to assess Portuguese kelp conservation status. Journal of
Coastal Research 56:1469-1473.
5. Cunha, AH, Assis, J, Serrão, E. 2009. Estimation of available seagrass meadow area in Portugal
for transplanting purposes. Journal of Coastal Research SI 56: 1100-1104.
6. Ferreira, F, Santos, MM, Castro, LF, Reis-Henriques, MA, Lima, D, Vieira, MN, Monteiro, NM.
2009. Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: a new sentinel
species for estrogenic chemical pollution monitoring in the European Atlantic coast?
Comparative Biochemistry Physiology C 149: 58-64.
7. Francisco, SM, Castilho, R, Soares, M, Congiu, L, Brito, A, Vieira, MN, Almada, VC. 2009.
Phylogeography and demographic history of Atherina presbyter (Pisces: Atherinidae) in the
North-eastern Atlantic based on mitochondrial DNA. Marine Biology 156(7): 141-1431.
25
8. Freitas, V, Cardoso, JFMF, Santos, S, Campos, J, Drent, J, Saraiva, S, Witte, JIJ, Kooijman, SALM,
van der Veer, HW. 2009. Reconstruction of food conditions for Northeast Atlantic bivalve
species based on Dynamic Energy Budgets. Journal of Sea Research 62:75-82.
9. Freitas, V, Costa-Dias, S, Campos, J, Bio, A, Santos, P, Antunes, C. 2009. Patterns in abundance
and distribution of juvenile flounder, Platichthys flesus, in Minho estuary (NW Iberian
Peninsula). Aquatic Ecology 43: 1143-1153.
10. Le Corguille, G, Pearson, GA, Valente, M, Viegas, C, Gschloessl, B, Corre, E, Bailly, X, Peters, A,
Jubin, C, Vacherie, B, Cock, M, Leblanc, C. 2009. Chloroplast genomes of two brown algae,
Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal
derived plastids. BMC Evolution Biology 9: 253.
11. Monteiro, C, Engelen, AH, Serrão, EA, Santos, R. 2009. Habitat differences in the timing and
reproduction of the invasive alga Sargassum muticum (Phaeophyta, Sragassaceae) over tidal and
lunar cycles. Journal of Phycology 45: 1-7.
12. Rubal, M, Guilhermino, L, Medina, MH. 2009. Individual, population and community level
effects of subtle anthropogenic contamination in estuarine meiobenthos. Environmental
Pollution 157: 2751-2858.
2. Collaborative other publications (If more than one group is involved. Books, chapters or
full papers published in conference proceedings. Title and full citation in original
language)
1. Almeida, CMR, Mucha, AP, Carvalho, PN, Basto, MCP, Vasconcelos, MTSD. 2009. Mutual
interactions between roots of salt marsh plants and sediments and their relevance for toxicity
endpoints and rhizoremediation. In: Greig Ramsey and Seoras McHugh (Eds). River Sediments,
Frank Columbus Ed., Nova Science Publishers, ISBN: 978-1-60741-437-7.
2. Dias, S, Sousa, R., Lobón-Cerviá, J, Laffaille, P. 2009. The decline of diadromous fish in Western
European inland waters: main causes and consequences. In: McManus, NF, Bellinghouse, DS.
(Eds). Fisheries: Management, Economics and Perspectives, pp. 67 - 92. Nova Science Publishers,
Inc., New York, USA.
3. Master and PhD theses completed (Co-supervision or clearly multidisciplinary projects)
MASTER THESES
From the Master theses completed in 2009, 16 were in collaboration: with different RG within
or outside the RL (4), with national (8) and international (4) institutions.
PhD THESES
From the PhD theses completed in 2009, 11 were in collaboration with RG within the RL (1), with
national (4) and international (6) institutions.
26
Alquicira, EFR. 2009. Filogeografia de algas Corallinales (Rhodophyta) das Califórnias. PhD thesis,
University of Azores. Supervisor: AI Neto (CIMAR-Biodiversity of Aquatic Ecosystems), Cosupervisor: R Rodríguez (Univ Baja Califórnia Sur, México).
Amaral, AM. 2009. Aquaculture impact of clam Ruditapes decussatus in the Ria Formosa: effects
on the ecosystems and species physiology. Univ Santiago Compostela, Spain. Supervisor: L
Chicharo (CIMAR-ECOREACH).
Campos, JCVB. 2009. The eco-geography of the brown shrimp Crangon crangon in Europe. Vrije
Universiteit Amsterdam, Netherlands. Supervisor: SALM Kooijman, Co-supervisor: H van der
Veer (NIOZ).
Carvalho, L. 2009. Metodologias para a avaliação integrada dos impactos cumulativos em
sistemas fluviais de pequenas bacias sujeitas a elevadas pressões antropogénicas. PhD thesis in
Aquatic Sciences, ICBAS, University of Porto. Supervisor: A Bordalo e Sá (CIMAR-Hydrobiology),
Co-Supervisors: R Cortes (UTAD), JP Martins (Fac Engineering, Univ Porto).
Cisneros, KL. 2009. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais. PhD
thesis, University of Azores. Supervisor: AI Neto (CIMAR- Biodiversity of Aquatic Ecosystems),
Co-supervisor: R Riosmena Rodríguez (Univ Baja Califórnia Sur, México).
Leitão, FMS. 2009. Algarve Artificial reefs colonization processes and fish trophic ecology:
implications for the local near shore fisheries. PhD thesis, University of Algarve. Supervisor: K
Erzini (CIMAR-FBC), Co-supervisor: MN dos Santos (IPIMAR).
Marçalo, ALB. 2009. Sardine (Sardina pilchardus) delayed mortality associated with purse seine
slipping: contributing stressors and responses. PhD thesis, University of Algarve. Supervisor: K
Erzini (CIMAR-FBC), Co-supervisor: Y Stratoudakis (IPIMAR).
Martins, R. 2009. Is DAX-1 a sex-determining gene in fish? PhD thesis in Aquatic Sciences, ICBAS,
University of Porto. Supervisor: AVM Canario (CIMAR-CME), Co-supervisor: J Coimbra (CIMAREcopysiology).
Moreira de Sousa, JJ. 2009. Potential of integrating psi methodologies in the detection of surface
deformation. PhD thesis, University of Porto. Supervisor: L Bastos (CIMAR-OCD), Co-supervisor:
R Hanssen (TUD, Netherlands).
Saraiva, J. 2009. Inter-populational variation of reproductive behaviour in Salaria pavo. PhD
thesis, ICBAS, University of Porto. Supervisor: R Oliveira (ISPA), Co-supervisors: AVM Canario
(CIMAR-CME), J Coimbra (CIMAR-Ecophysiology).
Vieira, LMR. 2009. Methodologies for a sustainable development of estuarine ecosystems. PhD
thesis, University of Aveiro. Supervisor: L Guilhermino (CIMAR-ECOTOX), Co-supervisor: F
Morgado (CESAM, Univ Aveiro).
27
7d. Future research
1. Other information (If more that one group is involved. Patents/prototypes, organization
of conferences, industry contract research)
Members of the RL1 were involved in the organization of both National and International
conferences, industry and other Government/Organization contract research and networking
during 2009; only those actions which involved different groups from the RL1 or other RL in
CIMAR or of an interdisciplinary character are indicated.
Organization of conferences/workshops
-
BIOLIEF - World Conference on biological invasions and ecosystem functioning, 27-30
Oct, Porto, Portugal. http://www.ciimar.up.pt/biolief/
-
MarBEF Workshop on a European Monitoring Network 2009, 13-14 March, Porto,
Portugal.
-
JOCLAD 2009 (Classification and Data Analysis Workshop), 2-4 April, Faro, Portugal.
-
Workshop River Douro Estuary Management Plan, 23 Nov, Porto, Portugal.
-
Faro Ocean Tracking Network (OTN), 3-5 Dec, Faro, Portugal.
-
Workshop to implement two seagrass monitoring sites in Ria Formosa lagoon, included
in the world-wide SeagrassNet monitoring program (www.seagrassnet.org), Faro,
Portugal.
Conferences/workshops programmed for 2010
-
12º Encontro Nacional de Ecologia, Oct 2010, Porto, Portugal.
Industry and other Government/Organization contract research
-
Cartography and characterization of the marine communities off the National
Underwater Ecological Reserve between the Arade river and Ponta da Piedade (RENSUB
IV). Funded by Algarve River Basin Administration (Administração da Região Hidrográfica
do Algarve - ARH Algarve).
-
The BIOMARES LIFE project. Partly funded (50%) by SECIL, the cement manufacturer.
-
NATURA-MINHO-MIÑO, a project funded by Interreg IV POCTEP, the Territorial
cooperation program between North of Portugal and Galicia. This project aims the join
valorization of natural resources in the river Minho Hydrographical basin, the promotion
of sustainable development and biodiversity and habitat conservation (Natura 2000) in
the trans-boundary territory of North of Portugal and Galicia. It involves the
participation of regional authorities (General Directorate of Nature Conservation in
Galicia and ICBN - Institute for Nature Conservation and Biodiversity of the Portuguese
Ministry for the Environment) assuring the effective involvement in public policy advice.
28
-
Ecological status of coastal and transitional waters of the Portuguese coast -EEMA.
Funded by the Portuguese Water Institute, Ministry of Environment (INAG) for the
implementation of the Water Framework Directive.
-
National plan for the conservation of lamprey (Lampetra fluviatilis and Lampetra
planeri). Funded by EDP, energy sector company, Biodiversity fund.
-
River Douro estuary management plan. Funded by Northern River Basin Administration
(ARH Norte).
Networking
-
DivMar - National Network on Costal Marine Biodiversity (www.cimar.org/divmar). This
network is jointly coordinated by CIMAR (several research groups), CESAM and ISPA;
started in 2008 focusing their activities in 4 areas of the continental portuguese coast:
North of Portugal, Ria de Aveiro, Parque Natural da Arrábida, Ria Formosa.
-
MARBEF - Marine Biodiversity and Ecosystem Function Network of Excellence
(www.marbef.org); several CIMAR research groups participate in this platform that aims
to integrate and disseminate knowledge and expertise on marine biodiversity, with links
to researchers, industry, stakeholders and the general public.
-
MARS - European Network of Marine Institutes and Stations (www.marsnetwork.org),
EMBRC - European Marine Biological Research Centres (www.embrc.eu). The
participation in both networks provide opportunities for groups in CIMAR to join
activities that facilitate collaborations and shared access to infrastructures and to a
comprehensive set of coastal marine ecosystems and marine organisms.
-
EPBRS (www.epbrs.org), and the supportive networks BioPlatform (CIMAR coordination,
www.bioplatform.info) and Biostrat.
-
Seagrass Net Monitoring Program (www.seagrassnet.org).
Several groups participated in the meetings promoted by the North Regional Coordination and
Development Commission (CCDR-N), contributing to the elaboration of regional priorities for the
sustainable development of marine research in the North of Portugal and Galiza region that
resulted in a final report “Regional Agenda for the Sea”, published in February 2009. This action
will play an essential role in defining research priorities in the area of Marine research which will
benefit all CIMAR members.
A significant effort is being made to reinforce the cooperation between groups within the RL and
stimulating multidisciplinary research with other RL.
2. Future plans
Future plans are largely based on the continuation of the long-term research objectives outlined
above:
29
-
Contribute with relevant data and knowledge to the implementation of environmental
and marine policies e.g. the EU Water Framework Directive (project EEMA) and the
Habitats Directive for the marine environment, as well as participating in the
development of methodologies for implementation of other EU Directives, as the
Marine Strategy Directive and ICZM.
-
Continue study different aspects of marine biodiversity and its role on coastal aquatic
ecosystems, and to promote the sustainable use of marine resources through producing
knowledge that can assist in coastal and resource management and effectively
conveying this knowledge to the relevant managers and policy makers.
-
Contribute to the study of global changes through research on coastal erosion,
biodiversity and habitats to study the effects of global environmental changes and local
anthropogenic effects, including the impacts of alien species on coastal ecosystems, and
effects of marine acidification. Study the species persistence under climate variability,
using a variety of approaches.
-
Develop and implement hydrodynamic and biogeochemical models, and decision
support systems (DSS), to integrate knowledge about coastal ecosystems and to be used
as management tools.
-
Continue work on restoration of biodiversity and habitats and evaluation of the benefits
of marine protected areas, with particular emphasis on fish larval trophodynamics,
production, population dynamics and ecology, and spatio-temporal dynamics.
-
Assess toxicity effects of wastewaters using different test organisms and develop and
apply eco-technology techniques for improvement of water quality.
-
Continue the integration of the groups, increasing the synergies between present and
the new researchers that are being hired, thereby reinforcing existing lines of research,
and developing new research lines that will greatly improve our collective research
capacity and the response to societal needs.
-
Consolidate international cooperation, including participation in innovative educational
programs at the European level such as the. Erasmus Mundus MSc in Marine
Biodiversity and Conservation, and new PhD programs.
30
RL2 - Environmental Chemistry and Toxicology
1. Designation
Environmental Chemistry and Toxicology
Lúcia Maria das Candeias Guilhermino
Environment
The central objective of RL2 is to investigate the effects of natural and anthropogenic stressors
on aquatic ecosystems using a molecular to ecosystem level approach. Special attention has
been given to long-term alterations induced on marine ecosystems by global stressors
(pollution, invasive species and climate changes). Specifically, we intend to:
-
Develop methods to diagnose the presence and characterize stressors;
-
Study the interaction of stressors with molecular targets to investigate mechanisms of
toxicity and defenses;
-
Compare responses among species and establish phylogeographic patterns of evolution;
-
Investigate reflexes of molecular interactions at higher levels of biological organization;
-
Develop new methods for ecological risk assessment;
-
Study the functioning of ecosystems under biological and abiotic stress in a climate
changing world;
-
Monitoring water quality and developing strategies to improve it;
-
Develop methods for conservation, bioremediation and restoration.
-
80 publications in indexed journals by the core groups.
31
-
Optimization of analytical methods for quantification of anthropogenic chemicals, and
compounds released by vascular plants, bacteria, cyanobacteria, cyanotoxins.
-
Development of biological processes for bioremediation of soils and coastal areas.
-
Isolation and structural characterization of new allelopathic substances; cyanotoxins
profiles of several strains; phylogenetic analysis of worldwide strains of C. raciborskii.
-
Phylogenetic analyses on the evolution and functional diversification of cytosolic GSTs
and PhosphoProtein Phosphatases among metazoans and their molecular interaction
with toxins.
-
Characterization of the gene/protein evolution of the Retinoid X Receptor and the
matrix extracellular phosphoglycoprotein.
-
Development of software for evolutionary analyses on gene/protein sequences.
-
1 new bioassay linking behaviour with biomarkers; knowledge on relationships among
OMICS, biomarkers and population endpoints.
-
Validation of several biomarkers for use in biomonitoring studies (temperate and
tropical ecosystems).
-
Knowledge on water quality, chemical contamination and ecosystem health (national,
tropics), and effects of pollution on several native organisms.
-
Knowledge on acute and chronic effects of EDCs on embryos and on the retinoic acid
signaling pathway of fish.
-
Isolation of several nuclear receptors in invertebrates and study of their interaction with
environmental contaminants.
-
Cloning and sequencing of ABC transporter genes in fish.
-
Knowledge on the mechanisms of toxicity and detoxication of several environmental
contaminants in invertebrates and fish.
-
Knowledge on the nitrogen cycle in relation to global warming.
-
Discovery of a novel inhibitory interaction between organic sulfur degradation
compounds and the last step of denitrification.
-
Research on bacteria, cyanobacteria and ammonia oxidizers biodiversity and response
to environmental constraints in Transantarctic Mountains.
-
Knowledge on the combined effects of global stressors (pollution, invasive species,
climate changes) on national ecosystems.
-
Knowledge on water quality, its relation with human diseases and strategies for water
quality improvement in the tropics.
32
1. Collaborative publications in peer review journals (If more that one Research Group of
Examples of collaborative publication:
Estuarine Coastal and Shelf Science 85: 627-632.
surfactants on the Cu phytoremediation potential of a salt marsh plant. Chemosphere 75: 135140.
3. Baptista, MS, Stoichev, T, Basto, MCP, Vasconcelos, VM, Vasconcelos, MTSD. 2009. Fate and
effects of octylphenol in a Microcystis aeruginosa culture medium. Aquatic Toxicology 92: 59-64.
4. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Oudra, B, Del Campo, FF, Vasconcelos, V.
2009. Compensatory growth induced in zebrafish larvae after pre-exposure to a Microcystis
aeruginosa natural bloom extract. International Journal of Molecular Sciences 10(1): 133-146.
Comparative Biochemistry and Physiology C 149: 58-64.
6. Leão, PN, Vasconcelos, MTSD, Vasconcelos, V. 2009. Allelopathic activity of cyanobacteria on
green microalgae at low cell densities. European Journal of Phycology 44: 347-355.
7. Osswald, J, Carvalho, AP, Claro, J, Vasconcelos, V. 2009. Effects of cyanobacterial extracts
containing anatoxin-a and of pure anatoxin-a on early developmental stages of carp.
Ecotoxicology and Environmental Safety 72:473-478.
8. Osswald, J, Rellan, S, Gago, A, Vasconcelos, VM. 2009. Production of anatoxin-a by
cyanobacterial strains isolated from Portuguese fresh water systems. Ecotoxicology 18: 11101115.
9. Reis, PA, Antunes, JC, Almeida, CMR. 2009. Metal levels in sediments from the Minho estuary
salt marsh: a metal clean area? Environmental Monitoring and Assessment 159: 191-205.
10. Rellan, S, Osswald, J, Saker, M, Gago, A, Vasconcelos VM. 2009. First detection of anatoxin-a
in human and animal dietary supplements containing cyanobacteria (blue-green algae). Food
and Chemical Toxicology 47: 2189-2195.
Pollution 157: 2751-2858.
33
12. Santos, MM, Eenes, P, Reis-Henriques, MA, Kubala, J, Castro, L, Filipe, C, Vieira, M.N. 2009.
Organotin levels in seafood from Portuguese markets and the risk for consumers. Chemosphere
75: 661-666.
13. Soares, J, Coimbra, AM, Reis-Henriques, MA, Monteiro, NM, Vieira, MN, Oliveira, JMA,
Guedes-Dias, P, Fontainhas-Fernandes, A, Parra, SS, Carvalho, AP, Castro, LF, Santos, MM. 2009.
Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental
exposure to low levels of ethinylestradiol. Aquatic Toxicology 95: 330-338.
language)
MASTER THESES
From the Master theses completed in 2009, 13 were in collaboration with national (3) and
international (1) institutions. Ex. of collaborative theses:
Gomes, JMBC. 2009. Chemical characterization and influence of the bottling process of a natural
sparking mineral water. Master thesis, Faculty of Sciences, University of Porto. Supervisor: MC
Basto (CIMAR-CS&B), Rui Santos (Laboratório do INETI, S. Mamede de Infesta).
Laverco, P. 2009. Análise biológica de biofilmes em sistemas de transporte de águas de
consumo. Master thesis in Biology and Management of Water Quality, Faculty of Sciences,
University of Porto. Supervisor: V Vasconcelos (CIMAR-EDGE), Co-supervisor: J Poças Martins
(Faculty of Engineering, University of Porto).
Martins, MLX. 2009. Eco-efficiency: development of assessment tools. Master thesis, Faculty of
Sciences, University of Porto. Supervisor: MC Basto (CIMAR-CS&B), responsible from Ecoinside:
Joaquim Guedes.
Muntz, A. 2009. Linking different levels of biological organization after acute exposure of a
widely used pharmaceutical (diazepam) in Dicentrachus labrax). Master thesis in Écologie et
Développement Durable, Institute de Biologie et d´Ecologie Appliquée (IBEA), Université
Catholique d’Ouest, Angers, France. Supervisor: L Guilhermino (CIMAR-ECOTOX).
PhD THESES
From the PhD theses completed in 2009, 2 were in collaboration with national (2) institutions.
Ex. of collaborative theses:
34
Lima, IM. 2009. Ecotoxicological effects of petrochemical products on natural populations of
Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal. PhD thesis in
Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Guilhermino (CIMAR-ECOTOX), Cosupervisor: AMVM Soares (CESAM, University of Aveiro).
thesis in Biology, University of Aveiro. Supervisor: L Guilhermino (CIMAR-ECOTOX), Cosupervisor: F Morgado (CESAM, University of Aveiro).
7d. Future research
conferences, patents/prototypes, industry and other Government/Organization contract
research and networking during 2009; only those actions which involved different groups from
the RL2 or other RL in CIMAR or of an interdisciplinary character are indicated.
Organization of conferences/workshops:
-
1st Iberian Congress in Cyanotoxins,
http://www.cimar.org/cic2009/
6-7
July
2009,
Porto,
Portugal.
-
7th AIEC Congress, Iberian Association of Comparative Endocrinology, 6-9 September
2009, Porto, Portugal. http://www.ciimar.up.pt/7aiec/index.html
-
October, Porto, Portugal. http://www.ciimar.up.pt/biolief/
-
Workshop: Plano de Ordenamento do Estuário do Rio Douro, 23 November, Porto,
Portugal.
Conferences/workshops programmed for 2010:
-
Workshop New Tools for better planning, response management and damage
assessment in HNS, inert and oil spills, 30 April 2010, European Agency for Maritime
Safety (EMSA), Lisbon, Portugal.
Industry and other Government/Organization contract research:
-
Ecological status of coastal and transitional waters of the Portuguese coast -EEMA.
Funded by the Portuguese Water Institute, Ministry of Environment (INAG) for the
implementation of the Water Framework Directive.
-
River Douro estuary management plan. Funded by Northern River Basin Administration
(Administração da Região Hidrográfica do Norte - ARH Norte).
35
-
Survey of Some Pharmaceutics Effects on Freshwater Cyanobacteria. Does Good
Manufacturing Practice (GMP) during pharmaceutics manufacture Influence the
Biological Response? Funded by Hovione FarmaCiencia S.A.
-
ARCOPOL - The Atlantic Regions Coastal Pollution Response, a project funded by
Interreg IV Atlantic Area, the transnational territorial cooperation program between
regions of the Atlantic Area. This project aims to improve the preparedness, response
and mitigation capabilities of local responders to accidental coastal pollution specifically
against oil, hazardous and noxious substances and inert spills. It involves the
participation of regional and national authorities (National Maritime Authority of the
Ministry of National Defence and Port Authority in Portugal) assuring the effective
involvement in public policy advice.
Networking actions:
-
ICES Working Group on Biological Effects of Environmental Contaminants. www.ices.dk
-
Red Iberoamericana de Toxicología y Seguridad Química. http://ritsq.org
Several groups participated in the meetings promoted by the North Regional Coordination and
sustainable development of marine research in the North of Portugal and Galiza region that
A significant effort is being made to reinforce the cooperation between groups within the RL and
stimulating multidisciplinary research with other RL.
2. Future plans
Groups in RL2 will continue to investigate according the central and specific objectives indicated
in the section Objectives of this report. In the next years, special attention will be given to the
investigation of:
-
Analytical tools to measure residuals of stressors in biotic and abiotic compartments;
-
Vascular plants for rhizoremediation and role of exudates in biogeochemical
phenomena;
-
Mechanisms of toxicity and detoxication of anthropogenic and natural stressors (e.g.
interaction with transporters, peroxisomes, nuclear receptors, and other molecules,
genetic alterations, endocrine disruptor effects)
-
Validation of toxicogenomics, toxicoproteomics and toximetabolomics tools;
-
Phylogeographic relationships and evolution in relation to stress (toxins, pollution,
climate changes) exposure and responses;
36
-
Understanding how the presence of chemical pollution drives the evolution of
populations of key estuarine species;
-
Effects of pollution and natural stressors on ecosystem functioning, including
interspecies relationships;
-
Development of methodologies for risk assessment of natural and antropogenic
stressors (e.g. oil and chemical spills, pharmaceuticals, pesticides, bacteria and
cyanobacteria toxins)
-
Effects of global changes on the evolution and functioning of ecosystems under stress;
-
Interactions between organic sulfur degradation compounds and the last step of
denitrification, including processes (involved in global warming) and microbial
communities;
-
Effects of global stressors in extreme environments;
-
Methods for ecosystem management and restoration, water quality improvement and
human diseases control (cholera, chemical exposure) for tropical, sub-tropical and
temperate regions;
-
Environmental exposure to chemical and biological agents and public health, especially
risk groups (e.g. children);
-
Knowledge, tools and strategies for conservation, remediation and restoration to
support scientific based policies (national, EU and global level).
37
RL3 - Biology and Marine Biotechnologies
1. Designation
Biology and Marine Biotechnologies
Deborah Mary Power
Biological Sciences
The Biology and Marine Biotechnology research line was consolidate in 2009 with the inclusion
of a new research group and area. The central pillars are the same as previous years:
-
To extend fundamental knowledge about marine organisms at a molecular to whole
organism level and across the evolutionary scale;
-
To establish how natural and man-made environmental challenges impact organismal
function;
-
To reinforce the drive to promote the identification of potential “spin-offs” arising from
basic biology and stimulate pilot studies and links with industry (eg. “near market”
solutions, such as, genotyping for aquaculture species, diagnostic tests in pathology).
There is continued commitment of the research line to disseminate knowledge in peer-review
journals of good impact and at International conferences. A newly ratified objective is to provide
high quality training for a new generation of scientists and contribute to generate a “knowledgebased society”. Translation of research activities and progress for communication to other
stakeholders (scientists, policy makers, industry, teachers and the public) remains an important
objective.
This RL includes 8 research groups and this section will present only major achievements, those
perceived as having a significant impact at a national or international level or represent
technological advances. Specific research progress is indicated in individual group reports.
-
38
Development of a new research area in chemical biology encompassing characterization
of bioactive secondary metabolites produced by terrestrial and marine derived macro-
and microorganisms and associated technology for their chemical and biological
characterization.
-
Implementation of a bio-refinery strategy for the production of High Value Products
(HVPs) at competitive prices with petrodiesel. Several microalgal strains have been
isolated which are fast-growing and have a polyunsaturated fatty acid profile suitable
for the production of bio-jetfuel.
-
The impact of changed calcium availability through natural or man-made environmental
challenges in teleost fish: identification of differentially expressed genes in the fish gill
and in regenerating fish skin. The data represents the largest gill and teleost skin
transcriptome to date and has been made publicly available.
-
A chemical survey for xenoestrogenic endocrine disrupting compounds (EDCs) in
Portugal water ways and development and validation of methods, endpoint analysis and
biomarkers for monitoring disease and the impact of toxicants and environmental
change in marine fish and bivalves.
-
Establishment of a range of new technologies and capacities by groups in the research
line to enhance R&D capabilities. Including establishment of a new method for
subtractive hybridization and cDNA library production, development of a medium
throughput strategy for recombinant protein production from genes from marine
organisms and isolation of embryonic stem (ES) cells from fish.
-
Development and refinement of the program GROMACS for classical protein dynamics
to include the effect of quantum amide I vibrational excitations and also arising from
computer simulations the proposal of new kinetic mechanisms to explaining protein
folding.
-
Consolidation of links and collaborations with Agência Ciência Viva and Centro de
Ciência Viva do Algarve to collaborate in the communication of the science to schools
and the general public.
1. Collaborative publications in peer review journals (If more that one Research Groups of
1. Barata, EN, Hubert, F, Conceicao, LEC, Velez, Z, Rema, P, Hubbard, PC, Canario, AVM. 2009.
Prey odour enhances swimming activity and feed intake in the Senegalese sole. Aquaculture
293: 100-107.
39
Comparative Biochemistry and Physiology C - Toxicology & Pharmacology 149: 58-64.
species based on Dynamic Energy Budgets. Journal of Sea Research 62:75-82.
4. Kadar, E, Lobo-da-Cunha, A, Azevedo, C. 2009. Mantle-to-shell CaCO3 transfer during shell
repair at different hydrostatic pressures in the deep-sea vent mussel Bathymodiolus azoricus
(Bivalvia: Mytilidae). Marine Biology 156: 959-967.
5. Laize, V, Tiago, DM, Aureliano M, Cancela, ML. 2009. New insights into mineralogenic effects
of vanadate. Cellular and Molecular Life Sciences 66(24): 3831-3836.
derived plastids. BMC Evolutionary Biology 9: 253.
salt marsh: a metal clean area?. Environmental Monitoring and Assessment 159: 191-205.
8. Silva, P, Valente, LMP, Galante, MH, Andrade, CAP, Monteiro, RAF, Rocha, E. 2009. Dietary
protein content influences both growth and size distribution of anterior and posterior muscle
fibres in juveniles of Pagellus bogaraveo (Brunnich). Journal of Muscle Research and Cell
Motility 30: 29-39.
9. Silva, P, Valente, LMP, Olmedo, M, Galante, MH, Monteiro, RAF, Rocha, E. 2009. Hyperplastic
and hypertrophic growth of lateral muscle in blackspot seabream Pagellus bogaraveo from
hatching to juvenile. Journal of Fish Biology 74: 37-53.
exposure to low levels of ethinylestradiol. Aquatic Toxicology 95: 330-338.
language)
2. Ribeiro, L, Hubbert, F, Rojas-García, C, Rønnestad, I, Engrola, S, Dinis, MT, Power, D. 2009. Cofeeding in Senegalese sole at mouth opening: Consequences on digestive physiology. In: Hendry,
CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds.). Larvi’09 - Fish & Shellfish Larviculture
Symposium. European Aquaculture Society, Special Publication nº38: 362-365
40
MASTER THESES
From the Master theses completed in 2009, 6 were in collaboration with RG within the RL (4)
and with national (2) institutions.
Araújo, A. 2009. Presença de Legionella sp. em redes de distribuição hospitalar e sua
epidemiologia. Master thesis in Biology and Management of Water, Faculty of Sciences,
University of Porto. Supervisor: Maria do Céu Lamas (Escola Superior de Tecnologia da Saúde do
Porto), Co-supervisor: JA Sousa (CIMAR-Pathology).
Gomes, JMBC. 2009. Efeito da inclusão de alimento vivo no desenvolvimento do estímulo
predatório e na performance do crescimento de juvenis de truta (Salmo trutta). Master thesis,
University of Trás-os-Montes and Alto Douro (UTAD) and ICBAS, University of Porto. Supervisor:
P Rema (CIMAR-LANUCE), Co-supervisor: JF Gonçalves (CIMAR-Ecophysiology).
Gomes, P. 2009. A importância da assinatura química elementar dos otólitos de Spondyliosoma
cantharus (n.v. choupa) na discriminação dos stocks pesqueiros e no estudo da estrutura
populacional. Master thesis in Marine Biology, Faculty of Marine and Environmental Science,
University of the Algarve. Supervisor: A Correia (CIMAR-Ecophysiology), Co-supervisor: K Erzini
(CIMAR-FBC)
Leitão, AF. 2009. Caracterização dos genes Gase, GSase e GDH em Misgurnus anguillicaudatus.
Master thesis in Marine Biology, University of Aveiro. Supervisor: JM Wilson (CIMAREcophysiology), Co-supervisor: VM dos Santos Quintino (University of Aveiro).
Pipa, T. 2009. Discriminação do(s) stock(s) de Diplodus vulgaris (n.v. safia) na costa SW
portuguesa com recurso a assinaturas químicas elementares dos otólitos. Master thesis in
Marine Biology, Faculty of Marine and Environmental Science, University of the Algarve.
Supervisor: A Correia (CIMAR-Ecophysiology), Co-supervisor: K Erzini (CIMAR-FBC).
Silva, M. 2009. Contribuição do estudo da microestrutura e microquímica de otólitos de
singnatídeos para a Ecologia das espécies, Syngnathus abaster e Nerophis lumbriciformes.
Master thesis in Applied Ecology, Faculty of Science, University of Porto. Supervisor: A Correia
(CIMAR-Ecophysiology), Co-supervisor: N Monteiro (CIMAR-Biodiversity of Aquatic Ecosystems).
PhD THESES
From the PhD theses completed in 2009, 3 were in collaboration with RG within the RL (4) and
with national (1) and international (1) institutions.
Kolmakov, N. 2009. Pheromone olfactory receptors in fish: isolation and functional
characterization. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: AVM
Canario (CIMAR-CME), Co-supervisor: J Coimbra (CIMAR- Ecophysiology).
University of Porto. Supervisor: AVM Canario (CIMAR-CME), Co-supervisor: J Coimbra (CIMAREcophysiology).
41
Ramos, A. 2009. Molecular basis of isoprenoid biosynthesis in Dunaliella salina. PhD thesis,
University of Algarve. Supervisor: J Varela (CIMAR-MarBiotech), Co-Supervisor: Bertram Brenig
(Göttingen, Germany).
thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Rui Oliveira (ISPA), Cosupervisors: AVM Canario (CIMAR-CME) and J Coimbra (CIMAR-Ecophysiology).
7d. Future research
The broad spectrum of scientific areas covered by the diverse groups present in RL3 makes
opportunities for direct collaboration difficult. Moreover, the physical separation of the two
research poles one in Porto and one in Faro means there are few opportunities to meet
informally and exchange information and find areas of common interest. The absence of the
traditional scientific meeting in the last year involving all members of CIMAR represented a loss
of an important opportunity for all research groups to mix and identify common interests. A key
objective of RL3 in the future will be to stimulate more interaction between groups as this will
potentiate capacities and raise the profile of research and the centre.
conferences, patents/prototypes, industry and other Government/Organization contract
Organization of conferences/workshops
-
International Workshop on Interdisciplinary Approaches in Fish Skeletal Biology, 27-29
April, Tavira, Portugal.
-
6th European Conference on Marine Natural Products, 19-23 July, Porto, Portugal.
http://www.cimar.org/6ECMNP/
-
2009, Porto, Portugal. http://www.ciimar.up.pt/7aiec/index.html
-
Conferences/workshops programmed for 2010
-
42
4th SPB Clinical Biochemistry Workshop, Interaction between biochemistry and clinical
practice, 29 January 2010, Faro, Portugal.
Patent
-
Feed additives and their use for aquaculture and aquiculture. Provisional patent 104732
under approval by the Portuguese patent office.
Networking actions
The presence of RL3 members in working groups, platforms and networks represents an
important opportunity for increased visibility of RL3-CIIMAR and also for brokerage actions to
involve other groups of CIMAR in initiatives.
-
ASSEMBLE - Association of European Marine Biological Laboratories (EU FP7 Research
Infrastructure, www.assemblemarine.org); AVM Canario (CME) has played a
fundamental role in stimulating collaboration between groups of RL in the area of
networking actions. The awarding of ASSEMBLE project has provided opportunities for
groups in CIMAR to join a network of Marine Biology platforms in Europe.
-
European Technology Platform (EATIP Thematic Area 3, managing the biological
lifecycle); DM Power (CME) is an invited member of EATIP and this will serve to give
CIMAR access to this European platform.
-
Marine Board Working Group in Marine Biotechnology; the membership of A Kijjoa
(CBAMNP) to this working group will generate opportunities in this area for other
CIMAR groups.
Several members (Ecophysiology, LECEMA, Pathology) participated in the meetings promoted by
the North Regional Coordination and Development Commission (CCDR-N), contributing to the
elaboration of regional priorities for the sustainable development of marine research in the
North of Portugal and Galiza region that resulted in a final report “Regional Agenda for the Sea”,
published in February 2009. This action will play an essential role in defining research priorities
in the area of Marine research which will benefit all CIMAR members.
2. Future plans
All the research groups involved in RL3 will aim to consolidate their impact on their existing
areas of expertise and capacities by seeking to publish in good impact journals, apply for funding
and when appropriate establish complimentary collaborations. The research area now contains
a number of well consolidated groups, and it is our intention to seek an even greater synergy
among groups in the line (and in other lines), with the aim of stimulating multidisciplinary
research. In the next year, special attention will be given to:
-
Identification of a “vision for the future“ of RL3 in order to define research priorities and
infrastructures essential to develop emerging research areas and stimulate the
establishment of relevant “technology platforms”.
-
Establishment of improved communication networks and build an overall team spirit in
RL3 to enhance resource sharing and strengthen actions in the domain of collaborative
training for MSc and PhD; in this context an inventory of capacities should be
established and publicized to stimulate integration in training actions.
43
-
Promotion of links with industry; in line with aims identified in 2008, steps have been
taken through the joint collaboration of CCMAR and CIIMAR in the successfully
submission during 2009 of an INTERREG IV B Atlantic Area project (ShareBiotech Sharing life science infrastructures and skills to benefit the Atlantic area biotechnology)
to stimulate technology transfer; the project starts in the beginning of 2010 and will
permit more effective action in this area in the future.
-
Further consolidation of outreach activities successfully initiated in 2008 and
strengthening of collaboration with Agência Ciência Viva (CCVIVA) and Centro de Ciência
Viva do Algarve (CCVALG), and support and engagement in the activities of the CMIA de
Vila do Conde, CMIA de Matosinhos, and Aquamuseu do Rio Minho, by further
encouraging participation by members of RL3.
Moreover, specific scientific areas were developed in frontier science which crosses more
than one research area as was foreseen in the 2008 RL3 report. Steps need to be taken to
now structure the research line and identify common and specific research goals and
priorities and develop a cohesive plan to stimulate excellence in basic and applied research.
44
RL4 - Aquaculture
1. Designation
Aquaculture
Maria Teresa Dinis
Marine Sciences
The objective of RL is to promote the generation of high quality scientific knowledge through
basic and applied research, to tackle some of the sustainability challenges facing the
aquaculture industry.
Strategic research areas were defined and clearly consolidated in the reporting period:
-
Biologic assessment of the cultivation potential of new aquaculture species, for the
establishment of sustainable culture practices to facilitate the industrialization process
in areas of broodstock management, larval rearing, nutrition growth, flesh quality of
new species for Mediterranean aquaculture.
-
Fish Nutrition, which fostered a better understanding of the nutritional modulation of
intermediary metabolism, oxidative stress damage, nutritional requirements, utilization
of alternative feedstuffs to fish meal and oil and optimal feed formulations for enhanced
performance, improved quality of fish, lower environmental impact.
-
Fish Immunology & Health aiming a better understanding of host/pathogens interaction
mechanisms and the establishment of immunostimulation strategies to enhance stress
resistance and welfare of farmed fish
The collaboration between the groups of RL4 was reinforced (evident in the publication list, PhD
and MSc theses co-supervised, joint research projects). The major achievement of the RL is its
contribution through basic knowledge and practical collaboration with the industry to the
establishment of intensive and semi-intensive farming systems for new species in the
Mediterranean area and enhancement of performance of well established species through
nutrition and sustainable practices.
45
Main scientific achievements:
-
Optimization of dietary formulations and feeding practices for new aquaculture species
such as blackspot seabream, Senegalese sole, white seabream, two banded seabream
and increase of nutritional knowledge for well established marine species (European sea
bass, gilthead sea bream and turbot).
-
Development of immunostimulation strategies to enhance stress resistance and
immune response in Senegalese sole.
-
Development of a practical diet with low levels of marine-derived proteins for
Senegalese sole, using the data generated on the nutritional requirements of juveniles.
It was also confirmed that early weaning may pose an additional (nutritional) stress
during metamorphosis.
-
Achievement a successful pilot-scale testing of an eco-friendly practical diet (low
fishmeal levels, reduced soluble phosphorus losses) for gilthead sea bream
-
Optimization of nutrient requirements, dietary formulations and feeding practices for
blackspot seabream and reinforcement of research on the nutritional requirements of
Diplodus sp. aiming a comparative evaluation among species and establishing the
potential for aquaculture of these promising omnivorous fish species. Due to the
importance for the industry, information regarding nutritional requirements, food
utilization and behavior of ornamental fish were considered.
-
The bottleneck of Octopus culture is the rearing of its paralarval and microalgal species
were used to improve the biochemical composition of juvenile Artemia as prey for
paralarvae.
-
Pursue the valorization of aquaculture products potential following 2 vectors: benefits
evaluation for human health of the incorporation of conjugated linoleic acid in the diets
and evaluating sensory, physical, microbiological characterization of freshness in
products
1. Collaborative publications in peer review journals (If more that one Research Groups of
293: 100-107.
46
2. Borges, P, Oliveira, B, Casal, S, Dias, J, Conceicão, L, Valente, LMP. 2009. Dietary lipid level
affects growth performance and nutrient utilization of Senegalese sole (Solea senegalensis)
juveniles. British Journal of Nutrition 102: 1007-1014.
3. Dias, J, Conceição, L, Ribeiro, AR, Borges, P, Valente, LMP, Dinis, MT. 2009. Practical diet with
low fish-derived protein is able to sustain growth performance in gilthead seabream (Sparus
aurata) during the grow-out phase. Aquaculture 293: 255-262.
4. Engrola, S, Figueira, L, Conceicao, LEC, Gavaia, PJ, Ribeiro, L, Dinis, MTD. 2009. Co-feeding in
Senegalese sole larvae with inert diet from mouth opening promotes growth at weaning.
Aquaculture 288: 264-272.
5. Gavaia, PJ, Domingues, S, Engrola, S, Drake, P, Sarasquete, C, Dinis, MT, Cancela, ML. 2009.
Comparing skeletal development of wild and hatchery-reared Senegalese sole (Solea
senegalensis, Kaup 1858): evaluation in larval and postlarval stages. Aquaculture Research 40:
1585-1593.
Ecotoxicology and Environmental Safety 72:473-478.
7. Ozório, ROA, Andrade, C, Conceição, LEC, Timóteo, VMFA, Valente, LMP. 2009. Effects of
feeding levels on growth response, body composition and energy expenditure in blackspot
seabream Pagellus bogaraveo juveniles. Journal of World Aquaculture Society 40: 95-103.
8. Ozorio, ROA, Valente, LMP, Correia, S, Pousao-Ferreira, P, Damasceno-Oliveira, A, Escorcio, C,
Oliva-Teles, A. 2009. Protein requirement for maintenance and maximum growth of two banded
seabream (Diplodus vulgaris) juveniles. Aquaculture Nutrition 15: 85-93.
75, 661-666.
10. Silva, JMG, Espe, M, Conceicão, LEC, Dias, J, Valente, LMP. 2009. Senegalese sole juveniles
(Solea senegalensis Kaup, 1858) grow equally well on diets devoid of fish meal provided the
dietary amino acids are balanced. Aquaculture 296: 309-317.
Motility 30: 29-39.
hatching to juvenile. Journal of Fish Biology 74: 37-53.
47
2. Collaborative other publications (Include only if more than one group is involved and
only include here Books, chapters or full papers published in conference proceedings.
Give title and full citation in original language)
1. Aragão C, Weber, RA, Costas, B, Dinis, MT, Conceição, LEC. 2009. Effects of ammonia
exposure in Senegalese sole (Solea senegalensis) larvae. In: Hendry, CI, Van Stappen, G, Wille,
M, Sorgeloos, P. (Eds.). Larvi’09 - Fish & Shellfish Larviculture Symposium. European Aquaculture
Society, Special Publication nº38: 14-17.
CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). Larvi’09 - Fish & Shellfish Larviculture
Symposium. European Aquaculture Society, Special Publication nº38: 362-365
MASTER THESES
From the Master theses completed in 2009, 5 were in collaboration with RG within and outside
the RL (3) and with national (1) and international (1) institutions.
Borges, PAC. 2009. Optimização do nível lipídico em dietas para juvenis de linguado. Master
thesis, Faculty of Pharmacy, University of Porto. Supervisor: Beatriz Oliveira (Faculty of
Pharmacy, University of Porto), Co-supervisor: L Valente (CIMAR-LANUCE).
Cruzeiro, CR. 2009. Efficacy of a phospholipase A2 (aPLA2) antibody on growth performance of
juvenile fish fed a practical diet. Master thesis, University of Trás-os-Montes and Alto Douro
(UTAD). Supervisor: P Rema (CIMAR-LANUCE), Co-supervisor: J Dias (CIMAR-Aquagroup).
Gomes, JM. 2009. Efeito da inclusão de alimento vivo no desenvolvimento do estímulo
P Rema (CIMAR-LANUCE), Co-supervisor: JF Gonçalves (CIMAR-Ecophysiology).
Pedrosa, KP. 2009. Efeito da inclusão na dieta de óleo e farinha de canabinoides na ingestão
alimentar de juvenis de rodovalho Scophtalmus maximus. University of Trás-os-Montes and Alto
Douro (UTAD). Supervisor: P Rema (CIMAR-LANUCE), Co-supervisor: J Dias (CIMAR-Aquagroup).
PhD THESES
From the PhD theses completed in 2009, 5 were in collaboration with RG within the RL (2) and
with international (4) institutions.
Laporte, J. 2009. Nutritional evaluation of animal by-products for the partial replacement of fish
meal in diets for gilthead sea bream Sparus aurata. PhD thesis, School of Biological Sciences,
Faculty of Sciences, University of Plymouth, UK. Supervisor: S Davies (University of Plymouth),
Co-supervisor: A Gouveia (CIMAR-Fish Nutrition).
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Seixas, P. 2009. Composición bioquímica y crecimento de paralarvas de pulpo (Octopus vulgaris
Cuvier, 1797), alimentadas com juveniles de Artemia enriquecidos com microalgas y otros
suplementos nutricionales. PhD thesis in Aquatic Sciences, ICBAS, University of Porto.
Supervisor: Manuel Rey Mèndez (Universidade de Santiago de Compostela), Co-supervisor: Ana
Otero (Univ Santiago de Compostela), L Valente (CIMAR-LANUCE).
Silva, ACF. 2009. Regulação nutricional da deposição lipídica em goráz (Pagellus bogaraveo). PhD
in Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Valente (CIMAR-LANUCE), Cosupervisor: Geneviéve Corraze (Station de Recherches en Hydrobiologie, INRA, France).
Silva, JMG. 2009. Effectiveness of alternative protein sources in meeting the true amino acid
requirements of Senegalese sole. PhD in Aquatic Sciences, ICBAS, University of Porto.
Supervisor: L Valente (CIMAR-LANUCE), Co-supervisors: Marite Espe (NIFES, Norway), Luís
Conceição (CIMAR- Aquagroup).
Silva, PCV. 2009. Differentiation, development and growth of the blackspot seabream (Pagellus
bogaraveo) muscle. PhD thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisor: E
Rocha (CIMAR-LECEMA), Co-supervisor: L Valente (CIMAR-LANUCE).
7d. Future research
Members of the RL4 were involved in industry and other Government/Organization contract
Conferences/workshops programmed for 2010:
-
Aquaculture Europe 2010, 5-8 October 2010, Porto, Portugal
Industry and other Government/Organization contract research:
-
OPTIDIETAS (IDEIA 70/00073), in consortium with a feed company SORGAL, S.A. for
supporting the development of applied research in companies. This project is focused
on the optimization of dietary formulations and feeding practices for Senegalese sole
and blackspot seabream, based on the specific nutritional requirements and feeding
behaviour of each species.
Networking actions:
-
European Aquaculture Technology and Innovation Platform. www.eatip.eu
-
Water Supply and Sanitation Technology Platform. www.wsstp.eu
-
Welfare of fish in European aquaculture - Cost Action n. 86.
49
-
Critical success factors for fish larval production in European Aquaculture: a
multidisciplinary network - Cost Action n. FA0801
Several members (LANUCE, Fish Nutrition) participated in the meetings promoted by the North
Regional Coordination and Development Commission (CCDR-N), contributing to the elaboration
of regional priorities for the sustainable development of marine research in the North of
Portugal and Galiza region that resulted in a final report “Regional Agenda for the Sea”,
published in February 2009. This action will play an essential role in defining research priorities
in the area of Marine research which will benefit all CIMAR members.
The Research groups within this RL are working on the establishment at CIMAR of a Fish
Nutrition area under a common umbrella, the designated CIMAR – Fish Nutrition Unit. In general
terms, its mission is to promote and consolidate the rationale development of the CIMAR
expertise and activities in the area of Fish Nutrition, and become an indispensable contributor in
the elaboration of regional priorities for the sustainable development of marine research.
2. Future plans
The research objectives of the Aquaculture line for the next years are clearly identified and in
line with some of the evolving sustainability challenges facing the aquaculture industry. The
team assembles a range of competencies that allow a multidisciplinary approach of several
research themes, which have been integrated in the following key areas:
50
-
Nutrition for Enhanced Performance
-
Sustainable Practices
-
Enhancement of health status
-
Flesh quality
-
Security of fisheries and aquaculture products
8. RESEARCH GROUPS
1. Research group
Principal Investigator
Name of the Research Groups
Maria Teresa Dinis
Aquaculture Research Group (Aquagroup)
Isabel Maria Trigueiros Sousa Pinto Machado
Biodiversity of Aquatic Ecosystems
Maria Ester Tavares Alvares Serrao
Biogeographical Ecology and Evolution (BEE)
Maria Leonor Nunes Ribeiro Cruzeiro
Manuel Aureliano Pereira Martins Alves
Eduardo Jorge Sousa Rocha
Biophysics
BioVanadium Research Group, now
consolidated as Cellular and Inorganic
Biochemistry (CIB)
Cellular and Molecular Studies, now
consolidated as Cellular, Molecular and
Analytical Studies (LECEMA)
Maria Teresa Sá Dias de Vasconcelos
Chemical Speciation and Bioavailability (CS&B)
Anake Kijjoa
Chemistry and Biological Activity of Marine
Natural Products
Adelino Vicente Mendonca Canario
Comparative Molecular Endocrinology (CME)
Luis Manuel Zambujal Chicharo
Ecology and Restoration of Estuarine and
Coastal Habitats (ECOREACH)
Ecophysiology
Ecotoxicology (ECOTOX)
Vitor Manuel Oliveira Vasconcelos
Ecotoxicology, Genomics and Evolution (LEGE)
Maria Clara Semedo da Silva Costa
Environmental Technologies
Maria Armanda Reis Henriques
Environmental Toxicology
51
Maria Leonor Quintais Cancela Fonseca
Evolution, Development and Gene Expression
(EDGE)
Aires Manuel Pereira Oliva Teles
Fish Nutrition
José Pedro de Andrade e Silva Andrade
Fisheries Biology and Hydroecology Research
Group (FBHRG)
Karim Erzini
Fisheries, Biodiversity and Conservation
Geology and Paleoceanography
Adriano Agostinho Donas Bôto Bordalo e Sá
Hydrobiology
João Carlos Serafim Varela
Biotechnology and Molecular Biology of
Microalgae (BMBM), now consolidated as
Marine Biotechnologies (MarBiotech)
Rui Orlando Pimenta Santos
Marine Plant Ecology (ALGAE)
Rodrigo da Silva Costa
Microbial Ecology and Evolution
Luísa Maria Pinheiro Valente
Nutrition, Growth and Quality of Fish (LANUCE)
Maria Luisa Machado Cerqueira Bastos
Oceanic and Coastal Dynamics
Jorge Guimaraes da Costa Eiras
Pathology
Cymon John Cox
Plant Systematics and Bioinformatics
Maria Lurdes Santos Cristiano
Synthesis and Organic Reactivity
52
Aquaculture Research Group (Aquagroup)
8a. Group description
1. Group name / denomination
Aquaculture research group (Aquagroup)
Maria Teresa Dinis
3. Location of group
CCMAR - Centro de Ciências do Mar, Faro
8b. Funding, sources, dates
In 2009, the Aquagroup was involved in 7 research projects, in all of them with a coordination
role. The majority of projects (5) were funded through FCT, while 2 were undertaken by
European funding (FP6). These projects encompass a global funding (full duration of projects) of
1.08 Million EUR (including overheads).
312.088 EUR, EU-FP6, SEACASE, Jan 07-Jan 10.
108.002 EUR, FCT, FATTYBONE, Aug 08-Jul 11.
155.128 EUR, FCT, TEXBREAM, Aug 08-Jul 11.
171.002 EUR, FCT, HYDRAA, Jul 08-Jun 11.
189.785 EUR, FCT, CRYOSPERM, Apr08-Mar 11.
95.122 EUR, FCT, EFARFish, Oct 08-Sep 11.
50.000 EUR, INTERREG, ECOAQUA, Apr 09-Sep 11.
8c. Objectives and achievements
1. Objectives
The mission of the Aquaculture Research Group is to generate scientific knowledge, through
basic and applied research, to tackle some of the evolving sustainability challenges facing the
aquaculture industry. Our skills rely heavily on our expertise on broodstock management, larval
rearing and nutrition of new species for Mediterranean aquaculture. The team assembles a
53
range of competencies that allow a multidisciplinary approach of several research themes,
which have been integrated in two key areas:
I. Nutrition for enhanced performance
-
The nutritional and metabolic utilization of nutrients in marine fish larvae and juveniles.
Projects on this topic (HYDRAA, FATTYBONE, EFARFish), comprise studies on the role of
amino acids, peptide fractions and essential fatty acids in areas such as digestive
efficiency, intermediary metabolism, immune competence and skeletal deformities in
marine fish. Besides classical zootechnical and biochemical assessment criteria, most
projects integrate also functional genomics, proteome expression, tracer studies and
metabolic modeling approaches.
-
Broodstock Management of new species (sole, dusky grouper) with a strong emphasis
on nutrition and welfare (ECOAQUA) and the improvement of semen cryopreservation
success in various farmed species (seabass, seabream and turbot) (CRYOSPERM)
through the addition of supplements such as vitamins and aminoacids to reduced
oxidative stress damage.
-
A new research topic on the Quality and Safety of Fish has been implemented. By
associating proteome analysis and traditional criteria, we intend to generate new
knowledge on the role of nutritional factors and farming practices as modulators of
flesh softening and aroma in farmed fish (TEXBREAM).
II. Sustainable practices
-
Research on the Production Systems topic has tackled aspects related to the
implementation of new aquaculture species through the estimation of nutritional
requirements for sole, blackspot seabream and white bream and the development of
effective tools for the assessment of competitiveness and sustainability of extensive and
semi-intensive aquaculture production in Southern Europe (SEACASE).
-
Research activities on Fish Welfare have also been reinforced. Issues addressed are the
dietary modulation of the immune status in fish larvae (PhD project) and the definition
of operational welfare indicators in broodstock fish (ECOAQUA).
Societal
-
A major achievement of the Aquagroup has been its ongoing contribution to the
optimization of Senegalese sole farming in intensive and semi-intensive systems.
Knowledge generated throughout several previous and ongoing projects contributes
decisively for today’s progressive establishment of large scale commercial farming of
sole in Portugal and Spain.
-
Successful granting of 2 new research projects with coordination by Aquagroup
members.
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-
Through its central role in the SEACASE project, the Aquagroup has reinforced its
dialogue with the Portuguese semi-intensive fish farming industry, creating a strong
momentum in its strategy “from lab to farm”, for instance with its activities in the
development of eco-friendly feeds for semi-intensive fish farming. The societal
relevance of this project is reflected in several radio and newspapers interviews, and
also a national TV interview.
Scientific
-
Successful pilot-scale testing of an eco-friendly practical diet (low fishmeal levels and
reduced soluble phosphorus losses) for gilthead seabream.
-
Data generated on the nutritional requirements of Senegalese sole juveniles (protein,
lipid, amino acids, and apparent digestibility of ingredients) allowed the development of
a practical diet with low levels of marine-derived proteins for this species.
-
Contrary to what is commonly seen in salmonid species, harvesting stress and slaughter
method have little detrimental effects on the flesh quality of gilthead seabream,
assessed through biochemical, instrumental and sensory criteria.
-
Despite no major detrimental effects on growth performance, dietary oil sources were
found to alter bone metabolism and deposition pattern significantly in gilthead
seabream juveniles.
-
Further insight was obtained on the digestive capacity of Senegalese sole for both
protein and lipids, and its relation with feeding regime, through the use of tracer
studies. In particular it was confirmed that early weaning may pose an additional
(nutritional) stress during metamorphosis.
-
Evidence for coping styles was identified in Senegalese sole juveniles, through the study
of individual differences in cortisol levels and behaviour.
-
A successful cryopreservation protocol of sperm from sex-reversed dusky grouper.
-
Characterization of seabass sperm quality during the reproductive season, in terms of
freezability.
-
Reduction of oxidative stress damage in cryopreseved sperm from seabass and
seabream by antioxidant supplementation of the freezing media.
Career building for young researchers
Three students completed their MSc degrees in our group in 2009:
-
Castanheira, MF. 2009. Metabolic basis of growth variation in juvenile sole (Solea
senegalensis, Kaup 1858). Master thesis, Faculty of Sciences and Technology, University
of Algarve. Supervisors: L Conceição, S Engrola.
55
-
Gonçalves, R. 2009. Determination of dietary docosahexaenoic acid (DHA) requirements
for Senegalese sole (Solea senegalensis) post-larvae. Master thesis, Faculty of Sciences
and Technology, University of Algarve. Supervisors: L Conceição, DA Martins.
-
Rocha, F. 2009. Requisitos de ácido araquidónico em microdietas para larvas de
Dourada (Sparus aurata, L., 1758). Master thesis, Faculty of Sciences and Technology,
University of Algarve. Supervisors: L Conceição, DA Martins.
8d. Productivity
1. Publications in peer review journals
1. Barata, EN, Hubert, F, Conceicão, LEC, Velez, Z, Rema, P, Hubbard, PC, Canario, AVM. 2009.
293: 100-107. IF=1.925.
2. Beirão, J, Soares, F, Herraez, MP, Dinis, MT, Cabrita, E. 2009. Sperm quality evaluation in Solea
senegalensis during the reproductive season at cellular level. Theriogenology 72: 1251-1261.
IF=2.073.
3. Borges, P, Oliveira, B, Casal, S, Dias, J, Conceicão, L, Valente, LMP. 2009. Dietary lipid level
affects growth performance and nutrient utilisation of Senegalese sole (Solea senegalensis)
juveniles. British Journal of Nutrition 102: 1007-1014. IF=3.446.
4. Cabrita, E, Engrola, S, Conceicão, LEC, Pousão-Ferreira, P, Dinis, MT. 2009. Successful
cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus.
Aquaculture 287: 152-157. IF=1.925.
5. Calado, R, Vitorino, A, Reis, A, Lopes da Silva, T, Dinis, MT. 2009. Effect of different diets on
larval production, quality and fatty acid profile of the marine ornamental shrimp Lysmata
amboinensis (de Man, 1888), using wild larvae as a standard. Aquaculture Nutrition 15: 484-491.
IF=1.482.
6. Carvalho, S, Falcão, M, Cúrdia, J, Moura, A, Serpa, D, Gaspar, MB, Dinis, MT, Pousão-Ferreira,
P, Cancela da Fonseca, L. 2009. Benthic dynamics within a land-based semi-intensive
aquaculture fish farm: the importance of settlement ponds. Aquaculture International 17: 571587. IF=0.753.
7. Conceicão, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2009. Avanços
recentes em nutrição de larvas de peixes. Revista Brasileira de Zootecnia 38: 26-35. IF=0.557.
8. Cunha, ME, Quental, H, Barradas, A, Pousão-Ferreira, P, Cabrita, E, Engrola, S. 2009. Rearing
larvae of dusky grouper, Epinephelus marginatus (Lowe, 1834), (Pisces: Serranidae) in a semiextensive mesocosm. Scientia Marina 73(S1): 201-212. IF=1.174.
56
9. Dias, J, Conceicão, LEC, Ribeiro, AR, Borges, P, Valente, LMP, Dinis, MT. 2009. Practical diet
with low fish-derived protein is able to sustain growth performance in gilthead seabream
(Sparus aurata) during the grow-out phase. Aquaculture 293: 255-262. IF=1.925.
10. Engrola, S, Figueira, L, Conceição, LEC, Gavaia, PJ, Ribeiro, L, Dinis, MT. 2009. Co-feeding in
Aquaculture 288: 264-272. IF=1.925.
11. Engrola, S, Mai, M, Dinis, MT, Conceição, LEC. 2009. Co-feeding of inert diet from mouth
opening does not impair protein utilization by Senegalese sole larvae. Aquaculture 287: 185-190.
IF=1.925.
senegalensis, Kaup 1858): evaluation in larval and postlarval stages. Aquaculture Research 40:
1585-1593. IF=1.099.
13. Mai, MG, Engrola, S, Morais, S, Portella, MC, Verani, JR, Dinis, MT, Conceicão, LE. 2009. Cofeeding of live feed and inert diet from first-feeding affects Artemia lipid digestibility and
retention in Senegalese sole (Solea senegalensis) larvae. Aquaculture 296: 284-291. IF=1.925.
14. Makridis, P, Moreira, C, Costa, RA, Rodrigues, P, Dinis, MT. 2009. Use of microalgae
bioencapsulated in Artemia during the weaning of Senegalese sole (Solea senegalensis Kaup).
Aquaculture 292: 153-157. IF=1.925.
15. Morais, S, Conceicão, LEC. 2009. A new method for the study of essential fatty acid
requirements in fish larvae. British Journal of Nutrition 101: 1564-1568. IF=3.446.
16. Oliveira, C, Dinis, MT, Soares, F, Cabrita, E, Pousão-Ferreira, P, Sanchez-Vazquez, FJ. 2009.
Lunar and daily spawning rhythms of Senegal sole Solea senegalensis. Journal of Fish Biology 75:
61-74. IF=1.226.
17. Ozório, ROA, Andrade, C, Timóteo, VMFA, Conceicão, LEC, Valente, LMP. 2009. Effects of
feeding levels on growth response, body composition and energy expenditure in blackspot
seabream Pagellus bogaraveo juveniles. Journal of World Aquaculture Society 40: 95-103.
IF=0.780.
18. Perez-Cerezales, S, Martinez-Paramo, S, Cabrita, E, Martinez-Pastor, F, Herraez, MP. 2009.
Evaluation of oxidative DNA damage promoted by storage in sperm from sex-reversed rainbow
trout. Theriogenology 71: 605-613. IF=2.073.
19. Pinto, W, Figueira, L, Dinis, MT, Aragão, C. 2009. How does fish metamorphosis affect
aromatic amino acid metabolism? Amino Acids 36: 177-183. IF=3.877.
20. Saavedra, M, Barr, Y, Pousão-Ferreira, P, Helland, S, Yúfera, M, Dinis, MT, Conceicão, LEC.
2009. Supplementation of tryptophan and lysine in Diplodus sargus larval diet: effects on growth
and skeletal deformities. Aquaculture Research 40: 1191-1201. IF=1.099.
57
21. Saavedra, M, Pousão-Ferreira, P, Yúfera, M, Dinis, MT, Conceição, LEC. 2009. A balanced
amino acid diet improves Diplodus sargus larval quality and reduces nitrogen excretion.
Aquaculture Nutrition 15: 517-524. IF=1.482.
22. Silva, JMG, Espe, M, Conceicão, LEC, Dias, J, Valente, LMP. 2009. Senegalese sole juveniles
dietary amino acids are balanced. Aquaculture 296: 309-317. IF=1.925.
2. Other publications international (Books, chapters or full papers published in conference
proceedings)
1. Rodrigues, P, Silva, T, Jessen, F, Dias, J. 2009. On the reproducibility of a fractionation
procedure for fish muscle proteomics. Molecular & Cellular Proteomics 8: S-19.
2. Aragão C, Weber, RA, Costas, B, Dinis, MT, Conceição, LEC. 2009. Effects of ammonia
exposure in Senegalese sole (Solea senegalensis) larvae. In: Hendry, CI, Van Stappen, G, Wille,
M, Sorgeloos, P. (Eds). Larvi’09 - Fish & Shellfish Larviculture Symposium. European Aquaculture
3. Conceição, LEC, Rønnestad, I. 2009. A dynamic model for dietary amino acids utilisation in fish
larvae. In: Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). Proceedings of the Larvi’09 Fish & Shellfish Larviculture Symposium. European Aquaculture Society, Special Publication
nº38.
4. Cunha, ME, Quental Ferreira, H, Barradas, A, Pousão-Ferreira, P, Cabrita, E, Engrola, S. 2009.
Growth and development of Dusky grouper Epinephelus marginatus larvae in mesocosm of
semi-intensive technology. In: Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds).
Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium. European Aquaculture
5. Engrola, S, Dinis, MT, Conceição, LEC. 2009. Senegalese sole is able to adapt protein
metabolism when co-fed with Artemia replacement. In: Hendry, CI, Van Stappen, G, Wille, M,
Sorgeloos, P. (Eds). Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium.
European Aquaculture Society, Special Publication nº38: 84-87.
6. Morais, S, Alves Martins, D, Castanheira, F, Mendes, A, Coutinho, J, Bandarra, NM, PousãoFerreira, P, Conceição, LEC. 2009. A new method for the study of essential fatty acid
requirements in fish larvae. In: Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds.).
Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium. European Aquaculture
7. Pinto, W, Figueira, L, Rodrigues, V, Dinis, MT, Aragão, C. 2009. The importance of aromatic
amino acids during fish metamorphosis. In: Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P.
(Eds). Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium. European
Aquaculture Society, Special Publication nº38: 329-332.
58
Symposium. European Aquaculture Society, Special Publication nº38: 362-365.
3. Other publications national (Books, chapters or full papers published in conference
proceedings)
4. Master and PhD theses completed
MASTER THESES
Castanheira, MF. 2009. Metabolic basis of growth variation in juvenile sole (Solea senegalensis,
Kaup 1858) 2009. Master thesis, Faculty of Sciences and Technology, University of Algarve.
Supervisors: L Conceição, S Engrola.
(UTAD). Supervisor: P Rema, Co-supervisor: J Dias.
Gonçalves, R. 2009. Determination of dietary docosahexaenoic acid (DHA) requirements for
Senegalese sole (Solea senegalensis) post-larvae. Master thesis, Faculty of Sciences and
Technology, University of Algarve. Supervisors: L Conceição, DA Martins.
alimentar de juvenis de rodovalho Scophtalmus maximus. Master thesis, University of Trás-osMontes and Alto Douro (UTAD). Supervisor: P Rema, co-supervisor: J Dias.
Rocha, F. 2009. Requisitos de ácido araquidónico em microdietas para larvas de Dourada (Sparus
aurata L., 1758). Master thesis, Faculty of Sciences and Technology, University of Algarve.
Supervisors: L Conceição, DA Martins.
5. Patents/propotypes
6. Organization of conferences
7. Industry contract research
Over the years, the scientific expertise and reliable experimental facilities associated to the
Aquaculture Research Group have gained recognition by the aquaculture industry. In 2009, the
Aquagroup conducted contract-research activities with two newly created technology-based
SME’s:
-
Vale I&DT INOVSEA - Production of polychaetes as feed for broodstock fish in
aquaculture. Company: INOVSEA Lda.
59
-
Vale I&DT MARSENSING - Potential application of underwater acoustic stimuli in marine
aquaculture. Company: MarSensing Lda.
8. Internationalization (Collaborative publication, Research, Graduate Training Networks
or other forms of participation of the Research Group at the international level)
The Aquaculture Research Group has a long standing tradition of associating itself to
international cooperation windows. Six PhD theses currently (2009) underway within the
Aquagroup benefit from structural collaborations with other European research institutions,
namely NIFES (Norway), BIO/UiB (Norway), NTU (former DIFRES, Denmark), and Bodø University
College (Norway). This aspect is also reflected by the fact that 11 out of the 23 (48%)
publications of the Aquagroup in 2009 were written in collaboration with colleagues from
institutions outside Portugal.
The Aquagroup members have also been active participants in the following networking
structures at European level:
-
COST Action 867 - Welfare of fish in European aquaculture. L Conceição is one of the
Portuguese representatives in the Management Committee.
-
European Aquaculture Technology and Innovation Platform (EATiP). J Dias is a member
of the working groups “Sustainable raw materials for fish feed production” and “Fish
health and welfare” devoted to the establishment of the Strategic Research Agenda. L
Conceição is the Chair of the workgroup 2 “Hatchery/Nursery production of fry and
spat”, of the Thematic Area 3 “Managing the Biological Lifecycle”.
-
COST Action FA0801 “LARVANET - Critical success factors for fish larval production in
European Aquaculture: a multidisciplinary network”. L Conceição is the Vice-Chair and
one of the Portuguese representatives in the Management Committee.
9. Government/organization contract research (Work carried out by the Research Group
that resulted in a publication or report. Of particular importance are those involved in
public policy advice)
8e. Future research
1. Objectives
The research objectives of the Aquaculture Research Group for the next years are clearly
identified and in line with some of the evolving sustainability challenges facing the aquaculture
industry. The team assembles a range of competencies that allow a multidisciplinary approach
of several research themes, which have been integrated in two key areas:
-
Nutrition for Enhanced Performance
-
Sustainable Practices
60
The nutritional and metabolic utilization of amino acids in fish larvae, by means of the
innovative approach of tracer studies is a highly successful research topic, which gave the
Aquagroup its most distinctive character at both National and International levels. This
established research line is to be further consolidated and broadened. Its development requires
integrative studies on the role of amino acids, peptide fractions, fatty acids and micronutrients
in areas such as digestive physiology, intermediary metabolism, immune competence and
skeletal deformities in marine fish. Our activities on this area will also focus towards the
assessment of nutritional factors (plant ingredient-rich diets) and farming practices as tools to
enhance the quality, health benefits and safety of farmed fish. Activities on the optimization of
broodstock management through improved nutrition and welfare, on innovative semen
cryopreservation and reproductive techniques, and the development of broodstock selection
programs for sole and dusky grouper are also foreseen. In all of these research topics, besides
classical zootechnical and biochemical assessment criteria, most projects integrate also
functional genomics, proteome expression, tracer studies and metabolic modeling approaches.
The access to functional genomic and proteome expression platforms is already under
implementation (in-house and through external collaborations). Two post-doc researchers and 4
PhD students are largely dedicated to this area.
The aquaculture industry in Portugal faces profound changes in the near future. Namely an
increase in production with the new off-shore cage farming systems, the entry into commercial
phase of several new species, and the necessity to maintain competitiveness on traditional
semi-intensive production systems. In this context, the Aquagroup identified the Production
Systems research area as a priority and an opportunity to strengthen its support to industrial
partners. Additionally, given its growing importance as a societal issue, the Fish Welfare
research line is to be reinforced within the Aquagroup. Research will focus on establishing basic
knowledge regarding the modulation of stress resistance in fish, aggressive behavior, evaluation
of bioactive compounds for enhanced health status and the definition of operational chronic
stress indicators. The implementation of this research area will be progressive within CCMAR
and in close collaboration with the Immunobiology Laboratory, from CIIMAR. One joint PhD
student is fully dedicated to this area.
The strategy of reinforcing the collaborations in the area of Fish Nutrition within CIMAR LA, to
gain competitiveness in both National and International research consortiums, is now currently
under implementation. The following teams have federated future research efforts on this area
under a common umbrella, the designated CIMAR LA – Fish Nutrition Unit.
-
Aquaculture Research Group at CCMAR, headed by Prof. Maria Teresa Dinis.
-
Fish Nutrition Group at CIIMAR, headed by Prof. Aires Oliva-Teles.
-
Laboratory of Fish Nutrition, Growth & Quality at CIIMAR, headed by Prof. Luísa Valente.
Within this structure, four joint research projects are currently under evaluation (FCT).
Additionally, several joint PhD projects are currently underway (2) or have been completed (2).
2. Funding, source, dates (Amount of current and pending funding)

Current funding (Approved, to start early in 2010)
61
110.000 EUR, FCT PTDC/CVT/102481/2008, ULTRAFISH, 2010-2013.
50.000 EUR, FCT PDTC/MAR/105152/2008, SPECIAL K, 2010-2013.
103.000 EUR, FCT PTDC/MAR/102348/2008, SEPIAMETA, 2010-2013.
95.000 EUR, EU FP7 Res. for benefit SMEs, PROSPAWN, 2010-2012.

Pending funding (under evaluation, 2009 call, approximately 1.52 Million EUR for the
period 2010-2013)
149.000 EUR, TAUSOLE.
95.000 EUR, DRUM.
98.000 EUR, SOSTRESS.
75.425 EUR, DUSK.
99.757 EUR, EPISOLE.
70.167 EUR, HIPPOBREED.
196.878 EUR, CRYOGERM.
159.442 EUR, LIPSTRESS.
199.807 EUR, RASTEROIDS.
174.928 EUR, COGNIFISH.
199.952 EUR, STRESSIND.
62
Isabel Maria Trigueiros Sousa Pinto Machado
CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto
-
57.000 EUR, FCT Pluriannual funding.
-
12.000 EUR PhD grants.
-
FP6, EC funding
107.000 EUR, Network of Excellence MARBEF, 2004-2009.
203.000 EUR, Era-Net BIODIVERSA, 2006-2010.
148.000 EUR, BIOSTRAT Developing the EU Biodiversity Research Strategy, 2006-2010.
-
Other
1.000.000 EUR, EEA ECClima Managing Effects of Global Climate on Estuarine Biodiversity and
Productivity, 2007-2011.
100.000 EUR, QREN, IBEROMARE Centro Multipolar de Valorização de Recursos e Resíduos
Marinhos, 2009-2011.
58.000 EUR, QREN, EEMA Avaliação do Estado Ecológicos das Massas de Água costeiras e de
transição e do potencial ecológico das massas de água fortemente modificadas, 2009-2011.
57.000 EUR, Axa Foundation, Marine Aliens and Climate Change, 2009-2011.
82.000 EUR, INTERREG III B Gestión sostenible de los recursos marinos, 2009-2012.
306.000 EUR, Dir Reg Ciência Tecnol (DRCT) - Unidade piloto de produção de óleo vegetal a
partir de microalgas, 2008-2010.
63
94.000 EUR, Sec Reg Amb Mar (SRAM) - Estudo da viabilidade de produção de lapa Patella e
Haliotis em aquacultura intensiva, 2007-2010.
286.000 EUR, SRAM - Caracterização das massas de água costeira S Maria, S Miguel, S Jorge e
Terceira, 2008-2009.
4000 EUR, AI LusoEspanhola, 2008-2009.
40.000 EUR, Parque Biológico de Gaia, 2008-2010.
4000 EUR, Petrobrás e UFRN, 2008-2011.
1. Objectives
The general objectives of the group are:
-
To increase knowledge and understanding of the different levels of organization of
marine biodiversity (from genes to organisms, populations, communities and
ecosystems), its importance for the functioning of the living world and the
consequences of anthropogenic pressures, particularly under current and predicted
scenarios of global environmental changes.
-
To increase our knowledge on a sustainable use of marine organisms, in order to
produce or improve economic resources without endangering the functioning of marine
ecosystems and the services they provide.
-
To create an awareness of general public and young people in particular about the
importance of biodiversity and of its role on the functioning of ecosystems and their
services and the need to preserve it.
-
To strengthen reciprocal links between researchers and policy makers and managers,
especially those responsible for marine and coastal areas and resources, in order to
promote policy relevant research and to increase the contribution of scientific results to
political decisions and management.
Within this framework the specific objectives of our group are:
-
64
To determine the patterns of distribution and abundance of species on benthic
communities and their variation at different spatial and temporal scales, across their
geographic range of distribution in European waters, with special focus on communities
along the Portuguese coast. This knowledge will contribute directly to coastal and
resource management, particularly for the Northern region and the Azorean
Archipelago.
-
To assess the effects of different stressors on coastal biodiversity, including estuarine
systems, detected from the level of individual physiological responses to the functional
effects at the ecosystem level, and using different approaches: from ecophysiological
measurements to observational studies, demographic modelling and experimental
manipulations of assemblages.
-
To evaluate the structure and dynamics of biotic interactions and food webs in coastal
and estuarine systems, through the use of traditional and stable isotope signatures
methods.
-
To evaluate the use of macroalgae as water purifiers and as an aquaculture product
through the implementation of Integrated Multitrophic Aquaculture systems (IMTAs), in
collaboration with existing commercial fish farms.
-
To develop new products from marine organisms, and increase the value of species
used to improve water quality (IMTA systems) or discard organisms.
Besides these research objectives we also aim at:
-
Producing outreach programs and materials for the general public and school children
and improve public awareness and understanding of links and specificity of taxonomy,
ecology and evolution, and of the increasing need and urgency for applying a scientific
approach integrating these disciplines to coastal management and nature conservation.
-
Promoting research that is relevant to the conservation and sustainable use of marine
biodiversity, specially under a climate change context.
-
To improve the effectiveness of communication and cooperation between researchers
and policy makers in order to implement scientifically-based policies and action plans, at
regional, National and European level, including adaptation and mitigation strategies
under climate change and biological invasions scenarios. This goal is strengthened
through the development of programs for environmental characterization and long
term data acquisition, in cooperation with and under funding by regional institutions.
In order to strengthen the group, in 2009 we welcomed and integrated four new researchers,
hired through the Programa Ciência 2008, and applied for research funding for new researchers
and for FP7. We set-up two new facilities, buying new equipment and increasing significantly the
space available for the lab. We also completely renovated our prototype for integrated
aquaculture, whose capacity has been substantially increased.
We made some staff adjustments to the group in order to make it more coherent and focused.
During 2009 we published around 70 papers, including 37 in ISI journals, and 10 books or book
chapters and submitted several more.
In terms of main research results during 2009 we specifically:
65
-
Developed methodologies for comparability studies of littoral communities and
established precise data analysis procedures for biotope definition.
-
Evaluated the potential of seaweeds as bioindicators of heavy metals accumulation and
observed that Cystoseira abies-marina and Corallina elongata are good indicators for
polluted waters.
-
Evaluated plant/herbivore interactions and the effect of over-exploring herbivores in
the structure of intertidal communities and a negative relationship was found between
the cover of algae and limpets populations.
-
Tested the interactive effects of several environmental stressors on the metabolism and
growth of intertidal seaweeds.
-
Evaluated the processes operating in structuring marine Azorean communities and
concluded that the processes operating at the larger scale of islands played an
important role in structuring intertidal populations mainly on the lower algal dominated
communities.
-
Evaluated the impact of artificial sheltering of naturally exposed coasts and observed
these can promote a shift from consumer- to producer-dominated communities, with
important ecological and energetic consequences for the ecosystem.
-
Tested and confirmed the existence of counter-gradient latitudinal compensation in
growth of brown shrimp Crangon crangon and described its morphological variation
between populations across its geographic range of distribution in European waters,
confirming our previous finding at the genetic level.
-
Modelled the demography of the North Sea population C. crangon using the Dynamic
Energy Budget theory and contributing to a long discussion on the eggs’ origin of the
commercially exploited stock of the species; applying the same theory, we
reconstructed the food conditions for Northeast Atlantic bivalve species.
-
Described patterns in abundance and distribution of juvenile flounder, Platichthys
flesus, in Minho estuary.
-
Finalized the characterization of the productivity and capacity for nutrient uptake of
Gracilaria vermicolophyla in IMTA.
-
Selected seaweed species grown in IMTA systems as ingredients for fish feeding.
Gracilaria and Porphyra gave the best results for trout and tilapia feeding and continue
to be produced for the next round of longer term fish trials.
-
Tested an integrated approach of astaxanthin production from the microalgae
Haematococcus to determine their industrial feasibility using vertical and horizontal
photobioreactors placed outdoors in Azores at natural conditions and obtained biomass
values higher than previously reported, suggesting that the methodology applied is
effective and promising for an industrial production of astaxanthin.
66
In terms of outreach and involvement of young people in research we continued our program
“MOBIDIC: the school goes to the beach” for monitoring rocky shore communities with school
children and their teachers. This initiative started in 2005, involved more than 2000 students
from 12 local schools and 3 other groups and includes a public database with the results from
each survey. This year we organised an European workshop to promote this program in other
countries. We also had several internships for high school students, we held a summer program
and participated in several science fairs. We published a guide for the seashore in special waterproof paper. We participated in television programs on environmental issues (e.g. Biosfera).
In terms of linking research with policy makers and managers at different levels and promoting
policy relevant research, we continue to participate in the EPBRS, which met to discuss research
priorities for Europe and to produce a Research Strategy for the next 10 years. We also
negotiated with the Polis Litoral Norte and with the DRCT and SRAM from the Regional
Government of the Azores for funding research needed for regional coastal and resource
management.
8d. Productivity
1. Abreu, MH, Varela, DA, Henríquez, L, Villarroel, A, Yarish, C, Sousa-Pinto, I, Buschmann, AH.
2009. Traditional vs integrated multi-trophic aquaculture of Gracilaria chilensis. CJ Bird, J
McLachlan & EC Oliveira: productivity and physiological performance. Aquaculture 293: 211220. IF=1.925.
2. Araújo, R, Bárbara, I, Tibaldo, M, Berecibar, E, Díaz Tapia, P, Pereira, R, Santos, R, Sousa Pinto,
I. 2009. Checklist of benthic marine algae of Northern Portugal. Botanica Marina 52: 24-46.
IF=1.090.
3. Araújo, R, Vaselli, S, Almeida, M, Serrão, EA, Sousa-Pinto, I. 2009. Disturbance on marginal
populations: effects of human trampling on Ascophyllum nodosum assemblages at its southern
distribution limit. Marine Ecology Progress Series 378: 81-92. IF=2.519.
4. Arenas, F, Rey, F, Sousa-Pinto, I. 2009. Diversity effects beyond species richness: evidence
from intertidal macroalgal assemblages. Marine Ecology Progress Series 381: 99-108. IF=2.519.
5. Bird, G, Larsen, K. 2009. Tanaidacean Phylogeny - The second step: the basal Paratanaoidean
families (Crustacea: Malacostraca). Arthropod Systematics & Phylogeny 67(2): 137-158.
6. Campos, J, Freitas, V, Pedrosa, C, Guillot, R, Van der Veer, HW. 2009. Latitudinal variation in
growth of Crangon crangon (L.): does counter-gradient growth compensation occur? Journal of
Sea Research 62: 229-237. IF=1.803.
7. Campos, J, Pedrosa, C, Rodrigues, J, Santos, S, Witte, J, Santos, P, Veer, H. 2009. Population
zoogeography of brown shrimp Crangon crangon along its distributional range based on
morphometric characters. Journal of the Marine Biological Association of the United Kingdom
89(3): 499-507. IF=0.995.
67
8. Campos, J, van der Veer, HW, Freitas, V, Kooijman, SALM. 2009. Contribution of different
generations of the brown shrimp Crangon crangon (L.) in the Dutch Wadden Sea to commercial
fisheries: A dynamic energy budget approach. Journal of Sea Research 62: 106-113. IF=1.803.
9. Canning-Clode, J, Bellou, N, Kaufmann, MJ, Wahl, M. 2009. Local-regional richness
relationship in fouling assemblages - effects of succession. Basic and Applied Ecology 10: 745753. IF=2.422.
10. Doropoulos, C, Hyndes, GA, Lavery, PS, Tuya, F. 2009. Dietary preferences of two seagrass
inhabiting gastropods: Allochthonous vs autochthonous resources. Estuarine, Coastal and Shelf
Science 83: 13-18. IF=1.970.
2009. Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: A new sentinel
Comparative Biochemistry and Physiology C- Toxicology & Pharmacology 149(1): 58-64.
IF=2.582.
North-eastern Atlantic based on mitochondrial DNA. Marine Biology 156: 1421-1432. IF=1.999.
13. Freitas, V, Cardoso, JFMF, Santos, S, Campos, J, Drent, J, Saraiva, S, Witte, JIJ, Kooijman,
SALM, van der Veer, HW. 2009. Reconstruction of food conditions for Northeast Atlantic bivalve
species based on Dynamic Energy Budgets. Journal of Sea Research 62: 75-82. IF=1.803.
14. Freitas, V, Costa-Dias, S, Campos, J, Bio, A, Santos, P, Antunes, C. 2009. Patterns in
abundance and distribution of juvenile flounder, Platichthys flesus, in Minho estuary (NW
Iberian Peninsula). Aquatic Ecology 43(4): 1143-1153. IF=1.549.
15. León-Cisneros, K, Riosmena-Rodríguez, R, Neto, AI, Hernández-Carmona, G. 2009. The red
algal genus Scinaia (Nemaliales; Rhodophyta) on the Gulf of California, Mexico: a taxonomic
account. Phycologia 48(3): 186-210. IF=1.218.
16. Maggi, E, Bertocci, I, Vaselli, S, Benedetti-Cecchi, L. 2009. Effects of changes in number,
identity and abundance of habitat-forming species on assemblages of rocky seashores. Marine
Ecology Progress Series 381: 39-49. IF=2.519.
17. Martins, GM, Amaral, AF, Wallenstein, FM, Neto, AI. 2009. Influence of a breakwater on
nearby rocky intertidal community structure. Marine Environmental Research 67: 237-245.
IF=1.755.
18. Ortega, L, Tuya, F, Haroun, RJ. 2009. El erizo de mar Diadema antillarum Phillipi, 1845 influye
sobre la diversidad y composición de la comunidad de mega-invertebrados vágiles en fondos
rocosos del Archipiélago Canario. Revista de Biología Marina y Oceanografía 44(2): 489-495.
IF=0.361.
68
19. Pullin, A, Báldi, A, Can, O, Dieterich, M, Kati, V, Livoreil, B, Lövei, G, Nevin, O, Selva, N, SousaPinto, I. 2009. Conservation focus on Europe: major conservation policy issues that need to be
informed by conservation science. Conservation Biology 23: 818-824. IF=4.666.
20. Ramírez, R, Tuya, F, Haroun, RJ. 2009. Efectos potenciales del marisqueo sobre moluscos
gasterópodos de interés comercial (Osilinus spp. y Patella spp.) en el Archipiélago Canario.
Revista de Biología Marina y Oceanografía 44(3): 703-714. IF=0.361.
21. Rosas-Alquicira, EF, Neto, AI, Riosmena-Rodríguez, R, Couto, RP. 2009. New additions to the
Azorean algal flora, with ecological observations on rhodolith formations. Cahiers de Biologie
Marine 50: 143-151. IF=0.873.
Pollution 157: 2751-2758. IF=3.426.
75: 661-666. IF=3.253.
24. Silva, K, Almada, VC, Vieira, MN, Monteiro, NM. 2009. Female reproductive tactics in a sex
role reverser pipefish: screening for quality and number. Behavioural Ecology 20: 768-772.
IF=2.981.
25. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2009. Can the limited marsupium space be a
limiting factor for females? Insights from the black-striped pipefish, Syngnathus abaster. Journal
of Animal Ecology 77(2): 390-394. IF=3.714.
exposure to low levels of ethinylestradiol. Aquatic Toxicology 95: 330-338. IF=3.124.
27. Thomsen, MS, Wernberg, T, Tuya, F, Silliman, BR. 2009. Evidence for impacts of
nonindigenous macroalgae: a meta-analysis of experimental field studies. Journal of Phycology
45: 812-819. IF=2.270.
28. Tittley, I, Neto, AI, Parente, MI. 2009. The marine algal (seaweed) flora of the Azores:
additions and amendments 3. Botanica Marina 52: 7-14. IF=1.090.
29. Tuya, F, Haroun, RJ. 2009. Phytogeography of Lusitanian Macaronesia: biogeographic
affinities in species richness and assemblage composition. European Journal of Phycology 44:
405-413. IF=1.556.
30. Tuya, F, Wernberg, T, Thomsen, MS. 2009. Colonization of gastropods on subtidal reefs
depends on density in adjacent habitats, not disturbance regime or latitude. Journal of
Molluscan Studies 75: 27-33. IF=1.074.
69
31. Tuya, F, Wernberg, T, Thomsen, MS. 2009. Habitat structure affect abundances of labrid
fishes across temperate reefs in south-western Australia. Environmental Biology of Fishes 86:
311-319. IF=1.155.
32. Veiga, P, Rubal, M, Besteiro, C. 2009. Shallow sublittoral meiofauna communities and
sediment polycyclic aromatic hydrocarbons (PAHs) content on the Galician coast (NW Spain), six
months after the Prestige oil spill. Marine Pollution Bulletin 58: 581-588. IF=2.630.
33. Villanueva, RD, Hilliou, L, Sousa-Pinto, I. 2009. Postharvest culture in the dark: an ecofriendly alternative to alkali treatment for enhancing the gel quality of κ/ι-hybrid carrageenan
from Chondrus crispus (Gigartinales, Rhodophyta). Bioresource Technology 100: 2633-2638.
IF=4.253.
34. Wallenstein, FM, Couto, RP, Amaral, AS, Wilkinson, M, Neto, AI, Rodrigues, AS. 2009.
Baseline metal concentrations in marine algae from São Miguel (Azores) under different
ecological conditions - urban proximity and shallow water hydrothermal activity. Marine
Pollution Bulletin 58: 424-455. IF=2.630.
35. Wallenstein, FM, Terra, MR, Pombo, J, Neto, AI. 2009. Macroalgal turfs in the Azores. Marine
Ecology – An Evolutionary Prespective 30: 113-117. IF=1.558.
36. Wallenstein, FM, Torrão, DF, Neto, AI, Wilkinson, M, Rodrigues, A. 2009. Effect of exposure
time on the bioaccumulation of Cd, Mg, Mn and Zn in Cystoseira abies-marina samples subject
to shallow water hydrothermal activity in São Miguel (Azores). Marine Ecology 30: 118-122.
IF=1.558.
37. Wirtz, P, Canning-Clode, J. 2009. The invasive bryozoan Zoobotryon verticillatum has arrived
at Madeira Island. Aquatic Invasions 4: 669-670.
proceedings)
Books (Chapters)
1. McQuaid, C, Arenas, F. 2009. Biological Invasions: insights from marine benthic communities.
In: Wahl, M (Ed). Hard Bottom Communities: Patterns, Scales, Dynamics, Functions, Shifts.
Ecological Studies, Springer Verlag, Berlin, Germany, pp. 309-320.
2. Pereira, R, & Yarish C (In press). The role of Porphyra in sustainable culture systems:
Physiology and Applications. In: Israel, A, Einav, R, Seckbach, J (Eds). Seaweeds and their role in
globally changing environments.
Other Publications
3. Heip, C, Hummel, H, van Avesaath, P, Appeltans, W, Arvanitidis, C, Aspden, R, Austen, M,
Boero, F, Bouma, TJ, Boxshall, G, Buchholz, F, Crowe, T, Delaney, A, Deprez, T, Emblow, C, Feral,
JP, Gasol, JM, Gooday, A, Harder, J, Ianora, A, Kraberg, A, Mackenzie, B, Ojaveer, H, Paterson, D,
Rumohr, H, Schiedek, D, Sokolowski, A, Somerfield, P, Sousa Pinto, I, Vincx, M, Węsławski, JM,
Nash, R. 2009. Marine Biodiversity and Ecosystem Functioning. Printbase, Dublin, Ireland.
70
4. Neto, AI, Boero, F, Heip, C, Azevedo, JMN, Martins, G, Tittley, I, Wilkinson, M, Zacharias, M,
Rodrigues, AS (Eds.). 2009. Marine ecology and evolutionary perspective. Proceedings for the
43th European Marine Biology Symposium. Marine Ecology 30(S1). Wiley-Blackwell, Società
Italiana di Biologia Marina and AIOL (Associazione Italiana di Oceanologia e Limnologia)
Publishers.
5. Rubal, M, Veiga, P, Besteiro, C. 2009. Nematode/Copepod index: importance of sedimentary
parameters, sampling methodology and baseline values. Thalassas 25: 9-18.
proceedings)
1. Cabral, JP. 2009. Gonçalo Sampaio. Professor e Botânico Notável. Editora da Universidade do
Porto, Série Memória 1, Porto, Junho de 2009, 338 p. ISBN 978-972-8025-84-7.
2. Cabral, JP. 2009. Gonçalo Sampaio. Vida e Obra – Pensamento e Acção. Edição da Câmara
Municipal da Póvoa de Lanhoso, Póvoa de Lanhoso, Junho de 2009, 301 p. ISBN 972-96776-5-8.
3. Cabral, JP. 2009. Gonçalo Sampaio y el estudio moderno da la flora ibérica. Análisis de
manuscritos epistolares. Boletín de la Real Sociedad Española de Historia Natural (Sección
Biológica), 103(1-4): 9-26.
4. Neto, AI, Wallenstein, FM, Álvaro, NV, Azevedo, JMN. 2009. Guias Costeiros dos Açores: Ilha
Graciosa [Coastal Guides of the Azores: Graciosa Island]. Secretaria Regional do Ambiente e do
Mar e Câmara Municipal de Santa Cruz, Graciosa, Açores, Portugal.
5. Pereira, R. 2009. Seaweeds under the spotlight. Alga - Bulletin of the Spanish Phycological
Society 41: 13-15.
7. Tuya, F, Haroun, RJ. 2009. Biogeographic affinities in species richness and assemblage
composition across the lusitanian macaronesia. Alga - Bulletin of the Spanish Phycological
Society.
8. Veiga, P, Rubal, M, Besteiro, C. 2009. Primera cita de Orzeliscus belopus Du Bois-Reymond
Marcus, 1952 para la Península Ibérica, con notas sobre otros tardígrados encontrados en las
rías de O Barqueiro y Foz (Galicia, NO Península Ibérica). Boletín de la Real Sociedad Española de
Historia Natural 103: 59-65.
9. Wallenstein, FM, Neto, AI, Álvaro, NV, Tittley, I, Azevedo, JMN. 2009. Metodologias para
caracterização de biótopos e caracterização geral de comunidades costeiras em Ilhas Oceânicas
[Methodologies for Biotope Surveys and General Benthic Coastal Community Characterization in
Oceanic Islands]. Secretaria Regional do Ambiente e do Mar, Portugal.
71
MASTER THESES
Álvaro, NMV. 2009. Proposta de plano de monitorização para a área protegida para a gestão de
habitats ou espécies do Ilhéu de Vila Franca do Campo. Master thesis, University of Azores.
Supervisor: AI Neto, Co-supervisor: Fátima Alves (University of Aveiro). (Thesis submitted in
2009, defense in 2010)
Benitez, SR. 2009. Consecuencias de la diversidsd en la productividad de las comunidades de las
macroalgas. Master thesis in Coastal Management, University las Palmas de Gran Canaria.
Supervisor: F Arenas.
Coelho, DF. 2009. Protozoários na avaliação de ETAR. Caracterização do tanque de arejamento
com base no estudo da comunidade de protozoários. Master thesis in Biology, Faculty of
Sciences, University of Porto. Supervisor: ML Fidalgo, Co-supervisor: Ana Nicolau (University of
Minho).
Damião, V. 2009. Água da chuva que utilização? Master thesis in Biology and Water Quality
Management, Faculty of Sciences, University of Porto. Supervisor: MN Vieira.
Elias, CL. 2009. Utilização de diatomáceas na avaliação da qualidade do Rio Febros. Master
thesis in Biology and Water Quality Management, Faculty of Sciences, University of Porto.
Supervisor: MN Vieira.
Rocha, LC. 2009. Toxicidade de um efluente têxtil e de auxiliares têxteis em peixe zebra, Danio
rerio. Master in Hidrobiology, Faculty of Sciences, University of Porto. Supervisor: ML Fidalgo.
Silva, BL. 2009. Studies of cultivation of red and brown algae in the laboratory, for application in
integrated multitrophic aquaculture systems. Master thesis, ICBAS, University of Porto.
Supervisor: IS Pinto, Co-supervisor: R Pereira. (Thesis submitted in 2009, defense in 2010)
Silva, LMC. 2009. Qualidade biológica da água do rio Rabaçal. Master thesis in Biology and Water
Management, Faculty of Sciences, University of Porto. Supervisor: MN Vieira.
Vieira, RP. 2009. Estudo da dinâmica populacional de Grateloupia turuturu na costa portuguesa.
Master thesis in Costal Sciences, University of Aveiro. Supervisor: IS Pinto.
PhD THESES
Alquicira, EFR. 2009. Filogeografia de algas Corallinales (Rhodophyta) das Califórnias. PhD thesis,
University of Azores. Supervisor: AI Neto, Co-supervisor: Rafael Riosmena Rodríguez
(Universidad de Baja Califórnia Sur, México). (Thesis submitted in 2009, defense in 2010)
Campos, JCVB. 2009. The eco-geography of the brown shrimp Crangon crangon in Europe. PhD
thesis, Vrije Universiteit Amsterdam, The Netherlands. Supervisor: SALM Kooijman, Cosupervisor: Henk van der Veer (NIOZ, The Netherlands).
72
Cisneros, KL. 2009. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais. PhD
thesis, University of Azores. Supervisor: AI Neto, Co-supervisor: Rafael Riosmena Rodríguez
(Universidad de Baja Califórnia Sur, México). (Thesis submitted in 2009, defense in 2010)
-
2nd European Congress for Conservation Biology, 2-7 September, Prague, Czeck
Republic. IS Pinto - Member of the Scientific Committee.
-
43th European Marine Biology Symposium, 8-12 September, Porta Delgada, Portugal. AI
Neto - Chair of the Scientific Committee.
-
October, Porto, Portugal. IS Pinto - Member of the Scientific Committee.
http://www.ciimar.up.pt/biolief/
-
Spring School for an European Monitoring Network Meeting, MarBEF, 13-16 March,
Porto. IS Pinto, R Vieira – Members of the Organizing Committee.
-
MarBEF Workshop: On a European Monitoring Network 2009, 13-14 March, Porto,
Portugal. IS Pinto, R Vieira – Members of the Organizing Committee.
-
Workshop: O Mar e os seus Recursos, Ocupação Científica dos Jovens nas Férias, Ciência
Viva, 14-24 July, Porto, Portugal. IS Pinto, R Vieira, MP Silva – Members of the
Organizing Committee.
For 2010:
-
International Symposium FloraMac2010, 23-25 September, Ponta Delgada, Portugal. AI
Neto - Member of the Organizing and Scientific Committee.
During 2009 we started collaboration with the company “Seaweeds Solutions” to establish a
pilot scale project for large scale cultivations of seaweeds for production of bioethanol. We were
hired by a Swedish company to test anti-fouling paints to be used in boats. We continue to do
research in collaboration with the Aquaculture company Coelho e Castro where we have a IMTA
prototype in development since 2002.
Our group continued to work extensively with colleagues from Europe and around the world
within EU framework projects and steering, executive or management Committees of Biostrat,
(32 countries), NoE Marbef, including 56 partners, and Era-Net Biodiversa. We have also
73
participated in ENCORA, a European coastal network, and in Nagisa (CoML), an international
project for large-scale monitoring of coastal biodiversity, and several bi-lateral projects with
Spain including the Canaries, with the Netherlands, Norway, Chile, Brazil and USA that include
co-advisorship of students.
We wrote the final papers from the European scale experiments that were performed under
NoE Marbef. 5 papers were submitted for publication in Ecology, Ecology Letters, Marine
Ecology Progress Series and Hydrobiologia (published in Feb 2010).
We were also very active in writing proposal for the 7th Framework Program, EU. Six proposals
have been delivered in December 2009 and January 2010 and are currently under review.
We continue to participate and co-ordinate the project “ECClima: Managing Effects of Global
Climate on Estuarine Biodiversity and Productivity” in partnership with the Royal Netherlands
Institute of Sea Research (NIOZ) and Bergen University.
We participate in the Erasmus Mundus MSc in Marine Biodiversity and Conservation with
Universities from Belgium, Spain, Lithuania, Portugal, France and we are helping to preparing a
proposal for a PhD program (partner is CCIIMAR).
IS Pinto is member of the:
-
Editorial Board of the “Journal of Marine Biodiversity”, Springer; Study Group on
Biodiversity Science (SG BioDiv) from ICES; Working group on Marine Biodiversity
Change from GEO BON (GEO intergovernmental initiative).
-
Steering Committee of EPBRS (European Platform for Biodiversity Research Strategy);
European Synthesis Committee of the International Program “Census of Marine Life
(CoML)” and member of the “2020 Science Council” to plan the continuation of this
program after 2010, Board of Directors of the “Society for Conservation Biology”
Europe, Executive Board of MARS – European Network of Marine Research Institutes
and Stations and of the Steering Committee of the European Association Marbef + Marine Biodiversity and Ecosystem Functioning.
Participation in the meetings promoted by the North Regional Coordination and Development
Commission (CCDR-N), contributing to the elaboration of regional priorities for the sustainable
development of marine research in the North of Portugal and Galicia region that resulted in a
final report “Regional Agenda for the Sea”, published in February 2009.
Neto, AI. 2009. Estudo da viabilidade de produção da amêijoa Ruditapes decussatus (Linnaeus,
1758) em aquacultura intensiva. Dep Biologia Univ Açores. 28 pp.
Neto, AI. 2009. Unidade piloto de produção de óleo vegetal a partir de microalgas - uma solução
na produção de biocombustíveis. Rel Téc Finan Prog. Sec Reg Ciência Tecnol Equip, Dir Reg
Ciência Tecnol. 9 pp.
74
Neto, AI. et al. 2009. Qualidade de águas costeiras da Ilha Terceira (Açores) e proposta de
monitorização. Dep Biologia Univ Açores. iii + 50 pp + Anexos.
Neto AI, et al. 2009. Qualidade de águas de transição da Ilha de São Jorge e proposta de
monitorização. Dep Biologia Univ Açores. iii + 32 pp + Anexos.
Neto, AI et al. 2009. Qualidade de águas costeiras do Grupo Oriental do arquipélago dos Açores
e proposta de monitorização. Dep Biologia Univ Açores. iii + 70 pp + Anexos.
Neto, AI et al. 2009. Caracterização das Massas de Água Costeiras da Ilha Terceira:
Enquadramento e Metodologias de trabalho. Dep Biologia Univ Açores. 48 pp.
Neto, AI et al. 2009. Caracterização das Massas de Água Costeiras das Ilhas de Santa Maria e São
Miguel: Enquadramento e Metodologias de trabalho. Dep Biologia Univ Açores. 51 pp.
Neto, AI et al. 2009. Estudo da viabilidade de produção de Lapa (Patella e Haliotis) em
aquacultura intensiva: aclimatação às condições de cultivo e monitorização. Dep Biologia Univ
Açores. 13 pp.
Pereira Silva, M, Sousa Pinto, I. 2009. Portuguese national review on Taxonomy and Biodiversity.
As a contribution to the Czech EPBRS - European Platform for Biodiversity Research Strategy (1922 May 2009), Biostrat Project. CIIMAR Porto Portugal.
Pereira Silva, M, Sousa Pinto, I. 2009. State of the Biodiversity National Platform - 2009. Biostrat
Project. CIIMAR Porto Portugal.
Pereira Silva, M, Sousa Pinto, I. 2009. Taxonomy and Biodiversity - European overview. As a
contribution to the Czech EPBRS - European Platform for Biodiversity Research Strategy (19-22
May 2009), Biostrat Project. CIIMAR Porto Portugal.
Santos, P. 2009. Colaboração no Plano de Ordenamento da Albufeira do Alto Rabagão. Cliente
Quaternaire-Portugal.
8e. Future research
1. Objectives
The general objectives of the group will continue to be the study of different aspects of marine
biodiversity and its role on coastal aquatic ecosystems, with the final goal of not only advancing
science but also effectively conveying the scientific knowledge to policy makers and managers in
order to promote a sustainable use of marine resources. We will further integrate our research
with other groups at CIIMAR, national and international level, and we will strengthen our
participation in teams from different disciplines in order to do more interdisciplinary research.
This process has already started with our participation to a consortium that is applying for the
FP7 project: “ARISE - Adaptive Response of Integrated Social, Economic & Ecological Systems to
vectors of change” that is currently under evaluation. We will continue to increase our inputs to
global databases (Algabase, GBIF, OBIS), international research programs (CoML and its
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successor 2020, Geo Bon) and national initiatives such as the network for Marine and Coastal
Biodiversity that we founded in 2007 and national projects such as EEMA.
The integration of new members with very high taxonomic skills and with genetic background
will greatly increase our ability to identify and quantify the biodiversity that is present in our
waters, as well as to contribute to the global development of taxonomy, especially of marine
crustaceans (Tanaidacea) and different groups of macroalgae.
The integration of a new member with modelling capabilities will allow developing and
implementing hydrodynamic and biogeochemical models, to integrate knowledge on coastal
ecosystems and develop decision support systems that can be used as management tools.
We also aim to provide relevant data and knowledge to the implementation of environmental
and marine policies, including the EU Water Framework Directive (project EEMA) and the
Habitats Directive for the marine environment, and we will contribute to the development of
methodologies for the implementation of other EU Directives, such as the Marine Strategy
Directive and ICZM. We will start a project funded by a regional program to characterize the
main habitats and ecosystems from the northern coastal zone and the main artisanal fishing
activities in this area. The results from these studies will be used to improve coastal
management activities in north Portugal. We will also continue to collaborate with the regional
government of Azores to improve knowledge relevant for policy making and management. We
will continue to carry out research and develop models that can implement management of
marine resources, mitigation and adaptation measures to climate change and to biological
invasions.
Within this framework we will work further on:
-
Patterns of distribution and abundance of benthic species across their geographic range
of distribution in Europe, and effects of climate change (Sandalga, CLEF, Physiography
and ECClima projects), particularly focusing on subtidal and estuarine assemblages and
on the phylogeography of selected species, including algae and lobsters). We will further
investigate the effects of gene flow across a range of spatial scales in the Azorean
archipelago: within islands, among islands and among groups of islands.
-
Description of patterns of abundance and size structure of mobile fauna in subtidal
habitats, particularly fauna associated with kelp holdfasts.
-
Trophic links and estuarine food web structure and dynamics, within the ECClima
project.
-
Effects of several abiotic and biological stressors, including sedimentation, temperature
changes and invasive species, on benthic communities.
-
Recording the introduction, demography and effects of exotic and invasive species in
order to evaluate the need for management actions; we will start a survey of ports and
marinas to detect species that have been likely introduced by boats and to evaluate
their potential for becoming invasive (INVASEA, CLEF and AXA projects).
76
-
Completing a catalogue of the Portuguese fauna of selected micro-crustacean taxa, with
particular emphasis on the characterization of biodiversity change in soft-bottom
coastal ecosystems in response to natural forces, local policies and global change.
-
Improving the systematics and phylogeography of micro-crustacean, specially from
deep-sea habitats and of macroalgal taxa and combining molecular and morphological
phylogenies
-
Continue the development of new products from marine organisms, including the algae
cultivated in IMTA systems, for the production of biofuels (project in negotiation with
private company), biomedical compounds (ongoing PhD and IBEROMARE projects),
cosmetic products (ongoing PhD), antibacterian agents (several graduation projects,
IBEROMARE) and ingredients for fish feeding (Benefits and Post-doctoral project).
-
Continue to work with schools and Ciência Viva organization to promote education on
marine biodiversity and ecosystems, their importance and need for conservation
(MOBIDIC, internships, Summer programs and fairs, other).
-
Continue to work with policy makers and managers, especially at regional and European
level, to promote policy relevant research and the use of research results in coastal and
resource management and policies.
Several projects have already been approved and due to start in 2010:
-
BENEFITS - Benefícios da produção de macroalgas marinhas em sistemas de aquacultura
integrada multitrófica.
-
HYSIOGRAPHY - Physiological stress of intertidal fucoids related to their biogeography:
implications under new climate scenarios.
-
NVASEA - Shifts from seagrass to seaweed dominated system.
-
CARRAGEENAN - Unraveling the interplay between the microstructure and the
mechanical properties in kappa/iota-hybrid carrageenan gels.
-
CleF - The combined impacts of invasion and climate change on coastal ecosystem
functioning.
-
CHRACTER Characterization of the marine coastal habitats and the artisanal fishing
practices of the Natura 2000 site Sítio Litoral Norte.

Current funding
12.000 EUR, PhD grants.
77
-
FCT, 2010-2013
121.000 EUR, BENEFITS.
117.000 EUR, PHYSIOGRAPHY.
68.000 EUR, INVASEA.
70.0
R, CARRAGEENAN.
70.000 EUR, CleF.
-
FP6, 2006-2010
203.000 EUR, BIODIVERSA
148.000 EUR, BIOSTRAT.
-
Other
1.000.000 EUR, EEA, 2007-2011.
158.000 EUR, QREN, 2009-2011.
57.000 EUR, Axa Foundation, 2008-2011.
82.000 EUR, INTERREG III, 2009-2012.
306.000EUR, DRCT, 2008-2010.
94.000 EUR, SRAM, 2007-2010.
220.000 EUR, CHRACTER, 2010-2011.

Pending funding (under evaluation)
-
FCT
158.000 EUR, Phylogeography of the Macaronesia NE Atlantic Mediterranean slipper lobsters.
145.000 EUR, BIOMEASURE - A biodiversidade como uma medida estado do habitat:
identificação de organismos bioindicadores para detectar o impacto da eutrofização causada
pelo Homem.
160.000 EUR, PatelGene - Estrutura Genética das Lapas no Arquipélago dos Açores: Implicações
para a conservação e designação de áreas marinhas protegidas.
71.000 EUR, RAP - Responses to Anthropogenic Perturbations: climatic and nutrient effects on
rock pool assemblages.
78
83.000 EUR, Last but not least: uncovering the biodiversity within subtidal tanaids, using
molecular and morphological data.
135.000 EUR, Effects of ocean climate on the resilience of kelp beds across continental Portugal.
-
FP7, EC
200.000 EUR, ARISE - Adaptive Response of Integrated Social, Economic & Ecological Systems to
vectors of change.
62.000 EUR, Euromarine - Integration of European Marine Research Networks of Excellence.
80.000 EUR, NEU - Developing a Knowledge Network for EUropean expertise on biodiversity and
ecosystem services to inform policy making and economic sectors.
485.000 EUR, EMERSE - European Marine Education through Research.
BIOLINK - BIOLogical Integrated Knowlewdge into ERA and Outermost Regions.
EMBOS - Development and implementation of a pan-European Marine Biodiversity Observatory
System.
79
Biogeographical Ecology and Evolution (BEE)
Biogeographical Ecology and Evolution
Maria Ester Tavares Alvares Serrao
Amount shown is only that for BEE Research Group:
-
FCT pluriannual funding
-
EU funding
190.672 EUR, FP7-ENV-2008-1-226248, Arctic Tipping Points, 2009-2012.
891.160 EUR, LIFE 06 NAT/P/000192, BIOMARES, 2007-2010.
295.908 EUR, NEST-2005-Path-COM/043251, EDEN, 2007-2009.
32.500 EUR, ESF-EUROCORES 1390, DEECON, 2005-2009.
-
FCT National funding
As coordinators:
190.672 EUR, PTDC/MR/72630/2006, SOPA, 2008-2011.
191.441 EUR, PTDC/MAR/65461/2006, MEGIKELP, 2008-2011.
187.140 EUR, PTDC/MAR/64749/2006, IBISA, 2008-2011.
182.630 EUR, PTDC/MAR/70921/2006, CAULERPA, 2008-2011.
86.400 EUR, POCI/MAR/61105/2004, ADAPT, 2006-2009.
82.800 EUR, POCI/MAR/60179/2004, DIVSTAB, 2006-2009.
80
89.100 EUR, POCI/MAR/57499/2004, MATING, 2006-2009.
Plus 7 new projects approved in 2009 (1.238.065 EUR) to start in 2010.
As partners:
25.020 EUR, POCI/MAR/56149/2004, LIMITS, 2006-2009.
35.138 EUR, PTDC/BIA-BDE/74349/2006, HERCULES, 2008-2011.
53.648 EUR, PTDC/BIA-BDE/68730/2006, METAPOP, 2008-2010.
78.000 EUR, MarinERA 189570, SHIFTING, 2008-2011.
-
Subcontracts
30.000 EUR, ECOKELP, ANR, France, 2007-2009.
8.500 EUR, ICCM, Spain, 2006-2009.
-
Other
3.200 EUR, FINDKELP, AWARE Foundation.
1. Objectives
We aim at understanding patterns and processes mediating population biology from ecological
to evolutionary scales. Topics include phylogeography, population dispersal/connectivity, abiotic
stress-driven evolution, reproductive ecology and roles of mating systems in population
divergence and speciation. Biological models include marine plants, algae, animals, using a
variety of approaches, from molecular to experimental ecology and phylogenetic reconstruction.
A large focus of current research is the central question in evolutionary ecology of the nature of
environmental barriers that limit gene flow and induce population genetic divergence, a first
step towards speciation. We identify gene flow barriers in marine populations, a subject
particularly poorly understood for marine taxa given the apparent continuity of marine
environments for dispersal, and we track genetic imprints of ocean-climate cycles (ice,
temperatures and sea levels) from Pleistocene ice ages, on several taxa of pelagic and benthic
marine species across various oceans and seas, inferred from genetic structure and molecular
phylogenies. We look for imprints of historical climate change on retreating and leading edge
populations of cold temperate and warm water species, focusing on key canopy-forming habitat
structuring species and associated animal taxa.
In biogeographical theory we empirically test theoretical predictions from evolutionary ecology
of species´ranges, whereby distributional edges and marginal habitats would be expected to
have higher clonality and inbreeding, selected for reproductive assurance and local adaptation,
81
but also higher genetic differentiation and lower genetic diversity which trade-off with higher
selective pressures for stress-driven local adaptation at range edges, reducing their evolvability.
We address leading questions in marine ecology, such as maximizing fertilization success in
exposed shores by timing broadcast spawning synchrony, comparing population mating systems
in order to understand their roles in enabling species to respond to variable conditions and
eventually driving reproductive isolation and speciation, maintaining species integrity in sister
taxa that can hybridize and introgress.
We focus particular research objectives on ecosystems that play important roles in our planet
for global climate and ocean productivity, their extreme conditions for life, or for sustaining
marine biodiversity, such as polar phytoplankton, deep sea vents, kelp forests and seagrass
meadows, in addition to species that play a negative role as invasives:
In polar oceans, which disproportionately influence global climate, we characterize genomewide patterns of stress-responsive gene expression in natural phytoplanktonic assemblages. In
polar research cruises we experimentally manipulate natural phytoplanktonic assemblages for
major hypothesized drivers of ecosystem function (temperature, UV) and we study
transcriptomic responses, using next generation high-throughput pyrosequencing of cDNA
libraries.
In the deep sea, we study phylogeny and phylogeography of deep-sea animals, particularly from
fragmented and short-lived chemosynthetic ecosystems, to trace back colonization pathways
along mid-ocean ridges.
In giant kelp forests, that sustain one of the most productive and dynamic ecosystems on the
planet, we unravel gene-flow patterns at different spatial scales, from intra- to metapopulation
scales in California, to global ocean biogeography. We map and estimate gene flow within and
between patches of Iberian kelp forests and seagrass meadows to infer population
fragmentation and connectivity.
To track the evolution of invasive Caulerpa species and their associated bacterial flora, we
compare Australian (native range) and Mediterranean (invaded range) algal-bacterial
associations using metagenomics.
We put effort into contributing to society awareness and to conservation of marine ecosystems,
by developing projects aimed at marine habitat conservation and restoration at a Marine
Protected Area, and using volunteer public participation and various public communication
means.
All these objectives require significant initial effort into methodological objectives, including
development of various types of molecular methods and markers, novel population genetics
analyses methods, and developing specific software.
22 papers and 1 book chapter in 2009. Major findings:
Phylogeography, Phylogeny, Genome evolution:
82
-
We fully sequenced complete mt genomes of 7 Neogastropoda (predatory marine
snails) and a sister group, revealing that Neogastropoda are not monophyletic,
challenging morphological evidence and calling for re-evaluation of morphological
synapomorphies.
-
We fully-sequenced chloroplast genomes of two brown algae, and inferred their
rearrangements when compared with other red-algal derived plastids. Our phylogeny
across all chromist plastids also supported the monophyly of heterokont plastids and
that of cryptophyte and haptophyte plastids.
-
Based on mt and nuclear phylogenies we discovered that two sympatric fish species in
the genus Macrorhamphosus are morphotypes of a single genetic entity.
-
We inferred the phylogeography of the sand smelt, Atherina presbyter, showing higher
genetic diversity in southern European populations that likely acted as refugial source
for postglacial colonization of the North.
Genetic recovery from ecosystem disturbance:
-
We reconstructed the evolution of genetic diversity of the mangrove tree Avicennia alba
over time since the local extinction of mangrove forests of the Mekong Delta, Vietnam,
by the spread of Agent Orange by US forces. Three decades following the end of the
war, genetic diversity is still increasing, revealing that genetic recovery may continue
over time scales that are much longer than demographic recovery.
Landscape genetics - kelp, seagrasses and coastal lagoon ecosystems:
-
Using kelp forest mapping and molecular markers we quantified the dependence of
gene flow on both distance and habitat continuity (landscape effects) and modeled the
combination of effective population size and mean dispersal distance that explain
genetic differentiation among giant kelp beds in California.
-
As a first step towards landscape genetics of Portuguese kelp and seagrasses, we GISmapped the kelp forests and seagrass meadows of Portugal, based on aerial
photography (seagrasses), volunteer geo-referenced reports (kelp) and ground-truthing
cross-surveys (for all), achieving perfect agreement level in the concordance scale. In
doing this we also raised public education and awareness of seagrass and kelp bed
importance.
-
Genetic variability of cockles (Cerastoderma glaucum) inside a costal lagoon (Mar
Menor, Spain) was correlated with environmental conditions, particularly salinity and
sediment type, reflecting also the main circulation patterns in the lagoon.
Edge populations – adaptive capacity and resilience:
-
Despite stronger selective pressures for local adaptation, small low diversity edge
populations can have lower adaptive capacity relative to larger and more genetically
diverse central ones; southern marginal populations of an intertidal brown alga (Fucus
83
serratus) showed reduced fitness to heat stress and their local habitat is reduced by
abiotic stressors.
-
We demonstrated the vulnerability of southern edge populations of the brown alga
Ascophyllum nodosum to disturbance, supporting evidence for increased risks of local
extinction at the boundaries of geographical distribution ranges.
-
Near the southernmost edge of the intertidal seagrass Zostera noltii, temperature
maxima recorded in natural populations in the summer at low tide are near the sublethal temperature for this species, raising concerns as to the future viability of these
marginal populations.
Reproductive ecology and larval retention:
-
We reviewed the evidence and controversies about fertilization strategies, concluding
that both sperm limitation and sperm competition play essential roles in driving the
ecology and evolution of marine fertilization.
-
A self-compatible hermahroditic fucoid alga, the invasive species Sargassum muticum,
uses tidal cues and local factors to time gamete and embryo embryo release, resulting in
differences in release patterns between different intertidal heights.
-
We described temporal variability of species and stages of marine reef fish larvae near
shore along temperate rocky reefs inside a Marine Park, indicating larval retention near
the reefs in this MPA.
Technical developments:
-
Software to analyse microsatellite allele binning.
-
Microsatellite genetic markers for giant kelp Macrocystis pyrifera, the seahorse
Hippocampus hippocampus, the pipefish Syngnathus abaster, the cavehopper
Palmorchestia hypogaea, the temporary lagoon amphibians Pelobates cultripes,
Pleurodeles waltl and Pelodytes punctatus for metapopulation genetics.
8d. Productivity
1. Alberto, F, Whitmer, A, Coelho, A, Zippay, M, Varela-Alvarez, E, Raimondi, PT, Reed, D, Serrão,
EA. 2009. Microsatellite markers for the giant kelp Macrocystis pyrifera. Conservation Genetics
10: 1915-191. IF=1.849.
2. Alberto, F. 2009. MsatAllele_1.0: an R package to visualize the binning of microsatellite alleles
Journal of Heredity 100(3): 394-397. IF=2.052.
84
3. Assis, J, Tavares, D, Tavares, JT, Cunha, AH, Alberto, FA, Serrão, EA. 2009. Findkelp, a GISbased community participation project to assess Portuguese kelp conservation status. Journal of
Coastal Research SI 56: 1469-1473. IF= 1.366.
5. Arnaud-Haond, S, Teixeira, S, Terrados, J, Tri, NH, Hong, NP, Duarte, CM, Serrao, EA. 2009.
Genetic diversity of mangrove Avicennia alba three decades after Agent Orange. Marine Ecology
Progress Series 390: 129-135. IF=2.519.
6. Borges, R, Vaz, J, Serrão, EA, Gonçalves, EJ. 2009. Short-term temporal fluctuation of verynearshore larval fish assemblages at the Arrábida Marine Park. Journal of Coastal Research SI 56:
376-380. IF=1.366.
7. Cunha, AH, Assis, J, Serrão, EA. 2009. Estimation of available seagrass meadow area in
Portugal for transplanting purposes. Journal of Coastal Research SI 56: 1100-1104. IF= 1.366.
8. Cunha, RL, Grande, C, Zardoya, R. 2009. Neogastropod phylogenetic relationships based on
entire mitochondrial genomes. BMC Evolutionary Biology 9: 210. IF=4.294.
9. Diekmann, OE, Gouveia, L, Serrão, EA, Van de Vliet, MS. 2009. Highly polymorphic
microsatellite markers for the black striped pipefish, Syngnathus abaster. Molecular Ecology
Resources 9(6): 1460-1466. IF= 1.251.
11. Gonzaléz-Wanguemert, M, Cánovas, F, Marcos, C, Pérez-Ruzafa, A. 2009. Phosphoglucose
isomerase variability of Cerastoderma glaucum as a model for testing the influence of
environmental conditions and dispersal patterns through quantitative ecology approaches.
Biochemical Systematics and Ecology 37: 325-333. IF=1.131.
derived plastids. BMC Evolutionary Biology 9: 253. IF=4.294.
13. Massa, SI, Arnaud-Haond, S, Pearson, GA, Serrão, EA. 2009. Temperature tolerance and
survival of intertidal populations of the seagrass Zostera noltii (Hornemann.) in Southern Europe
(Ria Formosa, Portugal) Hydrobiologia 619: 195-201. IF=1.754.
14. Monteiro, C, Engelen, AH, Serrão, EA, Santos, R. 2009. Habitat differences in the timing of
reproduction of the invasive alga Sargassum muticum (Phaeophyta, Sargassaceae) over tidal and
lunar cycles. Journal of Phycology 45: 1-7. IF=2.270.
85
15. Pearson, GA, Lago-Leston, A, Mota, C. 2009. Frayed at the edges: Selective pressure and
adaptive response to abiotic stressors are mismatched in low diversity edge populations. Journal
of Ecology 97: 450-462. IF=4.690.
16. Robalo, JI, Sousa-Santos, C, Cabral, H, Castilho, R, Almada, VC. 2009. Genetic evidence fails to
discriminate between Macroramphosus gracilis Lowe, 1839 and M. scolopax Linnaeus, 1758 in
Portuguese waters. Marine Biology 156 (8): 1733-1737. IF= 1.999.
17. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2009. Development and
characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad,
Pelobates cultripes. Conservation Genetics 10: 993-996. IF=1.849.
18. Van de Vliet, MS, Diekmann, OE, Serrão, EA, Beja, P. 2009. Isolation of highly polymorphic
microsatellite loci for a species with a large genome size: Sharp-ribbed Salamander (Pleurodeles
waltl). Molecular Ecology Resources 9: 425-428. IF=1.251.
19. Van de Vliet, MS, Diekmann, OE, Serrão, ETA. 2009. Highly polymorphic microsatellite
markers for the short-snouted seahorse (Hippocampus hippocampus), including markers from a
closely related species the long-snouted seahorse (Hippocampus guttulatus) Conservation
Genetics Resources 1: 93-96.
20. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2009. Highly polymorphic
microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for
cross-species amplification. Conservation Genetics 10: 665-668. IF=1.849.
21. Villacorta, C, Cánovas, F, Oromí, P, Juan, C. 2009. Isolation and characterization of
microsatellite loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae).
Conservation Genetics Resources 1: 401-404
proceedings)
Book (Chapter)
1. Serrão, EA, Havenhand, J. 2009. Fertilization strategies. In: Wahl, M (Ed). Marine Hard Bottom
Communities, Chapter 10. Ecological Studies 206, Springer Verlag. Berlin, Heidelberg. Doi:
10.1007/978-3-540-92704-4_10.
proceedings)
86
MASTER THESES
Costa, JF. 2009. Spiraling among the Fucus: the strange story of Fucus spiralis. Master thesis in
Marine Biology, University of Algarve. Co-supervisors: E Serrão, G Pearson, Jeanine Olsen (Univ.
Groningen).
Lima, C. 2009. Efeitos de herbivoria na restauração de pradarias de ervas marinhas. Master
thesis in Marine Biology, University of Algarve. Co-supervisors: E Serrão, J Gonçalves, A Cunha.
Lopes, EP. 2009. Filogeografia de Brachidontes puniceus (Gmelin, 1791) no arquipélago de Cabo
Verde. Master thesis in Marine Biology, University of Algarve. Supervisor: R Castilho.
Medeiros, JV. 2009. Influência de marés na distribuição vertical de larvas de peixe de recifes
costeiros e escolha de substratos de assentamento no Parque Marinho Luíz Saldanha. Master
thesis in Marine Biology, University of Algarve. Co-supervisors: R Borges, E Serrão, Emanuel
Gonçalves (ISPA).
Most of our graduate students have co-supervisors that are international specialists in the thesis
topics and the students spend some periods working and training in their laboratories.
We received visiting graduate students from other countries (2 from Spain, 1 from France, 1
from the USA, 1 from New Zealand) that asked to come to our laboratory to learn techniques
and conduct specific research for parts of their theses.
Approximately half of our publications are in co-authorship with international teams.
We recurrently receive EU funding from participation in international research projects.
Thanks to international networking collaborations with various international teams we are able
to have access to remote sampling, such as conducting research in Arctic and Antarctic research
cruises, to participate in sampling campaigns along the Pacific coastlines, to obtain animal
samples from deep sea vents.
87
We have participated in various international networks of excellence, Marine Genomics Europe,
MARBEF, CORONA (NSF, USA), and are core partners in the international project for genome
sequencing of the seagrass Zostera marina.
8e. Future research
1. Objectives
Besides continuation of the long-term research objectives already outlined in the section
“Objectives” above, to avoid repetition we detail here only the research objectives in our newly
funded projects:
-
We will address the major problem of species persistence under climate variability,
using genetic markers to trace back the effects of thermal oscillations along
distributional edges on population persistence and stability. Despite compelling
evidence of the impact of Northern hemisphere glacial periods in northern range
contractions and the role of glacial refugia in northern expansions, such climatic effects
at the southern range and past interglacial northern refugia have remained mostly
ignored in the interpretation of patterns of genetic diversity. We will compare across
species the role of the poorly understood southern rear edge interglacial range
contractions, expected for at least species with restricted thermal tolerances. We will
compare the location of the high genetic diversity centers within the range distributions
of multiple species with different thermal tolerances. Our previous work has revealed
genetic signatures of past climatic shifts on species distributional margins. Here we will
now move a step forward by applying information on ecological niche requirements of
temperature for different species, to understand climatic effects on persistence, and
consequent population genetic signatures, at distributional margins versus core centers.
-
The relationship between genetic diversity and fitness related traits in peripheral versus
central populations is a key question that we directly address using intertidal algae as
models to test the relationship between population history (as it affects genetic
variation) and adaptive capacity, or evolvability, which may be lowest in marginal
populations that are most likely to experience rapid and severe environmental change.
Our previous effort into development of genetic and genomic tools in the Fucus model
system, now allows us to directly test the relationship between diversity and fitnessrelated trait means at range centre and margins, characterize local adaptation along
steep gradients of environmental stress, including identification of candidate genes, and
analyse how the capacity and extent of local adaptation along steep local selective
gradients varies as a function of genetic diversity in populations across species ranges.
-
We will address the problem of coexistence of ecologically and phylogenetically similar
species despite incomplete reproductive isolation. This remains a most controversial
scientific problem in the understanding of sympatric speciation for broadcast spawning
88
marine taxa, a common and ancestral reproductive mode in the sea, with unique
challenges for the evolution of reproductive barriers. This will be elucidated using as
model taxa Fucus spp., for which our team has invested the last decade in developing
the expertise and tools required for these objectives: understand what pre-mating,
post-mating, and post-zygotic processes may be acting as reproductive barriers to interbreeding between sister species, and between sympatric but genetically differentiated
intra-species entities.
-
Polyandry, the fertilization of a female’s gametes by several male donors, influences the
genetic variability of a population, the reproductive success of males and females, and
individual fitness. Despite much evidence for polyandry in natural populations,
comparable data in external fertilizers is lacking. We will use as model the seaweed
Fucus vesiculosus, for which enough highly polymorphic genetic markers have been
generated that allow development of these objectives: estimation of polyandry,
reproductive success of males and females and potential for sexual selection, male-male
competition, the scope for female choice, and testing environmental factors that may
influence paternity shares, the distance between individuals and the synchrony of
gamete release.
-
Porphyra (Nori), the most economically important product in world aquaculture, will be
used as model in genetic approaches to understand the relative contribution of sexual
and asexual reproduction for population maintenance and the evolution of sympatric
congeneric species, using the 6 Porphyra spp. recorded in the Iberian peninsula.
-
We will use an innovative combination of seascape genetics and modeling approaches
for the seagrass Z. noltii inside the Ria Formosa lagoon to combine available GIS
seagrass cover maps with layers on seagrass genotypic richness and genetic
differentiation. This empirical approach will be complemented by a modeling approach
to estimate Z. noltii passive dispersal and predict space occupation. By combining
species niche modeling with spatial explicit genetic diversity information, we will add a
predictive value beyond the common analytical approach of landscape genetics, to
detect landscape barriers to gene flow, assess the environmental dependence of
allocation strategies (sexual versus asexual population growth) and understand putative
source-sink population dynamics.
-
We will investigate how the disturbance gradient associated with depth distribution
affects sexual allocation strategies for clonal marine plants, by combining a genetic
study with demographic analyses. The model will be the seagrass Cymodocea nodosa in
the Canary archipelago, which contains appropriate environmental gradients to study
sexual allocation strategies in these clonal organisms, from shallow hydrodynamic
disturbed to deep light limited stressed conditions.
-
Species invasions are natural experiments that enable the examination of ecological and
evolutionary processes in real time We will use invasive species as a model system for
rapid evolution in action, in comparison with indigenous species at a different
evolutionary stage. We aim to test the abundant-centre assumption by investigating
genetic diversity, behavioural and physiological traits of a recent invader and a similar,
89
coexisting native species at the margins and centre of their natural distributional range,
and in the invaded and native localities.

Current funding
-
FCT Pluriannual funding
-
EU funding
190.672 EUR, FP7-ENV-2008-1-226248, Arctic Tipping Points, 2009-2012.
891.160 EUR, LIFE 06 NAT/P/000192, BIOMARES, 2007-2010.
-
FCT funding
As coordinators:
182.328 EUR, FCT PTDC/AAC-CLI/109108/2008, 2010-2012.
199.810 EUR, PTDC/MAR/099698/2008, PORPHYRA, 2010-2012.
198.240 EUR, PTDC/MAR/108105/2008, HYBRID, 2010-2012.
141.370 EUR, PTDC/BIA-BEC/103916/2008, PERMYT, 2010-2012.
185.410 EUR, PTDC/MAR/104477/2008, PLOYANDRY, 2010-2012.
173.073 EUR, PTDC/MAR/099887/2008, RiaScapeGen, 2010-2012.
157.834 EUR, PTDC/MAR/108013/2008, CANARYGRASS, 2010-2012.
190.672 EUR, PTDC/MR/72630/2006, SOPA, 2008-2011.
191.441 EUR, PTDC/MAR/65461/2006, MEGIKELP, 2008-2011.
187.140 EUR, PTDC/MAR/64749/2006, IBISA, 2008-2011.
182.630 EUR, PTDC/MAR/70921/2006, CAULERPA, 2008-2011.
As partners:
35.138 EUR, PTDC/BIA-BDE/74349/2006, HERCULES, 2008-2011.
53.648 EUR, PTDC/BIA-BDE/68730/2006, METAPOP, 2008-2010.
78.000 EUR, MarinERA 189570, SHIFTING, 2008-2011.
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Biophysics
Biophysics
Maria Leonor Nunes Ribeiro Cruzeiro
FIS2009-06585-E/FIS, Transporte mediado por solitones en sistemas anarmonicos mono- y bidimensionales, Modalida: EF- EXPLORA. Total funding: 40.000 EUR, from Ministerio de Ciencia e
Innovacion, Spain. Coordinator: Manuel Garcia Velarde, Universidade Complutense de Madrid.
10.000 CPU hours, Estabilidade dinâmica do prião humano, in the supercomputer Milipeia of the
Laboratory for Advanced Computing of the University of Coimbra. PI: L Cruzeiro.
ca 23.340 EUR per annum, Postdoctoral grant for Dr. Holly Freedman, funded by FCT, ref.
SFRH/BPD/41143/2007. Supervisor: L Cruzeiro.
1. Objectives
The ultimate aim of the Biophysics Group is to understand how proteins fold and also how they
function, something that is not only of interest for Marine Biology but also for Biology in
general, as well as for Biomedicine, for Biotechnology and for the Pharmaceutical Sciences.
Our scientific approach is based on two fundamental assumptions: a) the free energy landscape
of proteins is multi-funnel shaped and b) protein folding and function involve the storing and
propagation of energy in the form of vibrational excited states (VES), something that we have
started to designate as the VES hypothesis. The model we use is the Davyodv/Scott in which the
vibrational state is the amide I vibration, but the NH stretch, which is two times more energetic,
may also be important since its energy corresponds to the total energy released in the
hydrolysis of ATP.
91
In 2009, three particular objectives were pursued: 1) classical dynamical simulations to test
assumption a) above, 2) calculations of the vibrational spectrum of the organic crystal of
acetanilide (ACN) in order to compare with experimental measurements, and, 3) code
modifications of the GROMACS package for classical protein dynamics to include the effect of
the quantum amide I vibrational excitations.
A fourth objective was the continuation of the collaboration with Prof. Manuel Garcia Velarde,
Madrid, Spain, both in the topic of electron-lattice interactions and in the new topic of electron
transfer.
Finally, as fifth objective, we have also started to think about practical applications of the
research developed (see item Patents/propotypes).
Concerning objective 1) above, in collaboration with Dr. Paulo Afonso Lopes and Dr. Pedro
Medeiros, of CITI, UNL, Lisbon, 100 ns simulations were run on four proteins which confirmed
the results already obtained for shorter simulations (Cruzeiro, L. 2009. Journal of Biological
Physics 35: 43-55), which already indicated that the same amino acid sequences can have
structures that are very different from the native and yet have the same thermodynamic
stability. A preliminary analysis of these longer simulations can be found in one of the
publications (Cruzeiro, L. in press. Protein Folding, Specialist Periodic Reports of the Royal
Society of Chemistry) where a new kinetic mechanism for protein folding is also put forward.
Concerning objective 2) above, a crystal of ACN with 864 was built from the known ACN
structure and its crystal symmetry group and molecular dynamics simulations were run on this
system at three different temperatures. The dipole-dipole amide I coupling matrix between
different ACN molecules were obtained using both classical dipole-dipole interactions and also a
quantum transition charge approach. The amide I spectra of the crystal were calculated using
these two kinds of coupling matrices, and compared to those known experimentally. Our results
confirmed the experimental prediction that localization is most important for the lower-energy
absorption peak. Moreover, when we employed the classical transition dipole coupling
approach, we obtained 2 temperature-dependent peaks, as seen experimentally, at roughly the
right energy separation.
Concerning objective 3) above, an additional term has been added to GROMACS non-bonded
forces on the carbon, oxygen, nitrogen and hydrogen atoms in the amide groups of proteins, in
order to describe the influence that a quantum vibrational excitation (an amide I excitation) can
have on the local conformation of the protein. The integration of the equations of motion for
this quantum-classical system is achieved with the four following steps. First, for a given
conformation of the protein, that is, for a given set of atomic coordinates, we find the possible
quantum eigenstates of the amide I excitation; secondly, one eigenstate is selected, by using a
Monte Carlo step; thirdly, the influence of the amide I excitation on the classical motion of
hydrogen-bonded atoms is determined; and fourthly, the extended classical equations of
motion for the atoms are integrated which leads to a new set of atomic positions. In 2009, most
of the serial code modification of GROMACS has been finished, although the new code has yet
to be tested and applied. This work was performed by Dr. Holly Freedman in collaboration with
Dr. Paul Martel, of CBME, Univ. Algarve Faro.
92
Concerning objective 4) above, and in collaboration with Dr. Oliva Cantu Ros and Prof. Manuel
G. Velarde, of the Instituto Pluridisciplinar of the Universidad Complutense, Madrid, Spain, the
connections between the solectron model developed by Prof. Velarde for the interaction of
electrons with the vibrations of a nonlinear lattice, with other nonlinear models studied
previously have been investigated. It has been found that the solectron states studied so far
have energies that are much larger than those explored in the previous nonlinear models and
that, at lower energies and in some parameter regimes, the conduction properties of solectrons
are similar to those predicted by other nonlinear models. We have also started to apply these
nonlinear models to electron transfer in order to investigate the influence of the distance
between donors and acceptors on the efficiency of transfer.
Finally, concerning objective 5) above, the provisional patent application nº 104302 K, made to
Portuguese Patent Office (INPI) in 2008, was classified in second place by experts from the
University of Austin who met with LC in 2009. However, the final decision by the Rector of the
University of Algarve was not to turn it into a full patent yet, profiting from the fact that its
subject matter is not yet in the public domain. On the other hand, another provisional patent
application was made in another area (Cruzeiro, L. 2009. Portuguese Provisional Patent
Application nº 104832 N).
8d. Productivity
1. Cruzeiro, L. 2009. The Davydov/Scott model for energy storage and transport in proteins.
Journal of Biological Physics 35: 43-55. IF=0.646.
2. Cruzeiro, L, Lopes, PA. 2009. Are the native states of proteins kinetic traps? Molecular Physics
107: 1485-1493. IF=1.634.
In press
3. Cruzeiro, L. (in press). The VES hypothesis and protein misfolding, discrete and continuous
dynamical systems series S.
proceedings)
1. Cruzeiro, L. (in press). Protein Folding. In: M. Springborg (Ed), Chem. Modelling, Specialist
Periodic Reports of the Royal Society of Chemistry, UK, Royal Society of Chemistry, London.
proceedings)
93
Cruzeiro, L. 2009. Método para deduzir a estrutura tridimensional de uma proteína a partir da
sequência de aminoácidos. Portuguese Provisional Patent Application nº 104832 N.
The Biophysics group has an on-going collaboration with the Nonlinear Physics Group of the
University of Seville, Seville, Spain and in particular with Prof. Francisco Romero Romero (the
leader) and Dr. Jesús Cuevas Maraver, on the general theme of protein folding and function. In
particular we have been studying quantum thermalization schemes based on Lindblad
operators.
L Cruzeiro is also an honorary research fellow at the Mathematics Department of Heriot-Watt
University, Edinburgh, Scotland, UK, where the Biophysics group collaborates with Prof. Chris
Eilbeck on the interaction of quantum particles with mechanical vibrations, with models that are
an extension of those applied to energy transfer in proteins.
The collaboration started in 2008 with Prof. Manuel Garcia Velarde of the Instituto
Pluridisciplinar of the Universidad Complutense de Madrid continues and in 2009 it included
three visits by L Cruzeiro to Prof. Velarde’s Institute (see description of the achievements within
objective 4. in section Main Achievements).
A new collaboration was started in 2009 with Prof. Michael Springborg, of the University of
Saarland, Saarbrücken, Germany, who visited the Biophysics group and CCMAR in the end of
October, 2009. This collaboration is related to the extension the Davydov/Scott model to include
another vehicle for energy transfer in proteins, namely, NH stretch vibrations. Prof. Springborg
is an expert in ab initio quantum methods and his group will evaluate the coupling parameters
needed for the simulation of vibrational energy storage and transfer in proteins in the form of
NH stretch excitations.
94
8e. Future research
1. Objectives
The general aim of the Biophysics Group in the near future is to consolidate and develop further
the lines of research that it has been pursuing in the last few years on protein folding and
function, and to apply for patents whenever appropriate.
One specific objective is to continue the intensive computer simulations to demonstrate that
proteins can have more than one thermodynamically stable structure, i.e., that their free energy
landscape is not funnel shaped, as is usually assumed, but that it is instead multi-funnel shaped.
The up to100 nanosecond simulations made so far support the multi-funnel landscape, but a
more convincing case requires microsecond simulations or longer. This computationally
intensive work will be done together with Dr. Paulo Afonso Lopes and Dr. Pedro Medeiros, of
CITI, UNL, Lisbon and also at the Milipeia supercomputer of LCA, UC, Coimbra. In parallel, a
second specific objective is to develop further the new kinetic mechanism for folding (Cruzeiro,
L. in press. Protein Folding, Specialist Periodic Reports of the Royal Society of Chemistry)
A third specific objective is to use the code modifications of GROMACS described in the section
Main Achievements to understand how mechanical work, such as protein structural
rearrangements, can be influenced by amide I excitations. Dr. Holly Freedman has been thinking
about how this might happen, and has some insights based on her previous work, which she
continues to pursue, on microtubule dynamics. Specifically, she has been running simulations of
several systems consisting of two tubulin protofilaments that are a subset of the ring of
protofilaments that makeup the microtubule. One striking observation from the trajectories is
the variability in the structure of one of the helices in the vicinity of the site of GTP hydrolysis. A
similar folded/unfolded double-state system has also been reported to be associated with the
hydrolysis of ATP in actin. Thus, after the new code has been fully tested, Dr Holly Freedman will
investigate the possibility that the propagation of an amide I excitation can lead to the
secondary-structural transitions observed in actin and tubulin, using the GROMACS
modifications that she has been implementing. This work is in collaboration with Dr. Paulo
Martel, of CBME, Univ Algarve, Faro.
A fourth specific objective is the application of the VES hypothesis, that is, the possibility that
vibrational excited states drive protein conformational changes, and thus, that they drive
protein function, to misfolding diseases. The project Project “Transporte mediado por solitones
en sistemas anarmonicos mono- y bi-dimensionales'' (ref. FIS2009-06585-E/FIS), in collaboration
with Dr. Patrícia Faísca of CFMC, UL, Lisbon, where other, more classical approaches to
misfolding diseases are also pursued, is related to this theme.
A fifth specific aim is to determine operators (Lindblad operators) capable of establishing the
coupling of the amide I excitation to a thermal bath, within a full quantum mechanical
framework. This work is in collaboration with the Nonlinear Physics Group of the University of
Seville and with Prof. Chris Eilbeck, as well as with Prof. Ana Nunes, of CFMC, University of
Lisbon.
A sixth specific aim is to continue the collaboration with the group of Prof. Manuel G. Velarde, of
the Instituto Pluridisciplinar of the Universidad Complutense, Madrid, Spain, exploring the
95
solectron concept proposed by Prof. Velarde as another mechanism for electron conduction in
condensed matter and to extend these studies to electron transfer in proteins.

Current funding
FCT Pluriannual funding.
FIS2009-06585-E/FIS, Transporte mediado por solitones en sistemas anarmonicos mono- y bidimensionales, Modalida: EF- EXPLORA. Total funding: 40.000 EUR, from Ministerio de Ciencia e
Innovacion, Spain. Coordinator: Manuel Garcia Velarde, Universidade Complutense de Madrid.
10.000 CPU hours, Estabilidade dinâmica do prião humano, in the supercomputer Milipeia of the
Laboratory for Advanced Computing of the University of Coimbra. PI: L Cruzeiro.
ca 23.340 EUR per annum, Postdoctoral grant for Dr. Holly Freedman, funded by FCT, ref.
SFRH/BPD/41143/2007. Supervisor: L Cruzeiro.
92.260 EUR, FCT PTDC/SAU-GMG/098274/2008, Intermediates states: At the crossroads of
pathways to folding and pathways to disease, 2010-2013. PI: Dr. Patrícia Faísca, CFMC,
University of Lisbon. Task leader: L Cruzeiro.

Pending funding (under re-evaluation)
198.003 EUR, FCT PTDC/BIA-PRO/098335/2008, Do protein native structures correspond to the
global minima of the free energy?, 2010-2013. PI: L Cruzeiro, in collaboration with Dr. Paulo
Afonso Lopes and Dr. Pedro Medeiros, of CITI, UNL, Lisbon.
96
Cellular and Inorganic Biochemistry (CIB)
BioVanadium, now consolidated as Cellular and Inorganic Biochemistry (CIB)
Manuel Aureliano Pereira Martins Alves
3. Location of group (Host institution)
Main source is FCT Pluriannual funding, about 4.000 EUR/year. The funds are apply to reagents,
small equipment for the Lab, conferences registration and travel wherever adequate or other
expenses needed for normal development of the research pursue by the group.
1. Objectives
Research topics in 2009:
-
Decavanadate effects on biology
-
Vanadate compounds effects in glucose uptake in adipocytes
-
Oxidative stress in neurons
The contribution of decavanadate to biology was highlighted in two papers (Aureliano, M. 2009;
Aureliano, M, Crans, DC. 2009) and also the contribution of vanadate to bone mineralization was
reviewed (Laizé, V et al. 2009). A new area research topic was started by the publishing of the
effects of vanadium on glucose uptake in rat adipocytes (Pereira MJ et al. 2009). In the order
hand, the research on the effects of vanadium in muscle proteins was still maintained and a
contribution about the interaction of decavanadate with actin was published (Ramos, S et al.
2009). A short paper was also published about the oxidative effects of peroxynitrite in neurons
suggesting that actin filaments disruption is an early event of stress (Tiago, T et al. 2009).
97
8d. Productivity
1. Aureliano, M. 2009. Decavanadate: a journey in a search of a role. Dalton Transactions 42:
9093-9100. IF=4.081.
2. Aureliano, M, Crans, DC. 2009. Decavanadate (V10O286-) and oxovanadates: Oxometalates
with many biological activities. Journal of Inorganic Biochemistry 103(4): 536-546. IF= 3.252.
3. Laize, V, Tiago, DM, Aureliano M, Cancela, ML. 2009. New insights into mineralogenic effects
of vanadate. Cellular and Molecular Life Sciences 66(24): 3831-3836. IF= 6.090.
4. Pereira, MJ, Carvalho, E, Eriksson, JW, Crans, DC, Aureliano, M. 2009. Effects of decavanadate
and insulin enhancing vanadium compounds on glucose uptake in isolated rat adipocytes.
Journal of Inorganic Biochemistry 103(12): 1687-1692. IF=3.252.
5. Ramos, S, Duarte, RO, Moura, JJG, Aureliano, M. 2009. Decavanadate interactions with actin:
cysteine oxidation and vanadyl formation. Dalton Transactions 38: 7985-7994. IF=4.081.
6. Tiago, T, da Silva, DM, Samhan-Arias, AK, et al. 2009. Actin cytoskeleton disruption in na early
event upon exposure to cerebellar granule neurons to SIN-1-induced oxidative stress. Free
Radical Research 43: 70-70. IF= 2.215.
proceedings)
proceedings)
98
8e. Future research
1. Objectives
Study the mode of interaction between decavanadate and vanadyl with actin. Analyze the
structural and functional effects of oxometalates on calcium pump ATPase. Rationalize the
contribution of peroxynitrite to neuron cytoskeleton damage. Analyze the mechanism of
immunosupressors mediation on diabetes.

Current funding
The same fund as 2009, plus eventual projects to be submitted and approved by FCT.
99
Cellular, Molecular and Analytical Studies (LECEMA)
Cellular and Molecular Studies (LECEM), now consolidated as Cellular, Molecular and
Analytical Studies (LECEMA)
Eduardo Jorge Sousa Rocha
Total of funds spent in 2009:
35.750,00 EUR, FCT Pluriannual funding.
48.311,17 EUR,FCT PTDC/MAR/68885/2006, Lipid metabolism disruption in marine fish by
xenobiotic ligands of peroxisome proliferator-activated receptors (PPARs).
13.857,06 EUR, FCT PTDC/MAR/70436/2006, An integrative study on the toxicopathic lesions in
Portuguese estuarine fishes - Assessing injury impact and toxicogenomic implications in
experimental models.
11.082,41 EUR, FCT PTDC/MAR/68106/2006, The modulation of retinoic acid signalling
pathways by environmental pollutants in teleosts.
1. Objectives
The general strategic scientific objectives for the initial small group set up back in 2003 were to
build a multidisciplinary group and to establish a well-equipped lab space at the CIIMAR new
facilities in Porto. We aimed to build a team able to support multidisciplinary studies in field and
laboratory settings, and well positioned to establish cooperation with other national and
international groups. The research efforts at the LECEMA (formerly LECEM) soon targeted both
fundamental and applied studies, correlating structure with function in selected fish and
molluscs, either in normal and pathological scenarios (namely in toxicological contexts). Some
emphasis was and is being put on influences of steroid hormones and xenobiotics in unexplored
targets, from molecular (eg. mechanisms of imposex and of PPARs involvement in peroxisomal
100
changes) to organ levels (eg. liver responses to subacute toxicity). More recently we wanted to
expand our expertise to chemical analysis in parallel to biochemical approaches, aiming data
integration. As a dynamic group, we established a strategy to expand our studies to in vitro
approaches, namely for getting mechanistic insights on some phenomena we discovered in vivo.
We will increasingly use in vitro systems to study fundamental and toxicological problems.
As to non-experimental studies in aquatic biology, our main objective has been to contribute for
a better knowledge of the structure and function of the fish liver, and other related aspects of
the digestive tract, considering gender and seasonal effects. More recently, those studies also
involved the kidney, establishing a comparative approach between events detected in liver and
that are for example explored as a new thesis of endocrine (estrogenic) modulation of
peroxisomal function. Comparative studies with phylogenetic emphasis were also made in the
digestive apparatus of molluscs, including hydrothermal-vent bivalves from the Azores. These
approaches aimed to provide sound baseline data to support field and experimental (inc.
toxicopathological) studies, which were initiated combining biomarkers with analytical
chemistry targeting toxicants.
As to experimental studies, our aim has been to target biological problems related with
endocrine regulation vs disruption in aquatic animals, namely on liver and reproductive system.
Examples of those are: 1) how and to what extent fish peroxisomes are regulated and disrupted
by estrogenic compounds and xenobiotic ligands of PPARs; and 2) what are the fine molecular
mechanisms involved in the development of male penis and female imposex. In additional
experiments, in cooperation with other research groups at CIMAR and from other institutions,
our group wanted to intervene implementing histopathology for diagnosis of toxicophatic
lesions and also testing new quantitative approaches at tissue level for evaluating the intensity
of effects of reference xenobiotics, like pesticides.
We also set the aim to put our expertise in some ambitious field studies in toxicology, in
cooperation with other groups, with a focus on endocrine disruption of biological targets and
their correlation with a wide range of contaminants, for which we validated methods. We aim to
cover a wider range of relevant contaminants, particularly those with known endocrine /
neuroendocrine disruption effects, including natural and man made chemicals, such as
pharmaceuticals. Under a current project, we are conducting surveys in several main estuaries
in Portugal. In the near future, with already established partners, we aim to offer integrated
databases and innovative approaches such as the one inserted in a very recently funded project
for tackling endocrine disruption by studying an invertebrate repertoire of nuclear receptors.
Besides research, we want to continue to give support to post-graduate studies. Such aim is
currently being fulfilled by hosting master and doctoral students, and also by supporting courses
of the Master in Marine Sciences – Marine Resources (U. Porto). We are also involved in the
development of the PhD Program of Marine and Environmental Sciences (U. Porto, U. Aveiro).
Highlights of main research achievements
Line 1) Peroxisomes and related nuclear receptors (PPARs, RXRs, RARs) - Interplay mechanisms,
endocrine modulation, and metabolic consequences of disruption
101
-
In 2009 we proved our hypothesis that PPARs gene expression varies along the fish
breeding cycle. Moreover, we established that PPARα expression in females followed
the same annual variation pattern as peroxisome volumes and enzyme activities, and an
inverse pattern relatively to the salmonid type of annual plasma estradiol levels. The
data agreed with the idea that PPARα is under estradiol modulation and that cross-talk
between this receptor and the estrogen receptor possibly exists. This has implications
on biology and toxicology. In the same vein, we also reported the first sequence of the
peroxisomal 17β-hydroxysteroid dehydrogenase 4 in fish. In agreement to our previous
data and hypothesis, the seasonal expression kinetics in females was negatively related
to key reproductive physiology events, and it was in accordance with a putative dual
role for this enzyme in the liver: fatty acid and estradiol catabolism throughout the
annual breeding cycle of female trout. This has to be tackled with in vivo experimental
assays. Other new data was generated in trout and other models to be later published.
We also started to learn that our sex-steroid modeling theory of peroxisomes seems to
be organ-specific, as our first insight on kidney show a different seasonal pattern than in
liver.
Line 2) Toxicopathology, Toxicogenomics and Chemical Toxicology
-
We published the first major chemical survey for xenoestrogenic endocrine disrupting
compounds (EDCs) in Portugal, covering three estuaries: Sado, Mondego and Douro. The
approach integrated seasonal and spatial distribution. We covered EDCs either of animal
(estradiol and estrone), vegetal (daidzein, genistein and biochanin A), pharmaceutical
(17a-ethynylestradiol) or industrial (bisphenol A, 4-octylphenol, 4-nonylphenol) origins.
Several compounds existed in high amounts in water, and taking in account that these
pollutants may additively contribute to endocrine disruption, we concluded that all
estuaries had a biologically significant pollution by EDCs. In the Douro, almost all EDCs
existed in all seasons and, therefore, might have been contributing to endocrine
disruption, as revealed by the high rate of ovotestis in local fish. Also in Douro, we
started to tackle pharmaceuticals, by validating methodology. With molecular tools, we
made advances on androgenic and anti-androgenic influences in fish gonads, e.g., by
proving that some androgens can modulate / promote (or disrupt) early oocyte growth.
Other studies) Lines 1 and 2 Related Contributions to Aquatic Biology
-
When studying the liver both for peroxisome biology and for toxicology, we continued
to generate sound reference data that establishes ranges of normality for our model
species, by using citology, histochemistry, and stereological approaches on both fish
liver and kidney, and organs of mollusks.
Duplication of the publications output
-
102
In the wake of the moderate but steady increase in the scientific output of previous
years, we published 25 international publications in 2009 (22 articles and 3
proceedings), thus more than doubling the 2008 output. So, we had a mean production
rate of about 2.2 peer-reviewed publications / per PhD member / per year. However,
the real number is an average of 4.2 articles / per % PhD / year (facing the total % of
engagement of the PhD members in CIIMAR). Additionally, the team attended
international meetings in biology, physiology and toxicology, making about 20
presentations (posters and platform talks).
Reinforcing scientific networking
-
From our 25 international publications, we had 7 papers in with colleagues (at least one
co-author) from abroad, so making 31% of our scientific output with international
networking. Also, 55% of our scientific output had at least 1 author from other national
group. All these figures materialized our aim to consistently build collaborations.
Support to advance training
-
We concluded 3 undergraduate projects. Additionally, we continued to host 6 PhD
students. Three master students entered. Two young research grants were attributed.
We offered a course in cell and molecular biology (Master in Marine Sciences - U.Porto).
International recognition by publishers
-
Team members served as peer-reviewers in indexed journals. The PI continued as
Editor-in-Chief of Comparative Hepatology (BioMed Central - Springer). The PI was
invited and the group submitted a book chapter on fish ovary development and
function, to be included in the series Hormones and Reproduction in Vertebrates
(Academic Press).
8d. Productivity
1. Batista-Pinto, C, Rocha, E, Castro, LFC, Lobo-da-Cunha, A. 2009. Seasonal and gender variation
of peroxisome proliferator activated receptors expression in brown trout liver. General and
Comparative Endocrinology 161: 146-152. IF=2.732.
2. Carrola, J, Fontaínhas-Fernandes, A, Matos, P, Rocha, E. 2009. Liver histopathology in brown
trout (Salmo trutta f. fario) from the Tinhela River, subjected to mine drainage from the
abandoned Jales mine (Portugal). Bulletin of Environmental Contamination and Toxicology 83:
35-41. IF=0.992.
3. Castro, LFC, Rocha, MJ, Lobo-da-Cunha, A, Batista-Pinto, C, Machado, A, Rocha, E. 2009. The
17β-hydroxysteroid dehydrogenase 4: gender and seasonal gene expression in the liver of
brown trout (Salmo trutta f. fario). Comparative Biochemistry and Physiology B - Biochemistry
Molecular Biology 153: 157-164. IF=1.607.
Comparative Biochemistry and Physiology C - Toxicology & Pharmacology 149: 58-64. IF=2.582.
103
5. Jordanova, M, Miteva, N, Santos, N, Malhão, F, Rocha, E. 2009. Crystalline inclusions in the
liver of wild female Ohrid trout (Salmo letnica Kar.). Tissue & Cell 41: 281-285. IF=1.011.
(Bivalvia: Mytilidae). Marine Biology 156: 959-967. IF=1.999.
7. Kortner, TM, Rocha, E, Arukwe, A. 2009. Androgenic modulation of early growth of Atlantic
cod (Gadus morhua L.) previtellogenic oocytes and zona radiata-related genes. Journal of
Toxicology and Environmental Health, Part A 72: 184-195. IF=1.724.
8. Kortner, TM, Rocha, E, Arukwe, A. 2009. Previtellogenic oocyte growth and transcriptional
changes of steroidogenic enzyme genes in immature female Atlantic cod (Gadus morhua L.)
after exposure to the androgens 11-ketotestosterone and testosterone. Comparative
Biochemistry and Physiology A - Molecular & Integrative Physiology 152: 304-313. IF=2.196.
9. Lobo-da-Cunha, A, Ferreira, I, Coelho, R, Calado, G. 2009. Light and electron microscopy study
of the salivary glands of the carnivorous opisthobranch Philinopsis depicta (Mollusca,
Gastropoda). Tissue & Cell 41: 367-375. IF=1.011.
10. Madureira, T, Barreto, J, Rocha, MJ, Cass, Q, Tiritan, ME. 2009. Pharmaceutical trace analysis
in aqueous environmental matrices by liquid chromatography-ion trap mass spectrometry.
Journal of Chromatography A 1216: 7033-7042. IF=4.101.
11. Marcos, R, Malhão, F, Monteiro, R, Rocha, E. 2009. Gender and aging in the liver: preliminary
data using design-based stereological methods. Microscopy and Microanalysis 15(S3): 45-46. IF=
3.035.
12. Marcos, R, Santos, M, Santos, N, Malhão, F, Ferreira, F, Monteiro, RAF, Rocha, E. 2009. Use
of destained cytology slides for the application of routine special stains. Veterinary Clinical
Pathology 38: 94-102. IF=0.793.
13. Monteiro, SM, Rocha, E, Mancera, JM, Fontaínhas-Fernandes, A, Sousa, M. 2009. A
stereological study of copper toxicity in gills of Oreochromis niloticus. Ecotoxicology and
Environmental Safety 72: 213-23. IF=2.133.
14. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Franquinho, F, Monteiro, RAF, Rocha, E. 2009.
Histological and stereological characterization of brown trout (Salmo trutta) trunk kidney.
Microscopy and Microanalysis 15(S3): 1-2. IF= 3.035.
15. Ribeiro, C, Pardal, MA, Martinho, F, Margalho, R, Tiritan, ME, Rocha, E, Rocha, MJ. 2009.
Distribution of endocrine disruptors in the Mondego River estuary (Portugal). Environmental
Monitoring and Assessment 149: 183-193. IF=1.356.
16. Ribeiro, C, Pardal, MA, Tiritan, ME, Rocha, E, Rocha, MJ. 2009. Spatial distribution and
quantification of endocrine disrupting chemicals in Sado River estuary by solid phase extraction
and high-performance liquid chromatography with diode-array detection. Environmental
104
17. Ribeiro, C, Tiritan, ME, Rocha, E, Rocha, MJ. 2009. Seasonal and spatial distribution of several
endocrine disrupting compounds in the Douro River Estuary, Portugal. Archives of
Environmental Contamination and Toxicology 56: 1-11. IF=1.743.
18. Rocha, E, Rocha, MJ, Galante, MH, Silva, MW, Monteiro, RAF. 2009. The hepatocytes of the
brown trout (Salmo trutta f. fario): A stereological study of their number and size during the
breeding cycle. Ichthyological Research 56: 43-54. IF=0.635.
19. Santos, M, Marcos, R, Santos, N, Malhão, F, Monteiro, RAF, Rocha, E. 2009. A stereological
study on the sub-populations of rat liver macrophages and on their numerical relation with the
hepatocytes and stellate cells. Journal of Anatomy 214: 744-551. IF=2.134.
20. Santos, MM, Enes, P, Reis-Henriques, MA, Kuballa, J, Castro, LF, Vieira, MN. 2009. Organotin
levels in seafood from Portuguese markets and the risk for consumers. Chemosphere 75: 661666. IF=3.253.
fibres in juveniles of blackspot seabream (Pagellus bogaraveo, Brunnich). Journal of Muscle
Research and Cell Motility 30: 29-39. IF=1.657.
22. Silva, P, Valente, LMP, Malhão, F, Galante, MH, Monteiro, RAF, Rocha, E. 2009. Fibre types in
the skeletal muscle of blackspot seabream (Pagellus bagaraveo Brunnich) juveniles: a
histochemical immunohistochemical and ultrastructural study. Microscopy and Microanalysis
15(S3): 43-44. IF= 3.035.
and hypertrophic growth of lateral muscle in the fish blackspot seabream, Pagellus bogaraveo,
from hatching to juvenile. Journal of Fish Biology 74: 37-53. IF=1.226.
24. Soares, J, Coimbra, AM, Reis-Henriques, MA, Monteiro, NM, Vieira, MN, Oliveira, JM,
Guedes-Dias, P, Fontaínhas-Fernandes, A, Parra, SS, Carvalho, AP, Castro, LF, Santos, MM. 2009.
25. Urbatzka, R, Waterman, B, Lutz, I, Kloas, W. 2009. Exposure of Xenopus laevis tadpoles to
finasteride, an inhibitor of 5-alpha reductase activity, impairs spermatogenesis and alters
hypophyseal feedback mechanisms. Journal of Molecular Endocrinology 43: 209-219. IF=3.221.
proceedings)
proceedings)
105
PhD THESES
Rocha, Co-supervisor: L Valente. (Submitted in 2009, defense in January 2010).
-
2009, Porto, Portugal. LFC Castro – Member of the Organising Committee.
-
In Charge of the Organization of the Regular Seminars of the CIIMAR (LFC Castro,
organized over 25 seminars in 2008-2009).
Collaborative projects continued in 2009, with research groups in Macedonia (Faculty of Natural
Sciences and Mathematics, Univ. Sts. Cyril & Methodius), Japan (several partners), Norway
(NTNU, Trondheim), UK (King’s Colleague and CEFAS), USA (Duke University), Germany (LeibnizInstitute of Freshwater Ecology and Inland Fisheries, Berlin, Germany) and Thailand (Kasetsart
University and adding King Mongkut’s University of Technology, Bangkok). Examples of
collaborative papers are:
-
Jordanova, M, Miteva, N, Santos, N, Malhão, F, Rocha, E. 2009. Crystalline inclusions in
the liver of wild female Ohrid trout (Salmo letnica Kar.). Tissue & Cell 41: 281-285.
-
Kortner, TM, Rocha, E, Arukwe, A. 2009. Androgenic modulation of early growth of
Atlantic cod (Gadus morhua L.) previtellogenic oocytes and zona radiata-related genes.
Journal of Toxicology and Environmental Health, Part A 72: 184-195.
-
Kortner, TM, Rocha, E, Arukwe, A. 2009. Previtellogenic oocyte growth and
transcriptional changes of steroidogenic enzyme genes in immature female Atlantic cod
(Gadus morhua L.) after exposure to the androgens 11-ketotestosterone and
testosterone. Comparative Biochemistry and Physiology A - Molecular & Integrative
Physiology 152: 304-313.
106
-
Urbatzka, R, Waterman, B, Lutz, I, Kloas, W. 2009. Exposure of Xenopus laevis tadpoles
to finasteride, an inhibitor of 5-alpha reductase activity, impairs spermatogenesis and
alters hypophyseal feedback mechanisms. Journal of Molecular Endocrinology 43: 209219.
The PI was formerly invited to integrate his team in a European network project named
Biological Effects Quality Assurance in Monitoring Programmes (BEQUALM). Contacts were
made so that this can happen in the future, and there is one ongoing joint study with the CEFAS
(UK) on fish carcinogenesis. The PI continued to be a member of the executive commission of
the recent inter-university PhD Program in Marine and Environmental Sciences (Univ. Porto &
Univ. Aveiro), and cooperated in 2009 in the eventual launching of a new Doctoral Program with
the University of Galicia (Spain), upon request of the Porto and Galicia authorities and the Univ.
Porto Rector. The PI’s Group provided advanced training and classes in those Master and PhD
programs.
Still in 2009, our group hosted for 6 months a post-doc fellow from NTNU (Norway).
Other than running FCT funded projects, no further contract research was developed.
LECEMA participated in the meetings promoted by the North Regional Coordination and
sustainable development of marine research in the North of Portugal and Galicia region that
resulted in a final report “Regional Agenda for the Sea”, published in February 2009.
8e. Future research
1. Objectives
The overall strategic goals defined by the PI for a period of 3 years were established in the end
of 2008, and targeted: 1) increase in 10% the papers with international partners and the articles
average impact; 2) increase in 10% the number of papers/PhD/year; 4) to conclude 6 PhD
theses; 5) focus on basically 2 research lines (see below), not discarding interesting / related
contributions to the aquatic biology field; and 6) run an annual course on cell and molecular
biology techniques (jointly with a course in a Master’s). As all research is a dynamic process,
tuning is now made and it may be further inserted over time and depending on funding. Note
also that the Lines described below actually integrate with each other when it concerns the
toxicological aspects.
Line 1) Fish peroxisomes and related nuclear receptors (PPARs, RXRs, RARs); interplay
mechanisms, endocrine modulation, and metabolic consequences of disruption
-
We will continue to publish data derived from past and current efforts, proving that fish
peroxisomes are modulated by sex steroids, particularly in liver and by estrogenic
107
stimuli, apparently involving PPARs. Also, we will study (both in vivo and in vitro)
interactions between peroxisomal / PPARs activating vs repressing (estrogens) ligands.
Work is already being conducted with the so-called peroxisome proliferators (PPs).
Some of them have been detected worldwide, and concern about their use is growing
due to their persistence, toxicity and bioaccumulation. We are exploring the hypothesis
that those exogenous PPARs ligands may disrupt lipid metabolism in fish, affecting
health, survival, growth and, lipid/FA profiles. A gene array approach is integrated. We
aim contributing with new insights to understand lipid and energy metabolism control in
fish, with possible application on risk assessment and aquaculture. In parallel, it will be
also described the action of PPs on other genes not involved in lipid metabolism and
possibly not under PPARs control, as the result of the use of advanced molecular tools. It
is known that PPARs cross-talk with RXRs, and in this vein we developing a project that
involves a multiparametric approach, combining the study of variations at the gene and
protein (enzymes) levels of key disruption prone targets, but also changes in sexual
development and reproduction. We expect to identify relevant metabolic pathways
prone to disruption by RARs, RXRs, PPARs agonists. In the future, we aim an overall
better understanding of the molecular mechanisms involved in peroxisome regulation
(ER and PPARs cross-talk); such insights have a both biomedical and aquatic toxicology
interest.
-
Nuclear receptors (NRs) form an important superfamily of ligand-dependent and
independent transcription factors that regulate many biological processes. Extending
our efforts in fish peroxisome-related NRs, we also expect to show that the
understanding of invertebrate NR biology is essential to develop high-quality integrating
models and characterizations of risk from chemical exposure. Our current purpose is to
develop a methodology which rapidly screens chemical compounds for their ability to
interact with invertebrate NRs and thus cause physiological perturbation. Given that we
are using a broad phylum sampling, the data can be interpreted in ecosystem scale.
Such data should significantly impact the comprehension of endocrine system evolution
in the Bilateria.
Line 2) Toxicopathology, Toxicogenomics and Chemical Toxicology
-
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We launched two years ago a multidisciplinary project, integrating from chemical
toxicology to transcriptomics, which will set grounds for an expansion and cooperative
efforts into future proteomics and metabolomics. The project background is the fact
that toxicophatic injuries in estuarine fish are increasing. Histopathology often reveals
liver lesions and intersex condition. These situations translate either acute and/or
chronic chemical stress. Field surveys targeting fish toxicopathic lesions are being made
in Europe and USA, in parallel with the use of other biomarkers and chemical analyses.
Pathology surveys are very scarce in Portugal and so we aim to be the national leading
group on this context. Our current project (that we want to integrate in the future with
parallel bioaccumulation approaches) is unique in making a 1st systematic survey of
toxicopathic lesions in Portuguese estuarine fish, whereas experimentally tackling
effects of lesions at diverse biological levels and correlations with water contamination.
Some of the near future aims are to: 1) document toxicopathic fish lesions in estuaries
in the north and centre; 2) clarify in what extent the lesion type and severity relates
with water chemical pollution, namely by endocrine disruptors; 3) to expand our range
of validated methods for chemical screening and apply them from North to South, in
cooperation with ecology groups; 4) know better fish carcinogenesis and find ways to
early detect related lesions; 5) clarify if/in what extent hepatic necrosis and neoplasia
may interfere with general liver function and detoxification, growth/survival; 6)
conclude about gender susceptibility to toxicopathic lesions and implications of fish
neoplasia; 7) study gene signatures of tumour liver progression in a fish model; 8) to use
focused cDNA array tec to support experimental modeling; In proposed projects, we
target not only liver and gonads toxicity by endocrine mimics and by some
pharmaceuticals, but included related hypothesis driven incursions into the
neuroendocrine system. Expect results will contribute to mechanistic insights of
endocrine disruption of the brain-gonad-liver axis. Also, in the wake of our current
works with grey mullets, one proposed idea is to look at impacts of pollution load on
mullet populations in North Portugal, by focussing on genetic diversity and on pollutioninduced selection of allelic variants. SNP analyses would allow a comparison of the
altered phenotype (lesions) with the presence of allelic variants of target genes
suggested to be involved in the formation of adverse effects. This would be an effort to
link the phenotypic changes to the noted diplotypes of the animals.
Other studies) Lines 1 and 2 Related Contributions to Aquatic Biology
-
When studying the above issues, we will continue to generate sound reference data that
establishes ranges of structural and functional normality for our model species, mainly
fish and mollusks. In this regard, bivalves will be incorporated, namely in Line 2 works.
Other issues) Internationalization and Increase of Scientific Relevance
-
We aim to continue the sustained increase in the internationalization of our laboratory
and scientific output, namely by venturing on joint projects and networks with selected
partners, especially at European level but also cooperating with associates in Asia and
USA. With such partnerships and multidisciplinary studies we want to increase the
relevance of our contributions to the aquatic cell / molecular biology and toxicology.

Current funding
184.929 EUR, FCT PTDC/MAR/68885/2006, Lipid metabolism disruption in marine fish by
xenobiotic ligands of peroxisome proliferator-activated receptors (PPARs). 07/2008-06/2011.
153.168 EUR, FCT PTDC/MAR/70436/2006, An integrative study on the toxicopathic lesions in
Portuguese estuarine fishes – Assessing injury impact and toxicogenomic implications in
experimental models. Total funding 187.028 EUR, in cooperation with UTAD and Univ Coimbra.
10/2008-09/2011.
71.164 EUR, FCT PTDC/MAR/68106/2006, The modulation of retinoic acid signalling pathways by
environmental pollutants in teleosts. Sept 2008-August 2011. Total funding: 188.980,00 EUR, in
cooperation with the LEGE and LETOX Groups of CIMAR.
109
64.928 EUR, FCT PTDC/MAR/105199/2008, The invertebrate repertoire of nuclear receptors:
evolutionary and endocrine disruption insights. Total funding: 129.856,00 EUR, in cooperation
with LETOX group of CIMAR.

180.000 EUR, FCT PTDC/AAC-AMB/104970/2008, Endocrine disruption by androgen receptor
antagonists - First chemical survey in a Portuguese River and new insights on their impact on the
reproductive biology of fish.
190.704 EUR, FCT PTDC/CVT/115618/2009, Peroxisomes, estrogens, and nuclear receptors:
cross-talk evaluation in hepatocytes of the trout as a fish model organism - impacts on lipid
metabolism.
145.000 EUR, FCT PTDC/MAR/112115/2009, Neuroendocrine disruption in fish induced by
human psychopharmaceuticals and potential impact of salinity.
142.000 EUR, FCT PTDC/AAC-AMB/115162/2009, Impacts of anthropogenic pollution load on
grey mullet populations (Mugil cephalus) in North Portugal - Focussing on genetic diversity and
on pollution-induced selection of allelic variants.
120.000 EUR, FCT, The effects of E2 and related endocrine disruptors on neural cells: Lessons
from zebrafish.
110
Maria Teresa Sa Dias de Vasconcelos
In the year 2009 the CS&B Lab was involved in two financed projects/protocols at the National
level and one at the International level, both as a coordinator (Portuguese part). National
projects/protocols was financed by two private companies (Aquaculture Company, Petróleos de
Portugal S.A.-Refinaria do Porto) whereas the international project was financed by FCT and by
the Spanish partner (project ERA-AMPERA/0003/2007). Another source of financing was the
Pluriannual FCT funds attributed to PhD researchers and doctoral fees. Total funds involved in
the mentioned period were ca. 50.000 EUR. A part of those funds (ca. 46%) were obtained
through contract-research activities (in cooperation with private companies), whereas the
research project financed by FCT and project partner accounted for ca. 11% and “Pluriannual
FCT funds” and doctoral fees represented ca. 43% of the total funds.
1. Objectives
Research carried out at CS&B Lab intends to give a contribution for the comprehensiveness of
biogeochemical processes in aquatic environment and soil in order to be able to find
remediation solutions when required. It may be also relevant for risk assessment purposes.
Main objectives for 2009 were as follows:
-
To proceed the studies on mutual interactions among phytoplankton (mainly
cyanobacteria) and emerging contaminants (endocrine disrupters and pharmaceutical
compounds);
-
To proceed the studies on the suitability of vascular plants for application in
technologies of remediation, giving emphasis on the plant role in conditioning the
nearby environment. The interactions between plants and the microbial community in
111
its rhizosphere were also accounted for. Included in this topic was the recently launched
investigation on suitable biological remediation processes for the reduction/elimination
of residues of very much used pharmaceuticals compounds which are released by waste
water treatment plants (WWTP) into the aquatic environment;
-
To proceed investigation on bioremediation of beaches contaminated with petroleum
hydrocarbons;
-
To proceed the research on effects of inorganic and organic pollutants, namely metals
and PAHs, on marine organisms.
These studies included financed projects in progress, collaboration protocols, Post Doc, PhD and
MSc projects.
Studies on mutual interactions of phytoplankton and compounds with endocrine disrupting
effects and pharmaceutics are in progress, anchored by a Post Doc and a recently started undergraduation project. One paper was published, one is submitted and another is in preparation. A
Post Doc project on cyanobacteria toxins, carried out in collaboration with CIIMAR LEGE Lab,
also fits this research line. The line focused on mutual influence of metallic species and
cyanobacteria resulted on one paper submitted. Collaboration with LEGE in a PhD project on
freshwater cyanobacteria allelopathic properties resulted in one published paper.
Studies regarding optimization of analytical methods for determination of pharmaceuticals were
carried out under a PhD and a Post Doc projects and two papers are in preparation.
During 2009, a PhD was initiated on the theme: “the barnacle as bioindicator of metals and
polycyclic aromatic hydrocarbons contamination in the Portuguese coast”. The main objectives
of this research are to optimize the analytical methods to measure metals and organic
pollutants in biological marine matrices using the experience of the laboratory; to find out
whether the barnacles in the northern Portuguese coast can be used as bioindicators of these
chemical compounds and in the marine environment; what levels of metals and organic
compounds can be accumulated in these organisms and if those levels influence barnacles
distribution on the coast. Water quality analysis of aquaculture enterprises is also being carried
out and knowledge and experience in quality assurance and control is being achieved.
Studies on the role of vascular plants in conditioning the nearby environment and plant
capability for rhizoremediation (direct plant action combined with microbial role) were
intensified in the ambit of PhD project, which is carried out in collaboration with CIIMAR
Hydrobiology Lab. Still in this line, final results (including analytical methodologies optimization)
of a research project that ended in 2008 were organized for publication. Four articles and a book
chapter were published, two papers were accepted and two submitted/in preparation.
Optimization of methodologies for detection and quantification of compounds released by
vascular plants into the medium (exudates) were carried out with success, in the ambit of a PhD
project and a paper with the results is in preparation.
The investigation of suitable of biological processes for remediation of a soil contaminated with
petroleum hydrocarbons (PHC) are in progress (in the ambit of a PhD, a MSc and under
112
graduated projects) main results being obtained on optimization of expeditious methods to
estimate PHC degradation extent and role/evolution of microbial community. One article in a
book of proceedings was published, a paper was accepted for publication and another one is in
preparation. The research, in collaboration with CIIMAR Hydrobiology Lab (an international
interdisciplinary project), concerning PHC bioremediation in contaminated beaches continued,
involving three fellowship students.
Studies on indoor air quality continued with one MSc thesis.
Collaboration with several small and medium companies and centres continued resulting in
projects and training courses for under-graduate and graduate students with two MSc theses
concluded.
Results of the different studies were presented as oral or poster communications, in eleven
National and International Conferences.
8d. Productivity
Estuarine Coastal and Shelf Science 85: 627-632. IF=1.970.
surfactants on the Cu phytoremediation potential of a salt marsh plant. Chemosphere 75: 135140. IF=3.253.
IF=3.124.
4. Carvalho, PN, Rodrigues, PNR, Basto, MCP, Vasconcelos, MTSD. 2009. Organochlorine
pesticides levels in Portuguese coastal areas. Chemosphere 75: 595-600. IF=3.253.
5. Carvalho, PN, Rodrigues, PNR, Basto, MCP, Vasconcelos, MTSD. 2009. Butyltin levels in several
Portuguese coastal areas. Environmental Monitoring and Assessment 159: 183-190. IF=1.356.
6. Leao, PN, Vasconcelos, MTSD, Vasconcelos, VM. 2009. Allelopathic activity of cyanobacteria
on green microalgae at low cell densities. European Journal of Phycology 44: 347-355. IF=2.270.
salt marsh: a metal clean area?. Environmental Monitoring and Assessment 159: 191-205.
IF=1.356.
113
proceedings)
Books (chapter)
Conference Proceedings
2. Couto, MNF, Vasconcelos, MTSD. 2009. Phytoremediation of hydrocarbons from a refinery’s
soil – the role of rhizosphere. Proceedings of the International Symposium on Contaminated
Soils and Sediments, pp 289-295.
proceedings)
MASTER THESES
Gomes, JMBC. 2009. Chemical characterization and influence of the bottling process of a natural
sparking mineral water. Master thesis, Faculty of Sciences, University of Porto. Supervisor: MC
Basto, Rui Santos (Lab. INETI, S. Mamede de Infesta).
Guedes, JMS. 2009. Study of the indoor air quality in Porto City schools. Master thesis,
Department of Chemistry, Faculty of Sciences, University of Porto. Supervisor: MT Vasconcelos
and MC Basto.
Martins, MLX. 2009. Eco-efficiency: development of assessment tools. Master thesis, Faculty of
Sciences, University of Porto. Supervisor: MC Basto, responsible from Ecoinside: Joaquim
Guedes.
Cooperation with:
114
-
Portuguese Association of Small and Media Companies on Environmental Diagnostics in
the Industry.
-
Real Companhia Velha on Characterization of Port Wine.
-
SMGP - Consultores, Lda on Chemical Characterization of Occupational Environment.
-
Petróleos de Portugal S.A. Refinaria do Porto, GALP Energy Group - Petróleos de
Portugal S.A on Rizoremediação de Hidrocarbonatos de Petróleo em Solos da Refinaria
do Porto.
-
Paracélsia Industria Farmacêutica S.A. on Aluminum Determination on Serum Samples.
-
Hovione FarmaCiencia S.A. on Survey of Some Pharmaceutics Effects on Freshwater
Cyanobacteria. Does Good Manufacturing Practice (GMP) During Pharmaceutics
Manufacture Influence the Biological Response?.
-
Department of Production and Systems, School of Engineering, University of Minho.
-
Small and Media Companies, like Sondar Company and ECOINSIDE - Soluções em
Ecoeficiência e Sustentabilidade, Lda., and Centres, like Centro de Apoio Tecnologia à
Indústria Metalomecânica (CATIM), for training courses and projects for under-graduate
and graduate students, namely for development of MSc theses.
Socrates/Erasmus Bilateral Agreements in Environmental Chemistry (MTSD Vasconcelos)
-
Swiss Federal Institute of Technology, K. Hungerbuehier.
-
SECETOX, Masaryk University, Brno, Czech Republic, Ivan Holoubek.
-
Lab of Physic-Toxic-Chemistry of Natural Systems, Bordeaux University, Bordeaux,
France, Philippe Garrigues.
-
Environmental Analysis Group, National and Kapodistrian Athens University, Greece,
Panayotis Siskos.
-
Biology Faculty, Environmental Sciences and Biochemistry, Barcelona University, Spain,
Anna Riera.
-
Universidad del País Vasco, Bilbau, Spain.
-
Universidad Politecnica de Valencia, Spain.
Cooperation in International Organizations (MTSD Vasconcelos):
-
Member of the Editorial Board of: Environ. Sci. Poll. Res., Springer; Chemistry Central
Journal.
115
-
Portuguese Chemical Society Delegate in "Division for Chemistry and the Environment
(DCE)" of the Federation of the European Chemical Societies, presently European
Chemistry and Molecular Science (EuCheMS).
-
International programs of Quality Control: Lead in Blood. Instituto Nacional de
Seguridad e Higiene en el Trabajo (INSHT), Zaragoza; Metals in Air. INSHT, Barcelona.
Review of scientific papers:
-
Senior CS&B members act very frequently as reviewers for several international
scientific journals.
CS&B participated in the meetings promoted by the North Regional Coordination and
8e. Future research
1. Objectives
CS&B Lab will continue its lines of research in progress, to achieve the final goal of gathering as
much information as possible on mutual interaction between organisms and the medium they
are living in. Several areas previously identified as strategic priorities for consolidation and
growth of the group are already supported by projects in progress, whereas others will be
initiated.
Studies on mutual interactions of phytoplankton and compounds with endocrine disrupting
effects and pharmaceutics will continue under a Post Doc and an under-graduate program in
progress and a new PhD will be started in January 2010. Collaboration with LEGE, from CIIMAR,
will provide the required phytoplanktonic species. A Post Doc project on cyanobacteria toxins,
carried out in collaboration with LEGE will continue.
Determination (including optimization of methods) of residues of pharmaceuticals will go on,
being extended to soils/sediments. Analytical tools are required to search for suitable biological
processes for removal of the emerging contaminants from waters released by WWTPs. A PhD
and a Post Doc programs in progress are focused on this topic. A company devoted to
construction and management of water and WWTP will give the required technical support (a
proposal of a project in cooperation was submitted).
116
Analysis of metals and PAHs in barnacle tissues, as representatives of the sessile marine
biological communities will be performed to assess the bioaccumulation of these chemical
compounds and the use of barnacles as bioindicators of pollution. Relationships between the
distribution of these organisms along the northern coast and the levels of pollutants will be
investigated.
The role of vascular plants in conditioning the nearby environment and capability of such plants
in symbiosis with the microbial community for rhizoremediation of residues of organic
pollutants will continue under a interdisciplinary (mainly Chemistry and Biology) PhD project in
collaboration with CIIMAR Hydrobiology Lab. A new financed research project on this topic will
star at the beginning of 2010, which will also include inorganic (metals) pollutants. The study of
the role of exudates on biogeochemical phenomena at the rhizosphere will be intensified
anchored in both one PhD and one Post Doc program in progress. Optimization of
methodologies for isolation and pre-concentration are crucial for speciation of exudates, which
are present at ultratrace levels in water and soil/sediment matrixes. It is planned to include also
some MSc programs in this topic.
The research to find suitable biological processes for PHC remediation in contaminated soil will
continue in the ambit of a PhD (to be concluded in 2010), an ongoing MSc and an under
graduated project. Related with this, bioremediation of PHC in contaminated beaches
(collaboration with CIIMAR Hydrobiology Lab in the ambit of an international interdisciplinary
project) will be intensified. MSc and under-graduate students are being integrated in this
project.
CS&B Lab will continue to render collaboration with small and medium companies and public
institutions in order to solve environmental problems which require CS&B Lab expertise.
Collaboration with public and private companies will be also useful for training of students, as
their first experience in the work market. In this ambit, new MSc and under-graduate training
programs will be integrated in the next future.

Current funding
26.960 EUR, ERA-AMPERA/0003/2007, OILDEBEACH - Buried oil in the intertidal beach zone:
coupling between beach morphodynamic, natural degradation, forcing mechanisms and
biological activity. Funded by AMPERA Joint Management Committee. CIIMAR responsible:
Almeida CMR. Other participants: Vigo University (Spain), Montpellier University (France), ICBAS
(Portugal). Funding for a one year scholarship and ca. 17.000 EUR from Spanish partner for
hydrocarbon analysis, 2008-2011.
ca. 30.000 EUR, FCT PTDC/MAR/099140/2008, PHYTOBIO, Phytoremediation and
bioremediation of contaminants in salt marshes: plant - microorganisms interactions.
Participants: ICBAS and CIIMAR. Project leader: AP Mucha (Hydrobiology Lab, CIIMAR), CIIMAR
CS&B Lab responsible: CMR Almeida. 2010-2013.
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
PTDC/AAC-AMB/100736/2008, GRINDOOR - Pollutants removal from indoor atmospheres
through phytoremediation by vascular plants. Participants: FEUP/IDMEC (project leader) and
CIIMAR.
REMOPHARVET, Removal of emerging pollutants residues from wastewater treatment plants
effluents. Participants: FCUP, CIIMAR (CS&B - project leader and Hydrobiology Labs) and CQO
Plus company.
FCT PTDC/AAC-AMB/113973/2009, NITROTOX – Interference of Metals and PAHs in Nitrate
Removal Biological Processes: Denitrification vs Anammox Participants: CIIMAR (Hydrobiology project leader and CS&B Labs) and ICBAS.
CyanoNano, Cyanobacteria response to nanomaterials in aquatic ecosystems, Participants:
CIIMAR (LEGE - project leader and CS&B Labs) and FCUP.
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Chemistry and Biological Activity of Marine Natural Products
Chemistry and Biological Activity of Marine Natural Products
Anake Kijjoa
Funds spent in 2009:
-
-
National funding:
148.346,00 EUR, FCT PTDC/MAR/100482/2008 - Bioactive products in marine algae of Azores
(AzoAlg), 2009-2011.
1. Objectives
The objectives of the research group are:
-
Search for bioactive secondary metabolites produced by terrestrial and marine derived
macro- and microorganisms through collaborative bioprospecting programmes;
-
Study of structural features of the isolated compounds by advanced spectroscopic
techniques;
-
Evaluation of biological and pharmacological activities of the crude extracts and pure
compounds obtained from terrestrial and marine derived micro-and microorganisms;
-
Production of bioactive secondary metabolites from marine-derived fungi using the
OSMAC approach;
-
Profiling the secondary metabolites for the chemosystematic evaluation.
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The main achievements of the group are:
-
Isolation and characterization of novel secondary metabolites, some of which possess
new skeleton, from marine sponges collected from the Gulf of Thailand;
-
Delineation of the biosynthetic pathways of the secondary metabolites from the marine
sponge Suberea praetensa;
-
Isolation of pyridoacridine alkaloids from the marine sponge Oceanapia sagittaria and
the discovery of the mechanism responsible for their in vitro antitumour activity;
-
Isolation and identification of novel secondary metabolites with unprecedented
structural features from the cultures of soil and marine fungi.
Scientific output in publications
The group has published so far around 100 peer reviewed papers in the field of bioactive natural
products from terrestrial and marine sources. In 2009, the group has contributed, besides
publications in scientific journals with referees, two chapters for the book with international
circulation.
International scientific networking and recognition
Although maintaining traditional collaborations with the Department of Chemistry and
Biochemistry of the Florida State University (USA), Institute of Marine Science of Burapha
University (Thailand) and Faculty of Agriculture of Kasetsart University (Thailand), the group has
initiated the research collaboration with Prof. Robert Kiss (Laboratory of Toxicology - Institute of
Pharmacy - Free University of Brussels) as well as with Prof. Peter Proksch (Institute of
Pharmaceutical Biology and Biotechnology, University of Düsseldorf, Germany). These
collaborations have resulted in an elaboration of joint projects submitted to FCT (FCT
PTDC/MAR/112708/2009, Characterization and Antitumour Activity Evaluation of Bioactive
Compounds from Marine Derived Fungi) and European Commission (FP7-KBBE-2010-4:
Sustainable culture of marine microorganisms, algae and/or invertebrates for high added value
products-SUSTAMAR).
The number and quality of scientific publications of the principal investigator (Anake Kijjoa) has
been recognized internationally. This is reflected by the participation of A Kijjoa as a member of
Editorial Advisory Board of the scientific journals Marine Drugs and Journal of Natural
Pharmaceuticals. Anake Kijjoa is also a regular reviewer of the manuscripts submitted for
publication in various journals such as Phytochemistry, Phytochemistry Letters,
Ethnopharmacology, Marine Drugs, Food and Chemical Toxicology. The international scientific
community had finally recognized the work of Anake Kijjoa in the field of Marine Natural
Products in 2005 and had chosen him to be a chairman of the 6th European Conference on
Marine Natural Product which took place in Porto, Portugal, 19-23 July 2009.
Besides, A Kijjoa was appointed as a member of the Marine Board Working Group in Marine
Biotechnology of the European Scientific Foundation. As a member of this working group, he
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was invited to coordinate the topics of “Development of Novel Pharmaceutical Products” and
“Biodiscovery/Bioscreening for Novel drugs, compounds and biomaterials” for the Position
Paper of this group which will be published by the Marine Biotechnology Board in 2010.
8d. Productivity
1. Amat, N, Upur, H, Ablimit, A, Matsidik, A, Yusup, A, Kijjoa, A. 2009. Immunomodulatory
effects of abnormal Savda Munsiq, a traditional Uighur medicine, on the combined stress mice.
Journal of Ethnopharmacology 122: 42-47. IF=2.322.
2. Castanheiro, RAP, Pinto MM, Cravo, SMM, Pinto, DCGA, Silva, AMS, Kijjoa, A. 2009. Improved
methodologies for synthesis of prenylated xanthones by microwave irradiation and combination
of heterogeneous catalysis (K10 clay) with microwave irradiation. Tetrahedron 65: 3848-3857.
IF=3.219.
3. Auamcharoen, W, Chandrapatya, A, Kijjoa, A, Silva, Herz, W. 2009. Chemical constituents of
Duabanga grandiflora (Lythraceae). Biochemical Systematics and Ecology 37: 535-537. IF=1.131.
4. Auamcharoen, W, Kijjoa, A, Chandrapatya, A, Pinto, MM, Silva, AMS, Naengchomnong, W,
Herz, W. 2009. A new tetralone from Diospyros cauliflora. Biochemical Systematics and Ecology
37: 690-692. IF=1.131.
proceedings)
1. Kijjoa, A, Vieira, LMM. 2009. Triterpenes from the Plants of the Family Clusiaceae (Guttiferae):
Chemistry and Biological Activities. In: Goutam Brahmachari (Ed). Natural Products: Chemistry,
Biochemistry and Pharmacology, Narosa Publishing House Pvt. Ltd., New Delhi, India, pp. 326381.
2. Cidade, H, Neves, M, Kijjoa, A. 2009. Natural Prenylated Flavones: Chemistry and Biological
Activies- An Overview. In: Goutam Brahmachari (Ed). Natural Products: Chemistry, Biochemistry
and Pharmacology, Narosa Publishing House Pvt. Ltd., New Delhi, India, pp. 463-519.
proceedings)
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-
6th European Conference on Marine Natural Products, 19-23 July 2009, Porto, Portugal.
A Kijjoa - Chairman. http://www.cimar.org/6ECMNP/
Collaborative Research: the group has collaboration in research work with the following
international institutions:
-
Laboratory of Toxicology - Institute of Pharmacy - Free University of Brussels (Prof.
Robert Kiss).
-
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Thailand
(Prof. Leka Manoch).
-
Department of Chemistry and Biochemistry, The Florida State University, Tallahassee,
USA (Prof. Werner Herz).
-
Bangsaen Institute of Marine Science, Burapha University, Chonburi, Thailand (Dr.
Sumaitt Putchakarn).
Graduate Training: The laboratory of Natural Products Chemistry has received PhD’s students
from Thailand and Brazil for the training on isolation, purification and structure elucidation of
natural products, under the network of mobility programme.
ERASMUS Programme with The Faculty of Pharmacy, Ankara University, Turkey.
ASEM-DUO Network with Burapha University, Bangkok, Thailand.
A Kijjoa is a member of Marine Board Working Group in Marine Biotechnology. He is
coordinating the topics of “Development of Novel Pharmaceutical Products” and
A Kijjoa is a member of Editorial Advisory Board of the Following Journals: Marine Drugs, Journal
of Natural Pharmaceuticals.
A Kijjoa is a reviewer of the following scientific journals: Phytochemistry, Phytochemistry
Letters, Ethnopharmacology, Marine Drugs, Food and Chemical Toxicology.
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A Kijjoa is a member of Marine Board Working Group in Marine Biotechnology. He is
coordinating the topics of “Development of Novel Pharmaceutical Products” and
8e. Future research
1. Objectives

Current funding
10.000 EUR FCT Pluriannual funding.
148.346,00 EUR, FCT PTDC/MAR/100482/2008 - Bioactive products in marine algae of Azores
(AzoAlg), 2009-2011.

130.176,00 EUR, FCT PTDC/MAR/112708/2009, Characterization and Antitumour Activity
Evaluation of Bioactive Compounds from Marine Derived Fung.
129.648,00 EUR, FP7-KBBE-2010-4, SUSTAMAR - Sustainable culture of marine microorganisms,
algae and/or invertebrates for high added value products. Project coordinated by Prof. Peter
Proksch, Institute of Pharmaceutical Biology and Biotechnology, University of Düsseldorf,
Germany.
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Adelino Vicente Mendonca Canario
-
Source: European Commission
366.304 EUR, FP7 project nº 222719, 2009-2012.
-
Source: FCT
25.000 EUR/year, FCT Pluriannual funding.
185.091 EUR, PTDC/CVT/66735/2006, 2007-2010.
10.944 EUR, PTDC/PSI/71811/2006, 2007-2010.
7.200 EUR, PTDC/MAR/72117/2006, 2008-2011.
69.592 EUR, PTDC/MAR/69749/2006, 2008-2011.
18.000 EUR, PTDC/MAR/71351/2006, 2008-2011.
175.504 EUR, PTDC/CVT/104750/2008, 01/2010-12/2012.
165.224 EUR, PTDC/MAR/104008/2008, 1/2010-12/2012.
-
Source: Others
30.000 EUR, several sources and services.
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1. Objectives
The main aim of the group is to study the role of hormones and their metabolites on the
physiology and the evolution of the endocrine system, using fish as the principal model
complemented by studies in other invertebrate and vertebrate models. The research is directed
at four main interrelated themes: 1) Hormonal control of Reproduction and Development; 2)
Calcitropic hormones, mineral metabolism and environmental adaptation, 3) Hormones,
pheromones and behaviour, 4) The impact of Genotype on phenotype. Specific objectives are:
Hormonal control of reproduction and development
-
To establish the identity, function and mechanisms of action of key regulatory factors in
relation to sex differentiation, puberty and gonad maturation.
-
To understand how thyroid hormones drive developmental processes such as
metamorphosisat a cellular to whole organism level.
Calcitropic hormones, mineral metabolism and environmental adaptation
-
To understand the evolution of mineral homeostasis and the role of endocrine
regulating factors, their site of action and the molecular mechanisms responsible for
their biological actions.
-
To understand the process of ossification and regeneration in fishes and the role of the
extracellular matrix, minerals and endocrine factors.
Hormones, pheromones and behaviour
-
To understand the underlying mechanisms regulating the interaction between
hormones and behaviour in fishes.
-
To understand how fish detect biologically important environmental chemical cues pheromones, food-related odorants and inorganic cations (Ca2+ and Na+) and how this
olfactory input is centrally processed to evoke the appropriate physiological and
behavioural responses.
The impact of genotype on phenotype
-
To apply an integrative and systems approach, to understand how the genome impacts
on phenotype and the role of genome evolution in the establishment of the complex
hormones/systems in metazoa.
-
Discovered that an odour present in polichaetes enhances swimming speed and feed
intake in sole - a patent for the compound was filed.
125
-
Discovered that fish have a broad olfactory sensitivity towards bile acids, which could be
related to potential roles in both intra-specific chemical communication and in interspecific interactions.
-
Determined the dynamics of recovery of olfactory neurons when exposed to copper and
found that speed of recovery varies with olfactory neuron type, with food related
neurons recovering faster than pheromone related neurons.
-
A NaPi-III transporter (PiT 1 or PiT 2) has major responsiblity for the transport of
inorganic phosphate (Pi) across the shark choroid plexus.
-
Established the importance of phosphorus over calcium in determining normal skeletal
development and growth and identification of extracellular matrix biomarkers in trout.
-
Confirmed a critical role for calmodulin controlling testicular steroidogenesis and
possibly sex differentiation in tilapia.
-
Identification of genes which are differentially expressed in the fish gill during
adaptation to altered calcium water concentrations, while providing the largest gill
transcriptome description to date.
-
Established the presence of estrogen receptors in fish scales, which suggests a direct
action of estradiol on osteoclasts to bring about calcium mobilizing actions.
-
Established a role for calciotropic hormones in the regulation of acid-base status of
marine fish through their action in the intestine.
-
Characterized the sea bream vertebra proteome and how it is modified by Parathyroid
Hormone related Peptide (PTHrP).
-
Identified novel PTHrP alternative transcripts in chicken and Xenopus.
Technology development /transfer:
-
Developed a new subtractive hybridization technique.
-
Establishment of methodology for establishment of stable cell lines over expressing
transcripts of interest and associated methods to study signaling and gene transcription.
-
Development in consortium of a medium throughput strategy for recombinant protein
production from genes from marine organisms, protocols were established involving
recombinatorial cloning, protein expression screening and batch puriﬁcation.
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8d. Productivity
293(1-2): 100-107. IF=1.925.
2. Deloffre, LAM, Martins, RST, Mylonas, CC, Canario, AVM. 2009. Alternative transcripts of
DMRT1 in the European sea bass: Expression during gonadal differentiation. Aquaculture 293(12): 89-99. IF=1.925.
3. Fontagne, S, Silva, N, Bazin, D, Ramos, A, Aguirre, P, Surget, A, Abrantes, A, Kaushik, SJ, Power,
DM. 2009. Effects of dietary phosphorus and calcium level on growth and skeletal development
in rainbow trout (Oncorhynchus mykiss) fry. Aquaculture 297(1-4): 141-150. IF=1.925.
4. Huertas, M, Hagey, L, Hofmann, AF, Cerdà, J, Canário, AVM, Hubbard, PC. 2009. Olfactory
sensitivity to bile fluid and bile salts in the European eel (Anguilla anguilla), goldfish (Carassius
auratus) and Mozambique tilapia (Oreochromis mossambicus) suggests a 'broad range'
sensitivity not confined to those produced by con-specifics alone. Journal of Experimental
Biology 213: 308-317. IF= 2.722.
5. Kalamarz, H, Nietrzeba, M, Fuentes, J, Martinez-Rodriguez, G, Miguel Mancera, J,
Kulczykowska, E. 2009. Melatonin during larval and post-larval development of gilthead sea
bream Sparus auratus L.: more than time-keeping molecule? Journal of Fish Biology 75: 142-155.
IF= 1.226.
6. Kolmakov, NN, Hubbard, PC, Lopes, O, Canario, AVM. 2009. Effect of acute copper sulfate
exposure on olfactory responses to amino acids and pheromones in goldfish (Carassius auratus).
Environmental Science and Technology 43(21): 8393-8399. IF= 4.630.
7. Laiz-Carrion, R, Fuentes, J, Redruello, B, Guzman, JM, del Rio, MPM, Power, D, Mancera, JM.
2009. Expression of pituitary prolactin, growth hormone and somatolactin is modified in
response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream
Sparus auratus. General and Comparative Endocrinology 162(3): 293-300. IF= 2.732.
8. Martins, RST, Fuentes, J, Almeida, O, Power, DM, Canario, AVM. 2009. Ca2+-Calmodulin
regulation of testicular androgen production in Mozambique tilapia (Oreochromis
mossambicus). General and Comparative Endocrinology 162(2): 153-159. IF= 2.732.
9. Morgado, I, Campinho, MA, Costa, R, Jacinto, R, Power, DM. 2009. Disruption of the thyroid
system by diethylstilbestrol and ioxynil in the sea bream (Sparus aurata), Aquatic Toxicology
92(4): 271-280. IF= 3.124.
10. Oliveira, RF, Silva, A, Canario, AVM. 2009. Why do winners keep winning? Androgen
mediation of winner but not loser effects in cichlid fish. Proceedings of the Royal Society B Biological Sciences 276(1665): 2249-2256. IF= 4.857.
127
11. Passos, ALS, Pinto, PIS, Power, DM, Canario, AVM. 2009. A yeast assay based on the gilthead
sea bream (teleost fish) estrogen receptor beta for monitoring estrogen mimics. Ecotoxicology
and Environmental Safety 72(5): 1529-1537. IF= 2.133
12. Pinto, PIS, Estevao, MD, Redruello, B, Socorro, SM, Canario, AVM, Power, DM. 2009.
Immunohistochemical detection of estrogen receptors in fish scales. General and Comparative
Endocrinology 160(1): 19-29. IF= 2.732.
13. Saraiva, JL, Barata, EN, Canario, AVM, Oliveira, RF. 2009. The effect of nest aggregation on
the reproductive behaviour of the peacock blenny Salaria pavo. Journal of Fish Biology 74(4):
754-762. IF= 1.226.
14. Velez, Z, Hubbard, PC, Barata, EN, Canario, AVM. 2009. Adaptation to reduced salinity affects
the olfactory sensitivity of Senegalese sole (Solea senegalensis Kaup 1858) to Ca2+ and Na+ but
not amino acids. Journal of Experimental Biology 212(16): 2532-2540. IF=2.196.
15. Velez, Z, Hubbard, PC, Welham, K, Hardege, JD, Barata, EN, Canario, AVM. 2009.
Identification, release and olfactory detection of bile salts in the intestinal fluid of the
Senegalese sole (Solea senegalensis), Journal of Comparative Physiology A-Neuroethology
Sensory Neural and Behavioral Physiology 195(7): 691-698. IF= 2.196.
proceedings)
Books (Chapters)
1. Barata, EN, Gonçalves, DM. 2009. Communication in Blennies. In: Patzner, RA, Gonçalves, EJ,
Hastings, PA, Kapoor, BG (Eds). The Biology of Blennies, Enfield, USA, Science Publishers Inc., pp.
351-378. ISBN 978-1-57808-439-5.
2. Power, DM, Morgado, I, Cardoso, JCR. 2009. Evolutionary Insights from fish transthyretin. In:
Richardson, SJ, Cody, V. Recent Advances in Transthyretin Evolutions, Structure and Biological
Functions, vol 1, 360 pp., Springer.
Conference proceedings and abstracts
3. Anjos, L, Redruello, B, Gomes, A, Melo, E, Canario, AVM, Power, DM. 2009. Acidic and
Secreted Protein in Pituitary (ASPIP) - Biochemical and biophysical charactrerization of a novel
pituitary protein. Proceedings of the XXXVIth International Union of Physiological Sciences
(IUPS). The Journal of Physiological Sciences 59 (Supp 1).
4. Canario, AVM, Pinto, PIS, Reinhardt, R, Matsumura, H. 2009. Gill transcriptome changes under
different conditions of water calcium availability in Tetraodon nigroviridis. Proceedings of the
XXXVIth International Union of Physiological Sciences (IUPS). The Journal of Physiological
Sciences 59 (Supp 1): 536.
5. Cardoso, JCR, Coelho, N, Power, DM. 2009. Crosstalk between the metazoan family 2 GPCR
system. Proceedings of the XXXVIth International Union of Physiological Sciences (IUPS). The
Journal of Physiological Sciences 59 (Supp 1): 98.
128
6. Pinheiro, P, Downie, H, Fuentes, J, Power, DM, Canario, AVM. 2009. Functional activity and
gene expression of parathormone. Proceedings of the XXXVIth International Union of
Physiological Sciences (IUPS). The Journal of Physiological Sciences 59 (Supp 1): 273.
7. Pinto, PIS, Power, DM, Canario, AVM, M. A. S. Thorne, R. Reinhardt, H. Matsumura & R.
Terauchi (2009), Transcriptome profiling of the gills of a euryhaline teleost fish, Tetraodon
nigroviridis, in response to altered calcium concentrations in water. Proceddings of the 26th
Congress of the European Society of Comparative Biochemistry and Physiology (ESCBP) Environmental stimuli and their impact on cellular homeostasis and gene regulation.
Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology 154(1): S2-S3.
8. Velez, Z, Hubbard, PC, Welham, KJ, Barata, EN, Hardege, JD, Canário, AVM. 2009. Functional
asymmetry in the olfactory system of a flatfish, the Senegalese sole (Solea senegalensis).
Proceedings of the Annual Meeting of the Society for Experimental Biology. Comparative
Biochemistry and Physiology A - Molecular and Integrative Physiology 153(2): S90.
Symposium. European Aquaculture Society, Special Publication nº38: 362-365.
proceedings)
MASTER THESES
Cabrita, CI. 2009. Functional characterisation of novel neuropeptides in vertebrates. Master
thesis in Biological Engeneering, University of Algarve. Supervisors: J Cardoso and DM Power.
Campos do Mar, A. 2009. Caracterização endócrina da PTH-L e calcitonina no transporte de
cálcio e na secreção de bicarbonato no intestino da dourada (Sparus auratus). Master thesis in
Marine Biology, University of Algarve. Supervisors: DM Power and J Fuentes.
Carvalho, E. 2009. Disrupção da glândula tiróide pelo composto goitrogeno (PTU): estudo de
aproximação molecular e morfo-funcional da tiróide e pele de Xenopus laevis. Master thesis in
Coelho, NA. 2009. Análise e caracterização da expressão e papel funcional de potenciais genes
da família 2 GPCRs no protostómio Caenorhabditis elegans. Master thesis in Biotechnology,
University of Algarve. Supervisors: J Cardoso and DM Power.
Mazagão, E. 2009. Thyroid Axis disruption by goitrogens: a molecular and functional approach.
Integrated Master thesis in Biological Engeneering, University of Algarve. Supervisors: DM
Power and P Pinto.
129
Pereira, D. 2009. Efeito da anosmia na endocrinologia e reprodução de machos de Oreochromis
mossambicus (Peters, 1852). Master thesis in Marine Biology, University of Algarve. Supervisors:
T Modesto and PC Hubbard.
Santos, C. 2009. Alterações histológicas nas brânquias e no rim do xarroco, Halobatrachus
didactylus (Bloch & Schneider, 1801), exposto a concentrações elevadas de amónia. Master
thesis, University of Algarve. Supervisors: T Modesto and M Huertas.
PhD THESES
Canario, Co-supervisor: J Coimbra.
University of Porto. Supervisor: AVM Canario, Co-supervisor: J Coimbra.
thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Rui Oliveira (ISPA), Cosupervisors: AVM Canario and J Coimbra.
Velez, Z. 2009. Neural mechanisms of olfactory discrimination in the Senagalese sole, Solea
senegalensis Kaup, 1858. PhD thesis, University of Évora. Supervisors: EN Barata and PC
Hubbard, Co-supervisor: AVM Canario.
Feed additives and their use for aquaculture and aquiculture. Provisional patent 104732 under
approval by the Portuguese patent office.
Negotiations ongoing (Denmark) for studies of alpha-ketoglutarate.
In 2009 the group published with colleagues from 14 countries and 15 institutions and research
institutes mainly from Europe. An important advance this year was funding secured with a
consortium of countries from the Atlantic region for translational research with the objective of
making technology more accessible to Industry. In the context of Aquagenome FP6 specific
support action mobility grants for young scientists was successfully administered and 1 fellow (T
Ibarez) was hosted by the research group. The group leader and CCMAR director was
instrumental in contributing to the centre international standing with participation in several
institutional applications to FP7: MG4EU (dissemination of genomics in marine sciences); EMBRC
130
(ESFRI road map project for setting up an European marine Biology Resource Centre) and
Euromarine (a consortium of the three FP6 networks of Excellence in Marine Sciences. Group
members participate in COST Action network 867 dedicated to “Welfare of Fish in European
Aquaculture” (P Guerreiro). During 2009 group members were invited to write reviews (n=5) for
several books/ journals and give lectures: e.g. Genetics in Aquaculture meeting held in Bodo,
Norway in July 2009. DM Power is Vice President of the Iberian association of Comparative
Endocrinology. DM Power was invited to be part of the European Technology Platform (EATIP
Thematic Area 3, Managing the biological lifecycle). Researchers from Spain (Univ Murcia, Univ
Barcelona, Univ Cadiz), Sweden (Univ Goteborg), UK (British Antarctic Survey, University of Hull)
and Brazil (Univ Fed Rio Grande do Sul) made research visits to our group (1 week – 5 months).
Researchers from our group made research visits to Sweden (Univ Goteborg), Brazil (Univ Fed
Rio Grande do Sul), Spain (Univ Murcia), UK, (University of Bath, University of Edinburgh) Italy
(Univ Padova) for periods of 1 week to 3 months. A PhD student was awarded a Marie Curie
fellowship to study in the University of Edinburgh for one year. DM Power is associated editor of
General Comparative Endocrinology and the Journal of Pre-Clinical and Clinical Research; AVM
Canario is Advisory editor of Acta Ethologica and Top Reviewer 2009 for General and
Comparative Endocrinology. Senior members of the group reviewed more than 80 manuscripts
for international journals. Reviewed project and Marie Curie applications for the European
Commission and programmes in several countries (Argentina, France, Spain, UK, The
Netherlands, USA).
The CME group collaborated in the preparation of a white paper for Aquaculture in the context
of Aquagenome which resulted in a report which was divulged to the European Commission and
other stakeholders. Similarly a “mind-pooling” exercise was conducted and the CME group was
involved in determining how the results should be reported to industry and used to generate a
road map for implementation of genomics tools in aquaculture. Contract research was carried
out in the context of a Norwegian Aquaculture research project. The expertise of the CME group
was sought for radioimmunoassay and analysis of other endocrine parameters.
8e. Future research
1. Objectives
The objectives below are medium term (5 years), for which we have either secured funding or
have applied for funding:
-
-
What genes determine sex in fish? The focus is on the identification of early genes of the
cascade of sex differentiation using transcriptomic and transgene approaches, with a
view of understanding how environmental factors such as temperature can modify sex
ratios.
What signals direct fish to grow or to mature? There are metabolic, behavioural and
neuroendocrine components which need to be dissected using global transcriptomics
131
-
-
-
-
-
-
approaches to help us understand the mechanisms underlying the development of
alternative mating tactics in fish and other vertebrates as well as precocious puberty in
farmed fish.
Develop a model system for characterisation of TH on organogenesis and functional
modifications during fish metamorphosis in order to study how asymmetry is generated.
Establish the role of TH receptors, deiodinases and TH binding proteins and cell
transporter proteins in regulating TH tissue responsiveness during fish metamorphosis.
To establish a model for the mechanism of action and physiological roles of the
parathyroid (PTH) family of peptides in fish. Identification of signaling pathways,
downstream responsive genes in key ion regulatory tissue (bone, intestine and kidney)
and upstream biological responses. Physiological techniques, transcriptome and
proteome analysis, RNA interference and transgenic approaches will be used.
Deorphanisation and functional characterisation of potential calcitonin/calcitonin
related peptide and PTH-like proteins and their receptors in invertebrates and
vertebrates. The approach includes physiological studies, RNA interference, gene
expression and ligand peptide screening.
Establish how pheromones regulate reproductive and social interactions in fish by
determining the chemical nature of pheromones, site of production and regulation of
synthesis and their mode of action. Electrophysiology, behaviour, chemistry and
genomics approaches will be used to study this question. Identification of ligands for
olfactory receptors, the signaling pathways and mapping of olfactory neurones to
olfactory bulb centers are some of the expected outcomes.
Identification of odorants present in natural diets of economically important fish, which
may be used as attractants in additives to inert-feed for improvement of feed ingestion,
growth and feed conversion rates in aquaculture. This work is particularly directed at
Solea senegalensis, a new species for aquaculture for which feeding and the production
of attractive dry diets is a bottleneck.
Characterisation of the role of BMP-15 and GDF9 in the oocyte maturation. This work
will include receptor identification and functional characterization by loss and gain of
function analysis (siRNA or mRNA microinjection). New genes regulated by BMP-15 and
potentially involved in the oocyte maturation will also be identified using the
mozambique tilapia, Oreochromis mossambicus, as a model.

Current funding
-
Source: European Commission
366.304 EUR, FP7 project nº 222719, 2009-2012.
-
Source: FCT
25.000 EUR/year, FCT Pluriannual funding.
185.091 EUR, PTDC/CVT/66735/2006, 2007-2010.
10.944 EUR, PTDC/PSI/71811/2006, 2007-2010.
132
7.200 EUR, PTDC/MAR/72117/2006, 2008-2011.
69.592 EUR, PTDC/MAR/69749/2006, 2008-2011.
18.000 EUR, PTDC/MAR/71351/2006, 2008-2011.
175.504 EUR, PTDC/CVT/104750/2008, 01/2010-12/2012.
165.224 EUR, PTDC/MAR/104008/2008, 1/2010-12/2012.
-
Source: Others
30.000 EUR, several sources and services

151.056 EUR, PTDC/MAR/108063/2008.
147.852 EUR, PTDC/MAR/099893/2008.
162.282 EUR, PTDC/CVT/102178/2008.
185.099 EUR, PTDC/MAR/113353/2009.
167.412 EUR, PTDC/MAR/110264/2009.
159.391 EUR, PTDC/MAR/112311/2009.
164.399 EUR, PTDC/CVT/114609/2009.
150.586 EUR, PTDC/BIA-BIC/114340/2009.
166.108 EUR, PTDC/BIA-BIC/113552/2009.
143.310 EUR, PTDC/MAR/115005/2009.
131.692 EUR, PTDC/MAR/115994/2009.
133
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)
Luis Manuel Zambujal Chicharo
Funding in 2009:
60.000 EUR, INTERREG, 0252_DIMEAGUA_5_P, Development and harmonization of new
indicators, methodologies and strategies common for Portugal and Spain for the application of
the Water Frame Directive to transitional and coastal water mass in the Guadiana. 2/20091/2011
199.962,90 EUR, ACID-BIV, The integrated impacts of marine acidification, temperature and
precipitation changes on coastal biodiversity and fisheries: how to adapt? Funded by the ERANET CIRCLE MED - FCT Portugal (Italian Ministry for Environment, Land and Sea - IMELS, Italy;
Regional Ministry of Innovation and Industry of the Galician Government trough the Directorate
General for Research, Development and Innovation - XUNTA, Spain). 10/2008-9/2010
8.000 EUR, FCT/MESRST, GUAMED - A comparative study between Guadiana and Medjerda
coastal zones. Bilateral collaborative project Portugal-Tunisia. Funding for 8 short scientific visits
(1 week each) exchange between researchers from both institutions (CCMAR and Sciences
Faculty of Bizerta –FSB), 10/2008-9/2010
48.510,00 EUR, FCT PTDC/MAR/098643/2008, MODELA - Modelação da dinâmica larvar de
peixes e processos oceanográficos relevantes, 4/2010-2/2013
134
1. Objectives
Our research is mainly focused on biological and physical-chemical processes in transitional
waters, from freshwater catchments to coastal marine ecosystems. We are particularly
interested in primary and secondary production, nutrient cycles, the characterisation of
habitats, populations, recruitment, trophic interactions and community dynamics. The purpose
of our research is to deeply understand the natural variability of aquatic ecosystems (e. g.,
freshwater inputs, tides, upwelling, winds) and the ecologic impacts by caused by human
activities (fisheries, pollution, dams, alien species, etc). All this information is necessary to
develop and test Ecohydrology ecosystem-based solutions for reverting degradation, sustaining
functioning and promoting adaptation of aquatic environments to impacts, considering the
current scenarios for climatic changes.
In the field of estuarine ecology an understanding the threats of invasive species to estuarine
ecosystems, especially in the Guadiana estuary, was achieved. The routine determination of
Secondary production in this estuary by copepods egg methods was also accomplished.
In the field of ecology of fish larvae the major achievement of the group was the analysis of the
RNA:DNA ratios as condition indicator and its relation with swimming abilities of larvae and its
implications to recruitment.
In the field of global changes it was set up an experimental approach to analyse the effects of
marine acidification on invertebrates, such as bivalves. Results with Ruditapes decussatus and
Mytilus edulis show that physiological parameters are particularly affected, this was achieved
with biochemical indices such as RNA:DNA and RNA:protein ratios, and physiological rates, such
as respiration and excretion rates.
8d. Productivity
1. Chícharo, L, Ben Hamadou, R, Amaral, A, Range, P, Mateus, C, Piló, D, Marques, R, Chícharo,
MA. 2009. Application and demonstration of the Ecohydrology approach for the sustainable
functioning of the Guadiana estuary (South Portugal). Ecohydrology and Hidrobiology 9(1): 5571.
2. Chicharo, MA, Leitão, T, Range, P, Gutierrez, C, Morales, J, Morais, P, Chícharo, L. 2009. Alien
species in the Guadiana Estuary (SE-Portugal/SW-Spain): Blackfordia virginica (Cnidaria,
Hydrozoa) and Palaemon macrodactylus (Crustacea, Decapoda): potential impacts and
mitigation measures. Aquatic Invasions 4(3): 501-506.
135
3. Chícharo, L, Faria, A, Morais, P, Amaral, A, Mendes, C, Chicharo, MA. 2009. How to sample
larval fish for taxonomical and ecophysiological studies in shallower temperate coastal
ecosystems? Les Cahiers de Biologie Marine 50(4): 311-318. IF=0.873.
4. Leitao, A, Vasconcelos, P, Ben-Hamadou, R, Gaspar, MB, Barroso, CM, Ruano, F. 2009.
Cytogenetics of Bolinus brandaris and phylogenetic inferences within the Muricidae (Mollusca:
Gastropoda). Biological Journal of the Linnean Society 96: 185-193. IF=2.040.
5. Morais, P, Faria, A, Chicharo, MA, Chicharo, L. 2009. The unexpected occurrence of late
Sardina pilchardus (Walbaum, 1792) (Osteichthyes: Clupeidae) larvae in a temperate estuary.
Les Cahiers de Biologie Marine 50(1): 79-89. IF=0.873.
6. Morais, P, Chicharo, MA, Chicharo, L. 2009. Changes in a temperate estuary during the filling
of the biggest European dam. Science of the Total Environment 407: 2245-2259. IF=2.905.
7. Olivar, PM, Diaz, MV, Chicharo, MA (2009). Tissue effect on RNA:DNA ratios of marine fish
larvae Scientia Marina 73(S1): 171-182. IF=1.174.
proceedings)
Books
1. Chicharo, L, Wagner, I, Chicharo, M, Lapsinka, M, Zalewski, M. 2009. Practical experiments
guide for Ecohydrology. In: Chicharo et al. (Eds). UNESCO Manual, Faro, Portugal, 121 pp, ISBN:
978-989-20-1702-0.
Books (Chapters)
2. Ben-Hamadou, R. 2009. Modelling estuarine ecological response to diverse hydrological
patterns. Bottom-up control. In: Chicharo et al. (Eds), Practical experiments guide for
Ecohydrology. UNESCO Manual, Faro, Portugal, pp. 65-68, ISBN: 978-989-20-1702-0.
3. Chicharo, L. 2009. May bivalves be used to control toxic algae blooms? In: Chicharo et al.
(Eds), Practical experiments guide for Ecohydrology. UNESCO Manual, Faro, Portugal, pp. 55-58,
ISBN: 978-989-20-1702-0.
4. Chicharo, MA. 2009. Are males specimens more adequate to detect antropogenic impacts?.
In: Chicharo et al. (Eds), Practical experiments guide for Ecohydrology. UNESCO Manual, Faro,
Portugal, pp. 79-100, ISBN: 978-989-20-1702-0.
proceedings)
136
MASTER THESES
Mateus, C. 2009. Remoção de nutrientes de águas eutrofizadas através de Salicornia ramossima.
Master Thesis, Marine Biology - Ecology and Conservation, University of Algarve. Supervisor: L
Chicharo, Rui Santos (ALGAE Group, CCMAR).
Maximo, I. 2009. Avaliação da qualidade da água das ribeiras do Algarve: uso de
macroinvertebrados bentónicos como bioindicadores. Master thesis, Marine Biology - Ecology
and Conservation, University of Algarve. Supervisor: L Chicharo, MA Chícharo.
Sá, E. 2009. Control of the eutrophication process in aquatic ecosystems. Master thesis, Marine
Biology - Ecology and Conservation, University of Algarve. Supervisor: L Chicharo, Radhouan
Ben-Hamadou.
PhD THESES
Amaral, A. 2009. Aquaculture impact of clam Ruditapes decussatus in the Ria Formosa: effects
on the ecosystems and species physiology. PhD thesis, University of Santiago de Compostela,
Spain. Supervisor: L Chicharo, Co-supervisor Marisa José Reiriz (CSIC, Vigo, Spain).
BIOLIEF - World Conference on Biological Invasions and Ecosystem Functioning, 27-30 October
2009, Porto, Portugal. Members of Scientific Committee.
Collaborative publication
-
Chicharo, L, Wagner, I, Chicharo, M, Lapsinka, M, Zalewski, M. 2009. Practical
experiments guide for Ecohydrology. In: Chicharo et al. (Eds). UNESCO Manual, Faro,
Portugal, 121 pp, ISBN: 978-989-20-1702-0. This publication was developed based on
collaborative research between ERCE (Ecohydrology center), Poland and CCMAR.
-
Chicharo, MA, Leitão, T, Range, P, Gutierrez, C, Morales, J, Morais, P, Chícharo, L. 2009.
Aquatic Invasions 4: 3: 501-506. This publication was developed based on collaborative
research between Instituto Andaluz de Investigación y Formación Agraria, Pesquera,
137
Alimentaria y de la Producción Ecológica (IFAPA) (Centro “Agua del Pino”) Huelva
CCMAR.
-
Olivar, PM, Diaz, MV, Chicharo, MA. 2009. Tissue effect on RNA:DNA ratios of marine
fish larvae Scientia Marina 73(S1): 171-182. IF=1.075. This publication was developed
based on collaborative research between Institut de Ciències del Mar, Spain, Instituto
Nacional de Investigación y Desarrollo Pesquero, Argentina and CCMAR.
Graduate Training Networks
-
Bilateral collaborative project FCT/MESRST project (Portugal-Tunisia): Coastal ecology
and global changes. A comparative study between Guadiana and Medjerda coastal
zones (GUAMED). Funding for 8 short scientific visits (1 week each) exchange between
researchers from both institutions (CCMAR and Sciences Faculty of Bizerta - FSB).
-
MedZoo: MedZoo is an association of scientists willing to collaborate on the
harmonisation of methods for study of the evolution of Mediterranean and Black Sea
zooplankton in time and space. Medzoo was created during the 37th CIESM congress in
Barcelona in June 2004 and is functioning through the CIESM program on Zooplankton
Indicators.
-
WssTP: The Water supply and sanitation Technology Platform (WssTP) is a European
initiative for European Research and Technology Development in the water industry.
Collaborative projects
-
Research Project ACID BIV - CIRCLE-MED, coordinated by CCMAR (Luis Chícharo), with
the following partners: Consejo Superior de Investigaciones Cientificas (CSIC), Vigo,
Spain; Department of Biology - University of Padova; Faculty of Science of Bizerta,
Tunisia.
-
Research INTERREG project DIMEAGUA, coordinated by CCMAR (Luis Chícharo), with the
following partners: Instituto Andaluz de Investigación y Formación Agraria, Pesquera,
Alimentaria y de la Producción Ecológica (IFAPA) Centro “Agua del Pino”.
8e. Future research
1. Objectives
Global change in coastal ecosystems
-
138
Impacts of alien species on coastal ecosystems, and effects marine acidification on
fisheries and aquaculture.
Fish larvae trophodynamics
-
New trophic links between fish larvae and plankton will be also a topic for research,
especially alternative links between bacteria-phytoplankton and ciliates, instead of
traditional phyto-nauplii-fish larvae links.
Ecohydrology and ecosystem services in estuaries and coastal areas
-
The projected research is intended to be formally associated with the activity of a future
International Centre for Coastal Ecohydrology (ICCE) under the UNESCO auspices, at the
University of Algarve and having as an objective a close collaboration in fomenting and
better disseminate research in coastal sciences with the CIMAR LA. This collaboration
would enhance the internationalization of the produced research and applications and
build synergetic partnership with regional and international water related stakeholders.

Current funding
60.000 EUR, INTERREG, 0252_DIMEAGUA_5_P, Development and harmonization of new
indicators, methodologies and strategies common for Portugal and Spain for the application of
the Water Frame Directive to transitional and coastal water mass in the Guadiana, 2/20091/2011.
199.962,90 EUR, ERA-NET CIRCLE MED, ACID-BIV, The integrated impacts of marine acidification,
temperature and precipitation changes on coastal biodiversity and fisheries: how to adapt?,
10/2008-9/2010.
8.000,00 EUR, FCT/MESRST, GUAMED - A comparative study between Guadiana and Medjerda
coastal zones. Bilateral collaborative project Portugal-Tunisia, for 8 short scientific visits (1 week
each) exchange between researchers from both institutions (CCMAR and Sciences Faculty of
Bizerta - FSB), 10/2008-9/2010.
48.510 EUR, FCT PTDC/MAR/098643/2008, MODELA - Modelação da dinâmica larvar de peixes e
processos oceanográficos relevantes, 4/2010-2/2013.

156.005 EUR, FCT PTDC/BIA-BIC/113157/2009, New trophic link in early phases of estuarine
fishes.
149.318 EUR, FCT PTDC/MAR/112382/2009, Update knowledge of ecophysiological condition in
early phases of estuarine fishes to face climate changes. Requested funding:
75.534 EUR, FCT PTDC/MAR/115795/2009, The effects of Ocean Acidification on two calanoid
copepod species: multi-generational experiments, OA-Copepod.
139
130.720 EUR, FCT PTDC/AAC-AMB/116010/2009, Evaluation of Guadiana wetland nursery
functions using advanced modelling techniques, WETNURSE.
140
Ecophysiology
Ecophysiology
CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto
Funding spent in 2009:
-
31.500 EUR, FCT Pluriannual funding
-
National funding
31.800,00 EUR, FCT PTDC/AMB/70431/2006
36.980,03 EUR, FCT PTDC/MAR/64016/2006
-
European funding
31.728,76 EUR, INTERREG IV POCTEP, NATURA-MINHO-MIÑO
3.926,95 EUR, 6 FP-EU, AMPERA
-
Private funding
60.576,06 EUR, EDP - Energy sector company, Biodiversity fund - National plan for the
conservation of lamprey (Lampetra fluviatilis and Lampetra planeri) (Plano nacional de
conservação da lampreia-de-rio e da lampreia-de-riacho). Total funding: 115.000 EUR, 20092011.
141
1. Objectives
-
Within the context of conservation physiology to investigate the physiological
challenges faced by migratory fishes (Anguilla anguilla, Platichthys flesus, Petromyzon
marinus, Scyliorhinus cannicula, Pagellus bogaraveo, Trachurus trachurus, Sardinia
pilchardus, Spondyliosoma cantharus, Diplodus vulgaris), focusing on the abiotic factors
of salinity and hydrostatic pressure. The species that are investigated are of commercial
and/or biological significance.
-
Determine migratory patterns of fishes (A. anguilla, Conger conger, P. flesus) at different
life history stages using otolith fingerprinting and complementary techniques.
-
Defining the mechanisms for dealing with physiological constraints and challenges of
ammonotelism in fishes. Ammonia can be highly toxic to animals yet is a natural
byproduct of metabolism.
-
Elucidate the mechanism of shell calcification in freshwater bivalves (Anodonta cygnea).
Implication of climatic changes on the whole biomineralization mechanism in bivalves.
Evaluate the immune responses to the toxicological effects and pathogenic agents in
bivalves. Characterize the main ecophysiological parameters of Portuguese species of
freshwater bivalves. Define of optimum culture conditions and new conservation
methods for freshwater bivalves (Unionoida).
-
Technological advances to aid in biological discovers and monitoring of the
environment. Specifically the development of methods for the non-invasive monitoring
of physiological parameters and the development of hyperbaric systems for the
maintenance of fish and other aquatic organisms for investigating hydrostatic pressure
effects on physiology.
-
Determination of the physiological impact of toxicant exposure and environmental
changes through the measurement of physiological and biochemical endpoints
(biomarkers). This includes the application of cDNA microarrays to study transcriptome
level changes in response to hydrostatic pressure.
-
Determination of bivalve reproduction strategies and the effects of hydrostatic pressure
and seasonal changes on shell calcification.
-
Elucidation of mechanisms of salinity tolerance in euryhaline fishes and the interaction
with nitrogen regulatory mechanisms.
-
Inclusion in EU level networks and participation in other national and international
collaborative projects.
-
Dissemination of results generated by the group. In 2009 we collectively have 21
international peer reviewed publications, 10 conference presentations, 5 of which were
142
paper presentations. Group members have also been actively involved in education of
the public.
-
Research training at the undergraduate and graduate levels (4 MSc, 4 PhD completed in
2009).
8d. Productivity
1. Baker, DW, Morgan, JD, Wilson, JM, Matey, V, Hueng, K, Brauner, CJ. 2009. White sturgeon,
Acipenser transmontanus, defend intracellular pH during the transient and prolonged
extracellular pH depression induced by hypercarbia American Journal of Physiology 296: R1868R1880. IF=3.661.
2. Cardoso, JFMF, Witte, JI, van der Veer, HW. 2009. Reproductive investment of the American
razor clam Ensis americanus in the Dutch Wadden Sea. Journal of Sea Research 62: 295-8.
IF=1.803.
3. Cardoso, JFMF, Witte, JIJ, van der Veer, HW. 2009. Differential reproductive strategies of two
bivalves in the Dutch Wadden Sea. Estuarine Coastal and Shelf Science 84: 37-44. IF=1.970.
4. Chew, SF, Tng, YYM, Wee, NLJ, Wilson, JM, Ip, YK. 2009. Nitrogen metabolism and branchial
osmoregulatory acclimation in the juvenile marble goby, Oxyeleotris marmorata, exposed to
seawater. Comparative Biochemistry and Physiology 154A (3): 360-369. IF=1.709.
5. Correia, AT, Manso, S, Coimbra, J. 2009. Age, growth and reproductive biology of the
European conger eel (Conger conger Linnaeus, 1758) from the Atlantic Iberian waters. Fisheries
Research 99: 196-202. IF=2.196.
species based on Dynamic Energy Budgets. Journal of Sea Research 62:75-82. IF=1.803.
7. Gravel, A, Wilson, JM, Neto Pedro, DF, Vijayan, MM. 2009. Non-steroidal anti-inflammatory
drugs affect seawater acclimation in rainbow trout. Comparative Biochemistry and Physiology
149C(4): 481-90. IF=2.582.
8. Kopecka-Pilarczyk, J, Correia, AD. 2009. Biochemical response in gilthead seabream (Sparus
aurata) to in vivo exposure to a mix of selected PAHs. Ecotoxicology and Environmental Safety
72 (4): 1296-1302. IF=2.133.
aurata) to in vivo exposure to pyrene and fluorene. Journal of Experimental Marine Biology and
Ecology 372: 49-57. IF=2.116.
143
10. Kovitvadhi S, Kovitvadhi U, Sawangwong P, Trisaranuwatana P, Machado J. 2009.
Morphometric relationship of weight and size of cultured freshwater pearl mussel, Hyriopsis
(Limnoscapha) myersiana, under laboratory conditions and earthen pond phases. Aquaculture
International 17:57-67. IF=0.753.
11. Lopes-Lima, M, Lopes, A, Casaca, P, Nogueira, I, Checa, A, Machado, J. 2009. Seasonal
variations of pH, pCO(2), pO(2), HCO3 (-) and Ca2+ in the haemolymph: implications on the
calcification physiology in Anodonta cygnea. Journal of Comparative Physiology B - Biochemical
Systemic and Environmental Physiology 179: 279-86. IF=1.607.
12. Machado, J, Lopes-Lima, M, Damasceno-Oliveira, A, Colaco, A, Andrade, J, Silva, D, JimenezLopez, C, Rodriguez-Navarro, A, Checa, A. 2009. The influence of hydrostatic pressure on shell
mineralization of Anodonta cygnea: a comparative study with a hydrothermal vent bivalve
Bathymodiolus azoricus. Journal of Shellfish Research 2(4): 899-904. IF=0.891.
13. Moreira-Silva, J, Coimbra, JC, Wilson, JM. 2009. Ammonia sensitivity of the glass eel (Anguilla
anguilla L.): Salinity dependence and the role of branchial Na+/K+(NH-4+)-ATPase.
Environmental Toxicology & Chemistry 28: 141-147. IF=2.565.
14. Neves, J, Wilson, JM, Rodrigues, P. 2009. Transferrin and ferritin response to bacterial
infection: the role of the liver and brain in fish. Developmental and Comparative Immunology
33(7): 848-857. IF=3.290.
15. Ozorio, ROA, Valente, LMP, Correia, S, Pousao-Ferreira, P, Damasceno-Oliveira, A, Escorcio,
C, Oliva-Teles, A. 2009. Protein requirement for maintenance and maximum growth of twobanded seabream (Diplodus vulgaris) juveniles. Aquaculture Nutrition 15(1): 85-93. IF=1.482.
16. Peh, WYX, Chew, SF, Wilson, JM, Ip, YK. 2009. Branchial and intestinal osmoregulatory
acclimation in the four-eyed sleeper, Bostrychus sinensis (Lacepède), exposed to seawater.
Marine Biology 156: 1751-1764. IF=1.999.
17. Reis, PA, Antunes, JC, Almeida, CMR. 2009. Metal levels in sediments from the Minho
estuary salt marsh: a metal clean area? Environmental Monitoring and Assessment 159: 191205. IF=1.356.
18. Solé, M, Kopecka, J, Blasco, J. 2009. Pollution biomarkers in two estuarine invertebrates
Nereis diversicolor and Scrobicularia plana from the Cano Sancti-Petri Marsh in the SW Spain.
Environment International 35: 523-531. IF=4.786.
19. Tsui, TKN, Hung, CCY, Nawata, MC, Wilson, JM, Wright, PA, Wood, CM. 2009. Ammonia
transport in gill epithelium of freshwater rainbow trout: the importance of Rhesus glycoproteins
and the presence of an apical Na+/NH4+ exchange complex. Journal of Experimental Biology
212: 878-892. IF=2.722.
20. van der Veer, HW, Cardoso, JFMF, Peck, MA, Kooijman, SALM. 2009. Physiological
performance of plaice Pleuronectes platessa (L.): A comparison of static and dynamic energy
budgets. Journal of Sea Research 62: 83-92. IF=1.803.
144
In press
21. McGuire, A, Aluru, N, Takemura, A, Weil, R, Wilson, JM, Vijayan, MM. (in press).
Hyperosmotic shock adaptation by cortisol involves upregulation of branchial osmotic stress
transcription factor 1 gene expression in Mozambique Tilapia. General and Comparative
Endocrinology, Doi:10.1016/j.ygcen.2009.07.016, published on-line on July 2009.
proceedings)
2. van der Veer, HW, Cardoso, JFMF, Kooijman, SALM. 2009. From Static Energy Budgets (SEB) to
Dynamic Energy Budgets (DEB), linking physiology and ecology, illustrated for plaice
Pleuronectes platessa (L.). Comparative Biochemistry and Physiology A-Molecular & Integrative
Physiology 153A: S148. IF=2.196.
proceedings)
MASTER THESES
P Rema, Co-supervisor: JF Gonçalves.
University of the Algarve. Supervisor: A Correia, Co-supervisor: K Erzini.
Leitão, AF. 2009. Caracterização dos genes Gase, GSase e GDH em Misgurnus anguillicaudatus.
Master thesis in Marine Biology, University of Aveiro. Supervisor: JM Wilson, Co-supervisor: VM
dos Santos Quintino (University of Aveiro).
Supervisor: A Correia, Co-supervisor: K Erzini.
145
Silva, M. 2009. Contribuição do estudo da microestrutura e microquímica de otólitos de
singnatídeos para a Ecologia das espécies, Syngnathus abaster e Nerophis lumbriciformes.
Master thesis in Applied Ecology, Faculty of Science, University of Porto. Supervisor: A Correia,
Co-supervisor: N Monteiro.
PhD THESES
Canario, Co-supervisor: J Coimbra.
University of Porto. Supervisor: AVM Canario, Co-supervisor: J Coimbra.
Moreira da Silva, J. 2009. Ammonia tolerance in the teleost fish Misgurnus anguillicaudatus.
Surviving high ammonia and aerial exposure. PhD thesis in Aquatic Sciences, ICBAS, University of
Porto. Supervisor: J Coimbra, Co-supervisor: JM Wilson.
thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Rui Oliveira (ISPA), Cosupervisors: AVM Canario and J Coimbra.
-
2009, Porto, Portugal. Organising Committe. http://www.ciimar.up.pt/7aiec/index.html
-
October, Porto, Portugal. C Antunes - Member of the Organising Committee.
http://www.ciimar.up.pt/biolief/
Contract research with:
-
EDP, Energy sector company, Biodiversity fund, for the project National plan for the
conservation of lamprey (Lampetra fluviatilis and Lampetra planeri) (Plano nacional de
conservação da lampreia-de-rio e da lampreia-de-riacho). Total funding of 115.000 EUR.
In 2009, members of the Ecophysiology group have been involved in collaborative research with
partners in the Netherlands, the United Kingdom, Spain, Canada, the United States, Australia,
Thailand, and Singapore. Collectively, this has produced 14 publications and five congress
146
communications. A Correira is in collaboration with Dr. A Sial (NEGLABISE, University of
Pernambuco Brazil) and Dr. P Hamer, (Univ Victoria Australia), in nationally funded projects to
perform otolith isotopic and elemental analyses for stock discrimination purposes of some
Portuguese fish species. JM Wilson is involved in collaborative research with Dr. CJ Brauner of
the University of British Columbia and Dr. RW Wilson (University of Exeter) funded by the
Natural Sciences and Research Council (Canada) and British Biological Sciences Research Council
(BBSRC, UK), respectively. We also currently have the post-doctoral researchers A DamascenoOliveira and JFMF Cardoso with cross appointment to the Liverpool Microarray Facility and
Centre for BioArray Innovation, University of Liverpool with Dr. A Cossins and the Royal
Netherlands Institute for Sea Research (NIOZ) with Dr. Henk van der Veer, respectively. Through
provisions in their FCT fellowships, PhD students have worked abroad and members have
participation in the Marine Science and Environment PhD program advanced training offered in
part through CIMAR.
Ecophysiology Group participated in the meetings promoted by the North Regional Coordination
and Development Commission (CCDR-N), contributing to the elaboration of regional priorities
for the sustainable development of marine research in the North of Portugal and Galicia region
that resulted in a final report “Regional Agenda for the Sea”, published in February 2009.
Ecophysiology Group participates in NATURA-MINHO-MIÑO, a project funded by Interreg IV
POCTEP, the Transboundary territorial cooperation program between North of Portugal and
Galicia. This project aims the join valorization of natural resources in the river Minho
Hydrographical basin, the promotion of sustainable development and biodiversity and habitat
conservation (Natura 2000) in the transboundary territory of North of Portugal and Galicia. It
involves the participation of regional authorities (General Directorate of Nature Conservation in
Galicia and ICBN - Institute for Nature Conservation and Biodiversity of the Portuguese Ministry
for the Environment) assuring the effective involvement in public policy advice.
8e. Future research
1. Objectives
-
Conservation physiology is an emerging field that applies physiological tools to evaluate
the ability of animals to respond to their environment and to sustain their populations,
in response to anthropogenic pressures and global change. The group will continue to
address the questions that arise from the physiological adaptation of fishes in future
work, however, with a clear shift to using transcriptomics that can be applied to
conservation physiology. Biochemical biomarkers for HP exposure will also be
developed in pressure tolerant and intolerant species. The physiological study of the
ontogeny of ion and iron regulation will continue in lamprey. The elucidation of
mechanisms of ammonia tolerance in teleost fishes will be studied in addition to the
relationship of ammonia exposure on disease susceptibility.
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Develop further otolith finger printing (use of elemental and isotopic techniques) as
biological tracers of fish migration and for stock discrimination in the lab. Use of otolith
strontium: Calcium ratio to infer about the migratory pattern in different water salinity
environments and to track ontogenetic events. Use of macro and microstuctural studies
of otoliths to study fish age. These tools will be applied to Portuguese species of
commercial interest and/or unknown life cycles in a joint collaboration, mainly with
IPIMAR and CCMAR. Similar techniques will also be applied to age determination of
bivalve species.
-
Further elucidate shell calcification mechanisms and the identification of the physical
site (tissue) associated with the active calcium uptake from the environment. in
freshwater bivalves. Determine the impact of toxicological effects on the
biomineralization processes and immune responses in bivalves.
-
Further develop in vitro culture conditions and new conservation methods for
freshwater bivalves for the aquaculture and species environmental preservation.
-
Related to future advances will be the technological aspect of the group. This will
involve improvements and technological developments to the hydrostatic chamber
system (high pressure levels) and for remote monitoring systems.

Current funding
FCT PTDC/MAR/64016/2006, 2008-2011.
FCT PTDC/AMB/70431/2006, 2008-2011.
INTERREG IV POCTEP, NATURA-MINHO-MIÑO, 2009-2010.
EDP - Biodiversity fund, 2009-2011.

154.367 EUR, FCT PTDC/MAR/098035/2008, Ion and iron regulatory physiology in the marine
lamprey (Petromyzon marinus), a parasitic feeder.
156.840 EUR, FCT PTDC/MAR/111750/2009.
151.909 EUR, FCT PTDC/MAR/114577/2009.
155.234 EUR, FCT PTDC/CVT/110487/2009.
90.504 EUR, FCT PTDC/BIA-BEC/105266/2008, Identification of blackspot seabream (Pagellus
bogaraveo Brünnich, 1768) stocks in the NE Atlantic using otolith elemental and isotopic
analyses.
148
53.765 EUR, FCT PTDC/MAR/105150/2008, ConnectTools - Development and validation of tools
to assess connectivity in populations of marine animals.
59.268 EUR, FCT PTDC/MAR/101239/2008, Populational structure and migratory routes of the
jack mackerel, Trachurus picturatus, between Portuguese mainland and the archipelagos of
Azores,Madeira and Canary islands (JACMAC).
197.472 EUR, FCT PTDC/MAR/113065/2009, DEEPTEC - Development of technologies for the
study of deep sea organisms.
199.007 EUR, FCT PTDC/MAR/114986/2009, REPRESS - The role of hydrostatic pressure in the
physiology of fish.
113.180 EUR, FCT PTDC/MAR/113230/2009.
154.182 EUR, FCT PTDC/AGR-CFL/113334/2009.
154.506 EUR, FCT PTDC/BIA-BIC/115699/2009.
115.800 EUR, FCT PTDC/MAR/111947/2009.
149
In 2009, ECOTOX contributed to bring several projects to CIMAR and a total of 356.573,30 EUR
directly to the group: FCT and other research projects (295.858,00 EUR), pluriannual FCT funds
(13.400,00 EUR), students fees (14.300,00 EUR) and other (42.860,00 EUR). In addition, several
members participated in international meetings with traveling/stay expenses directly covered
and several projects are under evaluation (not included in the budget).
Detailed:
-
13.400,00 EUR FCT pluriannual funding
-
3.692 EUR (2009), FCT PPCDT/MAR/58244/2004, 2008-2009.
63.271 EUR (2009), FCT PTDC/MAR/71143/2006, 2008-2011. Total funding ECOTOX: 189.814
EUR.
30.128 EUR (2009), EU ERA-AMPERA 0001/2007, 2008-2011. Total funding ECOTOX: 90.384,00
EUR.
198.767 EUR FCT PTDC/AAC-AMB/102121/2008 (new project), 2010-2014.
50.040 EUR, FCT LTER/BIA-BEC/0019/2009 (new project), 2010-2014.
60.000 EUR, FCT PTDC/MAR/108369/2008 (new project), 2010-2014.
-
Others
33.015 EUR (2009) - University, industry, other sources.
150
3.000 EUR other projects.
14.300 EUR Student fees.
1. Objectives
The central objective of ECOTOX is to investigate the evolution and functioning of aquatic
ecosystems under anthropogenic and natural stress, with emphasis on estuarine and coastal
ecosystems. We are particularly interested in the assessment of long-term alterations induced
by global stressors (e.g. pollution, biological invasions and climate changes) on both biotic and
abiotic components of ecosystems, in understanding how and why these alterations may affect
their structure and functioning and how to achieve a compromise between biodiversity
conservation and sustainable development in global change scenarios. For this, we have been
working in several Portuguese ecosystems and in tropical selected areas, using 3
complementary approaches: comparison of similar ecosystems under different types and/or
levels of pollution (and other global stressors), study over time of the same ecosystem and
comparison of similar problems in temperate and tropical regions. In all cases, integrated
strategies including methods from ecotoxicology, ecology, genetics, and chemistry have been
used.
Specifically we intend to:
-
Characterize relevant ecosystems of the Portuguese coast and selected case studies in
tropical areas, including the identification of key-species and main problems (e.g.
chemical pollution, eutrophication, landscape alterations, climate changes, biological
invasions), and to study their evolution over time.
-
Assess the impact of stressors on organisms, populations and on the whole ecosystem
by using conventional approaches such as ecological and ecotoxicological monitoring,
bioaccumulation and biomagnification studies, ecological risk assessment and new
methods developed by the group (e.g. in situ and laboratory bioassays with native
species, environmental biomarkers).
-
Understand the mechanisms of action of stressors at sub-individual, individual and
population levels and how the presence of stressors can influence species relationships
(e.g. predation, competition), biotic/abiotic interactions, energy transfer and nutrient
cycling in ecosystems.
-
Contribute for the achievement of a compromise between biodiversity conservation and
sustainable development by providing tools, results and recommendations to decisionmakers supporting scientific-based environmental policies and ecosystem management.
151
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12 full papers published, 6 in press in ISI indexed journals.
-
2 book chapters, several other national publications.
-
More than 30 presentations in scientific meetings, the most part of them with published
abstracts.
-
1 new ecotoxicity lab bioassays linking behaviour with biomarkers (marine organisms).
-
Validation of 2 native species (invertebrates, reptiles) biomarkers for use in
biomonitoring studies in temperate and tropical ecosystems.
-
Cholinesterases characterization in 2 species and their responses to reference
pollutants.
-
Effects of PAHs, pesticides, metals and pharmaceuticals on several marine and
freshwater species of temperate and tropical areas, including mechanisms of toxicity
and detoxification.
-
Relationships between several biomarkers and behaviour endpoints.
-
Knowledge about contamination levels in NW Portuguese coast (Aveiro lagoon –
Spanish border), Minho, Lima, Cávado and Douro Rivers estuaries and Aveiro lagoon,
using integrated approaches including water quality variables, chemical analysis (e.g.
metals, PAHs, pesticides), bioaccumulation factors, biomarkers and condition indexes in
fish (eels; common goby).
-
Knowledge on the freshwater tidal area of Minho estuary, mainly of the macrobenthic
community, population dynamics of key-species, impact of 2005 heat wave on the
community and populations resistance and resilience.
-
Identification and characterization of the non-native invasive species introduced in the
Minho and Lima rivers. Particularly attention was focused in the non-native invasive
species Corbicula fluminea, including its impact on native species and on ecosystems.
-
Functional characterization of the community of copepods of Cávado and Minho
estuaries and knowledge about resistance to pollution and microevolution due to
pollution in selected species.
-
2 PhD Thesis and 6 MSc Thesis (1 from another other country).
-
Advanced training in Ecotoxicology and Ecology of 15 students (3 from other countries)
and 2 researchers with PhD (2 from other countries).
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8d. Productivity
1. Cairrão, E, Pereira, MJ, Morgado, F, Nogueira, AJA, Guilhermino, L, Soares, AMVM. 2009.
Phenotypic variation of Fucus ceranoides, F. spiralis and F. vesiculosus in a temperate coast (NW
Portugal). Botanical Studies 50: 205-215. IF=0.781.
2. Domingues, I, Guilhermino, L, Soares, AMVM, Nogueira, AJ, Monaghan, KA. 2009. Influence of
exposure scenario on pesticide toxicity in the midge Kiefferutus calligaster (Kieffer).
Ecotoxicology and Environmental Safety 72: 450-457. IF=2.133.
3. Gravato, C, Guilhermino. L. 2009. Effects of benzo(a)pyrene on sea bass (Dicentrarchus labrax
L.): from biomarkers to effects on growth, feeding and behavior. Human and Ecological Risk
Assessment 15: 121-137. IF=1.528.
4. Guimarães, L, Gravato, C, Santos, J, Monteiro, LS, Guilhermino, L. 2009. Yellow eel (Anguilla
anguilla) development in NW Portuguese estuaries with different contamination levels.
Ecotoxicology 18: 385-402. IF=3.507.
5. Howcroft, CF, Amorim, MJB, Gravato, C, Guilhermino, L, Soares, AMVM. 2009. Effects of
natural and chemical stressors on Echytraeus albidus: can oxidative stress parameters be used
as fast screening tools for the assessment of different stress impacts in soils? Environment
International 35: 318-324. IF=4.786.
containing anatoxin-a and of pure anatoxin-a on early of developmental stages of carp.
7. Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2009. Production of anatoxin-a by
cyanobacterial strains isolated from Portuguese fresh water systems. Ecotoxicology 18: 11101115. IF=3.507.
8. Rellán, S, Osswald, J, Saker, M, Vasconcelos, V, Gago, AM. 2009. First detection of anatoxin-a
in human and animal dietary supplements containing cyanobacteria (blue-green algae). Food
and Chemical Toxicology 47:2189-2195. IF=2.114.
Pollution 157: 2751-2758. IF=3.436.
10. Sousa, R, Gutiérrez, JL, Aldridge DC. 2009. Non-indigenous invasive bivalves as ecosystem
engineers. Biological Invasions 11, 2367-2385. IF=3.074.
11. Tim-Tim, AL, Morgado, F, Moreira, S, Rangel, R, Nogueira, AJ, Soares, AM, Guilhermino, L.
2009. Cholinesterase and glutathione S-transferase activities of three mollusk species from the
NW Portuguese coast in relation to the ‘Prestige’ oil spill. Chemosphere 77: 1465-1475.
IF=3.253.
153
12. Vieira, LR, Gravato, C, Soares, AMVM, Morgado, F, Guilhermino, L. 2009. Acute effects of
copper and mercury on the estuarine fish Pomatoschistus microps: linking biomarkers to
behaviour. Chemosphere 76: 1416-1427. IF=3.253.
In press
13. Agra, A, Guilhermino, L, Soares, A, Barata, C. (in press). Genetic costs of tolerance to metals
in Daphnia longispina populations historically exposed to a copper mine drainage.
Environmental Toxicology and Chemistry. IF=2.565.
14. Dias, S, Sousa, R, Antunes, C. (in press). Ecological quality assessment of the lower Lima
estuary. Marine Pollution Bulletin. IF=2.630.
15. Dias, S, Freitas, V, Sousa, R, Antunes, C. (in press). Factors influencing epibenthic
assemblages in the Minho estuary (NW Iberian Peninsula). Marine Pollution Bulletin. IF=2.630.
16. Gravato, C, Guimarães, L, Faria, M, Alves, A, Guilhermino, L. (in press). Comparative study
about the effects of pollution on glass and yellow eels (Anguilla anguilla) from the estuaries of
Minho, Lima and Douro Rivers (NW Portugal). Ecotoxicology and Environmental Safety.
Doi:10.1016/j.ecoenv.2009.11.009. IF=2.133.
17. Afonso, MJ, Chaminé, HI, Marques, JM, Carreira, P, Guimarães, L, Guilhermino, L, Gomes, A,
Fonseca, PE, Pires, A, Rocha, FT. 2010. Environmental issues in urban groundwater systems: a
multidisciplinary study from the Paranhos and Salgueiros spring waters, Porto (NW Portugal).
Environmental Earth Sciences, Published online November 2009, Doi: 10.1007/s12665-0090351-7.
18. Martínez-López, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, P, Guilhermino, L, GarcíaFernández, AJ. 2009. Blood delta-ALAD, lead and cadmium concentrations in spur-thighed
tortoises (Testudo graeca) from Southeastern Spain and Northern Africa. Ecotoxicology (E pub
ahead of print, 24 November). IF=3.507.
proceedings)
1. Afonso, MJ, Chaminé, HI, Gomes, A, Fonseca, PE, Marques, JM, Guimarães, L, Guilhermino, L,
Teixeira, J, Carvalho, JM, Rocha, FT. 2009. Urban hydrogeomorphology and geology of the Porto
metropolitan area (NW Portugal): a multidisciplinary approach. In: Culshaw, MG, Reeves, HJ,
Jefferson, I, Spink, TW (Eds). Engineering geology for tomorrow's cities. Engineering Geology
Special Publication, The Geological Society of London. Volume 22 (on CD-Rom insert, IAEG Paper
92, 9 pp.). ISBN: 978-1-86239-290-8; doi:10.1144/EGSP22.I
154
proceedings)
1. Oropesa, AL, Soler, F, Zalba, J, Gravato, C, Guilhermino, L. 2009. Defensas antioxidantes de
origen enzimático en cigüeña branca (Ciconia ciconia): aplicación para biomonitorización de la
contaminación por agents que originan fenómenos de estrés oxidativo. Revista de Toxicología
26: 67.
2. Morais, P, Sousa, R, Antunes, C, Solan, M. 2009. Influence of the Asian clam Corbicula
fluminea (Müller, 1774) in the Minho estuary ecosystem functioning: the effect of bioturbation.
Proceedings of the IV Iberian Symposium of the River Minho Hydrological Basin, Vila Nova de
Cerveira, 152 - 154 (in Portuguese).
3. Dias, SC, Sousa, R, Freitas, V, Vale, M, Vasconcelos, V, Antunes, C. 2009. Aestival
characterization of the River Minho estuary aquatic communities. Proceedings of the IV Iberian
Symposium of the River Minho Hydrological Basin, V. N. de Cerveira, p. 89-98 (in Portuguese).
4. Sousa R., Dias, S, Guilhermino, L, Antunes C. 2009. Faunal biodiversity in the Minho estuary:
principal threats and possible management options. Proceedings of the IV Iberian Symposium of
the River Minho Hydrological Basin, V.N. de Cerveira, p. 64 (in Portuguese).
MASTER THESES
Barroso, MFS. 2009. Efeitos ecotoxicológicos de pesticidas e factores abióticos em Daphnia
magna. Master thesis in Environmental Contamination and Toxicology, ICBAS/Faculty of
Sciences, University of Porto. Supervisor: L Guilhermino, Co-supervisor: L Guimarães.
Luís, LGS. 2009. Efeitos agudos do naftaleno no camarão Palaemon serratus: mortalidade,
resposta de biomarcadores e comportamento. Master thesis in Biology, Faculty of Sciences,
University of Porto. Supervisor: C Gravato, Co-supervisor: L Guilhermino.
Marques, MFF. 2009. Respostas aguda e crónica de Daphnia magna a cefalexina. Master thesis
in Legal Medicine, ICBAS, University of Porto. Supervisor: L Guilhermino.
Muntz, A. 2009. Linking different levels of biological organization after acute exposure of a
widely used pharmaceutical (diazepam) in Dicentrachus labrax). Master thesis in Écologie et
Catholique d’Ouest, Angers, France. Supervisor: L Guilhermino.
Oliveira, PAC. 2009. Ecotoxicological responses in wild populations of mussels (Mytilus
galloprovincialis) in relation to the exposure to selected polycyclic aromatic hydrocarbons.
Master thesis in Environmental Contamination and Toxicology, ICBAS/Faculty of Sciences,
University of Porto. Supervisor: L Guilhermino, Co-supervisor: C Gravato.
Semedo, M. 2009. Integrated biomarkers and interactive effects of copper (II) and cadmium (II)
with pyrene on antioxidant enzymes and lipid peroxidation of the mussel Mytilus
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galloprovincialis. Master thesis in Biochemistry, ICBAS/Faculty of Sciences, University of Porto.
Supervisor: L Guilhermino.
PhD THESES
Lima, IM. 2009. Ecotoxicological effects of petrochemical products on natural populations of
Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal. PhD thesis in
Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Guilhermino, Co-supervisor: Amadeu
MVM Soares (CESAM, University of Aveiro). (Thesis submitted in 2009, defense in 28 January
2010)
thesis in Biology, University of Aveiro. Supervisor: L Guilhermino, Co-supervisor: Fernando
Morgado (CESAM, University of Aveiro).
1 device for testing behavioural effects of pollution on epibenthic fish and a new toxicity
bioassay. Detailed description in: Vieira, LR, Gravato, C, Soares, AMVM, Morgado, F,
Guilhermino, L. 2009. Acute effects of copper and mercury on the estuarine fish Pomatoschistus
microps: linking biomarkers to behaviour. Chemosphere 76: 1416-1427.
-
October, Porto, Portugal. R Sousa - Member of the Organising Committee.
-
1st Iberian Congress in Cyanotoxins, University of Porto, 6-7 July, Porto, Portugal. J.
Osswald - Member of the Organising Committee.
Organization of sessions at international meetings
-
L Guilhermino - Co-organizer and co-chair of the session ET03 – Assessing the ecological
relevance of OMICS and biomarkers, SETAC Europe 19th Annual Meeting. Goteborg,
Sweden, 31 May-4 June 2009.
Seminars
-
“Children and the environment”, “Environment and childhood cancer”, “Case-report in
Paediatric environmental health”, by Dr Juan António Ortega, Chair of the Environ.
Health Committee of the Spanish Association of Paediatrics, Spain. Seminars of the
Master on Environmental Contamination and Toxicology (Course: Environment and
Public Health). ICBAS and CIIMAR, 10-13 Dec 2009.
-
“Pollution and smoking in Paediatrics” by Dr Margarida Guedes, General Hospital of
Santo António – Paediatrics Unity, Porto. Seminar of the Master on Environmental
Contamination and Toxicology (Course: Environment and Public Health). ICBAS and
CIIMAR, 11 Dec 2009.
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-
“Environment and Public Health: or when the first treads the second” by Dr. Cândida
Abreu São João Hospital, Porto. Seminar of the Master on Environmental Contamination
and Toxicology (Course: Environment and Public Health). ICBAS and CIIMAR, 11 Dec
2009.
-
“Alternative methods in toxicological science” and “Animal poisoning in Europe:
wildlife” by Prof Dr Fracesca Caloni, Faculty of Veterinary, University of Milan, Italy.
ICBAS, 12-14 May 2009.
-
“Wildlife toxicology and poisoning” and “Ilegal use of poisons” by Prof. Dr. Francisco
Soler, Faculty of Veterinary, University of Extremadura, Spain. Seminar in the scope of
the Master on Environmental Contamination and Toxicology. ICBAS & CIIMAR, 6-10 July
2009.
-
“Climate change and contaminant fluxes in Artic marine ecosystems”, “Invasive species
and climate related issues in Northern Europe”, “Ecological and physiological
perspectives of pollution-related responses in marine fish species”, “Sediment toxicity
and interactions between eutrophication, oil and contaminants”, by Prof Dr Ketil
Hylland, Biology Department, University of Oslo, Norway. Seminars in the scope of the
Master on Environmental Contamination and Toxicology and of the PhD Program on
Marine and Environmental Sciences. CIIMAR & ICBAS 18-20 March 2009.
The laboratory of Ecotoxicology provides consulting and other services to industry, including
toxicity tests and support for REACH, the new European Community regulation on chemicals and
their safe use (EC 1907/2006). In addition, the laboratory has been also performing toxicity
assays, environmental studies and consulting services regarding the management of residuals,
sediments removal and other environmental matters for national industry, local and national
Authorities (e.g. Municipalities). In addition, L Guilhermino has been participating in projects of
the Autonomous University of Campeche, Mexico, in cooperation with oil and pesticide
companies operating in the Gulf of Mexico to reduce the environmental and human health
impact of the oil extraction and transformation, and of the use of pesticides in agriculture. Also,
some members of the group participated in the meetings (restricted participation) to prepare
the international workshop “EMERCHEM - Emerging Chemicals in developing Countries” that
will bring together scientists, regulators and other involved persons and institutions from OCDE
and developing countries to identify emerging pollutants of concern in developing countries and
chemical safety. It will have the scientific leadership of SETAC and has already the support of
governments, several donor countries and several world institutions operating in these
countries. Furthermore, L Guilhermino was an invited expert in the E3 - 2nd Expert Examination
and Evaluation Workshop, promoted by an international company group operating at world
level to select appropriated strategies and methods for the exploration of Oil & Gas in the Arctic,
held in Paris from 26-27 November 2009.
L Guilhermino is Vice-President of the Society of Toxicology and Environmental Chemistry
(SETAC) - Europe, member of its Executive Council, member of World Council of SETAC and of
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several Committees of this scientific society (e.g. Science Committee, Awards Committee). She is
also member (scientific expert) of the Working Group on Biological Effects of Environmental
Contaminants of the ICES. R Sousa is a member of The European Group on Biological Invasions
Council. L Guilhermino has been evaluator of projects/grants for the International Science
Foundation. She and R Sousa have been evaluating projects for the Fondo Investigacion
Científica de Argentina. L Guilhermino is Associate Editor of the ISI journal “Biomarkers” and R
Sousa is member of the Editorial Board of “Water SA”. ECOTOX members with a PhD are regular
reviewers of scientific ISI journals (e.g. Chemosphere, Environmental Pollution, Marine Ecology
Progress Series, Environment International, Marine Pollution Bulletin, etc). The lab is a partner
of the ERA-NET project RAMOCS (EU ERA-AMPERA 0001/2007), is involved in several networks
and initiatives for international cooperation, and has intensive scientific cooperation with
research groups in Europe, Africa, America and Asia, including: BELGIUM (Univ Antwerp);
FRANCE (Univ Catholique Ouest); NORWAY (Univ Oslo, NIVA); SLOVENIA (Univ Ljublajana);
FINLAND (Finish Environmental Institute); SPAIN (CSIC Torre La Sal, CSIC Barcelona, INIA, Univ
Basque Country, Univ Coruna, Univ Extremadura, Univ Murcia, Univ Valencia, Univ Vigo); UK
(Plymouth Marine Lab, Univ Sussex, Univ Cambridge); BRAZIL (Univ Federal Baía, Univ S Paulo);
COSTA RICA (Univ Nacional); MEXICO (Univ Campeche; CIAD Mazatlán; Univ Puebla); USA (Univ
Georgia); TUNISIA (Higher Inst Agronomy Choott Mariem); INDIA (Univ Madras).
Collaborative publications:
-
Domingues, I, Guilhermino, L, Soares, AMVM, Nogueira, AJ, Monaghan, KA. 2009.
Ecotoxicol Environ Saf 72: 450-457.
-
Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2009. Ecotoxicology 18: 1110-1115.
-
Rellán, S, Osswald, J, Saker, M, Vasconcelos, V, Gago, AM. 2009. Food and Chemical
Toxicology 47:2189-2195.
-
Sousa, R, Gutiérrez, JL, Aldridge DC. 2009. Non-indigenous invasive bivalves as
ecosystem engineers. Biological Invasions 11, 2367-2385.
-
Agra, A, Guilhermino, L, Soares, A, Barata, C (in press). Environ Toxicol Chem.
-
Martínez-López, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, P, Guilhermino, L,
García-Fernández, AJ. 2009. Ecotoxicology (E-pub 24 Nov).
Several members of ECOTOX participated in meetings to establish priorities of scientific policy,
science, research and action (e.g. management, sustainable development) at global, European,
national and regional levels in several areas such as: Environment, Marine Sciences, Climate
Changes, Chemicals use in Developing Countries. In addition, L Guilhermino is member of the
Council of the North Hydrographic Region of Portugal (“Conselho da Região Hidrográfica do
Norte”) assisting and advising the Administration of the Hydrographic Region of the North of
Portugal (ARH-Norte) on the application of funds and adoption of strategies, plans and projects
related with water use and management of river basins, among other matters, contributing for
158
the development of the North Region of the country. She is member of the core group of the
project LTER/BIA-BEC/0019/2009 (total budget: 199.932,00 EUR), recently funded by FCT and
where the North and Centre Hydrographic Regions participate as end-use partners. She is also
member of the Working Group on Biological Effects of Environmental Contaminants of the
International Council for the Exploration of the Sea that develops methods and strategies to
protect the marine environment assisting several international organizations and conventions
such as OSPAR.
ECOTOX participated in the meetings promoted by the North Regional Coordination and
8e. Future research
1. Objectives
In the next future, ECOTOX will continue to investigate according the central and specific
objectives indicated in the section Objectives of this report. In the next 2-5 years, special
attention will be given to:
-
Understanding how the presence of chemical pollution drives the evolution of
populations of key estuarine species (e.g. Carcinus maenas): by studying populations
developing in estuaries with different levels of pollution and performing laboratory
assays with individual chemicals and mixtures, relevant molecular genetics alterations
will be identified; working with selected polymorphic genes, the genetic diversity and
possible differential selection pressures in populations developing in different estuaries
will be studied. (project FCT PTDC/MAR/71143/2006, 2008-2011; 1 PhD FCT grant)
-
Effects of pollution and natural stressors on ecosystem functioning will be investigated
using both in situ and laboratory ecological and ecotoxicological experiments by
assessing the effects and interactions of both chemical and natural stressors on
individuals, populations, community and abiotic component of the ecosystem. For
example, the effects on species competition (including competition between native and
NIS), predation, primary production and energy transfer will be studied using
approaches already in development. Particular attention will be given to “ecosystem
engineering” species, such as Corbicula fluminea. (3 projects: FCT PTDC/AACAMB/102121/2008, 2010-2014; FCT LTER/BIA-BEC/0019/2009, 2010-2014; FCT
PTDC/MAR/108369/2008, 2010-2014; 1 post-doc + 3 PhD grants apart from projects
budget)
-
Development of methodologies for risk assessment of oil and chemical spills will be
standardized and validated with native species, in the scope of an already funded
international project and in the continuation of work that has been performed by the
group in previous projects (e.g. CONTROL, RISKA, EROCIPS), in the Atlantic coast of
Portugal and Galicia, with particular focus on planktonic and pelagic organisms, and on
the establishment of links among “omics”, biomarkers and populational endpoints to
159
take full advantage of the new technology in understanding complex ecological and
ecotoxicological problems. (1 project: EU ERA-AMPERA 0001/2007, 2008-2011; 3 bilateral co-operation agreements; 1 post-doc + 1 PhD grant apart from the project
budget)
-
Effects of global changes: Following the important results that have been obtained in
the estuary of the Minho River about the impact of the 2005 heat wave on molluscs
assemblages and the results from the comparison between the effects of pollution in
temperate and tropical ecosystems, efforts in investigating potential effects of climate
changes will be considerably increased. We will investigate questions such as: what are
the predicted changes on the abiotic component? How these changes will affect
different populations and the relationships among them? Will these changes modify the
toxicity of individual pollutants and their toxicological interactions in complex mixtures?
How they will influence the evolution of communities under chemical stress? (1 project:
FCT PTDC/MAR/108369/2008, 2010-2014; 1 PhD grant apart from the project budget)
-
Tropical ecotoxicology: In Mexico, a project with endangered species (e.g. Crocodylus
moreletii) was recently initiated and will be continued. In Mexico (Campeche, Sinaloa,
Puebla), Brazil (Baia state) and Costa Rica, the research already going on about the
effects of pollution (oil and pesticides, mainly), climate changes, biodiversity
conservation and sustainable development will be continued. (Several bi-lateral
agreements at the University level going on; 1 project in Mexico funded by industry)

Current funding
50.767 R, FCT PTDC/AAC-AMB/102121/2008, 2010-2014.
50.040 EUR, FCT LTER/BIA-BEC/0019/2009, 2010-2014.
60.000 EUR, FCT PTDC/MAR/108369/2008, 2010-2014.
63.271 EUR per year until 2011, FCT PTDC/MAR/71143/2006, 2008-2011.
30.128 EUR per year until 2011, EU ERA-AMPERA 0001/2007, 2008-2011.
About 80.000 EUR per year (University, students fees, pluriannual, industry, other sources
including funding for travelling to Europe, America, Africa and Asia).

199.776 EUR, FCT PTDC/AAC-AMB/110331/2009, PI: L Guilhermino.
199.872 EUR, FCT PTDC/AAC-AMB/110992/2009, PI: R Sousa.
200.000 EUR, FCT PTDC/MAR/114296/2009. PI: C Gravato.
126.270 EUR, FCT PTDC/AAC-AMB/110320/2009. PI: J Osswald.
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137.779 EUR, FCT PTDC/AAAC-CLI/109863/2009. PI: L Guimarães.
188.076 EUR, FCT PTDF/AAC-CLI/098047/2008 (appeal under evaluation). PI: L Guimarães.
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Vitor Manuel Oliveira Vasconcelos
Funds spent in 2009:
-
-
National funding
2.686,86 EUR (2009), FCT CTA/46733/2004
39.734,55 EUR (2009), FCT PTDC/AMB/67075/2006, The application of quantitative real-time
PCR to studies of the abundance and toxicity of cyanobacteria in Portuguese potable and
recreational water supplies. PI: V Vasconcelos. Total funding: 140.818 EUR, 2008-2010.
41.728,32 EUR (2009), FCT PTDC/MAR/68106/2006 (2009), The modulation of retinoic acid
signalling pathways by environmental pollutants in teleosts. PI: M Santos (Environmental
Toxicology Group), Researcher: A Antunes. Total funding: 188.980 EUR, 2008-2011.
19.698,46 EUR (2009), FCT PTDC/BIA-BDE/70982/2006, Phenogenetic drift in evolution: insights
into the genetic basis of vertebrate developmental genes. PI: A Antunes. Total funding: 148.000
EUR, 2008-2010.
-
European funding
57.623,33 EUR (2009), INTERREG IVB Atlantic Area, ATLANTOX - Advanced Tests about New
Toxins appeared in the Atlantic Area. PI: V Vasconcelos. Total funding: 138.033,43 EUR, 20092010.
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1. Objectives
LEGE - Laboratory of Ecotoxicology, Genomics and Evolution is a laboratory of CIIMAR and
CIMAR-LA dedicated to the study of toxic organisms and its natural toxins diversity, including
production mechanisms and control of their occurrence in a ecotoxicological and evolutionary
genomic/proteomic perspective. It is also a major aim, to study the toxin-tolerance levels among
varied organisms (from humans, to other animals and plants), which requires the molecular
characterization of relevant cellular mechanisms, including detoxification, development and
genetic disease onset. Specific objectives:
-
Ecotoxicological study of cyanobacteria and other potentially toxic organisms in marine,
brackish and freshwaters. Occurrence of the main species and of the main toxins, and
production of bioactive compounds with potential pharmacological interest.
-
Ecotoxicological assays regarding the effects of neurotoxins and hepatotoxins in
different organisms. Use of behavioral and histopathological alterations as endpoints.
Studies on the bioaccumulation of toxins and research of possible toxin vectors. Human
health risk assessment of toxin occurrence in drinking, recreational waters. Impact of
contamination of toxins in water used for agriculture.
-
Use of molecular and computational genomics/proteomics tools to characterize
systemic functional behaviors of the cell and the organism (from humans, to other
animals and plants). This includes understanding the interaction and adaptive evolution
of cellular protein-coding genes operating in toxin production, detoxification,
development, and genetic disease onset in various organisms.
-
Use of molecular genomics for assessing the biogeography and phylogenetic
relationships of toxic organisms (from cyanobacterias to metazoans) and non-toxic
organisms with varied resistance to the effects of natural toxins.
The development of new analytical techniques, namely HPLC and Capilary Electrophoresis for
the detection of BMMA in marine and freshwaters was applied to water matrices. We also
developed and validated an HPLC-FL method for anatoxin-a in fish tissues and a HPLC-DAD for
microcystins using methanol as mobile phase due to scarcity and expensive price of acetonitrile.
Early warning methods for the detection and quantification of bacteria and cyanobacteria and
cyanotoxins based on molecular approaches (RT-PCR) were developed and applied monitor
these organisms in different water systems. Cylindrospermopsis raciborskii and Microcystis
aeruginosa, and their toxins, cylindrospermopsin (CYN) and microcystins (MC) were detected
and quantified in Portugal using qRT-PCR.
Low densities of cyanobacteria have allelopathic effects in green algae and we isolated and
structurally characterized new allelopathic substances - portoamides - produced by a freshwater
Oscillatoria.
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Worldwide strains of the toxic C. raciborskii were evaluated and the phylogenetic analysis
showed that the C. raciborskii strains grouped into three well-supported distinct clusters: (I)
European, (II) Africa/America and (III) Asia/Australia. The obtained results suggest that the
recent invasion of C. raciborskii in Portuguese and other European temperate environments
could have had its origin in the Asian and/or Australian continents.
MC profiles were studied in different environments showing a high diversity of variants either in
strains isolated from a Portuguese reservoir or from a Moroccan Atlas river. Anatoxin-a, a
neurotoxin from cyanobacteria, was found for the first time in strains isolated from Portuguese
freshwaters as also from dietary supplements containing cyanobacteria.
Effects of MC on germination and growth of several plants showed that there is a species
specific reaction to MC and that bioaccumulation may occur. Acute and chronic effects of
anatoxin-a and MC were assessed in fish larvae showing that ecologically relevant
concentrations of these toxins may have an impact in fish populations. Exposure of bivalves to
MC cause not only significant changes in detoxication enzymes such as GST, as also a differential
protein expression.
Phylogenetic analyses assessed the evolution and functional diversification of the cytosolic GSTs.
Protection against oxidative stress seems to be the major driver of positive selection in
mammalian cGSTs, explaining the overall expansion pattern of this subfamily. Moreover, the
catalytic promiscuity of cGSTs has certainly facilitated the process of neofunctionalization and
duplicate gene retention.
PhosphoProtein Phosphatases (PPP) enzymes involved in key cellular processes such as
morphogenesis, synaptic transmission and apoptosis are the target of multiple naturallyoccurring toxins, namely MC. The phylogeny of PPPs among metazoans and the molecular
interaction of varied toxins with the PPPs, highlighted (1) which specific structural differences
within the similarly folded catalytic core of the PPPs explain their diverse sensitivities to toxin
inhibition, and (2) which structural features presented by the various toxins account for the
differential inhibitory potency towards each PPP.
The phylogenetic relationships and adaptive evolution of important metazoan gene products
involved in key cellular processes and often disrupted by environmental contaminants were
assessed. We characterized the gene/protein evolution of the Retinoid X Receptor (RXR), a
nuclear receptor highly relevant the embryonic development of the vertebrate embryos; and
the matrix extracellular phosphoglycoprotein (MEPE) that belongs to a family of small integrinbinding ligand N-linked glycoproteins with an important role in skeleton development,
particularly in the mineralization process.
Software tools to perform evolutionary analyses on gene/protein sequences were developed:
(1) IMPACT - a new GUI that estimates evolutionary rates on protein sequences by assessing
changes in biochemical constraints, phylogeny and protein structure; and (2) TI2BioP
(Topological Indices to BioPolymers) - an alignment-free approach that allows the calculation of
the spectral moments as simple topological indices (TIs) to seek Quantitative Sequence-Function
Relationships (QSFR) models, which we successfully applied to the study of bacteriocins,
proteinaceous toxins produced by both gram-negative and gram-positive bacteria.
164
During 2009, we published a total of 26 papers in SCI journals, presented 12 oral
communications and 40 posters in national and international conferences.
8d. Productivity
1. Osswald, J, Carvalho, AP, Claro, J, Vasconcelos, VM. 2009. Acute effects of cyanobacteria
anatoxin-a on carp (Cyprinus Carpio L.) early stages of development. Ecotoxicology and
Environmental Safety 72: 473-478. IF=2.133.
2. Martins, JC, Vasconcelos, VM. 2009. Microcystin distribution and dynamics in aquatic
organisms - a review. Journal of Toxicology and Environmental Health - Part B. Critical reviews.
12: 1-18. IF= 3.617.
3. Saqrane, S, El Ghazali, I, Oudra, B, Bouarabab, L, Dekayir, S, Mandi, L, Ouazzani, N,
Vasconcelos, VM. 2009. Reversibility of in vivo chlorophyll fluorescence in Lemna gibba exposed
to microcystins. Toxicon 53(1): 9-14. IF=2.128.
4. El Ghazali, I, Saqrane, S, Carvalho, AP, Youness, O, Oudra, B, del Campo, FF, Vasconcelos, V.
aeruginosa natural bloom extract. International Journal of Molecular Sciences 10(1): 133-146.
IF=1.387.
5. Oudra, B, Dadi-El Andaloussi, M, Vasconcelos, VM. 2009. Identification and quantification of
microcystins from a Nostoc bloom occurring in Oukaïmeden River (High-Atlas Mountains of
Marrakesh, Morocco). Environmental Monitoring and Assessment 149: 437-444. IF=1.356.
6. Martins, JC, Leão, P, Vasconcelos, VM. 2009. Differential protein expression in Corbicula
fluminea upon exposure to a Microcystis aeruginosa toxic strain. Toxicon. 53: 409-416. IF=2.128.
7. Fernandes, S, Welker, M, Vasconcelos, V. 2009. Changes in the GST activity of the mussel
Mytilus galloprovincialis during exposure and depuration of microcystins. Journal of
Experimental Zoology - Part A 311: 226-230. IF=1.444.
IF=3.124.
9. Martins, J, Saker, M, Moreira, C, Welker, M, Fastner, J, Vasconcelos, V. 2009. Peptides
produced by strains of the cyanobacterium Microcystis aeruginosa isolated from Portuguese
water supplies. Applied Microbiology and Biotechnology 82(5): 951-961. IF=2.896.
10. Pereira, S, Saker, M, Vale, M, Vasconcelos, VM. 2009. Comparison of sensitivity of grasses
(Lolium perenne L. and Festuca rubra L.) and lettuce (Lactuca sativa L.) exposed to water
contaminated with microcystins. Bulletin of Environmental Contamination and Toxicology 83:
81-84. IF=0.992.
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11. Sabour, B, Sbiyyaa, B, Loudiki, M, Oudra, B, Belkoura, M, Vasconcelos, V. 2009. Effect of light
and temperature on growth of two toxic Cyanobacteria forming-blooms in Oued Mellah Lake
(Morocco). Chemistry and Ecology 25(4): 277-289. IF=0.634.
green microalgae at low cell densities. European Journal of Phycology 44(3): 347-355. IF=2.270.
13. Rellan, S, Osswald, J, Saker, M, Gago, A, Vasconcelos VM. 2009. First detection of anatoxin-a
in human and animal dietary supplements containing cyanobacteria. Food and Chemical
Toxicology 47(9): 2189-2195. IF=2.114.
14. Sabour, B, Loudiki, M, Vasconcelos, V. 2009. Growth responses of Microcystis ichthyoblabe
Kützing and Anabaena aphanizomenoides Forti (Blue-green algae) under different nitrogen and
phosphorus conditions. Chemistry and Ecology 25(5): 337-344. IF= 0.634.
15. Almeida, AM, Campos, A, van Harten, S, Cardoso, LA, Varela Coelho, A. 2009. Establishment
of a proteomic reference map for the gastrocnemius muscle in the rabbit (Oryctolagus
cuniculus). Research in Veterinary Science 87: 196-199. IF=1.345.
16. Agüero-Chapin, G, Varona, J, de la Riva, GA, Antunes, A, González-Villa, T, Uriarte, E,
González-Díaz, H. 2009. Alignment-free prediction of Polygalacturonases with pseudo-folding
topological indices: experimental isolation from coffea arabica and prediction of a new
sequence. Journal of Proteome Research 8(4): 2122-2128. IF=5.132.
17. Osswald, J, Rellan, S, Gago, A, Vasconcelos, VM. 2009. Production of anatoxin-a by
cyanobacteria strains isolated from Portuguese fresh waters. Ecotoxicology 18: 1110-1115.
IF=3.507.
18. Costa, ML, Santos, MCR., Carrapiço, F, Pereira, AL. 2009. Azolla-Anabaena's behaviour in
urban wastewater and artificial media - Influence of combined nitrogen. Water Research 43(15):
3743-3750. IF=4.355.
19. Cianca, RCC, Barbosa, RD, Faro, LRF, Adan, LV, Gago-Martínez, A, Pallares, MA. 2009.
Differential changes of neuroactive amino acids in samples obtained from discrete rat brain
regions after systemic administration of saxitoxin. Neurochemistry International 54(5-6): 308313. IF=3.541.
20. Leão, P, Vasconcelos, MTSD, Vasconcelos, VM. 2009. Allelopathy in freshwater
cyanobacteria. Critical Reviews in Microbiology 35(4): 271-282. IF=3.207.
21. Campos, A, da Costa, G, Coelho, Varela A, Fevereiro. P. 2009. Identification of bacterial
protein markers and enolase as a plant response protein in the infection of Olea europaea
subsp. europaea by Pseudomonas savastanoi pv. Savastanoi. European Journal of Plant
Pathology 125: 603-616. IF=1.931.
22. Saker, M, Moreira, C, Martins, J, Neilan, B, Vasconcelos, V. 2009. DNA profiling of complex
bacterial populations: toxic cyanobacterial blooms. Applied Microbiology and Biotechnology 85:
237-252. IF=2.896.
166
23. Regueiras, A, Saker, M, Vasconcelos, V. 2009. Use of PCR for the detection of pathogenic
bacteria and cyanobacteria in water samples from different urban water sources (Porto,
Portugal). Fresenius Environmental Bulletin 18(12): 2365-2365. IF=0.531.
24. Pereira, AL, Carrapiço, F. 2009. Culture of Azolla filiculoides in artificial media. Plant
Biosystems, 143: 431-434. IF=0.744.
25. Pereira, AL, Figueiredo, AC, Barroso, JG, Pedro, LG, Carrapiço, F. 2009. Volatile compounds
from the symbiotic system Azolla filiculoides-Anabaena azollae-bacteria. Plant Biosystems 143:
268-274. IF=0.744.
26. Amorim, A, Veloso, V, Frazão, B, Alves de Matos, AP. 2009. TEM identification of Pseudonitzschia species from Lisbon Bay (NE Atlantic). Microscopy and Microanalysis, 15(supp3): 9-10.
IF=3.035.
proceedings)
1. Moreira, C, Lopes, VR, Vasconcelos, V. 2009. Diversidade e análise da toxicidade de
cianobactérias no ecossistema estuarino do rio Minho. IV Simpósio Ibérico sobre a Bacia
Hidrográfica do Rio Minho, pp. 147-151, ISBN: 978-989-96062-0-3.
proceedings)
MASTER THESES
Encarnação, AM. 2009. Uso de métodos químicos na avaliação da toxicidade de cianobactérias.
Master thesis in Sciences and Environmental Technologies, Faculty of Sciences, University of
Porto, Supervisor: V Vasconcelos.
Freitas, M. 2009. Monitorização de cianobactérias e cianotoxinas nas lagoas de Mira e Vela
comparando métodos moleculares, imunológicos e volumes de amostragem. Master thesis in
Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of
Porto. Supervisor: V Vasconcelos.
Gouveia, A. 2009. Conservação e educação ambiental no contexto de um Centro de
Monitorização e Interpretação Ambiental (CMIA de Vila do Conde). Master thesis in Biology,
Faculty of Sciences, University of Porto. Supervisor: V Vasconcelos.
Laverco, P. 2009. Análise biológica de biofilmes em sistemas de transporte de águas de
University of Porto. Supervisor: V Vasconcelos, Co-supervisor: J Poças Martins (Faculty of
Engineering, University of Porto).
167
Maldonado, E. 2009. Development of a graphical user interface for a software used to assess
adaptive evolution in protein-coding genes. Master thesis in Computer Sciences, Faculty of
Sciences, University of Porto. Supervisor: A Antunes, Co-supervisor: V Vasconcelos.
Morais, J. 2009. Avaliação do risco de ocorrência de cianotoxinas nos lagos do Parque da Cidade,
Porto. Master thesis in Biology and Management of Water Quality, Faculty of Sciences,
University of Porto. Supervisor: V Vasconcelos.
Regueiras, A. 2009. Comparação de métodos de identificação e quantificação de cianobactérias
e suas toxinas na albufeira do Torrão (Rio Tâmega). Master thesis in Contamination and
Environmental Toxicology, Faculty of Sciences/ICBAS, University of Porto. Supervisor: V
Vasconcelos.
Saraiva, F. 2009. Desenvolvimento de ferramentas didático-educativas na área da divulgação
ambiental no centro de Monitorização e Interpretação Ambiental de Vila do Conde. Master
thesis in Sciences and Environmental Technologies, Faculty of Sciences, University of Porto,
Supervisor: V Vasconcelos.
-
1st Iberian Congress in Cyanotoxins, 6-7 July 2009, Porto, Portugal. V Vasconcelos - Chair
of the Organizing and Scientific Committees. http://www.cimar.org/cic2009/
-
X Reunião Ibérica de Fitoplâncton Tóxico e Biotoxinas, IPIMAR, May 2009, Lisbon,
Portugal. V Vasconcelos - Member of the Scientific Committee.
LEGE (V Vasconcelos) has a contract with NETIRA Ambiente, Tratamento de Águas, in order to
evaluate ecotoxicologically two new products developed to be used in the water treatment of
natural and artificial systems.
Industry contract research with Hovione FarmaCiencia S.A. on survey of some pharmaceutics
effects on freshwater cyanobacteria, does good manufacturing practice (GMP) during
pharmaceutics manufacture influence the biological response?
V Vasconcelos: Stephan Pflugmacher, - IGB, Berlin, Germany, Stein-Oven Doskeland, U. Bergen,
Norway, Brahim Oudra, U.Cadi Ayyad, Marrakesh, Morocco; Ana Gago, U.Vigo, Spain; Amel
Jenhani, INAT, Tunis- Tunisia, Jordi Molgo, Lab. of Molecular & Cellular neurobiology, CNRS,
Paris, France, Gustavo Montejano, Lab. of Phycology, National U. of Mexico, Mexico; Luis
Botana, U. Santiago de Compostela, Lugo, Spain, Ana Maria Camean, Faculty of Pharmacy, U.
Seville, Spain.
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National Contact (Portugal) of CYANONET- Global Network for the Hazard Management of
Cyanobacterial Blooms and Toxins in Water Resources, of the International Hydrology
Programme of UNESCO (2005-)(V Vasconcelos)
Member of the Task Force - Marine and Freshwaters Toxins – of the Association of Official
Analytical Chemists- AOAC International (2007-)(V Vasconcelos)
A Antunes: Stephen J O’Brien, Warren E Johnson, Shu-Jin Luo, Jennifer Troyer, Jill PeconSlaterry, Melody E Roelke, Lab. of Genomic Diversity, NCI, NIH, MD, USA; Gustavo de la Riva,
Sylvester Comprehensive Cancer Center, U. of Miami, Florida, USA; Sue VandeWoude and Sam
Franklin, Colorado State U., CO, USA; Craig Packer, U. of Minnesota, Minnesota, USA; Laurence
Frank, U. of California Berkeley, CA, USA; Katherine C Prager, U. of California Davis, CA, USA;
David Wildt & Mitch Bush, Smithsonian’s National Zoological Park, Front Royal, VA, USA; KuoChen Chou, Gordon Life Science I., San Diego, CA, USA; Eugenio Uriarte, ITESS, Guanajuato,
Mexico; Humberto Gonzalez-Diaz, Florencio M. Ubeira, U. Santiago de Compostela, Spain;
Graham Hemson, Wildlife Conservation Research Unit, Tubney, Oxon, UK; Christiaan
Winterbach, Hanlie Winterbach, Tau Consultants, Maun, Botswana; Kathy A. Alexander,
Department of Wildlife and National Parks, Kasane, Botswana; Philip Stander, Ministry of
Environment & Tourism, Windhoek, Namibia; Ludwig Siefert, Makerere U., Kampala, Uganda;
Margaret Driciru, Uganda Wildlife Authority, Kampala, Uganda; Paul J. Funston, Tshwane U. of
Technology, Pretoria, South Africa; Gus Mills, U. of Pretoria, South Africa; Paolo Martelli Ocean
Park, Aberdeen, Hong Kong; Olga Uphyrkina, I. of Biology & Soil Sciences, Vladivostok, Russia.
A Campos: Mireya Santos & Josep Maria Torné, Center of Research in Agrogenomics (CRAG)CSIC-IRTA-UAB, Barcelona, Spain.
LEGE has been responsible for the analysis of water and counseling to many public health
departments in what concerns the environmental and human health risk management
associated with toxic cyanobacteria and other phytoplankton.
LEGE has been working in a national project coordinated by INAG (Portuguese Water Institute,
Ministry of Environment) in the Water Framework Directive of Costal Waters. Responsible for
the phytoplankton analysis of northern estuaries.
V Vasconcelos joined the team of Quaternaire in order to elaborate the Management Plan for
the Alto Rabagão Reservoir (2008-2009) being responsible for the Waster Quality Chapter.
V Vasconcelos is also consultant with INAG in order to coordinate a national intercalibration
exercise for phytoplankton analysis in freshwaters.
V Vasconcelos, as the scientific Coordinator of CMIA Vila do Conde (Interpretation and
Monitoring Center of Vila do Conde) has directed the study of the physico-chemical and
microbiological quality of the beaches of Vila do Conde area. A Report on the Water Quality of
those beaches during 2008 was published and distributed to Public health Authorities,
Municipality of Vila do Conde and CIMAR.
169
Contracts with National authorities:
-
Avaliação do estado ecológico das massas de água costeiras e de transição adjacentes e
do potencial ecológico das massas de água fortemente modificadas (Ecological status of
coastal and transitional waters of the Portuguese coast). In the Framework of POVT/
Eixo III - Combate à Erosão Costeira. Funded by QREN, 2009-2010 (V asconcelos).
-
Ensaio laboratorial de fitoplâncton de águas doces. Coordenação do exercício
interlaboratorial nacional (National interlaboratory coordination exercice). Funded by
INAG, 2009-2010 (V Vasconcelos).
Moreover, LEGE participated in the meetings promoted by the North Regional Coordination and
8e. Future research
1. Objectives
Development of early warning methods for the detection and quantification of cyanobacteria
and cyanotoxins using molecular approaches (RT PCR) and ecotoxicological approaches. Search
for new bioactive compounds including new toxins from marine environments using marine
invertebrates as sources. Occurrence, transmission and risk analysis of tetrodotoxin. Risk
analysis of the BMMA in marine and freshwater environments including development of new
analytical techniques, HPLC and Capilary Electrophoresis. Dynamics of cyanotoxins
bioaccumulation and its effects on organism metabolism. Study of the action of known and
unknown toxins from cyanobacteria using a proteomic approach. Study of action of known and
unknown toxins from cyanobacteria using a proteomic approach.
Evolutionary genomics/proteomics studies of cellular protein-coding genes operating in venom
production, detoxification, development, immune system, and genetic disease onset; and the
inference of the comparative genomic principles from various organisms (from marine
microorganisms to mammals) that participate in these processes. Such patterns of genomic
variation across organisms are of critical value in interpreting the structural dynamics and the
functional diversifications that occurred in organism’s genomes over millions of years of
evolution. Such knowledge also provides reliable insight for biodiversity studies of various
species, from marine microorganisms to mammals.

Current funding
140.818 EUR, FCT PTDC/AMB/67075/2006, 2008-2010.
170
188.980 EUR, FCT PTDC/MAR/68106/2006, 2008-2011.
148.000 EUR, FCT PTDC/BIA-BDE/70982/2006, 2008-2010.
138.033,43 EUR, INTERREG IVB Atlantic Area, ATLANTOX, 2009-2010.
209.969 EUR, INTERREG IV B Atlantic Area, ShareBiotech, Sharing life science infrastructures and
skills to benefit the Atlantic area biotechnology sector, 2010-2012. PI: A Antunes.
95.513,76 EUR, INTERREG IV B Atlantic Area, PHARMATLANTIC, Knowledge transfer network for
prevention of mental diseases and cancer in the Atlantic Area. 2010-2012. PI: V Vasconcelos.
199.997 EUR, FCT PTDC/AAC-AMB/104983/2008, Evolutionary genomic analyses of toxic
cyanobacteria and study of the molecular mechanisms for detoxification of cyanotoxins across
metazoan species. 2010-2012. PI: A Antunes.
126.706 EUR, FCT PTDC/MAR/099642/2008, Phylogeny and chemodiversity of marine sponge
symbiotic Cyanobacteria. 2010-2012. PI: V Vasconcelos.
87.024 EUR, FCT PTDC/MAR/102258/2008, Secondary metabolites and nitrogen fixation of
benthic marine Cyanobacteria: how different from freshwater relatives? 2010-2012. PI: V
Vasconcelos.
124.570 EUR, FCT PTDC/MAR/102638/2008, Marine cyanobacteria isolated from the Portuguese
coast as a source of bioactive compounds: cytotoxicity against human tumor cells and normal
human cells. 2010-2012. PI: V Vasconcelos.
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Maria Clara Semedo da Silva Costa
The funding sources of Environmental Technologies group of CCMAR in 2009 were:
-
1.761,81 EUR FCT Pluriannual funding.
-
2.062,50 EUR, partial budget given by FCT to the University of Algarve for the PhD
scholarship of Mónica Sofia Furtado Martins, SFRH/BD/29677/2006.
1. Objectives
The central aim of the Environmental Technologies group is to contribute to the better
knowledge and development of sustainable biotechnologies based on the use of bacteria for the
decontamination of metal contaminated waters or effluents.
In the framework of Mónica Martins PhD
The objectives of the third year of her PhD entitled “Bio-removal of toxic metals by metal
resistant anaerobic bacteria: molecular characterization and performance studies” are
described:
-
Molecular characterization of uranium resistant bacterial consortia.
In previous studies, two anaerobic bacterial consortia resistant and with ability for
2uranium(VI) removal were selected among several environmental samples. One of
these consortia (A) was from an uncontaminated site, Monchique thermal place, and
the other one (U) was from the uranium mine site of Urgeiriça, both in Portugal. The
molecular identification of the two consortia was aimed.
172
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Mechanism of U(VI) bio-removal
To understand the mechanism of uranium(VI) removal by the two anaerobic bacterial
consortia previously characterised was a central objective of her work.
-
Dynamics of a bacterial community in a bioremediation process for acid mine drainage
(AMD) treatment using wine wastes as carbon source
The studies carried out in the first years of the PhD showed that winery wastes can be
used as carbon sources for sulphate-reducing bacteria (SRB) for the bioremediation of
AMD, with great economic advantages. Thus, the dynamics of a bacterial community
exposed to those wastes during the bioremediation process was considered.
-
Screening of SRB resistant to Al(III)
Considering the lake of information about the use of bacteria for the treatment of
aluminium contaminated waters and effluents, the search for SRB eventually resistant
to Al(III) and preferably with ability for Al3+ and sulphate removal was included as a
topic of her PhD work.
In the framework of Maria Fernandes postdoc
Biological processes based on SRB are considered the only low cost and environmentally
sustainable technology for AMD decontamination. However, this technology still faces several
shortcomings. One of them is the low sulphate reduction rate of the bacterial consortia, which
leads to low bioremediation rates. The main inhibitory factors of sulphate reduction are the
toxicity of the dissolved heavy metals and the low pH of the environment. Thus, the objective of
this postdoc project, initiated in 2009, is to isolate SRB from several natural metal-rich and metal
contaminated environments, considering their eventual resistance to unusually high metal
concentrations. The isolated bacterial consortia were evaluated for their adequacy for AMD
bioremediation and the most promising inocula were physiologically and genetically
characterized.
In the framework of João Costa PhD
The general objectives of his PhD work entitled “Bio-synthesis of nanosized semiconductors
using mine wastes as materials sources” and the specific objectives for the first year of his
scholarship are following described:
The main goal of this work is the synthesis of metal sulphide nanoparticles using by-products of
a sulphate-reducing biological process, previously developed by this group during ECOTEC
project (POCI/PPCDT/AMB/58512/2004) for AMD treatment. The production of nanomaterials
directly during the bioremediation processes will be also attempted.
For 2009, the main goals were to determine, in batch systems, the optimal conditions for the
synthesis of metal sulphide nanoparticles, using solutions containing H2S biologically produced.
The influence of media composition, namely presence/absence of suspended bacterial cells,
sulphate and carbon sources concentration, as well as the use of support materials, were
investigated. The evaluation of these parameters will allow subsequently the use of the optimal
173
conditions for the production of metal sulphide nanomaterials as a result of the AMD
bioremediation process.
-
Molecular characterization of uranium resistant bacterial consortia
Phylogenetic analysis showed the composition of two bacterial communities (A and U) resistant
and with ability for U(VI) removal: Community A were mainly composed by bacteria closely
related to Sporotalea and by bacteria related to members of Rhodocyclaceae family, while
community U was mainly composed by bacteria affiliated to Clostridium and by bacteria related
to members of Rhodocyclaceae family.
-
Mechanism of U(VI) bio-removal
The lack of metal removal by extracellular products and heat-killed cells suggested that only
viable cells can be responsible for uranium (VI) removal from the solution. XRD analysis of the
precipitate formed during the bio-removal experiments showed that it was mainly composed by
uranium oxide as U307. This result, together with the fact that U(VI) was only removed from
solution in the presence of live cells, suggests a mechanism of enzymatic reduction, where U(VI)
is converted to insoluble U(IV). TEM images showed the presence of dense precipitates mainly
within the periplasmic region of cells of both consortia, which gives indication about the
reductase location.
-
Dynamics of the bacterial community involved in a bioremediation process for AMD
treatment using wine wastes as carbon source
TGGE analysis suggests that the structure of the bacterial community was affected by the type
of carbon source (lactate, ethanol or wine wastes) used in a bioremediation process used for
AMD treatment. Higher bacterial variability was observed in the reactor fed with wine wastes
comparatively to that fed with ethanol. The phylogenetic analysis of TGGE band sequences, that
will allow the identification of the dominant populations, are being performed.
-
Screening of SRB resistant to Al(III)
Among several bacterial communities obtained from various environmental samples, only one
consortium from a sludge sample from the wetland of Urgeiriça mine showed resistance to
Al(III) and capacity for sulphate and Al3+ removal.
The sulphate reduction performance and the tolerance to the main metals present in AMD (Fe,
Zn and Cu) were tested with SRB consortia from several environmental samples. As a result of
this survey, a highly metal tolerant consortium also very fast in sulphate reduction was isolated
from one Iceland hot spring sample. This consortium is tolerant to and grows in a combination
of 750 mg/L of iron, 170 mg/L of zinc and 80 mg/L of copper. These metal concentrations are
higher than those usually found in Portuguese AMD. After 28 days of exposure, 3500 mg/L
174
sulphate was reduced, precipitating the dissolved metals (Fe, Cu and Zn) as insoluble metal
sulphides. Iron was precipitated by 100%, zinc by 96% and copper by 97%. The metal tolerance
and metal removal performance of the described consortium is higher than those described in
literature (Martins et al., 2009. J. Haz. Mat. 166, 706-713). This highly tolerant consortium was
analyzed by cloning and sequencing assays based on the 16S rRNA gene and is composed of two
genera - Clostridium sp. and Desulfovibrio sp. The results of the cloning assays were confirmed
by DGGE analysis of the consortium.
Additionally, a SRB consortium was isolated for the first time from the highly acidic and metal
contaminated soil (pH of 3.6) of the S. Domingos mine region. This is a very unusual finding,
since SRB are not supposed to thrive at pH lower that 6. This natural consortium displayed high
rates of both sulphate and metal removal in the sediments. Moreover, when incubated at pH 4,
sulphate reduction in the sediments was detected once. However, when bacteria are detached
from the original sediments, all bacterial resistance against metals and pH ceases. Studies are
being undertaken to establish the possible correlation between the soil matrix and the
resistance to low pH and metal tolerance.
Several SRB cultures previously grown in rich Postgate media have been attempted to grow in
the simple Sani medium. Although, the advantages of having simple media, containing less
compounds that could eventually interfere in the formation of the metal sulphide nanoparticles,
they have proven to have a quite limited sulphate removal capacity, making them, to this point,
inadequate for the nano-synthesis process. Thus, so far, the potential effect of the components
of the media, such as the carbon source and their degradation products, on the synthesis of
metal sulphide nanoparticles has not yet been established.
8d. Productivity
1. Costa, MC, Santos, ES, Barros, RJ, Pires, C, Martins, M. 2009. Wine wastes as carbon source
for biological treatment of acid mine drainage. Chemosphere 44: 477-480. IF=3.253.
2. Martins, M, Faleiro, ML, Barros, RJ, Veríssimo, AR, Costa, MC. 2009. Biological sulphate
reduction using food industry wastes as carbon sources. Biodegradation 20(4):559-567.
IF=1.873.
3. Martins, M, Faleiro, ML, Barros, RJ, Veríssimo, AR, Barreiros, MA, Costa, MC. 2009.
Characterization and activity studies of highly heavy metal resistant sulphate-reducing bacteria.
Journal of Hazardous Materials 166: 706-713. IF=4.144.
4. Barros, RJ, Jesus, C, Martins, M, Costa, MC. 2009. Marble stone processing powder residue as
chemical adjuvant for the biologic treatment of acid mine. Process Biochemistry 44: 477-480.
IF=2.444.
175
proceedings)
proceedings)
Costa, MC, Martins, M, Jesus, C, Barros, R. 2009. Uso de lama de mármore para pré-tratamento
químico de efluentes ácidos. Portuguese Patent DMP/01/2009/204286.
Research collaboration with Dr. Haitham Sghaier from National Centre for Nuclear Science and
Technology (CNSTN) in Tunisia was established during 2009. In that context, a Joint research
bilateral agreement proposal between Portugal and Tunisia, as well as an international project
were already prepared and submitted (see pending funding).
As a result of our collaboration with Prof. Jose Miguel Nieto, from the Geology Department of
Huelva University, the exchange of several graduate training students between both groups has
been undertaken.
176
8e. Future research
1. Objectives
The crescent demanding and consequent economical importance of platinum group metals
(PGM) greatly justify the search for bacteria resistant and with ability for their removal and
recovery.
The potentialities of newly synthesised organic compounds for the liquid-liquid extraction of
PGM, is also a subject that this group aims to pursue.
Due to the high cost of the PGM, the two topics of research above mentioned are highly
dependent on financial support.
Although the importance of metal removal from aqueous media, the bio-removal processes
usually generates solid materials that need to be disposed. Thus, in order to find out possible
applications to those wastes it is aimed in the future research to investigate if the metal
precipitates biologically produced are nanosized, which can perspective unusual properties for
new applications.
In view of the optimization and study of the bioremediation potential of bacteria resistant to
metals and/or to ionizing radiations for use at nonradioactive and/or radioactive waste sites, the
isolation of bacteria from phosphogypsum piles (disposed at Quimiparque, Barreiro, Portugal)
will be attempted. The isolation of bacteria from phosphogypsum from other locations, such as
Huelva in Spain and eventually Sfax in Tunisia, will be also attempted. The chemical,
mineralogical and bacterial composition of the different mineral sources will be investigated in
order to establish possible correlations.
-
To deeply explore the enzymes responsible for metals bioreduction and their potential
for useful applications is a natural development of her research work. These studies
include the identification of those enzymes and the investigation of their catalytic
activity.
-
Studies will be undertaken to address the role that each bacteria (Clostridium sp. and
Desulfovibrio sp.) presented in the consortium is playing in the bioremediation process.
-
In following experiments, the bioremediation potential of that consortium will be tested
at lower pH. At pH 2-3 usually found in AMD, the reduced metal ions permeate cell
membranes more easily. Therefore, we will test various possibilities of increasing the
acid tolerance of the bacterial consortium: addition of biosurfactants, gradual
adaptation, and addition of a support matrix. In these studies, the viability and
membrane potential of the cells carrying out the bioremediation will be assessed by
fluorescence microscopy and image analysis which will allow the monitoring of the
population at the single cell level. These results will provide valuable information for the
improvement of the bioremediation process.
177
-
For the inoculum isolated from S. Domingos mine studies will be first carried out in
order to reproduce with reliability the observations made in the first assay. Secondly the
role of the soil in the tolerance to metals and pH will be investigated.
-
The bio-synthesis of metal sulphide nanoparticles and nanocomposites still be tested in
batch systems in order to better clarify the parameters that can affect the synthesis of
these nanomaterials (eg. pH, redox potential, presence and absence of bacterial cells,
type and concentration of carbon sources and their degradation products). These data
will be essential to establish the best conditions to be further used when the synthesis
occurs during or as a consequence of the AMD bioremediation process.
-
Morphological and structural characterization of the nanomaterials will be performed
using suitable techniques, such as X-ray powder diffraction (XRD), X-ray photoelectron
spectroscopy (XPS), scanning electron and transmission electron microscopy (SEM and
TEM) associated to an energy dispersive system (EDS).
-
Studies of the correlation between the preparation methods and the exhibited
properties attributed to the nanosized dimension and/or morphology will be performed.

Pending funding
101.257 EUR, FCT PTDC/AAC-AMB/110337/2009, BIONANOMINE - Bio-synthesis of nanosized
semiconductors using mine wastes as material sources and environmentally friendly
applications. PI: MC Costa.
53.246 EUR, FCT PTDC/QUI-QUI/109970/2009, Separation of precious and rare metals from
chloride complex solutions by liquid-liquid extraction, potential environmental and recycling
applications. Principal contractor: Fundação Fac Ciências, PI: Ana Paula Paiva, Participating
institution: CCMar/CIMAR.
11.040 EUR, FCT PTDC/BIA-MIC/115306/2009, ACIDYEAST - An integrated approach involving
phylogenomics to unveil metal resistance mechanisms and its evolution in acidophilic yeasts.
Principal contractor: Fundação Fac Ciências, Participating institution: CCMar/CIMAR.
5.160 EUR, FCT PTDC/EQU-EQU/110974/2009, BROMA DBP BIOBLAST - Bromate removal and
control of disinfection by-products and pollutants using biologic activated carbon filters.
Principal contractor: Univ Algarve, Participating institution: CCMar/CIMAR.
FCT bilateral agreements, Joint Research Project Proposal Tunisia - Portugal 2009, Study of
microbial metal-removal to bioremediate non radioactive and radioactive wastes. Funding for 4
missions of the Environmental Technologies group to the National Center for Nuclear Sciences
and Technologies (CNSTN) in Tunisia, 2010-2011.
235.000 EUR, Mediterranean Sea Basin Joint Operational Program, Project “Optimization of the
bioremediation potential of bacteria resistant to metals and/or to ionizing radiations for use at
178
nonradioactive and/or radioactive waste sites in Mediterranean countries”. Joint Managing
Authority: Autonomous Region of Sardinia, Cross-Border Cooperation within the European
Neighbourhood and Partnership Instrument (ENPI).
179
Maria Armanda Reis Henriques
Funding spent in 2009:
-
24.500,00 EUR, FCT Pluriannual funding
-
National funding
2.497,17 EUR, FCT PPCDT/MAR/60895/2004, Effects of complex mixtures of antagonistic
endocrine disrupting chemicals in two fish species with different life histories. PI at CIIMAR: M
Santos.
45.943,62 EUR, FCT PTDC/MAR/68106/2006, The modulations of retinoic acid signaling
pathways by environmental pollutants in teleosts. PI: M Santos.
64.500,00 EUR, FCT PTDC/MAR/105199/2008, The invertebrate repertoire of nuclear receptors:
evolutionary and endocrine disruption insights. Responsible partner at LETOX: M Santos,
03/2010-02/2013.
-
European Funding
47.787,75 EUR, EU 2008/1-061, INTERREG IV Atlantic Area, ARCOPOL - The Atlantic Regions’
COastal POLlution Response. Total funding: 265.120 EUR, 01/2009-12/2011. Partner Scientific
Leader: MA Henriques.
180
1. Objectives
The research of the Environmental Toxicology Group (LETOX) in 2009 focused in several areas: In
the scope of endocrine disruption (ED) we pursed several complementary aims. In the aquatic
environment endocrine disrupting chemicals (EDCs) do not exist alone. Therefore, single
ecotoxicological evaluations may not reflect the multitude of antagonistic or synergistic stimulus
that wildlife animals may face. Hence, under controlled laboratory conditions we aimed at
evaluating the effects and the molecular and biochemical mechanisms of disruption of fish and
marine invertebrate’s exposure to EDCs. Furthermore in the scope of a FCT project, we have
started the study of the mechanisms of disruption of ECDs acting through the retinoic acid
signaling pathways. The new research area concerning pharmaceuticals in the aquatic
environment started. Among the quantity of pharmaceuticals sold, psychopharmaca comprise a
mayor group in terms of sales – particularly in Portugal. Since they specifically target
neurotransmitters in the brain, severe impact on vital functions such as the endocrine regulation
has to be assumed at minute concentrations. In an environmental monitoring study, prevailing
psychopharmaceuticals in a hot spot estuary (Douro) are identified and subsequently considered
as model substances to reveal their impact on the main hormone systems (reproduction,
osmoregulation, growth and development) in fish. Assessing the neuroendocrine disruption will
not just provide sophisticated data for environmental risk assessment but allow for new insights
on pituitary neuroendocrinology and the interlinkage of hormone systems, contributing to a
better understanding of fish endocrinology. In parallel, we continued our research on the
mechanisms of detoxification of organic contaminants including a new topic of research on
efflux membrane transporters in fish, the multidrug resistance pump P-glycoprotein (Pgp) and
the multridrug resistance-associated proteins (MRPs) involved in the multixenobiotic resistance
(MXR). We intended to establish the impact of fish feeds on aquaculture production systems on
the sea food quality and safety and the contribution to the control of flesh quality and oxidative
stability, together with health benefits for consumer. In the scope of the European project
ARCOPOL we started the work with the objective to establish methodology for environmental
damage assessment, based on hazard evaluation of the most relevant HNS.
In the scope of ED, we have continued an innovative approach which aims the understanding of
the effects of antagonistic EDCs under complex mixtures, both in invertebrates and fish. In a
step further to understand the effects of one of the main estrogenic chemicals, the synthetic
estrogen ethinylestradiol, we have demonstrated that chronic low levels (below 0.5 ng/L) induce
a significant impact in embryo development in the second generations at concentrations below
those inducing an increase in vitellogenin gene expression. This is very relevant from a risk
assessment point of view. We have also initiated a project which aims at addressing the
toxicological impact of ECDs in the retinoic acid signaling pathways of fish. We have also isolated
several nuclear receptors in invertebrates, and, at the moment, we are testing the functionality
of such signaling pathways, and whether they are prone to disruption by several environmental
chemicals. In the scope of the development and validation of biomarkers for environmental
monitoring, we have focused our attention in the use of the blenny Lipophrys pholis in
estrogenic pollution monitoring under field and laboratory conditions.particularly towards
Polycyclic Aromatic Hidrocarbons (PAHs) pollution. Our data clearly demonstrate that this
181
species has a high potential for pollution monitoring in the northwestern European marine
ecosystems. From Human health point of view, we have evaluated the risk of consumption of
seafood in relation to organotin levels. Our results indicate that while the general consumer may
be exposed to low levels of these ECDs, certain group may exceed the tolerable average residue
levels (TARL), and this is a cause of concern. We established the impact of fish feeds and water
on aquaculture production of two species sea bass and white sea bream by the evaluation the
presence of persistent organic pollutants (POPs) and the more representative metals (Cd, Hg, Pb
and As). We have now cloned and sequenced several ABC transporter genes in two important
model species, the European seabass (Dicentrarchus labrax) and nile tilapia (Oreochromis
niloticus), as well as gene expression of the mention genes in different conditions of exposure to
PAH in Nile Tilapia. Moreover, MXR activity assays and phase I biotransformation enzymes
(EROD) in seabass primary cultured hepatocytes have been proven to be fully functional and this
new in vitro system can become a useful tool to screen pollutants effects, at the detoxification
level, in a marine species. In the ARCOPOL project a bibliographic review of HNSs spill was
carried out, using some criteria as the sea traffic extension, alerts and incidents, physical
behaviour of the substances released, chemical toxicity to marine organisms, a list containing 12
HNS was selected. A first approach in monitoring HNS contamination, based on the biological
responses at individual level by measuring physiological markers and biomarkers of
exposure/effect were developed in the crustacean artemia franciscana and the amphipode
gamarus exposed to different concentrations of xylene the first HNS of the list to be studied.
8d. Productivity
Comparative Biochemistry and Physiology C 149(1): 58-64. IF=2.582.
aurata) to in vivo exposure to pyrene and fluorine. Journal of Experimental Marine Biology and
Ecology 372(1-2): 49-57. IF=2.116.
aurata) to in vivo exposure to a mix of selected PAHS. Ecotoxicology and Environmental Safety
72(4): 1296-1302. IF=2.133.
4. Reis-Henriques, MA, Ferreira, M, Coimbra, AM, D'Silva, C, Costa, J, Shailaja, MS. 2009.
Phenanthrene and nitrite effects on juvenile sea bass, Dicentrarchus labrax, using hepatic
biotransformation enzymes, biliary fluorescence and micronuclei as biomarkers. Ciencias
Marinas 35: 15-27. IF=0.655.
5. Santos, MM, Enes, P, Reis-Henriques, MA, Kuballa, J, Castro, LF, Vieira, MN. 2009. Organotin
levels in seafood from Portuguese markets and the risk for consumers. Chemosphere 75(5): 661666. IF=3.253.
182
7. Trubiroha, A, Wuertz, S, Frank, SN, Sures, B, Kloas, W. 2009. Expression of gonadotropin
subunits in roach (Rutilus rutilus, Cyprinidae) infected with plerocercoids of the tapeworm Ligula
intestinalis (Cestoda: Bothriocephalidea). International Journal for Parasitology 39: 1465-1473.
IF=3.819.
proceedings)
proceedings)
-
2009, Porto, Portugal. MA Henriques and M Santos - Members of the Organising
Committee. http://www.ciimar.up.pt/7aiec/index.html
Spain
-
Institut de Ciences del Mar - CSIC, Dr. Montserrat Sóle; the collaboration deals with the
effects of oil pollution in fish and invertebrates.
-
Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR).
183
Germany
-
Federal Institute of Hydrology (Koblenz), Dr. Thomas Ternes, Dr. Guido Fink; Chemical
analysis of pharmaceutical residues in the aquatic environment.
-
Institute of Freshwater Ecology and Inland Fisheries (Berlin), Professor Dr. Werner Kloas,
Dr. Ilka Lutz; Expression analysis of single cells (Laseer dissection microscope, qPCR), two
ongoing projects (“Parasites as endocrine disruptors”, “Reproductive physiology of
Sander”).
Italy
-
ENEA Centro Ricerche Casaccia, Dr. Roberto Morabito and Dr. Paolo Massanisso; we
seek to understand the effects of pollutants with endocrine disrupting properties, in
sexual development and reproduction of fish.
-
Dipartimento di Scienze Ambientali, Università Ca’ Foscari Venezia.
Tunisia
-
University of Bizerte, Laboratory of Environment Bio-monitoring, Department of
Biology; the collaboration focus on the effects of EDCs in molluscs.
Japan
-
Osaka University, Department of Toxicology, Prof. Junichi Nishikawa; Together with this
group, we try to better understand the effects of EDCs in invertebrates, through
modulation of nuclear receptors. This collaboration has already resulted in one
publication in a reputable international journal.
Croatia
-
Rudjer Boskovic Institute (Zagreb), Laboratory for Molecular Ecotoxicology, Dr. Tvrtko
Smital; purpose to increase the knowledge on the detoxification mechanisms in aquatic
species, by studying phase 0 and III.
India
-
National Institute of Oceanography, Dona Paula, Goa; To evaluate the impact of the
more representative organic pollutants in some species of fish living in estuaries and
coastal zones.
France
-
184
Conseil Régional d’Aquaculture, Conseil Régional de Bretagne, Conseil Régional des Pays
de la Loire, Conseil Régional de Poitou Charentis.
United Kingdom
-
Department of Biological Sciences, Brunel University, Centre for Toxicology, The School
of Pharmacy, University of London, Devon Country Council, Dorset Country Council,
Pembrokeshire County Council, Environmental and Heritage Services, Devon Wildlife
Trust.
LETOX participated in the meetings promoted by the North Regional Coordination and
LETOX participates in ARCOPOL - The Atlantic Regions Coastal Pollution Response, a project
funded by Interreg IV Atlantic Area, the transnational territorial cooperation program between
regions of the Atlantic Area. This project aims to improve the preparedness, response and
mitigation capabilities of local responders to accidental coastal pollution specifically against oil,
hazardous and noxious substances and inert spills. It involves the participation of regional and
national authorities (National Maritime Authority of the Ministry of National Defence and Port
Authority in Portugal) assuring the effective involvement in public policy advice.
8e. Future research
1. Objectives
-
The research on the MXR mechanisms will be continued focusing on evaluating
interaction of pollutants with the efflux transporters, and in general the detoxifications
mechanism in aquatic species. Some chemicals can compromise this defence
mechanism by inhibiting the transporters in such a way that chemicals normally
excluded from the cells can now exert their toxic effects, sensitising the cell - hence the
term chemosensitization. In vitro models, as primary cultured hepatocytes from marine
and fresh water species, will be used to address the chemosensitization problem, in the
presence of pollutant mixtures. Moreover, in the near future we will also assess the role
of these important efflux transporters in chemically induced neoplasia in the model
species zebrafish.
-
In the field of ED, we will continue the research involving the study of chemical mixtures
with antagonistic effects. We will focus in changes at different levels of biological
organization, i.e., from changes in gene expression up to alterations on population
dynamics. We have shown the importance of the modulation of the retinoic acid
signalling pathways in the development of ED in gastropods. We will continue this line
of research including the impact in fish. One of the innovative aspects is the study of
185
disruption mechanisms in phylogenetically distinct groups thus taking into account an
evolutionary perspective.
-
Since psychopharmaceuticals (PP) target the secretion of the principal pituitary
hormones in humans, it has to be expected that ecotoxicological impact affects several
endocrine systems. Due to the relevance of salinity as major ecological factor in the
estuaries and coastal zone, PP-induced neuroendocrine disruption is addressed in an
experimental approach with regard to osmotic challenge with special focus on the main
hormones involved in freshwater saltwater adaptation. Parameters evaluated to
characterise the adverse effects incorporate molecular/physiologic (gene expression,
plasma hormones), the organ/tissue-specific (gross morphology, histology) as well as
the organisms (growth, reproduction, osmotic homeostasis) level. Impact on four
endocrine systems (adrenal, growth, reproduction and osmoregulation) will be
addressed and characterized as chronic change in gene expression (real-time RT-PCR for
gene expression analysis) of the pituitary hormones. The resulting impact on the
regulated organs (e.g. ovary) is evaluated by plasma hormone concentration and gene
expression of selected markers (e.g. vitellogenin mRNA expression in response to
estradiol). In addition, simple behaviour tests are applied to access swimming
performance and courtship behaviour.
-
In the scope of ARCOPOL project work will be developed in two main areas:
methodology for environmental damage assessment (review of impacts of past oil and
HNS incidents on marine ecosystems; review of ecological risk assessment
methodologies for oil and HNS pollution incidents and standardize monitoring
procedures for the assessment of environmental damage and restoration after spill oil
or HNS. And the second area establishing methodologies for economic damage
assessment of marine marketable resources (identify major gaps in the build up of
economic damage information of marine marketable resources for the support of
claims; decontamination kinetics in marine marketable resources after oil and HNS
spills; standardize procedures for management of contaminated marine marketable
resources).

Current funding
71.000 EUR, FCT PTDC/MAR/68106/2006, The modulations of retinoic acid signalling pathways
by environmental pollutants in teleosts. 09/2008-08/2011. PI: M Santos.
265.120 EUR, INTERREG IV, Atlantic Area, ARCOPOL - The Atlantic Regions’ COastal POLlution
Response. 01/2009-12/2011. Partner Scientific Leader: MA Henriques.

Pending funding
186.875 EUR, FCT PTDC/MAR/098791/2008, The role of ABC transporter proteins in chemically
indiced haepatic cancer in zebrafish (Danio rerio). 2010-2012. PI: M Ferreira.
186
199.960 EUR, FCT PTDC/BIA-BEC/105622/2008, The evolution of retinoid and thyroid hormone
signalling pathways, and their modulation by endocrine disruption chemicals. 2010-2012. PI: M
Santos.
156.030 EUR, FCT PTDC/MAR/100837/2008, Psychopharmaceuticals as neuro-endocrine
disruptors in fish. 2010-2012. PI: S Würtz.
187
Evolution, Development and Gene Expression (EDGE)
Evolution, Development and Gene Expression (EDGE)
Maria Leonor Quintais Cancela Fonseca
-
18.763 EUR FCT Pluriannual funding
-
60.000 EUR, PTDC/MAR/71685/2006, HYDRAA, 04/2008-03/2011.
83.450 EUR, PTDC/MAR/70855/2006, FATTYBONE, 08/2008-07/2011.
161.115 EUR, PTDC/CVT/72083/2006, ISOPERK, 08/2007-07/2010.
60.000 EUR, PTDC/MAR/70858/2006, TEXBREAM, 06/2008-05/2011.
10.000 EUR, PTDC/MAR/64533/2006, CRYOSPERM, 08/2008-07/2011.
New funding :
130.000 EUR, PTDC/SAU-ESA/101186/2008, BIOMARK, 01/2010-12/2012.
170.267 EUR, PTDC/MAR/105313/2008, FISHCELL, 01/2010-12/2012.
195.107 EUR, PTDC/MAR/105152/2008, SPECIALK, 02/2010-01/2013.
80.583 EUR, PTDC/CVT/102481/2008, ULTRAFISH, 02/2010-01/2013.
-
Prizes
50.000 EUR, Programa na Fronteira das Ciências da Vida, Prize awarded by the Calouste
Gulbenkian Foundation, 2009-2012.
50.000 EUR, CERATONIA, Prize awarded by the Caixa Geral de Depósitos/UALG, 2008-2009.
188
-
International funding
16.000 EUR, EU/FP6, SEACASE-2005-SSP5A.
266.824 EUR (CCMAR), EU/FP7, ASSEMBLE-2008-227799.
99.000 EUR (CCMAR), EU-FP7, PROSPAWN-SME-2008-1.
50.000 EUR, POCTEP, ECOAQUA/0251/5/E.
-
Collaborative actions
COST ACTION FA0801, LARVANET (no budget).
-
Industrial contracts
20.000 EUR, NOVOZYMES (Denmark).
1.127 EUR, TECHNOPHAGE (Portugal).
1. Objectives
I. Moolecular determinants of tissue mineralization
-
Develop fish & amphibians in vitro systems to analyse mineralization-related pathways,
gene expression & regulation, and test mineralogenic effects of pharmaceutical drugs &
environmental pollutants.
-
Identify orthologs of mammalian bone Gla-proteins and other bone- & cartilage-related
proteins in fish, study their spatio-temporal expression & transcriptional regulation to
bring insight into their function & evolution.
-
Identify human orthologs of novel genes identified in fish, identify their function,
regulation and relevance to human pathologies.
-
Determine microRNAs involved in fish skeletogenesis and in vitro mineralization and
identify target gene(s).
-
Identify genes, proteins & regulatory pathways involved in normal and abnormal
skeletal development & mineralization using high-throughput genomic and proteomic
tools.
-
Implement zebrafish as a model system to study human pathologies involving abnormal
calcification and identify marker genes and molecular mechanisms involved.
189
-
Establish purification methodologies and use proteomic approaches to identify new
proteins involved in calcium metabolism. Establish relevance for human health and
disease.
-
Development of embryonic stem (ES) cells from fish for the production of transgenic
animals and to achieve differentiation into various mesenchyme derived lineages.
-
Explore the mechanisms of action of nutrients on skeletal ontogenesis and development
of anomalies. Identification of regulated genes / proteins and molecular pathways
involved in osteogenesis.
II. Perkinsus-bivalve interactions
-
Determine Perkinsus infection prevalence in bivalves along Portuguese coast by
performing an extended & continued survey. Identify species permissive/nonpermissive to the Perkinsus parasite.
-
Identify environmental parameters affecting Perkinsus survival/proliferation/infection.
-
Develop molecular diagnostic tools to evaluate bivalve infection by Perkinsus.
-
Develop integrated high-throughput genomic tools to identify Perkinsus genes &
metabolic pathways by functional genomics.
-
Analyse Perkinsus-clam interactions using subtractive hybridization & differential display
approaches to unveil genes & metabolic pathways involved in parasite infection.
III. Population genetics
-
Development of molecular tools suitable for paternity/sexing and genotyping wild
populations for conservation & management purposes, including Portuguese
populations of Bonelli’s eagle and otters, and economically relevant marine crustaceans
and fish, as well as for implementation to aquaculture species of interest.
IV. Assessment of fish welfare and quality using proteomics
-
Identification of protein biomarkers related to fish welfare.
-
Study of nutritional effects and harvesting conditions in fish quality.
I. Molecular determinants of tissue mineralization
-
Development of bone-derived cell lines from a variety of marine & freshwater fish and
amphibian (Include now 5 cell lines from seabream, 2 from zebrafish, 2 from salmon, 1
from Xenopus laevis).
-
On going update of our previously developed fish cell line database (FICELdb).
190
-
Purification & cloning of OC& MGP in a variety of fish (including an ancestral OC in
sturgeon). Evidence for 2 isoforms in fish vs 1 in mammals.
-
Characterization of the evolutionary relationship of Gla proteins and identification of
domains/residues essential for function.
-
Identification of a new Vitamin K dependent protein, Gla Rich Protein, present in fish as
well as in humans and characterization of its expression in human tissues in health and
disease.
-
Proposed GRP as a new marker for ectopic calcification in humans to be pursued in the
scope of a new biotech company (GENOGLA) created by members of the EDGE group to
explore this possibility.
-
Cloning of mineralization-related gene promoters from fish/amphibians, analysis of
promoter activity and identification of transcription factor binding sites.
-
Identification of signalling pathways regulating in vitro mineralization of fish bonederived cell lines.
-
Proteomic analysis of oyster nacre & identification of osteoinductive proteins.
-
Implementation of zebrafish rearing and microinjection facilities.
-
Development of new techniques for DNA delivery into embryos (lipid-based and lasermediated delivery) & bone-derived cells.
-
Establishment of a comparative proteomics platform for fish protein identification.
-
Identification of proteins related to bone deformities in fish by 2DE techniques.
-
Developed seabream blastula-derived stem cell culture capable of producing a
mineralized matrix in vitro, a feature never achieved before in a fish stem cell line.
-
Characterization of fish skeletal deformities by immuno-histomorphological approaches.
II. Perkinsus-bivalve interactions
-
Development of a clonal culture of Perkinsus olseni (PO) allowed in vitro testing of drugs
(e.g. antimalarial) and ex vivo clam infection to identify environmental conditions
affecting parasite proliferation/survival.
-
Development of a PCR-ELISA-based assay to determine Perkinsus levels & species.
-
Extended survey of PO prevalence along Portuguese coast. Infection linked to water
temperature/salinity/pollution, iron-dependent, and inhibited by glyphosate &
pyrimethamine.
-
Identification through functional genomics of target metabolic pathways (e.g. shikimate,
folate) to control Perkinsus infection in hatcheries.
191
III. Populations genetics
Development & optimization of
-
Molecular markers (microsatellites) for Bonelli’s eagle towards conservation studies,
Norway lobster for management stock studies and Senegalese sole for offspring
assignment.
-
Techniques to preserve/extract DNA from faecal samples suitable to estimate effective.
-
Population size (offered as external service), to preserve and extract DNA from samples
with low amount of cells/eggs from Norway lobster and Senegalese sole.
-
Paternity/sexing and genetic diversity analyses to determine differential contribution
from brooders in specific populations (management and aquaculture).
8d. Productivity
1. Ascenso, RMT, Leite, RB, Afonso, R, Cancela, ML. 2009. Expression pattern of Perkinsus olseni
genes in response to bivalves with different susceptibility to Perkinsosis. Journal of Fish Diseases
32(7): 633-636. IF=1.697.
2. Conceicao, LEC, Aragao, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2009. Recent
advances in nutrition of fish larval. Revista Brasileira de Zootecnia 38: 26-35. IF=0.557.
Aquaculture 288(3-4): 264-272. IF=1.925.
4. Gavaia PJ, Domingues S, Engrola S, Drake P, Sarasquete C, Dinis MT, Cancela ML. 2009.
senegalensis, Kaup 1858): evaluation in larval and postlarval stages. Aquaculture Research
40(14): 1585-1593. IF=1.099.
5. Laizé, V, Tiago, DM, Aureliano, M, Cancela, ML. 2009. New insights into mineralogenic effects
of vanadate. Cellular and Molecular Life Sciences 66(24): 3831-3836. IF=6.090.
derived plastids. BMC Evolutionary Biology 9: 253. IF=4.294.
7. Roberto, VP, Gavaia, PJ, Viegas, CSB, Ortiz Delgado, JB, Sarasquete, C, Cancela, ML. 2009.
Molecular cloning of matrix Gla protein from turbot (Scophthalmus maximus): gene expression
192
analysis and identification of sites of protein accumulation. Aquaculture 294(3-4): 202-211.
IF=1.925.
8. Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML,
Simes, DC. 2009. Gla-rich protein (GRP) is a novel vitamin K dependent protein present in serum
and accumulated at sites of pathological calcifications. American Journal of Pathology 175(6):
2288-2298. IF=5.673.
In press
9. Dâmaso-Rodrigues, ML, Pousão-Ferreira, P, Ribeiro, L, Coutinho, J, Bandarra, NM, Gavaia, PJ,
Narcis,o L, Morais, S (in press) Lack of essential fatty acids in live feed during larval and post
larval rearing: effect on the performance of juvenile Solea senegalensis. Aquaculture
International, Doi:10.1007/s10499-009-9296-9. IF=0.753.
proceedings)
Published proceedings from international meetings
1. Marques, CL, Laizé, V, Cancela, ML. 2009. An in vitro fish system to unravel bone-related
mechanisms of BMP2. Proceedings of the 34th FEBS Congress, 4-9 July, Prague, Czech Republic.
FEBS Journal 276: 111.
2. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2009. Bone-related role of the LIM-only protein
FHL2 in fish. Proceedings of the 36th ECTS Meeting, 23-27 May, Vienna, Austria. Bone 44: S311S312.
3. Rodrigues, PM, Silva, TS, Cordeiro, O, Conceição, LEC. 2009. Proteomic characterization of
reared white seabream skeletal deformities using two-dimensional gel electrophoresis: a
comparative study. Proceedings of the 3rd EuPA Congress, Clinical Proteomics P04-08.
proceedings)
Books
1. Dias, E, Cancela, ML, Fonseca, L, Beja, P, Dentinho, T. 2009. Gestão da Água: Teses em Gestão
e Conservação da Natureza Vol II. Principia, Scientific and University Publications, Cascais,
Portugal.
Articles in national journals
2. Marques, CL, Rafael, MS, Tiago, DM, Cancela, ML, Laizé, V. 2009. Desenvolvimento de
sistemas celulares de peixe adequados ao estudo da mineralização in vitro. Canal BQ 6: 15-20.
193
MASTER THESES
Santos, RPP. 2009. Identificação de efeitos de xenobioticos in vivo no desenvolvimento do osso
em peixe zebra (Danio rerio). Master thesis in Marine Biology, University of Algarve. Supervisors:
ML Cancela, PJ Gavaia and AB Brito.
Silva, JC. 2009. Efeito da dieta e da inanição na performance larvar e no desenvolvimento de
peixe-zebra (Danio rerio): caso específico do desenvolvimento esquelético. Master thesis in
Marine Biology, University of Algarve. Supervisors: ML Cancela, PJ Gavaia and AB Brito.
EDGE has been involved in applied research that resulted in the submission of 3 international
patents in collaboration with Novozymes (Denmark)
1. 11577.000-EP, filed on 06/05/2009, Inventors: ML Cancela, V Laize, R Leite, S Danielsen
ML Cancela has also been involved in the deposition of a national patent (104425-INPI, filed on
06/03/2009) and 4 national trademarks (nº439369-72) for LAB-IT name and logo.
Organization of conferences in 2009:
-
April, Tavira, Portugal. ML Cancela, PJ Gavaia, A Bensimon-Brito, R Leite, V Laizé Organizing Committee. Approx. 100 participants from 12 countries.
-
Seminários do Mar e Ambiente, 8 May, Faro, Portugal. Coordinated by ML Cancela.
-
CCMAR Course on 2D-DIGE, October 13-15, Faro, Portugal. Organized by PM Rodrigues.
Organization of conferences and workshops programmed for 2010:
-
practice, 29 January 2010, Faro, Portugal. A Alves, ML Cancela et al. - Organizing
Committee.
NOVOZYME SA (Denmark): Research collaboration aiming at the identification of genes with
biotechnological applications. Construction of cDNA libraries for Illumina sequencing and
posterior assemblage and gene identification.
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TECHNOPHAGE SA (Portugal): Research collaboration aiming at the discovery of new molecules
with therapeutic potential for human bone-related diseases. Proof-of-concept experiments.
Research
-
FP7 I3-infrastructure ASSEMBLE (10 MEUR, 8 institutes, approved). Improvement of
provision of marine animal cell cultures/lines and tools for functional genomics.
-
COST ACTION FA0801 Larvanet.
-
Project evaluations (e.g. EC, Treaty of Windsor, INRA, CRSNG, French National Research
Agency (ANR), Dutch Research Council-NWO, INTAS), journal editor (J Bone Mineral
Research, IF 6.004) and referees (15 journals).
-
Collaborative actions with groups in Spain, UK, France, USA (FCT, CRUP, GRICES, British
Council, FLAD).
-
Oral communications + plenary lectures at international conferences (~4).
Training
-
Training of 7 MSc & 9 PhD students, 4 post-docs and international students.
-
Teaching of undergraduate ERASMUS/SOCRATES students in ongoing University
degrees.
-
Hosting visiting researchers: Norway, Belgium, Germany, Spain, France, UK.
-
Coordination of a master course on Biology of Bone and Cartilage (Master degree in
Biomedical Sciences, UALG). Teaching involved lecturers from France, Germany and
Belgium.
-
Participation as “Examinateur” of one PhD thesis in Biological Sciences, University of
Sciences and Technology of Lille, Institut of Biology/Lille. France (11 December 2009).
Research dissemination
-
9 international publications, 27 communications in international meetings, organization
of 2 international symposiums/workshops.
Invited Lectures
-
Cancela, ML. Improving the provision of cultures of marine protists and cell lines of
marine animals, ASSEMBLE Meeting, March 2009, Roscoff, France.
-
Cancela, ML. Fish as model organism to uncover new Gla proteins of interest for human
biology, IAFSB, April 2009, Tavira, Portugal.
195
Public awareness
-
LAB-IT®-The Itinerant Laboratory in Molecular Genetics. Project aiming to bring
molecular biology and genetics to high schools. A collaboration between UAlg, EDGE
laboratory, Direction of the Education Board in Algarve, the centers for Training High
school teachers and various high schools in the Algarve region
Cancela ML (since January 2009) - President of the Board of the Assembly of the Portuguese
Biochemistry Society, a non-profit, nationally recognized organization promoting the
development and dissemination of biochemistry in Portugal. Of particular relevance has been
the collaboration in the organization of a special interest meeting (Clinical Biochemistry, Faro
Jan 2010) and promoting Biochemistry and Molecular Biology education in local high schools
through seminars, training of high school teachers and implementation of laboratory classes.
Cancela ML - National representative of the Portuguese Society of Biochemistry in the
International Union of Biochemistry and Molecular Biology (IUBMB) and the Pan American
Biochemistry and Molecular Biology Association (PABMB).
8e. Future research
1. Objectives
I. Molecular determinants of mineralization/calcium mobilization (ML Cancela, V Laizé, DM
Tiago, P Gavaia, D Simes, N Conceição, PM Rodrigues)
Genes & regulatory pathways involved in skeletogenesis, environmental adaptations, tissue
regeneration (ML Cancela, V Laizé, DM Tiago, P Gavaia, N Conceição)
-
Using zebrafish & seabream as model systems, integrated approach, functional
genomics, proteomics, bioinformatics, in-house developed tools including new cell lines,
DNA microarrays, microRNA arrays, antibodies, cDNA and genomic libraries.
Collaboration with 18 groups from 9 countries.
Family of Gla-containing proteins: new members, evolution, function in heath & disease (ML
Cancela, D Simes)
-
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Development of immuno-based diagnostic assays for new antigen detection in biological
samples relevant for human health (Cancela, Simes and Viegas 2008 - patent pending;
Viegas et al, Am J Pathology, 2009). Collaboration with USA as well as Canadian, French
& Portuguese groups working in hospitals.
New osteocalcin isoforms (V Laizé, D Simes)
-
Being first to identify existence of osteocalcin isoforms (Laizé et al, 2006), our goal is to
complete this study by identifying their function & evolution. Collaboration with USA.
Gene expression & skeletal deformities (P Gavaia, D Simes, PM Rodrigues)
-
Development of diagnostic tools suitable to detect skeletal malformations in fish
through genomic, proteomic, microarray, histological-based tools (Roberto et al,
submitted). Collaboration with 7 groups (including CCMAR-Aquaculture group) from 6
countries.
Proteins from oyster nacre with osteoinductive function (D Simes)
-
Following our identification of nacre proteins as in vitro mineralization promoters their
molecular structure, regulation & function will be analysed.
New cell lines from model fish species (V Laizé, ML Cancela, V Parameswaran, N Conceição)
-
Following development of FIRST fish bone-derived cell lines (seabream; Pombinho et al.
2004), we now seek at developing additional lines from other fish (e.g. zebrafish, sole,
salmon) requiring in vitro homologous systems (FP7 project ASSEMBLE, funded).
Protein biomarkers involved in fish welfare and quality (PM Rodrigues)
II. Unveiling Perkinsus spp (PE) metabolic pathways suitable as therapeutic targets to limit
infection and for biotechnological applications (ML Cancela, R Leite)
-
Study of PE intracellular machinery, PE as model for toxicological/metabolic/genomic
studies, expression/silencing of PE genes involved in virulence, PE potential as
unicellular factory to synthesize bio-molecules of economical interest. Collaboration
with 7 groups from 5 countries.
III. Accessing wild population’s variability, sex, parental gamete contribution, genetic diversity (S
Mira, ML Cancela)

Goals based on previous achievements including: i) Identification of multiple paternity in
Norway lobster and correlation with degree of stock exploitation, ii) Differential
contributions of fish brood stock for progeny and implementation of selective breeding
programs, and iii) Survey/sexing/assessment of Bonelli’s eagle and otter populations for
management of wild populations. Collaboration in LIFE & FCT projects with 6 groups
from 4 countries.
IV. Organization of an international workshop

IV SPB Clinical Biochemistry Workshop (2010) Cancela ML, Simes DC, Leite R are
members of the organizing committee. Workshop to be held on January 29, 2010, Faro,
Portugal.
197

Symposium on Gene Expression and Disease at the National Congress of the Portuguese
Society of Biochemistry (2010) Cancela ML. Symposium to be held on December 15-17,
Porto, Portugal.

Pending funding
-
135.875 EUR, PTDC/MAR/102269/2008, DEEPBONE - Bone biology of deep-sea teleost fish .
PTDC/BIA-BCM/115609/2009, MEFTARGET - Identificação de genes alvo do factor de transcrição
MEF2C através de uma abordagem de genomica funcional e o seu papel na osteoporose.
194.244 EUR, PTDC/MAR/114444/2009, PERK-MSEQ - Trancriptomics para prospecção da
biodiversidade nativa entre hospedeiro e parasita: posterior identificação de novas moléculas
com potencial biotecnológico.
195.159 EUR, PTDC/SAU-BID/114254/2009, ZEFUSION - O peixe zebra como modelo biomédico
para estudo do processo de fusão das vértebras.
75.425 EUR, PTDC/MAR/110574/2009, DUSK - Novel methodologies for the assessment of
Dusky grouper growth potential: integrating molecular and metabolic approaches.
99.757 EUR (CCMAR), FCT PTDC/MAR/110547/2009, EPISOLE - Epigenetic regulation of
development and growth in Senegalese sole (Solea senegalensis).
181.848 EUR, PTDC/BIA-BCM/114918/2009, IPSCELL - In vitro reprogramming of zebrafish skin
derived cells into pluripotent ES-cell-like state to generate chimera fish.
178.316 EUR, PTDC/BIA-BCM/112811/2009, BONEMIR - MicroRNAs regulating vertebrate bone
development and mineralization.
194.957 EUR, PTDC/MAR/112992/2009, AQUATOX - Assessment of mineralogenic toxicity of
aquatic pollutants.
4.000 EUR, Acção Bilateral FCT-CSIC - Efeitos da criopreservação na esqueletogénese e nos
padrões de expressão de genes marcadores ósseos.
-
MechanoCell-ALW-GO-MG/10-07/2009 - Bone Cell Mechanics under Altered Gravity. User
Support Programme Space Research, Netherlands Organization for Scientific Research.
3653-10735/2009 - Identification and characterization of differentially expressed microRNAs in
osteoporotic and healthy postmenopausal women. Norwegian Regional Health Authorities.
198
Fish Nutrition
Fish Nutrition
Aires Manuel Pereira Oliva Teles
Funding in 2009:
-
-
National funding
4.573,78 EUR, FCT POCI/MAR/60895/2004, Effects of complex mixtures of antagonistic
disrupting chemicals in two fish species with different life histories. PI: M Santos (Environmental
Toxicology Lab.). Participating Institutions: CIIMAR; participants of the Laboratory of Nutrition:
AP Carvalho.
Agência de Inovação (Adi), OPTISOLE - Investigação e desenvolvimento na produção de juvenis
de linguado: incremento da resistência a patologias e do crescimento. PI: A Afonso (CIIMAR).
Participating Institutions: CIIMAR; FCUP; ICBAS; Participants of the Laboratory of Nutrition: A
Oliva Teles, H Peres, AP Carvalho, I Guerreiro.
1. Objectives
The fish nutrition group directs its research to the study of basic and applied aspects of the
nutrition and feeding of larvae and juveniles of fish species well established in marine
aquaculture (European sea bass, gilthead sea bream, turbot), candidate species for aquaculture
(Diplodus sp., Senegalese sole), and ornamental species (zebrafish, angelfish, kribensis,
jewelfish).
199
Main research activities are directed towards the study of:
-
Protein and amino acid requirements and metabolic utilization;
-
Utilization of conventional energy sources (lipids and carbohydrates);
-
Phosphorus requirement and bioavailability;
-
Potential use of feedstuffs alternative to fish meal;
-
Nutritional strategies for improvement of fish health and welfare;
-
Development of microparticulate diets for fish larvae and weaning strategies.
These studies aim contributing for a deep insight on the nutritional requirements and metabolic
utilization of nutrients by fish, therefore providing basic information regarding the physiological
aspects of comparative animal nutrition and contributing for a scientific based improvement of
fish diets. From an applied perspective, studies aim maximization of fish growth and feed
utilization while reducing the environmental impact due to feeding. This way it is expected to
contribute for the sustainable development of aquaculture.
During this year studies advanced concerning a deeper understanding of protein nutrition of
Diplodus species, by evaluating protein requirement for growth and maintenance of twobanded sea bream juveniles (Publication nr 5) and the optimum dietary amino acid profile for
gilthead sea bream juveniles (Publication nr 6).
A comparative evaluation of the nutrient digestibility profile of animal by-products in European
sea bass, gilthead sea bream and turbot was also performed aiming the evaluation of the
potential of alternative protein sources to fish meal (Publication nr 1).
Regarding utilization of carbohydrates our efforts prosecuted on studying the use of
carbohydrates of different complexity by gilthead sea bream (Publication nr 12) and the role of
insulin and IGF-I on the response to a glucose load or on the regulation of glucose metabolism in
European sea bass juveniles (2 papers submitted). A review on nutritional regulation of hepatic
glucose metabolism in fish was also published (Publication nr 3).
Aiming the optimization of feed management in aquaculture a study was performed regarding
the effect of feed restriction on performance of turbot in a commercial fish farm (Publication nr
9).
Concerning health effects on fish embryonic development, the toxic effects of cyanobacteria on
early developmental stages of carp (Publications nr 4, 10, 11), on compensatory growth of
zebrafish larvae (Publication nr 2) and of other toxic agents on embryonic development of
zebrafish (Publication nr 8) were also analyzed.
200
8d. Productivity
1. Davies, SJ, Gouveia, A, Laporte, J, Woodgate, SL, Nate, S. 2009. Nutrient digestibility profile of
premium (category III grade) animal protein by-products for temperate marine fish species
(European sea bass, gilthead sea bream and turbot). Aquaculture Research 40(15): 1759-1769.
IF=1.099.
2. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Oudra, B, Del Campo, FF, Vasconcelos, V.
aeruginosa natural bloom extract containing microcystins. International Journal of Molecular
Sciences 10:133-146. IF=1.387.
3. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2009. Nutritional regulation of hepatic glucose
metabolism in fish. Fish Physiology and Biochemistry 35:519-539. IF=1.232.
Ecotoxicology and Environmental Safety 72:473-478. IF=2.133.
5. Ozório, ROA, Valente, LMP, Correia, S, Pousão-Ferreira, P, Damasceno-Oliveira, A, Escórcio, C,
seabream (Diplodus vulgaris) juveniles. Aquaculture Nutrition 15: 85-93. IF=1.482.
6. Peres, H, Oliva-Teles, A. 2009. The optimum dietary essential amino acid profile for gilthead
seabream (Sparus aurata) juveniles. Aquaculture, 296:81-86. IF=1.925.
75, 661-666. IF=3.253.
Guedes-Dias, P, Fontaínhas-Fernandes, A, Parra, SS, Carvalho, AP, Castro, LFC, Santos, MM.
2009. Disruption of zebrafish (Danio rerio) embryonic development after full life-cycle parental
exposure to low levels of ethinylestradiol. Aquatic Toxicology 95:330-338. IF=3.124.
In press
9. Blanquet, I, Oliva-Teles, A. (in press). The effect of feed restriction on performance of turbot
(Scophthalmus maximus) juveniles under commercial rearing conditions. Aquaculture Research.
IF=1.099.
10. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, FF, Oudra, B, Vasconcelos, V. (in
press). Effect of different microcystin profiles on toxin bioaccumulation in common carp
(Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety, Doi:
10.1016/j.ecoenv.2009.12.015. IF=2.133.
201
11. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, FF, Vasconcelos, V, Oudra, B. (in
press). Effects of the microcystin profile of a cyanobacterial bloom on growth and toxin
accumulation in common carp (Cyprinus carpio) larvae. Journal of Fish Biology. IF=1.226.
12. Enes, P, Peres, H, Couto, A, Oliva-Teles, A. (in press). Growth performance and metabolic
utilization of diets including starch, dextrin, maltose or glucose as carbohydrate source by
gilthead sea bream (Sparus aurata) juveniles. Fish Physiology and Biochemistry, Doi:
10.1007/s10695-009-9366-y. IF=1.232.
proceedings)
proceedings)
PhD THESES
Laporte, J. 2009. Nutritional evaluation of animal by-products for the partial replacement of fish
meal in diets for gilthead sea bream Sparus aurata. PhD thesis, School of Biological Sciences,
Faculty of Sciences, University of Plymouth, UK. Supervisor: S Davies (University of Plymouth),
Co-supervisor: A Gouveia.
Research cooperation is well established with:
-
Prof. SJ Davies, Faculty of Sciences, Plymouth University
-
Dr. SJ Kaushik, INRA, St. Pée sur Nivelle, France
-
Dr. S. Panserat, INRA, St. Pée sur Nivelle, France
202
-
Prof. AE Morales, Faculty of Sciences, Granada University
-
Prof. Vera Cruz Rubio, University of Murcia
-
Prof. J Gutierrez, University of Barcelona
-
Prof. A Krogdahl, Norwegian Science and Veterinary Science, Aquaculture Protein Center
Fish Nutrition participated in the meetings promoted by the North Regional Coordination and
8e. Future research
1. Objectives
Research within the next few years will continue to fall across the main research areas that were
defined in our group as research priorities.
Research focused in commercial aquaculture species such as sea bass and sea bream juveniles
will be focused on:
-
Deeper analysis on amino acid requirements and amino acids utilization;
-
Further understanding of hormonal regulation of carbohydrate metabolism;
-
Evaluation of the antioxidative stress due to feedstuffs and additives and its effect on
the innate defense mechanisms;
-
Evaluation of feedstuffs utilization and the effect of anti-nutrients on gut morphology,
physiology and health;
-
Use of probiotics and prebiotics and their effect on gut microbiota and the immune
system;
-
Further understanding of phosphorus utilization and uptake regulation.
In new species for aquaculture aim to study:
-
The effect of temperature on growth and nutrient utilization of Senegalese sole;
-
The utilization of carbohydrate rich plant feedstuffs in omnivorous species such as
Diplodus sargus and carnivorous species such as Senegalese sole;
203
-
Establish the macronutrient requirements and utilization of new species for
aquaculture, such as Diplodus cervinus, Diplodus puntazzo and Solea senegalensis;
-
Utilization of alternative protein sources to fish meal.
Such studies will be carried out using conventional approaches already in use in our lab but also
molecular approaches, namely nutrigenomics and proteomics that may give a new insight in the
elucidation of such aspects. Aspects related to nutritional impact on fish health will also be
prosecuted.
All this implies a multidisciplinary approach, therefore a close collaboration with colleagues both
from CIIMAR and external laboratories (national and international) will be necessary.

Current funding
Agência de Inovação (Adi), OPTISOLE - Investigação e desenvolvimento na produção de juvenis
de linguado: incremento da resistência a patologias e do crescimento. PI: A Afonso (CIIMAR).
Participating Institutions: CIIMAR; FCUP; ICBAS; participants of the Laboratory of Nutrition: A
Oliva Teles, H Peres, AP Carvalho.
204
Fisheries Biology and Hydroecology Research Group (FBHRG)
Fisheries Biology and Hydroecology Research Group (FBHRG)
José Pedro de Andrade e Silva Andrade
3. Location of group (Host institution)
Seahorse research during 2009 was entirely supported by the FCT plurianual funding allocated
to JP Andrade and J Palma - 3202.52 EUR.
During 2009, cuttlefish related research was funded as follows:
-
5.735 EUR, ASSEMBLE - EC/FP7/227799, 4 year Joint Research 1 Task within the project,
the project started on March 2009.
-
1601,26 EUR FCT Pluriannual funding.
Total funds attained 7336.26 EUR, from which approximately 80% corresponded to EU Funding
Schemes Research Projects and the remaining to FCT pluriannual funding.
During this year, we also received approval for funding (189.702 EUR) of a National FCT project,
SEPIAMETA (PTDC/MAR/102348/2008), which will start on January 2010.
1. Objectives
Regarding seahorse culture, the 2009 objectives were as follows:
-
To determine and evaluate the use of enriched shrimp as a diet for long snout seahorse
(Hippocampus guttulatus);
-
To determine the effect of broodstock nutrition on the long snout seahorse
(Hippocampus guttulatus) reproduction and brood quality;
205
-
Evaluate the apparent digestibility coefficients of various feed ingredients for adult;
-
To optimize Artemia enrichment and feeding protocol when rearing juvenile long snout
seahorse, Hippocampus guttulatus;
-
To define the courtship and the breeding behaviour of the long snout seahorse
(Hippocampus guttulatus).
Regarding cuttlefish culture, the 2009 objectives were as follows:
-
To determine the suitability of an early weaning in the existent cuttlefish culture
protocol, using frozen grass shrimp, and its effects on growth and mortality during the
hatchling stage and the transition to the juvenile stage;
-
To determine the possibility of increasing cuttlefish fecundity and fertility under
captivity through the use of increased bottom areas.
Seahorse broodstock management:
-
That is possible to combine the use of a natural diet (shrimp) filled with an artificial diet
as it is a practical co-feeding of two diets in just one item, and can be regarded as a step
ahead in seahorse nutrition.
-
Seahorses fed with a combination of a natural and artificial diet, generated better
broods and more fitted descendents, fact that is extremely relevant within the seahorse
nutrition and production.
-
It was possible to thoroughly define both the courtship and breeding behaviour of long
snout seahorse, Hippocampus guttulatus. These findings are extremely valuable as they
can be used a toll both for conservation and for broodstock management in a
commercial perspective.
Seahorse Juvenile Stage:
-
Our studies contributed to determine the adequate Artemia enrichment and to
establish a successful feeding plan when rearing juvenile long snout seahorse,
Hippocampus guttulatus from hatch. The obtained results are the first record of
successful breeding of H. guttulatus in captivity and are a promising starting point to
improve the rearing techniques to successfully breed this candidate species to
aquaculture.
Cuttlefish Hatchling Stage:
-
206
Cuttlefish can be weaned on frozen grass shrimp from the first day after hatching,
despite earlier contrary reports;
-
This facts is considered particularly relevant because it results from prior zoo-technology
optimization of culture protocol at this life stage regarding tank color and light
intensities; and because it points to a possible acceptance of a well designed prepared
diet at this stage.
Cuttlefish Adult Stage:
-
Although still under analysis, results indicate that an increase of the available bottom
area will promote an increase in fecundity and fertility. Despite the preliminary nature
of this finding, the possibilities of increasing offspring production to values similar to
those commonly found in nature are now high.
8d. Productivity
The FBHRG has published a total of 5 ISI-listed peer-reviewed articles, from which 80% resulted
from international collaborations. The mean Impact Factor (based on ISI JCR 2008) for those 5
publications was 1.767. From the total published articles, one is within the top 10 and another in
the top 20 threshold value for the “Fisheries” subject category (ISI JCR 2008), one in the top 30
threshold value for the “Food Science and Technology” and another in the top 50 threshold
value for the “Biotechnology and Applied Microbiology” of ISI JCR 2008. During this year, the
FBHRG has published 1.25 publications (ISI) per FBHGR member with a PhD degree.
1. Esteves, E, Pina, T, Andrade, J.P. 2009. Diel and seasonal changes in nutritional condition of
the anadromous Twaite shad Alosa fallax fallax (Lacepede, 1803) larvae. Ecology of Freshwater
Fish 18: 132-144. IF=1.512.
2. Palma, J, Bureau, DP, Correia, M, Andrade, JP. 2009. Effect of temperature, density and early
weaning in survival and growth of Palaemonetes varians larvae. Aquaculture Research 40(13):
1468-1473. IF=1.099.
3. Sykes, AV, Oliveira, AR, Domingues, PM, Cardoso, CM, Andrade, JP, Nunes, ML. 2009.
Assessment of European cuttlefish (Sepia officinalis, L.) nutritional value and freshness under ice
storage using a developed Quality Index Method (QIM) and biochemical methods. LWT - Food
Science and Technology 42: 424-432. IF=2.114.
4. Sykes, AV, Almansa, E, Lorenzo, A, Andrade, JP. 2009. Lipid characterization of both wild and
cultured eggs of cuttlefish (Sepia officinalis) throughout the embryonic development.
In press
5. Hormiga, JA, Almansa, E, Sykes, AV, Torres, N. (in press). Model based optimization of feeding
regimens in aquaculture: Application to the improvement of Octopus vulgaris paralarvae
viability in captivity. Journal of Biotechnology. Doi: 10.1016/j.jbiotec.2009.12.008. IF=2.881.
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proceedings)
proceedings
MASTER THESES
Gomes, MS. 2009. Determination of optimal larval rearing condition for broad noosed pipefish
(Syngnathus typhle, Linnaeus, 1758) (Pisces: Syngnathidae). Master thesis, University of Algarve.
Co-supervisor: JP Andrade, J Palma.
In the last 3 years, the FBHRG established contacts to bring together research groups interested
in seahorse research. These include other non-European and European laboratories, namely the
University of Guelph (Department of Animal and Poultry Science), Canada, which granted a
fructiferous cooperation regarding some seahorse nutrition issues, the Toronto Zoo, Canada,
with an exchange of husbandry protocols, the Zoological Society of London and the Oceanário
de Lisboa under the same scope and finally the Project Seahorse as a research partner. A
memorandum of understanding establishing the terms of reference for future joint seahorse
research has been signed between the FBHRG and the Project Seahorse.
This group also established research contacts with other European and non-European
laboratories in order to prepare a proposal to establish the European Network for Cephalopod
Aquaculture Development (ENCAD). Nationally, we have been cooperating with the IPIMAR.
Internationally, we have been collaborating actively with the IFAPA – Agua del Pino Research
Centre (Huelva, Spain) as well with the Laboratory of Animal Physiology from the University of
La Laguna (Tenerife, Spain), exchanging both students and researchers that result in the
collaborative publications that are listed above. Nonetheless, we have also established research
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contacts with other European and International laboratories in Spain, France, UK, Germany,
Italy, Croatia, Mexico, Chile, etc. These International contacts are being made for the last 2 years
as part of an FP7 theme for Project proposal that was submitted in 2009. We also applied for
European network funding twice this year, through COST. Unfortunately, all international
proposals were not successful.
8e. Future research
1. Objectives
The current and future objectives regarding the husbandry and nutrition of the long snout
seahorse, Hippocampus guttulatus for the next 3 year period, include:
-
To determine the influence of pre-inductive photoperiod variations on long snout
seahorse (H. guttulatus) broodstock response to an inductive photothermal program;
-
To determine the effects of photoperiod and light intensity on growth, survival and
activity of juvenile long snout seahorse (H. guttulatus);
-
To determine the foraging, growth and survival of juvenile long snout seahorse (H.
guttulatus) reared in different prey concentrations;
-
To determine the impact of dietary supplementation with astaxanthin on egg and larval
quality in long snout seahorse (H. guttulatus);
-
To study the ontogenetic development of the digestive tract in reared long snout
seahorse (H. guttulatus) larvae;
-
To determine the digestibility and protein utilization by juvenile long snout seahorse (H.
guttulatus);
-
To determine the digestibility and lipid utilization by juvenile long snout seahorse (H.
guttulatus);
-
To determine the effect of lysine supplementation in the amino acid metabolism of
juvenile long snout seahorse (H. guttulatus) fed Artemia;
-
To determine the dietary lysine requirement of juvenile long snout seahorse (H.
guttulatus).
Future seahorse research will also include the study of social and sexual interactions of
Hippocampus guttulatus and H. hippocampus from the Ria Formosa, combining in-situ and
ex-situ experiments using microsatellites. Topics of research will include:
209
-
Analysis of paternity on wild populations of both seahorse species and an analysis of
paternity of wild animals kept in captivity under controlled conditions;
-
The effects of manipulating the operational sex ratio and the influence of female size on
male mating decisions.
Finally, seahorse research will also include the study of the ecology of H. guttulatus and H.
hippocampus in the Ria Formosa, with a special emphasis on the causes for the decline of
population numbers registered during the last 4-6 years. Miguel Correia (MSc) will address these
topics during his PhD project: Long-snouted seahorse Hippocampus guttulatus (Pisces:
Syngnathidae) restocking management in the South coast of Portugal (ref
SFRH/BD/41020/2007).
With respect to cephalopod and cuttlefish (S. officinalis) culture, current and future objectives
will be focused on the zoo-technology, nutrition and genetics (with a special emphasis on the
analysis of inbreeding in consecutive cuttlefish generations produced in captivity). The
objectives for a 3-year period are as follows:
-
To determine the nutritional and energy content of grass shrimp;
-
To identify cuttlefish enzyme activity during digestion during every life stage;
-
Based on the previous, to establish possible metabolic pathways;
-
To develop a first successful artificial diet for the species;
-
To understand the influence of several factors on the reproduction of cuttlefish in
captivity, to establish a new breeder protocol for the species based on a
multidisciplinary approach of the currently identified variables;
-
To determine the feasibility of cuttlefish industrial production.
To achieve these objectives we will continue to expand the number of national and international
researchers associated to these tasks. We will also expand collaboration within CCMAR, and at
national and international level.
This group also plans to extend its research theme to the development of aquaculture
technology to other cephalopod species, acting as consultant for worldwide laboratories.
The FBHRG is also aiming at establishing a practical, optimized procedure for fertilization and
incubation of eggs and hatching, rearing and marking of twaite shad Alosa fallax fallax, larvae
that is suitable for future up-scaling from laboratorial settings ,to an aquaculture production
level capable of sustaining an eventual stock-enhancement plan, namely the optimization of
stripping of adult spawners, the identification of adequate (live-)food for larvae, the
examination of life-history traits (nutritional condition, growth rate and mortality rate) and the
optimization of marking method for re-capture purposes. In this context, Eduardo Esteves
submitted (as the PI) to FCT (MCTES), on February 2009, the proposal “Culture of twaite shad
Alosa fallax fallax (Lacepede, 1803) larvae for stock enhancement: study and optimization of
rearing conditions”: requested funding: 94.293 EUR. Despite the “not recommended for
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funding” decision, the FCT (MCTES) board of reviewers concluded that “This project (…) is
important for ecological and conservation goals. (…) there is still a challenge in determining the
optimum conditions for a new species and these investigations could be productive in terms of
experimental results. (…) There is an interesting interaction between field and lab tasks.
However, (…) better and probably more feasible focus would have been to characterize
differences in spawning characteristics of the river populations, and early life history traits of
significance to "optimizing" incubation and feeding protocols.” The PI is in the process of
reformulating and preparing the proposal for the next call.
Contributing to the study of aspects related to quality and safety of seafood (e.g. cephalopods,
bivalves, shrimp), namely the examination of consumption statistics, the study of spoilage
dynamics of various physico-chemical and microbiological characteristics, the application of
sensory analysis and assessment of freshness, products’ authenticity, public health issues and
environmental impact are topics for the future research of the FBHRG.

Current funding
Cephalopod related research will be funded in the next years by the following projects:
-
5.735 EUR, EC/FP7/227799, ASSEMBLE, 3/2009-2/2013.
-
189.702 EUR, FCT PTDC/MAR/102348/2008, SEPIAMETA, 1/2010-12/2012. PI: A Sykes.
The FBHRG is also involved in the project EEMA - Avaliação do Estado Ecológico das Massas de
Água Costeiras e de Transição e do Potencial Ecológico das Massas de Água Fortemente
Modificadas, with a funding allocated to this research group of 18.322 EUR. 09/2009-08/2011.
PI: J Andrade

Pending funding
Within the seahorse line of research, funding for the following three proposals is still pending
(FCT call 2009):
-
123.595 EUR, From abundance to disappearance. Identifying causes for Hippocampus
guttulatus population decrease in the Ria Formosa lagoon. PI: J Palma.
-
184.843 EUR, Breeding long snout seahorse (Hippocampus guttulatus): a tool for
conservation and knowledge. PI: J Palma.
-
175.697 EUR, Sexual selection in Hippocampus guttulatus and H. hippocampus: insights
from in situ and ex situ studies using microsatellite-based parentage analysis. PI: JP
Andrade
This line of research has pending funding regarding the following two proposals:
211
-
292.219 EUR, PROMAR: 31.03.05.FEP.002, SEPIATECH, 4 year national DGPA project.
The project is on the final stages of approval and is expected to start in June-July 2010.
PI: JP Andrade.
-
199.947 EUR, PTDC/MAR/110533/2009, SEPIABREED. The project is currently under
evaluation and is expected to start onSeptember 2010. PI: A Sykes.
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Karim Erzini
CCMAR - centro de Ciências do Mar, Faro
-
25.000 EUR FCT pluriannual funding.
-
National funding
124.916 EUR, RENSUB IV, ARH Algarve. Cartography and characterization of the marine
communities of the National Underwater Ecological Reserve between the Arade River and Ponta
da Piedade. 2009-2011.
71.710 EUR, INAG, EMMA - Avaliação do Estado Ecológico das Massas de Água Costeiras e de
Transição e do Potencial Ecológico das Massas de Água Fortemente Modificadas. 2009-2010.
9.300 EUR, FCT POCI/BIA-BDE/59426/2004, LobAssess, 2005-2009.
60.720 EUR, FCT PDCT/MAR/59366/2004, SURVIVAL, 2007-2009.
-
254.388 EUR, LIFE Project - PTCON0010, EU /Secil, Restoration and Management of Biodiversity
in the Marine Park Site Arrábida-Espiche, 2007-2010.
Ocean Tracking Network - OTN. Canada Foundation for Innovation $ 35 million. Project will
provide telemetry equipment (unspecified amount - still being negotiated), 2007-2011.
213
1. Objectives
Fishing and fishing related activities are of considerable importance in the Algarve (southern
Portugal), which is the Portuguese region with the highest percentage of the working population
directly or indirectly involved in fisheries. The main objectives of the group are to contribute to
the improved management and sustainable exploitation of the multi-species resources of the
Algarve coastal waters. In recent years, the fisheries sector has been under considerable
pressure, with a decline in landings of many of the most important species. Within this context,
the main objectives of the group are to contribute to the improved management and
sustainable exploitation of the multi-species resources of the Algarve coastal waters. Specific
objectives include:
-
Studying the impact of commercial fishing activity and the implications for biodiversity:
species and size selectivity of different gears, quantification of the by-catch and discards
of different gears, and evaluation of ghost fishing;
-
Studying the impact of recreational fishing activity;
-
Finding ways to mitigate by-catch, discarding and to propose management measures
and changes in legislation based on the above mentioned studies;
-
Biology, population dynamics, and ecology of fish and invertebrates: growth and
mortality parameters, maturity and reproduction, feeding ecology;
-
Identification of essential fish habitat, by classification of the bottom, mapping of the
biodiversity and distribution and abundance of key species;
-
Long-term monitoring of key habitats and recruitment of fish and invertebrates;
-
Evaluation of the effects of marine protected areas;
-
Understanding the spatio-temporal dynamics of key commercial species by means of
tagging and telemetry studies;
-
Modeling and simulation studies for spatio-temporal dynamics, eco-trophic modeling,
evaluating the effects of different management measures, and understanding the
influence of environmental and fisheries factors.
In 2009, work continued on the mapping of the Algarve coast to a depth of 30m, with
classification of the bottom and identification of fish, invertebrates and algae, including the
description of new species (e.g. Fusinus albacarinoides) and new records for Portugal and
Europe.
214
A PhD on underwater ecotourism completed the analysis on the scuba diving socio-economic
profile in the Algarve and formulated several underwater routes based on biodiversity
conservation criteria which were in place and evaluated.
The group has now completed a series of projects spanning a period of more than 10 years on
the impacts in terms of by-catches and discarding, species and size selectivity of all the main
gears used in the Algarve. For some gears (deepwater hake longline, demersal purse seine and
bottom trawling), gear modifications for the reduction by-catch have been successfully tested,
with the collaboration of commercial fishermen and other research institutions such as the
IPIMAR.
The biology, population dynamics and ecology of a number of species of fish, cephalopods and
crustaceans have been studied, namely commercially important sea breams (Sparidae),
deepwater sharks, octopus and Norway lobster. Estimated parameters have been used in stock
assessments for some species and for simulation based studies to evaluate different
management measures.
A PhD project on recreational / sport fishing was continued in 2009, with the development of
new experimental fishing trials within a large earth tank which allowed some direct estimates of
hook selectivity.
Another PhD project is using acoustic telemetry to study spatio-temporal dynamics and habitat
use of key species in the Arrábida marine park, namely an herbivore fish species, Sarpa salpa.
Annual monitoring of key nursery habitats: Ria Formosa lagoon (since 2001) and Arade and
Guadiana river estuaries (since 2003 and 2009, respectively) has been carried out. These time
series will provide important information on recruitment variability and will be useful for
evaluating climate change effects.
Monitoring of fish and invertebrates in different protection areas of the Arrábida Marine
Protected Area continued in 2009, along with onboard monitoring of commercial fishing activity.
Alternatives to traditional management based on biological indicators, and management
actively involving fisherman are being tested through collaborations with other research groups,
and are part of a series of Master and PhD thesis.
A number of projects were submitted and new projects were initiated.
8d. Productivity
14 publications in 2009:
1. Assis, J, Tavares, D, Tavares, JT, Cunha, AH, Serrão, E. 2009. Findkelp, a GIS-based community
participation project to assess Portuguese kelp conservation status. Journal of Coastal Research
56:1469-1473. IF=1.366.
215
2. Abecasis, D, Bentes, L, Erzini, K. 2009. Home range, residency and movements of Diplodus
sargus and Diplodus vulgaris in a coastal lagoon: connectivity between nursery and adult
habitats. Estuarine, Coastal and Shelf Science 85: 525-529. IF=1.970.
3. Abecasis, D, Cardigos, F, Almada, F, Gonçalves, JMS. 2009. New records on the ichthyofauna
of the Gorringe seamount (Northeastern Atlantic). Marine Biology Research 5: 605-611.
IF=1.000.
4. Cunha, AH, Araújo, A. 2009. New distribution limits of seagrass beds in West Africa. Journal of
Biogeography 36:1621-1622. IF=4.087.
5. Cunha, AH, Assis, J, Serrão, E. 2009. Estimation of available seagrass meadow area in Portugal
for transplanting purposes. Journal of Coastal Research 56: 1100-1104. IF=1.366.
6. Czerwinski, IA, Erzini, K, Gutiérrez-Estrada, JC, Hernando, JA. 2009. Deep water longline
selectivity for black spot seabream (Pagellus bogaraveo) in the Strait of Gibraltar. Fisheries
Science 75: 285-294. IF=0.684.
7. Hadorn, R, Afonso, CML, Rolán, E. 2009. A new Fusinus (Gastropoda: fasciolariidae) from the
Algarve, south coast of Portugal. Iberus 27(1): 119-129.
8. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2009. Diplodus spp. assemblages on artificial
reefs: importance for near shore fisheries. Fisheries Management and Ecology 16: 88-99.
IF=1.264.
9. Oliveira, F, Almeida, C, Gonçalves, JMS. 2009. Ascidonia flavomaculata (Heller, 1864), a new
record for the Portuguese marine invertebrate fauna (Decapoda, Pontoniidae). Crustaceana
82(1): 63-67. IF=0.507.
10. Vasconcelos, P, Gaspar, MB, Castro, M, Nunes, ML. 2009. Influence of growth and
reproductive cycle on the meat yield and proximate composition of Hexaplex trunculus
(Gastropoda: Muricidae). Journal of the Marine Biological Association of the United Kingdom 89:
1223-1231. IF=0.995.
11. Veiga, P, Machado, D, Almeida, C, Bentes, L, Monteiro, P, Oliveira, F, Ruano, M, Erzini, K,
Gonçalves, JMS. 2009. Weight-length relationships for 54 species of the Arade estuary, south
Portugal. Journal of Applied Ichthyology 25: 493-496. IF=1.121.
In press
12. Cunha, AH, Santos, R. (in press). The use of fractals to assess seagrass landscape stability: a
case study from the barrier island system of Ria Formosa (South of Portugal). Estuarine, Coastal
and Shelf Science. Published online, 4 August 2009, Doi:10.1016/j.ecss.2009.07.030. IF=1.970.
13. Marçalo, A, Marques, TA, Araújo, J, Pousão-Ferreira, P, Erzini, K, Stratoudakis, Y. (in press).
Fishing simulation experiments for predicting the effects of purse-seine capture on sardine
(Sardina pilchardus). ICES Journal of Marine Science. Published on-line, 4 November 2009, Doi:
10.1093/icesjms/fsp244. IF=1.920.
216
14. Monteiro, P, Bentes, L, Coelho, R, Correia, C, Erzini, K, Lino, P, Ribeiro, J, Gonçalves, JMS. (in
press). Age and growth, mortality and reproduction of the striped sea bream, Lithognathus
mormyrus Linnaeus 1758, from the south coast of Portugal (Algarve). Marine Biology Research.
Published on-line, 19 November 2009, Doi: 10.1080/17451000903039731. IF=1.000.
proceedings)
Book (Chapters)
1. Lino, PG, Bentes, L, Abecasis, D, Santos, MN, Erzini, K. 2009. Comparative behavior of wild and
hatchery reared white seabream (Diplodus sargus) released on artificial reefs off the Algarve
(southern Portugal). In: Nielsen, JL, Arrizabalaga, H, Fragoso, N, Hobday, A, Lutcavage, M, Sibert,
J (Eds). Tagging and Tracking of Marine Animals with Electronic Devices - Reviews: Methods and
Technologies in Fish Biology and Fisheries, vol. 9, pp. 23-34. ISSN: 1571-3075.
proceedings)
1. Cunha, AH, Paulo, D, Boavida, J, Rodrigues, S, Pires, F, Sayanda, D, Fonseca, M. 2009. Action
C.1 - Collection, preparation and planting of seagrass transplantation unit. Progress report for
Action C.1, for the period from January to December, 2009. Biomares LIFE06 NAT/P/000192.
Centro de Ciências do Mar, 14 pp.
2. Cunha, AH, Paulo, D, Boavida, J, Rodrigues, S, Pires, F, Sayanda, D, Fonseca, M. 2009. Action
D.2 - Conduct monitoring of seagrass populations in the restored and donor areas pre and post
habitat restoration to track recovery. Progress report for Action D.2, for the period from January
to December 2009. Biomares LIFE06 NAT/P/000192. Centro de Ciências do Mar, 58 pp.
3. Cunha, AH, Erzini, K, Serrão, E, Gonçalves, E, Henriques, M, Henriques, V, Guerra, M, Duarte,
C, Marb, N, Fonseca, M. 2009. Biomares a LIFE project to restore and manage the biodiversity of
Luiz Saldanha Marine Park. Progress Report NΊ2 (January-December 2008). Biomares LIFE06
NAT/P/000192. Centro de Ciências do Mar, 44 pp.
4. Cunha, AH, Erzini, K, Serrao, E, Gonçalves, E, Henriques, M, Henriques, V, Guerra, M, Duarte,
C, Marb, N, Fonseca, M. 2008. Biomares a LIFE project to restore and manage the biodiversity of
Luiz Saldanha Marine Park. Interin Report NΊ2 (January-December 2007 and 2008). Biomares
LIFE06 NAT/P/000192. Centro de Ciências do Mar, 44 pp.
5. Cunha, AH, Erzini, K, Serrao, E, Gonηalves, E, Henriques, M, Henriques, V, Guerra, M, Duarte,
C, Marb, N, Fonseca, M. 2008. Biomares um projecto LIFE para a recuperação e gestão da
biodiversidade do Parque Marinho Luiz Saldanha. Relatório Não-Técnico NΊ2 (Janeiro a
Dezembro de 2008). Biomares LIFE06 NAT/P/000192. Centro de Ciências do Mar, 29 pp.
217
MASTER THESES
Claro, B. 2009. Caracterização sócio-económica do turista de observação de cetáceos do Algarve
e determinação do seu grau de satisfação e perfil ecológico. Master thesis in Marine Biology,
University of Algarve. Supervisors: M Castro, Sara Magalhães (Mar Ilimitado, Sagres).
University of the Algarve. Supervisor: Alberto Correia (Ecophysiology Group, CIIMAR), Cosupervisor: K Erzini.
Inácio, MIM. 2009. Transporte de crustáceos vivos em ambiente refrigerado. Master thesis in
Marine Biology, University of Algarve. Supervisors: M Castro, Aida Campos (IPIMAR).
Leocádio, AM. 2009. Economic and biologic evaluation of two métiers, creels and bottom trawl,
for catching Norway lobster off the Portuguese coast. International Master thesis in Aquaculture
and Fisheries, University of Algarve. Supervisors: M Castro, David Withmarsh (University of
Portsmouth, UK).
Mateus, M. 2009. Characterization of the epibenthic macro-invertebrate community of the
Arade estuary. Master thesis, Faculty of Marine Sciences and Environment, University of
Algarve. Supervisors: JMS Gonçalves and K Erzini.
Miodonski, J. 2009. Caracterização da Ictiofauna de intertidal rochoso na costa sul de Portugal.
Master thesis, Faculty of Marine Sciences and Environment, University of Algarve. Supervisors:
JMS Gonçalves and K Erzini.
Supervisor: Alberto Correia (Ecophysiology Group, CIIMAR), Co-supervisor: K Erzini.
Santinho, CIA. 2009. Padrões de distribuição da abundância larvar de crustáceos decápodes na
baía de Cascais. Master thesis in Marine Biology, University of Algarve. Supervisors: M Castro,
Antonina dos Santos (IPIMAR).
Vicente, AVH. 2009. Manutenção de lagostins (Nephrops norvegicus) vivos, a bordo de arrastões
comerciais. Master thesis in Marine Biology, University of Algarve. Supervisors: M Castro, Aida
Campos (IPIMAR).
Vieira, P. 2009. Caracterização das espécies de Gorgónias (Cnidaria, Gorgonacea) da costa
Algarvia. Master thesis, Faculty of Marine Sciences and Environment, University of Algarve.
Supervisors: JMS Gonçalves and L Fonseca.
218
PhD THESES
Leitão, FMS. 2009. Algarve Artificial reefs colonization processes and fish trophic ecology:
implications for the local near shore fisheries. PhD thesis, University of Algarve. Supervisor: K
Erzini, Co-supervisor: MN dos Santos (IPIMAR).
Marçalo, ALB. 2009. Sardine (Sardina pilchardus) delayed mortality associated with purse seine
slipping: contributing stressors and responses. PhD thesis, University of Algarve. Supervisor: K
Erzini, Co-supervisor: Y Stratoudakis (IPIMAR).
-
Organization and hosting of the Faro Ocean Tracking Network (OTN), 3-5 December,
Faro, Portugal. Workshop leader: Dr. Ron O’Dor. Number of participants: 65.
-
Jornadas de Classificação e Análise de dados JOCLAD 2009, Faro, Portugal. Member of
the Organizing Committee.
2007-2010. The BIOMARES LIFE project is partly funded (50%) by SECIL, the cement
manufacturer.
International research collaboration
-
Ocean Tracking Network (http://oceantrackingnetwork.org/), global partnership.
-
BIOMARES LIFE proj: USA (NOAA), Spain (CSIC).
-
Working Group on Habitat Mapping of ICES.
-
EUNIS habitat classification system: Ifremer (France), Marine Inst (Ireland), JNCC (UK),
AZTI and IEO (Spain).
-
Human Dimensions Research Lab, Texas A&M Univ (recreational fishing research).
-
Florida Program for Shark Research, Univ Florida, USA and the Federal Rural Univ
Pernambuco, Brazil.
International meeting / symposia
-
2nd Australian Acoustic Tracking and Monitoring System workshop, 2-4 Nov, Sydney,
Australia.
219
-
8th Indo Pacific Fish Conference and 2009 ASFB Workshop & Conference, 31-May-05
June, Australia.
-
8th Conference on Fish Telemetry, 14-18 Sept, Sweden.
-
3rd Estuarine & Coastal Sciences Association International Symposium, 7-9 Feb, Lisbon,
Portugal.
-
ICES Working Group on Quantifying all Fishing Mortality, 24-26 Nov, Copenhagen,
Denmark.
-
25th American Elasmobranch Association Meeting, 22-27 July, Portland, USA.
-
Mediterranean Seagrass Biology Workshop, Croatia, 1-5 Sept 2009.
-
International Meeting on Marine Resources, 16-18 Nov, Peniche, Portugal.
-
Coastal and Estuaries Research Federation Annual Meeting, 1-5 Nov, Portland, USA.
-
29th Annual Meeting of the Florida Chapter of the American Fisheries Society, 17-19
Feb, Ocala, Florida, USA.
-
9th International Conference on Artificial Reefs and Related Aquatic Habitats, 8-13 Nov,
Brazil.
-
2nd Marine Biology Congress, 24-28 May, Brazil.
Visiting scientists
-
Ron O'Dor (Dalhousie Univ - Canada, Dir. Census of Marine Life and the Ocean Tracking
Network).
-
Maroune Bdioui and Lotfi Ben Naceur (National Inst of Fisheries, Tunisia).
Graduate training
-
Coordination of Erasmus Mundus MSc in Marine Biodiversity and Conservation,
International MSc in Aquaculture and Fisheries.
Teaching (Univ Algarve):
-
Erasmus Mundus MSc in Marine Biodiversity and Conservation, International MSc in
Aquaculture and Fisheries, MSc in Marine Biology.
-
Statistical analysis, Management and Conservation, Marine Population Dynamics, Stock
Assessment, Fisheries Technology, Modelling in Fisheries, Underwater Methods for
Marine Biodiversity.
220
Other Graduate teaching:
-
MSc in Marine Resources and Coastal Management (Univ Cabo Verde): Conservation
and Management, Statistical Analysis, Fisheries Technology and Modeling in Fisheries.
-
International MSc in Aquaculture and Fisheries (Univ Cádiz): Selectivity of fishing gear.
With the objective of contributing to the management of the underwater (from 0 to 30 m
depth) component of the National Ecological Reserve the subtidal marine communities of the
Algarve are being mapped. In 2009 the area between Portimão and Lagos was initiated
(Cartography and characterization of the marine communities off the National Underwater
Ecological Reserve between the Arade river and Ponta da Piedade (RENSUB IV). This study is
funded by the regional administration (Administração da Região Hidrográfica do Algarve, ARH
Algarve) and is intended to help implement a spatial planning of the marine environment which
could minimize potential conflicts between the variety of activities taking place in this particular
coastal zone.
The BIOMARES LIFE project (2007-2010), is partly funded (50%) by SECIL, the cement
manufacturer.
8e. Future research
1. Objectives
Marine Protected Areas (MPA)
-
Within the context of the BIOMARES project (2007-2010), the group will focus on
evaluating the effects of different levels of protection within the Arrábida marine park
(total, partial and complementary). Regular monitoring using a variety of gears is being
carried out. Indicators will be calculated from these data (e.g. density of key species,
mean sizes). Acoustic telemetry will be used to study spillover or the contribution of the
MPA to the adjacent non-protected areas. Key species to study include several sea
breams, soles, bass and cuttlefish. The role of herbivory within the MPA will also be
studied, focusing on the biology and ecology of the dominant herbivore fish species,
Sarpa salpa.
Spatio-temporal dynamics and habitat use
-
In addition to the telemetry studies in the MPA, the group will continue acoustic
telemetry studies in Algarve coastal waters, with emphasis on artificial reef related work
in collaboration with IPIMAR. Specifically, the goals are to evaluate the use of artificial
reefs (the largest area in European waters) by wild and cultured (stocked) sea breams.
Through the global Ocean Tracking Network project, the group will participate in the
221
deployment of arrays of hydrophones across the Strait of Gibraltar and the continental
shelves of southern Portugal and north-western Morocco. These arrays will eventually
allow the monitoring of movements of highly migratory species. A PhD student, D.
Abecasis, is using acoustic telemetry to study spatio-temporal dynamics and habitat use
of key species in the Arrábida marine park.
Seabed classification, mapping and essential fish habitat
-
In the next few years the group will finish mapping the entire Algarve coastal waters to a
depth of 30m using divers, video camera mounted on a towed sledge and beam trawl.
The data is being implemented in GIS and will be used develop indicators and for
modeling (e.g. GAMs), allowing the identification of biodiversity hotspots and essential
fish habitat. The group is going to participate in a major pan-European proposal focusing
on sea bed mapping and biodiversity: Atlantic Area Transnational Programme 20091/110, MESH ATLANTIC: Mapping Atlantic Area seabed habitats for better marine
management.
Monitoring of key habitats
-
In addition to the monitoring in the Arrábida MPA, the group will continue their longterm annual monitoring programs of the ichthyofauna of the Ria Formosa lagoon (since
2001), the Arade and Guadiana River estuaries (since 2003 and 2009, respectively).
Sport fishing
-
Having completed a study of shore-based rod and line sport fishing, along 250 km of
coastline from Sines to Vila Real de Stº António, the group will now focus on boat-based
sport fishing and underwater spear fishing. Experimental fishing trials within a large
earth tank were carried out and direct estimates of hook selectivity were obtained for
the first time.
Fisheries management, population dynamics and conservation
-
The group will continue work contributing to ensuring the sustainability of Portuguese
fisheries. In this context, ongoing studies are evaluating of alternative, less damaging
fishing gears, in specific fisheries. Simulation and modeling studies will also be used to
evaluate different management scenarios. Ongoing bio-economic studies will also
contribute to providing the basis for improved management and conservation of
fisheries resources. Population dynamics and demographic analysis of top predators,
especially sharks, are also a priority.

Current funding
FCT pluriannual funding.
124.916 EUR, RENSUB IV, ARH Algarve. Total funding, 2009-2011.
222
71.710 EUR, INAG, EMMA, 2009-2010.
254.388 EUR, LIFE Project - PTCON0010, EU /Secil, 2007-2010.
Ocean Tracking Network - OTN. Canada Foundation for Innovation $ 35 million, 2007-2011.

PROMAR Program (DGPA/UE).
199.470 EUR, Promar, eixo 3, DGPA, SPEARFISHING - Avaliação da pesca submarina em Portugal
continental.
118.050 EUR, Promar, eixo 3, DGPA, PESCABORDO - Estudo da pesca recreativa embarcada na
costa sul de Portugal.
-
FCT
198.192 EUR, PTDC/MAR/104113/2008, REEFMAP - Mapping marine priority reef habitats:
biodiversity and fisheries.
120.230 EUR, FISHIMPACT - Trammel net fishery impacts on marine ecosystems.
141.588 EUR, PTDC/MAR/103736/2008, Habitat use and adaptive management of a marine
protected area.
114.939 EUR, PTDC/MAR/103173/2008, Recreational boat fishing: impacts on living resources
and implications for sustainability and management.
173.662 EUR, PTDC/MAR/100175/2008, Survivorship, long term movements, habitat
preferences and impacts of big game fishing on highly migratory sharks in the Algarve.
123.300 EUR, PTDC/MAR/104536/2008, Pelagic sharks in the eastern Atlantic Ocean: fisheries,
life history, migrations, population dynamics and demographics.
223
Fátima Abrantes (represented by João Coimbra)
CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental. Porto
The group which develops its activity at the Marine Geology Unit of LNEG was funded by several
sources: Ministry of Economy and Innovation (MEI), FCT (national projects and Eurocores ESF),
pluriannual funds through CIMAR and other funds for participation in meetings or short stays at
foreign laboratories.
The funds financially executed at CIIMAR in 2009:
-
-
National funding
172.800 EUR, FCT PTDC/CLI/70772/2006, INTERTRACE, 2008-2011. PI : A Voelker.
84.292 EUR, FCT PDCT/MAR/58282/2004, PORTO, 2006-2009. PI : A Voelker.
86.400 EUR, FCT PDCT/MAR/56963/2004, CUPEX, 2006-2010. PI : F Abrantes.
106.280 EUR, INTERREG IV POCTEP, Natura Miño-Minho, 2009-2010. PI : C Antunes.
175.163 EUR, FCT PTDC/MAR/65197/2006, MELT KP5 - Mantle melting and crust production in
the mid-atlantic ridge under the influence of Azores hotspot: a case study at KP-5 segment,
2008-2011.
224
1. Objectives
The general objectives of our group are the following: to execute the scientific projects that are
a part of our main research programme INGMAR - Investigação em Geologia Marinha with
excellent quality, submit and gain new projects, publish our results in high quality journals,
actively contribute to the education of new technicians and Marine geology Researchers, and,
disseminate the results obtained.
In scientific terms we plan to investigate the interaction between oceanic productivity, one of
the main mechanisms that control atmospheric pCO2 and climate, is one the main objectives of
the area of Paleoceanography / Paleoclimatology and Environment. As such we will concentrate
on the most recent periods of extreme climate, the Last Glacial maximum (LGM) and the
Optimum Holocene, and the older interglacial periods (Marine Isotopic Stage -MIS 5 a MIS 17).
Furthermore, since reconstructions can only be done on the basis of proxies, and their
utilization can only be done after calibration at the regional level, we will concentrate on the
calibration of planktonic foraminifera isotopes, by collecting alive forms at different depth of the
water column and do the isotope analyses on samples of both water and organism shells.
Another objective deals with the investigation of the origin of the different pollutants that reach
the coastal regions and their relation with climate, vegetation, agriculture techniques and type
of culture, that is, to evaluate the influence of human kind since 9,000 years B.P., through the
analyses of persistent organic pollutants, heavy metals and biomarkers in the sediments of the
Minho Estuary and the inner-shelf.
In the Framework of Geology and Resources, the geologic cartography (1/200.000) of the
Portuguese Continental Margin, including island of Madeira and Azores Archipelago and the
characterization of resources remain a main objective.
In terms of Coastal Geology, we plan to expand the actual research of the Troia península
(dunes and coastal line evolution) to Fonte da Telha and from Monte Gordo to Cacela a Velha in
the Algarve.
Within the theme of natural resources, we will continue the on-going research on the Mid
Atlantic Ridge sulfides, the Fe-Mn nodules and shelf inerts.
A calibration of sedimentary proxies to instrumentally measured data for the last 150 years was
published. This first attempt for this type calibration was done using sedimentary sequences
recovered from the inner shelf off the Tagus River mouth during a RV Poseidon campaign
organized by UGM.
The Holocene record of the Portuguese margin published by Teresa Rodrigues et al, constitutes
the highest resolution sedimentary record of the Holocene published to date. This fact has
allowed a detailed study of the sea level rise during the last transition, from the LGM to the
Holocene, and its consequences to the Tagus Estuary and inner shelf.
225
A comparison of the Sea Surface Temperature (SST) evolution during the Holocene and the
isotopic marine stage (MIS) 1, considered for a long time as the interglacial during which the
orbital conditions were the most similar to the present interglacial indicates that the Holocene is
comparable to MIS 11 1st half, independently of the orbital parameter used to align the
beginning of the two periods.
The study of the longest Pleistocene record of the Portuguese margin (750,000 years) reveals a
great similarity between the surficial oceanic conditions and the atmospheric conditions in
Antarctic (ice record). Besides, it also shows that the rapid climatic variability recorded in the
last glacial and transition periods are also observed in and before MIS 6. The same study has
also confirmed an intensification of the MOW during cold intervals at both orbital and millennial
scale.
A diatom record from the Bermuda Rise covering the 19,200 - 14,500 yr interval provides the
first detailed story of the primary productivity and circulation conditions during H1 and the
transition to the warm Bølling.
An Oregon (EUA) diatom record has allowed the datation of the great floods of the rocky
mountains, known to have occurred for a long time, but which age was unknown.
A study of a sedimentary record recovered from a levee of the Lisbon Canyon revealed a relation
between shelf and slope instability and the D-O stadials, with a 2,000 yr cycle.
Compilation of existing SST proxies for the Portuguese margiin allowed the drawing of SST maps
for specific time slices, incluindo the LGM. This has allowed us to define the Polar Front position
in a paper referred in Science as one of the editor’s choice.
Within the cooperation programme with other Portuguese speaking countries, our collaboration
with Mozambique and its national direction for geology “Direcção Nacional de Geologia de
Moçambique” (Cooperação IPAD/LNEG/DNG), lead to the completion of the Bazaruto Map
(1:25,000) and the initiation of the Bilene Map. During the fieldwork, several dune pulsations
and past coastlines were identified, what is an important contribution for the understanding of
the sea level variations and littoral evolution during the Pleistocene and Holocene.
In Tróia, the evolutive pattern of the peninsula was identified on the basis of the dunes
morphology and the internal structure of the sedimentation bodies. Dunes were dated by OSL in
order to establish a chronology for the peninsula evolution. To understand the rate of alteration
in recent times, volumes of erosion and accretion near the coast were estimated. Indication of
erosion possibly related to the 1755 tsunami was identified on the west side of the peninsula.
Submarine structures associated to the Sado delta were studied in order to investigate the
effect of sea level rise in the Sado area during the last transition.
The existing mineral resources data for the Portuguese margin was gathered and organized to
be included in the national POEM database.
At the Laboratory level, the biogeochemistry lab was the one where more new techniques have
been implemented: (1) the opal determination technique published by Mortlock and Froelich in
1989 is now optimized and running, (2) a microwave technique for dissolution of marine
226
sediment samples for Pb stable isotopes determination, (3) implementation of the method for
lipidic biomarkers by a modified Blye and Dyer technique in use at the biogeochemistry lab at
Marum, Bremen University, (4) implementation of the protocol for separation of lipid
biomarkers in fractions with increasing polarity using chromatography, again following the
method in use at the organic geochemistry lab at Marum, Bremen University.
8d. Productivity
1. Nair, G, Babu, DS, Damodaran, KT, Shankar, R, Prabhu, CN. 2009. Weathering of Ilmenite from
Chavara deposit and its comparison with Manavalakurichi placer ilmenite, southwestern India.
Journal of Asian Earth Sciences 34: 115-122. IF=1.842.
2. Lopes, C, Mix, AC. 2009. Pleistocene megafloods in the northeast Pacific. Geology 37(1): 7982. IF=4.368.
3. Tzedakis, PC, Pälike, H, Roucoux, KH, de Abreu, L. 2009. Atmospheric methane, southern
European vegetation and low-mid latitude links on orbital and millennial timescales. Earth and
Planetary Science Letters 27: 307-317. IF=4.062.
4. Mil-Homens, M, Branco, V, Lopes, C, Vale, C, Abrantes, F, Boer, W, Vicente, M. 2009. Using
factor analysis to characterise historical trends of trace metal contamination in a sediment core
from the Tagus Prodelta, Portugal. Water Air Soil Pollution 197: 277-287. IF=1.676.
5. Mil-Homens, M, Branco, V, Lopes, C, Vale, C, Boer, W, Alt-Epping, U, Abrantes, F, Vicente, M.
2009. Sedimentary record of anthropogenic metal inputs in the Tagus prodelta (Portugal).
Continental Shelf Research 29: 381-392. IF=2.183.
6. Voelker, AHL, de Abreu, L, Schönfeld, J, Erlenkeuser, H, Abrantes, F. 2009. Hydrographic
conditions along the Western Iberian Margin during Marine Isotope Stage 2. Geochemistry,
Geophysics and Geosystems 10, Q12U08. IF=2.626.
7. Lebreiro, SM, Voelker, AHL, Vizcaino, A, Abrantes, FG, Alt-Epping, U, Jung, S, Thouveny, N,
Gracia, E. 2009. Sediment instability on the Portuguese continental margin under abrupt glacial
climate changes (last 60 kyr). Quaternary Science Reviews 28: 3211-3223. IF=4.245.
8. Eynaud, F, de Abreu, L, Voelker, A, Schönfeld, J, Salgueiro, E, Turon, J-L, Penaud, A, Toucanne,
S, Naughton, F, Sanchez Goni, MF, Malaize, B, Cacho, I. 2009. Position of the Polar Front along
the western Iberian margin during key cold episodes of the last 45 ka. Geochemistry, Geophysics
and Geosystems 10: Q07U05. IF=2.626.
9. Stein, R, Hefter, J, Grützner, J, Voelker, A, Naafs, BDA. 2009. Variability of surface-water
characteristics and Heinrich-like Events in the Pleistocene mid-latitude North Atlantic Ocean:
Biomarker and XRD records from IODP Site U1313 (MIS 16 – 9). Paleoceanography 24: PA2203.
IF=3.644.
227
10. Bartels-Jonsdottir, HB, Voelker, AHL, Knudsen, KL, Abrantes, F. 2009. Twentieth century
warming and hydrographical changes in the Tagus Prodelta, Eastern North Atlantic. Holocene
19: 369-380. IF=2.481.
11. Gil, I, Keigwin, L, Abrantes, F. 2009. Deglacial diatom productivity and surface ocean
properties over the Bermuda Rise, northeast Sargasso Sea. Paleoceanography 24: PA4101.
IF=3.644.
12. Burbidge, C, Dias, M, Prudêncio, M, Rebelo, L, Cardoso, G, Brito, P. 2009. Internal alpha
activity: localization, compositional associations and effects on OSL signals in quartz approaching
beta saturation. Radiation Measurements 44: 494-500. IF=0.973.
13. Costas, S, Muñoz Sobrino, C, Alejo, I, Pérez-Arlucea, M. 2009. Holocene evolution of a rockbounded barrier-lagoon system, Cíes Islands, NW Iberia. Earth Surface Processes and Landforms
34: 1575-1586. IF=2.055.
14. Gherardi J-M, Labeyrie, L, Nave, S, Francois, R, McManus, JF, Cortijo, E. 2009. Glacialinterglacial circulation changes inferred from 231 Pa/ 230 The sedimentary record in the North
Atlantic region. Paleoceanography 24: PA2204. IF=3.644.
15. Abrantes, F, Lopes, C, Rodrigues, T, Gil, I, Witt, L, Grimalt, J, Harris, I. 2009. Proxy calibration
to instrumental data set: Implications for paleoceanographic reconstructions. Geochemistry,
Geophysics and Geosystems 10: Q09U07. IF=2.626.
16. Freitas, PS, Clarke, LJ, Kennedy, H, Richardson, CA. 2009. Ion microprobe assessment of the
heterogeneity of Mg/Ca, Sr/Ca and Mn/Ca ratios in Pecten maximus and Mytilus edulis (bivalvia)
shell calcite precipitated at constant temperature. Biogeosciences 6(7): 1209-1227. IF=3.246.
17. Roger Searle, Bramley Murton and the JC24 Shipboard Scientific Party (Kay Achenbach, P.L.
Ferreira, Tim LeBas, Chris Mallows, Kirsty Morris, Nicole Schroth, Maurice Tivey, Peter van
Calsteren , Chris Waters and Isobel Yeo). 2009. Structure and development of the axial volcanic
ridge: cruise JC24 to the Mid-Atlantic Ridge, 45ºN. InterRidge News 17: 11-15.
18. Naughton, F, Sánchez Goñi, MF, Kageyama, M, Bard, E, Cortijo, E, Desprat, S, Duprat, J,
Malaizé, B, Joli, C, Rostek, F, Turon, J-L. 2009. Wet to dry climatic trend in north western Iberia
within Heinrich events. Earth and Planetary Science Letters 284: 329-342. IF=4.062.
19. Rodrigues, T, Grimalt, JO, Abrantes, F, Flores, JÁ, Lebreiro, S. 2009. Interdependences of
changes in sea surface temperature, productivity and fluvial inputs in the continental shelf of
the Iberian margin (Tagus mud patch) during the Holocene. Geochemistry Geophysics
Geosystems 10: Q07U06. IF=2.626.
20. Voelker, A, Rodrigues, T, Stein, R, Billups, K, Oppo, D, McManus, J, Hefter, J, Grimalt, JO.
2009. Variations in mid-latitude North Atlantic surface water properties during the mid-Brunhes:
Does Marine Isotope Stage 11 stand out? Climate of the Past 5(3): 1553-1607. IF=3.826.
228
In press
21. Salgueiro, E, Voelker, A, de Abreu, L, Abrantes, F, Meggers, H, Wefer, G. (in press).
Temperature and Productivity Changes off the Western Iberian Margin during the last 150 ky.
Quaternary Science Reviews. IF=4.245.
proceedings)
1. Abrantes, F, Lopes, C, Matos, L. 2009. Médium Si and diatom preservation potential in
sediments. European Geosciences Union General Assembly, Austria. Geophysical Research
Abstracts, Vol. 11, EGU2009-3689,
2. Abrantes, F, Rodrigues, T, Montanari, B, Santos, C, Voelker, A, Lopes, C. 2009. Precipitation
maxima and upwelling trends at the NAO Southern Pole during the Last Millenium. European
Geosciences Union General Assembly, Austria. Geophysical Research Abstracts, Vol. 11,
EGU2009-3689.
3. Costas, S, FitzGerald, D. 2009. Holocene evolution of the Southern-end of Salisbury Beach,
MA: sea-level rise and tidal inlet forcings. Northeastern Section, 44th Annual Meeting Geological
Society of America Abstracts with Programs 41(3): 85.
4. Rebelo, L, Ferraz, M, Brito, P. 2009. Tróia Peninsula Evolution: The dune morphology record.
Proceedings of the 10th International Coastal Symposium, Journal of Coastal Research, SI 56:
352-355.
5. Rodrigues, T, Voelker, A, Grimalt, JO, Abrantes, FF. 2009. Climate off Portugal during Marine
Isotope Stages 15 - 9 (570 to 300 ka): Suborbital glacial variability and interglacial stability.
American Geophysical Union Fall Meeting, USA. Abstract PP31C-1370.
6. Kulhanek, DK, Voelker, AHL, Gruetzner, J. 2009. Surface water hydrography in the mid-latitude
North Atlantic (IODP Site U1313) from 480-355 ka: observations from calcareous
nannoplankton. American Geophysical Union Fall Meeting, USA. Abstract PP31A-1290.
7. Patton, G, Martin, PA, Voelker, A. 2009. Trace and minor element/Ca ratios of planktonic
foraminifera in the Iberian Margin during Heinrich events. American Geophysical Union Fall
Meeting, USA. Abstract PP31B-1327. American Geophysical Union Fall Meeting, USA. Abstract
PP14C-01.
8. Salgueiro, E, Martin, PA, Voelker, A, Abrantes, FF. 2009. Temperature calibration along the
Iberian–NW Africa margin, using modern planktonic foraminifera trace element and stable
isotope ratios. American Geophysical Union Fall Meeting, USA. Abstract PP31B-1334.
9. Voelker, AHL, Lebreiro, S. 2009. Deep Water properties in the North Atlantic's eastern and
western basins during the Mid-Brunhes (330-630 ka). European Geosciences Union General
Assembly, Austria. Geophysical Research Abstracts, Vol. 11, EGU2009-5782.
229
proceedings)
MASTER THESES
Rebotim, AS. 2009. Foraminíferos planctónicos como indicadores das massas de água a norte e a
sul da Frente/Corrente dos Açores: Evidências de dados de abundância e isótopos estáveis.
Master thesis in Sciences of the Sea - Sea Resources, ICBAS, University of Porto. Supervisor: A
Voelker.
PhD THESES
Brito, P. 2009. Impactos da elevação do nível médio do mar em ambientes costeiros: O caso do
Estuário do Sado. PhD thesis in Geology, University of Lisbon. Supervisor: F Abrantes.
Rodrigues, T. 2009. Reconstrução das condições Climáticas na Margem Portuguesa durante o
Holocénico - Analogias com o Estádio 11. PhD thesis, Cataluña Tecnical Universitat, Spain.
Supervisor: F Abrantes.
-
Integrated Ocean Drilling Program (ICDP) COUNCIL Meeting, 22-23 January, Lisbon,
Portugal.
-
Integrated Ocean Drilling Program (IODP) Science Advisory Structure Executive
Committee (SASEC) Meeting, 20-21 January, Lisbon, Portugal.
-
Integrated Ocean Drilling Program (IODP) Science Support and Advisory Commitee of
ECORD (ESSAC), 26-27 May, Sesimbra, Portugal.
-
4th MedCLIVAR Workshop, 28-30 September, Sesimbra, Portugal.
-
European Science Foundation Magellan Workshop: The Shackleton Sites, 9-10
November, Lisbon, Portugal.
230
Visits / Training at Foreign Laboratories
-
Univ Chicago, Dept Geophysical Sciences, Chicago, USA, 6 June - 30 August, research
stay related to INTER-TRACE project (AVoelker).
-
GEOTOP, UQAM, Montreal, Canada, 31 August-3 Sept; related to INTER-TRACE project
(A Voelker).
-
United States Geological Survey (USGS), Menlo Park, Califórnia with Robert Kayen
(USGS), 24 April-19 June, Luso-American Foundation (FLAD) fellowship Luiz
Saldanha/Ken Tenore - 2008 - Ecologia das Interfaces Terra-Mar (MFerraz).
-
Vigo Univ, 29 Nov-6 Dec, with Mariano Lastra and Eduardo Jaramillo professor of the
Universidad Austral del Chile (M Rufino).
-
LSCE (Gif-sur-Yvette) results discussion and strategy definition for future work and subsampling of cores under study by the bilateral GRICES/CNRS and AMOCINT projects; 1819 May and 02-04 December (S Nave, L Matos, A Alberto).
-
GEOMAR, 21 April-15 May, 1 June-31 July, 3 Nov-1 Dec (S Muiños).
-
GEOMAR (M Nuzzo).
-
MARUM, Bremen University (M Nuzzo).
-
WOODS-HOLE Oceanographic Institute, May-July (I Gil).
Participation in Cruises
-
POS303 Oceanographic cruise, on board R/V Poseidon between Ponta Delgada and
Funchal. 20 April-4 May2009. Water and plankton sampling. Participation of A Rebotim.
-
Oceanographic campaign MINHO09 in Rio Minho. 10-15 August 2009.
-
M79-2 Oceanographic cruise, on board R/V Meteor between S. Miguel, Açores and
Lisbon to study the Glória Fault. 26 August-21 September 2009, PI Christian Hübscher.
Participation of L Batista.
-
Oceanographic cruise GEOSTAR09. 31 November-12 December. Participation of R
Borges.
-
IODP Leg 323 Bering Sea, RV Glomar Challenger, July-September 2009. Participation of Z
Stroynowski.
231
Cooperation with Mozambique
-
Geologic and Geoenvironmental Map of the Bilene Coastal Region, scale 1:50000, SubProject “Cartografia Geológica da Região Costeira do Bilene. scale 1:50000”.
Reviews for ISI Journals: 21 journals, 32 reviews
Review for Funding Agencies: NSF / USA (3), ESF / Europe (3)
International Committees Participation:
-
PAGES Science Steering Committee (F Abrantes).
-
IODP-ESSAC (F Abrantes).
-
ESF Mg Workshops Programme Steering and Science Committees (F Abrantes).
-
ESF PolarCLIMATE Panel (F Abrantes).
-
ESF MedCLIVAR Programme Steering and Scientific Committees (F Abrantes).
-
Eurogeosurveys Marine Group (F Abrantes).
-
IMAGES (A Voelker).
-
InterRidge Steering Committee (P Ferreira).
GPO Group participates in NATURA-MINHO-MIÑO, a project funded by Interreg IV POCTEP, the
Transboundary territorial cooperation program between North of Portugal and Galicia. This
project aims the join valorization of natural resources in the river Minho Hydrographical basin,
the promotion of sustainable development and biodiversity and habitat conservation (Natura
2000) in the transboundary territory of North of Portugal and Galicia. It involves the
participation of regional authorities (General Directorate of Nature Conservation in Galicia and
ICBN - Institute for Nature Conservation and Biodiversity of the Portuguese Ministry for the
Environment) assuring the effective involvement in public policy advice.
8e. Future research
1. Objectives
All the funded projects are part of INGMAR, the main research programme of the UGM/LNEG,
and represent three of its five main areas of activity Paleoceanography/Paleoclimatology and
Environment, Coastal Geology and Mineral Resources.
232
Within the Paleoceanography/Paleoclimatology and Environment, the ongoing and near future
research can be clustered around three main themes:
-
New Proxies Development
-
Transferring Microfossil and Biochemical Proxies into Quantitative Climate Variables
-
Application of Known Existing Techniques / Methods and Calibrated Proxies to Regional
Archives for High Resolution Climate and Productivity Reconstructions at Target Periods
(Transition Periods, Extreme and/or rapid climatic events, past natural warm states)
Following the recommendations of the National Research Council report on “Abrupt Climate
Change” (National Research Council-US, 2002), as well as the IMAGES and the IGBP /PAGES
programmes, concerning the need for a good knowledge of the climate changes in climatically
sensitive regions of the world’s ocean at times of extreme climate conditions we have
concentrated on the reconstruction of the last climatic extremes, i.e. the last glacial maximum
(LGM) and the Holocene optimum (HO) in the five most important upwelling areas of the globe
(CUPEX).
The study of the HOLOCENE, our present interglacial is considered of great importance and is
the period of concern in the projects (CLIMHOL and MONA)
Furthermore, we have been working on the reconstruction of the Holocene climate, the warm
period in which we live, as well as of previous interglacials such as the Marine Isotopic Stage
(MIS) 11, the most recent warm period with orbital (eccentricity) conditions similar to the
Holocene. These two warm periods may constitute an important source of information for
future climate modeling and their comparison could allow an estimation of the human
contribution to a naturally variable climatic cycle (PORTO).
Moreover, we have also been working on proxy calibration at the regional level, always
necessary to better understand past climate variability forcing processes and potentially extract
seasonal signals (INTERTRACE, CALIBERIA).
In terms of environment, our research centers on the distribution and historical evolution of
polluting elements in the Portuguese estuaries and shelf environments (NATURE MINHO-MIÑO).
Other theme recently embraced is Climate Change and Human Societies / Culture and
Organization (CLIMIBERIA).
In which respects Mineral Resources, we are mainly concentrating on Agregates and Mid
Atlantic Ridge (MAR) Sulfides.
Regarding aggregates we are trying to understand, based on field data and supported by
modeling, the role of volcanism, sedimentation, wave erosion and relative sea-level changes in
the evolution of young volcanic island shelves, using Faial Island as a case-study. The aim is to
comprehend how these different processes acting on the Azores island shelves contributes to
the development of sand and gravel deposits (PLATAF). In terms of Sulfides, besides the interest
in identifying the different mineralization-types formed along the non-sedimentary Mid.Ocean
Ridge, another objective is to establish a model of crustal accretion in the MAR.
233
Coastal Geology Research will be mainly focused in understanding, at different time scales, the
evolution of the coastal regions, in particular sandy beach and dune areas. Coastal ecology in
sandy beach environments and storm events consequences in the ecosystems are also being
studied since this relationship may be a valuable tool to evaluate and predict the effect of global
change in sandy beaches fauna.

Current funding
172.800 EUR, FCT PTDC/CLI/70772/2006, INTERTRACE, 2008-2011. PI : A Voelker.
84.292 EUR, FCT PDCT/MAR/58282/2004, PORTO, 2006-2009. PI : A Voelker.
86.400 EUR, FCT PDCT/MAR/56963/2004, CUPEX, 2006-2010. PI : F Abrantes.
106.280 EUR, INTERREG IV POCTEP, Natura Miño-Minho, 2009-2010. PI : C Antunes.
175.163 EUR, FCT PTDC/MAR/65197/2006, MELT KP5, 2008-2011.
FCT PTDC/MAR/102045/2008, CALIBERIA - Multi-proxy Calibration along the NW Iberian margin:
Improving paleoceanographic reconstructions. 2010-2012. PI: E Salgueiro.
FCT PTDC/AAC-AMB/108449/2008, MONA - Integration of proxy data from the North Atlantic
for modelling climatic events. 2010-2012. PI: I Gil.
FCT PTDC/AAC-CLI/100157/2008, CLIMHOL - Holocene climatic variability in the North Atlantic
and adjacent landmasses: land-sea direct correlation. 2010-2012. PI: F Naughton.

Pending funding (submitted to FCT)
PLATAZ - Processos construtivos e destrutivos na evolução de plataformas insulares vulcânicas :
As ilhas dos Açores. IP : R Quartau.
CLIMIBERIA - Climate influence on Norwestern Iberian society during the late Holocene. PI : CN
Prahu.
BioPraia - Sandy beaches in a changing environment : effects of tides and storms in a global
change context.
234
Hydrobiology
Hydrobiology
Adriano Agostinho Donas Bôto Bordalo e Sá
Total funding in 2009: ca 350.000 EUR.
-
-
National funding.
2.800 EUR, FCT PTDC/AMB/64441/2006.
30.000 EUR, FCT ERA-AMPERA/0003/2007, OILDEBEACH.
23.000 EUR, Annual research funding ICBAS-UP.
INAG, POVT-03-0133-FCOES-00017, Avaliação do estado ecológico das massas de água costeiras
e de transição e do potencial ecológico das massas de água fortemente modificadas. 2009-2011.
Confidential budget.
35.000 EUR, QREN-2009/003442- ECOPISCIS, 2009-2011.
Aida, Spain - Seasonal variability of water quality in the new water network of Bolama, Bijagos
archipelago; Assessment of water quality in wells in the Buba area, Guinea-Bissau. Confidential
budget.
Spanish Red Cross - Assessment of water quality in wells and boreholes in the Gabu, Bafatá and
Bubaque areas, Guinea-Bissau. Confidential budget.
Suldouro, Portugal - Avaliação do potencial de eutrofização do Rio Inha na envolvente da exlixeira de Canedo, 2009-2010. Confidential budget.
AHR Norte, Portugal - River Douro estuary management plan. Development of the model for
tenders call, 2009-2010. Confidential budget.
235
-
FCT Scholarships
8.250 EUR, SFRH/BD/22317/2005, SFRH/BD/47631/2008, SFRH/BD/46146/2008.
-
Others
4.000 EUR, Univ Porto - Microbial Life in Extreme Antarctica Environments.
500 EUR, Univ Porto. Escola de Ciências da Vida e da Saúde. Summer course.
1. Objectives
The activities within the lab, in what CIIMAR is concerned, are spread over three main areas: (i)
research; (ii) consulting; and (iii) outreach. The lab aims to contribute to the advance of science
from a multidisciplinary point of view, dealing from water quality monitoring to modelling, from
early life stages of fish to denitrification and global warming and environmental and human
health. Research is carried out in temperate (Portugal), tropical (Thailand - SE Asia, GuineaBissau - W Africa, S. Tomé and Principe - W Africa) and extreme environments (Antarctica). The
main research areas are (a) monitoring estuarine water quality in terms of physical
(conductivity, salinity, temperature, turbidity), chemical (dissolved oxygen, inorganic carbon, pH,
nitrate, nitrite, ammonium, phosphate, silicate) and biological (bacteria, viruses, fecal indicators,
primary production) parameters, (b) estuarine primary productivity, (c) nitrogen and sulfur
cycles; processes, interactions and microbial communities implicated (d) effects of N and S
cycles disturbances on cumulative global warming potential (e) ecology of microbial
communities in the extreme cold environments of Antarctic ice-free Dry Valeys, with a focus on
the main N cycle microbial contributors (f) anthropogenic impact in estuarine communities, (g)
phytoremediation and bioremediation, highlighting the role of the both plants and
microorganisms in the removal of metals and hydrocarbons from estuarine and coastal
sediments, (h) estuarine fish assemblages, with special emphasis to the estuarine nursery role
for early life stages of fishes (i) implementation of the Water Framework Directive (WFD), (j)
ecological modelling, (k) tropical water quality, (j) ecology of cholera, (h) water management
and public health.
Monitoring estuarine water quality
-
Research in the River Douro estuary involving monthly water column (since 1985) key
environmental data.
Estuarine primary productivity
-
236
Research in the River Douro estuary has been performed in the water column, intertidal
sediments and hard surfaces.
Nitrogen cycle and global warming
-
Research has been carried out in the water, sediments and hard surfaces in several
estuaries in Portugal as well as in Thailand. Nitrogen fixation, nitrification,
denitrification, annamox and nitrous oxide production, a powerful green-house gas
were evaluated. In addition, the structure of the microbial populations involved in
nitrification and denitrification and anammox are also studied.
Nitrogen and Sulfur Cycle interactions
-
Discovery of the occurrence of a novel inhibitory interaction between organic sulfur
degradation compounds and the last step of denitrification (i.e. reduction of N2O and
N2) in estuarine sediments and in a bacterial pure culture (Silicibacter pomeroyi). The
inhibition of the last step of denitrification limits nitrogen loss through N2 and enhance
nitrogen loss via N2O, a greenhouse gas. These findings represent a new perspective
concerning the interaction and controls between nitrogen and sulfur cycles in the
marine environment and promote a new line of research that is being currently
investigated.
Ecology of Microbial communities in Antarctic Soils
-
Research on bacteria cyanobacteria and ammonia oxidizers biodiversity and response to
environmental constraints has been carried out in the ice-free regions of Transantarctic
Mountains. A multidisciplinary approach to understand how geological history,
geographic gradients, and soil geochemical variables drive spatial differences in diversity
and distribution of the microbial communities was used.
Anthropogenic impact on estuarine communities
-
Research performed in estuaries in order to understand the effect of heavy metals and
other pollutants on macrobenthic and microbial communities and on the denitrification
process with a main focus on N2O emissions.
Phytoremediation and bioremediation
-
The role of the interaction between plants and microorganisms in the removal of heavy
metals and organic pollutants from sediments has been studied in several estuaries in
Portugal (Douro, Cávado and Lima). Additionally, the feasibility of bioremediation as a
clean-up tool of subsurface oil has been evaluated, by studying microbial communities,
and their hydrocarbon degradation potential, in beaches that still contaminated several
years after the sinking of the Prestige oil tanker (N.W. Spain).
Estuarine fish assemblages
-
Understanding of fish dynamics, focusing on the potential nursery role of estuaries and
ultimately assess the carrying capacity of these ecosystems for fish species, some with
high economical important such as sardines and flatfishes. Deriving and testing of an
ecological indicator of the fish estuarine nursery function, to evaluate the ecological
237
status of estuarine ecosystems, towards the implementation of the Water Framework
Directive.
Implementation of the WFD
-
Research conducted in several northern Portuguese estuaries in order to assess the
ecological status of transitional waters and develop tools towards the implementation
of the Water Framework Directive.
Ecological modeling
-
By means of an object-oriented model several key parameters, including primary
production, have been modelled in the River Douro estuary.
Tropical water quality, cholera and public health
-
Research in Thailand, Guinea-Bissau and S. Tomé & Principe. In Thailand the water
quality of the Bangpakong River watershed and subtidal sediments has been carried out.
In Guinea-Bissau, water quality and its relation to disease including cholera has been
evaluated and sustainable measures do increase water quality have been designed. In S.
Tomé & Principe the water quality in the island of S. Tomé was evaluated for the first
time.
Outreach
-
Several activities fostering the dialogue between scientists and society, to improve the
public knowledge and perception of science and to increase the interest of the young in
science, namely through participation in the summer courses of University of Porto
(Universidade Junior) and collaboration with Ciência Viva (Projecto Oceanos,
Biodiversidade e Saúde Humana) will be implemented. Moreover, scientific sound
measures to reduce the risk of using contaminated water to be implemented in a
sustainable way in Wets Africa will be developed.
8d. Productivity
Estuarine Coastal and Shelf Science 85: 627-632. IF=1.970.
238
3. Magalhães, C, Machado, A, Bordalo, AA. 2009. Temporal variability of relative abundance of
ammonia oxidizing bacteria vs archaea in the sandy flat of the Douro River estuary, Portugal.
Aquatic Microbial Ecology 56: 13-23. IF=1.743.
4. Mesquita, R, Ferreira, T, Segundo, R, Teixeira, C, Bordalo, AA, Rangel, AOSS. 2009.
Development of a sequential injection system for the determination of nitrite and nitrate in
waters with different salinity: application to estuaries in NW Portugal. Analytical Methods 1:
195-202.
5. Ramos, S, Ré, P, Bordalo, AA. 2009. New insights into the early life ecology of Sardina
pilchardus (Walbaum, 1792) in the Northern Atlantic. Scientia Marina 73: 449-459. IF=1.174.
6. Ramos, S, Ré, P, Bordalo, AA. 2009. Environmental control on early life stages of flatfishes
within a nursery area (Lima estuary, NW Portugal). Estuarine Coastal and Shelf Science 83: 252264. IF=1.970.
proceedings)
proceedings)
PhD THESES
Carvalho, L. 2009. Metodologias para a avaliação integrada dos impactos cumulativos em
Aquatic Sciences, ICBAS, University of Porto. Supervisor: A Bordalo e Sá, Co-Supervisors: Rui
Cortes (UTAD) and Joaquim Poças Martins (Faculty of Engineering, University of Porto).
-
River Douro Estuary Management Plan Workshop, 23 November, Porto, Portugal.
239
Aida, Spain - Seasonal variability of water quality in the new water network of Bolama, Bijagos
archipelago, Guinea-Bissau.
Aida, Spain - Assessment of water quality in wells in the Buba area, Guinea-Bissau.
Spanish Red Cross, Spain - Assessment of water quality in wells and boreholes in the Gabu,
Bafatá and Bubaque areas, Guinea-Bissau.
Suldouro, Valorização e Tratamento de Resíduos Sólidos Urbanos, SA (Portugal) - Avaliação do
potencial de eutrofização do Rio Inha na envolvente da ex-lixeira de Canedo.
Northern River Basin Administration (ARH-Portugal) - River Douro estuary management plan.
Development of the model for tenders call.
United Kingdom
-
IECS, U. Hull: Prof. M. Elliott. Derive and test a quantitative indicator of ecosystem
functioning and health for use in management plans and the future policies for the
estuarine management.
Spain
-
GEOMA, University of Vigo. Prof. Ana Bernabeu. Buried oil in the intertidal beach zone:
coupling between beach morphodynamic, natural degradation, forcing mechanisms and
biological activity.
France
-
GLADYS, University of Montpellier 2. Prof. Frédéric Bouchette. Buried oil in the intertidal
beach zone: coupling between beach morphodynamic, natural degradation, forcing
mechanisms and biological activity.
United States of America
-
Dep. of Marine Science, UGA: Prof. W Wiebe, Prof. J Hollibaugh, Prof. S Joye. Structure
and function of microbial communities; biogeochemical cycling of nutrients.
-
RSMAS, U. Miami: Prof. R Cowen and Prof. C Paris, Fisheries oceanography and larval
ecology.
-
Dauphin Island Sea Lab, University of South Alabama. Prof. R Kiene. Cycling of organic
sulfur in aquatic systems.
-
Dep. of Microbiology, University of Tennessee. Prof. A Buchan. Ecology of microbial
communities that mediate biogeochemical processes of organic sulfur cycling.
240
-
University of Maryland. Prof. Rita Colwell. Cholera dynamics in aquatic systems.
New Zealand
-
U. Waikato. Prof. C Cary, Prof. I R McDonald. Microbial diversity and environmental
constrains along latitudinal gradient of Antarctic terrestrial environments.
Brasil
-
LIZI, Universidade Federal do Rio de Janeiro: Prof. Ana Bonecker. Ichthyoplankton
biology and ecology in tropical and temperate estuaries.
Guinea-Bissau
-
Variability of well/borehole water quality dry/wet seasons, outbreak of intestinal
diseases and cholera. AIDA (NGO Ayuda, Intercambio y Desarrollo) and Spanish Red
Cross.
QREN- 2009/003442: ECOPISCIS. 2009-2011.
POVT-03-0133-FCOES-00017- INAG: EEMA- Avaliação do Estado Ecológico das Massas de Água
Costeiras e de Transição e do Potencial Ecológico das Massas de Água Fortemente Modificadas.
2009-2011.
Suldouro, Valorização e Tratamento de Resíduos Sólidos Urbanos, SA: Avaliação do potencial de
eutrofização do Rio Inha na envolvente da ex-lixeira de Canedo. 2009-2010.
Seasonal variability of water quality in the new water network of Bolama, Bijagos archipelago,
Guinea-Bissau. Aida, Spain. 2008-211.
Assessment of water quality in wells in the Buba area, Guinea-Bissau. Aida, Spain. 2010-2011.
Assessment of water quality in wells and boreholes in the Gabu, Bafatá and Bubaque areas,
Guinea-Bissau. Spanish Red Cross. 2009-2010.
241
8e. Future research
1. Objectives
The lab deals with a wide range of ecological research challenges with special emphasis to:
estuarine and coastal dynamics, watershed management, Water Framework Directive
implementation, microbial diversity and function, nitrogen and sulphur cycles and global
warming, bioremediation, tropical water quality and public health.
Since 1985, research has been focused on the Douro estuary and the coastal zone of Porto.
Progressively, expanded into other Portuguese estuaries (Lima, Cavado, Ave, Leça) and other
continents (SE Asia, West Africa). Besides basic knowledge of each system, the attention of the
researchers has been devoted to processes and functions and their implications to the health of
the ecosystems, human health included.
The current management of aquatic ecosystems relies on a good knowledge of their structure
and functioning and of the way in which the habitat status, use, loss and gain has been
influenced by human activities. The EU Water Framework Directive requires the classification of
the ecological status of all water bodies. Thus, research in this area will deal with development
of tools that allow the assessment of ecological status of these ecosystems, the evaluation of its
response to natural and man-induced stressors and the design of mitigation measures to fulfill
legislation requirements. Those tools will include the design of conceptual models of ecosystems
function, development of new environmental indicators and methodologies for health
assessment and restoration of small watersheds.
Besides the evaluation of microbial water quality in estuarine, fluvial and well water, particular
attention is devoted to the microbial diversity of populations inhabiting those environments
related to disease, such as the cholera vibrion.
The evaluation of new interactions between organic sulfur degradation compounds and the last
step of denitrification, including processes (involved in global warming) and microbial
communities is an ongoing research. These studies represent a new perspective of the
interaction between marine nitrogen and sulfur cycles, giving a new understanding about
nitrous oxide production in the marine environment. Firstly, the identification and
characterization of the specific organic sulfur agent(s) responsible for the inhibition of the last
step of denitrification will be carried out and secondly the evaluation of the environmental
significance and implications of these inhibitory processes in estuarine systems will be carried
out.
Ongoing research on the nitrogen cycle will deal with the role of denitrification, anammox,
dissimilatory nitrate reduction to ammonium and nitrification as fates for nitrogen
transformations in estuarine sediments. The dynamics of microbial communities and of key
nitrogen processes with main relevance to potential release of N2O (with high impact in global
warming), in tropical estuaries will also be addressed.
Research will be extended to Antarctica terrestrial environments by investigating the
distribution, phylogenetic affinities and aspects of the ecology of different microbial
communities in a high range of Antarctic soil environments (Transantarctic Mountains) and
242
identify key physical and chemical or geologic variables as controls for the patterns of diversity,
abundance and distribution of microbial communities that inhabit these extreme environments.
Phytoremediation and bioremediation are approaches potentially less damaging and cost
effective for the recovery of contaminated estuarine and coastal areas when compared to
alternatives such as soil/sediment washing, incineration or disposal to landfill. Thus, the
research presently carried out will be continued in order to investigate potential plantmicroorganisms associations that could, on one hand, improve metal phytoremediation
capacities of the plant and, on the other hand, improve bioremediation of organic compounds,
namely PAHs, by microorganisms in estuarine salt marshes. Another research field is the
development of methodologies for the cleanup of subsurface oil contaminated sediments in
coastal beaches, based on the stimulation of the degradation processes of microbial
communities within the sedimentary column. These also involve the isolation of autochthonic
hydrocarbon biodegrading microorganism, not genetically modified.
Tropical research in West Africa (Guinea-Bissau) will be enlarged, dealing with well and borehole
water quality (the sole water source in the country for >90% of the population in the continental
and the insular areas of the country), aiming the design and implementation of simple and
sustainable measures to decrease the burden associated to the consumption of contaminated
water. Also, the dynamics and controls for the survival of Vibrio cholerae, responsible for the
recurrent epidemics of cholera that plague the country, and the link between water quality and
human health and the iodine deficiency that affects 90% of Guinean children and is responsible
for slow mental development will be addressed.

Current funding
25.800 EUR, FCT PTDC/AMB/64441/2006, AQUASENSE, 2008-2010.
35.000 EUR, QREN- 2009/003442, ECOPISCIS, 2009-2011.
30.000 EUR, ERA-AMPERA/0003/2007, OILDEBEACH, 2008-2011.
4.000 EUR, Pluridisciplinary projects program of University of Porto - Microbial Life in Extreme
Antarctica Environments, 2009-2010.
INAG, POVT-03-0133-FCOES-00017- EEMA, 2009-2011.
130.000 EUR, FCT PTDC/AAC-CLI/103539/2008, CHOLERA - Towards the understanding of Vibrio
cholerae dynamics and controls in tropical African wells (Guinea-Bissau), 2010-2013.
115.906 EUR, FCT PTDC/MAR/098914/2008, NITROSUL - Novel interaction between marine
biogeochemical nitrogen and sulfur cycles: characterization and ecological implications, 20102013.
243
125.256 EUR, FCT PTDC/MAR/099140/2008, PHYTOBIO - Phytoremediation and bioremediation
of contaminants in salt marshes: plant-microorganisms interactions, 2010-2013.
Aida, Spain, 2010-211, Confidential budget.
Spanish Red Cross, 2010, Confidential budget.
Implementation of River Douro estuary management plan, Northern River Basin Administration,
Portugal, 2010-2011.
25.000 EUR, Annual research funding ICBAS-UP, 2010.

Pending funding
135.264 EUR, FCT PTDC/AAC-AMB/113973/2009, NITROTOX – Interference of Metals and PAHs
in Nitrate Removal Biological Processes: Denitrification vs Anammox.
112.368 EUR, FCT PTDC/MAR/112723/2009, AMOX - Relative importance of archaeal and
bacterial ammonia oxidizers in estuarine ecosystem function: role of environmental controls.
125.460 EUR, FCT PTDC/MAR/113134/2009, EcoEarlFish - Early life stages of fishes as indicator
of the ecological status of estuarine nursery function.
244
Marine Biotechnologies (MarBiotech)
Biotechnology and Molecular Biology of Microalgae (BMBM) now consolidated as Marine
Biotechnologies (MarBiotech)
João Carlos Serafim Varela
Funding in 2009:
-
-
National funding
6.000 EUR, Agência de Inovação, NITEC-GENTEC 161/26/06, Stimulating systems for the creation
of research & technological development nuclei in entrepreneurial sector. 2007-2009. Team
member.
1. Objectives
Up to 2008, the main research field of the MarBiotech (formerly known as BMBM:
Biotechnology and Molecular Biology of Microalgae) research group was focused on the
elucidation of the molecular basis of the significant carotenoid accumulation in the microalga
Dunaliella salina. This coincided with the defense of two PhD theses and the publication of five
articles on this very subject in 2008-2009. However, as the marine biotechnology scientific field
at large has become quite dynamic, the Principal Investigator decided to widen the scientific
objectives of the group, extending its research interests over to three emerging research topics:
1) microalgae-derived biofuels; 2) marine bioactive compounds; and 3) iron homeostasis in
cyanobacteria.
Line Research 2) is essential for the implementation of a bio-refinery strategy for the production
of High Valuable Products (HVPs). HVPs will be used to defray production costs of the biomass
and enable entrepreneurial partners to produce biofuels (mainly biodiesel and bio-jetfuel) at
245
competitive prices with petrodiesel. Iron homeostasis is a recent line of research due to a
collaborative effort of MarBiotech with Dr. Matthias Futschik’s lab at CBME (Univ. Algarve) and
Prof. Wolfgang Hess’s lab (Univ. Freiburg, Germany) using a systems biology approach to
understanding how iron availability is regulated in ecologically relevant cyanobacteria.
In 2009, two new Post-Doctoral researchers were attracted to the MarBiotech research group to
implement two novel research lines, namely Luísa Barreira (LB), an experienced analytical
chemist and Luísa Custódio (LC), a post-doctoral fellow with large experience in detecting
natural products via bioassays. We also attracted a graduate student, Hugo Pereira, who had
worked at our entrepreneurial partner (Necton, S.A.) for the optimization of growth media for
large-scale microalgal biomass production. HP has recently earned an MSc degree on
Aquaculture and Fisheries applied to microalgae-derived biofuel production. In collaboration
with CCMAR Aquagroup LB and HP have determined the lipid profile of microalgal strains grown
at Necton S.A. Nannochloropsis oculata and a Chlorella spp., the latter a fast-growing
autochthonous isolate. Our results indicate that the N. oculata strain has a very high
polyunsaturated fatty acid (PUFA) composition that may render it unsuitable for biofuel
production when grown in “green walls”. However, the autochthonous strain was shown to
contain significantly less PUFAs and a profile suitable for the production of bio-jetfuel. We have
presented these results to the executive board of a Necton S.A. spin-off (AlgaFuel S.A.) and we
are awaiting their decision concerning future developments. In 2009, we have been contacted
by the Portuguese fish farming company (AtlantikFish S.A.) in order to develop suitable
microalgal candidates for biofuel production. This company has a 50-acre area of ponds that are
ready to be used for large-scale production of microalgae-derived biofuel with only minor
adaptations. At different time-points throughout the year, we have collected several samples
from their ponds to isolate autochthonous microalgal strains already adapted to the local
conditions of salinity, temperature and light regimes. We are currently investigating their
potential for biofuel production, namely: i) growth rates, ii) suitable light, nutrient, salinity and
temperature regimes in order to induce lipid biosynthesis, as well as iii) lipid profiles of
microalgae under control and lipidogenic conditions. In order to engage undergraduate students
in this effort, one student (Ana Filipa Sobral) joined MarBiotech; this student has earned a BII
grant from FCT in order to study at least one promising autochthonous microalgal strain and its
potential for biofuel production in AtlantikFish ponds.
In the last half of 2009, LC and LB, gathered 22 different macroalgae species, 2 seagrasses and 4
salt marsh plants at different locations on the Algarve coast. In addition, 11 microalgae species
were obtained from Necton, SA and from the LEOA at the University of Algarve. Natural
compounds were extracted from these samples using solvents with different polarities (water,
methanol and hexane) resulting in 159 extracts that were analyzed for radical scavenging
capacity, reducing power and metal chelating activity. Preliminary results have identified at least
9 promising species that will be further evaluated in terms of biological activity. The lab work
was in part assured by 4 BII students (Ana Rita Barradas, Janine Diogo, Manuel Vieira and Tiago
Justo) and by 2 undergraduate Biochemistry students (Carlos Gonçalves e Paulo Ferreira).
246
8d. Productivity
1. Ramos, A, Marques, A, Rodrigues, M, Henriques, N, Baumgartner, A, Castilho, R, Brenig, B,
Varela, J. 2009. Molecular and functional characterization of a cDNA encoding 4-hydroxy-3methylbut-2-enyl diphosphate reductase from Dunaliella salina. Journal of Plant Physiology 166:
968-977. IF=2.500.
2. Cravo, A, Lopes, B, Serafim, A, Company, R, Barreira, L, Gomes, T, Bebianno, MJ. 2009. A
multibiomarker approach in Mytilus galloprovincialis to assess environmental quality. Journal of
Environmental Monitoring 11: 1673-1686. IF= 2.225.
3. Bebianno, MJ, Barreira, LA. 2009. Polycyclic aromatic hydrocarbons concentrations and
biomarker responses in the clam Ruditapes decussatus transplanted in the Ria Formosa lagoon.
Ecotoxicology and Environmental Safety 72: 1849–1860. IF=2.133.
4. Custódio, L, Fernandes, E, Escapa, A. L, Aligué, R, Alberício, F, Romano, A. 2009. Antioxidant
activity and in vitro inhibition of tumor cell growth by leaf extracts from the carob tree
(Ceratonia siliqua L.). Pharmaceutical Biology 47: 721-728. IF=0.672.
proceedings)
proceedings)
PhD THESES
Ramos, A. 2009. Molecular basis of isoprenoid biosynthesis in Dunaliella salina. PhD thesis,
University of Algarve. Supervisor: J Varela, Co-Supervisor: Bertram Brenig (Göttingen, Germany).
AtlantikFish, S.A - Portuguese aquaculture company. Agreement for the development of novel
algal strains for biotechnological production of biodiesel from microalgal sources.
247
Necton S.A. - Portuguese biotechnology company. Agreement for the development of novel
algal strains for biotechnological production of biodiesel from microalgal sources. This
agreement was a consequence of the BOTRYOFUEL project.
Necton S.A. - Portuguese biotechnology company. Agreement for the determination of bioactive
compounds in microalgal pastes. This agreement was signed under the SEABIOMED project.
Our team collaborates with several research groups, which is displayed in joint publications in
our peer-reviewed publication track record. The groups we collaborate with include:
-
Parc Scientic Barcelona, Barcelona, Spain. Group Leader and Director: Dr. Fernando
Albericio. This collaboration was initiated in 2009 and it will be essential for the
bioassay-guided characterization of extracts (and fractions thereof) from micro-,
macroalgae and sea-grasses for the development of marine biopharmaceuticals.
-
Massachusetts Institute of Technology (MIT), USA. Group Leader: Prof. Achmed
Ghoniem. This collaboration is essential to develop novel ways of enhancing gas (CO2
and O2) exchange and delivery within photobioreactors for biofuel production.
-
University of Freiburg, Germany. Group Leader: Prof. Wolfgang Hess. This collaboration
will be essential for the systems biology approach to understanding how ecologically
relevant cyanobacteria regulate iron homeostasis in fresh water and marine
environements.
-
PharmaMar, Barcelona, Spain. Research & Development Director: Carmen Cuevas. A
wholly owned member of the Zeltia Group is a biopharmaceutical company determined
to advance cancer treatment through discovering, developing, producing, and
marketing innovative drugs of marine origin.
-
Laboratory of Molecular and Cellular Biology of Cancer (LBMC), Luxembourg. Group
Leader: Marc Diederich. This collaboration is of paramount importance for studies on
the antitumoral and anti-inflammatory activities.
-
Canary Technological Institute, Canary Islands, Spain. Group Leader: Dr. Héctor
Mendoza. This collaboration in resulted in a joint publication about the development of
novel techniques for high-throughput isolation of microalgal lipid hyperproducers.
-
Brooklyn College, NY, USA. Group Leader: Dr. Juergen Polle. This collaboration is part of
the international effort in sequencing the genome of Dunaliella salina with the help of
the US Department of Energy.
-
University of Nevada, USA. Group Leader: Dr. John Cushman. This collaboration is part
of the international effort in sequencing the genome of Dunaliella salina with the help
of the US Department of Energy.
248
8e. Future research
1. Objectives
As mentioned in the item Objectives, the MarBiotech research group has shifted its main
research interests from the elucidation of the molecular basis of the massive carotenoid
accumulation in the microalga D. salina to three novel research lines: 1) microalgae-derived
biofuels; 2) marine bioactive compounds; and 3) iron homeostasis in cyanobacteria. The first line
of research was the result of the research group contacts with the local biotechnology industry
present in Algarve, namely Necton S.A. (Belamandil, Olhão) and AtlantikFish S.A. (Castro Marim).
Our entrepreneurial partners wished to develop microalgal novel strains for massive production
of biofuel. The main strategy is to use autochthonous strains rather than those present in
culture collections, as the former are already adapted to the local conditions of abiotic factors
such as light intensity, salinity, nutrients and temperature as well as biotic factors such as
predation and competition. We established contacts with other research groups, namely those
led by Prof. Xavier Malcata (CIIMAR) and Prof. A. Ghoniem (MIT, USA), who propose to use
advanced flat panel technologies combined with novel ways of illuminating (e.g use of modified
light fibers) and expediting gas (CO2 and O2) exchange and delivery (e.g. use of modified tubing)
to microalgal cultures in order to optimize biomass growth and lipid / hydrocarbon induction for
optimal biofuel production. In alternative, we will explore new ways to enhance biomass and
lipid / hydrocarbon production by establishing biphasic protocols, in which biomass is
maximized during cellular growth and proliferation and lipid accumulation is enhanced at a later
stage upon exposure of cells to optimized lipidogenic (abiotic) conditions, when ideal cellular
concentrations are reached. Since the biodiesel production costs may be further reduced with
the optimization of biomass use, the biomass residue resulting from lipid extraction will be
further extracted in order to determine biological activities of the remaining compounds in a
biorefinery concept. These will include antioxidant, anti-tumoural, anti-diabetic, antiinflammatory, neuroprotective, antibiotic, insecticidal, leischmanicidal and allelopathic
activities. The determination of these activities is also the objective of the SEABIOMED project
which will begin in January 2010. This project involves the analysis of different marine
photosynthetic organisms such as micro and macroalgae, seagrasses and salt marsh plants. As
mentioned the item Objectives, several extracts from the samples gathered in the 2009
campaigns have already been analyzed for antioxidant capacity with some promising results
which will be further pursued by two scientific laboratory technicians to be contracted by the
SEABIOMED project in 2010 and 3 graduated students (MSc students from the MSc on Biological
Engineering and Medicine) which will also join the MarBiotech group in 2010. Whenever
relevant, the nutritional value of the plants will also be evaluated.
Concerning the third line of research, we will collaborate with Dr. M. Futschik’s and W. Hess’s
labs in order to elucidate how uptake and internal concentrations of iron are regulated at the
molecular level in a model cyanobacterium (Synechocystis spp.) and an ecologically relevant
cyanobacterium such as Prochlorococcus spp. This collaboration will be funded by the CYANO
249
research project, in which we will focus on protein and micro-RNA regulatory molecules that
have been identified previously as potential key players. We will extend their observations by
cloning additional players via the construction of subtraction cDNA libraries. Differentially
expressed clones will be studied further by means of microarray-derived expression profiles,
QtPCR and two-hybrid screens. We will build a network model of how regulatory/storage
players interact with each other functionally and/or physically in Synechocystis and
Prochlorococcus spp. Our results will contribute to a better understanding how aquatic
photosynthetic organisms regulate iron homeostasis, an important factor for global primary
productivity.
Lastly, we will continue our collaboration with Prof. J. Polle’s and J. Cushman’s labs concerning
the elucidation of Dunaliella salina genome. This development will be of extreme importance, so
that further insights can be gathered concerning how this extremophile adapts to high salinity,
high light and low levels of nutrients and enhances carotenoid biosynthesis.

Current funding
159.002 EUR, FCT PTDC/MAR/103957/2008. SEABIOMED - Marine Photosynthetic organisms of
the Algarve Coast with Biomedical Applications, 2010.
80.000 EUR, FCT PTDC/BIA-MIC/101036/2008, CYANO - Iron Homeostasis regulation in
cyanobacteria, 2010.

Pending funding
87.984 EUR, FCT PTDC/MAR/110715/2009. PROMETHEUS: PROduction, via Microalgae after
Engineering and Technological optimization, of Hydrocarbons and triglycerides for Engine Uses.
108.740 EUR, EU FP7-KBBE-2010-3.2-03 EMMERITUS: Engineering with, and Modification of
Microalgae for Enhanced Recovery and synthesIs in Technologically-feasible indUstrial Settings.
522.183 EUR, EU FP7-KBBE-2010-3.2-03 BioAlgae: The extraction of oil from algae for biodiesel
production.
250
Rui Orlando Pimenta Santos
-
31.948,62 EUR FCT Pluriannual funding.
-
77.928,66 EUR, FCT National funding.
FCT POCI/MAR/58427/2004.
FCT POCTI/MAR/55377/2004.
FCT POCTI/MAR/58172/2004.
FCT POCI/MAR/56956/2004.
FCT POCI/BSE/48918/2002.
-
5.195,54 EUR, Innovation Agency (AdI), Filtralgae
-
33.180 EUR, European Commission Funding
FP6-2004-SME-COOP.
SOE1/P2/F153.
-
61.448,86 EUR, Other funding
INAG, EEMA - Ecological status of coastal and transitional waters of the Portuguese coast.
Hydromod, Sines power plant Project.
251
1. Objectives
I. Metabolism of coastal systems:
This research line aimed to understand the role of coastal systems as sinks or sources of CO2. In
particular, we aimed to reveal the importance of biological communities dominated by
macrophytes for net ecosystem production. The underlying processes that determine if a
biological community is a sink or source of carbon were investigated, in particular the
environmental drivers of photosynthesis and community production, respiration and
calcification.
The specific objectives of this research line were:
I.1. To assess the carbon and nitrogen budgets of a coastal lagoon dominated by intertidal
seagrass and salt marsh meadows (Ria Formosa lagoon), including the organic matter and
nutrient imports from the watershed and their exchanges with the ocean. Furthermore, we
aimed to assess the metabolic processes that determine the carbon budget, how they respond
to the seasonal cycle and how they relate to the CO2 fluxes with the atmosphere.
I.2. To evaluate the combined effects of light availability and spectral composition on the
performance of the photosynthetic apparatus and the biochemical traits of deep-edge seagrass
plants to determine their responses to transient changes in PAR spectral composition. Turbidity,
and consequent alterations in light quantity and quality are crucial in determining the coastal
areas potentially occupied by seagrasses.
I.3. To investigate the relationships between photosynthesis and calcification in red calcareous
algae (mäerl), comparing southern and northern Iberian systems. Specifically, we aimed to
determine in situ the diel and annual patterns of calcification, photosynthesis and community
metabolism in natural mäerl populations and investigate the effects of increased CO2 on the
calcification and photosynthesis of the predominant species in Iberian waters.
II. Human impacts on coastal ecosystems:
This research line focused on the effects of human-related disturbances on costal ecosystems,
including physical disturbances, introduction of non-native species or global change effects such
as increased temperature and CO2/acidification. Our aim is to contribute with sound scientific
inputs to the conservation and management of these systems.
The specific objectives of this research line were:
II.1. To construct a mesocosm system with CO2 enrichment to develop experimental research
on the effects of increased CO2/acidification on the photosynthesis and production of seagrass
communities.
II. 2. To provide a detailed taxonomic description of the benthic macroalgae communities along
the Portuguese continental coast, so that comparisons can be made with previous descriptions
252
made in the 1950/60´s, in order to assess the long-term, temperature related, changes in the
flora.
II.3. To assess the population ecology traits of one of the most invasive marine algae, Sargassum
muticum, including its demographic traits and population dynamics and the potential role of
herbivores on regulating the invasion of Portuguese coastal waters.
II.4. To implement research on the human-induced shifts from seagrasses to seaweed
domination systems. Seagrass decline has been attributed to the eutrophication-related
development of bloom-forming seaweeds. We aim to assess, experimentally, the impact
patterns, critical thresholds and processes ranging from species, communities to ecosystem
levels in similar seagrass systems over a north-south gradient of hundreds of kilometers within
each of the three regions (Portugal, USA, New Zealand) at similar latitudes, covering 4 different
seagrass species in order to come to general applicable theories, models and management
tools.
II.5. Within the Water Framework Directive (WFD), we aimed to use macroalgae and marine
angiosperms as biological elements to classify the ecological status of the Portuguese coastal
and transitional water bodies, respectively. A specific objective was to develop new ecological
indicators of the condition of those communities, based on the detailed description of seaweed
richness and on the ecophysiological parameters of seagrasses.
II.6. To implement a European-wide research coordination network that integrates expertise in
physiological ecology, ecological genomics and conservation/resource management to provide
the scientific basis for preserving the goods and services arising from the productivity of
European seagrass ecosystems under anthropogenic pressure and to develop comprehensive
best practices for integrated seagrass habitat management.
In 2009 we published 19 papers and presented 12 communications at international scientific
meetings and 2 at national meetings. One Master thesis was concluded.
Our scientific contributions focused on the knowledge of the methods and applications to
measure seagrass photosynthesis and carbon uptake, on the environmental drivers of
photosynthesis of tropical macroalge and seagrasses, on the identification of Ulva blooms in
China and on the effects of nutrient enrichment on Ulva from the Red Sea, on the reproductive
biology of Zostera marina, on the morphological adaptations to local gradients of the seagrass
Zostera noltii, on the assessment of the impacts of sediment dynamics on Z. noltii meadows, on
the effects of herbivory on Sargassum muticum invasiveness, on the demographic traits that
determine S. muticum invasiveness and on the reproductive biology and ecology of S. muticum.
This past year we also made an extended contribution (6 papers) to the taxonomic description
of the benthic macroalgae communities along the Portuguese continental coast.
Two national projects that were concluded, “Carbon uptake by Ria Formosa intertidal
communities” (POCTI/MAR/58172/2004) and “Green macroalgal blooms in Ria Formosa and
adjacent coastal beaches” (POCI/MAR/58427/2004), provided a series of data on the seasonal
variation of carbon and nitrogen fluxes in Ria Formosa lagoon and on the role of seagrass,
saltmarsh and green algae communities on carbon and nitrogen uptake. Manuscripts are being
253
prepared on these subjects. An important achievement was the financing approval of the FCT
project “Whole-system metabolism and CO2 fluxes in a coastal lagoon dominated by saltmarsh
and seagrass meadows, PTDC/AAC-CLI/103348/2008. This project will further develop this
research line to attain the goal of quantifying the whole ecosystem metabolism of Ria Formosa
lagoon. The impacts of the human activity on the ecosystem metabolism will be assessed
simulating different usage scenarios in an ecological/hydrodynamic model of the system.
We also concluded a project financed by “Ministerio de Educación y Ciencia”, Spain (CGL200603576/BOS) on the conservation status of the mäerl communities of the Atlantic Iberian coast,
where we assessed the seasonal variation of photosynthetic production of mäerl communities in
southern Portugal and the metabolism and calcification rates of mäerl communities in Ria de
Arosa, Spain. To follow up this research line, we submitted a project to FCT to assess the
calcification, photosynthesis and metabolism of calcifying algae in an acidified ocean.
We obtained financing for more three projects:
-
Eco-Lagunes: Environmental management of coastal zones with aquaculture potential.
(SOE1/P2/F153), SUDOE (INTERREG IV B SUDOE), where we investigate suitable
ecological indicators for monitoring the health condition of seagrass communities based
on population and biochemical descriptors.
-
EEMA: Ecological status of coastal and transitional waters of the Portuguese coast,
under the Water Framework Directive, financed by INAG (Water Institute), where we
will use marine angiosperms, saltmarsh and macroalgae as biological elements to
classify the ecological status of the Portuguese coastal and transitional water bodies.
-
Shifts
from
seagrass
to
seaweed
dominated
systems
(INVASEA),
PTDC/MAR/098069/2008, to assess, the impact patterns, critical thresholds and
processes ranging from species, communities to ecosystem levels in similar seagrass
systems over a north-south gradient of hundreds of kilometers within each of the three
regions (Portugal, USA, New Zealand), in order to come to general applicable theories,
models and management tools.
We also obtained the financing approval from European Science Foundation to develop a COST
Action network that is chaired by the coordinator of the research group entitled “Seagrass
productivity: from genes to ecosystem management”. In close relationship with some of the
objectives of this network, we submitted a project to FCT with the aim of understanding how
light quality and quantity determines the photosynthetic performance and survival of
seagrasses.
Under the Association of European Marine Biological Laboratories financed by the European
Community (ASSEMBLE - EC/FP7/227799) we constructed a seagrass mesocosm system in the
Ramalhete Field Station of CCMAR, to maintain the 3 species of seagrasses present at Ria
Formosa lagoon, Zostera noltii, Z. marina and Cymodocea nodosa, and to test experimentally
the effects of CO2 enrichment on photosynthesis, production and respiration of seagrass
communities.
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8d. Productivity
1. Cunha, A, Santos, R. 2009. The use of fractal geometry to determine the impact of inlet
migration on the dynamics of a seagrass landscape. Estuarine, Coastal and Shelf Science 84: 584590. IF=1.970.
2. Monteiro, C, Engelen, AH, Santos, R. 2009. Macro- and mesoherbivores prefer native
seaweeds over the invasive brown seaweed Sargassum muticum: a potential regulating role on
invasions. Marine Biology 156: 2505-2515. IF=1.999.
3. Engelen, A, Santos, R. 2009. Which demographic traits determine population growth in the
invasive brown seaweed Sargassum muticum? Journal of Ecology 87: 675-684. IF=4.690.
4. Runcie, JW, Paulo, D, Santos, R Sharon, Y, Beer, S, Silva, J. 2009. Photosynthetic responses of
Halophila stipulacea to a light gradient: I - In situ Energy Partitioning of Non-photochemical
Quenching. Aquatic Biology 7: 143-152. IF=1.380.
5. Sharon, Y, Silva, J, Santos, R, Runcie, J, Chernihovsky, M, Beer, S. 2009. Photosynthetic
responses of Halophila stipulacea to a light gradient: II – Plastic Acclimations Following
Transplantations. Aquatic Biology 7: 153-157. IF=1.380.
6. Silva, J, Sharon, Y, Santos, R, Beer, S. 2009. Measuring seagrass photosynthesis: Methods and
applications. Aquatic Biology 7: 127-141. IF=1.380.
7. Berecibar, E, Wynne, MJ, Bárbara, I, Santos, R. 2009. New records of Rhodophyta to the flora
of the coast of the Iberian Peninsula. Botanica Marina 52:217-228. IF=1.090.
8. Cabaço, S, Machás, R, Santos, R. 2009. Individual and population plasticity of the seagrass
Zostera noltii along a vertical intertidal gradient. Estuarine, Coastal and Shelf Science 82: 301308. IF=1.970.
9. Berecibar, E, Wynne, MJ, Santos, R. 2009. Contarinia squamariae (Rhizophyllidaceae,
Rhodophyta): first report from Portugal. Botanica Marina 52: 15-23. IF=1.090.
10. Díaz, P, Berecibar, E, Bárbara, I, Cremades, J, Santos, R. 2009. Biology and taxonomic identity
of Erythroglossum lusitanicum (Ceramiales, Rhodophyta) from the Iberian Peninsula. Botanica
Marina 52: 207-216. IF=1.090.
11. Araújo, R, Bárbara, I, Tibaldo, M, Berecibar, E, Díaz Tapia, P, Pereira, R, Santos, R, Sousa
Pinto, I. 2009. Checklist of benthic marine algae of Northern Portugal. Botanica Marina 52: 2446. IF=1.090.
12. Monteiro, C, Engelen, AH, Serrão, EA, Santos, R. 2009. Habitat differences in the timing and
reproduction of the invasive alga Sargassum muticum (Phaeophyta, Sragassaceae) over tidal and
255
13. Berecibar, E, Wynne, MJ, Cremades, J, Santos, R. 2009. Report of the brown alga
Zosterocarpus oedogonium (Ectocarpales) from Portugal, its first recorded occurrence outside of
the Mediterranean Sea. Nova Hedwigia 89: 237-244. IF=0.763.
14. Berecibar, E, Wynne, MJ, Santos, R. 2009. Report of the red alga Gulsonia nodulosa
(Ceramiales) from Portugal, its first recorded occurrence outside of the Mediterranean Sea.
Nova Hedvigia 88: 23-31. IF=0.763.
15. Figueroa, FL, Martínez, B, Israel, A, Neori, N, Malta, E-j, Ang, Jr P, Inken, S, Marquardt, R,
Rachamim, T, Arazi, U, Frenk, S, Korbee, N. 2009. Acclimation of Red Sea macroalgae to solar
radiation: photosynthesis and thallus absorptance. Aquatic Biology 7: 159-172. IF=1.380.
Korbee, N. 2009. Effects of nutrient supply on photosynthesis and pigmentation in Ulva lactuca
(Chlorophyta): responses to short-term stress. Aquatic Biology 7: 172-183. IF=1.380.
17. Leliaert, F, Zhang, XW, Ye, NH, Malta, E, Engelen, AH, Mineur, F, Verbruggen, H, De Clerck, O.
2009. Research note: Identity of the Qingdao algal bloom. Phycological Research 57: 147-151.
IF=1.246.
In press
18. Cabaço, S, Santos, R. (in press). Reproduction of the eelgrass Zostera marina at the species
southern distributional limit in the eastern Atlantic. Marine Ecology, Doi: 10.1111/j.14390485.2009.00331.x. Published on-line 25 Sept.
19. Silva, J, Santos, R. 2009. Reply to comments on “Underwater measurements of carbon
dioxide evolution in marine plant communities: a new method” by Gwenaël Abril. Estuarine,
Coastal and Shelf Science doi:10.1016/j.ecss.2008.11.012. IF=1.970.
proceedings)
1. Engelen, AH, Santos, RO. 2009. Invasion ecology of Sargassum muticum, the invader par
excellence. Proceedings of the 9th International Phycological Congress, Tokyo, Japan. Phycologia
48(4): 29.
proceedings)
MASTER THESES
Vicêncio, SC. 2009. Distribuição de macroalgas nativas e exóticas no substrato duro subtidal do
Porto de Sines. Master thesis in Ecology, Management and Modelling of Marine Resources, New
University of Lisbon. Supervisor: R Santos. (Submitted in 2009, defense in Jan 2010)
256
Organization of a workshop in Faro, Portugal, to implement two seagrass monitoring sites in Ria
Formosa lagoon, included in the world-wide SeagrassNet monitoring program
(www.seagrassnet.org).
12 of the 19 papers published (63%) were done in collaboration with international research
teams. The group presented communications in 7 international scientific meetings. The group is
currently involved in 3 international research projects.
The research group is involved in the COST Action ES0906, “Seagrass productivity: from genes to
ecosystem management”, which is chaired by the group leader. The network aims to improve
the scientific collaboration among European research groups and to bridge the gap between
scientists and seagrass managers. It involves 14 European countries and researchers from
Australia and USA.
We contributed to the preparation of the final Report of the European Community project
Envirophyte - Improvement of the cost effectiveness of marine land-based aquaculture facilities
through use of Constructed Wetlands with Salicornia as an environmentally friendly biofilter and
a valuable by-product, FP6-2004-SME-COOP.
We have an active role on the Association of European Marine Biological Laboratories financed
by the European Community (ASSEMBLE - EC/FP7/227799), particularly on the development and
maintenance of seagrass mesocosms at CCMAR, and interacting with visiting scientists that
apply to use this facility.
A team member integrates the World Seagrass Association Steering Committee, as one of the
two European representatives in this organ.
Team members integrate Editorial Boards of indexed international scientific journals such as
Botanica Marina, Marine Ecology and Marine Environmental Research. The group leader is an
Associate Editor of Estuaries and Coasts.
The group leader is coordinating the seagrass sub-group for the North East Atlantic Geographical
Intercalibration Group (NEAGIG) in the ambit of the Water Framework Directive.
257
The group leader was invited to chair mini-symposium sessions on the Coastal and Estuarine
Research Federation Congress in Portland, USA and on the XX International Seaweed
Symposium, Ensenada, Mexico.
The research group is responsible for the seasonal monitoring of two sites in Ria Formosa
lagoon, for the world-wide SeagrassNet monitoring program (www.seagrassnet.org).
A first year report was prepared for INAG (Water Institute of Portugal) in the ambit of the
project EEMA: Ecological status of coastal and transitional waters of the Portuguese coast,
under the Water Framework Directive.
8e. Future research
1. Objectives
Research will continue to be developed to attain the general aims of the two main research
areas of the group, I. Metabolism of coastal systems and II. Human impacts on coastal
ecosystems, presented above in section Objectives and Achievements.
More specifically for the newly approved projects, the research objectives of our group for the
next years are:
Whole-system metabolism and CO2 fluxes in a coastal lagoon dominated by saltmarsh and
seagrass meadows (PTDC/AAC-CLI/103348/2008).
-
To use a multi methodological approach to assess whole-system metabolism and CO2
fluxes of Ria Formosa lagoon, including estimations of the connectivity of the system
with watershed and the ocean. Given the high uncertainties associated with the
estimates of net ecosystem production (NEP) we will use a three method approach that
includes incubation methods, the direct measurement of air-water CO2 fluxes and the
stoichiometric approach proposed by the Land Ocean Interaction in the Coastal Zone,
LOICZ, of the International Geosphere-Biosphere Program (IGBP).
Seagrass productivity: from genes to ecosystem management (COST Action ES0906).
-
258
To form a European-wide research coordination network that integrates expertise in
physiological ecology, ecological genomics and conservation- resource management.
The European capacities for seagrass research will be integrated to carry out six tasks: 1)
establish continuous, in situ, measurement devices for seagrass productivity, 2)
establish a modelling and monitoring tool based on seagrass light requirements, 3)
understand seagrass responses to global changes, 4) assess seagrass genetic adaptative
variation at photosynthetic related loci, 5) evaluate the effectiveness of existent and
new seagrass-health ecological indicators and 6) provide guidelines to improve the
conservation and management of seagrass dominated ecosystems.
Under the Association of European Marine Biological Laboratories financed by the European
Community (ASSEMBLE - EC/FP7/227799) we aim to develop experiments in collaboration with
international visiting scientists to test the effects of increasing CO2/acidification and nutrients
on photosynthesis, production, respiration and calcification of seagrass and algal communities
using both mesocosms and laboratory microcosms.
EMA: Ecological status of coastal and transitional waters of the Portuguese coast, under the
Water Framework Directive.
-
Within the Water Framework Directive (WFD), we aim to use marine angiosperms
(seagrasses and saltmarshes) and macroalgae as biological elements to classify the
ecological status of the Portuguese coastal and transitional water bodies. A specific
objective is to develop new ecological indicators of the condition of those communities,
based on detailed description of seaweed richness (Coastal Waters) and on
ecophysiological parameters of seagrasses (Transitional Waters).
Gestion environnementale des zones lagunaires à vocation aquacole (ECO-LAGUNES).
-
To assess select adequate biological indicators for monitoring the ecological condition of
seagrass communities. Indicators will be of two categories, a) population indicators used
in the Water Framework Directive such as species area cover, biomass and density, b)
biochemical indicators such as pigment content, soluble protein and sugars, C and N
content, N stable isotope ratios, oxidative stress indicators such as malondialdehyde
(MDA).
Shifts from seagrass to seaweed dominated systems (INVASEA).
-
The main objective of this project is to assess consequences and their mechanisms of
seaweeds invasions into seagrass dominated systems over various geographical scales
using seaweeds species with different taxonomical background and life history
strategies in order to develop less biased theories and implement the acquired
knowledge into management strategies.
Other potential objectives to attain in the next years are those related to submitted projects
pending for funding:
Seagrass photosynthesis in changing light
(PTDC/MAR/113379/2009).
-
environments:
understanding the
limits
This project aims to answer a question that is relevant to both seagrass biology and
ecosystem conservation: How will the vertical distribution of seagrasses be affected by
significant reductions in the amount of available photosynthetic active radiation (PAR)
and profound alterations of its spectral composition, resulting from increased
eutrophication and siltation phenomena in the coastal waters?
259
Mäerl calcification, photosynthesis and metabolism in an acidified ocean (PTDC/BIABEC/101198/2008).
-
This project has two main objectives, (i) to determine the annual patterns of
calcification, photosynthesis and community metabolism in natural mäerl populations of
Phymatholithon calcareum and Lithothamnion corallioides, the two species found in the
Iberian Peninsula and (ii) to predict how increased CO2 and ocean acidification on the
calcification and photosynthesis of Phymatholithon calcareum and Lithothamnion
corallioides, the predominant mäerl species in Iberian waters.
Waterborne seaweed metabolites as a green solution for antimicrobial therapy in aquaculture
(PTDC/MAR/098463/2008).
-
This proposal aims to provide an alternative means for antimicrobial control to
antibiotics using waterborne seaweed metabolites. It will identify the benefits of
integrating seaweeds in the animal aquaculture industry as a novel means to reduce
bacterial infections in intensive fish production. The specific objectives will be to:
-
Quantify the production/release rates of Asparagopsis taxiformis metabolites to
the surrounding water and determine their residence times.
-
Characterize the planktonic bacterial community composition in A. taxiformis
conditioned seawater using molecular microbial techniques.
-
Determine the benefits of waterborne A. taxiformis metabolites for seabream
larvae culture.
-
Determine the activity of waterborne A. taxiformis metabolites against the fish
pathogenic bacteria Pseudomonas anguilliseptica and Listonella anguillarum.
-
Evaluate the potential benefits of waterborne seaweed metabolites for
seabream aquaculture with challenge experiments.
DiverseShores - Testing associations between genetic and community diversity in European
rocky shore environments (PTDC/BIA-BIC/114526/2009).
- We aim to test hypotheses consistent with the suggestion that similar processes result
in comparable spatial distributions of community and genetic diversity at two spatial
scales, firstly, along the Portuguese coast and secondly, the North Atlantic. We aim to
test whether: 1) local (alfa) community diversity is positively correlated with
intraspecific (within species) genetic diversity of the constituent species; 2) between
area (beta) community diversity is positively correlated with gene flow between
conspecific populations of constituent species; and 3) both genetic diversity and
community diversity depend upon environmental variables.
260

Current funding
100.828 EUR, Interreg Sudoe, SOE1/P2/F153, Gestion environnementale des zones lagunaires à
vocation aquacole (ECO-LAGUNES), European Community, 2009-2011.
229.319 EUR, INAG, EEMA - Ecological status of coastal and transitional waters of the
Portuguese coast, under the Water Framework Directive, 2009-2011.
199.256 EUR, FCT PTDC/AAC-CLI/103348/2008, Whole-system metabolism and CO2 fluxes in a
coastal lagoon dominated by saltmarsh and seagrass meadows, 2010-2012.
170.362 EUR, FCT PTDC/MAR/098069/2008, INVASEA - Shifts from seagrass to seaweed
dominated systems, 2010-2012.
65.000 EUR (2010), COST Action ES0906, Seagrass productivity: from genes to ecosystem
management, 2010-2014.

199.944 EUR, FCT PTDC/BIA-BEC/101166/2008, Seagrass photosynthesis in changing light
environments: understanding the limits.
199.267 EUR, FCT PTDC/BIA-BEC/101198/2008, Mäerl calcification, photosynthesis and
metabolism in an acidified ocean.
185.594 EUR, FCT PTDC/MAR/098463/2008, Waterborne seaweed metabolites as a green
solution for.antimicrobial therapy in aquaculture.
55.534 EUR (CCMAR), FCT PTDC/BIA-BIC/114526/2009, DiverseShores - Teste da associação
entre diversidade genética e diversidade da comunidade em costas rochosas Europeias.
261
Rodrigo da Silva Costa
-
-
National funding
179.732 EUR, FCT PTDC/MAR101431/2008, Unlocking the bacterial diversity encrypted in the
metagenomes of marine sponges, 2009-2011. PI: R Costa.
600 EUR, FCT PTDC/AAC-CLI/107916/2008, Global climate change and pollution: a synergy
designed for disaster? Team member: R Costa.
1. Objectives
The research programme in the Laboratory of Microbial Ecology and Evolution focuses on the
interactions that microbes establish with their (macro) eukaryotic hosts. Upon such interactions,
microbes act as engineers of new, distinct and dynamic microenvironments on their own that
arise, to a large extent, as a consequence of their intense metabolic activity. Under these
circumstances, the expression and biosynthesis of genes and metabolites involved in biological
warfare (competition, antagonism) and protocooperation (mutualism, symbiosis) are favoured.
Currently, microbial symbionts of marine sponges, bryozoans and seaweeds constitute the
targets under investigation within our framework.
Microbes comprise a wide, polyphyletic group of organisms that, together, encompass by far the
largest resource of metabolic and genetic diversity encountered on Earth. The major part (c. 9599%) of the microbiota occuring in open ecosystems is recalcitrant to cultivation in the
laboratory. This hinders our ability to readily access this truly astounding genetic and metabolic
reservoir. The Laboratory of Microbial Ecology and Evolution applies new, cutting-edge
262
technologies that circumvent less resolving approaches in unlocking the genetic resources
encrypted in natural microbial communities.
The group aims at:
-
Unravelling and exploiting the extant genetic diversity, in functional and phylogenetic
terms, of microorganisms in association with Eukarya and the potential biotechnological
applications thereof;
-
Adrressing the putative roles of microbial symbionts in host house-keeping functions
and secondary metabolite production;
-
Establishing gene function databases for microbial symbionts of marine organisms;
-
Monitoring the variation in structure and composition of microbial guilds (e.g., Bacteria,
Fungi, Archaea) inhabiting (macro) eukaryotic hosts across spatial and temporal scales,
enabling data interpretation under an ecological context.
-
To achieve these goals, the group will center its approach on high throughput
sequencing of microbial DNA/RNA extracts directly retrieved from host tissues (i.e.,
metagenomics and metatranscriptomics, respectively) along with innovative strategies
for extensive cultivation of microorganisms retrieved from marine Eukarya.
-
Two publications in top journals in the area of Microbial Ecology (1 report in
Environmental Microbiology, IF 2007=4.929; 1 report in Applied and Environmental
Microbiology, IF 2007=4.004).
-
Acquisition of research funds (budget: 179.732,00 EUR) through the project entitled
“Exploring the bacterial diversity encrypted in the metagenomes of marine sponges”
(PTDC/MAR/10431/2008), in which Rodrigo Costa acts as principal investigator.
-
Participation, as team member, in one (1) Portuguese funded project entitled “Global
climate change and pollution: a synergy designed for disaster?” (PTDC/AACCLI/107916/2008).
8d. Productivity
1. Hardoim, CCP, Costa, R, Araújo, FV, Hajdu, E, Peixoto, R, Lins, U, Rosado, AS, van Elsas, JD.
2009. Bacterial diversity in the marine sponge Aplysina fulva in Brazilian coastal waters. Applied
and Environmental Microbiology 75: 3331-3343. IF=3.686.
263
2. Costa, R, van Aarle, I, Mendes, R, van Elsas, JD. 2009. Genomics of pyrrolnitrin biosynthetic
loci: evidence for conservation and whole-operon mobility within Gram-negative bacteria.
Environmental Microbiology 11: 159-175. IF=4.909.
proceedings)
proceedings)
MASTER THESES
Keller, T. 2009. Bacterial diversity in and cytotoxic potential of Pseudomonas spp. associated
with the freshwater sponge Ephydatia fluviatilis. Master thesis, Friedrich-Schiller University of
Jena, Germany. Supervisor: Jan Dirk van Elsas (University of Groningen, The Netherlands),
Kirsten Küsel (Friedrich-Schiller University of Jena, Germany), Co-supervisor: R Costa.
All publications of the PI in the indicated period are the result of international collaborative
efforts.
A good example of international networking is the research on the microbial diversity in the
marine sponge Aplysina fulva, which has already resulted one publication in a renowned
international journal. The research project is still ongoing and now involves research institutions
in Brazil (Rio de Janeiro University), The Netherlands (University of Groningen) and Portugal
(CCMAR-CIMAR). Likewise, research on the bacterial communities found in association with the
freshwater sponge Ephydatia fluviatilis is in progress. It has already resulted in the elaboration
of a completed master thesis (M.Sc. Tina Keller, University of Jena) and will lead to the
publication of two scientific reports in international peer-reviewed journals. This work unites
264
research institutions based in three European countries (University of Groningen, The
Netherlands; Darmstadt University of Technology, Germany; and CCMAR-CIMAR, Portugal). In
this context, the PI was responsible for the scientific training of MSc. Tina Keller (University of
Jena, Germany) and MSc. Cristiane C.P. Hardoim (University of Groningen).
Current international partners of the Microbial Ecology and Evolution Laboratory are:
-
Prof. Dr. Jan Dirk van Elsas, Department of Microbial Ecology, University of Groningen,
Netherlands.
-
Prof. Dr. Alexandre Soares Rosado, Laboratório de Ecologia Microbiana Molecular
(LEMM), Instituto de Microbiologia Prof. Paulo de Góes (IMPPG), Universidade Federal
do Rio de Janeiro (UFRJ), Brazil.
-
Prof. Dr. Ulysses Lins, Laboratório de Ultraestrutura de Procariotos, Instituto de
Microbiologia Prof. Paulo de Góes (IMPPG), Universidade Federal do Rio de Janeiro
(UFRJ), Brazil.
-
Dr. Alexandre Jousset, Darmstadt University of Technology, Institute for Zoology,
Darmstadt, Germany.
-
Prof. Dr. Gabriele Berg, Graz University of Technology, Institute for Environmental
Biotechnology, Austria
The group is currently negotiating future collaboration with Prof. Dr. Kornelia Smalla (Julius
Kühn-Institut Bundesanstalt für Kulturpflanzen - JKI - Braunschweig, Germany).
8e. Future research
1. Objectives
See section Objectives and Main Achievements for general objectives of the working group.
More specific objectives of future research are as follows:
-
To determine the actual extent of microbial community diversity in benthic marine
invertebrates, using sponges and bryozoans as model target organisms;
-
To reveal the extent to which the shape of sponge- and bryozoa-associated microbial
communities is driven by the host animal and its biogeographical background;
-
To distinguish between facultative and obligate symbionts of marine invertebrates, and
develop genomics tools to specifically detect these symbionts in situ.
265
-
To identify bacteria isolated from benthic marine invertebrates and metabolites thereof
displaying cytotoxic activity;
-
To generate a database of bacterial polyketide synthase-encoding genes (PKS) retrieved
from benthic marine invertebrates. Bacterial polyketides are secondary metabolites
with high cytotoxic activity.

Current funding
3.000 EUR, expected FCT Pluriannual funding. 179.732 EUR, FCT PTDC/MAR101431/2008,
Unlocking the bacterial diversity encrypted in the metagenomes of marine sponges, 2009-2011.
PI: R Costa.

Pending funding
195.219 EUR, FCT PTDC/MAR/110020/2009, Find symbiont: a molecular-driven approach to
discover, localize and explore the bacterial partners of marine Bryozoa. PI: R Costa.
62.832 EUR, FCT PTDC/MAR/114347/2009, BICS - Bivalve filter feeding behaviours and their
impact on the community structure. Team member: R Costa.
266
Luísa Maria Pinheiro Valente
-
-
National funding
FCT PTDC/MAR/105229/2008, BenefitS - Benefícios da produção de macroalgas marinhas em
sistemas de aquacultura integrada multitrófica. PI: IS Pinto (CIIMAR - Biodiversity of Aquatic
Ecosystems Group).
45.000 EUR (CIIMAR), AdI, QREN, PROAMBIENTE, Dietas sustentáveis e de reduzido impacto
ambiental para o linguado senegalês. In consortium with the company A. Coelho e Castro, Lda.
Total funding: 599.700,74 EUR, 07/2009-06/2012. Scientific coordinator: L Valente.
188.327 EUR, FCT PTDC/MAR/70858/2006, TEXBREAM - Post-mortem changes in gilthead sea
bream muscle proteins: its implications to flesh texture, 2008-2011. Coordinator: J Dias (CCMAR
- Aquagroup).
40.200 EUR, QREN, ECOPISCIS - Waste Management in Fish Production, project 3442. Total
funding: 591.369 EUR, 2009-2011. Coordinators: JF Gonçalves (ICBAS), R Ozório.
-
European funding
49.970 EUR (CIIMAR), EU FP6-2005-SSP5A, SEACASE - Sustainable extensive and semi-intensive
coastal aquaculture in Sothern Europe. Total funding: 2.391.401 EUR; CIMAR-LA 312.486 EUR,
2007-2009. Partners: LANUCE, IPIMAR and involving researchers of 5 European Countries
(Portugal, Spain, France, Italy and Greece). Coordinator: MT Dinis (CCMAR - Aquagroup).
267
1. Objectives
The central aim of the group is to contribute to the sustainable development of the aquaculture
industry, through basic and applied research directed to the optimisation of diets and cultivation
techniques in order to improve growth and muscle quality. Special attention has been given to
the introduction of new species to aquaculture, namely the blackspot seabream (Pagellus
bogaraveo), the sole (Solea senegalensis), the bream (Diplodus sargus) and the octopus
(Octopus vulgaris).
Ongrowing projects deal with the following aspects:
-
Nutritional requirements and optimization of dietary regimes in important species for
aquaculture: Formulation and production of fish diets which promotes maximum
growth performance and better final fish quality; low pollution diets are also
considered. Dietary nutrient metabolism studies involve standard laboratory facilities
for proximate analysis of feed and fish tissues and activity and regulation of key
enzymes involved in lipid metabolism.
-
Growth characterization and flesh quality: Biochemical, histological and histochemical
parameters, namely the tissue fat composition, number and size of muscle fibres and
capacity of recruitment of new fibres are analysed under different rearing conditions.
The laboratory not only uses recent sensory, physical and microbiological analysis to
characterize the “freshness level” of several kinds of seafood, but also is responsible for
the creation of new sensory schemes for cephalopods under the recently developed
Quality Index Method. In the physical methods, this laboratory is using Torrymeter and
Freshmeter instrumental analysis. In the microbiological area, the use of counts of
specific spoilage organisms was developed in practical terms.
-
Olfaction is important in the appetite behavior of fish species, which eventually will
affect feeding and growth performance. Our group will begin in 2009 several research
trials in collaboration with CCMAR to determine olfactory potency of several extracts
and whether the homogenates can be added to commercial aqua feed pellets to
enhance their ingestion.
Nutrient requirements, dietary formulations and feeding practices were optimized for blackspot
seabream, Senegalese sole and bream. Blackspot seabream has a 50% dietary protein
requirement but low lipid tolerance (<10%). Optimal growth of sole is obtained with 8% lipids;
the estimated lysine requirement (4.68 g lysine / 16 g N) was used to design diets using plant
protein sources. Output: 7 papers (+ 1 submitted); 2 PhD, 1 MSc.
Protein requirement for Diplodus vulgaris was 7.8 g /kg body weight, and this species performed
better than D. sargus. Output: 1 paper.
268
The bottleneck of Octopus culture is the rearing of its paralarval. Microalgal species were used
to improve the biochemical composition of juvenile Artemia as prey for paralarvae. Output: 3
papers published, 1 PhD.
The production of ornamental aquatic species has now evolved into an industrial activity.
Information regarding nutritional requirements, food utilization and behaviour of ornamental
fish were considered. Output: 3 papers, 2 submitted.
Understanding the environmental factors that regulates muscle differentiation and growth is
important to select the best strategy to optimize growth and meat quality a) High-protein diets
favoured muscle hyperplasia; b) High temperature accelerates development and muscle growth
promoting hyperplastic growth; high dietary lipids reduces growth and expression of several
genes. Output: 6 papers, 1 submitted; 2 PhD.
Valorisation of aquaculture products. Production of functional foods. The use of dietary
conjugated linoleic acid (CLA) by fish has a potential benefit for human health. Being fish an
important source of protein and polyunsaturated fatty acids, a further increase in its CLA
content could enhance the nutritional value of fish meat for human consumption. When
supplemented, fish show the highest muscle CLA deposition of any other animal. CLA can be
incorporated up to 1 % in both rainbow trout and sea bass diets contributing to the production
of a functional food. Output: 5 papers, 2 MSc.
Sensory, physical and microbiological characterization of freshness in sea products: New sensory
schemes for cephalopods were developed under the Quality Index Method that was compared
and confirmed using other methods, namely physical methods (Torrymeter and Freshmeter) and
counts of specific spoilage organisms. Output: 7 papers.
8d. Productivity
1. Barata, EN, Hubert, F, Conceição, LEC, Velez, Z, Rema, P, Hubbard, PC, Canário, AVM. 2009.
293: 100-107. IF=1.925.
2. Barrento, S, Marques, A, Teixeira, B, Carvalho, ML, Vaz-Pires, P, Nunes, ML. 2009.
Accumulation of elements (S, As, Br, Sr, Cd, Hg, Pb) in two populations of Cancer pagurus:
Ecological implications to human consumption. Food and Chemical Toxicology 47: 150-156.
IF=2.114.
3. Borges, P, Oliveira, B, Casal, S, Dias, J, Conceição, L, Valente, LMP. 2009. Dietary lipid level
affects growth performance and nutrient utilization of Senegalese sole juveniles. British Journal
of Nutrition 102: 1007-1014. IF=3.446.
4. Dias, J, Conceição, L, Ribeiro, AR, Borges, P, Valente, LMP, Dinis, MT. 2009. Practical diet with
low fish-derived protein is able to sustain growth performance and reduce soluble phosphorous
loads in gilthead seabream (Sparus aurata). Aquaculture 293: 255-262. IF=1.925.
269
5. Figueiredo-Silva, AC, Corraze, G, Rema, P, Sanchez-Gurmaches, J, Gutiérrez, J, Valente, LMP.
2009. Blackspot seabream (Pagellus bogaraveo) lipogenic and glycolytic pathways appear to be
more related to dietary protein level than dietary starch type. Aquaculture 291: 101-110.
IF=1.925.
6. Gouveia, KG, Proença, PF, Roque da Costa, JC, Vaz-Pires, P, Martins da Costa, P. 2009.
Assessment of the efficiency of captive bolt stunning in cattle and feasibility of associated
behavioural signs. Animal Welfare 18: 171-175. IF=1.209.
7. Gouveia, KG, Vaz-Pires, P, Martins da Costa, P. 2009. Welfare assessment of broilers through
examination of haematomas, foot pad dermatitis, scratches and breast blisters at processing.
Animal Welfare 18: 43-48. IF=1.209.
8. Martins, DA, Valente, LMP, Lall S. 2009. Apparent digestibility of lipid and fatty acids in fish oil,
poultry fat and vegetable oil diets by Atlantic halibut, Hippoglossus hippoglossus L. Aquaculture
294: 132-137. IF=1.925.
9. Martins da Costa, P, Belo, A, Gonçalves, J, Bernardo, F. 2009. Field trial evaluating changes in
prevalence and patterns of antimicrobial resistance among Escherichia coli and Enterococcus
spp. isolated from growing broilers medicated with enrofloxacin, apramycin and amoxicillin.
Veterinary Microbiology 139: 284-292. IF=2.874.
10. Ozório, ROA. 2009. Dietary L-carnitine supplementation to cultivated fish: A mini-review.
Current Nutrition & Food Science 5: 40-48.
12. Ozório, ROA, Andrade, C, Conceição, LEC, Timóteo, VMFA, Valente, LMP. 2009. Effects of
feeding levels on growth response, body composition, and energy expenditure in blackspot
seabream, Pagellus bogaraveo, juveniles. Journal of the World Aquaculture Society 40: 95-103.
IF=0.780
13. Ozório, ROA, Valente, LMP, Correia, S, Pousão-Ferreira, P, Damasceno-Oliveira, A, Escório, C,
seabream (Diplodus vulgaris) juveniles. Aquaculture Nutrition 15: 85-93. IF=1.482.
14. Rema, P, Martins, J. 2009. Growth and survival of Carassius auratus larvae fed commercial
micro diets. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61(3): 714-720. IF=0.349.
15. Silva, JMG, Espe, M, Conceição, LEC, Dias, J, Valente, LMP. 2009. Senegalese sole juveniles
dietary amino acids are balanced. Aquaculture 296: 309-317. IF=1.925.
Motililty 30: 29-39. IF=1.657.
hatching to juvenile. Journal of Fish Biology 74: 37-53. IF=1.226.
270
18. Gouveia, KG, Ferreira, PG, Roque da Costa, JC, Vaz-Pires, P, Martins da Costa, P. 2009.
Assessment of the efficiency of captive-bolt stunning in cattle and feasibility of associated
behavioural signs. Animal Welfare 18, 171-175. IF=1.209.
19. Gouveia, K., Martins da Costa, P. and Vaz-Pires, P. 2009. Welfare assessment of broilers
through examination of haematomas, foot pad dermatitis, scratches and breast blisters at
processing. Animal Welfare 18(1): 43-48. IF=1.209.
20. Barrento, S, Marques, A, Teixeira, B, Anacleto, P, Vaz-Pires, P, Carvalho, ML, Nunes, ML.
2009. Macro and trace elements in two populations of brown crab Cancer pagurus: Ecological
and human health implications. Journal of Food Composition and Analysis 22(1): 65-71.
IF=2.423.
In press (published on-line in 2009)
21. Barrento, S et al. (in press). Live shipment of immersed crabs Cancer pagurus from England
to Portugal and recovery in stocking tanks: stress parameter characterization. ICES Journal of
Marine Science. Doi:10.1093/icesjms/fsp268. IF=1.920.
22. Cardoso, C et al. (in press) Effect of dietary fibre and MTGase on the quality of mackerel
surimi gels. Journal of the Science of Food and Agriculture. Doi: 10.1002/jsfa.363. IF=1.386.
23. Figueiredo-Silva, AC et al. (in press). Dietary Protein/Lipid level and protein source effects on
growth, tissue composition and lipid metabolism of Blackspot Seabream (Pagellus bogaraveo)
Aquaculture Nutrition. Doi: 10.1111/j.1365-2095.2009.00649.x. IF=1.482.
24. Martins da Costa, P et al. (in press). Changes in antimicrobial resistance among faecal
enterococci isolated from growing broilers prophylactically medicated with three commercial
antimicrobials. Preventive Veterinary Medicine. Doi: 10.1016/j.prevetmed.2009.09.012.
IF=2.121.
25. Ozório, ROA et al. (in press). Growth, nitrogen gain and indispensable amino acid retention
of pacu (Piaractus mesopotamicus, Holmberg 1887) fed different brewers yeast (Saccharomyces
cerevisiae) levels. Aquaculture Nutrition. Doi: 10.1111/j.1365-2095.2009.00662.x. IF=1.482.
26. Ozório, ROA et al. (in press). Effects of exercise on L-carnitine and lipid metabolism in African
catfish (Clarias gariepinus) fed different dietary L-carnitine and lipid levels. British Journal of
Nutrition. Doi: 10.1017/S0007114509993035. IF=3.446.
27. Seixas, P et al. (in press). High DHA content in Artemia is ineffective to improve Octopus
vulgaris paralarvae rearing. Aquaculture. Doi: 10.1016/j.aquaculture.2009.12.021. IF=1.925.
28. Silva, JMG et al. (in press). Aquaculture Research. Doi: 10.1111/j.1365-2109.2009.02451.x.
IF=1.099.
29. Valente, LMP et al. (in press). Animal Physiology and Animal Nutrition. Doi: 10.1111/j.14390396.2008.00900.x. IF=1.229.
271
30. Campos, C et al. (in press). Journal of Experimental Biology. Doi: 10.1242/jeb.033126.
IF=2.722.
proceedings)
1. Ozório, ROA. 2009. L-Carnitine and Energy Metabolism in Aquaculture. LAP Lambert Academic
Publishing AG & Co. KG, ISBN 978-3-8383-0560-8.
2. Moraes, JRE, Iwashita, MKP, Ozório, ROA, Rema, P, Moraes, FR. 2009. Influence of vitamin E
supplementation on dermal wound healing in Tilapia, Oreochromis niloticus. In: De Carlo, F,
Bassano, A (Eds), Freshwater Ecosystems and Aquaculture Research. Nova Science Publishers,
ISBN: 978-1-60741-707-1.
3. Silva, P, Valente, LMP, Malhão, F, Galante, MH, Monteiro, RAF, Rocha, E. 2009. Fibre types in
the skeletal muscle of blackspot seabream (Pagellus bagaraveo Brunnich) juveniles: a
histochemical immunohistochemical and ultrastructural study. Microscopy and Microanalysis
15(S3): 43-44. IF= 2.992.
proceedings)
1. Ozório, ROA, Valenti, WC. 2009. Current status and future perspectives of the Brazilian
aquaculture sector. In: Livro de Comunicações do XVIII Congresso de Zootecnia and II Congresso
Ibero-Americano de Zootecnia, 6-9 Maio 2009, Vila Real, Portugal, pp. 546-553, ISBN: 978-98996219-1-6.
2. Cruzeiro, C, Rema, P, Dias, J. 2009. O uso de anticorpos como aditivo na produção de truta
arco-íris (Onchorhynchus mykiss). In: Livro de Comunicações do XVIII Congresso de Zootecnia
and II Congresso Ibero-Americano de Zootecnia, 6-9 Maio 2009, Vila Real, Portugal, pp. 555-558,
ISBN: 978-989-96219-1-6.
3. Cruzeiro C, Rema, P, Peixoto, F, Pires, M, Afonso, A. 2009. The anti-phospholipase effects at
muscle lipid oxidation level: histological and immunological parameters when applied to
rainbow trout (Onchorhynchus mykiss) feed. In: Livro de Comunicações do XVIII Congresso de
Zootecnia and II Congresso Ibero-Americano de Zootecnia, 6-9 Maio 2009, Vila Real, Portugal,
pp. 564-568, ISBN: 978-989-96219-1-6.
4. Katia, P, Rema, P, Peixoto, F, Dias, J. 2009. Dietary Hemp By-products as alternative dietary
sources in juvenile turbot (Scophtalmus maximus). In: Livro de Comunicações do XVIII Congresso
de Zootecnia and II Congresso Ibero-Americano de Zootecnia, 6-9 Maio 2009, Vila Real, Portugal,
pp. 560-563, ISBN: 978-989-96219-1-6.
5. Monteiro, JC, Vaz-Pires, P, Barros, RM. (in press). Avaliação do grau de conformidade da
rotulagem de géneros alimentícios de origem animal face à legislação nacional e comunitária em
vigor. Revista Portuguesa de Ciências Veterinárias.
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MASTER THESES
Borges, PAC. 2009. Optimização do nível lipídico em dietas para juvenis de linguado. Master
thesis in Quality Control - water and sediments specialization, Faculty of Pharmacy, University of
Porto. Supervisor: Beatriz Oliveira (FFUP), Co-supervisor: L Valente.
(UTAD). Supervisor: Paulo Rema, Co-supervisor: J Dias.
Escorcio Pereira, CF. 2009. Comparative performance of growth, carcass and biochemical
composition in diploid and triploid rainbow trout (Oncorhynchus mykiss) fed different L-carnitine
levels. Master thesis, ICBAS, University of Porto. Supervisor: R Ozório.
University of Trás-os-Montes and Alto Douro (UTAD) and ICBAS, Uiversity of Porto. Supervisor:
Paulo Rema, Co-supervisor: JF Gonçalves.
alimentar de juvenis de rodovalho Scophtalmus maximus. Master thesis, University of Trás-osMontes and Alto Douro (UTAD). Supervisor: P Rema, Co-supervisor: J Dias.
PhD THESES
Seixas, P. 2009. Composición bioquímica y crecimento de paralarvas de pulpo (Octopus vulgaris
Cuvier, 1797), alimentadas com juveniles de Artemia enriquecidos com microalgas y otros
suplementos nutricionales. PhD thesis in Aquatic Sciences, ICBAS, University of Porto.
Supervisor: Manuel Rey Mèndez (Universidade de Santiago de Compostela), Co-supervisor: Ana
Otero (Univ Santiago de Compostela), L Valente.
Silva, ACF. 2009. Regulação Nutricional da Deposição lipídica em goráz (Pagellus bogaraveo).
PhD in Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Valente, Co-supervisor:
Geneviéve Corraze (Station de Recherches en Hydrobiologie, INRA, France). (Submitted in 2009,
defense in January 2010).
Silva, JMG. 2009. Effectiveness of alternative protein sources in meeting the true amino acid
requirements of Senegalese sole. PhD in Aquatic Sciences, ICBAS, University of Porto.
Supervisor: L Valente, Co-supervisors: Marite Espe (NIFES, Norway), L Conceição.
Rocha, Co-supervisor: L Valente. (Submitted in 2009, defense in January 2010).
273
Collaboration protocol with a feed and aquaculture company A. Coelho e Castro, for the
development of dietary formulations for Senegalese sole, reduction of the environmental
impact and assurance of fish quality (Project QREN, Support to investigation of applied research
in companies, 2009-2012).
LANUCE together with ICBAS/UP coordinate the ECOPISCIS project in consortium with a
Portuguese aquaculture enterprise Quinta-do-Salmão Ltd. (Project QREN 3442). The aim of
ECOPISCIS is to develop an environmental friendly, long-run alternative to minimize biological
waste from fish production.
LANUCE together with the Aquaculture research group (CCMAR) coordinate the OPTIDIETAS
project in consortium with a feed company SORGAL, S.A. (Project IDEIA 70/00073, Support to
investigation of applied research in companies). This project is focused on the optimization of
dietary formulations and feeding practices for Senegalese sole and blackspot seabream, based
on the specific nutritional requirements and feeding behaviour of each species.
LANUCE has long established collaborations with several international Institutes to develop
research programs and co-supervision of PhD students:
-
SEACASE, financed by the EU, involving 5 Countries, coordinated by CCMAR.
-
NIFES, Bergen, Norway. Co-supervision of 1 PhD thesis (Marit Espe): 3 publications.
-
UMR, Nutrition Aquaculture Génomique, INRA, St-Pee-Sur Nivelle, France. Cosupervision of a PhD thesis by Geneviéve Corraze: 4 publications. 1 COST action 825.
-
Bødo University, Norway. Co-supervision of a PhD thesis by Jorge Fernandes. 1 COST
action 825, 1 proposal for a PhD thesis.
-
NIOZ; Royal Netherlands Institute for Sea Research, The Netherlands. Co-supervision of
1 MSc thesis (CC de Aguiar Carvalho).
-
Barcelona University, Spain (Joaquin Gutierrez). 2 publications. 1 COST action 825.
-
Instituto Español de Oceanografia and Consellería de Pesca e Asuntos Marítimos
(CPAM), Spain (Peleteiro, T, Olmedo, M, Linares, F, Villanueva, JLR). 1 Interreg project
submitted and 1 Spanish project. 2 publications.
-
University of Santiago de Compostela (USC), Spain. Co-supervision of 1 PhD thesis
(Manuel Rey Mèndez and Ana Otero). 4 publications.
274
-
INPA, National Institute for Research in the Amazon, Brazil (Manoel Pereira); UFAC and
Universidade Federal do Acre (Ricardo Amaral), Brazil. 1 PhD thesis.
-
Iran’s International Sturgeon Research Institute, Rasht, Iran. 1 publication (Mohseni, M,
Ozorio, ROA, Pourkazemi,M, Bai, SC. 2008. Journal of Applied Ichthyology 24:646-649).
LANUCE participated in the meetings promoted by the North Regional Coordination and
8e. Future research
3. Objectives
In the Division of Aquaculture & Marine Biotechnology of CIMAR-LA, there are several groups
specifically dedicated to the research line Aquaculture. The objective of LANUCE is to contribute
to the implementation and consolidation of Fish Nutrition through a more thigh collaboration
with those groups. LANUCE and AQUAGROUP have currently joint PhD projects with the
participation of international groups. These co-supervised projects were elaborated in order to
optimize the expertise of each group and to guaranty the student the best institutional facilities
to develop the proposed research line in the due time.
In specific terms, the central aim of LANUCE still is contributing to the sustainable development
of the aquaculture industry. Plant protein ingredients will be considered for dietary
incorporation.
The effects of such diets on fish growth and on the final muscle quality will be evaluated. The
optimization of feeding regimes and rearing conditions of Senegalese sole will be a priority in
collaboration with Aquagroup from CCMAR. Large-scale experiments will be considered to
validate our laboratory-scale results. These are applied research lines that we expect to develop
further in collaboration with fish farmers from Portugal and North Spain.
Particular attention will be given to the development of new tools to assess muscle growth and
final quality to combine with the classic morphometric approach already implemented in our
laboratory. Understanding the molecular basis of environmental-induced phenotypic plasticity
of muscle development and growth will be a main research line.
LANUCE will reinforce its activity towards the improvement of food quality and security of
fisheries and aquaculture products. The predicted work includes the further development of
sensory schemes based on Quality Index Method, the most recent sensory classification system
for seafood items.
275
In terms of physical methods, new methods for the evaluation of rigor mortis will be developed,
as well as others that proved to be efficient and useful in quality and welfare evaluations, in
addition to the already used instrumental methods (Torrymeter and Freshmeter). In terms of
microbiology, the first studies will be focused on the characterization of the microbiological
environment of freshwater and seawater farms, including animals, water and surfaces, and
eventual resistances to antibiotics (antibioresistances) shown by commensal (Escherichia coli
and Enterococcus spp.) and pathogenic bacteria (Salmonella sp.); their ability to transmit those
resistances to humans directly from the consumption of farmed products and also by the
aquatic environment will also be considered.
Cage aquaculture is a new technology promising high, but large amounts of feed is directly or
indirectly released to the surrounding environment as excreted wastes. In the LANUCE
researchers’ viewpoint, the solution to minimize the environmental impact of the cage
aquaculture can be achieved by improving the aquaculture technology, more particularly by
developing automated and eco-friendly cage aquaculture technology. Thus, LANUCE is carrying
out studies to understand how fish control their feed intake, and to develop tools to increase
waste recovery. In that way, LANUCE expertise may help the aquaculture sector designing feed
and feeding strategies to optimize production, minimizing feed waste.

Current funding
49.970 EUR (CIIMAR), SEACASE, EU FP6-2005-SSP5A. Total funding: 2.391.401 EUR; CIMAR-LA:
312.486 EUR, 2007-2009. Coordinator: MT Dinis (Aquagroup, CCMAR).
188.327 EUR, FCT PTDC/MAR/70858/2006, TEXBREAM - Post-mortem changes in gilthead sea
bream muscle proteins: its implications to flesh texture. 2008-2011. Coordinator: J Dias
(Aquagroup, CCMAR).
40.200 EUR (CIIMAR), QREN, ECOPISCIS - Waste Management in Fish Production. Project 3442.
Total funding: 591.369 EUR, 2009-2011. Coordinators: JF Gonçalves (ICBAS), R Ozório
45.000 EUR (CIIMAR), QREN, PROAMBIENTE, Dietas sustentáveis e de reduzido impacto
ambiental para o linguado senegalês. 2009-2011. Coordinator: L Valente

Pending funding
217.153 EUR, DRP-Acções Colectivas (PROMAR/1198/2006), PROALGA. Coordinator: P Rema.
92.000 EUR, EDP funding for Biodiversity, Conservation of trout populations in the rivers of
North Portugal: importance of the genetic preservation and ecological assessment. Coordinator:
P Rema.
154.383 EUR, FCT PTDC/MAR/099948/2008, PROBIOFISH. Coordinator: R Ozório.
145.334 EUR, FCT PTDC/MAR/099952/2008, MODELFISH. Coordinator: R Ozório.
276
127.873,20 EUR, FCT PTDC/MAR/099996/2008, SOLIPIDS. Coordinator: L Valente.
99.312 EUR, FCT PTDC/MAR/098496/2008, EPISOLE. Coordinator: L Valente
89.304 EUR, FCT PTDC/BIA-BEC/105266/2008, Coordinator: A Correia (Ecophysiology group,
CIIMAR).
107.000 EUR, FCT PTDC/MAR/098038/2008, Stress in estuarine glass eel. Coordinator: J Wilson
(Ecophysiology group, CIIMAR).
199.488 EUR, FCT PTDC/MAR/100209/2008, Diet optimization for zebra sea bream juveniles, a
new species for aquaculture: meeting nutritional requirements. Coordinator: A Oliva-Teles (Fish
Nutrition Group, CIIMAR).
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Maria Luisa Machado Cerqueira Bastos
-
-
European funding
132.900 EUR, Interreg IV POCTEP, Observatorio oceánico del margen ibérico (RAIA)., 01/200912/2011.
43.800 EUR, Era-Net AMPERA-FCT, Detection and Evaluation of Oil Spills by Optical Methods
(DEOSOM), 09/2008-08/2011.
1.200 EUR, COST Action ES0701, 2009.
-
Private funding
60.000 EUR, Administração dos Portos do Douro e Leixões (APDL), Conservação e Análise do
Registo Histórico dos Maregramas de Leixões, 2009-2010.
1. Objectives
During 2009 the group’s research work focused on two main topics – ocean circulation and
coastal processes – using multi-disciplinary and multi-parameter approaches.
In terms of ocean dynamics studies, several complementary aspects were addressed, to better
understand temporal and spatial evolution of meso and sub-mesoscale dynamics in the NE
Atlantic. Island-induced features were studied using a multiplatform approach, i.e. combining
analysis of remote sensing and in situ data with numerical studies, and improvements to coastal
278
ocean models were tested, using: the derived boundary conditions; assimilation of sea-surface
height dynamics (estimated from improved geoid and satellite altimetry data); and remote
sensing data, such as Synthetic Aperture Radar (SAR), to complement in-situ and numerical
modelling data. The ultimate goal of this line of work is to contribute to the improvement of a
NE Atlantic region circulation model, and the implementation of a regional ocean forecasting
system for the North Iberian Peninsula.
The group engaged in tidal modelling, with analysis of historical and actual tide gauge data
towards the evaluation and understanding of long term sea-level variations along the
Portuguese coast, including reliability assessment. A good understanding of sea level changes is
not only important for global change studies but is also essential for the study of coastal
processes. Therefore, in addition to global mean sea level research we also focus on regional sea
level. These two scales are investigated using several tools and data: tide models; tide gauge sea
level records (a series of 100 years of records is being digitised and analysed); geodetic
information from GPS and gravity measurements (to monitor and understand land movement
and the effects caused by the redistribution of water masses). The combination of geodetic and
physical oceanography is being explored, in particular towards the development of a tidal model
for the Portuguese coast.
The group continued its work on the understanding of coastal and estuarine dynamics.
Environmental monitoring was based on different assessment tools: exploitation of Mobile
Mapping techniques (with videogrametry) for coastal monitoring towards the morphodynamic
characterization of sandy littorals and the assessment of sediments transfer; and, exploitation of
GNSS/INS techniques integrated with laser systems for the implementation of a waterborne
system that will allow an efficient water pollution (by accidental or criminal oil spillage)
surveillance, including real-time georeferencing capabilities.
Other objectives of the group in 2009 were to:
-
Complete and stabilize the implementation of a high performance computing system
(HPC) at CIIMAR to enable numerical modelling studies;
-
Participate in the training of graduates at the Master and PhD levels, both nationally and
internationally, including collaboration in doctoral programmes;
-
Prepare and submit applications to national and international funding opportunities;
-
Deliver results in the scope of current, national and international, research project
agendas;
-
Cooperate with other research groups, supporting (geographical) data analysis and the
use of remote sensing data;
-
Participate in the LA initiatives for the dissemination of scientific results and public
outreach.
279
Main achievements of the research team during 2009 were:
-
The setup of the CIIMAR high performance computing system (HPC). This system, being
fully controlled by COD, has enabled the group to get involved in numerical applications
of ocean circulation models at more ambitious levels and to offer a series of
independent services, such as a database of satellite data, which is currently available
online at: http://frontend-b.ciimar.up.pt/las. This database will soon host datasets from
other research groups (CESAM –University of Aveiro).
-
The development of software packages to model the ocean tides and to compute the
deformation of the Earth due to loading, considering different elastic properties
structures of the Earth.
-
Continuation of the improvement of the algorithms for GPS time-series analysis, aiming
at the detection and interpretation of seasonal signals. These algorithms can also be
applied to other types of time-series.
-
The recovery of 100 years of tide gauge records into a digital format, allowing analysis of
this unique dataset towards a better understanding of long term sea-level changes at
the Portuguese coast.
-
Continuation of numerical studies of the island wakes of the Madeira Arquipelago,
following the approval of the ESA Cat 1 research project (C1-6248) to use Synthetic
Aperture Radar (SAR) data to study time and spatial evolution of meso and submesoscale island-induced features in this NE Atlantic region, combining remote sensing
and in situ data analysis with numerical studies. In situ data were collected during two
research cruises in the summer of 2009 and two ARGOS buoys were launched and
monitored for 6 months. The use of SAR in Madeira has proven advantageous with
respect to overcoming the difficulty in collecting cloud free data as well as the capability
of near-shore resolution (near-field).
-
Acquisition of new data in the scope of the sandy littorals monitoring program, together
with the integration of data acquired with Mobile Mapping Technologies and airborne
imagery data to build Digital Terrain Models (DTM) for the characterization of the
morphodynamic evolution of sandy littorals in particular and coastal erosion studies in
general.
Other actions that involved COD members were:
-
Collaboration in the joint Doctoral School in Marine and Environment Sciences, between
the University of Porto and the University of Aveiro. A member of our team was a guest
lecturer in the Master program in Oceanography in the University of Las Palmas, Gran
Canaria.
-
Participation in the “Ecological Modelling and Coastal Management” subject of the
ICBAS-UP Master in Marine Sciences - Marine Resources; lecturing.
280
-
Preparation of joint projects applications to different national and international funding
opportunities such as: FCT/(PTDC/MAR; Programa Operacional Regional do Norte
(ON.2); European EUFAR (http://www.eufar.net/) FP7 initiative; NRL - Naval Research
Laboratory of the US Navy.
The strengthening of the collaboration with other research groups, inside and outside CIIMAR,
was also a concern. In particular we refer the collaboration with:
-
Universities of Lisbon and of Madeira for the study of ocean and atmospheric gravity
waves and research into local to meso-scale features around the Madeira Islands;
-
University of Azores (LAMTEC) for joint supervision of PhD research;
-
University of Minho in the study of coastal morphodynamic changes through monitoring
programs;
-
Hydrographic Institute in the study of river-ocean interactions and the transfer
processes between estuaries and the shelf;
-
CIIMAR Biodiversity of Aquatic Ecosystems group in the characterization of river habitat
and water quality, and the assessment of shrimp and fish population dynamics.
The strengthening of the links with local authorities and private companies towards the
application of research results was pursued. Examples are: digitalization and analysis of
historical tide-gauge data with the Administração dos Portos do Douro e Leixões (APDL);
modelling of sea bird distributions for the establishment of Important Bird Areas (IBA) with the
Sociedade para o Estudo das Aves (SPEA); coordination of the protocol between the Parque
Biológico de Gaia and the Minho University concerning the Erosion Risk Assessment of the Gaia
Littoral; and, partners of the protocol between the Minho University and Acuinova - Actividades
Piscícolas SA, concerning the Environmental Monitoring of a turbot growing aquaculture project
in Mira.
Dissemination activities included the presentation of preliminary and final results of our
research at scientific meetings and publishing in proceedings of international conferences, as
well as in peer-reviewed journals.
8d. Productivity
1. Farrell, EJ, Granja, HM, Cappietti, L, Ellis, JT, Li, B, Sherman, DJ. 2009. Wave transformation
across a rock platform, Belinho, Portugal. Journal of Coastal Research SI 56: 44-48. IF=1.366.
and distribution of juvenile flounder, Platichthys flesus, in Minho estuary (NW Iberian
Peninsula). Aquatic Ecology 43(4): 1143-1153. IF=1.549.
281
3. Li, B, Granja, HM, Farrell, EJ, Ellis, JT, Sherman, DJ. 2009. Aeolian saltation at Esposende
Beach, Portugal. Journal of Coastal Research SI 56: 327-331. IF=1.366.
In press
4. Bos, MS, Bastos L, Fernandes, RMS. (in press). The influence of seasonal signals on the
estimation of the tectonic motion in short continuous GPS time-series. Journal of Geodynamics.
Doi: 10.1016/j.jog.2009.10.005. IF=1.812. (Online 2009, printed version April 2010)
5. Granja, H., Rocha, F., Matias, M, Moura, R, Caldas, F, Marques, J, Tareco, H (in press). Lagoa
da Apúlia: a residual lagoon from Late Holocene (NW coastal zone of Portugal). Quaternary
International. Doi: 10.1016/j.quaint.2009.11.004I. F=1.601. (Online 2009, printed version 2010)
6. Sousa, JJ, Ruiz, AM, Hanssen, RF, Perski, Z, Bastos, L, Gil, AJ, Galindo-Zaldívar, J. 2009. PSInSAR processing methodologies in the detection of field surface deformation - study of the
Granada basin (Central Betic Cordilleras, Southern Spain). Journal of Geodynamics. Doi:
10.1016/j.jog.2009.12.002. IF=1.812. (Online 2009, printed version April 2010)
proceedings)
1. Bio, A, Couto, A, Costa, R, Prestes, A, Vieira, N, Valente, A, Azevedo, J. 2009. Biomanipulation
of Furnas Lake, Azores: effects of repeated fish removal. Verhandlungen Internationale
Vereinigung für Theoretische und Angewandte Limnologie 30(8): 1207-1209.
2. Fernandes, RMS, Msemwa, J, Bos, M, and the KILI2008 Team. 2009. The KILI2008 project:
Precise measurement of the highest elevation of Africa: Mt. Kilimanjaro. Geophysical Research
Abstracts, Vol. 11, EGU2009-11274, European Geosciences Union General Assembly, Austria.
3. Honrado, J, Vicente, J, Lomba, A, Alves, P, Macedo, JA, Henriques, R, Granja, H, Caldas, FB.
2009. Fine-scale patterns of vegetation assembly in the monitoring of changes in coastal sanddune landscapes. Web Ecology: http://www.oikos.ekol.lu.se/wejrnl.html.
4. Macedo, JA, Alves, P, Lomba, A, Vicente, J, Henriques, R, Granja, H, Honrado, J. 2009.On the
interest of plant functional classifications to study community-level effects of increased
disturbance on coastal dune vegetation. Acta Botanica Gallica: http://www.bium.univparis5.fr/sbf/.
proceedings)
282
MASTER THESES
Magalhães, A. 2009. Análise da qualidade de ortofotomapas, modelo numérico altimétrico e
cartografia a grandes escalas. Master thesis in Geographic Engineering, Faculty of Sciences,
University of Porto. Supervisor: L Bastos.
Gaspar Meirinho, AI. 2009. Distribuição de alcatraz (Morus bassanus) na costa continental
portuguesa e sua relação com variáveis ambientais. Master thesis in Ecology, Management and
Modelling of Marine Resources, Faculty of Science and Technology, New University of Lisbon.
Supervisor: A Bio, Co-supervisor: João Gomes Ferreira (FCT/UNL).
PhD THESES
Moreira de Sousa, JJ. 2009. Potential of integrating psi methodologies in the detection of surface
deformation. PhD thesis in Surveying Engineering, Faculty of Sciences, University of Porto.
Supervisor: L Bastos, Co-supervisor: Ramon Hanssen (TUD, Delft). (Thesis submitted in 2009,
defense in 2010)
-
Workshop: Douro and Minho - Estuaries and Costal Zone, 16-17 April, Porto, Portugal.
-
VI Conferência Nacional de Cartografia e Geodesia - CNCG2009, May 2009, Portugal.
Member of the Scientific Committee.
-
6º Simposio sobre el Margen Ibérico Atlántico, December 2009, Oviedo, Spain. Member
of the Scientific Committee.
Members of COD are developing the Project: “Conservação e Análise do Registo Histórico dos
Maregramas de Leixões” under contract with the Administração dos Portos do Douro e Leixões
(APDL, Port Authority) to analyse more than 100 years of tide gauge data from Leixões.
Participation in the Management Committee Meeting of the COST Action ES0701
(http://www.cost-es0701.gcparks.com/).
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Collaboration with:
-
The École Polytechnic, Paris, continuing the study of island wake asymmetries with
processing of laboratory physical modelling studies (PIV – Particle Image Velocimetry), in
the scope of the international HYDRALAB-III project.
-
The Onsala Space Observatory, Sweden, in the improvement of the internet ocean tide
loading provider.
-
The University of Newcastle, United Kingdom on the project "Global loading and
deformation at tidal timescales". This project will validate the ocean tides with GPS
observations.
-
The Proudman Oceanographic Laboratory to analyse tidal GPS data.
-
The Instituto de Astronomia y Geodesia (CSIC- UCM), Universidad Complutense de
Madrid to analyse tidal gravity observations made on the Canary Islands.
-
The University of Las Palmas, Gran Canaria for joint supervision of PhD research.
Subcontract work for Faculty of Sciences of University of Porto in the scope of the ESA
COASTALT II project. The work involves the modeling of the ocean tides for the Portuguese coast
(CIIMAR budget 7.500 EUR).
ESA Project C1P6248: Fate of island induced (sub)mesoscale oceanic features in the NE Atlantic.
OCD participated in the meetings promoted by the North Regional Coordination and
8e. Future research
1. Objectives
Understanding the evolution of coastal areas involves the study of ocean dynamics, both at a
regional and local scale, and sea level change, as well as their interactions with the coastal
environments. The main goal of the COD research team is to foster the development of this type
of integrated studies towards a better comprehension of the dynamics of the Coastal and Ocean
areas through application of innovative observation methodologies and the use of remote
sensing data, as well as the implementation and validation of ocean circulation models, at
regional and local scales, applying data assimilation techniques.
284
Next to understanding coastal and ocean dynamics, we also aim to support coastal and ocean
resources management, providing new data and models, as well as better and more efficient
tools for data analysis and forecasting. The possibility to integrate all available information in a
GIS environment for an efficient visualization and analysis will aid the understanding of past
evolution trends and forecasts of potential future scenarios, giving also an important
contribution to support management decisions and ICZM.
In 2010 new researchers joined the COD group, strengthening and adding new capabilities to
the team in areas of satellite altimetry, SAR oceanography and coastal processes. Exploitation of
the synergies of this multi-disciplinary team is expected to result in significant achievements in
the field of oceanography and coastal studies and in an increased potential for new
developments in marine and ocean process studies, in the NE Atlantic region and associated
coastal areas.
The main research topics to focus on will be:
-
Sea level research at a global and regional scale, based on: tide models; sea level tide
gauge (including 100-year records from Leixões); geodetic information from GPS and
gravity measurements (to monitor and understand land movement and the effects
caused by the redistribution of water masses); and, satellite altimetry and gravity
measurements.
-
Study of the ocean dynamics and sea level change relying on the exploitation of remote
sensing techniques, and in particular satellite altimetry and SAR, and in situ data, (from
tide gauge, buoys equipped with different, coastal surveys, etc.). Exploration of the
access to Near Real Time (NRT) SAR data during in-situ sampling campaigns planned for
2010; strengthen the synergetic (multiplatform) approach in observing more wake, eddy
and internal wave episodes and work towards numerical solutions to further understand
their spatial and temporal dynamics.
-
Studying internal waves using SAR images. Internal Waves can affect primary production
and the vertical and horizontal transport of nutrients and zooplankton which
consequently affect biological processes and ecosystems. Studies of Internal Waves are
also of interest given their influence on sediment resuspension and their effect on heat
and salt fluxes, which contribute significantly to climate change.
-
Assessment of the congeneracy of the Azores and St. Helena currents and their role in
the subtropical Atlantic from satellite altimetry. Study of the dynamics of regional and
local ocean circulations in the Azores. Assessment of the correlations between these
dynamics and the fisheries in the Azores and exploitation of assimilation methods based
on satellite altimetry.
-
Improving the method for the computation of the wet tropospheric correction for
coastal satellite altimetry measurements. The GNSS-derived Path Delay (GPD) approach,
has been developed and implemented in the SW European region. Future work aims to
extend this study to reach the maturity required for an operational and desirable global
implementation, the refinement of the data combination methodology and validation of
the method by inter-comparison with available independent data sources.
285
-
Generation of sea level products for climate studies, pending approval of the ESA ITT
AO/1 6207/09/I-LG project .
-
Study of Madeira wind-induced episodes and their oceanic implications using satellite,
in-situ and air-airborne data, pending the EUFAR project approval.
-
Development of an ocean forecasting system, including the application of data
assimilations techniques such as 4DVAR will continue in the scope of the RAIA project.
-
Continue the development of model validation protocols to establish an oceanographic
forecasting system for the North Iberian Peninsula. Efforts for comparison of laboratory
results with numerical simulations, in order to improve ROMS - Regional Ocean
Modeling System ability to study island wakes and mesoscale processes in the NE
Atlantic.
-
Assessment of present coastal dynamics through: coastal monitoring of land forms and
processes, and their forcing factors; impact evaluation of coastal changes related with
climate change, sea-level rise and sand starvation; vulnerability mapping and risk
assessment; and modelling and prediction of future scenarios. Monitoring programs will
be based on terrestrial and airborne acquisition techniques to define 3D evolution
models of the littoral. Algorithms to efficiently extract 3D surface models from airborne
digital imagery and videogrametric data acquired with a mobile mapping system (MMS)
will be improved to achieve better accuracy in the height component of coastal dynamic
studies. Further developments of the available MMS are foreseen, testing the new
sensors (e.g. use of MEMS, laser ranging device) and improvement of the direct georeferencing system.
-
Development fast and precise methods for measuring positions and create 3D models
for cliff security risk monitoring in coastal areas. Terrestrial photogrammetry and laserscanning techniques will be analysed in order to create a methodology for this kind of
analysis.
-
Past coastal environments and processes will be studied through paleoenvironmental
reconstitution, relating them with sea level, climate and sedimentary changes.
-
Exploitation of GPS based Lagragian drifters will continue for the study of estuarine
dynamics.
-
The group intends to continue and intensify: the training of young MSc and PhD
scientists as well as post-doc researchers; cooperation between the team members
within the CIMAR and with researchers from other national and international; as well as
cooperation with public institutions and private companies.

Current funding
132.900 EUR, Interreg IV, RAIA, 01/2009-12/2011.
286
COST Action ES0701, funds to management committee meetings in 2010.
49.800 EUR, ESA/ESRIN 21201/08/I-LG, COASTALT – Development of Radar Altimetry data
processing in the oceanic coastal zone, Phase 2, 11/2009-01/2011.
230.476 EUR, INTERREG III B Atlantic Space, EASYCO – Collaborative European Atlantic Water
Quality Forecasting System, 02/2009-01/2012.
9.249 EUR, Green Islands Azores - MIT, Evaluation of potential energy of the ocean
Tidal/Currents and Thermal gradients in the Azores region, 01/2010-06/2010. 13.715 EUR,
Green Islands Azores - MIT, Evaluation of wave power potential in the Azores region, 01/201007/2010.
24.407 EUR, Green Islands Azores - MIT, Evaluation of wind energy potential in the Azores
region, 01/2010-07/2010.
7.500 EUR, Sub-contract within the COASTALT 2 ESA project
US NRL, Compare laboratory results with numerical simulations to improve ROMS -Regional
Ocean Modeling System ability to study island wakes and mesoscale processes in general.
EUFAR Project to support airborne data acquisition around Madeira Island.

Pending funding (FCT)
54.260 EUR, ASH - The Azores and St. Helena currents from satellite altimetry: assessing their
congeneracy and role in the subtropical Atlantic.
31.848 EUR, PTDC/MAR/112545/2009, Eddy-Island interactions: a Madeira Island case study.
19.308 EUR, PTDC/MAR/111282/2009, Dynamics of diurnal thermoclines in the wake of islands
80.136 EUR, PTDC/AAC-AMB/111213/2009, MODELFISH - Modeling Feed Intake Regulation as a
Tool for Waste Management in Fish Production.
129.240 EUR, PTDC/AAC-AMB/114106/2009, CFLUX - Carbon fluxes in a near shore
environment: testing the ‘Continental shelf pump hypothesis’.
71.088 EUR, PTDC/MAR/111223/2009, RAP - Responses to Anthropogenic Perturbations:
climatic and nutrient effects on rock pool assemblages.
287
Pathology
Pathology
Jorge Guimaraes da Costa Eiras
Funding in 2009:
-
-
QREN-ADI
11.900 EUR, OPTISOLE.
-
Others
600 EUR, Organization of the course: Scientific illustration – Black and White.
1. Objectives
-
Contribute to the knowledge of fish, molluscs and annelids parasites specially that ones
with greater impact in fisheries, aquaculture and public health.
-
Use of parasites as biological tags for commercially important fish populations.
-
Contribute to the knowledge of Digenea parasite species that infect several hosts
collected in different shallow-water ecosystems.
-
Perform a morpho-anatomic and molecular characterization of the different parasitic
stages allowing their identification.
288
-
Contribute to the ecoparasitological characterization and clarification of digenean life
cycles.
-
Develop a database of Digenea ITS1 sequences.
-
Contribute to the knowledge of bacterial fish diseases.
-
Perform studies on the detection and isolation of bacteriophages from the environment
and their use as prophylactic and therapeutic agents against fish pathogenic bacteria.
-
The study of parasites for black scabbardfish stocks discrimination in Portuguese waters
and the characterization of Anisakis spp. infection and the risk of this infection for
human health.
-
The study of the populational dynamic of flounder parasites.
-
The study of the effects of some parasites on carps from Mozambique.
-
Description of species of Myxozoa and Microsporea from Brazilain fishes, and study of
the host-parasite relationship; revision of the species of Myxobolus from Brazilian fish.
-
The identification of nematode fish parasites from Iraq.
-
Several researches conducted in Trematoda Digenea species, namely in the
identification of parasitic larval stages by morphological and molecular features and
characterization of life cycles.
Results obtained allowed the publication of several papers (29) in international Journals (ISI) and
communications in international (18) and national (11) meetings.
During this period 1 PhD and 4 MSc students performed their research in the Laboratory and
1PhD and 2 MSc theses were completed with success.
8d. Productivity
1. Azevedo, C, Casal, G, Garcia, P, Matos, P, Teles-Grilo, L, Matos, E. 2009. Ultrastructural and
phylogenetic data of Chloromyxum riorajum sp. nov. (Myxozoa), a parasite of the stingray
Rioraja agassizii in Southern Brazil. Diseases of Aquatic Organisms 85: 41-51. IF=1.687.
2. Azevedo, C, Casal, G, Matos, P, Ferreira, I, Matos, E. 2009. Light and electron microscopy of
the spore of Myxobolus heckelii n. sp. (Myxozoa), parasite from the Brazilian fish Centromochlus
heckelii (Teleostei, Auchenipteridae). Journal of Eukaryotic Microbiology 56: 589-593. IF=2.355.
289
3. Azevedo, C, Casal, G, Mendonça, I, Matos, E. 2009. Fine structure of Henneguya hemiodopsis
sp. n. (Myxozoa), a parasite of the gills of the Brazilian teleostean fish Hemiodopsis microlepes
(Hemiodontidae). Memórias do Instituto Oswaldo Cruz 104: 975-979. IF=2.097.
4. Casal, G, Garcia, P, Matos, P, Monteiro, E, Matos, E, Azevedo, C. 2009. Fine structure of
Chloromyxum menticirrhi n. sp. (Myxozoa) infecting the urinary bladder of the marine teleost
Menticirrhus americanus (Sciaenidae) in Southern Brazil. European Journal of Protistology 45:
139-146. IF=1.966.
5. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2009. Morphological and genetical description
of Loma psittaca sp. n. isolated from the Amazonian fish Colomesus psittacus. Parasitology
Research 105: 1261-1271. IF=1.721.
6. Cavaleiro, F, Santos, MJ. 2009. Seasonality of metazoan ectoparasites in marine European
flounder Platichthys flesus (Teleostei: Pleuronectidae). Parasitology 136(8): 855-865. IF=1.607.
7. Costa, G, Santos, MJ, Costa, L, Biscoito, M, Pinheiro de Carvalho, MA, Melo-Moreira, E. 2009.
Helminth parasites from the stomach of conger eel, Conger conger from Madeira Island, Atlantic
Ocean. Journal of Parasitology 95(4): 1013-1015. IF= 1.195.
8. Cruz, C, Saraiva, A, Santos, MJ, Eiras, JC, Ventura, C, Soares, JP, Hermida, M. 2009. Parasitic
infection levels by Anisakis spp. larvae (Nematoda: Anisakidae) in the black scabbardfish
Aphanopus carbo (Osteichthyes: Trichiuridae) from Portuguese waters. Scientia Marina 73(S2):
115-120. IF=1.174.
9. de Montaudouin, X, Thieltges, DW, Gam, M, Krakau, M, Pina, S, Bazairi, H, Dabouineau, L,
Russell-Pinto, F, Jensen KT. 2009. Digenean trematode species in the cockle Cerastoderma edule:
identification key and distribution along the north-eastern Atlantic shoreline. Journal of the
Marine Biological Association of the United Kingdom 89: 543-556. IF=0.995.
10. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2009. Henneguya corruscans n. sp. (Myxozoa,
Myxosporea, Myxobolidae), a parasite of Pseudoplatystoma corruscans (Osteichthyes,
Pimelodidae) from the Paraná River, Brazil: a morphological and morphometric study.
Veterinary Parasitology, 159; 154-158. IF=2.278.
11. Gordo, LS, Baptista, I, Carvalho, L, Costa, V, Cruz, C, Eiras, JC, Farias, I, Figueiredo, I, Loureno,
H, Bordalo-Machado, P, Neves, A, Nunes, ML, Rei, S, Santos, MJ, Saraiva, A, Vieira, AR. 2009.
Stock structure of black scabbardfish (Aphanopus carbo Lowe, 1839) in the southern North East
Atlantic. Scientia Marina 73(S2): 89-101. IF=1.174.
(Bivalvia: Mytilidae). Marine Biology 156: 959-967. IF=1.999.
13. Marques, JF, Santos, MJ, Cabral, HN. 2009. Zoogeographical patterns of flatfish
(Pleuronectiformes) parasites in the northeast Atlantic and the importance of the Portuguese
coast as a transitional area. Scientia Marina 73(3): 449-471. IF=1.174.
290
14. Moravec, F, Saraiva, A, Abdullah, SMA, Bilal, SJ, Rahemo, ZIF. 2009. Observations on
Rhabdochona spp. (Nematoda: Rhabdochonidae) parasitizing cyprinid fishes in Iraq, with a
redescription of Rhabdochona tigridis Rahemo, 1978. Systematic Parasitology 74: 125-135.
IF=0.911.
15. Pina, S, Barandela, T, Santos, MJ, Russell-Pinto, F, Rodrigues, P. 2009. Identification and
description of Bucephalus minimus (Digenea: Bucephalidae) life cycle in Portugal: morphological,
histopathological and molecular data. Journal of Parasitology 95(2): 353-359. IF=1.195.
16. Pina, S, Tajdari, J, Russell-Pinto, F, Rodrigues, P. 2009. Morphological and molecular studies
on life cycle stages of Diphtherostomum brusinae (Digenea: Zoogonidae) from northern
Portugal. Journal of Helminthology 83: 321-331. IF=0.863.
17. Rangel, L, Santos, MJ. 2009. Diopatra neapolitana (Polychaeta: Onuphidae) as a second
intermediate host of Gymnophallus choledochus (Digenea: Gymnophallidae) in the Aveiro
Estuary (Portugal): distribution within the host and histopathology. Journal of Parasitology 95(5):
1233-1236. IF=1.195.
18. Rangel, L, Santos, MJ, Cech, G, Czeckely, C. 2009. Morphology, molecular data and
development of Zschokkela mugilis (Myxosporea, Bivalvulida) in a polychaete alternate host,
Nereis diversicolor. Journal of Parasitology 95(3): 561-569. IF=1.195.
19. Rato, M, Russell-Pinto, F, Barroso, C. 2009. Assessment of digenean parasitism in Nassarius
reticulatus (L.) along the Portuguese coast: evaluation of possible impacts on reproduction and
imposex expression. Journal of Parasitology 95: 327-336. IF=1.195.
20. Santos, MJ, Saraiva, A, Cruz, C, Eiras, JC, Hermida, M, Ventura, C, Soares, JP. 2009. Use of
parasites as biological tags in stock identification of black scabbard fish, Aphanopus carbo Lowe,
1839 (Osteichthyes, Trichiuridae) from Portuguese waters. Scientia Marina 73(S2): 55-62.
IF=1.174.
21. Saraiva, A, Boane, C, Cruz, C. 2009. Effects of gryporhynchid metacestodes (Cestoda:
Cyclophyllidae) in common carp (Cyprinus carpio) from Mozambique. Bulletin of the European
Association of Fish Pathologists 28(4): 139-143. IF=0.513.
22. Saraiva, A, Ramos, MF, Barandela, T, Sousa, JA, Rodrigues, PN. 2009. Cryptosporidium sp.
(Apicomplexa) from cultured turbot Psetta maxima. Bulletin of the European Association of Fish
Pathologists 29 (1): 34-36. IF=0.513.
In press
22. Cavaleiro, FI, Santos, MJ, Ho, J-S. (in press). Caligus musaicus n. sp. (Copepoda: Caligidae)
parasitic on the flounder Platichthys flesus (Linnaeus) off Portugal. Crustaceana. IF=0.507.
23. Eiras, JC, Monteiro, CM, Brasil-Sato, MC. (in press). Myxobolus franciscoi sp. nov. (Myxozoa,
Myxobolidae), a parasite of Prochilodus argenteus (Actynopterygii, Prochilodontidae) from the
Upper São Francisco River, Brazil, with a revision of Myxobolus spp. from South America. Revista
Brasileira de Zoologia. IF=0.436.
291
24. Francisco, C, Almeida, A, Castro, A, Santos, MJ. (in press). Development of a PCR-RFLP
marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus.
Parasitology International. IF= 1.701.
25. Francisco, C, Hermida, M, Santos, MJ. (in press). Parasites and Symbionts from Mytilus
galloprovincialis (Lamark, 1819) (Bivalves: Mytilidae) of the Aveiro Estuary Portugal. Journal of
Parasitology. IF= 1.195.
26. Romano, L, Eiras, JC, Raibenberg, FC, Alvares, M, Saraiva, A, Cruz, C. (in press). A case of
ceroid deposition in the kidney of feral rainbow trout, Oncorhynchus mykiss, in Alicura
impoundment, Argentina. Bulletin of the European Association of Fish Pathologists. IF=0.513.
proceedings)
proceedings)
1. Cavaleiro, F, Santos, MJ. 2009. Seasonal occurrence of metacercariae of the genus
Diplostomum von Nordmann, 1832 in the eyes of the European flounder, Platichthys flesus
(Linnaeus, 1758) (Teleostei: Pleuronectidae), caught of the northwest Portugal. XI Congresso
Ibérico de Parasitologia. Lisboa, Portugal. Acta Parasitológica Portuguesa 16(1/2): 382-383.
2. Cavaleiro, F, Correia, AM, Santos, MJ. 2009. Seasonal occurrence of gastrointestinal
helmithosis in European flounder, Platichthys flesus (Linnaeus, 1758) (Teleostei:
Pleuronectidae), of marine environments of the northwest region of the Portuguese coast. XI
Congresso Ibérico de Parasitologia. Lisboa, Portugal. Acta Parasitológica Portuguesa 16(1/2):
380-381.
3. Cavaleiro, F, Rangel, L, Santos, MJ. 2009. Copepoda ectoparasites of some commercially
important fish species of the Portuguese coast. XI Congresso Ibérico de Parasitologia. Lisboa,
Portugal. Acta Parasitológica Portuguesa 16(1/2): 378-379.
4. Cruz, C, Saraiva, A, Santos, MJ, Eiras, JC, Ventura, C, Soares, JP, Hermida, M. 2009. Parasitose
provocada por larvas de Anisakis spp. (NEMATODA: ANISAKIDAE) no peixe de espada preto
Aphanopus carbo (OSTEICHTHYES: TRICHIURIDAE) em Portugal. XI Congresso Ibérico de
Parasitologia. Lisboa, Portugal.
5. Duarte, D, Francisco, C, Almeida, A, Castro, A, Santos, MJ. 2009. Genetic identification of
Didymobothrium rudolphii (Cestode: Spatheobothriidea) morphotypes “frontal” and “lateral” in
the Sand sole, Solea lascaris (Risso, 1810). XI Congresso Ibérico de Parasitologia. Lisboa,
6. Duarte, D, Santos, MJ. 2009. Parasitism in Talorchestia brito Stebbing, 1891 of the Northern
and Central coast of Portugal. XI Congresso Ibérico de Parasitologia. Lisboa, Portugal. Acta
Parasitológica Portuguesa 16(1/2): 384-385.
292
7. Francisco, CJ, Almeida, A, Castro, AM, Santos, MJ. 2009. Molecular and morphological data of
the Diphtherostomum brusinae (Trematoda: Zoogonidae) infecting Mytilus galloprovincialis from
Aveiro Estuary, Portugal. XI Congresso Ibérico de Parasitologia. Lisboa, Portugal. Acta
Parasitológica Portuguesa 16(3).
8. Francisco, CJ, Almeida, A, Castro, AM, Santos, MJ. 2009. Molecular data of adult worms of
Prosorhynchus crucibulum and Prosorhynchus aculeatus: sensitivity of a PCR method. XI
Congresso Ibérico de Parasitologia. Lisboa, Portugal. Acta Parasitológica Portuguesa, 16(3).
9. Francisco, CJ, Hermida, MA, Santos, MJ. 2009. It is the miracidium of Prosorhynchus
crucibulum a passively infecting larva? XI Congresso Ibérico de Parasitologia. Lisboa, Portugal.
Acta Parasitológica Portuguesa 16(3).
10. Francisco, CJ, Hermida, MA, Santos, MJ. 2009. Survey of parasites from Mytilus
galloprovincialis of the Aveiro Estuary, Portugal. XI Congresso Ibérico de Parasitologia. Lisboa,
Portugal. Acta Parasitológica Portuguesa 16(3).
11. Poinar, G, Duarte, D, Santos, MJ. 2009. A new parasitic species of Talorchestia brito
(Crustacea: Talitridae) in Portugal: Halomonhystera parasitica Poinar, Duarte and Santos, in
press (Nematoda: Monhysteridae). XI Congresso Ibérico de Parasitologia. Lisboa, Portugal. Acta
Parasitológica Portuguesa 16(1/2): 386-387.
MASTER THESES
Araújo, A. 2009. Presença de Legionella sp. em redes de distribuição hospitalar e sua
Porto), Co-supervisor: JA Sousa.
Reis, R. 2009. Pesquisa de bactérias patogénicas numa piscicultura do norte de Portugal. Master
thesis in Biology and Management of Water, Faculty of Sciences, University of Porto. Supervisor:
JA Sousa.
PhD THESES
Casal, G. 2009. Microsporidioses e Mixosporidioses da Ictiofauna Portuguesa e Brasileira:
caracterização ultrastrutural e filogenética. PhD thesis in Biomedical Sciences, ICBAS, University
of Porto. Supervisors: J Eiras and ML Teles Grilo.
293
Collaboration with institutions from different countries:
-
Univ. Kingston, UK (Prof. Angela Davis).
-
Natural History Museum, London, UK (Prof. David Gibson, Dr. Rod Bray).
-
Czech Academy of Sciences, Czech Republic (Prof. F Moravec, Prof. T Scholz).
-
Hungarian Academy of Science, Hungary (Prof. K Molnár, Prof. C Szekély).
-
Univ. Santiago de Compostela, Fac. Biologia, Dep. Microbilogia y Parasitologia, Spain
(Prof. Alicia Toranzo, Prof. Juan Barja).
-
Instituto de Investigaciones Marinas, Vigo, Spain (Dr. António Figueras, Dr. Camiño
Gesta).
-
Centro de Control do Medio Mariño (CCMM), Consellería de Pesca e Asuntos Marítimos,
Spain (Dr. Jaime Montes).
-
Instituto Nacional de Investigação Pesqueira de Luanda, Angola (Ana Paula, Nelson
Samuel).
-
California State Univ. Long Beach, USA (Prof. Jo-shey Ho).
-
Oregon State Univ., Depart. Zoology, Corvallis, USA (Prof. George Poinar)
-
Humboldt State Univ., California, USA (Dr. Katie R. McGourty).
-
Univ. Maringá, Brazil (Prof. Gilberto Pavanelli, Prof. Ricardo Takemoto).
-
Univ. Rio Grande, Brazil (Prof. Joaber).
-
Fisheries Institute of São Paulo, Brazil (Prof. Maria José Ranzani).
-
Carlos Azevedo Research Laboratory, Federal Rural University of Amazónia, Belém,
Brazil (Prof. Dr. Edilson Matos).
-
Federal Univ. Pará, Belém, Brazil (Prof. Patrícia Matos).
-
Federal Univ. Pernambuco, Recife, Brazil (Prof. Dr. Paulo Padovan, Prof. Dr. Isaíras
Padovan).
-
Federal Univ. Piauí, Fac. Med. Veterinária,Teresina, Brazil (Prof. Ivete Mendonça).
294
-
Federal Univ. Santa Catarina, Florianópolis, Brazil (Prof. Patrícia Garcia).
-
Univ. Federal Fluminense, Fac. Med. Veterinária,Niterói, Brazil (Prof. Dr. Sérgio
Carmona, Prof. Dr. Marcelo Knoff).
-
Univ. Federal Rural do Rio de Janeiro (Prof. Dr. José Luis Luque).
-
Enbrapa, Corumbá, Mato Grosso do Sul, Brazil (Dr. Débora Marques).
-
Univ. Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales,
Departamento de Biología, Laboratorio de Parasitología, Mar del Plata, Argentina (Prof.
Juan Timi).
Collaboration in the project “Revisión de la familia Gymnophallidae Odhner, 1900 basada en
estudios morfológicos y moleculares” with Florencia Cremonte, Centro Nacional Patagónico,
Provincia del Chubut, Argentina.
Evaluation of PhD thesis “Studies on Monogenetic Trematodes of some fishes of Karachi coast”
of the Jinnah Univ. for Women, Nazimabad, Karachi, Pakistan (MJ Santos).
Portuguese contact of the International Ichthyoparasitology Newsletter (MJ Santos).
8e. Future research
1. Objectives
For the next years, the major goals proposed for this group are:
-
The study parasites of the Phyla Microsporidia, Apicomplexa and Haplosporidia in
freshwater and marine animals, such as fishes, crustaceans and molluscs from IberoAtlantic and Brazilian fauna.
-
The study of Myxozoan fish parasites, the host-parasite relationship and the life cycle.
-
Study the economic and commercial impact of parasites in fish and mollusks from
Portugal and Brazil.
-
The search and identification of Digenea parasites from hosts collected in several
shallow-water ecosystems and perform the characterization of their larval stages
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morpho-anatomically, by light and scanning electron microscopy and with the use of
molecular data. Clarify more digenean life cycles. Study the effects that these parasites
exert on their hosts and its influence in the ecosystems dynamics. Study spatial
heterogeneity (based on molecular methods) between sites (local parasite faunas) and
within sites (individual parasite faunas). Increase the digenean species molecular
database and perform phylogenetic studies.
-
The detection and isolation of bacteria and bacteriophages from the environment and
their use as prophylactic and therapeutic agents against fish pathogenic bacteria.
It is also an objective of the group to intensify international cooperation with several institutions
from different countries including with the King Saud University of Riyadh, Saud Arabia.

Current funding
9.500 EUR, QREN FEDER, OPTISOLE.

59.400 EUR, FCT PTDC/BIA-BIC/110130/2009.
200.000 EUR, FCT PTDC/CVT/113045/2009, 2010-2012.
130.000 EUR, FCT PTDC/MAR/112782/2009, 2010-2012.
187.990 EUR, FCT PTDC/MAR/110782/2009.
260.000 EUR, EU FP7-Ocean-2010 – BAPE.
1.000 EUR, technical assistance to fish farms.
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1. Group nName / denomination
Cymon John Cox
1. Objectives
The main objectives were:
-
The establishment of the Plant Systematics and Bioinformatics Research group;
-
To obtaining funding through the FCT Research and Development open call;
-
To establish the computational resources necessary to carry out bioinformatic research.
Two research and development applications were submitted to FCT in January 2009 - PTDC/BIABCM/099565/2008 and PTDC/BIA-BCM/102395/2008. The project PTDC/BIA-BCM/099565/2008
- The origin and early diversification of plants: a phylogenomic approach employing novel
composition-heterogeneous methods, was recommended for funding.
The FCT proposal SFRH/BD/60873/2009 submitted by the applicant Cristiane Cassiolato Pires
Hardoim, and co-supervised by C Cox, was approved.
The FCT proposal SFRH/BPD/48206/2008 submitted by the applicant Natércia Maria da Silva
Conceição, and co-supervised by C Cox, was approved.
297
A small computational computing cluster was purchased and installed in the University of the
Algarve Datacentre. The equipment is administered by Cox, and was purchased from funds
provided by CCMAR (“start-up funding”). The cluster resource is currently utilised by the PSB
group and three other CCMAR reasearch groups for bioinformatics, including phylogenetic
analyses and next-generation sequence assembly and analysis.
8d. Productivity
1. Waeschenbach, A, Cox, CJ, Littlewood, DTJ, Porter, JS, Taylor, PD. 2009. First molecular
estimate of cyclostome bryozoan phylogeny confirms extensive homoplasy among skeletal
characters used in traditional taxonomy. Molecular Phylogenetics and Evolution 52: 241-251.
IF=3.556.
2. Foster, PG, Cox CJ, Embley, TM. 2009. The primary divisions of Life: a phylogenomic approach
employing composition-heterogeneous methods. Philosophical Transactions of the Royal
Society (B) 364: 2197-2207. IF=5.117.
3. Cock, PJA, Antao, T, Chang, JT, Chapman, BA, Cox, CJ, Dalke, A, Friedberg, I, Hamelryck, T,
Kauff, F, Wilczynski, B, de Hoon, MJL. 2009. Biopython: freely available Python tools for
computational molecular biology and bioinformatics. Bioinformatics 25: 1422-1423. IF=4.926.
proceedings)
proceedings)
298
The three publications in peer review journals (Waeschenbach, A et al. 2009. Molecular
Phylogenetics and Evolution 52: 241-251; Foster, PG et al. 2009. Philosophical Transactions of
the Royal Society (B) 364: 2197-2207; Cock PJA et al. 2009. Bioinformatics 25: 1422-1423) were
all the result of international collaboration. C Cox has ongoing international collaborative
projects with J Shaw, Duke University, North Carolina, USA, P Foster (Natural History Museum,
London, UK) and M Embley, (Newcastle Universtiy, Newcastle, UK).
8e. Future research
1. Objectives
To initiate the FCT funded project PTDC/BIA-BCM/099565/2008, a Post-doctoral Research
Assistant will be sought. The candidate will be sought internationally and be required to have
previous experience at PhD level in phylogenetics, bioinformatics, and computer programming.
A project coordination meeting will occur at the end of April 2010 with international
collaborators from the UK, after the Post-doctoral Research Assistant has taken up the position
at CCMAR. The project PI and Post-doctoral Research Assistant will present preliminary work on
the project and an international meeting during the summer of 2010. The computation
resources available to the group will be expanded as part of the project PTDC/BIABCM/099565/2008, and software installation and analyses piplines will be constructed to
analyse data from Roche 454 and Illumina/Solexa short-read technologies, and the RSeq-CHiP
technique.
Additional funding will be sought from the FCT during the annual R&D open call during 2010 to
continue phylogenomic analysis of the green algae and land plants. Funding for laboratorybased phylogenetic data collection (PCR, DNA sequencing) and 2nd generation sequencing
technologies.

Current funding
104.265 EUR, FCT PTDC/BIA-BCM/099565/2008, The origin and early diversification of plants: a
phylogenomic approach employing novel composition-heterogeneous methods. 01/201012/2012.
299

Pending funding
155.528 EUR, FCT PTDC/BIA-EVF/113129/2009, A conquista do meio terrestre: estudo
filogenético da evolução do cloroplasto das Charophyta e plantas terrestres utilizando modelos
de substituição heterogéneos de dados e tempo.
300
Synthesis and Organic Reactivity
4. Group nName / denomination
Group of Synthesis and Organic Reactivity
Maria Lurdes Santos Cristiano
-
-
National funding
20.400 EUR, FCT PTDC/QUI/67674/2006, Studies on the reactivity of derivatives of tetrazole and
isothiazole: isothizazolyl-tetrazoles as potential bidentate ligands and their application in
catalysis. 2007-2010.
172.480 EUR, FP7 ARTEMIP, The safety pharmacology of Artemisinins when used to reverse
pathophysiology of Malaria in Pregnancy. 2008-2011.
1. Objectives
The research in the group is directed to two main areas: (i) Organic Reactivity and (ii) Medicinal
Chemistry.
(i) Organic Reactivity
-
The investigation of structure-reactivity relationships is essential for the interpretation
and prediction of the outcome of reactions. This general methodology may be applied
to all reacting systems, and is crucial for the development of major areas. For instance, it
establishes grounds for the elucidation and interpretation of the interactions between
pharmacologically active compounds and their biological targets, providing essential
information for drug design and optimization.
301
-
Research activities in the SOR group have been mostly directed to the field of Reactivity,
within the broad research area of Physical Organic Chemistry. The work performed
involves the synthesis, structural analysis and investigation of the reactivity of
derivatives of heterocyclic bioactive compounds. The aim is the improvement and finetuning of properties, for instance the optimization of biological activity, and also the
development of new synthetic methods applicable to the preparation of bioactive
compounds and to the modification of natural products. Structural elucidation and
mechanistic investigations are approached, in catalysed and non-catalysed reactions.
These include: (i) reductive cleavage of carbon-oxygen bonds catalysed by transition
metals, (ii) sigmatropic isomerisations in allyl vinyl ethers, (iii) photochemistry and
thermochemistry of bioactive derivatives of tetrazole and benzisothiazole, (iv)
thermolysis and photolysis of carbamates.
(ii) Medicinal Chemistry
-
During the last years, the SOR group has been researching in the field of Medicinal
Chemistry. Within this area we aim at the development of synthetic routes to DNAdirected endoperoxides. These hybrid targets combine a polypyrrole-type linker bearing
DNA binding properties, with an endoperoxide pharmacophore. The rationale is based
on the ability of Fe(II) to selectively cleave the peroxide bridge of peroxide-type drugs,
generating radical species (ROS or carbon-centred radicals) capable of damaging key
biomolecules. In this case, the drug target is DNA. Thus, the active species produced
should be capable of simultaneous selective oxidative damage to a complementary
strand of DNA and non-covalent interaction to the helix, in a combination
chemotherapy-like approach. Because tumour cells are known to have higher Fe(II)
concentrations than normal cells, the DNA-directed peroxide compounds designed by
the group may also act as potential antiproliferative agents in various cancer cell lines.
In fact, several artemisinin-type compounds have shown cytotoxic activity in the low
micromolar range against various tumour cell lines.
-
In vitro and in vivo tests for our compounds are conducted at the Liverpool School of
Tropical Medicine and at the Department of Pharmacology of the University of
Liverpool. These institutions are our partners in this project.
-
Within ARTEMIP, the aim is to investigate the embriotoxicity and teratogenicity of
Artemisinin-type drugs. The partner institutions are The Liverpool School of Tropical
Medicine, the University of Liverpool, the University, Gotenborg, the University of
Leiden and WHO/TDR. The work packages under the responsibility of the University of
Algarve are related with the synthesis of probe peroxides with demonstrated
antimalarial activity (affinity labelled, radio-labbeled or fluorescent) in order to assess
distribution and accumulation and elucidate the mechanism of action.
Under the research topic of reactivity, research in the area of Physical Organic Chemistry was
continued. The studies were directed to the investigation of structure and reactivity of bioactive
derivatives of tetrazole and benzisothiazole (saccharin).
302
An important investment was made in the design and synthesis of endoperoxide-based
antimalarials and falcipain inhibitors. The targets are hybrid drugs, designed within the concept
of combination chemotherapy, whereby two active pharmacophores, directed to different
intracellular targets, are combined within the same chemical entity.
A major effort has been made towards meeting the objectives of the ARTEMIP project, started
in March 2008. The first year review meeting was held in Faro, and was very successful. In
accordance with the initial specifications of the workpackage related to synthesis, the
corresponding deliverables were provided. Results of biological tests on the compounds
delivered have called for the synthesis of other analogues, which is now being done. We aim at
providing a set of probes that will be successfully used to attain the final goal of the ARTEMIP
project.
Collaboration has been established with the EDGE group of CCMAR. The aim of this research is
to evaluate the activity of peroxides known to exhibit antimalarial activity on Perkinsus olseni. A
range of endoperoxide compounds was prepared, and tested on cultures of P. olseni,
Preliminary results show that the compounds act as growth inhibitors of P. olseni in the
micromolar range. Studies were conducted in order to evaluate the effect of Fe(II) concentration
on activity. Results point to a Fe(II) concentration dependent mechanism of activation/growth
inhibition. Further investigation is planned, directed to an evaluation of the mechanisms of
action and accumulation. Some endoperoxides linked to a fluorophore label are being used,
following analysis by confocal microscopy.
8d. Productivity
1. Gómez-Zavaglia, A, Kaczor, A, Coelho, D, Cristiano, MLS, Fausto, R. 2009. Conformational and
structural analysis of 2-allyl-1,2-benzisothiazol-3(2H)-one 1,1-dioxide as probed by matrixisolation spectroscopy and quantum chemical calculations. Journal of Molecular Structure 919:
271-276. IF=1.551.
2. Frija, LMT, Fausto, R, Loureiro, RMS, Cristiano, MLS. 2009. Synthesis and structure of novel
benzisothiazolyl derivatives of tetrazole for potential application as nitrogen ligands. Journal of
Molecular Catalysis A: Chemical 305: 142-146. IF=3.135.
3. Gómez-Zavaglia, A, Kaczor, A, Almeida, R, Cristiano, MLS, Eusébio, MES, Maria, TMR, Mobili,
P, Fausto, R. 2009. The thermally induced sigmatropic isomerization of pseudosaccharyl allylic
ether. The Journal of Physical Chemistry A 113(15): 3517-3522. IF=2.899.
4. Araújo, NCP, Barton, V, Jones, M, Stocks, PA, Ward, SA, Davies, J, Bray, PG, Shone, AE,
Cristiano, MLS, O’Neill, PM. 2009. Semi-synthetic and synthetic 1,2,4-trioxaquines and 1,2,4trioxolaquines: synthesis and preliminary SAR and comparison with acridine endoperoxide
conjugates. Bioorganic and Medicinal Chemistry Letters 19: 2038-2043. IF=2.650.
5. Almeida, R, Ismael, A, Gómez-Zavaglia, A, Kaczor, A, Cristiano, MLS, Fausto, R. 2009. Matrix
isolation FTIR, theoretical structural analysis and reactivity of amino-saccharins: N-(1,1-dioxo-
303
1,2-benzisothiazol-3-yl)-N-methyl
amine
and
N-(1,1-dioxo-1,2-benzisothiazol-3-yl)-N,Ndimethylamine. Journal of Molecular Structure 938: 198-206. IF=1.551.
6. Gomez-Zavaglia, A, Reva, ID, Frija, LMT, Cristiano, MLS, Fausto, R. 2009. Photochemistry of
tetrazole derivatives in cryogenic rare gas matrices. Chemical Physics Research Journal 1(4): 221250. (Review article).
proceedings)
proceedings)
-
ESOR XII, European Symposium or Organic Reactivity, 6-12 September, Haifa, Israel 2009
(Member of the International Advisory Board and chair of a plenary session).
-
9º Encontro Nacional de Química Física da SPQ, 15-16 June, Aveiro, Portugal (Member
of the Scientific Committee).
University of Liverpool, UK, Departments of Chemistry and Pharmacology, Liverpool School of
Tropical Medicine University of Coimbra, Department of Chemistry University of La Plata,
Argentina, Center for Research and Development in Food Cryotechnology (CIDCA) Jagiellonian
University, Poland, Faculty of Chemistry, Ingardena University of Leiden, Netherlands University
of Goteborg, Sweden World Health Organization (WHO/TDR) Geneva Centro de Malária e
Doenças Tropicais.
The publications and on-going projects reflect these collaborations.
304
8e. Future research
1. Objectives
For this year, in the area of medicinal chemistry, the aim is to pursue research leading to the
development of hybrid drugs for malaria chemotherapy, combining an endoperoxide
pharmacophore and a DNA binder with affinity to the G-quadruplexes of the plasmodium
telemeres.
In the area of reactivity, structural studies on derivatives of heterocyclic bioactive compounds
such as tetrazole and benzisothiazole, and investigation of their reactivity, in view of developing
synthetic methodologies, will continue.
Special attention will be devoted to the application of these compounds in the design of
nitrogen ligands applicable in catalysis and in supramolecular chemistry.

Current funding
172.480 EUR, FP7, ARTEMIP, 2008-2011.

119.004 EUR, FCT PTDC/SAU-FCB/103085/2008, A multi-target therapeutic strategy to
overcome the selection of resistance to antimalarial drugs and drug-candidates.
153.398 EUR, FCT PTDC/QUI-QUI/113197/2009, Investigation of the structure, thermal and
photochemical reactivity of endoperoxides with antimalarial activity (PER-REACT).
305
9. OTHER LA ACTIVITIES
9a. Services and resources
1. Internal services and resources (Sharing of equipment and other resources both within
the LA, between LAs or with other institutions of Higher Education nationally or
internationally)
CIMAR LA occupies two distinct poles, one at the Univ Porto (ca 3500 m2) and another one at
the Univ Algarve (ca 1800 m2). Each pole also includes facilities (1000 m2) with water closed
circuits for animal maintenance with an overall capacity of 60000 liters of seawater and
freshwater. CIMAR researchers have also access to an aquaculture experimental station located
in the Algarve (ca 2 ha). CIMAR acquired two small vessels (12 and 17 meters), one was
completely operational for coastal and estuarine campaigns in 2009 and another was being
equipped.
In 2009, the proposal for the new building of CIIMAR, to be constructed within the Park of
Science and Technology of the Univ Porto, was submitted and accepted for funding (ERDF).
In the last years, significant equipment was acquired through the national competitive program
for scientific re-equipment launched by FCT (2 million EUR), mostly for analytical chemistry and
molecular and genetic research (a list of the equipment is presented in the 2008 report).
Through another funding program, an Isotope Ratio Mass Spectrometers (IRMS) was also
acquired. This equipment is shared within CIMAR but is also available to other LAs and Higher
Educations Institutions. CIMAR can also access equipment from other LA and Higher Education
Institutions (e.g. scanning and transmission electron microscopy).
CIMAR High Performance Computer (HPC - 160 CPUs, 360 GB of RAM, 4 TB of storage space,
http://nautilus.ciimar.up.pt), used for parallel computing (e.g. run ocean circulation numerical
models, meteorological forecasting models, etc.), was fully operational in 2009. The North
Iberian Ocean Observatory (RAIA) enables access to updated databases of satellite remote
sensing data and numerical model solutions, for the NE Atlantic. CIMAR HPC is an integral part
of the Univ Porto computer network and there are plans to be an active stakeholder of the
IBERGRID as well as EGEE (http://www.eu-egee.org/).
CIMAR is partner in several initiatives, such as the European Network of Marine Institutes and
Stations - MARS (www.marsnetwork.org), the European Marine Biological Research Centres EMBRC
(www.embrc.eu)
and
the
ASSEMBLE
FP7
infrastructure
project
(www.assemblemarine.org), that facilitate collaborations and shared access to infrastructures
and to a comprehensive set of coastal marine ecosystems and marine organisms, research
vessels and state-of-the-art experimental genomic and proteomic facilities.
Other resources shared with national and international institutions include: cell culture facilities
and cell lines (e.g. Univ Bergen, NIFES, Univ Ghent), zebrafish culture facilities (IMM-Institute for
Molecular Medicine, Lisbon).
In the framework of the agreement between FCT and the Portuguese Hydrographic Institute,
CIMAR researchers have also the possibility to hire ship time at low price.
306
Two services interface have been operating within the LA, for analytical chemistry and
molecular and genetic research. Several services are installed in both centers for administrative
and technical support, with a total of 24 staff members, including public and international
relations, science and technology management, project management, finance, IT systems, image
and communication, juridical, biotery, and maintenance.
Through several protocol agreements with higher education institutions, CIMAR has been
supporting pre and post graduation training by providing facilities and resources including the
participation of non-academic staff in teaching activities.
2. External services and resources (Scientific or technical services provided both nationally
or internationally to Governments/Institutions or the community)
CIMAR has been increasingly committed in the support of public policies. Most relevant
activities to provide scientific and technical services both nationally and internationally to
Governments/Institutions and the community are:
-
Member of European Science Foundation – Marine Board.
-
Founding member of the R&D Consortium OCEANOS, submitted and approved for
funding by FCT in 2009, that aim to reinforce the State Laboratories and provide
scientific basis for advice and policy based on research of excellence on marine science
and technology at an internationally competitive level; join action of 5 State
Laboratories, 1 Associated Laboratory (CIMAR), 4 Universities and 1 R&D network
(http://alfa.fct.mctes.pt/apoios/unidades/consorcios/).
-
Third party of FCT in the ERA-nets AMPERA (www.cid.csic.es/ampera) and Biodiversa
(www.eurobiodiversa.org), FP6.
-
Detachment of a permanent staff element to integrate the Portuguese network of FP7
National Contacts Points, supported by the Minister of Science and Technology, to
provide assistance to potential FP7 participants (Universities, Research Centers, SMEs,
Industry) in all aspects regarding the “Environment, including Climate Change” Theme.
-
Founding member of the Institute for the Development of the Knowledge and the
Economy of the Sea (IDCEM - Agency for the promotion of scientific knowledge,
valorization of research and technology service agreements, and promotion of
entrepreneurship).
-
Member of the Maritime Cluster - OCEANO XXI, through IDCEM, a cluster of enterprises
and research centres that aim to promote and strengthen the maritime economy.
-
Contribution to the implementation of the Ocean Agenda of the North Region, North
Regional Coordination and Development Commission (CCDR-N) (http://www.ccrnorte.pt/pagina.php?p=91); Leadership of the Ocean agenda for the territorial
cooperation North of Portugal - Galicia.
-
Contribution to the Regional Spatial Planning of Algarve, CCDR-Algarve.
307
-
Contribution to the implementation of the Water Framework Directive, National
Institute for Water (INAG) (eg. Project EEMA - Ecological status of coastal and
transitional waters of the Portuguese coast).
Moreover, CIMAR has been involved in several R&D projects aimed to give support to European
Territorial cooperation (eg. Interreg Atlantic Area, Interreg Sudoe, Interreg POCTEP) and has
been regularly requested by the government to give advice on legislation (eg. Environment and
fishing) and bilateral agreements involving Portugal.
At the individual level, members of the Direction of CIMAR have also been involved in several
activities:
-
National delegate for FP7 (“Environment, including Climate Change” Theme).
-
Director of IDCEM and Vice-President of OCEANO XXI.
-
Focal Point of the Minister of Science and Technology on the National Ocean Strategy
(Task Group for Maritime Affairs - EMAM).
-
Members of the Councils of Hydrographic Regions, assisting and advising the
Administrations of the North and Algarve Hydrographic Regions (ARH-Norte, AHRAlgarve).
Moreover, several CIMAR members participate regularly in national (FCT) and international
evaluation panels.
CIMAR provided services for the industry in several areas including water quality (chemical,
biological and toxicological), diet formulation for aquaculture, DNA extraction and genotyping,
physical oceanographic data collection and environmental consulting studies.
Both CIIMAR and CCMAR have been qualified for providing R&D and innovation services to SMEs
under the Portuguese National Strategic Reference Framework (QREN, 2007-2013).
9b. Networking actions
1. Networking actions (Both national and international activities)
Most relevant:
-
Member of European Science Foundation – Marine Board (www.esf.org/researchareas/marine-board.html)
-
EU Networks of Excellence - FP6
MGE - Marine Genomics Europe (www.marine-genomics-europe.org)
MARBEF - Marine Biodiversity and Ecosystem Function (www.marbef.org)
308
-
Marine Research Centres Networks
MARS - European Network of Marine Institutes and Stations (www.marsnetwork.org)
ASSEMBLE - Association of European Marine Biological Laboratories (EU FP7,
www.assemblemarine.org)
EMBRC - European Marine Biological Research Centres (www.embrc.eu)
-
ERA Nets - FP6 (Third party of FCT)
BiodivERsA (www.eurobiodiversa.org)
AMPERA (www.cid.csic.es/ampera)
-
Technological Platforms
European Aquaculture Technology and Innovation Platform (www.eatip.eu)
Water Supply and Sanitation Technology Platform (www.wsstp.eu)
-
ESF Networking Programmes
Functional Dynamics in Complex Chemical and Biological Systems (http://funcdyn.org)
-
COST Actions (www.cost.esf.org)
Improved constrains on models of glacial isostatic adjustment, ES0701
Welfare of fish in European aquaculture - n. 867
Critical success factors for fish larval production in European Aquaculture: a
multidisciplinary network - n. FA0801
-
International Thematic Networks
EPBRS (www.epbrs.org), and the supportive network Biostrat
Ocean Tracking Network (http://oceantrackingnetwork.org)
Seagrass Net Monitoring Program (www.seagrassnet.org)
Historical
Ecology
of
the
(www.biology.duke.edu/corona)
Trans-Atlantic
MedZoo
Network,
through
(www.ciesm.org/marine/programs/zooplankton.htm)
Marine
CIESM
Biota
Network
program
ICES Working Groups on Habitat Mapping, and on Biological Effects of Environmental
Contaminants (www.ices.dk)
309
Red Iberoamericana de Toxicología y Seguridad Química (http://ritsq.org)
-
National Thematic Network
R&D National Consortium OCEANOS
DivMar - National network on Costal Marine Biodiversity (www.cimar.org/divmar)
-
Training Network
European Erasmus Network in Aquaculture, Fisheries & Aquatic Resources Management
(www.aquatnet.com)
-
FP7 National Contact Points Network
CIMAR has detached a permanent staff element to integrate the network of NCP for FP7
(Environment theme)
9c. Training
1. Training activities (Activities of the LA as a whole)
In 2009, 70 MSc students and 24 PhD students completed their degree under (co)-supervision of
CIMAR members (including 3 MSc theses and 6 PhD theses submitted in 2009 to be defended in
2010). New theses are currently underway.
Pre-graduation Studies
-
77 undergraduated students were incorporated in R&D project teams, with Integration
into Research Grants (BII), to foster scientific activity and the development of critical
thinking skills, creativity and autonomy.
-
Additional undergraduated students were involved in the pluridisciplinary program of
Univ. Porto for developing work related with Microbial Life in Extreme Antarctica
Environments.
Post-graduation Studies
-
Doctoral School in Marine and Environmental Sciences, in cooperation with CESAM,
Univ. Porto and Univ. Aveiro; with four areas of specialization: Marine Resources and
Aquaculture, Oceanography and Marine Ecosystems; Environmental Quality;
Environmental Planning and Management.
Involvement in European and National MSc programmes:
-
310
Erasmus Mundus Master in Science in Marine Biodiversity and Conservation
(www.embc.marbef.org)
-
European Master in Aquaculture and Fisheries
-
Master in Ecological Management of Catchments in Europe (www.ecocatch.eu)
-
Master in Marine Sciences- Marine Resources, Univ. Porto
-
Master in Environmental Contamination and Toxicology, Univ. Porto
-
Master in Aquaculture and Fisheries, Univ. Algarve
-
Master in Marine Biology, Univ. Algarve
-
Master in Biomedical Sciences, Univ. Algarve
Organization of advanced training courses, workshops and internal seminars series.
-
MarBEF Workshop on a European Monitoring Network, Spring School for an European
Monitoring Network, 13-16 March, Porto.
-
XVI Jornadas de Classificação e Análise de Dados (JOCLAD), 2-4 April, Faro.
-
Workshop Douro and Minho - Estuaries and Costal Zone, 16-17 April, Porto.
-
April, Tavira.
-
Course Introduction to Statistics using the R, 15-18 June, Lisbon.
-
4th MedCLIVAR Workshop, 28-30 Sept, Sesimbra.
-
Workshop on Aquatic Organisms vs Animal Experimentation, 7 October, Porto.
-
Course on 2D-DIGE, 13-15 October, Faro.
-
ESF Magellan Workshop: The Shackleton Sites, 9-10 November, Lisbon.
-
Workshop: Plano de Ordenamento do Estuário do Rio Douro, 23 November, Porto.
-
Internal
seminars
in
Faro
and
Porto
(http://www.cimar.org/cimar2008/conferences.htm)
on
a
fortnight
basis.
9d. Outreach/science and society
1. Outreach/science and society (Activities that the LA as a whole)
CIMAR has performed a significant effort in the promotion of outreach/Science and Society
activities, to foster the dialogue between scientists and society, to improve the public
311
knowledge and perception of science and to increase the interest of the young students towards
scientific careers. Some of the most relevant activities include:
Scientific management of public equipment
-
CMIAs - Environmental Monitoring and Interpretation Centres
CIMAR is directly managing CMIA-Vila do Conde, through a protocol with the
municipality (www.cmia-viladoconde.net). The centre has displayed several temporary
exhibitions, organized conferences and professional courses and performed regular in
house and field activities for public of different ages, with a particular emphasis on
young students. 3400 visitor in 2009, 52 schools/institutions (including from Spain).
-
Public aquariums and fisheries museums
Aguda Littoral Station - ELA (www.fundacao-ela.pt, ca 50000 visitors/year) and River
Minho AQUAMuseum (www.cimsoft.pt/aquamuseu, ca 30000 visitors/year) have been
managed by CIMAR researchers. Both stations have a special relevance in what
concerns Environmental Education Programs for all age.
Participation in the Ciência Viva Programme (http://www.cienciaviva.pt)
-
-
312
Several types of activities have been implemented at both CCMAR and CIIMAR, to
contribute to the promotion of a scientific and technological culture among the
Portuguese population and more specifically to encourage the use of experimental
methods in science learning, involving both scientific and school communities.
-
Scientists go to School: presentation of scientific lectures at elementary and
high schools and promotion of a summer course related with marine
biodiversity, in collaboration with the Algarve Ciência Viva Centres.
-
Science on Holidays for Young People: preparation of scientific activities (1-2
weeks each) aiming to offer high school students the opportunity for first-hand
contact with the reality of scientific research work during their summer
vacations.
-
Biology Activities in the Summer: preparation of field activities (4-8 hours each)
for general public of all ages, in particular family groups.
-
Exploring the Ocean at School: collaboration with the Ciência Viva Centres of
Vila do Conde and Algarve in the implementation of the Ocean Exploration in
the International Education Community programme (Explore) of NOAA, USA.
-
Participation in the Science and Technology Week, 21-27 November.
-
Participation in other events such as “Encontros Imediatos com a Ciência”, and
“Café da Ciência”.
Sea Itinerant University (UIM) (http://www.cimar.org/CIIMAR/en/hz_pas_uim.htm)
UIM is a program that aims to promote the maritime identity of Portuguese and Spanish
students, through the contact with the sea and its potentialities, related activities and
culture. This initiative had its first edition in 2006, and it is organized by the Univ of
Porto, trough CIMAR, and the Univ of Oviedo. Courses are organised in land and on
board the vessel CREOULA, a 4-masted training ship of the Portuguese Navy built in
1937. The theme of the 2009 course was "Atlantic, an open frontier".
-
MOBIDIC - School in the Beach (http://www.estgm.ipb.pt/~projig/mobidic)
MoBIDiC is an outreach program related with monitoring of intertidal biodiversity in
beaches of the North coast of Portugal. This initiative had its first edition in 2005, and at
present involves 12 high schools and 400 students.
-
Visionarium - Europarque Science Centre
CIMAR in collaboration with the Visionarium - Europarque Science Centre, is involved in
a national project using Daphnia as biological model for experimental teaching of
sciences in high schools. 220 schools, 320 teachers, 2500 students (2008-2009).
(http://projectodaphnia0.blogs.sapo.pt/).
-
-
Participation in other events and exhibitions
-
LAB-IT®-The Itinerant Laboratory in Molecular Genetis, project aiming to bring
molecular biology and genetics to high schools.
-
Junior University (http://universidadejunior.up.pt/), within the School of Life
and Health Sciences for high school students, 30 Aug-4 Sept.
-
“Science, Education and Innovation” exhibition by the Univ of Porto, through
the presentation of the research developed at CIMAR to encourage high school
students to engage in scientific careers, 26-29 March.
-
EXPOMAR exhibition, 29 April-3 May.
CIMAR in the media
CIMAR activities have been also disseminated through the media: publications in nonscientific journals and newspapers (e.g. CORDIS News, National Geographic, DN,
Expresso, Agência Lusa, Visão, Ciência Hoje), participation in TV and radio programs (i.e.
Rádio Renascença, Antena 1, SIC, RDP África).
313
9e. International events
1. Organization of international events (Events that are international in dimension and
required the involvement of the LA for their implementation)
In 2009, CIMAR organized several international scientific conferences and workshops. Most
international relevant events included:
-
European Conference on Marine Natural Products, 19-23 July, Porto, Portugal.
http://www.cimar.org/6ECMNP/
-
1st
Iberian
Congress
in
http://www.cimar.org/cic2009/
-
7th AIEC Congress, Iberian Association of Comparative Endocrinology, 6-9 September,
Porto, Portugal. http://www.ciimar.up.pt/7aiec/index.html
-
-
April, Tavira, Portugal.
-
Session ET03 - Assessing the ecological relevance of OMICS and biomarkers, SETAC
Europe 19th Annual Meeting, 31 May-4 June, Goteborg, Sweden.
-
4th MedCLIVAR Workshop, 28-30 September, Sesimbra, Portugal.
-
MarBEF Workshop on a European Monitoring Network 2009, 13-14 March, Porto,
Portugal.
-
European Science Foundation Magellan Workshop: The Shackleton Sites, 9-10
November, Lisbon, Portugal.
-
Faro Ocean Tracking Network (OTN), 3-5 December, Faro, Portugal. Number of
participants: 65.
-
Workshop: Douro and Minho - Estuaries and Costal Zone, 16-17 April, Porto, Portugal.
-
Workshop: Plano de Ordenamento do Estuário do Rio Douro, 23 November, Porto,
Portugal.
Cyanotoxins,
6-7
July,
Porto,
Portugal.
Participating as members of Scientific Committees (e.g.):
-
2nd European Congress for Conservation Biology, 2-7 September, Prague, Czeck
Republic.
-
43rd European Marine Biology Symposium, 8-12 September, Ponta Delgada, Portugal.
314
-
X Iberian Meeting of Toxic Phytoplankton and Biotoxins, IPIMAR, 12-15 May, Lisbon,
Portugal.
Internal Seminars Series at CIIMAR and CCMAR, all year.
Events programmed for 2010:
-
practice, 29 January 2010, Faro, Portugal.
-
Workshop New Tools for better planning, response management and damage
assessment in HNS, inert and oil spills, 30 April 2010, European Agency for Maritime
Safety (EMSA), Lisbon, Portugal.
-
International Symposium FloraMac2010, 23-25 September 2010, Ponta Delgada,
Portugal.
315
10. INTERNAL EVALUATIONS
10.1 Summary of internal evaluations during 2008
During 2009, the contact with members of the External Advisory Board was mantain, namely
with Prof. Steve Hawkins for advising on promoting research capability in the general area of
marine biodiversity and ecosystems science. The internal evaluation of the External Advisory
Board was transfer for the 1st semester of 2010, simultaneously with the annual meeting of
CIMAR-LA, as we were expecting the results of the external evaluation carried out by FCT in
2008 during the 2nd semester of 2009, and not yet deliver.
10.2 Future internal evaluations programmed for 2009
Since the results of the external evaluation carried out by FCT were not delivered during the 2nd
semester of 2009, the Direction of CIMAR LA decided to transfer the internal evaluation of the
External Advisory Board for the 1st semester of 2010.
Important modifications in the organization of the centre need to be discussed and
implemented without delay, in order to avoid any detrimental effects in the good performance
of the centre. These alterations mainly concern the admission of a significant nr. of researchers
in physical oceanography and coastal dynamics and engineering, adding interdisciplinary to the
centre and suggesting the creation of another research line specific for oceanic and coastal
dynamics. As such, the evaluation of the External Advisory Board will be conducted in the 1st
semester of 2010, independently of the release of the results of the external evaluation carried
out by FCT.
316
11. FUTURE OBJECTIVES
11.1 Summary of proposed future objectives
Future (5 year) objectives of CIMAR LA reflect the multidisciplinary nature of Marine Sciences
and are aimed at consolidating existing lines of research, synergistic interactions of research
teams, and integrating new approaches that may contribute to a holistic view of marine
ecosystems, responsiveness to changes of natural or anthropogenic origin and contribution to
social, educational and economic sectors.
Specific objectives are:
-
Develop and contribute to integrate marine biodiversity initiatives and databases at a
national and European level through research networks.
-
Develop genomics and post-genomics capabilities to understand biogeochemical
processes, short and long-term adaptation including species invasions, extreme
environments, analysis of historical samples and potential biotechnological applications
such as marine biofuels.
-
Develop physical oceanography and modelling competences which can be associated
with existing remote sensing and biology expertise to understand ecosystem dynamics,
dispersion of pollutants and long term climatic variations.
-
Establish risk management procedures for natural toxins and xenobiotics by integrating
information from multiples levels of biological organization from molecules to
ecosystem.
-
Integrate social sciences in the evaluation of ecosystem services including fisheries.
-
Develop analytical chemistry and bioscience platforms (“omics”, imaging) to support the
LA and provide external services.
-
Stimulate technology transfer, setting up of spin offs, startups and partnerships with
industry, in particular aquaculture.
-
Improve and stimulate cooperation between different research groups within research
lines and across research lines with the aim of reducing fragmentation and stimulating
cooperation.
-
To further increase the national and foster the international attractiveness of the PhD
and MSc programmes in which the CIMAR LA is an institutional partner.
-
To promote public understanding of sciences for the general public educational
programmes targeting schools at all levels.
-
Strive to improve cost-effectiveness of research and reduce waste and carbon footprint.
317
SCIENTIFIC OUTPUT 2009
A.
PUBLICATIONS
EDITED BOOKS
1.
Cabral, JP. 2009. Gonçalo Sampaio. Professor e Botânico Notável. Editora da Universidade do
Porto, Série Memória 1, Porto, 338 p. ISBN 978-972-8025-84-7.
2.
Cabral, JP. 2009. Gonçalo Sampaio. Vida e Obra – Pensamento e Acção. Edição da Câmara
Municipal da Póvoa de Lanhoso, Póvoa de Lanhoso, 301 p. ISBN 972-96776-5-8.
3.
Chicharo, L, Wagner, I, Chicharo, M, Lapsinka, M, Zalewski, M. 2009. Practical experiments guide
for Ecohydrology. UNESCO Manual, Faro, Portugal, 121 p. ISBN 978-989-20-1702-0.
4.
Heip, C, Hummel, H, van Avesaath, P, Appeltans, W, Arvanitidis, C, Aspden, R, Austen, M, Boero,
F, Bouma, TJ, Boxshall, G, Buchholz, F, Crowe, T, Delaney, A, Deprez, T, Emblow, C, Feral, JP,
Gasol, JM, Gooday, A, Harder, J, Ianora, A, Kraberg, A, Mackenzie, B, Ojaveer, H, Paterson, D,
Rumohr, H, Schiedek, D, Sokolowski, A, Somerfield, P, Sousa Pinto, I, Vincx, M, Węsławski, JM,
Nash, R. 2009. Marine Biodiversity and Ecosystem Functioning. Printbase, Dublin, Ireland. ISSN
2009-2539.
5.
Neto, AI, Wallenstein, FM, Álvaro, NV, Azevedo, JMN. 2009. Guias Costeiros dos Açores: Ilha
Graciosa [Coastal Guides of the Azores: Graciosa Island]. Secretaria Regional do Ambiente e do
Mar e Câmara Municipal de Santa Cruz, Graciosa, Açores, Portugal.
6.
Ozório, ROA. 2009. L-Carnitine and Energy Metabolism in Aquaculture. Growth Response of
Catfish to Dietary L-Carnitine. LAP Lambert Academic Publishing AG & Co. KG. ISBN 978-3-83830560-8.
7.
Wallenstein, FM, Neto, AI, Álvaro, NV, Tittley, I, Azevedo, JMN. 2009. Metodologias para
caracterização de biótopos e caracterização geral de comunidades costeiras em Ilhas Oceânicas
[Methodologies for Biotope Surveys and General Benthic Coastal Community Characterization in
Oceanic Islands]. Secretaria Regional do Ambiente e do Mar, Portugal.
BOOKS (Chapters)
1.
Afonso, MJ, Chaminé, HI, Gomes, A, Fonseca, PE, Marques, JM, Guimarães, L, Guilhermino, L,
Teixeira, J, Carvalho, JM, Rocha, FT. 2009. Urban hydrogeomorphology and geology of the Porto
metropolitan area (NW Portugal): a multidisciplinary approach. In: Culshaw, MG, Reeves, HJ,
Jefferson, I, Spink, TW (Eds). Engineering Geology for Tomorrow's Cities. Engineering Geology
Special Publication, The Geological Society of London, Volume 22 (on CD-Rom insert, IAEG Paper
92, 9 p.). ISBN 978-1-86239-290-298.
2.
Almeida, CMR, Mucha, AP, Carvalho, PN, Basto, MCP, Vasconcelos, MTSD. 2009. Mutual
endpoints and rhizoremediation. In: Ramsey, G, McHugh, S (Eds). River Sediments. Frank
Columbus Ed., Nova Science Publishers. ISBN 978-1-60741-437-7.
3.
Barata, EN, Gonçalves, DM. 2009. Communication in Blennies. In: Patzner, RA, Gonçalves, EJ,
Hastings, PA, Kapoor, BG (Eds). The Biology of Blennies, Enfield, Science Publishers Inc., USA, pp.
351-378. ISBN 978-1-57808-439-5.
4.
Chicharo, L. 2009. May bivalves be used to control toxic algae blooms?. In: Chicharo, L, Wagner, I,
Chicharo, M, Lapsinka, M, Zalewski, M (Eds). Practical Experiments Guide for Ecohydrology.
UNESCO Manual, Faro, Portugal, pp. 55-58. ISBN 978-989-20-1702-0.
5.
Chicharo, MA. 2009. Are males specimens more adequate to detect antropogenic impacts? In:
Chicharo, L, Wagner, I, Chicharo, M, Lapsinka, M, Zalewski, M (Eds). Practical Experiments Guide
for Ecohydrology. UNESCO Manual, Faro, Portugal, pp. 79-100. ISBN 978-989-20-1702-0.
6.
Cidade, H, Neves, M, Kijjoa, A. 2009. Natural prenylated flavones: chemistry and biological
activies - An overview. In: Brahmachari, G (Ed). Natural Products: Chemistry, Biochemistry and
Pharmacology. Narosa Publishing House Pvt. Ltd., New Delhi, India, pp. 463-519. ISBN: 9781842654507.
7.
Dias, S, Sousa, R, Lobón-Cerviá, J, Laffaille, P. 2009. The decline of diadromous fish in Western
(Eds). Fisheries: Management, Economics and Perspectives. Nova Science Publishers Inc., New
York, USA, pp. 67-92. ISBN: 978-1-60692-303-0.
8.
Kijjoa, A, Vieira, LMM. 2009. Triterpenes from the plants of the Family Clusiaceae (Guttiferae):
Chemistry and biological activities. In: Brahmachari, G (Ed). Natural Products: Chemistry,
Biochemistry and Pharmacology. Narosa Publishing House Pvt. Ltd., New Delhi, India, pp. 326381. ISBN: 978-1842654507.
9.
Lino, PG, Bentes, L, Abecasis, D, Santos, MN, Erzini, K. 2009. Comparative behavior of wild and
hatchery reared white seabream (Diplodus sargus) released on artificial reefs off the Algarve
(southern Portugal). In: Nielsen, JL, Arrizabalaga, H, Fragoso, N, Hobday, A, Lutcavage, M, Sibert, J
(Eds). Tagging and Tracking of Marine Animals with Electronic Devices - Reviews: Methods and
Technologies in Fish Biology and Fisheries, vol. 9, pp. 23-34. ISSN 1571-3075.
10. McQuaid, C, Arenas, F. 2009. Biological Invasions: insights from marine benthic communities. In:
Wahl, M (Ed). Marine Hard Bottom Communities: Patterns, Scales, Dynamics, Functions, Shifts.
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11. Moraes, JRE, Iwashita, MKP, Ozório, ROA, Rema, P, Moraes, FR. 2009. Influence of vitamin E
supplementation on dermal wound healing in Tilapia, Oreochromis niloticus. In: De Carlo, F,
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12. Power, DM, Morgado, I, Cardoso, JCR. 2009. Evolutionary Insights from fish transthyretin. In:
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PUBLICATIONS IN INTERNATIONAL PEER REVIEWED JOURNALS
1.
Abecasis, D, Bentes, L, Erzini, K. 2009. Home range, residency and movements of Diplodus sargus
and Diplodus vulgaris in a coastal lagoon: connectivity between nursery and adult habitats.
Estuarine, Coastal and Shelf Science 85: 525-529. IF=1.970.
2.
Abecasis, D, Cardigos, F, Almada, F, Gonçalves, JMS. 2009. New records on the ichthyofauna of
the Gorringe seamount (Northeastern Atlantic). Marine Biology Research 5: 605-611. IF=1.000.
3.
Abrantes, F, Lopes, C, Rodrigues, T, Gil, I, Witt, L, Grimalt, J, Harris, I. 2009. Proxy calibration to
instrumental data set: Implications for paleoceanographic reconstructions. Geochemistry,
4.
Abreu, MH, Varela, DA, Henríquez, L, Villarroel, A, Yarish, C, Sousa-Pinto, I, Buschmann, AH. 2009.
Traditional vs integrated multi-trophic aquaculture of Gracilaria chilensis. CJ Bird, J McLachlan &
EC Oliveira: productivity and physiological performance. Aquaculture 293: 211-220. IF=1.925.
5.
Agüero-Chapin, G, Varona, J, de la Riva, GA, Antunes, A, González-Villa, T, Uriarte, E, GonzálezDíaz, H. 2009. Alignment-free prediction of polygalacturonases with pseudo-folding topological
indices: experimental isolation from Coffea Arabica and prediction of a new sequence. Journal of
Proteome Research 8(4): 2122-2128. IF=5.132.
6.
Alberto, F, Whitmer, A, Coelho, A, Zippay, M, Varela-Alvarez, E, Raimondi, PT, Reed, D, Serrão,
EA. 2009. Microsatellite markers for the giant kelp Macrocystis pyrifera. Conservation Genetics
10: 1915-1917. IF=1.849.
7.
Alberto, F. 2009. MsatAllele_1.0: an R package to visualize the binning of microsatellite alleles.
Journal of Heredity 100(3): 394-397. IF=2.052.
8.
Almany, GR, De Arruda, MP, Arthofer, W, Atallah, ZK, Beissinger, SR, Berumen, ML, Bogdanowicz,
SM, Brown, SD, Bruford, MW, Burdine, C, Busch, JW, Campbell, NR, Carey, D, Carstens, BC, Chu,
KH, Cubeta, MA, Cuda, JP, Cui, ZX, Datnoff, LE, Davila, JA, Davis, ES, Davis, RM, Diekmann, OE,
Eizirik, E, Fargallo, JA, Fernandes, F, Fukuda, H, Gale, LR, Gallagher, E, Gao, YQ, Girard, P, Godhe,
A, Goncalves, EC, Gouveia, L, Grajczyk, AM, Grose, MJ, Gu, ZF, Hallden, C, Harnstrom, K,
Hemmingsen, AH, Holmes, G, Huang, CH, Huang, CC, Hudman, SP, Jones, GP, Kanetis, L,
Karunasagar, I, Karunasagar, I, Keyghobadi, N, Klosterman, SJ, Klug, PE, Koch, J, Koopman, MM,
Koppler, K, Koshimizu, E, Krumbock, S, Kubisiak, T, Landis, JB, Lasta, ML, Lee, CY, Li, QQ, Li, SH,
Lin, RC, Liu, M, Liu, N, Liu, WC, Liu, Y, Loiseau, A, Luan, WS, Maruthachalam, KK, McCormick, HM,
Mellick, R, Monnahan, PJ, Morielle-Versute, E, Murray, TE, Narum, SR, Neufeld, K, de Nova, PJG,
Ojiambo, PS, Okamoto, N, Othman, AS, Overholt, WA, Pardini, R, Paterson, IG, Patty, OA, Paxton,
RJ, Planes, S, Porter, C, Pratchett, MS, Puttker, T, Rasic, G, Rasool, B, Rey, O, Riegler, M, Riehl, C,
Roberts, JMK, Roberts, PD, Rochel, E, Roe, KJ, Rossetto, M, Ruzzante, DE, Sakamoto, T,
Saravanan, V, Sarturi, CR, Schmidt, A, Schneider, MPC, Schuler, H, Serb, JM, Serrao ETA, Shi, YH,
Silva, A, Sin, YW, Sommer, S, Stauffer, C, Strussmann, CA, Subbarao, KV, Syms, C, Tan, F, Tejedor,
ED, Thorrold, SR, Trigiano, RN, Trucco, MI, Tsuchiya-Jerep, MTN, Vergara, P, van de Vliet, MS,
Wadl, PA, Wang, AM, Wang, HX, Wang, RX, Wang, XW, Wang, Y, Weeks, AR, Wei, FW, Werner,
WJ, Wiley, EO, Williams, DA, Wilkins, RJ, Wisely, SM, With, KA, Wu, DH, Yao, CT, Yau, C, Yeap, BK,
Zhai, BP, Zhan, XJ, Zhang, GY, Zhang, SY, Zhao, R, Zhu, LF. Permanent genetic resources added to
molecular ecology resources database 1 May 2009-31 July 2009. Molecular Ecology Resources
9(6): 1460-1466. IF=1.251.
9.
Almeida, AM, Campos, A, van Harten, S, Cardoso, LA, Varela Coelho, A. 2009. Establishment of a
proteomic reference map for the gastrocnemius muscle in the rabbit (Oryctolagus cuniculus).
Research in Veterinary Science 87: 196-199. IF=1.345.
10. Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, AA, Vasconcelos, MTSD. 2009. Study of the
Estuarine, Coastal and Shelf Science 85: 627-632. IF=1.970.
11. Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, AA, Vasconcelos, MTSD. 2009. Influence of
12. Almeida, R, Gomez-Zavaglia, A, Kaczor, A, Ismael, A, Cristiano, MLS, Fausto, R. 2009. Matrixisolation FTIR, theoretical structural analysis and reactivity of amino-saccharins: N-(1,1-dioxo-1,2benzisothiazol-3-yl)-N-methyl amine and N,N-dimethyl amine. Journal of Molecular Structure
938(1-3): 198-206. IF=1.551.
13. Amat, N, Upur, H, Ablimit, A, Matsidik, A, Yusup, A, Kijjoa, A. 2009. Immunomodulatory effects of
abnormal Savda Munsiq, a traditional Uighur medicine, on the combined stress mice. Journal of
Ethnopharmacology 122: 42-47. IF=2.322.
14. Amorim, A, Veloso, V, Frazão, B, Alves de Matos, AP. 2009. TEM identification of Pseudo-nitzschia
species from Lisbon Bay (NE Atlantic). Microscopy and Microanalysis 15(S3): 9-10. IF=3.035.
15. Araujo, NCP, Barton, V, Jones, M, Stocks, PA, Ward, SA, Davies, J, Bray, PG, Shone, AE, Cristiano,
MLS. 2009. Semi-synthetic and synthetic 1,2,4-trioxaquines and 1,2,4-trioxolaquines: synthesis,
preliminary SAR and comparison with acridine endoperoxide conjugates. Biorganic & Medicinal
Chemistry Letters 19(7): 2038-2043. IF=2.650.
16. Araújo, R, Bárbara, I, Tibaldo, M, Berecibar, E, Díaz Tapia, P, Pereira, R, Santos, R, Sousa Pinto, I.
2009. Checklist of benthic marine algae of Northern Portugal. Botanica Marina 52: 24-46.
IF=1.090.
18. Arenas, F, Rey, F, Sousa-Pinto, I. 2009. Diversity effects beyond species richness: evidence from
intertidal macroalgal assemblages. Marine Ecology Progress Series 381: 99-108. IF=2.519.
19. Arnaud-Haond, S, Teixeira, S, Terrados, J, Tri, NH, Hong, NP, Duarte, CM, Serrao, EA. 2009.
Genetic diversity of mangrove Avicennia alba three decades after Agent Orange. Marine Ecology
20. Ascenso, RMT, Leite, RB, Afonso, R, Cancela, ML. 2009. Expression pattern of Perkinsus olseni
genes in response to bivalves with different susceptibility to Perkinsosis. Journal of Fish Diseases
32(7): 633-636. IF=1.697.
21. Assis, J, Tavares, D, Tavares, JT, Cunha, AH, Alberto, FA, Serrão, EA. 2009. Findkelp, a GIS-based
community participation project to assess Portuguese kelp conservation status. Journal of
Coastal Research SI 56: 1469-1473. IF=1.366.
22. Auamcharoen, W, Chandrapatya, A, Kijjoa, A, Silva, Herz, W. 2009. Chemical constituents of
Duabanga grandiflora (Lythraceae). Biochemical Systematics and Ecology 37: 535-537. IF=1.131.
23. Auamcharoen, W, Kijjoa, A, Chandrapatya, A, Pinto, MM, Silva, AMS, Naengchomnong, W, Herz,
W. 2009. A new tetralone from Diospyros cauliflora. Biochemical Systematics and Ecology 37:
690-692. IF=1.131.
24. Aureliano, M, Crans, DC. 2009. Decavanadate (V10O286-) and oxovanadates: Oxometalates with
many biological activities. Journal of Inorganic Biochemistry 103(4): 536-546. IF= 3.252.
25. Aureliano, M. 2009. Decavanadate: a journey in a search of a role. Dalton Transactions 42: 90939100. IF=4.081.
26. Azevedo, C, Casal, G, Garcia, P, Matos, P, Teles-Grilo, L, Matos, E. 2009. Ultrastructural and
phylogenetic data of Chloromyxum riorajum sp. nov. (Myxozoa), a parasite of the stingray Rioraja
agassizii in Southern Brazil. Diseases of Aquatic Organisms 85: 41-51. IF=1.687.
27. Azevedo, C, Casal, G, Matos, P, Ferreira, I, Matos, E. 2009. Light and electron microscopy of the
spore of Myxobolus heckelii n. sp. (Myxozoa), parasite from the Brazilian fish Centromochlus
heckelii (Teleostei, Auchenipteridae). Journal of Eukaryotic Microbiology 56: 589-593. IF=2.355.
28. Azevedo, C, Casal, C, Mendonça, I, Matos, E. 2009. Fine structure of Henneguya sp. n. (Myxozoa),
a parasite of the gills of the Brazilian teleostean fish Hemiodopsis microlepes (Hemiodontidae).
Memórias do Instituto Oswaldo Cruz 104(7): 975-979. IF=2.097.
29. Baker, DW, Morgan, JD, Wilson, JM, Matey, V, Hueng, K, Brauner, CJ. 2009. Complete
intracellular pH protection during extracellular pH depression is associated with hypercarbia
tolerance in white sturgeon, Acipenser transmontanus. American Journal of Physiology 296:
R1868-R1880. IF=3.661.
IF=3.124.
31. Barata, EN, Hubert, F, Conceicao, LEC, Velez, Z, Rema, P, Hubbard, PC, Canario, AVM. 2009. Prey
odour enhances swimming activity and feed intake in the Senegalese sole. Aquaculture 293: 100107. IF=1.925.
32. Barrento, S, Marques, A, Teixeira, B, Anacleto, P, Vaz-Pires, P, Carvalho, ML, Nunes, ML. 2009.
Macro and trace elements in two populations of brown crab Cancer pagurus: Ecological and
human health implications. Journal of Food Composition and Analysis 22(1): 65-71. IF=2.423.
33. Barrento, S, Marques, A, Teixeira, B, Anacleto, P, Vaz-Pires, P, Nunes, ML. 2009. Effects of season
on the chemical composition and nutritional quality of the edible crab Cancer pagurus. Journal of
Agricultural and Food Chemistry 57(22): 10814-10824. IF=2.469.
34. Barrento, S, Marques, A, Teixeira, B, Carvalho, ML, Vaz-Pires, P, Nunes, ML. 2009. Accumulation
of elements (S, As, Br, Sr, Cd, Hg, Pb) in two populations of Cancer pagurus: Ecological
implications to human consumption. Food and Chemical Toxicology 47: 150-156. IF=2.114.
35. Barrento, S, Marques, A, Teixeira, B, Carvalho, ML, Vaz-Pires, P, Nunes, ML. 2009. Influence of
season and sex on the contents of minerals and trace elements in brown crab (Carcinus pagurus,
Linnaeus, 1758). Journal of Agricultural and Food Chemistry 57(8): 3253-3260. IF=2.469.
36. Barrento, S, Marques, A, Teixeira, B, Vaz-Pires, P, Nunes, ML. 2009. Nutritional quality of the
edible tissues of european lobster Homarus gammarus and american lobster Homarus
americanus. Journal of Agricultural and Food Chemistry 57(9): 3645-36552. IF=2.469.
37. Barros, RJ, Jesus, C, Martins, M, Costa, MC. 2009. Marble stone processing powder residue as
chemical adjuvant for the biologic treatment of acid mine. Process Biochemistry 44: 477-480.
IF=2.444.
38. Bartels-Jonsdottir, HB, Voelker, AHL, Knudsen, KL, Abrantes, F. 2009. Twentieth century warming
and hydrographical changes in the Tagus Prodelta, Eastern North Atlantic. Holocene 19: 369-380.
IF=2.481.
39. Batista-Pinto, C, Rocha, E, Castro, LFC, Lobo-da-Cunha, A. 2009. Seasonal and gender variation of
peroxisome proliferator activated receptors expression in brown trout liver. General and
Comparative Endocrinology 161: 146-152. IF=2.732.
40. Bebianno, MJ, Barreira, LA. 2009. Polycyclic aromatic hydrocarbons concentrations and
biomarker responses in the clam Ruditapes decussatus transplanted in the Ria Formosa lagoon.
41. Beirão, J, Soares, F, Herraez, MP, Dinis, MT, Cabrita, E. 2009. Sperm quality evaluation in Solea
senegalensis during the reproductive season at cellular level. Theriogenology 72: 1251-1261.
IF=2.073.
42. Berecibar, E, Wynne, MJ, Bárbara, I, Santos, R. 2009. New records of Rhodophyta to the flora of
the coast of the Iberian Peninsula. Botanica Marina 52: 217-228. IF=1.090.
43. Berecibar, E, Wynne, MJ, Cremades, J, Santos, R. 2009. Report of the brown alga Zosterocarpus
oedogonium (Ectocarpales) from Portugal, its first recorded occurrence outside of the
Mediterranean Sea. Nova Hedwigia 89: 237-244. IF=0.763.
44. Berecibar, E, Wynne, MJ, Santos, R. 2009. Contarinia squamariae (Rhizophyllidaceae,
Rhodophyta): first report from Portugal. Botanica Marina 52: 15-23. IF=1.090.
45. Berecibar, E, Wynne, MJ, Santos, R. 2009. Report of the red alga Gulsonia nodulosa (Ceramiales)
from Portugal, its first recorded occurrence outside of the Mediterranean Sea. Nova Hedvigia 88:
23-31. IF=0.763.
46. Bird, G, Larsen, K. 2009. Tanaidacean Phylogeny: The second step. The basal Paratanaoidean
families (Crustacea: Malacostraca). Arthropod Systematics & Phylogeny 67(2): 137-158.
47. Borges, P, Oliveira, B, Casal, S, Dias, J, Conceicão, L, Valente, LMP. 2009. Dietary lipid level affects
growth performance and nutrient utilisation of Senegalese sole (Solea senegalensis) juveniles.
British Journal of Nutrition 102: 1007-1014. IF=3.446.
48. Borges, R, Vaz, J, Serrão, EA, Gonçalves, EJ. 2009. Short-term temporal fluctuation of verynearshore larval fish assemblages at the Arrábida Marine Park. Journal of Coastal Research SI 56:
376-380. IF=1.366.
49. Burbidge, C, Dias, M, Prudêncio, M, Rebelo, L, Cardoso, G, Brito, P. 2009. Internal alpha activity:
localization, compositional associations and effects on OSL signals in quartz approaching beta
saturation. Radiation Measurements 44: 494-500. IF=0.973. (*)
50. Cabaço, S, Machás, R, Santos, R. 2009. Individual and population plasticity of the seagrass Zostera
noltii along a vertical intertidal gradient. Estuarine, Coastal and Shelf Science 82: 301-308.
IF=1.970.
51. Cabrita, E, Engrola, S, Conceicão, LEC, Pousão-Ferreira, P, Dinis, MT. 2009. Successful
cryopreservation of sperm from sex-reversed dusky grouper, Epinephelus marginatus.
Aquaculture 287: 152-157. IF=1.925.
52. Cahu, CL, Gisbert, E, Villeneuve, LAN, Morais, S, Hamza, N, Wold, PA, Infante, JLZ. 2009. Influence
os dietary phospholipids on early ontogenesis of fish. Aquaculture Research 40(9): 989-999.
IF=1.099.
53. Cairrão, E, Pereira, MJ, Morgado, F, Nogueira, AJA, Guilhermino, L, Soares, AMVM. 2009.
Phenotypic variation of Fucus ceranoides, F. spiralis and F. vesiculosus in a temperate coast (NW
Portugal). Botanical Studies 50: 205-215. IF=0.781.
54. Calado, R, Vitorino, A, Reis, A, Lopes da Silva, T, Dinis, MT. 2009. Effect of different diets on larval
production, quality and fatty acid profile of the marine ornamental shrimp Lysmata amboinensis
(de Man, 1888), using wild larvae as a standard. Aquaculture Nutrition 15: 484-491. IF=1.482.
55. Campos, A, da Costa, G, Coelho, VA, Fevereiro. P. 2009. Identification of bacterial protein
markers and enolase as a plant response protein in the infection of Olea europaea subsp.
europaea by Pseudomonas savastanoi pv. savastanoi. European Journal of Plant Pathology 125:
603-616. IF=1.931.
56. Campos, J, Freitas, V, Pedrosa, C, Guillot, R, Van der Veer, HW. 2009. Latitudinal variation in
growth of Crangon crangon (L.): does counter-gradient growth compensation occur?. Journal of
Sea Research 62: 229-237. IF=1.803.
57. Campos, J, Pedrosa, C, Rodrigues, J, Santos, S, Witte, J, Santos, P, Veer, H. 2009. Population
zoogeography of brown shrimp Crangon crangon along its distributional range based on
morphometric characters. Journal of the Marine Biological Association of the United Kingdom
89(3): 499-507. IF=0.995.
58. Campos, J, van der Veer, HW, Freitas, V, Kooijman, SALM. 2009. Contribution of different
generations of the brown shrimp Crangon crangon (L.) in the Dutch Wadden Sea to commercial
fisheries: A dynamic energy budget approach. Journal of Sea Research 62: 106-113. IF=1.803.
different conditions of water calcium availability in Tetraodon nigroviridis. The Journal of
Physiological Sciences 59(S1): 536. IF=1.125.
60. Canning-Clode, J, Bellou, N, Kaufmann, MJ, Wahl, M. 2009. Local-regional richness relationship in
fouling assemblages - effects of succession. Basic and Applied Ecology 10: 745-753. IF=2.422.
system. The Journal of Physiological Sciences 59(S1): 98. IF=1.125.
62. Cardoso, JFMF, Witte, JI, van der Veer, HW. 2009. Reproductive investment of the American
razor clam Ensis americanus in the Dutch Wadden Sea. Journal of Sea Research 62: 295-298.
IF=1.803.
63. Cardoso, JFMF, Witte, JIJ, van der Veer, HW. 2009. Differential reproductive strategies of two
bivalves in the Dutch Wadden Sea. Estuarine, Coastal and Shelf Science 84: 37-44. IF=1.970.
64. Carrola, J, Fontaínhas-Fernandes, A, Matos, P, Rocha, E. 2009. Liver histopathology in brown
trout (Salmo trutta f. fario) from the Tinhela River, subjected to mine drainage from the
abandoned Jales mine (Portugal). Bulletin of Environmental Contamination and Toxicology 83:
35-41. IF=0.992.
65. Carvalho, PN, Rodrigues, PNR, Basto, MCP, Vasconcelos, MTSD. 2009. Organochlorine pesticides
levels in Portuguese coastal areas. Chemosphere 75: 595-600. IF=3.253.
66. Carvalho, PN, Rodrigues, PNR, Basto, MCP, Vasconcelos, MTSD. 2009. Butyltin levels in several
Portuguese coastal areas. Environmental Monitoring and Assessment 159: 183-190. IF=1.356.
67. Carvalho, S, Falcão, M, Cúrdia, J, Moura, A, Serpa, D, Gaspar, MB, Dinis, MT, Pousão-Ferreira, P,
Cancela da Fonseca, L. 2009. Benthic dynamics within a land-based semi-intensive aquaculture
fish farm: the importance of settlement ponds. Aquaculture International 17: 571-587. IF=0.753.
68. Casal, G, Garcia, P, Matos, P, Monteiro, E, Matos, E, Azevedo, C. 2009. Fine structure of
Chloromyxum menticirrhi n. sp. (Myxozoa) infecting the urinary bladder of the marine teleost
Menticirrhus americanus (Sciaenidae) in Southern Brazil. European Journal of Protistology 45:
139-146. IF=1.966.
69. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2009. Morphological and genetical description of
Loma psittaca sp. n. isolated from the Amazonian fish Colomesus psittacus. Parasitology
Research 105: 1261-1271. IF=1.721.
70. Castanheiro, RAP, Pinto MM, Cravo, SMM, Pinto, DCGA, Silva, AMS, Kijjoa, A. 2009. Improved
methodologies for synthesis of prenylated xanthones by microwave irradiation and combination
of heterogeneous catalysis (K10 clay) with microwave irradiation. Tetrahedron 65: 3848-3857.
IF=3.219.
71. Castro, LFC, Rocha, MJ, Lobo-da-Cunha, A, Batista-Pinto, C, Machado, A, Rocha, E. 2009. The 17βhydroxysteroid dehydrogenase 4: gender-specific and seasonal gene expression in the liver of
brown trout (Salmo trutta f. fario). Comparative Biochemistry and Physiology B - Biochemistry &
Molecular Biology 153: 157-164. IF=1.607.
72. Cavaleiro, F, Santos, MJ. 2009. Seasonality of metazoan ectoparasites in marine European
flounder Platichthys flesus (Teleostei: Pleuronectidae). Parasitology 136(8): 855-865. IF=1.607.
73. Chew, SF, Tng, YYM, Wee, NLJ, Wilson, JM, Ip, YK. 2009. Nitrogen metabolism and branchial
osmoregulatory acclimation in the juvenile marble goby, Oxyeleotris marmorata, exposed to
seawater. Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology
153(3): 360-369. IF=2.196.
74. Chícharo, L, Ben Hamadou, R, Amaral, A, Range, P, Mateus, C, Piló, D, Marques, R, Chícharo, MA.
2009. Application and demonstration of the Ecohydrology approach for the sustainable
functioning of the Guadiana estuary (South Portugal). Ecohydrology and Hidrobiology 9(1): 55-71.
75. Chícharo, L, Faria, A, Morais, P, Amaral, A, Mendes, C, Chicharo, MA. 2009. How to sample larval
fish for taxonomical and ecophysiological studies in shallower temperate coastal ecosystems?.
Les Cahiers de Biologie Marine 50(4): 311-318. IF=0.873.
Hydrozoa) and Palaemon macrodactylus (Crustacea, Decapoda): potential impacts and mitigation
measures. Aquatic Invasions 4(3): 501-506.
77. Cianca, RCC, Barbosa, RD, Faro, LRF, Adan, LV, Gago-Martínez, A, Pallares, MA. 2009. Differential
changes of neuroactive amino acids in samples obtained from discrete rat brain regions after
systemic administration of saxitoxin. Neurochemistry International 54(5-6): 308-313. IF=3.541.
78. Cock, PJA, Antao, T, Chang, JT, Chapman, BA, Cox, CJ, Dalke, A, Friedberg, I, Hamelryck, T, Kauff,
F, Wilczynski, B, de Hoon, MJL. 2009. Biopython: freely available Python tools for computational
molecular biology and bioinformatics. Bioinformatics 25: 1422-1423. IF=4.926.
79. Conceição, LEC, Aragao, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2009. Recent
advances in nutrition of fish larval. Revista Brasileira de Zootecnia 38: 26-35. IF=0.557.
80. Correia, AD, Cunha, SR, Stevens, D. 2009. A behavioural fish model of nociception for testing new
analgesic drugs. Amino Acids 37(S1): 75-75. IF=3.877.
81. Correia, AT, Manso, S, Coimbra, J. 2009. Age, growth and reproductive biology of the European
conger eel (Conger conger Linnaeus, 1758) from the Atlantic Iberian waters. Fisheries Research
99: 196-202. IF=1.531.
82. Costa, G, Santos, MJ, Costa, L, Biscoito, M, Pinheiro de Carvalho, MA, Melo-Moreira, E. 2009.
Helminth parasites from the stomach of conger eel, Conger conger from Madeira Island, Atlantic
Ocean. Journal of Parasitology 95(4): 1013-1015. IF= 1.195.
83. Costa, MC, Santos, ES, Barros, RJ, Pires, C, Martins, M. 2009. Wine wastes as carbon source for
biological treatment of acid mine drainage. Chemosphere 44: 477-480. IF=3.253.
84. Costa, ML, Santos, MCR, Carrapiço, F, Pereira, AL. 2009. Azolla-Anabaena's behaviour in urban
wastewater and artificial media - Influence of combined nitrogen. Water Research 43(15): 37433750. IF=4.355.
85. Costa, R, van Aarle, I, Mendes, R, van Elsas, JD. 2009. Genomics of pyrrolnitrin biosynthetic loci:
evidence for conservation and whole-operon mobility within Gram-negative bacteria.
86. Costas, S, Muñoz Sobrino, C, Alejo, I, Pérez-Arlucea, M. 2009. Holocene evolution of a rockbounded barrier-lagoon system, Cíes Islands, NW Iberia. Earth Surface Processes and Landforms
34: 1575-1586. IF=2.055. (*)
87. Coyer, JA, Hoarau, G, Beszteri, B, Pearson, G, Olsen, JL. 2009. Expressed sequence tag-derived
polymorphic SSR markers of Fucus serratus and amplification in other species of Fucus. Molecular
Ecology Resources 9(1): 168-170. IF=1.251.
88. Cravo, A, Lopes, B, Serafim, A, Company, R, Barreira, L, Gomes, T, Bebianno, MJ. 2009. A
multibiomarker approach in Mytilus galloprovincialis to assess environmental quality. Journal of
Environmental Monitoring 11: 1673-1686. IF=2.225.
89. Cruz, C, Saraiva, A, Santos, MJ, Eiras, JC, Ventura, C, Soares, JP, Hermida, M. 2009. Parasitic
infection levels by Anisakis spp. larvae (Nematoda: Anisakidae) in the black scabbardfish
Aphanopus carbo (Osteichthyes: Trichiuridae) from Portuguese waters. Scientia Marina 73(S2):
115-120. IF=1.174.
90. Cruzeiro, L, Lopes, PA. 2009. Are the native states of proteins kinetic traps?. Molecular Physics
107: 1485-1493. IF=1.634.
91. Cruzeiro, L. 2009. The Davydov/Scott model for energy storage and transport in proteins. Journal
of Biological Physics 35: 43-55. IF=0.646.
92. Cunha, A, Santos, R. 2009. The use of fractal geometry to determine the impact of inlet migration
on the dynamics of a seagrass landscape. Estuarine, Coastal and Shelf Science 84: 584-590.
IF=1.970.
93. Cunha, AH, Araújo, A. 2009. New distribution limits of seagrass beds in West Africa. Journal of
Biogeography 36: 1621-1622. IF=4.087.
94. Cunha, AH, Assis, J, Serrão, E. 2009. Estimation of available seagrass meadow area in Portugal for
transplanting purposes. Journal of Coastal Research 56: 1100-1104. IF=1.366.
95. Cunha, ME, Quental, H, Barradas, A, Pousão-Ferreira, P, Cabrita, E, Engrola, S. 2009. Rearing
larvae of dusky grouper, Epinephelus marginatus (Lowe, 1834), (Pisces: Serranidae) in a semiextensive mesocosm. Scientia Marina 73(S1): 201-212. IF=1.174.
96. Cunha, RL, Grande, C, Zardoya, R. 2009. Neogastropod phylogenetic relationships based on entire
mitochondrial genomes. BMC Evolutionary Biology 9: 210. IF=4.294.
97. Custódio, L, Fernandes, E, Escapa, AL, Aligué, R, Alberício, F, Romano, A. 2009. Antioxidant
activity and in vitro inhibition of tumor cell growth by leaf extracts from the carob tree (Ceratonia
siliqua L.). Pharmaceutical Biology 47: 721-728. IF=0.672.
98. Czerwinski, IA, Erzini, K, Gutiérrez-Estrada, JC, Hernando, JA. 2009. Deep water longline
selectivity for black spot seabream (Pagellus bogaraveo) in the Strait of Gibraltar. Fisheries
Science 75: 285-294. IF=0.684.
99. Davies, SJ, Gouveia, A, Laporte, J, Woodgate, SL, Nate, S. 2009. Nutrient digestibility profile of
premium (category III grade) animal protein by-products for temperate marine fish species
(European sea bass, gilthead sea bream and turbot). Aquaculture Research 40(15): 1759-1769.
IF=1.099.
100. de Montaudouin, X, Thieltges, DW, Gam, M, Krakau, M, Pina, S, Bazairi, H, Dabouineau, L,
Russell-Pinto, F, Jensen KT. 2009. Digenean trematode species in the cockle Cerastoderma edule:
identification key and distribution along the north-eastern Atlantic shoreline. Journal of the
Marine Biological Association of the United Kingdom 89: 543-556. IF=0.995.
101. Deloffre, LAM, Martins, RST, Mylonas, CC, Canario, AVM. 2009. Alternative transcripts of DMRT1
in the European sea bass: Expression during gonadal differentiation. Aquaculture 293: 89-99.
IF=1.925.
102. Dias, J, Conceição, L, Ribeiro, AR, Borges, P, Valente, LMP, Dinis, MT. 2009. Practical diet with low
fish-derived protein is able to sustain growth performance during the grow-out phase in gilthead
seabream (Sparus aurata). Aquaculture 293: 255-262. IF=1.925.
103. Díaz, P, Berecibar, E, Bárbara, I, Cremades, J, Santos, R. 2009. Biology and taxonomic identity of
Erythroglossum lusitanicum (Ceramiales, Rhodophyta) from the Iberian Peninsula. Botanica
Marina 52: 207-216. IF=1.090.
104. Diekmann, OE, Gouveia, L, Serrão, EA, Van de Vliet, MS. 2009. Highly polymorphic microsatellite
markers for the black striped pipefish, Syngnathus abaster. Molecular Ecology Resources 9(6):
1460-1466. IF=1.251.
105. Domingues, I, Guilhermino, L, Soares, AMVM, Nogueira, AJ, Monaghan, KA. 2009. Influence of
exposure scenario on pesticide toxicity in the midge Kiefferutus calligaster (Kieffer).
106. Doropoulos, C, Hyndes, GA, Lavery, PS, Tuya, F. 2009. Dietary preferences of two seagrass
inhabiting gastropods: Allochthonous vs autochthonous resources. Estuarine, Coastal and Shelf
Science 83: 13-18. IF=1.970.
107. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2009. Henneguya corruscans n. sp. (Myxozoa,
Myxosporea, Myxobolidae), a parasite of Pseudoplatystoma corruscans (Osteichthyes,
Pimelodidae) from the Paraná River, Brazil: a morphological and morphometric study. Veterinary
Parasitology 159: 154-158. IF=2.278.
108. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Oudra, B, Del Campo, FF, Vasconcelos, V. 2009.
Compensatory growth induced in zebrafish larvae after pre-exposure to a Microcystis aeruginosa
natural bloom extract containing microcystins. International Journal of Molecular Sciences 10:
133-146. IF=1.387.
109. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2009. Nutritional regulation of hepatic glucose
metabolism in fish. Fish Physiology and Biochemistry 35: 519-539. IF=1.232.
110. Engelen, A, Santos, R. 2009. Which demographic traits determine population growth in the
invasive brown seaweed Sargassum muticum?. Journal of Ecology 97: 675-684. IF=4.690.
excellence. Phycologia 48(4): 29. IF=1.218.
Aquaculture 288: 264-272. IF=1.925.
113. Engrola, S, Mai, M, Dinis, MT, Conceição, LEC. 2009. Co-feeding of inert diet from mouth opening
does not impair protein utilization by Senegalese sole larvae. Aquaculture 287: 185-190.
IF=1.925.
114. Esteves, E, Pina, T, Andrade, J.P. 2009. Diel and seasonal changes in nutritional condition of the
anadromous Twaite shad Alosa fallax fallax (Lacepede, 1803) larvae. Ecology of Freshwater Fish
18: 132-144. IF=1.512.
115. Eynaud, F, de Abreu, L, Voelker, A, Schönfeld, J, Salgueiro, E, Turon, J-L, Penaud, A, Toucanne, S,
Naughton, F, Sanchez Goni, MF, Malaize, B, Cacho, I. 2009. Position of the Polar Front along the
western Iberian margin during key cold episodes of the last 45 ka. Geochemistry, Geophysics and
Geosystems 10: Q07U05. IF=2.626. (*)
116. Faria, AM, Ojanguren, AF, Fuiman, LA, Goncalves, EJ. 2009. Ontogeny of critical swimming speed
of wild-caught and laboratory-reared red drum Sciaenops ocellatus larvae. Marine Ecology
117. Farrell, EJ, Granja, H, Cappietti, L, Ellis, JT, Li, B, Sherman, DJ. 2009. Wave transformation across a
rock platform, Belinho, Portugal. Journal of Coastal Research SI 56: 44-48. IF=1.366.
118. Fernandes, S, Welker, M, Vasconcelos, V. 2009. Changes in the GST activity of the mussel Mytilus
galloprovincialis during exposure and depuration of microcystins. Journal of Experimental
Zoology A - Ecological Genetics and Physiology 311: 226-230. IF=1.444.
Comparative Biochemistry and Physiology C - Toxicology & Pharmacology 149(1): 58-64.
IF=2.582.
120. Figueira, R, Tavares, PC, Palma, L, Beja, P, Sergio, C. 2009. Application of indicator kriging to the
complementary use of bioindicators at three throphic levels. Environmental Pollution 157(10):
2689-2696. IF=3.426.
121. Figueiredo-Silva, AC, Corraze, G, Rema, P, Sanchez-Gurmaches, J, Gutiérrez, J, Valente, LMP.
2009. Blackspot seabream (Pagellus bogaraveo) lipogenic and glycolytic pathways appear to be
more related to dietary protein level than dietary starch type. Aquaculture 291: 101-110.
IF=1.925.
Rachamim, T, Arazi, U, Frenk, S, Korbee, N. 2009. Acclimation of Red Sea macroalgae to solar
radiation: photosynthesis and thallus absorptance. Aquatic Biology 7: 159-172. IF=1.380.
123. Figueroa, FL, Martínez, B, Israel, A, Neori, N, Malta, E-j, Ang, Jr P, Inken, S, Marquardt, R, Korbee,
N. 2009. Effects of nutrient supply on photosynthesis and pigmentation in Ulva lactuca
(Chlorophyta): responses to short-term stress. Aquatic Biology 7: 172-183. IF=1.380.
124. Fontagne, S, Silva, N, Bazin, D, Ramos, A, Aguirre, P, Surget, A, Abrantes, A, Kaushik, SJ, Power,
DM. 2009. Effects of dietary phosphorus and calcium level on growth and skeletal development
in rainbow trout (Oncorhynchus mykiss) fry. Aquaculture 297: 141-150. IF=1.925.
125. Foster, PG, Cox, CJ, Embley, TM. 2009. The primary divisions of Life: a phylogenomic approach
employing composition-heterogeneous methods. Philosophical Transactions of the Royal Society
(B) 364: 2197-2207. IF=5.117.
127. Freitas, PS, Clarke, LJ, Kennedy, H, Richardson, CA. 2009. Ion microprobe assessment of the
heterogeneity of Mg/Ca, Sr/Ca and Mn/Ca ratios in Pecten maximus and Mytilus edulis (bivalvia)
shell calcite precipitated at constant temperature. Biogeosciences 6(7): 1209-1227. IF=3.246. (*)
van der Veer, HW. 2009. Reconstruction of food conditions for Northeast Atlantic bivalve species
based on Dynamic Energy Budgets. Journal of Sea Research 62: 75-82. IF=1.803.
and distribution of juvenile flounder, Platichthys flesus, in Minho estuary (NW Iberian Peninsula).
Aquatic Ecology 43(4): 1143-1153. IF=1.549.
130. Frija, LMT, Fausto, R, Loureiro, RMS, Cristiano, MLS. 2009. Synthesis and structure of novel
benzisothiazole-tetrazolyl derivatives for potential application as nitrogen ligands. Journal of
Molecular Catalysis A - Chemical 305(S1): 142-146. IF=3.135.
131. Gabriel, D, Schils, T, Neto, AI, Paramio, L, Fredericq, S. 2009. Predaea feldmannii subsp azorica
(Nemastomataceae, Nemastomatales), a new subspecies of red algae (Rhodophyta) from the
Azores. Cryptogamie Algologie 30(3): 251-270. IF=0.574.
senegalensis, Kaup 1858): evaluation in larval and postlarval stages. Aquaculture Research
40(14): 1585-1593. IF=1.099.
133. Gherardi J-M, Labeyrie, L, Nave, S, Francois, R, McManus, JF, Cortijo, E. 2009. Glacial-interglacial
circulation changes inferred from 231 Pa/ 230 The sedimentary record in the North Atlantic
region. Paleoceanography 24: PA2204. IF=3.644. (*)
134. Gil, I, Keigwin, L, Abrantes, F. 2009. Deglacial diatom productivity and surface ocean properties
over the Bermuda Rise, northeast Sargasso Sea. Paleoceanography 24: PA4101. IF=3.644.
135. Gomez-Zavaglia, A, Kaczor, A, Almeida, R, Cristiano, MLS, Eusebio, MÊS, Maria, TMR, Mobili, P,
Fausto, R. 2009. Thermally induced sigmatropic isomerization of pseudosaccharyl allylic ether.
Journal of Physical Chemistry A 113(15): 3517-3522. IF=2.899.
136. Gómez-Zavaglia, A, Kaczor, A, Coelho, D, Cristiano, MLS, Fausto, R. 2009. Conformational and
structural analysis of 2-allyl-1,2-benzisothiazol-3(2H)-one 1,1-dioxide as probed by matrixisolation spectroscopy and quantum chemical calculations. Journal of Molecular Structure 919:
271-276. IF=1.551.
137. Gomez-Zavaglia, A, Reva, ID, Frija, LMT, Cristiano, MLS, Fausto, R. 2009. Photochemistry of
tetrazole derivatives in cryogenic rare gas matrices. Chemical Physics Research Journal 1(4): 221250.
138. Gonzaléz-Wanguemert, M, Cánovas, F, Marcos, C, Pérez-Ruzafa, A. 2009. Phosphoglucose
isomerase variability of Cerastoderma glaucum as a model for testing the influence of
environmental conditions and dispersal patterns through quantitative ecology approaches.
Biochemical Systematics and Ecology 37: 325-333. IF=1.131.
139. Gordo, LS, Baptista, I, Carvalho, L, Costa, V, Cruz, C, Eiras, JC, Farias, I, Figueiredo, I, Loureno, H,
Bordalo-Machado, P, Neves, A, Nunes, ML, Rei, S, Santos, MJ, Saraiva, A, Vieira, AR. 2009. Stock
structure of black scabbardfish (Aphanopus carbo Lowe, 1839) in the southern North East
Atlantic. Scientia Marina 73(S2): 89-101. IF=1.174.
140. Gouveia, KG, Ferreira, PG, Roque da Costa, JC, Vaz-Pires, P, Martins da Costa, P. 2009.
Assessment of the efficiency of captive-bolt stunning in cattle and feasibility of associated
behavioural signs. Animal Welfare 18: 171-175. IF=1.209.
141. Gouveia, KG, Vaz-Pires, P, Martins da Costa, P. 2009. Welfare assessment of broilers through
examination of haematomes, foot pad dermatitis, scratches and breast blisters at processing.
Animal Welfare 18: 43-48. IF=1.209.
142. Gravato, C, Guilhermino, L. 2009. Effects of benzo(a)pyrene on sea bass (Dicentrarchus labrax L.):
from biomarkers to effects on growth, feeding and behavior. Human and Ecological Risk
Assessment 15: 121-137. IF=1.528.
143. Gravel, A, Wilson, JM, Neto Pedro, DF, Vijayan, MM. 2009. Non-steroidal anti-inflammatory drugs
disturb the osmoregulatory, metabolic and cortisol responses associated with seawater exposure
in rainbow trout. Comparative Biochemistry and Physiology C - Toxicology & Pharmacology
149(4): 481-490. IF=2.582.
144. Guimarães, L, Gravato, C, Santos, J, Monteiro, LS, Guilhermino, L. 2009. Yellow eel (Anguilla
anguilla) development in NW Portuguese estuaries with different contamination levels.
Ecotoxicology 18: 385-402. IF=3.507.
145. Hadorn, R, Afonso, CML, Rolán, E. 2009. A new Fusinus (Gastropoda: fasciolariidae) from the
Algarve, south coast of Portugal. Iberus 27(1): 119-129.
146. Hardoim, CCP, Costa, R, Araújo, FV, Hajdu, E, Peixoto, R, Lins, U, Rosado, AS, van Elsas, JD. 2009.
Bacterial diversity in the marine sponge Aplysina fulva in Brazilian coastal waters. Applied and
147. Howcroft, CF, Amorim, MJB, Gravato, C, Guilhermino, L, Soares, AMVM. 2009. Effects of natural
and chemical stressors on Echytraeus albidus: can oxidative stress parameters be used as fast
screening tools for the assessment of different stress impacts in soils?. Environment International
35: 318-324. IF=4.786.
148. Huertas, M, Hagey, L, Hofmann, AF, Cerdà, J, Canário, AVM, Hubbard, PC. 2009. Olfactory
sensitivity to bile fluid and bile salts in the European eel (Anguilla anguilla), goldfish (Carassius
auratus) and Mozambique tilapia (Oreochromis mossambicus) suggests a 'broad range' sensitivity
not confined to those produced by con-specifics alone. Journal of Experimental Biology 213: 308317. IF=2.722.
149. Jordanova, M, Miteva, N, Santos, N, Malhão, F, Rocha, E. 2009. Crystalline inclusions in the liver
of wild female Ohrid trout (Salmo letnica Kar.). Tissue & Cell 41: 281-285. IF=1.011.
150. Kadar, E, Lobo-da-Cunha, A, Azevedo, C. 2009. Mantle-to-shell CaCO3 transfer during shell repair
at different hydrostatic pressures in the deep-sea vent mussel Bathymodiolus azoricus (Bivalvia:
Mytilidae). Marine Biology 156: 959-967. IF=1.999.
151. Kalamarz, H, Nietrzeba, M, Fuentes, J, Martinez-Rodriguez, G, Miguel Mancera, J, Kulczykowska,
E. 2009. Melatonin during larval and post-larval development of gilthead sea bream Sparus
auratus L.: more than time-keeping molecule? Journal of Fish Biology 75: 142-155. IF=1.226.
152. Kolmakov, NN, Hubbard, PC, Lopes, O, Canario, AVM. 2009. Effect of acute copper sulfate
exposure on olfactory responses to amino acids and pheromones in goldfish (Carassius auratus).
Environmental Science and Technology 43(21): 8393-8399. IF=4.630.
aurata) to in vivo exposure to a mix of selected PAHs. Ecotoxicology and Environmental Safety
72(4): 1296-1302. IF=2.133.
aurata) to in vivo exposure to pyrene and fluorene. Journal of Experimental Marine Biology and
Ecology 372: 49-57. IF=2.116.
155. Kortner, TM, Rocha, E, Arukwe, A. 2009. Androgenic modulation of early growth of Atlantic cod
(Gadus morhua L.) previtellogenic oocytes and zona radiata-related genes. Journal of Toxicology
and Environmental Health - Part A 72: 184-195. IF=1.724.
156. Kortner, TM, Rocha, E, Arukwe, A. 2009. Previtellogenic oocyte growth and transcriptional
changes of steroidogenic enzyme genes in immature female Atlantic cod (Gadus morhua L.) after
exposure to the androgens 11-ketotestosterone and testosterone. Comparative Biochemistry
and Physiology A - Molecular & Integrative Physiology 152: 304-313. IF=2.196.
157. Kovitvadhi, S, Kovitvadhi, U, Sawangwong, P, Trisaranuwatana, P, Machado, J. 2009.
Morphometric relationship of weight and size of cultured freshwater pearl mussel, Hyriopsis
(Limnoscapha) myersiana, under laboratory conditions and earthen pond phases. Aquaculture
International 17: 57-67. IF=0.753.
158. Laiz-Carrion, R, Fuentes, J, Redruello, B, Guzman, JM, del Rio, MPM, Power, D, Mancera, JM.
2009. Expression of pituitary prolactin, growth hormone and somatolactin is modified in
response to different stressors (salinity, crowding and food-deprivation) in gilthead sea bream
Sparus auratus. General and Comparative Endocrinology 162(3): 293-300. IF= 2.732.
159. Laize, V, Tiago, DM, Aureliano M, Cancela, ML. 2009. New insights into mineralogenic effects of
vanadate. Cellular and Molecular Life Sciences 66(24): 3831-3836. IF=6.090.
Ectocarpus siliculosus and Fucus vesiculosus: further insights on the evolution of red-algal derived
plastids. BMC Evolutionary Biology 9: 253. IF=4.294.
161. Leão, P, Vasconcelos, MTSD, Vasconcelos, VM. 2009. Allelopathy in freshwater cyanobacteria.
Critical Reviews in Microbiology 35(4): 271-282. IF=3.207.
green microalgae at low cell densities. European Journal of Phycology 44(3): 347-355. IF=2.270.
163. Lebreiro, SM, Voelker, AHL, Vizcaino, A, Abrantes, FG, Alt-Epping, U, Jung, S, Thouveny, N, Gracia,
E. 2009. Sediment instability on the Portuguese continental margin under abrupt glacial climate
changes (last 60 kyr). Quaternary Science Reviews 28: 3211-3223. IF=4.245. (*)
164. Lefrancois, C, Ferrari, RS, da Silva, JM, Domenici, P. 2009. The effects of progressive hypoxia on
spontaneous activity in single and shoaling golden grey mullet Liza aurata. Journal of Fish Biology
75(7): 1615-1625. IF=1.226.
165. Leitao, A, Vasconcelos, P, Ben-Hamadou, R, Gaspar, MB, Barroso, CM, Ruano, F. 2009.
Cytogenetics of Bolinus brandaris and phylogenetic inferences within the Muricidae (Mollusca:
Gastropoda). Biological Journal of the Linnean Society 96: 185-193. IF=2.040.
166. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2009. Diplodus spp. assemblages on artificial reefs:
importance for near shore fisheries. Fisheries Management and Ecology 16: 88-99. IF=1.264.
167. Leliaert, F, Zhang, XW, Ye, NH, Malta, E, Engelen, AH, Mineur, F, Verbruggen, H, De Clerck, O.
2009. Research note: Identity of the Qingdao algal bloom. Phycological Research 57: 147-151.
IF=1.246.
168. León-Cisneros, K, Riosmena-Rodríguez, R, Neto, AI, Hernández-Carmona, G. 2009. The red algal
genus Scinaia (Nemaliales; Rhodophyta) on the Gulf of California, Mexico: a taxonomic account.
Phycologia 48(3): 186-210. IF=1.218.
169. Li, B, Granja, HM, Farrell, EJ, Ellis, JT, Sherman DJ. 2009. Aeolian saltation at Esposende Beach,
Portugal. Journal of Coastal Research SI 56: 327-331. IF=1.366.
170. Lobo-da-Cunha, A, Ferreira, I, Coelho, R, Calado, G. 2009. Light and electron microscopy study of
the salivary glands of the carnivorous opisthobranch Philinopsis depicta (Mollusca, Gastropoda).
Tissue & Cell 41: 367-375. IF=1.011.
171. Lopes, C, Mix, AC. 2009. Pleistocene megafloods in the northeast Pacific. Geology 37(1): 79-82.
IF=4.368. (*)
172. Lopes-Lima, M, Lopes, A, Casaca, P, Nogueira, I, Checa, A, Machado, J. 2009. Seasonal variations
2+
of pH, pCO2, pO2, HCO3 and Ca in the haemolymph: implications on the calcification physiology
in Anodonta cygnea. Journal of Comparative Physiology B - Biochemical Systemic and
Environmental Physiology 179: 279-286. IF=1.607.
173. Machado, J, Lopes-Lima, M, Damasceno-Oliveira, A, Colaco, A, Andrade, J, Silva, D, JimenezLopez, C, Rodriguez-Navarro, A, Checa, A. 2009. The influence of hydrostatic pressure on shell
mineralization of Anodonta cygnea: a comparative study with a hydrothermal vent bivalve
Bathymodiolus azoricus. Journal of Shellfish Research 2(4): 899-904. IF=0.891.
174. Madureira, T, Barreto, J, Rocha, MJ, Cass, Q, Tiritan, ME. 2009. Pharmaceutical trace analysis in
aqueous environmental matrices by liquid chromatography-ion trap mass spectrometry. Journal
of Chromatography A 1216: 7033-7042. IF=4.101.
175. Magalhães, C, Machado, A, Bordalo, AA. 2009. Temporal variability of relative abundance of
ammonia oxidizing bacteria vs archaea in the sandy flat of the Douro River estuary, Portugal.
Aquatic Microbial Ecology 56: 13-23. IF=1.743.
176. Maggi, E, Bertocci, I, Vaselli, S, Benedetti-Cecchi, L. 2009. Effects of changes in number, identity
and abundance of habitat-forming species on assemblages of rocky seashores. Marine Ecology
177. Mai, MG, Engrola, S, Morais, S, Portella, MC, Verani, JR, Dinis, MT, Conceicão, LE. 2009. Cofeeding of live feed and inert diet from first-feeding affects Artemia lipid digestibility and
retention in Senegalese sole (Solea senegalensis) larvae. Aquaculture 296: 284-291. IF=1.925.
178. Makridis, P, Moreira, C, Costa, RA, Rodrigues, P, Dinis, MT. 2009. Use of microalgae
bioencapsulated in Artemia during the weaning of Senegalese sole (Solea senegalensis Kaup).
Aquaculture 292: 153-157. IF=1.925.
179. Marcos, R, Malhão, F, Monteiro, R, Rocha, E. 2009. Gender and aging in the liver: preliminary
data using design-based stereological methods. Microscopy and Microanalysis 15 (S3): 45-46.
IF=3.035.
180. Marcos, R, Santos, M, Santos, N, Malhão, F, Ferreira, F, Monteiro, RAF, Rocha, E. 2009. Use of
destained cytology slides for the application of routine special stains. Veterinary Clinical
Pathology 38: 94-102. IF=0.793.
mechanisms of BMP2. FEBS Journal 276: 111. IF=3.042.
182. Marques, JF, Santos, MJ, Cabral, HN. 2009. Zoogeographical patterns of flatfish
(Pleuronectiformes) parasites in the northeast Atlantic and the importance of the Portuguese
coast as a transitional area. Scientia Marina 73(3): 461-471. IF=1.174.
183. Martinez-Paramo, S, Barbosa, V, Perez-Cerezales, S, Robles, V, Herraez, MP. 2009. Cryoprotective
effects of antifreeze proteins delivered into zebrafish embryos. Cryobiology 58(2): 128-133.
IF=1.718.
184. Martins da Costa, P, Belo, A, Gonçalves, J, Bernardo, F. 2009. Field trial evaluating changes in
prevalence and patterns of antimicrobial resistance among Escherichia coli and Enterococcus spp.
isolated from growing broilers medicated with enrofloxacin, apramycin and amoxicillin.
Veterinary Microbiology 139: 284-292. IF=2.874.
185. Martins, DA, Valente, LMP, Lall, S. 2009. Apparent digestibility of lipid and fatty acids in fish oil,
poultry fat and vegetable oil diets by Atlantic halibut, Hippoglossus hippoglossus L. Aquaculture
294: 132-137. IF=1.925.
186. Martins, GM, Amaral, AF, Wallenstein, FM, Neto, AI. 2009. Influence of a breakwater on nearby
rocky intertidal community structure. Marine Environmental Research 67: 237-245. IF=1.755.
187. Martins, J, Saker, M, Moreira, C, Welker, M, Fastner, J, Vasconcelos, V. 2009. Peptide diversity in
strains of the cyanobacterium Microcystis aeruginosa isolated from Portuguese water supplies.
Applied Microbiology and Biotechnology 82(5): 951-961. IF=2.896.
188. Martins, JC, Leão, P, Vasconcelos, VM. 2009. Differential protein expression in Corbicula fluminea
upon exposure to a Microcystis aeruginosa toxic strain. Toxicon 53: 409-416. IF=2.128.
189. Martins, JC, Vasconcelos, VM. 2009. Microcystin dynamics in aquatic organisms. Journal of
Toxicology and Environmental Health - Part B 12: 65-82. IF=3.617.
190. Martins, M, Faleiro, ML, Barros, RJ, Veríssimo, AR, Barreiros, MA, Costa, MC. 2009.
Characterization and activity studies of highly heavy metal resistant sulphate-reducing bacteria.
Journal of Hazardous Materials 166: 706-713. IF=4.144.
191. Martins, M, Faleiro, ML, Barros, RJ, Veríssimo, AR, Costa, MC. 2009. Biological sulphate reduction
using food industry wastes as carbon sources. Biodegradation 20(4): 559-567. IF=1.873.
2+
192. Martins, RST, Fuentes, J, Almeida, O, Power, DM, Canario, AVM. 2009. Ca -Calmodulin
regulation of testicular androgen production in Mozambique tilapia (Oreochromis mossambicus).
General and Comparative Endocrinology 162(2): 153-159. IF=2.732.
193. Massa, SI, Arnaud-Haond, S, Pearson, GA, Serrão, EA. 2009. Temperature tolerance and survival
of intertidal populations of the seagrass Zostera noltii (Hornemann) in Southern Europe (Ria
Formosa, Portugal). Hydrobiologia 619: 195-201. IF=1.754.
194. Mendonça, VM, Raffaelli, DG, Boyle, PR, Emes, E. 2009. Trophodynamics in a shallow lagoon off
Northwestern Europe (Culbin Sands, Moray Firth): spatial and temporal variability of epibenthic
communities, their diets, and consumption efficiency. Zoological Studies 48(2): 196-214.
IF=0.860.
195. Mesquita, R, Ferreira, T, Segundo, R, Teixeira, C, Bordalo, AA, Rangel, AOSS. 2009. Development
of a sequential injection system for the determination of nitrite and nitrate in waters with
different salinity: application to estuaries in NW Portugal. Analytical Methods 1: 195-202.
196. Mil-Homens, M, Branco, V, Lopes, C, Vale, C, Abrantes, F, Boer, W, Vicente, M. 2009. Using factor
analysis to characterise historical trends of trace metal contamination in a sediment core from
the Tagus Prodelta, Portugal. Water Air and Soil Pollution 197: 277-287. IF=1.676. (*)
197. Mil-Homens, M, Branco, V, Lopes, C, Vale, C, Boer, W, Alt-Epping, U, Abrantes, F, Vicente, M.
2009. Sedimentary record of anthropogenic metal inputs in the Tagus prodelta (Portugal).
Continental Shelf Research 29: 381-392. IF=2.183. (*)
198. Monteiro, C, Engelen, AH, Santos, R. 2009. Macro- and mesoherbivores prefer native seaweeds
over the invasive brown seaweed Sargassum muticum: a potential regulating role on invasions.
199. Monteiro, C, Engelen, AH, Serrão, EA, Santos, R. 2009. Habitat differences in the timing of
reproduction of the invasive alga Sargassum muticum (Phaeophyta, Sargassaceae) over tidal and
200. Monteiro, SM, Rocha, E, Mancera, JM, Fontaínhas-Fernandes, A, Sousa, M. 2009. A stereological
study of copper toxicity in gills of Oreochromis niloticus. Ecotoxicology and Environmental Safety
72: 213-23. IF=2.133.
201. Morais, P, Chicharo, MA, Chicharo, L. 2009. Changes in a temperate estuary during the filling of
the biggest European dam. Science of the Total Environment 407: 2245-2259. IF=2.905.
202. Morais, P, Faria, A, Chicharo, MA, Chicharo, L. 2009. The unexpected occurrence of late Sardina
pilchardus (Walbaum, 1792) (Osteichthyes: Clupeidae) larvae in a temperate estuary. Les Cahiers
de Biologie Marine 50(1): 79-89. IF=0.873.
203. Morais, S, Conceicão, LEC. 2009. A new method for the study of essential fatty acid requirements
in fish larvae. British Journal of Nutrition 101: 1564-1568. IF=3.446.
204. Moravec, F, Saraiva, A, Abdullah, SMA, Bilal, SJ, Rahemo, ZIF. 2009. Observations on
Rhabdochona spp. (Nematoda: Rhabdochonidae) parasitizing cyprinid fishes in Iraq, with a
redescription of Rhabdochona tigridis Rahemo, 1978. Systematic Parasitology 74: 125-135.
IF=0.911.
205. Moreira-Silva, J, Coimbra, JC, Wilson, JM. 2009. Ammonia sensitivity of the glass eel (Anguilla
+ +
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anguilla L.): Salinity dependence and the role of branchial Na /K (NH4 )-ATPase. Environmental
Toxicology & Chemistry 28: 141-147. IF=2.565.
206. Morgado, I, Campinho, MA, Costa, R, Jacinto, R, Power, DM. 2009. Disruption of the thyroid
system by diethylstilbestrol and ioxynil in the sea bream (Sparus aurata). Aquatic Toxicology
92(4): 271-280. IF= 3.124.
207. Nair, G, Babu, DS, Damodaran, KT, Shankar, R, Prabhu, CN. 2009. Weathering of Ilmenite from
Chavara deposit and its comparison with Manavalakurichi placer ilmenite, southwestern India.
Journal of Asian Earth Sciences 34: 115-122. IF=1.842. (*)
208. Naughton, F, Sánchez Goñi, MF, Kageyama, M, Bard, E, Cortijo, E, Desprat, S, Duprat, J, Malaizé,
B, Joli, C, Rostek, F, Turon, J-L. 2009. Wet to dry climatic trend in north western Iberia within
Heinrich events. Earth and Planetary Science Letters 284: 329-342. IF=4.062. (*)
209. Neves, J, Wilson, JM, Rodrigues, P. 2009. Transferrin and ferritin response to bacterial infection:
the role of the liver and brain in fish. Developmental and Comparative Immunology 33(7): 848857. IF=3.290.
210. Olivar, PM, Diaz, MV, Chicharo, MA. 2009. Tissue effect on RNA:DNA ratios of marine fish larvae.
Scientia Marina 73(S1): 171-182. IF=1.174.
211. Oliveira, C, Dinis, MT, Soares, F, Cabrita, E, Pousão-Ferreira, P, Sanchez-Vazquez, FJ. 2009. Lunar
and daily spawning rhythms of Senegal sole Solea senegalensis. Journal of Fish Biology 75: 61-74.
IF=1.226.
212. Oliveira, F, Almeida, C, Gonçalves, JMS. 2009. Ascidonia flavomaculata (Heller, 1864), a new
record for the Portuguese marine invertebrate fauna (Decapoda, Pontoniidae). Crustaceana
82(1): 63-67. IF=0.507.
213. Oliveira, RF, Silva, A, Canario, AVM. 2009. Why do winners keep winning? Androgen mediation of
winner but not loser effects in cichlid fish. Proceedings of the Royal Society B - Biological Sciences
276: 2249-2256. IF=4.857.
214. Ortega, L, Tuya, F, Haroun, RJ. 2009. El erizo de mar Diadema antillarum Phillipi, 1845 influye
sobre la diversidad y composición de la comunidad de mega-invertebrados vágiles en fondos
rocosos del Archipiélago Canario. Revista de Biología Marina y Oceanografía 44(2): 489-495.
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216. Osswald, J, Rellan, S, Gago, A, Vasconcelos, VM. 2009. Production of anatoxin-a by cyanobacteria
strains isolated from Portuguese fresh waters. Ecotoxicology 18: 1110-1115. IF=3.507.
217. Oudra, B, Dadi-El Andaloussi, M, Vasconcelos, VM. 2009. Identification and quantification of
microcystins from a Nostoc bloom occurring in Oukaïmeden River (High-Atlas Mountains of
Marrakesh, Morocco). Environmental Monitoring and Assessment 149: 437-444. IF=1.356.
218. Ozório, ROA, Andrade, C, Conceição, LEC, Timóteo, VMFA, Valente, LMP. 2009. Effects of feeding
levels on growth response, body composition, and energy expenditure in blackspot seabream,
Pagellus bogaraveo, juveniles. Journal of the World Aquaculture Society 40: 95-103. IF=0.780.
219. Ozorio, ROA, Valente, LMP, Correia, S, Pousao-Ferreira, P, Damasceno-Oliveira, A, Escorcio, C,
Oliva-Teles, A. 2009. Protein requirement for maintenance and maximum growth of two-banded
seabream (Diplodus vulgaris) juveniles. Aquaculture Nutrition 15(1): 85-93. IF=1.482.
220. Ozório, ROA. 2009. Dietary L-carnitine supplementation to cultivated fish: A mini-review. Current
Nutrition & Food Science 5: 40-48.
221. Palma, J, Bureau, DP, Correia, M, Andrade, JP. 2009. Effect of temperature, density and early
weaning in survival and growth of Palaemonetes varians larvae. Aquaculture Research 40(13):
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222. Passos, ALS, Pinto, PIS, Power, DM, Canario, AVM. 2009. A yeast assay based on the gilthead sea
bream (teleost fish) estrogen receptor beta for monitoring estrogen mimics. Ecotoxicology and
Environmental Safety 72(5): 1529-1537. IF=2.133.
223. Pearson, GA, Lago-Leston, A, Mota, C. 2009. Frayed at the edges: Selective pressure and adaptive
response to abiotic stressors are mismatched in low diversity edge populations. Journal of
Ecology 97: 450-462. IF=4.690.
224. Peh, WYX, Chew, SF, Wilson, JM, Ip, YK. 2009. Branchial and intestinal osmoregulatory
acclimation in the four-eyed sleeper, Bostrychus sinensis (Lacepède), exposed to seawater.
225. Pereira, AL, Carrapiço, F. 2009. Culture of Azolla filiculoides in artificial conditions. Plant
Biosystems 143: 431-434. IF=0.744.
226. Pereira, AL, Figueiredo, AC, Barroso, JG, Pedro, LG, Carrapiço, F. 2009. Volatile compounds from
the symbiotic system Azolla filiculoides - Anabaena azollae bacteria. Plant Biosystems 143: 268274. IF=0.744.
227. Pereira, MJ, Carvalho, E, Eriksson, JW, Crans, DC, Aureliano, M. 2009. Effects of decavanadate
and insulin enhancing vanadium compounds on glucose uptake in isolated rat adipocytes. Journal
of Inorganic Biochemistry 103(12): 1687-1692. IF=3.252.
228. Pereira, MJ, Nunes, P, Palming, J, Eriksson, J, Aureliano, MA, Carvalho, E. 2009. Effects of
immunosuppresive agents on insulin stimulated glucose transport in primary rat adipocytes.
Diabetologia 52: S275-275. IF=6.551.
229. Pereira, S, Saker, M, Vale, M, Vasconcelos, VM. 2009. Comparison of sensitivity of grasses (Lolium
perenne L. and Festuca rubra L.) and lettuce (Lactuca sativa L.) exposed to water contaminated
with microcystins. Bulletin of Environmental Contamination and Toxicology 83: 81-84. IF=0.992.
230. Peres, H, Oliva-Teles, A. 2009. The optimum dietary essential amino acid profile for gilthead
seabream (Sparus aurata) juveniles. Aquaculture 296: 81-86. IF=1.925.
231. Perez-Cerezales, S, Martinez-Paramo, S, Cabrita, E, Martinez-Pastor, F, Herraez, MP. 2009.
Evaluation of oxidative DNA damage promoted by storage in sperm from sex-reversed rainbow
trout. Theriogenology 71: 605-613. IF=2.073.
histopathological and molecular data. Journal of Parasitology 95(2): 353-359. IF=1.195.
233. Pina, S, Tajdari, J, Russell-Pinto, F, Rodrigues, P. 2009. Morphological and molecular studies on
life cycle stages of Diphtherostomum brusinae (Digenea: Zoogonidae) from northern Portugal.
Journal of Helminthology 83: 321-331. IF=0.863.
234. Pinheiro, P, Downie, H, Fuentes, J, Power, DM, Canario, AVM. 2009. Functional activity and gene
expression of parathormone. The Journal of Physiological Sciences 59 (S1): 273. IF=1.125.
235. Pinto, PIS, Estevao, MD, Redruello, B, Socorro, SM, Canario, AVM, Power, DM. 2009.
Immunohistochemical detection of estrogen receptors in fish scales. General and Comparative
Endocrinology 160(1): 19-29. IF= 2.732.
236. Pinto, PIS, Power, DM, Canario, AVM, Thorne, MAS, Reinhardt, R, Matsumura, H, Terauchi, R.
2009. Transcriptome profiling of the gills of a euryhaline teleost fish, Tetraodon nigroviridis, in
response to altered calcium concentrations in water. Comparative Biochemistry and Physiology A
- Molecular & Integrative Physiology 154(1): S2-S3. IF=2.196.
237. Pinto, W, Figueira, L, Dinis, MT, Aragão, C. 2009. How does fish metamorphosis affect aromatic
amino acid metabolism?. Amino Acids 36: 177-183. IF=3.877.
238. Pullin, A, Báldi, A, Can, O, Dieterich, M, Kati, V, Livoreil, B, Lövei, G, Nevin, O, Selva, N, SousaPinto, I. 2009. Conservation focus on Europe: major conservation policy issues that need to be
informed by conservation science. Conservation Biology 23: 818-824. IF=4.666.
239. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2009. Bone-related role of the LIM-only protein FHL2
in fish. Bone 44: S311-S312. IF=4.089.
240. Ramírez, R, Tuya, F, Haroun, RJ. 2009. Efectos potenciales del marisqueo sobre moluscos
gasterópodos de interés comercial (Osilinus spp. y Patella spp.) en el Archipiélago Canario.
Revista de Biología Marina y Oceanografía 44(3): 703-714. IF=0.361.
241. Ramos, A, Marques, A, Rodrigues, M, Henriques, N, Baumgartner, A, Castilho, R, Brenig, B,
Varela, J. 2009. Molecular and functional characterization of a cDNA encoding 4-hydroxy-3methylbut-2-enyl diphosphate reductase from Dunaliella salina. Journal of Plant Physiology 166:
968-977. IF=2.500.
242. Ramos, S, Duarte, RO, Moura, JJG, Aureliano, M. 2009. Decavanadate interactions with actin:
cysteine oxidation and vanadyl formation. Dalton Transactions 38: 7985-7994. IF=4.081.
243. Ramos, S, Duarte, RO, Moura, JJG, Aureliano, M. 2009. Decavanadate promotes actin cysteine
oxidation trough vanadyl formation. FEBS Journal 276(S1): 162-162. IF=3.042.
244. Ramos, S, Ré, P, Bordalo, AA. 2009. Environmental control on early life stages of flatfishes in the
Lima Estuary (NW Portugal). Estuarine, Coastal and Shelf Science 83: 252-264. IF=1.970.
245. Ramos, S, Ré, P, Bordalo, AA. 2009. New insights into the early life ecology of Sardina pilchardus
(Walbaum, 1792) in the northern Iberian Atlantic. Scientia Marina 73: 449-459. IF=1.174.
246. Rangel, L, Santos, MJ, Cech, G, Czeckely, C. 2009. Morphology, molecular data and development
of Zschokkela mugilis (Myxosporea, Bivalvulida) in a polychaete alternate host, Nereis
diversicolor. Journal of Parasitology 95(3): 561-569. IF=1.195.
247. Rangel, L, Santos, MJ. 2009. Diopatra neapolitana (Polychaeta: Onuphidae) as a second
intermediate host of Gymnophallus choledochus (Digenea: Gymnophallidae) in the Aveiro Estuary
(Portugal): distribution within the host and histopathology. Journal of Parasitology 95(5): 12331236. IF=1.195.
248. Ratkai, C, Quinteira, S, Grosso, F, Monteiro, N, Nagy, E, Peixe, L. 2009. Controlling for false
positives: interpreting MBL Etest and MBL combined disc test for the detection of metallo-betalactamases. Journal of Antimicrobial Chemotherapy 63(3): 657-658. IF=4.352.
249. Rato, M, Russell-Pinto, F, Barroso, C. 2009. Assessment of digenean parasitism in Nassarius
reticulatus (L.) along the Portuguese coast: evaluation of possible impacts on reproduction and
imposex expression. Journal of Parasitology 95: 327-336. IF=1.195.
250. Regueiras, A, Saker, M, Vasconcelos, V. 2009. Use of PCR for the detection of pathogenic bacteria
and cyanobacteria in water samples from different urban water sources (Porto, Portugal).
Fresenius Environmental Bulletin 18(12): 2365-2365. IF=0.531.
251. Reis, PA, Antunes, JC, Almeida, CMR. 2009. Metal levels in sediments from the Minho estuary salt
marsh: a clean área?. Environmental Monitoring and Assessment 159: 191-205. IF=1.356.
252. Reis-Henriques, MA, Ferreira, M, Coimbra, AM, D'Silva, C, Costa, J, Shailaja, MS. 2009.
Phenanthrene and nitrite effects on juvenile sea bass, Dicentrarchus labrax, using hepatic
biotransformation enzymes, biliary fluorescence and micronuclei as biomarkers. Ciencias Marinas
35: 29-40. IF=0.655.
253. Rellan, S, Osswald, J, Saker, M, Gago, A, Vasconcelos VM. 2009. First detection of anatoxin-a in
human and animal dietary supplements containing cyanobacteria. Food and Chemical Toxicology
47(9): 2189-2195. IF=2.114.
254. Rema, P, Martins, J. 2009. Growth and survival of Carassius auratus larvae fed commercial micro
diets. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61(3): 714-720. IF=0.349.
255. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Franquinho, F, Monteiro, RAF, Rocha, E. 2009.
Histological and stereological characterization of brown trout (Salmo trutta) trunk kidney.
Microscopy and Microanalysis 15(S3): 1-2. IF=3.035.
256. Ribeiro, C, Pardal, MA, Martinho, F, Margalho, R, Tiritan, ME, Rocha, E, Rocha, MJ. 2009.
Distribution of endocrine disruptors in the Mondego River estuary (Portugal). Environmental
257. Ribeiro, C, Pardal, MA, Tiritan, ME, Rocha, E, Rocha, MJ. 2009. Spatial distribution and
quantification of endocrine disrupting chemicals in Sado River estuary by solid phase extraction
and high-performance liquid chromatography with diode-array detection. Environmental
258. Ribeiro, C, Tiritan, ME, Rocha, E, Rocha, MJ. 2009. Seasonal and spatial distribution of several
endocrine disrupting compounds in the Douro River Estuary, Portugal. Archives of Environmental
Contamination and Toxicology 56: 1-11. IF=1.743.
259. Robalo, JI, Sousa-Santos, C, Cabral, H, Castilho, R, Almada, VC. 2009. Genetic evidence fails to
discriminate between Macroramphosus gracilis Lowe, 1839 and M. scolopax Linnaeus, 1758 in
Portuguese waters. Marine Biology 156(8): 1733-1737. IF=1.999.
260. Roberto, VP, Gavaia, PJ, Viegas, CSB, Ortiz Delgado, JB, Sarasquete, C, Cancela, ML. 2009.
Molecular cloning of matrix Gla protein from turbot (Scophthalmus maximus): gene expression
analysis and identification of sites of protein accumulation. Aquaculture 294: 202-211. IF=1.925.
261. Rocha, E, Rocha, MJ, Galante, MH, Silva, MW, Monteiro, RAF. 2009. The hepatocytes of the
brown trout (Salmo trutta fario): A stereological study of their number and size during the
breeding cycle. Ichthyological Research 56: 43-54. IF=0.635.
262. Rodrigues, P, Silva, T, Jessen, F, Dias, J. 2009. On the reproducibility of a fractionation procedure
for fish muscle proteomics. Molecular & Cellular Proteomics 8: S19. IF=8.791.
263. Rodrigues, T, Grimalt, JO, Abrantes, F, Flores, JA, Lebreiro, S. 2009. Interdependences of changes
in sea surface temperature, productivity and fluvial inputs in the continental shelf of the Iberian
margin (Tagus mud patch) during the Holocene. Geochemistry, Geophysics and Geosystems 10:
Q07U06. IF=2.626. (*)
264. Rosas-Alquicira, EF, Neto, AI, Riosmena-Rodríguez, R, Couto, RP. 2009. New additions to the
Azorean algal flora, with ecological observations on rhodolith formations. Cahiers de Biologie
Marine 50: 143-151. IF=0.873.
265. Rubal, M, Guilhermino, L, Medina, MH. 2009. Individual, population and community level effects
of subtle anthropogenic contamination in estuarine meiobenthos. Environmental Pollution 157:
2751-2758. IF=3.426.
267. Runcie, JW, Paulo, D, Santos, R, Sharon, Y, Beer, S, Silva, J. 2009. Photosynthetic responses of
Halophila stipulacea to a light gradient: I – In situ energy partitioning of non-photochemical
quenching. Aquatic Biology 7: 143-152. IF=1.380.
268. Saavedra, M, Barr, Y, Pousão-Ferreira, P, Helland, S, Yúfera, M, Dinis, MT, Conceicão, LEC. 2009.
Supplementation of tryptophan and lysine in Diplodus sargus larval diet: effects on growth and
skeletal deformities. Aquaculture Research 40: 1191-1201. IF=1.099.
269. Saavedra, M, Pousão-Ferreira, P, Yúfera, M, Dinis, MT, Conceição, LEC. 2009. A balanced amino
acid diet improves Diplodus sargus larval quality and reduces nitrogen excretion. Aquaculture
Nutrition 15: 517-524. IF=1.482.
270. Sabour, B, Loudiki, M, Vasconcelos, V. 2009. Growth responses of Microcystis ichthyoblabe
Kützing and Anabaena aphanizomenoides Forti (Blue-green algae) under different nitrogen and
phosphorus conditions. Chemistry and Ecology 25(5): 337-344. IF=0.634.
271. Sabour, B, Sbiyyaa, B, Loudiki, M, Oudra, B, Belkoura, M, Vasconcelos, V. 2009. Effect of light and
temperature on the population dynamics of two toxic bloom forming Cyanobacteria –
Microcystis ichthyoblabe and Anabaena aphanizomenoides. Chemistry and Ecology 25(4): 277289. IF=0.634.
272. Saker, M, Moreira, C, Martins, J, Neilan, B, Vasconcelos, V. 2009. DNA profiling of complex
bacterial populations: toxic cyanobacterial blooms. Applied Microbiology and Biotechnology 85:
237-252. IF=2.896.
273. Santos, M, Marcos, R, Santos, N, Malhão, F, Monteiro, RAF, Rocha, E. 2009. An unbiased
stereological study on the sub-populations of rat liver macrophages and on their numerical
relation with the hepatocytes and stellate cells. Journal of Anatomy 214: 744-751. IF=2.134.
274. Santos, MJ, Saraiva, A, Cruz, C, Eiras, JC, Hermida, M, Ventura, C, Soares, JP. 2009. Use of
parasites as biological tags in stock identification of black scabbard fish, Aphanopus carbo Lowe,
1839 (Osteichthyes, Trichiuridae) from Portuguese waters. Scientia Marina 73(S2): 55-62.
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276. Saqrane, S, El Ghazali, I, Oudra, B, Bouarabab, L, Dekayir, S, Mandi, L, Ouazzani, N, Vasconcelos,
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microcystins. Toxicon 53(1): 9-14. IF=2.128.
277. Saraiva, A, Boane, C, Cruz, C. 2009. Effects of gryporhynchid metacestodes (Cestoda:
Cyclophyllidae) in common carp (Cyprinus carpio) from Mozambique. Bulletin of the European
Association of Fish Pathologists 29(4): 139-143. IF=0.513.
278. Saraiva, A, Ramos, MF, Barandela, T, Sousa, JA, Rodrigues, PN. 2009. Cryptosporidium sp.
(Apicomplexa) from cultured turbot Psetta maxima. Bulletin of the European Association of Fish
Pathologists 29(1): 34-36. IF=0.513.
279. Saraiva, JL, Barata, EN, Canario, AVM, Oliveira, RF. 2009. The effect of nest aggregation on the
reproductive behaviour of the peacock blenny Salaria pavo. Journal of Fish Biology 74(4): 754762. IF= 1.226.
280. Sarrico, CS, Teixeira, PN, Rosa, MJ, Cardoso, MF. Subject mix and productivity in Portuguese
universities. European Journal of Operational Research 197: 287-295. IF=2.093.
281. Sharon, Y, Silva, J, Santos, R, Runcie, J, Chernihovsky, M, Beer, S. 2009. Photosynthetic responses
of Halophila stipulacea to a light gradient: II – plastic acclimations following transplantations.
Aquatic Biology 7: 153-157. IF=1.380.
282. Silva, DM, Samhan-Arias, AK, Garcia-Bereguiain, MA, Tiago, T, Gutierrez-Merino, C. 2009. Major
plasma membrane-associated redox centres co-localize with L-type calcium channels in neuronal
lipid rafts microdomains. Free Radical Research 43: 69-69. IF=2.215.
283. Silva, J, Sharon, Y, Santos, R, Beer, S. 2009. Measuring seagrass photosynthesis: Methods and
applications. Aquatic Biology 7: 127-141. IF=1.380.
284. Silva, JMG, Espe, M, Conceicão, LEC, Dias, J, Valente, LMP. 2009. Senegalese sole juveniles (Solea
senegalensis Kaup, 1858) grow equally well on diets devoid of fish meal provided the dietary
amino acids are balanced. Aquaculture 296: 309-317. IF=1.925.
285. Silva, K, Almada, VC, Vieira, MN, Monteiro, NM. 2009. Female reproductive tactics in a sex role
reverser pipefish: screening for quality and number. Behavioural Ecology 20: 768-772. IF=2.981.
286. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2009. Can the limited marsupium space be a
limiting factor for females? Insights from the black-striped pipefish, Syngnathus abaster. Journal
of Animal Ecology 77(2): 390-394. IF=3.714.
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Research and Cell Motility 30: 29-39. IF=1.657.
288. Silva, P, Valente, LMP, Malhão, F, Galante, MH, Monteiro, RAF, Rocha, E. 2009. Fibre types in the
skeletal muscle of blackspot seabream (Pagellus bagaraveo Brunnich) juveniles: a histochemical
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289. Silva, P, Valente, LMP, Olmedo, M, Galante, MH, Monteiro, RAF, Rocha, E. 2009. Hyperplastic and
hypertrophic growth of lateral muscle in the fish blackspot seabream, Pagellus bogaraveo, from
hatching to juvenile. Journal of Fish Biology 74: 37-53. IF=1.226.
290. Silva, SR, Cunha, S, Matos, A, Cruz, N. 2009. Shallow water surveying using experimental
interferometric synthetic aperture sonar. Marine Technology Society Journal 43: 50-63. IF=0.514.
291. Soares, J, Coimbra, AM, Reis-Henriques, MA, Monteiro, NM, Vieira, MN, Oliveira, JMA, GuedesDias, P, Fontainhas-Fernandes, A, Parra, SS, Carvalho, AP, Castro, LF, Santos, MM. 2009.
292. Solé, M, Kopecka, J, Blasco, J. 2009. Pollution biomarkers in two estuarine invertebrates Nereis
diversicolor and Scrobicularia plana from the Cano Sancti-Petri Marsh in the SW Spain.
Environment International 35: 523-531. IF=4.786.
293. Sousa, R, Gutiérrez, JL, Aldridge DC. 2009. Non-indigenous invasive bivalves as ecosystem
engineers. Biological Invasions 11: 2367-2385. IF=3.074.
294. Stein, R, Hefter, J, Grützner, J, Voelker, A, Naafs, BDA. 2009. Variability of surface-water
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295. Sykes, AV, Almansa, E, Lorenzo, A, Andrade, JP. 2009. Lipid characterization of both wild and
cultured eggs of cuttlefish (Sepia officinalis) throughout the embryonic development.
296. Sykes, AV, Oliveira, AR, Domingues, PM, Cardoso, CM, Andrade, JP, Nunes, ML. 2009. Assessment
of European cuttlefish (Sepia officinalis, L.) nutritional value and freshness under ice storage
using a developed Quality Index Method (QIM) and biochemical methods. LWT - Food Science
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297. Thomsen, MS, Wernberg, T, Tuya, F, Silliman, BR. 2009. Evidence for impacts of nonindigenous
macroalgae: a meta-analysis of experimental field studies. Journal of Phycology 45: 812-819.
IF=2.270.
298. Tiago, T, da Silva, DM, Samhan-Arias, AK, et al. 2009. Actin cytoskeleton disruption in an early
event upon exposure to cerebellar granule neurons to SIN-1-induced oxidative stress. Free
Radical Research 43(S1): 70. IF=2.215.
299. Tim-Tim, AL, Morgado, F, Moreira, S, Rangel, R, Nogueira, AJ, Soares, AM, Guilhermino, L. 2009.
Cholinesterase and glutathione S-transferase activities of three mollusk species from the NW
Portuguese coast in relation to the ‘Prestige’ oil spill. Chemosphere 77: 1465-1475. IF=3.253.
300. Tittley, I, Neto, AI, Parente, MI. 2009. The marine algal (seaweed) flora of the Azores: additions
and amendments 3. Botanica Marina 52: 7-14. IF=1.090.
301. Trubiroha, A, Wuertz, S, Frank, SN, Sures, B, Kloas, W. 2009. Expression of gonadotropin subunits
in roach (Rutilus rutilus, Cyprinidae) infected with plerocercoids of the tapeworm Ligula
intestinalis (Cestoda: Bothriocephalidea). International Journal for Parasitology 39: 1465-1473.
IF=3.819.
302. Tsui, TKN, Hung, CCY, Nawata, MC, Wilson, JM, Wright, PA, Wood, CM. 2009. Ammonia transport
in cultured gill epithelium of freshwater rainbow trout: the importance of Rhesus glycoproteins
+
+
and the presence of an apical Na /NH4 exchange complex. Journal of Experimental Biology 212:
878-892. IF=2.722.
303. Tuya, F, Haroun, RJ. 2009. Phytogeography of Lusitanian Macaronesia: biogeographic affinities in
species richness and assemblage composition. European Journal of Phycology 44: 405-413.
IF=1.556.
304. Tuya, F, Wernberg, T, Thomsen, MS. 2009. Colonization of gastropods on subtidal reefs depends
on density in adjacent habitats, not disturbance regime or latitude. Journal of Molluscan Studies
75: 27-33. IF=1.074.
305. Tuya, F, Wernberg, T, Thomsen, MS. 2009. Habitat structure affect abundances of labrid fishes
across temperate reefs in south-western Australia. Environmental Biology of Fishes 86: 311-319.
IF=1.155.
306. Tzedakis, PC, Pälike, H, Roucoux, KH, de Abreu, L. 2009. Atmospheric methane, southern
European vegetation and low-mid latitude links on orbital and millennial timescales. Earth and
Planetary Science Letters 27: 307-317. IF=4.062. (*)
307. Urbatzka, R, Waterman, B, Lutz, I, Kloas, W. 2009. Exposure of Xenopus laevis tadpoles to
finasteride, an inhibitor of 5-alpha reductase activity, impairs spermatogenesis and alters
hypophyseal feedback mechanisms. Journal of Molecular Endocrinology 43: 209-219. IF=3.221.
308. Van de Vliet, MS, Diekmann, OE, Serrão, EA, Beja, P. 2009. Isolation of highly polymorphic
microsatellite loci for a species with a large genome size: Sharp-ribbed Salamander (Pleurodeles
waltl). Molecular Ecology Resources 9: 425-428. IF=1.251.
309. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2009. Development and characterization of
highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripes.
Conservation Genetics 10: 993-996. IF=1.849.
310. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2009. Highly polymorphic microsatellite loci
for the Parsley frog (Pelodytes punctatus): characterization and testing for cross-species
amplification. Conservation Genetics 10: 665-668. IF=1.849.
311. Van de Vliet, MS, Diekmann, OE, Serrão, ETA. 2009. Highly polymorphic microsatellite markers
for the short-snouted seahorse (Hippocampus hippocampus), including markers from a closely
related species the long-snouted seahorse (Hippocampus guttulatus). Conservation Genetics
Resources 1: 93-96.
312. van der Veer, HW, Cardoso, JFMF, Kooijman, SALM. 2009. From Static Energy Budgets (SEB) to
Dynamic Energy Budgets (DEB), linking physiology and ecology, illustrated for plaice Pleuronectes
platessa (L.). Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology
153A: S148. IF=2.196.
313. van der Veer, HW, Cardoso, JFMF, Peck, MA, Kooijman, SALM. 2009. Physiological performance
of plaice Pleuronectes platessa (L.): A comparison of static and dynamic energy budgets. Journal
of Sea Research 62: 83-92. IF=1.803.
314. Vasconcelos, P, Gaspar, MB, Castro, M, Nunes, ML. 2009. Influence of growth and reproductive
cycle on the meat yield and proximate composition of Hexaplex trunculus (Gastropoda:
Muricidae). Journal of the Marine Biological Association of the United Kingdom 89: 1223-1231.
IF=0.995.
315. Veiga, P, Machado, D, Almeida, C, Bentes, L, Monteiro, P, Oliveira, F, Ruano, M, Erzini, K,
Gonçalves, JMS. 2009. Weight-length relationships for 54 species of the Arade estuary, south
Portugal. Journal of Applied Ichthyology 25: 493-496. IF=1.121.
316. Veiga, P, Rubal, M, Besteiro, C. 2009. Shallow sublittoral meiofauna communities and sediment
polycyclic aromatic hydrocarbons (PAHs) content on the Galician coast (NW Spain), six months
after the Prestige oil spill. Marine Pollution Bulletin 58: 581-588. IF=2.630.
317. Velez, Z, Hubbard, PC, Barata, EN, Canario, AVM. 2009. Adaptation to reduced salinity affects the
2+
+
olfactory sensitivity of Senegalese sole (Solea senegalensis Kaup 1858) to Ca and Na but not
amino acids. Journal of Experimental Biology 212(16): 2532-2540. IF=2.722.
318. Velez, Z, Hubbard, PC, Welham, KJ, Barata, EN, Hardege, JD, Canário, AVM. 2009. Functional
asymmetry in the olfactory system of a flatfish, the Senegalese sole (Solea senegalensis).
Comparative Biochemistry and Physiology A - Molecular and Integrative Physiology 153(2): S90.
IF=2.196.
319. Velez, Z, Hubbard, PC, Welham, K, Hardege, JD, Barata, EN, Canario, AVM. 2009. Identification,
release and olfactory detection of bile salts in the intestinal fluid of the Senegalese sole (Solea
senegalensis), Journal of Comparative Physiology A - Neuroethology Sensory Neural and
Behavioral Physiology 195(7): 691-698. IF=2.196.
320. Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML,
Simes, DC. 2009. Gla-rich protein (GRP) is a novel vitamin K dependent protein present in serum
and accumulated at sites of pathological calcifications. American Journal of Pathology 175(6):
2288-2298. IF=5.673.
321. Vieira, LR, Gravato, C, Soares, AMVM, Morgado, F, Guilhermino, L. 2009. Acute effects of copper
and mercury on the estuarine fish Pomatoschistus microps: linking biomarkers to behaviour.
Chemosphere 76: 1416-1427. IF=3.253.
322. Villacorta, C, Cánovas, F, Oromí, P, Juan, C. 2009. Isolation and characterization of microsatellite
loci for the cavehopper Palmorchestia hypogaea (Amphipoda: Talitridae). Conservation Genetics
Resources 1: 401-404.
323. Villanueva, RD, Hilliou, L, Sousa-Pinto, I. 2009. Postharvest culture in the dark: an eco-friendly
alternative to alkali treatment for enhancing the gel quality of κ/ι-hybrid carrageenan from
Chondrus crispus (Gigartinales, Rhodophyta). Bioresource Technology 100: 2633-2638. IF=4.253.
324. Voelker, A, Rodrigues, T, Stein, R, Billups, K, Oppo, D, McManus, J, Hefter, J, Grimalt, JO. 2009.
Variations in mid-latitude North Atlantic surface water properties during the mid-Brunhes: Does
Marine Isotope Stage 11 stand out?. Climate of the Past 5(3): 1553-1607. IF=3.826.
325. Voelker, AHL, de Abreu, L, Schönfeld, J, Erlenkeuser, H, Abrantes, F. 2009. Hydrographic
conditions along the Western Iberian Margin during Marine Isotope Stage 2. Geochemistry,
326. Waeschenbach, A, Cox, CJ, Littlewood, DTJ, Porter, JS, Taylor, PD. 2009. First molecular estimate
of cyclostome bryozoan phylogeny confirms extensive homoplasy among skeletal characters
used in traditional taxonomy. Molecular Phylogenetics and Evolution 52: 241-251. IF=3.556.
327. Wallenstein, FM, Couto, RP, Amaral, AS, Wilkinson, M, Neto, AI, Rodrigues, AS. 2009. Baseline
metal concentrations in marine algae from São Miguel (Azores) under different ecological
conditions - urban proximity and shallow water hydrothermal activity. Marine Pollution Bulletin
58: 424-455. IF=2.630.
328. Wallenstein, FM, Terra, MR, Pombo, J, Neto, AI. 2009. Macroalgal turfs in the Azores. Marine
Ecology - An Evolutionary Prespective 30: 113-117. IF=1.558.
329. Wallenstein, FM, Torrão, DF, Neto, AI, Wilkinson, M, Rodrigues, A. 2009. Accumulation of metals
in Fucus spiralis subject to increased temperature and acidity. Phycologia 48(4): 92-92. IF=1.218.
330. Wallenstein, FM, Torrão, DF, Neto, AI, Wilkinson, M, Rodrigues, A. 2009. Effect of exposure time
on the bioaccumulation of Cd, Mg, Mn and Zn in Cystoseira abies-marina samples subject to
shallow water hydrothermal activity in São Miguel (Azores). Marine Ecology - an Evolutionary
Prespective 30: 118-122. IF=1.558.
331. Wirtz, P, Anker, A. 2009. Range extension for Elysia timida (Opisthobranchia, Sacoglossa) to São
Tomé Island (eastern central Atlantic), with a film showing the curious locomotion of the species.
Marine Biodiversity Records 2: e 144.
332. Wirtz, P, Canning-Clode, J. 2009. The invasive bryozoan Zoobotryon verticillatum has arrived at
Madeira Island. Aquatic Invasions 4: 669-670.
333. Wirtz, P, de Melo, G, Grave, S. 2009. Decapoda from Actiniaria, Gorgoniaria, Antipatharia and
Echinodermata at the coasts of Espirito Santo, Brazil. Marine Biodiversity Records 2: e 162.
(*) Protocol of collaboration with LNEG.
PUBLICATIONS IN INTERNATIONAL PEER REVIEWED PROCEEDINGS
1.
Aragão, C, Weber, RA, Costas, B, Dinis, MT, Conceição, LEC. 2009. Effects of ammonia exposure in
Senegalese sole (Solea senegalensis) larvae. Proceedings of the Larvi’09 - Fish & Shellfish
Larviculture Symposium. Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). European
Aquaculture Society, Special Publication nº38: 14-17.
2.
Bio, A, Couto, A, Costa, R, Prestes, A, Vieira, N, Valente, A, Azevedo, J. 2009. Biomanipulation of
Furnas Lake, Azores: effects of repeated fish removal. Proceedings of the International
Association of Theoretical and Applied Limnology - Verhandlungen Internationale Vereinigung für
Theoretische und Angewandte Limnologie 30(8): 1207-1209.
3.
Borges, M-T, Santos, I, Restivo, MT, Mendes, JG, Ribeiro, H, Pereira, CM. 2009. Solving the
challenge of hyperintensive aquaculture: getting the maximum monitoring output from the
minimum water input. Proceedings of IRF2009 - 3rd International Conference on Integrity,
Reliability & Failure, 20-24 July, Porto, Portugal. Silva Gomes, JF, Meguid, SA (Eds), Chapter II:
Instrumentation and Engineering Measurement. Faculty of Engineering, University of Porto,
INEGI Editions, 6 p, ISBN 978-972-8826-22-2, ISBN: 978-972-8826-21-5 (CD-ROM).
4.
Couto, MNF, Vasconcelos, MTSD. 2009. Phytoremediation of hydrocarbons from a refinery’s soil the role of rhizosphere. Proceedings of the International Symposium on Contaminated Soils and
Sediments, pp. 289-295.
5.
Cunha, ME, Quental Ferreira, H, Barradas, A, Pousão-Ferreira, P, Cabrita, E, Engrola, S. 2009.
Growth and development of Dusky grouper Epinephelus marginatus larvae in mesocosm of semiintensive technology. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium.
Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). European Aquaculture Society, Special
Publication nº38: 52-55.
6.
Engrola, S, Dinis, MT, Conceição, LEC. 2009. Senegalese sole is able to adapt protein metabolism
when co-fed with Artemia replacement. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture
Symposium. Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). European Aquaculture
7.
Fernandes, C, Fontaínhas-Fernandes, A, Coimbra, A, Saavedra, MJ, Salgado, MA. 2009.
Biochemical blood parameters: are they effective biomarkers of chronic toxicity?. Proceedings of
the SECOTOX Conference and CEMEPE - International Conference on Environmental
Management, Engineering, Planning and Economics, 21-26 June, Mykonos, Greece. Kungolos, A,
Aravossis, K, Karagiannidis, A, Samaras, P (Eds), Vol. I, pp. 191-196. ISBN 978-960-6865-09-1.
8.
Mesquita, A, Avilez-Valente, P. 2009. Numerical depth inversion of the entrance of Leixões
harbor. Proceedings of the Twelfth International Conference on Civil, Structural and
Environmental Engineering Computing - CC2009. Topping, BHV, Costa Neves, LF, Barros, RC (Eds),
1-4 September, Funchal, Portugal, Paper 248, 16 pp., CD-ROM, Civil-Comp Press, Stirlingshire, UK.
ISBN 978-1-905088-32-4.
9.
Morais, S, Alves Martins, D, Castanheira, F, Mendes, A, Coutinho, J, Bandarra, NM, PousãoFerreira, P, Conceição, LEC. 2009. A new method for the study of essential fatty acid
requirements in fish larvae. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium.
Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). European Aquaculture Society, Special
Publication nº38: 279-281.
10. Moreira, C, Lopes, VR, Vasconcelos, V. 2009. Diversidade e análise da toxicidade de
cianobactérias no ecossistema estuarino do rio Minho. Proceedings of the 4th Iberan Symposium
of the Hydrographic Basin of River Minho, pp. 147-151. ISBN 978-989-96062-0-3.
11. Oliva-Teles, A. 2009. Nutritional strategies for the health and welfare of aquaculture fish.
Proceedings of the 3rd International Symposium of Fish Nutrition and Health, 4-6 November,
Botucatu, Brasil, pp. 80-99.
12. Pinto, W, Figueira, L, Rodrigues, V, Dinis, MT, Aragão, C. 2009. The importance of aromatic amino
acids during fish metamorphosis. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture
Symposium. Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P. (Eds). European Aquaculture
13. Ribeiro, R, Hubbert, F, Rojas-García, C, Rønnestad, I, Engrola, S, Dinis, MT, Power, D. 2009. Cofeeding in Senegalese sole at mouth opening: Consequences on digestive physiology.
Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium. Hendry, CI, Van Stappen, G,
Wille, M, Sorgeloos, P. (Eds). European Aquaculture Society, Special Publication nº38: 362-365.
OTHER INTERNATIONAL PUBLICATIONS
1.
Cabral, JP. 2009. Gonçalo Sampaio y el estudio moderno da la flora ibérica. Análisis de
manuscritos epistolares. Boletín de la Real Sociedad Española de Historia Natural (Sección
Biológica) 103(1-4): 9-26.
2.
Oropesa, AL, Soler, F, Zalba, J, Gravato, C, Guilhermino, L. 2009. Defensas antioxidantes de origen
enzimático en cigüeña branca (Ciconia ciconia): aplicación para biomonitorización de la
contaminación por agents que originan fenómenos de estrés oxidativo. Revista de Toxicología 26:
67.
3.
Pereira, R. 2009. Seaweeds under the spotlight. Alga - Bulletin of the Spanish Phycological Society
41: 13-15.
4.
Veiga, P, Rubal, M, Besteiro, C. 2009. Primera cita de Orzeliscus belopus Du Bois-Reymond
Marcus, 1952 para la Península Ibérica, con notas sobre otros tardígrados encontrados en las rías
de O Barqueiro y Foz (Galicia, NO Península Ibérica). Boletín de la Real Sociedad Española de
Historia Natural 103: 59-65.
OTHER NATIONAL PUBLICATIONS
1.
B.
Marques, CL, Rafael, MS, Tiago, DM, Cancela, ML, Laizé, V. 2009. Desenvolvimento de sistemas
celulares de peixe adequados ao estudo da mineralização in vitro. Canal BQ 6: 15-20.
ADVANCED TRAINING
PhD THESES
1.
Alquicira, Edgar Francisco Rosas. 2009. Filogeografia de algas Corallinales (Rhodophyta) das
Califórnias. PhD thesis in Marine Botany, University of Azores. Supervisor: Ana Isabel Neto, Cosupervisor: Rafael Riosmena Rodríguez (University of Baja Califórnia Sur, Mexico). (Thesis
submitted in 2009, defense in 2010)
2.
Amaral, Ana Margarida. 2009. Aquaculture impact of clam Ruditapes decussatus in the Ria
Formosa: effects on the ecosystems and species physiology. PhD thesis, University of Santiago de
Compostela, Spain. Supervisor: Luís Chicharo, Co-supervisor Marisa José Reiriz (CSIC, Vigo, Spain).
3.
Brito, Pedro. 2009. Impactos da elevação do nível médio do mar em ambientes costeiros: O caso
do Estuário do Sado. PhD thesis in Geology, University of Lisbon. Supervisor: F Abrantes.
4.
Campos, Joana Costa Vilhena de Bessa. 2009. The eco-geography of the brown shrimp Crangon
crangon in Europe. PhD thesis, Vrije Universiteit Amsterdam, The Netherlands. Supervisor: SALM
Kooijman, Co-supervisor: Henk van der Veer (NIOZ - Royal Netherlands Institute for Sea
Research, The Netherlands).
5.
Carvalho, Liliana. 2009. Metodologias para a avaliação integrada dos impactos cumulativos em
Aquatic Sciences, ICBAS, University of Porto. Supervisor: Adriano Bordalo e Sá, Co-Supervisors:
Rui Cortes (UTAD) and Joaquim Poças Martins (Faculty of Engineering, University of Porto).
6.
Casal, Graça. 2009. Microsporidioses e Mixosporidioses da Ictiofauna Portuguesa e Brasileira:
caracterização ultrastrutural e filogenética. PhD thesis in Biomedical Sciences, ICBAS, University
of Porto. Supervisors: Jorge Eiras and Maria Leonor Teles Grilo.
7.
Cisneros, Karla León. 2009. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais.
PhD thesis, University of Azores. Supervisor: Ana Isabel Neto, Co-supervisor: Rafael Riosmena
Rodríguez (University of Baja Califórnia Sur, Mexico). (Thesis submitted in 2009, defense in 2010)
8.
Kolmakov, Nikolay. 2009. Pheromone olfactory receptors in fish: isolation and functional
characterization. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Adelino
VM Canario, Co-supervisor: João Coimbra.
9.
Laporte, Jerome. 2009. Nutritional evaluation of animal by-products for the partial replacement
of fish meal in diets for gilthead sea bream Sparus aurata. PhD thesis, School of Biological
Sciences, Faculty of Sciences, University of Plymouth, UK. Supervisor: S Davies (University of
Plymouth), Co-supervisor: António Gouveia.
10. Leitão, Francisco Miguel de Sousa. 2009. Algarve Artificial reefs colonization processes and fish
trophic ecology: implications for the local near shore fisheries. PhD thesis, University of Algarve.
Supervisor: Karim Erzini, Co-supervisor: MN dos Santos (IPIMAR).
11. Lima, Inês Marrazes. 2009. Ecotoxicological effects of petrochemical products on natural
populations of Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal.
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Lúcia Guilhermino, Cosupervisor: Amadeu MVM Soares (CESAM, University of Aveiro). (Thesis submitted in 2009,
defense in January 2010)
12. Marçalo, Ana Luísa Barreto. 2009. Sardine (Sardina pilchardus) delayed mortality associated with
purse seine slipping: contributing stressors and responses. PhD thesis, University of Algarve.
Supervisor: Karim Erzini, Co-supervisor: Yorgos Stratoudakis (IPIMAR).
13. Martins, Rute. 2009. Is DAX-1 a sex-determining gene in fish? PhD thesis in Aquatic Sciences,
ICBAS, University of Porto. Supervisor: Adelino VM Canario, Co-supervisor: João Coimbra.
14. Moreira da Silva, Joana. 2009. Ammonia tolerance in the teleost fish Misgurnus anguillicaudatus.
Surviving high ammonia and aerial exposure. PhD thesis in Aquatic Sciences, ICBAS, University of
Porto. Supervisor: João Coimbra, Co-supervisor: Jonathan Wilson.
15. Moreira de Sousa, Joaquim João. 2009. Potential of integrating psi methodologies in the
detection of surface deformation. PhD thesis in Surveying Engineering, Faculty of Sciences,
University of Porto. Supervisor: Luísa Bastos, Co-supervisor: Ramon Hanssen (TUD, Delft). (Thesis
16. Ramos, A. 2009. Molecular basis of isoprenoid biosynthesis in Dunaliella salina. PhD thesis,
University of Algarve. Supervisor: João Varela, Co-Supervisor: Bertram Brenig (Göttingen,
Germany).
17. Rodrigues, Teresa. 2009. Reconstrução das condições climáticas na Margem Portuguesa durante
o Holocénico - Analogias com o Estádio 11. PhD thesis, Cataluña Tecnical Universitat, Spain.
Supervisor: Fátima Abrantes.
18. Saraiva, João. 2009. Inter-populational variation of reproductive behaviour in Salaria pavo. PhD
thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Rui Oliveira (ISPA), Cosupervisors: Adelino VM Canario and João Coimbra.
19. Seixas, Pedro. 2009. Composición bioquímica y crecimento de paralarvas de pulpo (Octopus
vulgaris Cuvier, 1797), alimentadas com juveniles de Artemia enriquecidos com microalgas y
otros suplementos nutricionales. PhD in Aquatic Sciences, ICBAS, University of Porto. Supervisor:
Manuel Rey Mèndez (University of Santiago Compostela, Spain), Co-supervisor: Ana Otero
(University of Santiago Compostela, Spain), Luísa Valente.
20. Silva, Amélia Cláudia Figueiredo. 2009. Nutritional regulation of lipid deposition in blackspot
seabream (Pagellus bogaraveo). PhD in Aquatic Sciences, ICBAS, University of Porto. Supervisor:
Luísa Valente, Co-supervisor: Geneviéve Corraze (Station de Recherches en Hydrobiologie, INRA,
France). (Submitted in 2009, defense in January 2010).
21. Silva, Joana Margarida Guimarães. 2009. Effectiveness of alternative protein sources in meeting
the true amino acid requirements of Senegalese sole. PhD in Aquatic Sciences, ICBAS, University
of Porto. Supervisor: Luísa Valente, Co-supervisors: Marite Espe (NIFES, Norway), Luís Conceição.
22. Silva, Paula Cristina Videira. 2009. Differentiation, development and growth of the blackspot
seabream (Pagellus bogaraveo) muscle. PhD thesis in Aquatic Sciences, ICBAS, University of
Porto. Supervisor: Eduardo Rocha, Co-supervisor: Luísa Valente. (Submitted in 2009, defense in
January 2010).
23. Velez, Zélia. 2009. Neural mechanisms of olfactory discrimination in the Senagalese sole, Solea
senegalensis Kaup, 1858. PhD thesis, University of Évora. Supervisors: Eduardo N Barata and
Peter C Hubbard, Co-supervisor: Adelino VM Canario.
24. Vieira, Luís Miguel Russo. 2009. Methodologies for a sustainable development of estuarine
ecosystems. PhD thesis in Biology, University of Aveiro. Supervisor: Lúcia Guilhermino, Cosupervisor: Fernando Morgado (CESAM, University of Aveiro).
MASTER THESES
1.
Álvaro, Nuno Miguel Vaz. 2009. Proposta de plano de monitorização para a área protegida para a
gestão de habitats ou espécies do Ilhéu de Vila Franca do Campo. Master thesis, University of
Azores. Supervisor: Ana Isabel Neto, Co-supervisor: Fátima Alves (University of Aveiro). (Thesis
2.
Araújo, Amândio. 2009. Presença de Legionella sp. em redes de distribuição hospitalar e sua
Porto), Co-supervisor: José Américo Sousa.
3.
Barroso, Maria de Fátima Saavedra. 2009. Efeitos ecotoxicológicos de pesticidas e factores
abióticos em Daphnia magna. Master thesis in Environmental Contamination and Toxicology,
Faculty of Sciences and ICBAS, University of Porto. Supervisor: Lúcia Guilhermino, Co-supervisor:
Laura Guimarães.
4.
Benitez, Sonia Rodríguez. 2009. Consecuencias de la diversidad en la productividad de las
comunidades de las macroalgas. Master thesis in Coastal Management, University Las Palmas
Gran Canaria. Supervisor: Francisco Arenas.
5.
Borges, Pedro Alexandre Coelho. 2009. Optimização do nível lipídico em dietas para juvenis de
linguado. Master thesis in Quality Control - Water and Sediments, Faculty of Pharmacy,
University of Porto. Supervisor: Beatriz Oliveira (Faculty of Pharmacy, University of Porto), Cosupervisor: Luísa Valente.
6.
Cabrita, Carina Isabel. 2009. Functional characterisation of novel neuropeptides in vertebrates.
Master thesis in Biological Engeneering, University of Algarve. Supervisors: João Cardoso and
Deborah M Power.
7.
Campos do Mar, António. 2009. Caracterização endócrina da PTH-L e calcitonina no transporte
de cálcio e na secreção de bicarbonato no intestino da dourada (Sparus auratus). Master thesis in
Marine Biology, University of Algarve. Supervisors: Deborah M Power and Juan Fuentes.
8.
Carvalho, Edison. 2009. Disrupção da glândula tiróide pelo composto goitrogeno (PTU): estudo
de aproximação molecular e morfo-funcional da tiróide e pele de Xenopus laevis. Master thesis in
9.
Castanheira, Maria Filipa. 2009. Metabolic basis of growth variation in juvenile sole (Solea
senegalensis, Kaup 1858) 2009. Master thesis, Faculty of Sciences and Technology, University of
Algarve. Supervisors: Luís Conceição and Sofia Engrola.
10. Claro, Bruno. 2009. Caracterização sócio-económica do turista de observação de cetáceos do
Algarve e determinação do seu grau de satisfação e perfil ecológico. Master thesis in Marine
Biology, University of Algarve. Supervisors: Margarida Castro, Sara Magalhães (Mar Ilimitado,
Sagres).
11. Coelho, Daniela Filipa. 2009. Protozoários na avaliação de ETAR. Caracterização do tanque de
arejamento com base no estudo da comunidade de protozoários. Master thesis in Biology,
Faculty of Sciences, University of Porto. Supervisor: Maria Leonor Fidalgo, Co-supervisor: Ana
Nicolau (University of Minho).
12. Coelho, Nelson Alexandre. 2009. Análise e caracterização da expressão e papel funcional de
potenciais genes da família 2 GPCRs no protostómio Caenorhabditis elegans. Master thesis in
Biotechnology, University of Algarve. Supervisors: João Cardoso and Deborah M Power.
13. Costa, Joana Ferreira. 2009. Spiraling among the Fucus: the strange story of Fucus spiralis. Master
thesis in Marine Biology, University of Algarve. Co-supervisors: Ester Serrão, Gareth Pearson,
Jeanine Olsen (University of Groningen).
14. Cruzeiro, Catarina da Rocha. 2009. Efficacy of a phospholipase A2 (aPLA2) antibody on growth
performance of juvenile fish fed a practical diet. Master thesis, University of Trás-os-Montes and
Alto Douro (UTAD). Supervisor: Paulo Rema, Co-supervisor: Jorge Dias.
15. Damião, Vitor. 2009. Água da chuva, que utilização? Master thesis in Biology and Water Quality
Management, Faculty of Sciences, University of Porto. Supervisor: Maria Natividade Vieira.
16. Elias, Carmen Lopes. 2009. Utilização de diatomáceas na avaliação da qualidade do Rio Febros.
Master thesis in Biology and Water Quality Management, Faculty of Sciences, University of Porto.
Supervisor: Maria Natividade Vieira.
17. Encarnação, Ana Maria. 2009. Uso de métodos químicos na avaliação da toxicidade de
cianobactérias. Master thesis in Sciences and Environmental Technologies, Faculty of Sciences,
University of Porto. Supervisor: Vítor Vasconcelos.
18. Escórcio Pereira, Cláudia de Fátima. 2009. Suplementação de L-carnitina na dieta de trutas arcoíris (Oncorhynchus-mykiss): Estudo comparativo entre indivíduos diplóides e triplódes. Master
thesis in Sciences of the Sea - Sea Resources, ICBAS, University of Porto. Supervisor: José
Fernando Gonçalves, Co-supervisor: Rodrigo Ozório.
19. Freitas, Marisa. 2009. Monitorização de cianobactérias e cianotoxinas nas lagoas de Mira e Vela
comparando métodos moleculares, imunológicos e volumes de amostragem. Master thesis in
Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto.
Supervisor: Vítor Vasconcelos.
20. Gaspar Meirinho, Ana Isabel. 2009. Distribuição de alcatraz (Morus bassamus) na costa
continental portuguesa e sua relação com variáveis ambientais. Master thesis in Ecology,
Management and Modelling of Marine Resources, Faculty of Science and Technology, New
University of Lisbon. Supervisor: Ana Bio, Co-supervisor: João Gomes Ferreira (FCT/UNL).
21. Gomes, Joana Maria Braga Cavalheiro. 2009. Chemical characterization and influence of the
bottling process of a natural sparking mineral water. Master thesis, Faculty of Sciences,
University of Porto. Supervisor: Maria Clara Basto, Rui Santos (Lab. INETI, S. Mamede de Infesta).
22. Gomes, José Miguel. 2009. Efeito da inclusão de alimento vivo no desenvolvimento do estímulo
Paulo Rema, Co-supervisor: José Fernando Gonçalves.
23. Gomes, Marta Sofia. 2009. Determination of optimal larval rearing condition for broad noosed
pipefish (Syngnathus typhle, Linnaeus, 1758) (Pisces: Syngnathidae). Master thesis, University of
Algarve. Co-supervisors: José Pedro Andrade and Jorge Palma.
24. Gomes, Pedro. 2009. A importância da assinatura química elementar dos otólitos de
Spondyliosoma cantharus (n.v. choupa) na discriminação dos stocks pesqueiros e no estudo da
estrutura populacional. Master thesis in Marine Biology, Faculty of Marine and Environmental
Science, University of Algarve. Supervisor: Alberto Correia, Co-supervisor: Karim Erzini.
25. Gonçalves, R. 2009. Determination of dietary docosahexaenoic acid (DHA) requirements for
Senegalese sole (Solea senegalensis) post-larvae. Master thesis, Faculty of Sciences and
Technology, University of Algarve. Supervisors: Luís Conceição and Dulce Alves Martins.
26. Gouveia, Andreia. 2009. Conservação e educação ambiental no contexto de um Centro de
Monitorização e Interpretação Ambiental (CMIA de Vila do Conde). Master thesis in Biology,
Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.
27. Guedes, Joaquim Manuel Soares. 2009. Study of the indoor air quality in Porto City schools.
Master thesis, Department of Chemistry, Faculty of Sciences, University of Porto. Supervisor:
Maria Teresa Vasconcelos and Maria Clara Basto.
28. Inácio, MIM. 2009. Transporte de crustáceos vivos em ambiente refrigerado. Master thesis in
Marine Biology, University of Algarve. Supervisors: Margarida Castro, Aida Campos (IPIMAR).
29. Keller, T. 2009. Bacterial diversity in and cytotoxic potential of Pseudomonas spp. associated with
the freshwater sponge Ephydatia fluviatilis. Master thesis, Friedrich-Schiller University of Jena,
Germany. Supervisor: Jan Dirk van Elsas (University of Groningen, The Netherlands), Kirsten Küsel
(Friedrich-Schiller University of Jena, Germany), Co-supervisor: Rodrigo Costa.
30. Laverco, Paulo. 2009. Análise biológica de biofilmes em sistemas de transporte de águas de
University of Porto. Supervisor: Vítor Vasconcelos, Co-supervisor: Joaquim Poças Martins (Faculty
of Engineering, University of Porto).
31. Leitão, Alexandre Filipe. 2009. Caracterização dos genes Gase, GSase e GDH em Misgurnus
anguillicaudatus. Master thesis in Marine Biology, University of Aveiro. Supervisor: Jonathan
Wilson, Co-supervisor: Vítor M dos Santos Quintino (University of Aveiro)
32. Leocádio, Ana Maria. 2009. Economic and biologic evaluation of two métiers, creels and bottom
trawl, for catching Norway lobster off the Portuguese coast. International Master thesis in
Aquaculture and Fisheries, University of Algarve. Supervisors: Margarida Castro, David
Withmarsh (University of Portsmouth, UK).
33. Lima, Cristina. 2009. Efeitos de herbivoria na restauração de pradarias de ervas marinhas. Master
thesis in Marine Biology, University of Algarve. Co-supervisors: Ester Serrão, Jorge Gonçalves and
Alexandra Cunha.
34. Lopes, Evandro Pires. 2009. Filogeografia de Brachidontes puniceus (Gmelin, 1791) no
arquipélago de Cabo Verde. Master thesis in Marine Biology, University of Algarve. Supervisor:
Rita Castilho.
35. Luís, Gabriel da Silva Luís. 2009. Efeitos agudos do naftaleno no camarão Palaemon serratus:
mortalidade, resposta de biomarcadores e comportamento. Master thesis in Biology, Faculty of
Sciences, University of Porto. Supervisor: Carlos Gravato, Co-supervisor: Lúcia Guilhermino.
36. Magalhães, Américo. 2009. Análise da qualidade de ortofotomapas, modelo numérico altimétrico
e cartografia a grandes escalas. Master thesis in Geographic Engineering, Faculty of Sciences,
University of Porto. Supervisor: Luísa Bastos.
37. Maldonado, Emanuel. 2009. Development of a graphical user interface for a software used to
assess adaptive evolution in protein-coding genes. Master thesis in Computer Sciences, Faculty of
Sciences, University of Porto. Supervisor: Agostinho Antunes, Co-supervisor: Vítor Vasconcelos.
38. Marques, Maria Fernanda Ferreira. 2009. Respostas aguda e crónica de Daphnia magna a
cefalexina. Master thesis in Legal Medicine, ICBAS, University of Porto. Supervisor: Lúcia
Guilhermino.
39. Martins, Marta Lúcia Xavier. 2009. Eco-efficiency: development of assessment tools. Master
thesis, Faculty of Sciences, University of Porto. Supervisor: Maria Clara Basto, responsible from
Ecoinside: Joaquim Guedes.
40. Mateus, C. 2009. Remoção de nutrientes de águas eutrofizadas através de Salicornia ramossima.
Master Thesis, Marine Biology course - Ecology and Conservation specialty, University of Algarve.
Supervisor: Luís Chicharo and Rui Santos.
41. Mateus, Miguel. 2009. Characterization of the epibenthic macro-invertebrate community of the
Arade estuary. Master thesis, Faculty of Marine Sciences and Environment, University of Algarve.
Supervisors: Jorge MS Gonçalves and Karim Erzini.
42. Maximo, I. 2009. Avaliação da qualidade da água das ribeiras do Algarve: uso de
macroinvertebrados bentónicos como bioindicadores. Master thesis in Marine Biology - Ecology
and Conservation, University of Algarve. Supervisors: Luís Chicharo and Maria Alexandra
Chícharo.
43. Mazagão, Eduarda. 2009. Thyroid Axis disruption by goitrogens: a molecular and functional
approach. Integrated Master thesis in Biological Engineering, University of Algarve. Supervisors:
Deborah M Power and Patrícia Pinto.
44. Medeiros, João Vaz. 2009. Influência de marés na distribuição vertical de larvas de peixe de
recifes costeiros e escolha de substratos de assentamento no Parque Marinho Luíz Saldanha.
Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges and Ester
Serrão, Emanuel Gonçalves (ISPA).
45. Miodonski, Joana. 2009. Caracterização da Ictiofauna de intertidal rochoso na costa sul de
Portugal. Master thesis, Faculty of Marine Sciences and Environment, University of Algarve.
Supervisors: Jorge MS Gonçalves and Karim Erzini.
46. Morais, João. 2009. Avaliação do risco de ocorrência de cianotoxinas nos lagos do Parque da
Cidade, Porto. Master thesis in Biology and Management of Water Quality, Faculty of Sciences,
University of Porto. Supervisor: Vítor Vasconcelos.
47. Muntz, A. 2009. Linking different levels of biological organization after acute exposure of a widely
used pharmaceutical (diazepam) in Dicentrachus labrax). Master thesis in Écologie et
Catholique d’Ouest, Angers, France. Supervisor: Lúcia Guilhermino.
48. Oliveira, Patrícia Alexandra Correia. 2009. Ecotoxicological responses in wild populations of
mussels (Mytilus galloprovincialis) in relation to the exposure to selected polycyclic aromatic
hydrocarbons. Master thesis in Environmental Contamination and Toxicology, ICBAS and Faculty
of Sciences, University of Porto. Supervisor: Lúcia Guilhermino, Co-supervisor: Carlos Gravato.
49. Pedrosa, Katia Pinto. 2009. Efeito da inclusão na dieta de óleo e farinha de canabinoides na
ingestão alimentar de juvenis de rodovalho Scophtalmus maximus. Master thesis, University of
Trás-os-Montes and Alto Douro. Supervisor: Paulo Rema, Co-supervisor: Jorge Dias.
50. Pereira, Diana. 2009. Efeito da anosmia na endocrinologia e reprodução de machos de
Oreochromis mossambicus (Peters, 1852). Master thesis in Marine Biology, University of Algarve.
Supervisors: Teresa Modesto and Peter C Hubbard.
51. Pipa, Tânia. 2009. Discriminação do(s) stock(s) de Diplodus vulgaris (n.v. safia) na costa SW
Marine Biology, Faculty of Marine and Environmental Science, University of Algarve. Supervisor:
Alberto Correia, Co-supervisor: Karim Erzini.
52. Rebotim, Andreia Seia. 2009. Foraminíferos planctónicos como indicadores das massas de água a
norte e a sul da Frente/Corrente dos Açores: Evidências de dados de abundância e isótopos
estáveis. Master thesis in Sciences of the Sea - Sea Resources, ICBAS, University of Porto.
Supervisor: Antje Voelker.
53. Regueiras, Ana. 2009. Comparação de métodos de identificação e quantificação de
cianobactérias e suas toxinas na albufeira do Torrão (Rio Tâmega). Master thesis in
Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto.
Supervisor: Vítor Vasconcelos.
54. Reis, Rita. 2009. Pesquisa de bactérias patogénicas numa piscicultura do Norte de Portugal.
Master thesis in Biology and Management of Water, Faculty of Sciences, University of Porto.
Supervisor: José Américo Sousa.
55. Rocha, Filipa. 2009. Requisitos de ácido araquidónico em microdietas para larvas de Dourada
(Sparus aurata L., 1758). Master thesis, Faculty of Sciences and Technology, University of
Algarve. Supervisors: Luís Conceição and Dulce A Martins.
56. Rocha, Lígia Celeste. 2009. Toxicidade de um efluente têxtil e de auxiliares têxteis em peixe
zebra, Danio rerio. Master in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor:
Maria Leonor Fidalgo.
57. Sá, Eloise. 2009. Control of the eutrophication process in aquatic ecosystems. Master thesis in
Marine Biology - Ecology and Conservation, University of Algarve. Supervisors: Luís Chicharo and
Radhouan Ben-Hamadou.
58. Santinho, CIA. 2009. Padrões de distribuição da abundância larvar de crustáceos decápodes na
baía de Cascais. Master thesis in Marine Biology, University of Algarve. Supervisors: Margarida
Castro, Antonina dos Santos (IPIMAR).
59. Santos, C. 2009. Alterações histológicas nas brânquias e no rim do xarroco, Halobatrachus
didactylus (Bloch & Schneider, 1801), exposto a concentrações elevadas de amónia. Master
thesis, University of Algarve. Supervisors: Teresa Modesto and Maria Huertas.
60. Santos, Rui Pedro Peres. 2009. Identificação de efeitos de xenobioticos in vivo no
desenvolvimento do osso em peixe zebra (Danio rerio). Master thesis in Marine Biology,
University of Algarve. Supervisors: Maria Leonor Cancela, Paulo Jorge Gavaia and Anabela B
Brito.
61. Saraiva, Fernanda. 2009. Desenvolvimento de ferramentas didático-educativas na área da
divulgação ambiental no centro de Monitorização e Interpretação Ambiental de Vila do Conde.
Master thesis in Sciences and Environmental Technologies, Faculty of Sciences, University of
Porto. Supervisor: Vítor Vasconcelos.
62. Semedo, Miguel. 2009. Integrated biomarkers and interactive effects of copper (II) and cadmium
(II) with pyrene on antioxidant enzymes and lipid peroxidation of the mussel Mytilus
galloprovincialis. Master thesis in Biochemistry, ICBAS and Faculty of Sciences, University of
Porto. Supervisor: Lúcia Guilhermino.
63. Silva, Bruna Lopes. 2009. Studies of cultivation of red and brown algae in the laboratory, for
application in integrated multitrophic aquaculture systems. Master thesis, ICBAS, University of
Porto. Supervisor: Isabel Sousa-Pinto, Co-supervisor: Rui Pereira. (Thesis submitted in 2009,
defense in 2010)
64. Silva, João Cardeira. 2009. Efeito da dieta e da inanição na performance larvar e no
desenvolvimento de peixe-zebra (Danio rerio): caso específico do desenvolvimento esquelético.
Master thesis in Marine Biology, University of Algarve. Supervisors: Maria Leonor Cancela, Paulo
Jorge Gavaia and Anabela B Brito.
65. Silva, Lígia Maria Chaves. 2009. Qualidade biológica da água do rio Rabaçal. Master thesis in
Biology and Water Management, Faculty of Sciences, University of Porto. Supervisor: Maria
Natividade Vieira.
66. Silva, Marlene. 2009. Contribuição do estudo da microestrutura e microquímica de otólitos de
singnatídeos para a Ecologia das espécies, Syngnathus abaster e Nerophis lumbriciformes. Master
thesis in Applied Ecology, Faculty of Science, University of Porto. Supervisor: Alberto Correia, Cosupervisor: Nuno Monteiro.
67. Vicêncio, SC. 2009. Distribuição de macroalgas nativas e exóticas no substrato duro subtidal do
Porto de Sines. Master thesis in Ecology, Management and Modelling of Marine Resources, New
University of Lisbon. Supervisor: Rui Santos. (Submitted in 2009, defense in January 2010)
68. Vicente, AVH. 2009. Manutenção de lagostins (Nephrops norvegicus) vivos, a bordo de arrastões
comerciais. Master thesis in Marine Biology, University of Algarve. Supervisors: Margarida Castro,
Aida Campos (IPIMAR).
69. Vieira, Raquel Pinheiro. 2009. Estudo da dinâmica populacional de Grateloupia turuturu na costa
portuguesa. Master thesis in Costal Sciences, University of Aveiro. Supervisor: Isabel Sousa-Pinto.
70. Vieira, Pedro. 2009. Caracterização das espécies de Gorgónias (Cnidaria, Gorgonacea) da costa
Algarvia. Master thesis, Faculty of Marine Sciences and Environment, University of Algarve.
Supervisors: Jorge Manuel Gonçalves and Luís Fonseca.
C.
COMMUNICATIONS
COMMUNICATIONS IN INTERNATIONAL SCIENTIFIC MEETINGS
1.
Abecasis, D. 2009. Multispecies spatial dynamics under different protection levels: An evaluation
of the effects and optimal design of the Luiz Saldanha Marine Park (Portugal) - A PhD proposal.
AATAMS - 2nd Australian Acoustic Tracking and Monitoring System Workshop, 2-4 November,
Sydney, Australia.
2.
Abecasis, D, Almada, F, Cardigos, F, Gonçalves, JMS. 2009. Fish assemblages from the photic zone
of the Gorringe Seamount (northeaster Atlantic). 8th Indo Pacific Fish Conference and 2009 ASFB
Workshop & Conference, 31 May-5 June Fremantle, WA, Australia.
3.
Abrantes, F, Lopes, C, Matos, L. 2009. Médium Si and diatom preservation potential in sediments.
European Geosciences Union General Assembly, 20-24 April, Vienna, Austria. Geophysical
Research Abstracts, vol. 11, EGU2009-3689. (Oral communication)
4.
Abrantes, F, Rodrigues, T, Montanari, B, Santos, Voelker, A, Lopes, C. 2009. Precipitation maxima
and upwelling trends at the NAO Southern Pole during the Last Millenium. European Geosciences
Union General Assembly, 20-24 April, Vienna, Austria. Geophysical Research Abstracts, vol. 11,
EGU2009-3689. (Oral communication)
5.
Abreu, MH, Korbee, N, Pereira, R, Sousa-Pinto, I, Gil, C, Figueroa, F. 2009. Mycosporine-like
aminoacids (MAAs) from seaweeds: Relation with nitrogen availability and potential application
as photoprotector and antioxidant compounds. 6th European Conference on Marine Natural
Products, 19-23 July, Porto, Portugal.
6.
Abreu, MH, Pereira, R, Sousa-Pinto, I. 2009. Integrated Multi-Trophic Aquaculture in Portugal:
Experiments with Gracilaria vermiculophylla (Ohmi) Papenfuss at a sole and turbot commercial
aquaculture. IMMR’09, International Meeting on Marine Resources, 16-18 November, Peniche,
Portugal.
7.
Afonso, R, Leite, RB, Cancela, ML. 2009. Localization and characterization of chitin present in the
marine parasite Perkinsus olseni. XX Molecular Parasitology Meeting, 13-17 September, Woods
Hole, USA.
8.
Agüero Chapin, G, González-Díaz, H, de La Riva, GA, Pérez-Machado, G, Antunes, A. 2009. QSAR
study to polymeric sequences embedded in a novel 2D lattice Session Omica de Plantas. XIII
Congreso Nacional de Bioquímica y Biología Molecular de Plantas, 9-13 November, Guanajuato,
Mexico.
9.
Aires, T, Marba, N, Serrao, EA, Duarte, CM, Arnaud-Haond, S. 2009. Biotic interactions and the
success of invasive species: the case of the bacterial flora of Caulerpa taxifolia. DIVERSITAS OSC2,
Biodiversity and Society: Understanding Connections, Adapting to change, 13-16 October, Cape
Town, South Africa.
10. Alberto, F, Raimondi, P, Reed, DC, Coelho, NC, Leblois, R, Whitmer, A, Serrão, E. 2009. Habitat
continuity and geographic distance predict population genetic differentiation in giant kelp.
Australian Marine Science Association conference: Marine connectivity, 5-9 July, Adelaide,
Australia. (Oral communication)
11. Almeida, AM, Campos, A, Francisco, R, Van Harten, S, Cardoso, LA, Coelho, AV. 2009. Proteomic
profiling of the effects on weight loss on the rabbit’s gastrocnemius muscle - A study on two
different breeds. 3rd EuPA Congress, 14-17 June, Stockholm, Sweden.
12. Almeida, CMR, Dias, C, Mucha, AP, Bordalo, A, Vasconcelos, MTSD. 2009. Influence of surfactants
on the Cu phytoremediation potential of a salt marsh plant. BIOGEOMON 2009 - 6th
International Symposium on Ecosystem Behaviour, 29 June-3 July, Helsinki, Finland. (Oral
communication)
13. Almeida, J, Gravato, C, Guilhermino, L. 2009. Is cholinesterase activity inhibition linked to fish
swimming velocity decrease? Acute effects of fenitrothion on sea bass. SETAC North America
30th Annual Meeting, Human-Environment Interactions: Understanding Change in Dynamic
Systems, 19-23 November, New Orleans, USA.
14. Almeida, J, Gravato, C, Guilhermino, L. 2009. Which biomarkers responses are associated with
swimming velocity inhibition in the sea bass? A study with fenitrothion and benzo[a]pyrene.
SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the Challenge of a
Globally Changing Environment, 31 May-4 June, Gothenburg, Sweden.
15. Álvaro, NV, Neto, AI, Alves, MF, 2009. Human pressure on a protected area - a monitoring
program for the Islet of Vila Franca do Campo. ICS2009 - 10th International Coastal Symposium,
13-18 April, Lisbon, Portugal.
16. Álvaro, NV, Neto, AI, Alves, MF. 2009. Human pressure on a protected area - a monitoring
program for the Islet of Vila Franca do Campo. 10th International Coastal Symposium (ICS2009),
13-18 April, Lisbon, Portugal.
17. Alves Martins, D, Estévez, A, Stickland, NC, Simbi, BH, Yúfera, M. 2009. Effects of dietary marine
lecithin replacement on gilthead seabream (Sparus aurata) larvae. GIA2009 - Genomics in
Aquaculture 2009 Symposium, 5-7 July, Bodø, Norway.
18. Alves, RN, Richard, N, Cordeiro, O, Silva, TS, Marino, G, di Marco, P, Rodrigues, PM, Conceição,
LEC. 2009. Stressful rearing conditions and liver proteome expression in gilthead seabream
(Sparus aurata): identification of potential welfare indicators. GIA2009 - Genomics in
Aquaculture 2009 Symposium, 5-7 July, Bodø, Norway.
19. Amorim, A, Moita, MT, Brotas, V, Frazão, B, Palma, S, Rodrigues, SM, Silva, A, Veloso, V. 2009.
HABCOL - Colecção de culturas de microalgas marinhas nocivas-um recurso vital em estudos de
fitoplâncton. X Reunião Ibérica Fitoplâncton Tóxico e Biotoxinas, 12-15 May, Lisbon, Portugal.
20. Amorim, A, Moniz, M, Rodrigues, SM, Veloso, V, Frazão, B, Alves de Matos, AP, Ehrman, J,
Kaczmarzca, I. 2009. Espécies de Pseudo-nitzschia da Baía de Lisboa. X Reunião Ibérica
Fitoplâncton Tóxico e Biotoxinas, 12-15 May, Lisbon, Portugal.
21. Anjos, L, Redruello, B, Gomes, A, Melo, E, Canario, AVM, Power, DM. 2009. Acidic and secreted
protein in pituitary (ASPIP) - Biochemical and biophysical charactrerization of a novel pituitary
protein. IUPS - XXXVIth International Union of Physiological Sciences, 27 July-1 August, Kyoto,
Japan. The Journal of Physiological Sciences 59 (Supp 1).
22. Anjos, L, Reinhart, R, Gomes, A, Canario, AVM, Power, DM. 2009. Characterization of sea bream
vertebra proteome and effects of PTHrP. 7th AIEC Congress – Iberian Association of Comparative
Endocrinology, 6-9 September, Porto, Portugal. (Oral communication)
23. Antunes, A. 2009. Evolutionary genomic characterization of genes involved in development and
adaptation in vertebrates. EMBO Workshop - EVO-DEVO Meets Marine Ecology: New Frontiers in
Ocean Science through Integrative Biology, 9-11 October, Sant’Angelo d’Ischia, Naples, Italy.
24. Antunes, A. 2009. The role of genetics in the conservation of endangered mammalian species.
SEAZA - 17th Annual South East Asia Zoo Association Conference, 5-9 August, Seoul, South Korea.
(Oral communication)
25. Antunes, A. 2009.The evolutionary dynamics of the lion panthera leo revealed by host and viral
FIV population genomics. ISCB Africa and ASBCB Joint Conference on Bioinformatics of Infectious
Diseases, 30 November-3 December, Bamako, Mali. (Oral communication)
26. Aragão, C, Conceição, LEC, Pinto, W, Costas, B, Ribeiro, L, Dinis, MT. 2009. Amino acids in fish
nutrition: much more than the building blocks for growth. IMMR’09 - International Meeting on
Marine Resources, 16-18 November, Peniche, Portugal. (Oral communication)
27. Aragão, C, Weber, RA, Costas, B, Dinis, MT, Conceição, LEC. 2009. Effects of ammonia exposure in
Senegalese sole (Solea senegalensis) larvae. Proceedings of the Larvi’09 - Fish & Shellfish
Larviculture Symposium, 7-10 September, Ghent, Belgium. European Aquaculture Society,
Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P (Eds), Special Publication nº38: 14-17.
28. Araújo, R, Violante, J, Pereira, R, Abreu, H, Sousa-Pinto, I. 2009. Colonization of disturbed patches
in native communities by the introduced species Grateloupia turuturu. BIOLIEF - World
Conference on Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal.
29. Arenas, F, Fernández, C. 2009. Susceptibility to and impact of invasions on marine algal
assemblages: Experimental studies on invasion-stability relationships. BIOLIEF - World
Conference on Biological Invasion and Ecosystem Functioning, 27-30 October, Porto, Portugal.
30. Arnoso, J, Benavent, M, Bos, MS, Montesinos, FG. 2009. New insights into ocean tide loading
corrections on tidal gravity data in Canary Islands. EGU 2009, 19-24 April, Vienna, Austria. (Oral
Communication).
31. Assis, J, Tavares, D, Tavares, JT, Cunha, AH, Serrão, E. 2009. Findkelp, a GIS-based community
participation project to assess Portuguese kelp conservation status. International Coastal
Symposium, 14-17 April, Lisbon, Portugal.
32. Azevedo, C. 2009. A importância da protoparasitologia na fauna aquática. XV Jornada TécnicoCientíficas. INIPescas, 12-13 November, Luanda, Angola. (Oral communication)
33. Azevedo, C. 2009. Introdução: Estado da Arte. Mesa Redonda: Parasitologia e Patologia da Fauna
Aquática. XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal. (Oral
communication)
34. Azevedo, C. 2009. Protoparasitas: Morfologia e ultrastrutura do ciclo de vida. XV Jornada
Técnico-Científicas. INIPescas, 12-13 November, Luanda, Angola.
35. Azevedo, C, Casal, G, Matos, P, Matos, E. 2009. Estrutura fina de Triangulamyxa sp. (Myxozoa,
Myxosporea), parasita da bexiga urinária de Colomesus psittacus (Teleostei) do rio Amazonas. XI
Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal.
36. Azevedo, C, Casal, G, Mendonça, I, Carvalho, E, Matos, P, Matos, E. 2009. Dados ultrastruturais
do esporo de Myxobolus sp. (Phylum Myxozoa), parasita da guelra do peixe brasileiro Pimelodus
argenteus (Teleostei). XI Iberian Congress of Parasitology, 15-18 September, Lisboa, Portugal.
37. Azevedo, C, Marques, DKS, Amaral, CMC, Silva, EV, Casal, G, Matos, P, Matos, E. 2009.
Ultrastrutura do esporo de Henneguya sp. (Myxozoa) encontrado em Piaractus mesopotamicus
(Teleósteo) do Pantanal, MS, Brasil. XI Iberian Congress of Parasitology, 15-18 September, Lisbon,
Portugal.
38. Baptista, MS, Cyanca, RC, Almeida, CMR, Vasconcelos, VM. 2009. Determinação por
electroforese capilar do aminoácido não proteico ß-N-metilamino-L-alanina em meio de cultura
de cianobactérias. 1º Congresso Ibérico de Cianotoxinas, 6-7 July, Porto, Portugal.
39. Baptista, MS, Stoichev, T, Vasconcelos, VM, Vasconcelos, MTSD. 2009. Effects of the antibiotic
minocycline and the endocrine disrupting xenobiotic octylphenol in the cyanobacterium M.
aeruginosa. Microbial Stress: from Molecules to Systems, 7-10 May, Semmering, Austria.
40. Barrote, I, Albano, S, Santos, R, Silva, J. 2009. Oxidative stress in the intertidal seagrass Zostera
noltii. Mediterranean Seagrass Workshop, 6-10 September, Hvar, Croatia.
41. Bastos, L, Bio, A, Silva, AJ. 2009. Evolução da restinga do Douro face às condições meteorológicas,
hidrométricas e de agitação marítima, 2002-2008. Workshop: Douro and Minho – Estuaries and
Coastal Zone, 16-17 Abril, APDL, Matosinhos, Portugal. (Oral communication)
42. Bastos, L, Madeira, S, Gonçalves, J. 2009. A land based MMS for coastal monitoring. IAG2009
Assembly - Geodesy for Planet Earth, 31 August-4 September 2009, Buenos Aires, Argentina.
43. Bayona, J, Readman, J, Thomas, K, Beiras, R, Guilhermino, L. 2009. Implementation of risk
assessment methodologies for oil and chemical spills in the European marine environment
(RAMOCS). SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
Challenge of a Globally Changing Environment, 31 May-4 June, Gothenburg, Sweden.
44. Beirão, J, Pérez-Cerezales, S, Martínez-Páramo, S, Herráez, MP. 2009. Detection of early damages
in fish sperm cell membrane with YO-PRO 1. Second International Workshop on Biology of Fish
Gametes, 9-11 September, Valencia, Spain.
45. Bensimon-Brito, A, Cancela, ML, Huysseune, A, Witten, PE. 2009. Ontogeny of zebrafish (Danio
rerio) vertebral column and caudal skeleton - a model for vertebral fusion. 16th BENELUX
Congress of Zoology, 28-30 October, Wageningen, The Netherlands. (Oral communication)
46. Bensimon-Brito, A, Witten, PE, Cancela, ML, Huysseune, A. 2009. Ontogeny of zebrafish (Danio
rerio) caudal complex - a model for vertebral fusion. Interdisciplinary Approaches in Fish Skeletal
Biology, 27-29 April, Tavira, Portugal.
47. Bentes, L, Lino, PG, Abecasis, D, Santos, MN, Erzini, K. 2009. Using telemetry data to determine
factors influencing the choice of habitat. 8th Conference on Fish Telemetry, 14-18 September,
Umea, Sweden.
48. Bernabeu, AM, Almeida, CMR, Bouchette, Mucha, AP, Bordalo, AA and OILDEBEACH Team
(Álvarez, P, Azerad, P, Basto, MC, Bouharguane, A, Couto, N, Lago, A, Magalhães, C, Marche, F,
Mohamed, K, Mohammadi, B, Rey, D, Rubio, B, Vasconcelos, MTSD, Vilas, F). 2009. Development
of an assessment protocol to oiled sandy beaches: OILDEBEACH Project. ISMS 2009 - II
International Symposium of Marine Science, 27-30 April, Vigo, Spain. (Oral communication)
49. Bessa, J, Kijjoa, A, Wattanadilok, R, Puchakarn, S, Sonchaeng, P. 2009. Constituents of Petrosia
(Petrosia) hoeksema, collected from the Gulf of Thailand. 6th European Conference on Marine
Natural Products, 19-23 July, Porto, Portugal.
50. Bio, A, Couto, A, Costa, R, Prestes, A, Vieira, N, Valente, A, Azevedo, J. 2009. Biomanipulation of
Furnas Lake, Azores: effects of repeated fish removal. Proceedings of the International
Association of Theoretical and Applied Limnology - Verhandlungen Internationale Vereinigung für
Theoretische und Angewandte Limnologie 30(8): 1207-1209. (Oral communication).
51. Borges, M-T, Matos, A, Henriques, I, Ribeiro, H, Peixe, C, Pereira, CM, Castro, PML. 2009.
Variations of total bacteria and ammonia oxidizing bacteria community composition in a moving
bed biofilter of a shallow raceway fish farm. FEMS 2009 - 3rd Congress of European
Microbiologists, Microbes and Man - Interdependence and Future Challenges, 28 June-2 July,
Gothenburg, Sweden.
52. Borges, M-T, Mendes, JG, Restivo, MT, Pereira, CM. 2009. On-line water quality monitoring in
aquaculture: a remote laboratory proxy for environmental studies guidelines. Proceedings VU’09
- 10th International Conference Virtual University, 10-11 December, Bratislava, Slovakia, 6 pp.
53. Borges, R, Folhas, H, Franco, G, Gonçalves, EJ. 2009. Linking (or not) larval occurrence nearshore
with early life traits of a temperate cryptobenthic reef fish species from otolith analysis. 4th
International Otolith Symposium, 23-28 August, Monterey, California, USA. (Oral communication)
54. Borges, R, Serrão, E, Gonçalves, EJ. 2009. Different mechanisms for different species – small scale
differences in the occurrence and distribution of temperate reef fish larvae. 90th Annual Meeting
of the Western Society of Naturalists, 12-15 November, Seaside, Monterey, California, USA. (Oral
communication)
55. Borges, R, Vaz, J, Serrão, EA, Gonçalves, EJ. 2009 Short-term temporal fluctuation of verynearshore larval fish assemblages at the Arrábida Marine Park. 10th International Coastal
Symposium, 13-18 April 2009, Lisbon, Portugal. (Oral communication)
56. Borges, R, Vaz, J, Serrão, EA, Gonçalves, EJ. 2009. Tidal and vertical distribution of nearshore fish
larval assemblages at a temperate rocky shore. 33th Larval Fish Conference, 22-27 July, Portland,
USA. (Oral communication)
57. Bos, MS, Fernandes, RMS, Williams, SDP, Bastos, L. 2009. Fast analysis of GPS time-series with
gaps. American Geophysical Union - AGU Fall Meeting 2009, 14-18 December, San Francisco,
United States. Abstract G22A-03. (Oral communication)
58. Branco, M, Frazão, B, Moita, T. 2009. Estudo da toxicidade de Heterosigma akashiwo em Artémia
sp. X Reunião Ibérica Fitoplâncton Tóxico e Biotoxinas, 12-15 May, Lisbon, Portugal.
59. Cabral, JP. 2009. Darwin’s botanical work: unbound curiosity, robust methods and independent
thinking. Colóquio (Dis)entangling Darwin: Cross-Disciplinary Reflections on the Man and his
Legacy, 4-5 December, Porto, Portugal.
60. Cabrita, E, Sarasquete, C, Martinez-Paramo, S, Robles, V, Beirão, J, Cerezalez, C, Herráez, MP.
2009. Perspectives of cryopreservation of gametes and germinal cells. Workshop of
cryopreservation of marine organisms, 13-17 June, Coquimbo, Chile. (Invited Oral
communication)
61. Calado, C, Gonçalves, JMS, Santos, A, Queiroga, H. 2009. Potential use of decapod crustaceans to
detect unsual dynamics in coastal communities induced by climate changes. 3rd Estuarine &
Coastal Sciences Association International Symposium, 7-9 February, Lisbon, Portugal.
62. Caldeira, R, Chelius, A, Luis, E, Boutov, D, Sangra, P, Stegner, A. 2009. Archipelago Wake: Madeira
case study. Taller y Tertulia en Oceanogra a Física, 9-11 December, Universidad Las Palmas, Gran
Canarias, Spain. (Oral Communication)
63. Campos, A, Vasconcelos, V. 2009.Proteomics in the characterization of the toxicity mechanisms
of cyanotoxins in bivalves. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
64. Campos, C, Valente, LMP, Borges, P, Bizuayehu, T, Fernandes, JMO. 2009. Influence of dietary
lipids on growth and expression of key myogenic genes in Senegalese sole, Solea senegalensis
(Kaup). TAFT 2009 - 3rd Joint Trans-Atlantic Fisheries Technology Conference, 15-18 September,
Copenhagen, Denmark. (Oral presentation)
different conditions of water calcium availability in Tetraodon nigroviridis. IUPS - XXXVIth
International Union of Physiological Sciences, 27 July-1 August, Kyoto, Japan. The Journal of
Physiological Sciences 59 (Supp 1): 536.
66. Cancela, ML, Viegas, CSB, Laizé, V, Simes, DC. 2009. Fish as model organisms to uncover new Gla
proteins of interest for human biology. Interdisciplinary Approaches in Fish Skeletal Biology, 2729 April, Tavira, Portugal. (Oral communication)
67. Capela, RC, Hernadez, CS, Nunes, B, Correia, AT. 2009. The otoliths as potential chronological
markers of aquatic heavy metal contamination. 44th European Marine Biology Symposium, 7-11
September, Liverpool, England.
68. Capela, RC, Hernandez, CS, Nunes, B, Correia, AT. 2009. Trace metal incorporation in fish otoliths:
a chronological indicator of exposure to metal contamination. XII European Congress of
Icthyology, 6-12 September, Klaipeda, Lithuania.
69. Capela, T, Moreira, C, Vasconcelos, V. 2009. Toxicological evaluation by molecular analyses of
Cyanobacteria from Vez River, North of Portugal. 1st Iberian Congress in Cyanotoxins, 6-7 July,
Porto, Portugal.
70. Cardeira da Silva, J, Dionísio, G, Bensimon-Brito, A, Cancela, ML, Gavaia, PJ. 2009. Skeletal
development and performance of zebrafish Danio rerio (Hamilton, 1822) larvae and juvenile fish
fed with different diets. Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira,
Portugal.
71. Cardoso, C, Mendes, R, Salvador, A, Saraiva, J, Vaz-Pires, P, Nunes, ML. 2009. Influence of high
hydrostatic pressure on the quality parameters of hake (Merluccius capensis) gels. International
Meeting on Marine Resources, 16-18 November, Peniche, Portugal.
system. IUPS2009 - XXXVI International Congress of Physiological Sciences - Function of Life:
Elements and Integration, 27 July-1 August, Kyoto, Japan. The Journal of Physiological Sciences
59 (Supp 1): 98. (Oral communication)
73. Cardoso, JCR, Power, DM. 2009. Comparative evolution of the neuropeptide Y (NPY) and
PACAP/VIP family members. 7th AIEC Congress – Iberian Association of Comparative
74. Carrola, J, Fontaínhas-Fernandes, A, Matos, P, Carvalho, D, Parra, S, Rocha, E. 2009. Frequency of
hepatocellular fibrillar inclusions in the European flounder from the Douro river estuary,
Portugal. PRIMO 15 - 15th International Symposium on Pollutant Responses in Marine
Organisms, 17-20 May, Bordeaux, France.
75. Carrola, J, Fontaínhas-Fernandes, A, Pardal, M, Castro, B, Rocha, E. 2009. Intersex ratio in grey
mullets from the Mondego, Douro and Ave estuaries, Portugal - Preliminary data. PRIMO 15 15th International Symposium on Pollutant Responses in Marine Organisms, 17-20 May,
Bordeaux, France.
76. Carvalho, C, Martins, A, Vasconcelos, VM. 2009. Avaliação da toxicidade de cianobactérias em
simbiose com esponjas marinhas recorrendo à técnica de PCR. 1st Iberian Congress in
Cyanotoxins, 6-7 July, Porto, Portugal.
77. Carvalho, PN, Silva, MFGM, Basto, MCP, Machado, A, Bordalo, AA, Vasconcelos, MTSD. 2009.
Role of salt marsh plants on TBT biological remediation in sediments. 12th EuCheMs
International Conference on Chemistry and the Environment, 14-17 June, Stockholm, Sweden.
Book of Abstracts, p. 342.
78. Carvalho, PN, Silva, MFGM, Basto, MCP, Machado, A, Bordalo, AA, Vasconcelos, MTSD. 2009.
Role of salt marsh plants on TBT biological remediation in sediments. 12th EuCheMS
International Conference on Chemistry and the Environment, 14-17 June, Stockholm, Sweden.
Abstract book, p. 342.
79. Casal, G. 2009. Morfologia e filogenia de microsporidioses da ictiofauna. Mesa Redonda:
Parasitologia e Patologia da Fauna Aquática. XI Iberian Congress of Parasitology, 15-18
September, Lisbon, Portugal. (Oral communication)
80. Casal, G, Clemente, SS, Knoff, M, Matos, P, Matos, E, Azevedo, C. 2009. Ultrastrutura e filogenia
de Spraguea sp. (Filo Microsporidia Balbiani, 1882) parasita de Lophius gastrophysus do litoral de
Cabo Frio / RJ (Brasil). XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal.
81. Casal, G, Garcia, P, Matos, E, Teles-Grilo, L, Azevedo, C. 2009. Estudo ultrastrutural e molecular
de uma microsporidiose hepática causada por Microgemma sp., parasita do peixe Trachinotus
coralinus (Carangidae) do Sudeste do Brasil. XI Iberian Congress of Parasitology, 15-18
September, Lisbon, Portugal.
82. Castanheira, MF, Engrola, S, Conceição, LEC. 2009. Metabolic basis of growth variation in juvenile
sole (Solea senegalensis, Kaup 1858). IMMR`09 - International Meeting on Marine Resources, 1618 November, Peniche, Portugal.
83. Castro, LFC. 2009. Nuclear receptor evolution and endocrine disruption. In: Avances en
Endocrinología Comparada. 7th AIEC Congress – Iberian Association of Comparative
84. Catita, E, Martins, F, Antunes, C. 2009. Metodologias de Recolha de Informação e Zonamento
para Área Protegida - Costa Norte de Portugal. V Congresso sobre Planeamento e Gestão das
Zonas Costeiras dos Países de Expressão Portuguesa, 2 October, Florianopolis, Brazil.
85. Catita, E, Martins, F, Antunes, C. 2009. Metodologias de recolha de informação e zonamento
para área protegida - Costa Norte de Portugal. V Congresso sobre Planeamento e Gestão das
Zonas Costeiras dos Países de Expressão Portuguesa, 30 September-2 October, Florianopolis,
Brazil.
86. Cavaleiro, F, Correia, AM, Santos, MJ. 2009. Seasonal occurrence of gastrointestinal helmithosis
in European flounder, Platichthys flesus (Linnaeus, 1758) (Teleostei: Pleuronectidae), of marine
environments of the northwest region of the Portuguese coast. XI Iberian Congress of
Parasitology, 158-18 September, Lisbon, Portugal. Acta Parasitológica Portuguesa 16(1/2): 380381.
87. Cavaleiro, F, Rangel, L, Santos, MJ. 2009. Copepoda ectoparasites of some commercially
important fish species of the Portuguese coast. XI Iberian Congress of Parasitology, 15-18
September, Lisbon, Portugal. Acta Parasitológica Portuguesa 16(1/2): 378-379.
88. Cavaleiro, F, Santos, MJ. 2009. Seasonal occurrence of metacercariae of the genus Diplostomum
von Nordmann, 1832 in the eyes of the European flounder, Platichthys flesus (Linnaeus, 1758)
(Teleostei: Pleuronectidae), caught of the northwest Portugal. XI Iberian Congress of
Parasitology, 15-18 September, Lisbon, Portugal.Acta Parasitológica Portuguesa 16(1/2): 382383.
Hydrozoa) and Palaemon macrodactylus (Crustacea, Decapoda): potential impacts and mitigation
measures. BIOLIEF - World Conference on Biological Invasions and Ecosystem Functioning, 27-30
October, Porto, Portugal.
90. Coelho, R, Hazin, FHV, Rego, M, Tambourgi, M, Oliveira, P, Travassos, P, Carvalho, F, Burgess, G.
2009. Abundance, distribution and reproductive biology of the oceanic whitetip shark caught by
the tuna pelagic longline fishery in the southwest Atlantic Ocean. 25th American Elasmobranch
Association Meeting, 22-27 July, Portland, Oregon, USA.
91. Coelho, R, Oliveira, P, Hazin, F, Carvalho, F, Rego, M, Piercy, A, Burgess, G. 2009. Abundance, atvessel fishing mortality, distribution and reproductive biology of the crocodile shark,
Pseudocarcharias kamoharai, in the south west Atlantic Ocean. 8th Indo Pacific Fish Conference
and 2009 ASFB Workshop & Conference, 31 May-5 June, Fremantle, WA, Australia.
92. Colen, R, Rodrigues, V, Araújo, L, Afonso, A, Dias, J. 2009. Effects of a dietary supplementation of
beta-glucans and algae extracts on the immune response and oxidative status of Senegalese sole
(Solea senegalensis) juveniles. IMMR`09 - International Meeting on Marine Resources, 16-18
November, Peniche, Portugal.
93. Conceição, LEC, Aragão, C, Costas, B, Terova, G, Dias, J, Martins, C, Tort, L. 2009. Dietary nitrogen
and fish welfare. COST Action 867 Meeting, 15-17 June, Isafjordur, Iceland. (Oral communication)
94. Conceição, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2009. Avanços
recentes em nutrição de larvas de peixes. 46th Anual meeting of the Brazilian Society of
Zootechnology, 14-17 July, Maringá, Paraná, Brazil. (Invited Oral communication).
95. Conceição, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2009. Avanços
recentes em nutrição de larvas de peixes. 46th Anual meeting of the Brazilian Society of
Zootechnology, 14-17 July, Maringá, Paraná, Brazil. (Invited Oral communication)
96. Conceição, LEC, Rønnestad, I. 2009. A dynamic model for dietary amino acids utilisation in fish
larvae. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium, 7-10 September,
Ghent, Belgium. European Aquaculture Society, Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos,
P (Eds), Special Publication nº38. (Oral communnication).
97. Conceição, N, Cox, C, Simões, B, Viegas, M, Cancela, ML. 2009. Comparative promoter analysis
and its application in analysis of cartilage-expressed genes regulation. Workshop Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
98. Correia, AT, Gomes, P, Gonçalves, JMS, Karim, E, Hamer, PA. 2009. Stock discrimination of black
seabream (Spondyliosoma cantharus) along the SW Portuguese coast through otolith elemental
fingerprinting. XII European Congress of Icthyology, 6-12 September, Klaipeda, Lithuania. (Oral
communication)
99. Costa, R, Keller, T, van Overbeek, L, van Elsas, JD. 2009. Bacterial diversity in the freshwater
sponge Ephydatia fluviatilis. 10th Symposium on Bacterial Genetics and Ecology, 15-19 June,
Uppsala, Sweden.
100. Costa, S, Martins, R, Ramos ,V, Vasconcelos, VM..2009. Ensaios ecotoxicológicos com estirpes de
cianobactérias marinhas do género Leptolyngbya. 1st Iberian Congress in Cyanotoxins, 6-7 July,
Porto, Portugal.
101. Costa-Dias, S, Dias, E, Lobón-Cerviá, J, Antunes, C, Coimbra, J. 2009. Infection by Anguillicola
crassus in a riverine stock of European eel Anguilla anguilla (L.). World Conference on Biological
Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal. Book of Abstracts, p. 98.
102. Costa-Dias, S, Dias, E, Lobón-Cerviá, J, Antunes, C, Coimbra, J. 2009. Infection by Anguillicola
crassus in a riverine stock of European eel Anguilla anguilla (L.). BIOLIEF - World Conference on
Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal. Book of
Abstracts, p. 98.
103. Costas, B, Conceição, L, Aragão, C, Martos, JA, Ruiz-Jarabo, I, Mancera, JM, Afonso, A. 2009. How
does an acute stress modulate the innate immune system of Senegalese sole (Solea senegalensis
Kaup 1858)?. 7th AIEC Congress – Iberian Association of Comparative Endocrinology, 6-9
September, Porto, Portugal.
104. Costas, B, Conceição, L, Aragão, C, Martos, JA, Ruíz-Jarabo, I, Mancera, JM, Afonso, A. 2009.
Innate immune cells of Senegalese sole (Solea senegalensis Kaup 1858): effects of handling stress
on respiratory burst activity. 11th International Congress of the ISDCI, 28 June-4 July, Prague,
Czech Republic.
105. Couto, MNF, Basto, MCP, Vasconcelos, MTSD. 2009. A study in soil of a refinery of the efficiency
of three different plant species for petroleum hydrocarbons remediation. 6th International
Phytotechnologies Conference 2009, 1-4 December, Missouri, USA.
106. Couto, MNF, Vasconcelos, MTSD. 2009. Phytoremediation of hydrocarbons from a refinery’s soil the role of rhizosphere. International Symposium on Contaminated Soils and Sediments 2009,
23-25 September, Ferrara, Italy.
107. Couto, RP, Neto, AI, Rodrigues, AS. 2009. Metals concentration and structural changes in
Corallina elongata (Corallinales, Rhodophyta) from hydrothermal vents. 7th Asia-Pacific
Conference on Algal Biotechnology, 1-4 December, Delhi, India.
108. Cox, CJ. 2009. Phylogenetic analyses of the chloroplast proteomes of charophytes and the major
land plant groups using methods to account for non-stationary composition heterogeneity.
International Association of Bryology, 16-21 August, Cape Town, South Africa. (Pleanary lecture)
109. Cristiano, MLS. 2009. New approaches to antimalarial drug design: plasmodium DNA-directed
endoperoxides. 2º Encontro da Plataforma Ibérica da Malaria, 17-18 December, GSK, Tres
Cantos, Madrid, Spain. (Oral communication)
110. Cruz, C, Saraiva, A, Santos, MJ, Eiras, JC, Ventura, C, Soares, JP, Hermida, M. 2009. Parasitose
provocada por larvas de Anisakis spp. (NEMATODA: ANISAKIDAE) no peixe de espada preto
Aphanopus carbo (OSTEICHTHYES: TRICHIURIDAE) em Portugal. XI Iberian Congress of
Parasitology, 15-18 September, Lisbon, Portugal. Acta Parasitológica Portuguesa 16(1/2): 172173.
111. Cruz, J, Chícharo, AM, Garrido, S, Ben-Hamadou, R, Chícharo, L, Ré, P, Santos, AMP. 2009.
Temporal and spatial variation of egg production, RNA:DNA ratio and fatty acids composition of
the calanoid copepod Acartia clausi in the Gulf of Cadiz. ICES Annual Science Conference - ASC,
21-25 September, Berlin, Germany.
112. Cruzeiro, C, Rema, P, Dias, J. 2009. O uso de anticorpos como aditivo na produção de truta arcoíris (Onchorhynchus Mykiss). XVIII Congress of Zootecnic and II Iberoamerican Congress of
Zootecnic, University of Trás-os-Montes and Alto Douro, 6-9 May, Vila Real, Portugal. Abstract
Book, pp. 555-558. ISBN 978-989-96219-1-6.
113. Cruzeiro, C, Rema, P, Peixoto, F, Pires, M, Afonso, A. 2009. The anti-phospholipase effects at
muscle lipid oxidation level; histological and immunological parameters when applied to rainbow
trout (Onchorhynchus mykiss) feed. XVIII Congress of Zootecnic and II Iberoamerican Congress of
Zootecnic, University of Trás-os-Montes and Alto Douro, 6-9 May, Vila Real, Portugal. Abstract
Book, pp. 564-568. ISBN 978-989-96219-1-6.
114. Cruzeiro, L. 2009. Proteins are quantum mechanical machines. QuEBS09 - Quantum Effects in
Biological Systems, 7-10 July, Lisbon, Portugal. (Oral communication)
115. Cruzeiro, L. 2009. The VES Hypothesis and Protein Folding and Misfolding. LENCOS - Localized
Excitations in Nonlinear Complex Systems, 14-18 July, Seville, Spain. (Oral communication)
116. Cunha, A, Assis, J, Serrao, E. 2009. Conservation status of Portuguese seagrases: are seagrass
habitats one of the most endangered marine habitats?. Mediterranean Seagrass Workshop, 6-10
September, Hvar, Croatia. (Oral communication)
117. Cunha, AH, Assis, J, Serrao, E. 2009. Estimation of available seagrass meadow area in Portugal for
transplanting purposes. International Coastal Symposium, 14-17 April, Lisbon, Portugal.
118. Cunha, AH, Erzini, K, Serrão, E, Gonçalves, E, Henriques, M, Henriques, V, Guerra, M, Duarte, C,
Marbá, N, Fonseca, M. 2009. Biomares: a LIFE project to restore and manage the biodiversity of
Luiz Saldanha Marine Park. International Meeting of Marine Resources, 16-18 November,
Peniche, Portugal. (Oral communication).
119. Cunha, AH, Paulo, D, Rodrigues, S, Boavida, J, Fonseca, M. 2009. Open ocean restoration.
Mediterranean Seagrass Biology Workshop, 1-5 September, Hvar, Croatia. (Oral communication)
120. Cunha, AH, Serrão, E, Assis, J. 2009. Status of Portuguese Seagrasses: three levels of proactive
management. 20th Biennial Conference of the Coastal and Estuarine Research Federation, 1-5
November, Portland, Oregon, USA, (Oral communication)
121. Cunha, ME, Quental Ferreira, H, Barradas, A, Pousão-Ferreira, P, Cabrita, E, Engrola, S. 2009.
Growth and developement od Dusky grouper Epinephelus marginatus larvae in mesocosm of
semi-intensive technology. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium,
7-10 September, Ghent, Belgium. European Aquaculture Society, Hendry, CI, Van Stappen, G,
Wille, M, Sorgeloos, P (Eds), Special Publication nº38: 52-55.
122. Deloffre, L, Lesage, J, Power, D, Canario, A. 2009. Characterisation of growth and differentiation
factor-9 in Oreochromis mossambicus ovary and gene expression during follicle growth. 7th AIEC
Congress – Iberian Association of Comparative Endocrinology, 6-9 September, Porto, Portugal.
123. Dethoup, T, Manoch, L, Buaruang, J, Piriyaprin, S, Kijjoa, A. 2009. The In Vitro antagonitic effect of
marine sponge-associated fungi against plant pathogenic fungi. 6th European Conference on
Marine Natural Products, 19-23 July, Porto, Portugal.
124. Deurloo, R, Bastos, L, Bos, MS. 2009. On the use of UAVs for strapdown airborne gravimetry.
IAG2009 Assembly - Geodesy for Planet Earth, 31 August-4 September, Buenos Aires, Argentina.
125. Dias, A, Costa-Dias, S, Antunes, C. 2009. Intermediary and paratenic hosts of the parasitic
nematode Anguillicola crassus. World Conference on Biological Invasions and Ecosystem
Functioning, 27-30 October, Porto, Portugal. Book of Abstracts, p. 26
126. Dias, J, Conceição, L, Ribeiro, AR, Matos, E, Gonçalves, A, Borges, P, Valente, L, Dinis, MT. 2009.
Development of sustainable feeds for gilthead seabream (Sparus aurata) during the grow-out
stage: growth performance, feed utilization, flesh quality and soluble wastes. IMMR’09 -
International Meeting on Marine Resources, 16-18 November, Peniche, Portugal. (Oral
communication)
127. Dionísio, G, Bensimon-Brito, A, Gavaia, PJ, Cancela, ML. 2009. Effect of warfarin in zebrafish
(Danio rerio) bone formation during caudal fin regeneration. Interdisciplinary Approaches in Fish
Skeletal Biology, 27-29 April, Tavira, Portugal.
128. Duarte, D, Francisco, C, Almeida, A, Castro, A, Santos, MJ. 2009. Genetic identification of
Didymobothrium rudolphii (Cestode: Spatheobothriidea) morphotypes “frontal” and “lateral” in
the Sand sole, Solea lascaris (Risso, 1810). XI Iberian Congress of Parasitology, 15-18 September,
Lisbon, Portugal. Acta Parasitológica Portuguesa 16(1/2): 388-389.
129. Duarte, D, Santos, MJ. 2009. Parasitism in Talorchestia brito Stebbing, 1891 of the Northern and
Central coast of Portugal. XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal.
Acta Parasitológica Portuguesa 16(1/2): 384-385.
130. Eiras, JC. 2009. Lesões em peixes provocadas por parasitas. XI Iberian Congress of Parasitology,
15-18 September, Lisbon, Portugal.
131. Eiras, JC. 2009. Parasitoses e qualidade do pescado. XII Foro dos Recursos Mariños e da
Acuicultura das Rías Galegas, 8-9 October, La Toja, Spain.
132. Engelen, A, Santos, R. 2009. Comparing invader associated fauna in the native and introduced
region. the case of the brown seaweed Sargassum muticum. BIOLIEF - World Conference on
Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal.
excellence. 9th International Phycological Congress, 2-8 August, Tokyo, Japan. Phycologia 48(4):
29.
134. Engelen, AH. 2009. Brown macroalgal invaders of the genus Sargassum: flexibility without
compromise? Invited seminar, University of Plymouth, Plymouth, England. (Invited lecture)
135. Engelen, AH. 2009. Demographic matrix modelling of marine populations and communities under
stress. Invited seminar, ZMT Bremen, Germany. (Invited lecture)
136. Engelen, AH. 2009. Invasion ecology of Sargassum muticum, important aspects of the invader par
excellence. Invited seminar, Institute of Marine Sciences School of Biological Sciences University
of Portsmouth, Porthsmouth, England. (Invited lecture)
137. Engrola, S, Dinis, MT, Conceição, LEC. 2009. Senegalese sole is able to adapt protein metabolism
when co-fed with Artemia replacement. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture
Symposium, 7-10 September, Ghent, Belgium. European Aquaculture Society, Hendry, CI, Van
Stappen, G, Wille, M, Sorgeloos, P (Eds), Special Publication nº38: 84-87.
138. Erzini, K, Coelho, R, Abecasis, R, Sousa, I, Gonçalves, JMS. 2009. Effects of protection from fishing
in the Luiz Saldanha Marine Park: differences in catch per unit effort, mean size, elasmobranch
catches and total value of trammel net catches between three protection levels. International
Meeting on Marine Resources, 16-18 November, Peniche, Portugal.
139. Esgaio, E, Cardoso, JCR, Power, DM. 2009. Thyroid hormone cell transporters: do they exist in
fish?. 7th AIEC Congress – Iberian Association of Comparative Endocrinology, 6-9 September,
Porto, Portugal.
140. Estrada-Allis, S, Machín, F, Santana, A, Sangrà, P, Couvelard, X, Caldeira, R. 2009. Far-field model
validation using ARGO data: toward the construction of a regional system. Taller y Tertulia en
Oceanogra a Física, 9-11 December, Universidad Las Palmas, Gran Canarias, Spain.
141. Fazenda, C, Viegas, CSB, Conceição, N, Simes, DC, Cancela, ML. 2009. Development of molecular
and cellular tools to study the function of skeletal Gla-containing proteins in sturgeon. Workshop
- Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
142. Fernandes, C, Fontaínhas-Fernandes, A, Coimbra, A, Saavedra, MJ, Salgado, MA. 2009.
Biochemical blood parameters: are they effective biomarkers of chronic toxicity?. Proceedings of
the SECOTOX Conference and CEMEPE - International Conference on Environmental
Management, Engineering, Planning and Economics, 21-26 June, Mykonos, Greece. Kungolos, A,
Aravossis, K, Karagiannidis, A, Samaras, P (Eds), Vol. I, pp. 191-196. ISBN 978-960-6865-09-1.
143. Fernandes, J, Lázaro, C, Bastos, L, Pires, N, Nunes, A, Mendes, V, Barbosa, S, Cipollini, P. 2009.
GNSS-derived path delay: a method to compute the wet tropospheric correction for coastal
altimetry. 3rd Coastal Altimetry Workshop, 17-18 September, Frascati, Italy. (Oral
communication)
144. Fernandes, JMO, Campos, C, Valente, LMP. 2009. Epigenetic regulation of growth by dietary
lipids in Senegalese sole (Solea Senegalensis). Asian Pacific Aquaculture 2009, 4-6 November,
Kuala Lumpur, Malaysia. (Oral communication)
145. Fernandes, M, Canário, A, Costa, MC. 2009. Isolation of high performance indigenous microbial
consortia from highly acidic acid mine drainage contaminated soils. Microbioworld 09, 2-4
December, Lisbon, Portugal.
146. Fernandes, RMS, Msemwa, J, Bos, M, and the KILI2008 Team. 2009. The KILI2008 project: Precise
measurement of the highest elevation of Africa: Mt. Kilimanjaro. European Geophysical Union EGU 2009, 19-24 April, Vienna, Austria.
147. Fernández, I, Tiago, DM, Laizé, V, Cancela, ML, Gisbert, E. 2009. Effect of retinoic acid on the
proliferation and in vitro mineralization of seabream bone-derived cell lines. Interdisciplinary
Approaches in Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
148. Ferraz de Arruda, L, Ozorio, ROA, Vaz-Pires, P, Ramos, B, Gonçalves, JF, Oetterer, M. 2009.
Resíduos do processamento de pescado na formulação de rações para aquicultura. 2º Simpósio
de Pós-Graduandos do CENA/USP - Centro de Energia Nuclear na Agricultura, 25 September,
University of São Paulo, Brazil. (Oral communication)
149. Ferraz de Arruda, L, Vaz-Pires, P, Ozorio, R, Julião, L, Savay da Silva, L, Angelini, MF, Galvão, JÁ,
Oetterer, M. 2009. Utilização de resíduos da indústria de conservas de Porto - Portugal para
produção de silagem ácida de pescado. 2º Simpósio de Pós-Graduandos do CENA/USP - Centro
de Energia Nuclear na Agricultura, 25 September, University of São Paulo, Brazil. (Oral
communication)
150. Ferreira, C, Brandão, R, Pinto, L, Radhouani, H, Igrejas, G, Poeta, P, Martins da Costa, P. 2009.
Emergência de estirpes de Escherichia coli multi-resistentes aos antimicrobianos em aves
selvagens. VI SPEA Congress of Ornithology and IV Iberian Congress of Ornithology, 5-8
December, Elvas, Portugal.
151. Figueiredo, D, Coelho, C, Heitor, A, Osswald, J, Vasconcelos, V, Ramos, I, Pereira, M, Correia, A.
2009. Multilevel characterization of cyanobacterial summer blooms in three shallow lakes proposal for a monitoring strategy. Bageco 2009 - 10th International Symposium on Bacterial
Genetics and Ecology, 23-27 June, Uppsala, Sweden.
152. Filho, TUB, Loureiro, S, Gravato, C, Guilhermino, L, Sousa, E, Gasparro, M, Semmler, M. 2009. The
use of biomarkers in fish as indicators of contamination in estuarine environments: the case
study of Santos and São Vicente estuarine systems, SP, Brazil. SETAC Europe 19th Annual
Meeting - Protecting Ecosystem Health: Facing the Challenge of a Globally Changing
Environment, 31 May-4 June, Gothenburg, Sweden.
153. Filipe, AIR, Miguel, MG, Cardoso, JCR, Power, DM, Marques, NT. 2009. Isolation of a member of
the terpene sythase enzyme family from Thymus mastichina and Thymus albicans. 8th Plant
Genomics European Meeting, 7-10 October, Lisbon, Portugal.
154. Francisco, CJ, Almeida, A, Castro, AM, Santos, MJ. 2009. Molecular and morphological data of the
Diphtherostomum brusinae (Trematoda: Zoogonidae) infecting Mytilus galloprovincialis from
Aveiro Estuary, Portugal. XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal.
Acta Parasitológica Portuguesa 16 (1/2): 104-105.
155. Francisco, CJ, Almeida, A, Castro, AM, Santos, MJ. 2009. Molecular data of adult worms of
Prosorhynchus crucibulum and Prosorhynchus aculeatus: sensitivity of a PCR method. XI Iberian
Congress of Parasitology, 15-18 September, Lisbon, Portugal. Acta Parasitológica Portuguesa
16(1/2): 102-103.
156. Francisco, CJ, Hermida, MA, Santos, MJ. 2009. It is the miracidium of Prosorhynchus crucibulum a
passively infecting larva? XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal.
Acta Parasitológica Portuguesa 16 (1/2): 100-101.
157. Francisco, CJ, Hermida, MA, Santos, MJ. 2009. Survey of parasites from Mytilus galloprovincialis
of the Aveiro Estuary, Portugal. XI Iberian Congress of Parasitology, 15-18 September, Lisbon,
Portugal. Acta Parasitológica Portuguesa 16 (1/2): 98-99.
158. Freitas, MA, Regueiras, A, Martins, AJ, Moreira, CI, Antunes, A, Vasconcelos, VM. 2009.
Comparação de diferentes métodos de detecção e quantificação de cianobactérias e suas
toxinas. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
159. Fuentes, J, Brinca, L, Guerreiro, PM, Power, DM. 2009. PRL and GH turnover in the pituitary gland
of the sea bream (Sparus auratus L.) in response to freshwater challenge. 7th AIEC Congress –
Iberian Association of Comparative Endocrinology, 6-9 September, Porto, Portugal. (Oral
communication)
160. Gamez-Zavaglia, IA, Cabral, L-I-L, Cristiano, MLS. 2009. Molecular structure and reactivity of novel
saccharinate-tetrazoles used as multidentate ligands. XIIth European Symposium on Organic
Reactivity, 6-12 September, Haifa, Israel. (Oral communication)
161. Gaspar, MB, Rufino, MM, Vasconcelos, P. 2009. Bivalves and sediment type: distribution along
the south-eastern coast of Algarve. 5th International Symposium on Sandy Beaches, 19-23
October, Rabat, Marocco.
162. Gavaia, PJ, Richard, N, Dâmaso, L, Dinis, MT, Pousão-Fereira, P, Engrola, S, Conceição, L, Cancela,
ML. 2009. Skeletal evaluation and technical improvements to decrease the incidence of skeletal
deformities in Solea senegalensis, Kaup. Interdisciplinary Approaches in Fish Skeletal Biology, 2729 April, Tavira, Portugal. (Oral communication)
163. Gavaia, PJ, Richard, N, Dâmaso, L, Dinis, MT, Pousão-Fereira, P, Engrola, S, Conceição, L, Cancela,
ML. 2009. Skeletal evaluation and technical improvements to decrease the incidence of skeletal
deformities in Solea senegalensis, Kaup. Interdisciplinary Approaches in Fish Skeletal Biology, 2729 April, Tavira, Portugal. (Oral communication)
164. Gil, I, Keigwin, L, Abrantes, F. 2009. Comparison of diatom records of the Heinrich Event 1 in the
Western North Atlantic. PAGES 1st Young Scientific Meeting, 6-7 July, Corvallis, USA.
165. Gomes, AS, Fuentes, J, Cardoso, J, Power, DM, Canário, AVM. 2009. Calciotropic effect of the PTH
family of peptides in the clawed frog. 7th AIEC Congress – Iberian Association of Comparative
Endocrinology, 6-9 September, Porto, Portugal.
166. Gomes, S, Amorim, MJB, Novais, S, Gravato, C, Guilhermino, L, Soares, AMVM, Scott-Fordsmand,
J. 2009. Effects of Cu-nanoparticles versus Cu 2+ in Enchytraeus albidus (Oligochaeta):
differencitial gene expression and stress biomarker responses. SETAC Europe 19th Annual
Environment, 31 May-4 June, Gothenburg, Sweden. (Oral communication)
167. Gonçalves, EJ, Borges, R, Folhas, H, Franco, G. 2009. Planktonic larval duration of eleven species
of temperate cryptobenthic reef fishes: relation with possible dispersive and retention patterns
at the Arrábida Marine Park. 4th International Otolith Symposium, 23-28 August, Monterey,
California, USA. (Oral communication)
168. Gonçalves, EJ, Borges, R. 2009. Interannual fluctuations in the structure of temperate reef-fish
larval assemblages at the Arrábida Marine Park. 33rd Annual Larval Fish Conference, 23-27 July
2009, Portland, Oregon, USA. (Oral communication)
169. Gonçalves, JA, Madeira, S, Bastos, L. 2009. Application of a low cost mobile mapping system to
coastal monitoring. MMT09 - 6th International Symposium on Mobile Mapping Technology, 2124 July, São Paulo, Brazil. (Oral communication)
170. Gonçalves, JMS, Erzini, K, Monteiro, P, Rangel, M, Veiga, P, Afonso, C, Oliveira, F, Bentes, L. 2009.
Mapping of marine subtidal communities as a tool for a conservation and sustainable use of
coastal biodiversity. International Meeting on Marine Resources, 16-18 November, Peniche,
Portugal.
171. Gouveia, K, Vaz-Pires, P, Martins da Costa, P. 2009. Avaliação do bem-estar de frangos através do
exame post mortem. XVIII Congress of Zootecnic and II Iberoamerican Congresso f Zootecnic,
University of Trás-os-Montes and Alto Douro, 6-9 May, Vila Real, Portugal.
172. Gravato, C, Almeida, J, Oliveira, C, Oliveira, P, Luis, L, Guilhermino, L. 2009. Linking biochemical
tools with new and simple behaviour tests: results obtained with marine organisms exposed to
PAHs, pesticides and pharmaceuticals. SETAC North America 30th Annual Meeting, 19-23
November, New Orleans, USA. (Oral communication)
173. Gravato, C, Almeida, JR, Vandenbrouck, T, Van der Ven, K, De Coen, W, Guilhermino, L. 2009.
Linking gene expression, biomarkers and behaviour alterations induced by benzo(a)pyrene on
sea bass. ECNIS - Environmental Cancer Risk, Nutrition and Individual Susceptibility, International
Workshop on Biomarkers and Cancer, 21-23 September, Porto, Portugal. (Oral communication)
174. Gravato, C, Freylan, M, Almeida, J, Oliveira, P, Luís, L, Oliveira, C, Castillo, L, Guilhermino, L. 2009.
Effects of mancozeb on key enzymes of the common goby (Pomatoschistus microps): what can
we expect for wild tropical gobies? SETAC North America 30th Annual Meeting, HumanEnvironment Interactions: Understanding Change in Dynamic Systems, 19-23 November, New
Orleans, USA.
175. Gravato, C, Mena, F, Almeida, J, Oliveira, P, Oliveira, C, Luís, L, Castillo, M, Guilhermino, L. 2009.
Acute effects of the pesticide mancozeb on the estuarine fish Pomatoschistus microps. SETAC
Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the Challenge of a Globally
Changing Environment, 31 May-4 June, Gothenburg, Sweden.
176. Guilhermino, L. 2009. Environmental biomarkers and ecological relevance: is this important for
the marine environment?. E3 - 2nd Expert Examination and Evaluation Workshop: Biomarkers which roles can they play in environmental management related to oil and gas activities offshore? 26-27 November, Paris, France. (Invited Oral communication)
177. Guimarães, L, Afonso, MJ, Chaminé, HI, Marques, JM, Guilhermino, L. 2009. Assessing urban
groundwater from spring catchworks of Porto city (NW Portugal) using acetylcholinesterase
inhibition tests. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
178. Guimarães, L, Gravato, C, Santos, J, Guilhermino, L. 2009. Is pollution affecting the health status
of wild Anguilla anguilla populations? A case study in NW Portuguese estuaries. SETAC North
America 30th Annual Meeting, Human-Environment Interactions: Understanding Change in
Dynamic Systems, 19-23 November, New Orleans, USA.
179. Guimarães, L, Gravato, C, Sousa, A, Guilhermino, L. 2009. Monitoring the effects of estuarine oilderived pollution on wild populations of common goby Pomatoschistus microps. 15th
International Symposium on Pollutant Responses in Marine Organisms, 17-20 May, Bordeaux,
France.
180. Guimarães, L, Guilhermino, L. 2009. Acute toxicity test with Daphnia magna: an alternative to
mammals in the prescreening of chemical toxicity?. Workshop on Alternative methods accessing
non-target infectivity and toxicity of microbial plant protection products and biocides. Cost
Action 862 - Bacterial Toxins for Insect Control, WG5 Workshop Salzau Castle, 26 March,
Germany. (Invited Oral communication)
181. Guimarães, L, Neuparth, T, Gravato, C, Guilhermino, L. 2009. Lipid peroxidation and oxidative
stress biomarkers in natural populations of Carcinus maenas (NW portugal) with different
contamination histories. 15th International Symposium on Pollutant Responses in Marine
Organisms, 17-20 May, Bordeaux, France.
182. Guimarães, L, Neuparth, T, Gravato, C, Guilhermino, L. 2009. Morphometric and biochemical
variability in male populations of Carcinus maenas from two NW Portuguese estuaries with
different pollution levels. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health:
Facing the Challenge of a Globally Changing Environment, 31 May-4 June, Gothenburg, Sweden.
183. Hardoim, CCP, Costa, R, Araújo, FV, Hajdu, E, Peixoto, R, Lins, U, Rosado, AS, van Elsas, JD. 2009.
Bacterial diversity in the marine sponge Aplysina fulva in brazilian coastal waters. 10th
Symposium on Bacterial Genetics and Ecology, 15-19 June, Uppsala, Sweden.
184. Hardoim, CCP, Costa, R, Lins, U, Peixoto, R, Rosado, AS, van Elsas, JD. 2009. Diversity of the
candidate phylum Poribacteria associated with the marine sponge Aplysina fulva in southeast
Brazil. 10th Symposium on Bacterial Genetics and Ecology, 15-19 June, Uppsala, Sweden.
185. Hernández-Garcia, A, García-Fernández, AJ, Garcia, DR, Gravato, C, Guilhermino, L. 2009.
Eritrocitos de dos especies de aves rapaces para la evaluación de esters oxidativo inducido por la
exposición única y conjunta a cadmio y plomo in vitro. XVIII Congreso Español de Toxicologia, 911 September, Palma Mallorca, Spain. (Oral communication)
186. Hernández-Garcia, A, Garcia, DR, Gravato, C, Guilhermino, L, García-Fernández, AJ. 2009.
Caracterización de parámetros bioquímicos asociados a los mecanismos antioxidantes celulares
en eritrocitos de tres especies de aves silvestres. XVIII Congreso Español de Toxicologia, 9-11
September, Palma Mallorca, Spain.
187. Herrera, M, Aragão, C, Hachero, I, Ruíz-Jarabo, I, Vargas-Chacoff, L, Mancera, JM, Conceição, L.
2009. Effects of sudden salinity changes on the oxygen consumption and osmoregulatory
parameters in the Senegalese sole (Solea senegalensis Kaup, 1858). Scottish Aquaculture: a
Sustainable Future, 21-22 April, Edinburgh, UK.
188. Howcroft-Ferreira, C, Amorim, MJB, Gravato, C, Guilhermino, L, Soares, AMVM. 2009. Neuromuscular and oxidative stress biomarkers in Enchytraeus albidus and linkage to population and
behaviour level effects. Young Environmental Scientists Meeting, 16-18 March, Landau,
Germany. (Oral communication)
189. Huertas, M, Canario, AV, Hubbard, PC. 2009. Follow your nose: Chemical communication
throughout the European eel (Anguilla anguilla, L.) life-cycle. Annual Meeting of the Society for
Experimental-Biology, 28 June-1 July 2009, Glasgow, Scotland. Comparative Biochemistry and
Physiology A - Molecular & Integrative Physiology 153A(2): S90-S90. (Oral communication).
190. Huertas, M, Hubbard, PC, Canário, AVM. 2009. Novel sex steroid production in testicular tissue of
the european eel (Anguilla anguilla, L.). 16th International Congress of Comparative
Endocrinology 2009, 22-26 June, Hong Kong, China.
191. Huertas, M, Li, W. 2009. Is there a steroid binding protein system in the sea lamprey?. 16th
International Congress of Comparative Endocrinology 2009, 22-26 June, Hong Kong, China.
192. Imhoff, JL, Burgess, GH, Fernandez-Carvalho, J, Romine, J, Bester, C, Coelho, R. 2009. Endangered
U.S. sawfishes (Pristis spp.): conservation, research and education. 29th Annual Meeting of the
Florida Chapter of the American Fisheries Society, 17-19 February, Ocala, Florida, USA.
193. Jiménez, ALO, Soler, F, Pérez-López, M, Zalba, J, Gravato, C, Guilhermino, L. 2009. Environmental
biomarkers in white stork (Ciconia ciconia) plasma. SETAC Europe 19th Annual Meeting Protecting Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May4 June, Gothenburg, Sweden.
194. Katia, P, Rema, P, Peixoto, F, Dias, J. 2009. Dietary hemp by-products as alternative dietary
sources in juvenile turbot (Scophtalmus Maximus). XVIII Congress of zootecnic and II
Iberoamerican Congress of Zootecnic, University of Trás-os-Montes and Alto Douro, 6-9 May, Vila
Real, Portugal. Abstarct Book, pp: 560-564. ISBN 978-989-96219-1-6.
195. Keller, T, Costa, R, van Overbeek, L, Jousset, A, Küsel, K, van Elsas, JD. 2009. Diversity and
antagonistic potential of Pseudomonas spp. associated with the freshwater sponge Ephydatia
fluviatilis. 10th Symposium on Bacterial Genetics and Ecology, 15-19 June, Uppsala, Sweden.
196. Kopecka, J., Coimbra, J. 2009. Short term high hydrostatic pressure effect on selected biomarkers
in silver eel (Anguilla anguilla). XIth International Meeting on High Pressure Biology, 31 August-1
September, Brest, France.
197. Kopecka-Pilarczyk, J, Coimbra, J. 2009. Effects of high hydrostatic pressure on selected
biomarkers in silver eel. PRIMO 15 - 15th International Symposium on Pollutant Responses in
Marine Organisms, 17-20 May, Bordeaux, France.
198. Leão, PN, Pereira, AR, Kõnig, GM, Vasconcelos, VM, Gerwick, WH. 2009. New peptidic
allelochemicals from the filamentous cyanobacterium Oscillatoria sp.: isolation and structural
studies. 6th European Conference on Marine Natural Products, 19-23 July, Porto, Portugal.
199. Leão, PN, Pereira, AR, Vasconcelos, VM, Gerwick, WH. 2009. Portoamides - Novos aleloquímicos
produzidos pela cianobactéria de água doce Oscillatoria sp. 1st Iberian Congress in Cyanotoxins,
6-7 July, Porto, Portugal. (Oral communication)
200. Leão, PN, Vasconcelos, MTSD, Vasconcelos, VM. 2009. Allelopathic activity dependence on
growth stage and abiotic factors in the cyanobacterium Oscillatoria sp. SAME 11 - Symposium on
Aquatic Microbial Ecology, 30 August-4 September, Piran, Slovenia.
201. Leite, RB, Afonso, R, Cancela, ML. 2009. Alternative oxidases in Perkinsus sp. Characterization and
evolutionary considerations. XX Molecular Parasitology Meeting, 13-17 September, Woods Hole,
USA.
202. Leite, RB, Bensimon-Brito, A, Cancela, ML. 2009. Hypoxia-inducible factor 1α in gilthead
seabream (Sparus aurata) - a correlation with skeletal structures Interdisciplinary Approaches in
Fish Skeletal Biology, 27-28 April, Tavira, Algarve, Portugal.
203. Lino, PG, Bentes, L, Abecasis, D, Santos, MN, Erzini, K. 2009. Effect of cage acclimation on the
dispersion of two hatchery produced and reared seabreams (Diplodus sargus and D. cervinus) off
the South coast of Portugal. 8th Conference on Fish Telemetry, 14-18 September, Umea,
Sweden.
204. Lino, PG, Bentes, L, Oliveira, MT, Erzini, K, Santos, MN. 2009. The African hind’s (Cephalopholis
taeniops, Serranidae) use of artificial reefs off Sal Island (Cape Verde): a preliminary study based
on acoustic telemetry. 9th International Conference on Artificial Reefs and Related Aquatic
Habitats, 8-13 November, Curitiba, Brazil.
205. Lobo-da-Cunha, A, Ferreira, I, Calado, G. 2009. Histology and ultrastructure of the posterior
oesophagus of Bulla striata (Mollusca). XXIV Congress of the Spanish Microscopy Society - XLIV
Meeting of the Portuguese Society for Microscopy, 16-19 June, Segovia, Spain.
206. Lopes, VR, Gaifem, JF, Silva, CS, Vasconcelos, VM. 2009. Risco e relevância ecológica das
cianobactérias bénticas em estuários. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto,
Portugal. (Oral communication).
207. Lopes, VR, Vasconcelos, VM. 2009. Benthic cyanobacteria induce mutagenicity. SETAC Europe
19th Annual Meeting - Protecting Ecosystem Health: Facing the Challenge of a Globally Changing
Environment, 31st May-4 June, Gothenburg, Sweden.
208. Lopes, VR, Vasconcelos, VM. 2009. Potential SOS - induction in E.coli by benthic estuarine
cyanobacteria. SAME 11 - 11th Symposium on Aquatic Microbial Ecology, 30 August-4
September, Piran, Slovenia.
209. Louro, B, Hadjipavlou, G, Hemani, G, Leach, R, Nadaf, J, Rowe, S, de Koning, DJ. 2009. Extensive
QTL and association analyses of the QTLMAS2009 data. 13th QTL-MAS Workshop, 20-21 April,
Wageningen, The Netherland. (Oral communication)
210. Louro, B, Hellemans, B, Massault, C, Volckaert, FAMJ, Haley, C, de Koning, DJ, Canario, AVM,
Power, DM. 2009. A comparative approach to the identification of growth related QTLs in
European sea bass (Dicentrarchus labrax). European Association for Animal Production Meeting,
24-27 August, Barcelona, Spain.
211. Luís, L, Gravato, C, Guilhermino, L. 2009. Effects of naphthalene in the common prawn
(Palaemon serratus): LC50, biomarkers and avoidance behaviour. SETAC North America 30th
Annual Meeting, Human-Environment Interactions: Understanding Change in Dynamic Systems,
19-23 November, New Orleans, USA.
212. Luís, LG, Gravato, C, Guilhermino, L. 2009. Acute effects of naphtalene on the prawn Palaemon
serratus: mortality and biomarker responses. SETAC Europe 19th Annual Meeting - Protecting
Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May-4 June,
Gothenburg, Sweden.
213. Machado, A, Magalhães, C, Mucha, A, Bordalo, AA. 2009. Abundance and structure of denitrifier
communities and its relationship with denitrification and nitrous oxide production in satmarshes
in temperate estuaries. ASLO Aquatic Sciences Meeting, 25-30 January, Nice, France. Book of
Abstracts, p. 67.
214. Machado, A, Magalhães, C, Mucha, AP, Bordalo, AA. 2009. Abundance, community structure and
nitrous oxide potential rates of denitrifiers associated with rhizosediment and un-colonized
sediments in salt marshes of two Portuguese estuaries. BioMicroWorld - III International
Conference on Environmental, Industrial and Applied Microbiology, 2-4 December, Lisbon,
Portugal.
215. Machado, A, Magalhães, C, Mucha, AP, Bordalo, AA. 2009. Diversity and abundance of bacteria
community associated with rhizosediment and un-colonized sediments in salt marshes of two
Portuguese estuaries. BioMicroWorld - III International Conference on Environmental, Industrial
and Applied Microbiology, 2-4 December, Lisbon, Portugal.
216. Madeira, S, Gonçalves, J, Bastos, L. 2009. Fast camera calibration for low cost mobile mapping.
MMT09 - 6th International Symposium on Mobile Mapping Technology, 21-24 July, São Paulo,
Brazil.
217. Madureira, TV, Barreiro, JC, Rocha, MJ, Rocha, E, Cass, QB, Tiritan, ME. 2009. Monitoring
pharmaceutical drugs in the Douro River estuary, Portugal. EMEC10 - 10th European Meeting on
Environmental Chemistry, 2-5 December, Limoges, France.
218. Madureira, T, Barreto, J, Rocha, MJ, Cass, Q, Tiritan, ME. 2009. Pharmaceutical trace analysis in
aqueous environmental matrices by liquid chromatography-ion trap mass spectrometry. 34th
International Symposium on High Performance Liquid Phase Separations and Related Techniques
HPLC 2009, 28 June-2 July, Dresden, Germany.
219. Magalhães, C, Kiene, RP, Machado, A, Teixeira, C, Bordalo, AA. 2009. DMSP and methionine
degradation exacerbates N2O production: from bacterial cultures to estuarine sediments.
American Society of Limnology and Oceanography - ASLO Aquatic Science Meeting, 25-30
January, Nice, France.
220. Magalhães, C, Matos, P, Machado, A, Bordalo, A. 2009. Impact of copper on denitrification
process and on the microbial communities involved. BioMicroWorld - III International Conference
on Environmental, Industrial and Applied Microbiology, 2-4 December, Lisbon, Portugal.
221. Manoch, L, Dethoup, T, Buaruang, J, Piriyaprin, S, Kijjoa, A. 2009. Antifungal Activities of the
crude extracts of marine sponge-associated against plant pathogenic fungi. 6th European
Conference on Marine Natural Products, 19-23 July, Porto, Portugal.
mechanisms of BMP2. Proceedings of the 34th FEBS Congress, 4-9 July, Prague, Czech Republic.
FEBS Journal 276: 111.
223. Marques, CL, Laizé, V, Cancela, ML. 2009. Regulation of seabream BMP2 gene by bone-related
transcription factors. Interdisciplinary Approaches in Fish Skeletal Biology, 27-29 April, Tavira,
Portugal.
224. Martínez-Páramo, S, Martínez-Pastor, F, Martínez-Rodríguez, G, Herráez, MP, Cabrita, E. 2009.
Antioxidant status in fresh and cryopreserved sperm from gilthead sea bream (Sparus aurata).
Second International Workshop on Biology of Fish Gametes, 9-11 September, Valencia, Spain.
225. Martins, AJ, Moreira, CI, Freitas, MA, Regueiras, A, Antunes, A, Vasconcelos, VM. 2009.
Monitoring Microcystis sp. abundance and toxigenicity by Real-Time PCR in a recreational and
water supply system (north Portugal). SETAC Europe 19th Annual Meeting - Protecting Ecosystem
Health: Facing the Challenge of a Globally Changing Environment, 31 May-4 June, Gothenburg,
Sweden.
226. Martins, AJ, Moreira, CI, Freitas, MA, Regueiras, A, Antunes, A, Vasconcelos, VM. 2009.
Monitorização da abundância relativa de espécies toxicogénicas de Microcystis sp. por PCR em
tempo real. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
227. Martins, GM, Jenkins, SR, Hawkins, SJ, Neto, AI, Thompson, RC. 2009. The exploitation of patellid
limpets in the Azores. International Meeting on Marine Resources, 16-18 November, Peniche,
Portugal.
228. Martins, JC, Leão, P, Vasconcelos, VM. 2009. Differential protein expression in Corbicula fluminea
upon exposure to a Microcystis aeruginosa toxic strain. SETAC Europe 19th Annual Meeting Protecting Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May4 June, Gothenburg, Sweden.
229. Martins, JC, Martins, A, Machado, J, Azevedo, J, Vasconcelos, VM. 2009. Biomarkers gene
expression analysis in the bivalve Corbicula fluminea upon exposure to a Microcystis aeruginosa
toxic strain. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
230. Martins, M, Barros, R, Faleiro, ML, Matos, AP, Costa, MC. 2009. Characterization of a bacterial
consortium with potential for bioremediation of effluents containing uranium. 14th European
Congress on Biotechnology, 13-16 September, Barcelona, Spain.
231. Martins, M, Faleiro, ML, Matos, AP, Costa, MC. 2009. Anaerobic bio-removal of uranium (VI) by a
bacterial community from soil of Monchique Thermal place. XXIV Congress of the Spanish
Microscopy Society - XLIV Meeting of the Portuguese Society for Microscopy, 16-19 June,
Segovia, Spain.
232. Martins, R, Seabra, R, Ramos, V, Tamagnini, P, Vasconcelos, V. 2009. Ecotoxicologia de
cianobactérias marinhas isoladas da costa litoral portuguesa. 1st Iberian Congress in Cyanotoxins,
6-7 July, Porto, Portugal. (Oral communication)
233. Massa, SI, Serrão, EA, Pearson, GA, Duarte, CM, Arnaud-Haond, S. 2009. Temperature tolerance
and the response of distinct genotypes of the seagrass Zostera noltii to heat stress conditions in
Southern Europe. DIVERSITAS OSC2 - Biodiversity and society: understanding connections,
adapting to change, 13-16 October, Cape Town, South Africa.
234. Mata, L. 2009. Tank cultivation of the seaweed species Asparagopsis armata and A. taxiformis
(Bonnemaisoniaceae) using fish pond effluents. 24th ASPAB, 9-12 November, Townsville,
Australia.
235. Matos, E, Gonçalves, A, Dias, J, Dinis, MT. 2009. Effect of harvesting stress and slaughter
conditions on selected flesh quality criteria of gilthead seabream (Sparus aurata). 3rd Joint TransAtlantic Fisheries Technology Conference, 15-18 September, Copenhagen, Denmark.
236. Matos, E, Santos, A, Tiago, T, Aureliano, M, Dinis, MT., Dias, J. 2009. Differential scanning
calorimetry as a tool to assess protein degradation in gilthead seabream (Sparus aurata) fillets:
effect of slaughter stress and storage conditions. International Meeting on Marine Resources, 1618 November, Peniche, Portugal. (Oral communication)
237. Matos, P, Fontaínhas-Fernandes, A, Galante, MH, Monteiro, RAF, Rocha, E. 2009. The use of Nile
tilapia as an experimental model for studying liver necrosis and its functional consequences.
PRIMO 15 - 15th International Symposium on Pollutant Responses in Marine Organisms, 17-20
May, Bordeaux, France.
238. Mendes, JS, Moreira, C, Vasconcelos, VM. 2009. Monitoring and toxicity ealuation of
cyanobacteria from Palácio de Cristal lakes by applying molecular methodologies. 1st Iberian
Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
239. Mesquita, A, Avilez-Valente, P, Pinho, FT. 2009. Turbulência e rebentação sobre quebramares
submerses. Proceedings. of the METNUM 2009 - Métodos Numéricos en Ingeniería 2009. Huerta,
A, Oñate, E, Rodríguez Ferran, A, Figueiredo, IN, Menezes, LF, Tadeu, AJB (Eds), 29 June-2 July,
Barcelona, Spain, 20 pages, CD-ROM. ISBN 78-84-96736-66-5. (Oral communication)
240. Mesquita, A, Avilez-Valente, P. 2009. Numerical depth inversion of the entrance of Leixões
harbor. Proceedings. of the Twelfth International Conference on Civil, Structural and
Environmental Engineering Computing - CC2009. Topping, BHV, Costa Neves, LF, Barros, RC (Eds),
1-4 September, Funchal, Portugal, Paper 248, 16 pp., CD-ROM, Civil-Comp Press, Stirlingshire, UK.
ISBN 978-1-905088-32-4. (Oral communication)
241. Mohseni, M, Pourali, H, Pourkazemi, M., Ozorio, ROA, Bai SC. 2009. Optimal dietary protein
requirements of juvenile and su yearling beluga (Huso huso). 6th International Symposium on
Sturgeon, Endangered Giants - Living Fossils: Human impacts on sturgeons and conservation
measures, 25 -30 October, Wuhan, China.
242. Monteiro, CM, Eiras, JC, Brasil-Sato, M. 2009. A new species of Myxobolus (Myxozoa,
Myxosporea, Myxobolidae) from the upper São Francisco River, Brazil. 40th Annual Meeting of
the Rock Mountain Conference of Parasitologists, 10-12 September, Moran, Wyoming. Award M.
Hammond (best poster).
243. Moraes, JRE, Iwashita, MK, Moraes, FR, Ozório, ROA, Nakandakare, IB. 2009. The influence of the
vitamin E in the kinetics of the wound healing process induced in Nile tilapias Oreochromis
niloticus. World Aquaculture 2009, 25-29 May, Vera Cruz, Mexico.
244. Morais, S, Alves Martins, D, Castanheira, F, Mendes, A, Coutinho, J, Bandarra, NM, PousãoFerreira, P, Conceição, LEC. 2009. A new method for the study of essential fatty acid
requirements in fish larvae. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium,
7-10 September, Ghent, Belgium. European Aquaculture Society, Hendry, CI, Van Stappen, G,
Wille, M, Sorgeloos, P (Eds), Special Publication nº38: 279-281.
245. Moreira, C, Martins, A, Antunes, A, Vasconcelos, V. 2009. Application of real-time PCR in
monitoring Cylindrospermopsis raciborskii in Portuguese freshwaters: abundance and
toxicological evaluation. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health:
Facing the Challenge of a Globally Changing Environment, 31 May-4 June 2009, Gothenburg,
Sweden. (Oral communication)
246. Moreira, C, Martins, A, Vasconcelos, VM. 2009. Cianobactérias e suas toxinas no arquipélago dos
Açores e México. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
247. Moreira, C, Vasconcelos, V, Antunes, A. 2009. Diversidade genética e estrutura populacional de
Microcystis aeruginosa em Portugal. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto,
Portugal.
248. Moreira, C, Vasconcelos, V, Antunes, A. 2009. Phylogenetic studies in cyanobacteria. XII Foro dos
Recursos Marinos e da Acuicultura das Rías Galegas, 8-9 October, Vigo, Spain.
249. Moreira, C, Vasconcelos, V, Antunes, A. 2009. Genetic diversity and geographical distribution of
the invasive cyanobacterium Cylindrospermopsis raciborskii. BIOLIEF - World Conference on
Biological Invasions and Ecossystem Functioning, 27-30 October, Porto, Portugal.
250. Moreira, SM, Moreira-Santos, M, Rendon von-Osten, J, da Silva, EM, Ribeiro, R, Guilhermino, L,
Soares, AMVM. 2009. Ecotoxicological tools for the tropics: sub-lethal assays with fish to
evaluate the toxicity of edge-of-field pesticide runoff. SETAC North America 30th Annual
Meeting, Human-Environment Interactions: Understanding Change in Dynamic Systems, 19-23
November, New Orleans, USA.
251. Mota, M, Antunes, C, Costa-Dias, S, Sousa, R. 2009. Temporal changes of the icthyofauna of the
Minho River estuary: the importance of non-indigenous species. World Conference on Biological
Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal. Book of Abstracts, p. 42.
252. Mota, M, Antunes, C. 2009. First characterization of the status of Allis shad (Alosa alosa)
populations in the Minho River, NW of the Iberian Peninsula - a recovering population?
International Workshop on the Restoration of Fish Populations, 1-4 September, Düsseldorf,
Germany.
253. Mucha, AP, Almeida, CMR, Bordalo, A, Vasconcelos, MTSD. 2009. Exudation of organic acids by
two salt marsh plants: Juncus maritimus and Scirpus maritimus. BIOGEOMON 2009 - 6th
International Symposium on Ecossystem Behavior, 29 June-3 July, Helsinky, Filand.
254. Mucha, AP, Almeida, CMR, Magalhães, CM, Vasconcelos, MTSD, Bordalo, A. 2009. Interactions
microorganism-salt marsh plants in the presence of Cu and PAHs contamination. BioMicroWorld
- III International Conference on Environmental, Industrial and Applied Microbiology, 2-4
December, Lisbon, Portugal. (Oral communication)
255. Neves, J, Wilson, JM, Rodrigues, P. 2009. Characterization and expression analysis of immunerelated iron genes during experimental iron overload and infection in fish. International BioIron
Society 2009 Meeting, 7-11 June, Porto, Portugal. (Oral communication)
256. Neves, J, Wilson, JM, Rodrigues, P. 2009. Response of iron-related immune genes during
experimental infection in fish. 11th International Congress of the ISDCI, 28 June-4 July, Prague,
Czech Republic. (Oral communication)
257. Nogueira, ICG, Lobo-da-Cunha, A, Rivera, S, Azevedo, J, Monteiro, R, Cervantes, R, GagoMartinez, A, Vasconcelos, V. 2009. Neurotoxic effects of domoic acid in the seabream Sparus
aurata. X Iberian Meeting of Toxic Phytoplankton and Biotoxins. 13 May, Lisbon, Portugal. (Oral
communication).
258. Novais, S, Gomes, S, Gravato, C, Amorim, M, Guilhermino, L, Soares, AMVM, De Coen, W. 2009.
Responses in Enchytraeus albidus (Oligochaeta) exposed to heavy-metals: effects at different
levels of biological organization. Interdisciplinary Symposium on Biological Responses to
Chemical Contaminants: from Molecular to Community Level, 2-4 September, Aveiro, Portugal.
259. Novais, S, Gravato, C, Amorim, M, Guilhermino, L, Soares, A, De Coen, W. 2009. Ecological
relevance of oxidative stress biomarkers in Enchytraeus albidus (Oligochaeta): effects of zinc and
cadmium. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
Challenge of a Globally Changing Environment, 31 May-4 June, Gothenburg, Sweden. (Oral
communication)
260. Oliva, M, Vicente, JJ, Galindo-Riano, MD, Gravato, C, Guilhermino, L, Canales, MLG. 2009.
Oxidative stress biomarkers in Senegal sole (Solea senegalensis) from a Huelva estuary (Cadiz
Gulf, SW Spain). SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
261. Oliva-Teles, A. 2009. Nutritional strategies for the health and welfare of aquaculture fish.
Proceedings of the 3º Simpósio Internacional de Nutrição e Saúde de Peixes, 4-6 November,
Botucatu, São Paulo, Brazil, pp. 80-99.
262. Oliveira, C, Gravato, C, Soares, A, Guilhermino, L. 2009. Effects of pesticides on common prawn
behaviour (Palaemon serratus): a new avoidance test. SETAC Europe 19th Annual Meeting Protecting Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May4 June, Gothenburg, Sweden.
263. Oliveira, C, Gravato, C, Soares, AMVM, Guilhermino, L. 2009. Effects of fenitrothion on
biomarkers and behaviour of the common prawn (Palaemon serratus). SETAC North America
30th Annual Meeting, Human-Environment Interactions: Understanding Change in Dynamic
Systems, 19-23 November, New Orleans, USA.
264. Oliveira, P, Almeida, R, Gravato, C, Guilhermino, L. 2009. Biomonitoring study using mussels from
the NW portuguese coast in relation to environmental contamination by polycyclic aromatic
hydrocarbons. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
265. Oliveira, P, Gravato, C, Guimarães, L, Guilhermino, L. 2009. Accumulation of PAHs in Mytilus
galloprovincialis and their relation with biomarkers: a monthly biomonitoring study in the NW
coast of Portugal. SETAC North America 30th Annual Meeting, Human-Environment Interactions:
Understanding Change in Dynamic Systems, 19-23 November, New Orleans, USA.
266. Oropesa, AL, Soler Rodríguez, F, Zalba, J, Gravato, C, Guilhermino, L. 2009. Defensas antioxidantes de origen enzimático en cigüeña blanca (Ciconia ciconia): aplicación para
biomonitorización de la contaminación por agentes que originan fenómenos de estrés oxidativo.
XVIII Spanish Congress of Toxicology, 9-11 de September, Palma, Mallorca.
267. Oropesa, AL, Soler, F, Pérez-Lopez, M, Zalba, J, Gravato, C, Guilhermino, L. 2009. Characterization
of cholinesterase in plasma from white stork (Ciconia ciconia) and its application like an
environmental biomarker of effect. ISTA 14 - 14th International Symposium on Toxicity
Assessment, 30 August-4 September, Metz, France.
268. Oropesa, AL, Soler, F, Pérez-Lopez, M, Zalba, J, Gravato, C, Guilhermino, L. 2009. In vitro
inhibition of cholinesterase in plasma from white stork (Ciconia ciconia) by mixture of
anticholinesterase agents. ISTA 14 - 14th International Symposium on Toxicity Assessment, 30
August-4 September, Metz, France.
269. Osswald, J, Azevedo, J, Vasconcelos, V, Guilhermino, L. 2009. Degradation of the cyanobacterial
toxin anatoxin-a, in water under laboratory conditions. SETAC Europe 19th Annual Meeting Protecting Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May4 June, Gothenburg, Sweden.
270. Osswald, J, Passo, J, Guilhermino, L, Vasconcelos, V. 2009. Quantificação da transferência de
anatoxina-a da água para a truta Arco-Íris (Onchorinchus mykiss), em condições laboratoriais. 1st
Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal. (Oral communication)
271. Ozório, ROA, Valenti, WC. 2009. Current status and future perspectives of the Brazilian
aquaculture sector. Proceedings of XVIII Congresso de Zootecnia and II Congresso IberoAmericano de Zootecnia, 6-9 May, Vila Real, Portugal, pp.546-553. (Oral communication)
272. Paiva, L, Patarra, RF, Neto, AI, Lima, EM, Baptista, J. 2009. Comparison of free radical scavenging
activities of selected Azorean seaweeds. 7th Asia-Pacific Conference on Algal Biotechnology, 1-4
December, New Delhi, India.
273. Parameswaran, V, Laizé, V, Cancela, ML. 2009. ESSA1 embryonic stem cells: A new tool to study
osteoblast, osteoclast and chondrocyte differentiation in gilthead seabream. Interdisciplinary
274. Páscoa, I, Fontaínhas-Fernandes, A, Castro, LFC, Wilson, JM. 2009. Regulação hormonal da
expressão de transportadores de amónia na brânquia de peixe zebra. In: Avances en
Endocrinología Comparada. 7th AIEC Congress – Iberian Association of Comparative
Endocrinology, 6-9 September, Porto, Portugal.
275. Pascoal, J, Power, DM, Cardoso, JCR. 2009. Functional characterisation of a novel PTH/PTHrP
receptor in tetrapods. 7th AIEC Congress – Iberian Association of Comparative Endocrinology, 6-9
276. Passo, J, Osswald, J, Guilhermino, L, Vasconcelos, V. 2009. Desenvolvimento e validação de um
método de HPLC-FLD para a determinação da anatoxina-a na truta Arco-Íris (Onchorinchus
mykiss), em condições laboratoriais. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto,
Portugal. (Oral communication)
277. Patarra, RF, Paiva, L, Neto, AI, Lima, EM, Baptista, J. 2009. Protein and fiber of selected Azorean
seaweeds. 7th Asia-Pacific Conference on Algal Biotechnology, 1-4 December, New Delhi, India.
278. Peña, V, Bárbara, I, Berecibar, E, Santos, R. 2009. Present distribution of maerl beds in the
atlantic Iberian Peninsula. International Fossil Algae Association - 6th Regional Symposium, 1-5
July, Milan, Italy.
279. Pereira, AL, Martins, MC, Oliveira, M, Carrapiço, F. 2009. Assessing the morphological and genetic
diversity of Family Azollaceae. Proceedings of the XVII Simpósio de Botânica Criptogâmica, 23-26
September, Tomar, Portugal, pp. 225-226. (Oral communication)
280. Pereira, M, Capela, RC, Sarria, M, Monteiro, NM, Correia, AT. 2009. New insights about the lifehistory of two syngnathids species (Syngnathus abaster and Nerophis lumbriciformis) inferred
from the microstructure and microchemistry of otoliths. 44th European Marine Biology
Symposium, 7-11 September, Liverpool, England.
281. Pereira, R, Abreu, H, Araújo, R, Carvalho, F, Machado, L, Vilela, J, Silva, F, Pinheiro, I, Sousa-Pinto,
I. 2009. Influence of environmental parameters in early stages of development of the introduced
species Grateloupia turuturu. BIOLIEF - World Conference on Biological Invasions and Ecosystem
Functioning, 27-30 October, Porto, Portugal.
282. Pereira, SR, Vasconcelos, VM, Antunes, A. 2009. A dinâmica evolucionária das dosfatases de
proteína PPP nos metazoários. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
283. Pereira, SR, Vasconcelos, VM, Antunes, A. 2009. The evolutionary dynamics of the catalytic
subunits of phosphoprotein phosphatases in the metazoan. EuroPhosphatases 2009 - Protein
Phosphatases in Development and Disease, 14-18 July, Egmond aan Zee, The Netherlands.
284. Peres dos Santos, R, Bensimon-Brito, A, Gavaia, P, Cancela, ML. 2009. Warfarin effects in the
skeletal development of zebrafish (Danio rerio). Interdisciplinary Approaches in Fish Skeletal
285. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2009. Sensitivity of trout sperm
to cryopreservation during the reproductive season. Second International Workshop on Biology
of Fish Gametes, 9-11 September, Valencia, Spain.
286. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2009. Sensitivity DNA damage in
fish sperm cryopreservation. Second International Workshop on Biology of Fish Gametes, 9-11
September, Valencia, Spain. (Oral communication).
histopathological and molecular data. British Society of Parasitology Spring & Malaria Meeting, 58 April, Edinburgh, Scotland.
288. Pina, S, Tajdari, J, Russell-Pinto, F, Rodrigues, P. 2009. Morphological and molecular studies on
life cycle stages of Diphtherostomum brusinae (Digenea: Zoogonidae) from northern Portugal.
British Society of Parasitology Spring & Malaria Meeting, 5-8 April, Edinburgh, Scotland.
289. Pinheiro, P, Downie, H, Fuentes, J, Power, DM, Canario, AVM. 2009. Functional activity and gene
expression of parathormone. IUPS - XXXVIth International Union of Physiological Sciences, 27
July-1 August, Kyoto, Japan. The Journal of Physiological Sciences 59 (Supp 1): 273.
290. Pinheiro, P, Fuentes, J, Power, DM, Canário, AVM. 2009. The parathormone and related peptides
activity and expression in chicken. 7th AIEC Congress – Iberian Association of Comparative
291. Pinto, F, Olabarria, C, Sousa-Pinto, I, Arenas, F. 2009. Interactive effects of functional diversity
and propagule limitation at different invasion stages. BIOLIEF - World Conference on Biological
Invasion and Ecosystem Functioning, 27-30 October, Porto, Portugal.
292. Pinto, F, Wallenstein, FM, Álvaro, NV, Neto, AI. 2009. Description of intertidal algal-based
biotopes of Pico Island, Azores, Portugal. ISMS09 - II International Symposium of Marine Science,
27-30 April, Vigo, Spain. Abstract Book, pp. 141-142.
293. Pinto, PIS, Power, DM, Canario, AVM, Thorne, MAS, Reinhardt, R, Matsumura, H, Terauchi, R.
2009. Transcriptome profiling of the gills of a euryhaline teleost fish, Tetraodon nigroviridis, in
response to altered calcium concentrations in water. ESCBP – Proceedings of the 26th Congress
of the European Society of Comparative Biochemistry and Physiology, 6-10 September,
Innsbruck, Austria. Comparative Biochemistry and Physiology A - Molecular & Integrative
Physiology 154(1): S2-S3. (Oral communication)
294. Pinto, W, Figueira, L, Rodrigues, V, Dinis, MT, Aragão, C. 2009. The importance of aromatic amino
acids during fish metamorphosis. Proceedings of the Larvi’09 - Fish & Shellfish Larviculture
Symposium, 7-10 September, Ghent, Belgium. European Aquaculture Society, Hendry, CI, Van
Stappen, G, Wille, M, Sorgeloos, P (Eds). Special Publication nº38: 329-332.
295. Poinar, G, Duarte, D, Santos, MJ. 2009. A new parasitic species of Talorchestia brito (Crustacea:
Talitridae) in Portugal: Halomonhystera parasitica Poinar, Duarte and Santos, in press
(Nematoda: Monhysteridae). XI Iberian Congress of Parasitology, 15-18 September, Lisbon,
296. Prabhu, CN, Abrantes, F. 2009. Holocene climate influence on South Asian cultural evolution.
PAGES 3rd Open Science Meeting, 8-11 July, Oregon State University, Corvallis, USA.
297. Quartau, R. 2009. Integration and synthesis of MARGINS sediment source-to-sink research.
Towards Integration and Synthesis of MARGINS S2S Research in PNG and NZ Focus Areas 5-9
April, Gisborne, New Zealand.
298. Quartau, R. 2009. The Fail Island Shelf. VI International Workshop Palaeontology in Atlantic
Islands, 19-28 June, Sta. Maria, Azores, Portugal.
299. Rábade, T, Gravato, C, Fernández-Tajes, J, Laffon, B, Méndez, J, Guilhermino, L. 2009. Use of
glutathione S-transferases and cholinesterase activities as biomarkers of environmental
contamination in Mytilus galloprovincialis. SETAC Europe 19th Annual Meeting - Protecting
Ecosystem Health: Facing the Challenge of a Globally Changing Environment, 31 May-4 June,
Gothenburg, Sweden.
300. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2009. Bone-related role of the LIM-only protein FHL2
in fish. Proceedings of the 36th ECTS Meeting, 23-27 May, Vienna, Austria. Bone 44: S311-S312
301. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2009. Seabream as an alternative vertebrate model
to investigate the role of FHL2 during skeletogenesis. Interdisciplinary Approaches in Fish Skeletal
302. Ramalho, A, Conceição, LEC, Dias, J, Soares, F, Valente, L, Vaz-Pires, P, Dinis, MT. 2009.
Sustentabilidade da Aquacultura semi-intensiva no sul da Europa: projecto SEACASE. XVIII
Congresso de Zootecnia and II Congresso Ibero-Americano de Zootecnia, 6-9 May, Vila Real,
Portugal. (Invited Oral communication).
303. Ramos, V, Seabra, R, Brito, A, Santos, A, Lopo, M, Santos, C, Moradas-Ferreira, P, Vasconcelos, V,
Tamagnini, P. 2009. An unusual unicellular cyanobacterium isolated from intertidal rocks from
the south of Portugal and its relation to thermophilic strains. 3rd Congress of European
Microbiologists – FEMS, 28 June-2 July, Gothenburg, Sweden.
304. Ramos, V, Seabra, R, Brito, A, Santos, A, Lopo, M, Martins, R, Moradas-Ferreira, P, Vasconcelos,
V, Tamagnini, P. 2009. Biodiversity of cyanobacteria on the Portuguese coast. 3rd Congress of
European Microbiologists - FEMS, 28 June-2 July, Gothenburg, Sweden.
305. Range, P, Chícharo, L, Chícharo, MA, Ben-Hamadou, R, Piló, D, Matias, D, Joaquim, S, Oliveira, AP.
2009. Effects of ocean acidification on juvenile clams Ruditapes decussatus. 39th CIESM
Congress, 10-14 May, Venice, Italy.
306. Rangel, MO, Gonçalves, JMS, Leite, L, Erzini, K. 2009. Implementation of eco-tourism underwater
routes in the Algarve (South of Portugal) as a way of preserving pristine marine ecosystems.
International Meeting on Marine Resources, 16-18 November, Peniche, Portugal.
307. Rêgo, PC, Afonso, A, Santos, N, Rocha, E. 2009. Interhepatocytic non-pigmented macrophage-like
cells in the liver of brown trout. 11th Congress of the International Society of Developmental and
Comparative Immunology, 28 June-3 July, Prague, Czech Republic. (Oral communication)
308. Regueiras, A, Freitas, MA, Martins, AJ, Moreira, CI, Antunes, A, Vasconcelos, VM. 2009.
Optimização do volume de amostragem para monitorização de cianobactérias e cianotoxinas por
métodos moleculares e imunológicos. 1st Iberian Congress in Cyanotoxins, 6-7 July, Porto,
Portugal.
309. Regueiras, A, Freitas, MA, Martins, AJ, Moreira, CI, Vasconcelos, VM. 2009. Monitoring toxic
cyanobacteria and their toxins in Portuguese freshwaters using immunoassays and molecular
techniques. SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the
310. Rendón von-Osten, J, Ortíz, A, Memije, M, Mirandas-Rosas, G, Guilhermino, L. 2009. Pink shrimp
biomonitoring study in open sea in relation to off-shore oil platforms and other anthropogenic
activities: a case study from Mexico. SETAC North America 30th Annual Meeting, HumanEnvironment Interactions: Understanding Change in Dynamic Systems, 19-23 November, New
Orleans, USA.
311. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Monteiro, RAF, Rocha, E. 2009. Morphofunctional
correlations in brown trout (Salmo trutta) kidney peroxisomes. XXIV Congress of the Spanish
Microscopy Society - XLIV Meeting of the Portuguese Society for Microscopy, 16-19 June,
Segovia, Spain.
312. Ribeiro, C, Urbatzka, R, Castro, LFC, Rocha, E, Carrola, J, Fontainhas-Fernandes, A, Rocha, MJ.
2009. Changes in mRNA expression of genes encoding steroidogenic enzymes in testes from Nile
tilapia exposed to estrogen endocrine disruptors in vivo. PRIMO 15 - 15th International
Symposium on Pollutant Responses in Marine Organisms, 17-20 May, Bordeaux, France.
313. Ribeiro, H, Mucha, AP, Almeida, CMR, Bordalo, A. 2009. Influence of plant-microorganisms
associations on hydrocarbons degradation in salt marshes. WETPOL 2009 - 3rd Wetland Pollutant
Dynamics and Control Symposium, 20-24 September, Barcelona, Spain.
314. Ribeiro, H, Mucha, AP, Almeida, CMR, Bordalo, A. 2009. Plant-microorganisms associations in salt
marshes: influence on hydrocarbon degradation. BioMicroWorld - III International Conference on
Environmental, Industrial and Applied Microbiology, 2-4 December, Lisbon, Portugal.
315. Ribeiro, R, Hubbert, F, Rojas-García, C, Rønnestad, I, Engrola, S, Dinis, MT, Power, D. 2009. Cofeeding in Senegalese sole at mouth opening: Consequences on digestive physiology.
Proceedings of the Larvi’09 - Fish & Shellfish Larviculture Symposium, 7-10 September, Ghent,
Belgium. European Aquaculture Society, Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P (Eds),
Special Publication nº38: 362-365.
316. Richard, N, Gavaia, PJ, Cordeiro, O, Silva, TS, Rodrigues, C, Rodrigues, PM, Conceição, LEC. 2009.
Vitamin K supplementation in the diet affects the proteome and skeletal development of
Senegalese sole (Solea senegalensis). Workshop on Interdisciplinary Approaches in Fish Skeletal
317. Roberto, V, Cavaco, S, Simes, DC, Gavaia, PJ, Cancela, ML. 2009. Mgp expression and
accumulation in heart and kidney of turbot (Scophthalmus maximus). Interdisciplinary
318. Rocha, AC, Almeida, CMR, Vasconcelos, MTSD. 2009. Influence of Cu, Ni and Cd on the exudation
of organic acids by Halimione portulacoides in a hydroponics medium. BIOGEOMON 2009 - 6th
International Symposium on Ecosystem Behaviour, 29 June-3 July, Helsinki, Finland.
319. Rocha, C, Redruello, B, Canário, AVM, Power, DM. 2009. Gene expression of parathyroid
hormone receptor 1 and structural extracellular matrix components during sea bream fin
regeneration. 7th AIEC Congress – Iberian Association of Comparative Endocrinology, 6-9
320. Rocha, F, Alves Martins, D, Castanheira, F, Morais, S, Coutinho, J, Bandarra, N, Conceição, L.
2009. Dietary arachidonic acid requirements for gilthead sea bream (Sparus aurata) larvae.
IMMR09 - International Meeting on Marine Resources, 16-18 November, Peniche, Portugal.
321. Rodrigues, P, Silva, T, Jessen, F, Dias, J. 2009. On the reproducibility of a fractionation procedure
for fish muscle proteomics. 9th Internernational Symposium on Mass Spectrometry in the Health
and Life Sciences, 23-27 August, San Francisco, USA.
322. Rodrigues, PM, Cordeiro, O, Silva, TS, Conceição, LEC. 2009. Skeletal development analysis by
two-dimensional electrophoresis (2-DE) in white bream (Diplodus sargus), fed with different diets
(updated). 3rd EuPA Congress, 14-17 June, Stockholm, Sweden.
323. Rodrigues, PM, Silva, TS, Cordeiro, O, Conceição, LEC. 2009. Proteomic characterization of reared
white seabream skeletal deformities using two-dimensional gel electrophoresis: a comparative
study. 3rd EuPA Congress, 14-17 June, Stockholm, Sweden.
324. Rodrigues, T, Voelker, A, Grimalt, JO, Abrantes, F. 2009. Climate off Portugal during Marine
Isotope Stages 15 - 9 (570 to 300 Ka): Suborbital glacial variability and interglacial stability.
American Geosciences Union General Fall Meeting, 14-18 December, San Francisco, USA.
Abstract PP31C-1370.
325. Rodrigues, V, Ribeiro, L Dinis, MT, Dias, J. 2009. Nutritional modulation of innate immune
parameters in the epidermal mucus of Senegalese sole (Solea senegalensis). IMMR09 International Meeting on Marine Resources, 16-18 November, Peniche, Portugal.
326. Rodríguez Benítez, S, Vaz Pinto, F, Sousa Pinto, I, Arenas, F. 2009. Functional consequences of
diversity on macroalgal productivity: disentangling the effects of number, identity and density.
ISMS09 - II International Symposium of Marine Science, 27-30 April, Vigo, Spain.
327. Rosa, J, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2009. Stimulation of bone-derived cell
proliferation and mineralization by fish serum. Interdisciplinary Approaches in Fish Skeletal
328. Saavedra, M, Pousão-Ferreira, P, Barr, Y, Helland, S, Yúfera, M, Dinis, MT, Conceição, LEC. 2009.
Amino acids have important roles in larval development other than growth. Proceedings of the
Larvi’09 - Fish & Shellfish Larviculture Symposium, 7-10 September, Ghent, Belgium. European
Aquaculture Society, Hendry, CI, Van Stappen, G, Wille, M, Sorgeloos, P (Eds), Special Publication
nº38.
329. Salgueiro, E, Martin, PA, Voelker, A, Abrantes, F. 2009. Temperature calibration along the
Iberian-NW Africa margin, using modern planktonic foraminifera trace element and stable
isotope ratios. American Geosciences Union General Assembly, 14-18 December, San Francisco,
USA. Abstract PP31B-1334.
330. Salgueiro, E, Voelker, A, Rodrigues, T, Prabhu, CN, Abrantes, F, Grimalt, JO. 2009. Temperature
and nutrients changes during MIS 11c and the Holocene on the Portuguese margin. Goldschmidt
Conference, 21-26 June, Davos, Switzerland.
331. Santos, M, Campos, A, Carvajal-Vallejos, P, Villalobos, E, Franco, CF, Almeida, AM, Coelho, AV,
Torné, JM. 2009. Characterization of Zea mays L. plastidial transglutaminase: interactions with
thylakoid membrane proteins. 5th Congress of the Portuguese Proteomics Network - Procura and
1st International Congress on Analytical Proteomics - ICAP, 30 September-3 October, Lisbon,
Portugal.
332. Santos, M, Feist, SW, Stentiford, GD, Monteiro, RAF, Rocha, E. 2009. Application of stereological
tools to the characterization of preneoplastic and neoplastic liver lesions if fish - Preliminary data
from the marine flatfish dab. PRIMO 15 - 15th International Symposium on Pollutant Responses
in Marine Organisms, 17-20 May, Bordeaux, France.
333. Santos, MJ. 2009. Parasites as biological tags. Round table: Parasitology and pathology of aquatic
fauna. XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal. (Oral
communication)
334. Santos, P, Barroco, A. 2009. Estratégia Nacional para a Gestão Integrada da Zona Costeira
Portuguesa: a integração das politicas de conservação do património natural. V Congresso sobre
Planeamento e Gestão das Zonas Costeiras dos Países de Expressão Portuguesa, 30 September-2
October, Florianopolis, Brazil.
335. Santos, R, Silva, J. 2009. Spartina maritima community production in southern Europe. CERF Coastal and Estuarine Research Federation 20th Biennial Conference, 1-5 November, Portland,
USA.
336. Saraiva, A. 2009. Nematoda Philometridae. Round table: Parasitology and pathology of aquatic
fauna. XI Iberian Congress of Parasitology, 15-18 September, Lisbon, Portugal. (Oral
communication)
337. Sárria, MP, Santos, MM, Reis-Henriques, MA, Vieira, MN, Monteiro, NM. 2009. Newly born
pipefish short term exposure to mixtures of estrogenic and androgenic endocrine disruptors. 7th
AIEC Congress – Iberian Association of Comparative Endocrinology, 6-9 September, Porto,
Portugal.
338. Segundo, R, Mesquita, R, Ferreira, T, Teixeira, C, Bordalo, AA, Rangel, AOSS. 2009. Sequential
injection system for the spectrophotometric determination of ammonium in Portuguese
estuarine waters. Flow Analysis XI, 14-18 September, Pollensa, Mallorca, Spain.
339. Seixas, P, Otero, A, Aragão, C, Valente, L, Rey Méndez, M. 2009. Efectos sobre el crecimiento de
paralarvas de pulpo (Octopus vulgaris Cuvier, 1797) alimentadas con juveniles de Artemia
suplementados con aminoácidos libres. XII Congreso Nacional de Acuicultura, 24-26 November,
Madrid, Spain.
340. Seixas, P, Rey Méndez, M, Valente, LMP, Otero, A. 2009. High dietary protein:lipid ratio improves
growth of Octopus vulgaris paralarvae. Larvi 2009 - 5th Fish & Shellfish Larviculture Symposium,
7-10 September, Ghent, Belgium. Abstract book, p. 505.
341. Serra, IA, Innocenti, AM, Di Maida, G, Calvo, S, Migliaccio, M, Zambianchi, E, Pizzigalli, C, ArnaudHaond, S, Duarte, CM, Serrão, EA, Procaccini, G. 2009. Genetic discontinuities within the
Mediterranean basin. Post-glacial recolonization patterns and recent sea-currents regime in
Posidonia oceanica L. Delile. Mediterranean Seagrass Workshop, 6-10 September, 2009, Hvar,
Croatia. (Oral communication)
342. Sharon, Y, Silva, J, Santos, R, Runcie, J, Chernihovsky, M, Beer, S. 2009. Photosynthetic responses
of Halophila stipulacea to a light gradient: Acclimation following transplantation. Mediterranean
Seagrass Workshop, 6-10 September, Hvar, Croatia.
343. Silva, CS, Gaifem, JF, Lopes, VR, Vasconcelos, VM. 2009. Avaliação da toxicidade de
cianobactérias estuarinas com ensaios de náuplios de Artemia sp. e microalgas. 1st Iberian
Congress in Cyanotoxins, 6-7 July, Porto, Portugal.
344. Silva, J, Barrote, I, Santos, R. 2009. Oxidative stress and quantum use efficiency in the intertidal
seagrass Zostera noltii. CERF - Coastal and Estuarine Research Federation, 20th Biennial
Conference, 1-5 November, Portland, USA.
345. Silva, J, Barrote, I, Santos, R. 2009. Quantum use efficiency by the intertidal seagrass Zostera
noltii. Mediterranean Seagrass Workshop, 6-10 September, Hvar, Croatia.
346. Silva, JMG, Espe, M, Conceição, LEC, Dias, J, Valente, LMP. 2009. Senegalese sole juveniles Solea
senegalensis (Kaup, 1858) grow equally well on diets devoid of fish meal provided the dietary
amino acids are balanced. Aquaculture Europe 09, 14-17 August, Trondheim, Norway. (Oral
communication)
347. Silva, T, Dias, J, Cordeiro, O, Matos, E, Wulff, T, Jessen, F, Rodrigues, P. 2009. Effect of harvesting
stress on post-mortem changes in the sarcoplasmic proteomic profile of gilthead seabream
(Sparus aurata) muscle. 3rd Joint Trans-Atlantic Fisheries Technology Conference, 15-18
September, Copenhagen, Denmark.
348. Silva, TS, Jessen, F, Cordeiro, O, Dias, J, Rodrigues, PM. 2009. On the reproducibility of a
fractionation procedure for fish muscle proteomics. 9th International Symposium on Mass
Spectrometry in Health and Life Sciences, 23-27 August, San Francisco, California, USA.
349. Simões, B, Conceição, N, Cancela, ML, Kelsh, R. 2009. Investigation of runx3 expression pattern
and function in zebrafish peripheral nervous system development. 6th European Zebrafish
Genetics and Development Meeting, 15-19 July, Rome, Italy.
350. Simões, R, Poirel, L, Martins da Costa, P, Nordmann, P. 2009. Gaivotas como reservatório e vector
de Escherichia coli produtoras de ß-lactamases de espectro alargado. VI SPEA Congress of
Ornithology and IV Iberian Congress of Ornithology, 5-8 December, Elvas, Portugal.
351. Simões, R, Poirel, L, Martins da Costa, P, Nordmann, P. 2009. Seagulls as reservoirs and vehicles
of emerging ESBL determinants. ARAE 2009 - 3rd Symposium on Antimicrobial Resistance in
Animals and the Environment, 1-3 June, Paris, France.
352. Soares, J, Coimbra, AM, Reis-Henriques, MA, Monteiro, NM, Vieira, MN, Oliveira, JMA, GuedesDias, P, Fontaínhas-Fernandes, A, Silva Parra, S, Carvalho, AP, Castro, FC, Santos, MM. 2009.
exposure to low levels of ethinylestradiol. PRIMO 15 - 15th. International Symposium on
Pollutant Responses in Marine Organisms, 17-20 May, Bordéus, France.
353. Sobrinho-Gonçalves, L, Oliveira, P, Catarino, R, Silva, AJ, Bastos, L, Moita, T. 2009. Indicadores
fitoplanctónicos de diferentes massas de água durante o Inverno na costa NO Ibérica - NICC
2006-07. Workshop: Douro e Minho – Estuaries and Coastal Zones, 16-17 April, APDL,
Matosinhos, Portugal. (Oral Communication)
354. Sousa Pinto, I, Vieira, R. 2009. Monitoring intertidal biodiversity with schools. Diversitas OSC2 –
Biodiversity and Society, Understanding Connections, Adapting to Change, 13-16 October, Cape
Town, South Africa.
355. Sousa, I, Coelho, R, Abecasis, R, Gonçalves, JMS, Erzini, K. 2009. Luiz Saldanha Marine Park:
community composition in relation to the protection level and substrate. International Meeting
on Marine Resources, 16-18 November, Peniche, Portugal.
356. Sousa, J, Hooper, A, Hanssen, R, Bastos, L. 2009. Comparative study of two different PS-InSAR
approaches: DePSI vs. StaMPS. Fringe 2009 Workshop, ESA ESRIN, 30 November-4 December,
Frascati, Italy.
357. Sousa, R. 2009. Impacts of extreme climatic events in aquatic ecosystems. Student Conference on
Conservation Science 2009, Cambridge, UK. (Oral communication)
358. Sousa, R, Freitas, F, Illari, M, Costa-Dias, S, Antunes, C, Guilhermino, L. 2009. Corbicula fluminea
as ecosystem engineer: effects on macrozoobenthic assemblages. BIOLIEF - World Conference on
Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal. Book of
Abstracts, p. 113.
359. Sousa, R, Pilotto, F, Aldridge, DC. 2009. Fouling rates of Dreissena polymorpha on European
freshwater mussels: functional importance. BIOLIEF - World Conference in Biological Invasions
and Ecosystem Functioning, 27-30 October, Porto, Portugal.
360. Sousa, R., Pilotto, F., Antunes, C., Aldridge, DC, Guilhermino, L. 2009. Invasive bivalves in
freshwater European ecosystems: ecological significance. 2nd European Congress of
Conservation Biology, Prague, Czech Republic. (Oral communication)
361. Stegner, A, Caldeira, R, Dong, C, Lazar, A. 2009. Three dimensional instabilities of an oceanic
vortex wake at large Reynolds number. 2nd International Conference on High-Reynolds Number
Vortex Interactions, 31 August-2 September, Brest, France. (Oral communication)
362. Stevens, MI, Magalhães, C, Ballted, RA, Storey, B, Cary, SC, Türk, R, Virginia, R, Wall, D. 2009. At
the limits of life: multidisciplinary insight from the Transantarctic Mountains. Xth SCAR
International Biology Symposium, 26-31 July, Sapporo, Japan.
363. Stoichev, T, Baptista, MS, Basto, MCP, Vasconcelos, V, Vasconcelos, MTSD. 2009. Fate and effects
of emerging organic contaminant in non-target species: a case study with the cyanobacterium
Microcystis aeruginosa. 14th International Symposium on Toxicity Assessment, 30 August-4
September, Metz, France. (Oral communication)
364. Stoichev, T, Baptista, MS, Basto, MCP, Vasconcelos, V, Vasconcelos, MTSD. 2009. Effects of the
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antibiotic minocycline in the cyanobacterium Microcystis aeruginosa growth. 1 Iberian Congress
in Cyanotoxins, 6-7 July, Porto, Portugal.
365. Tambourgi, MRS, Hazin, FHV, Oliveira, PGV, Coelho, R, Rêgo, M, Carvalho, F, Coutinho, IM,
Santos, NL, Roque, PCG. 2009. Project “Oceanic Sharks of Brasil”: a survey on shark catches by
pelagic longliners in the tropical Atlantic Ocean. 2nd Marine Biology Congress, 24-28 May, Búzios,
Brazil.
366. Teixeira, CF, Magalhães, C, Joye, S, Bordalo, AA. 2009. Effect of salinity on the fate of dissolved
inorganic nitrogen in estuarine sediments. BioMicroWorld - III International Conference on
Environmental, Industrial and Applied Microbiology, 2-4 December, Lisbon, Portugal.
367. Teixeira, CF, Magalhães, C, Joye, SB, Bordalo, AA. 2009. Patterns of anaerobic ammonium
oxidation activity in temperate estuarine sediments. American Society of Limnology and
Oceanography - ASLO Aquatic Science Meeting, 25-30 January, Nice, France.
368. Tiago, DM, Ferraresso, S, Romualdi, C, Bargelloni, L, Laizé, V, Cancela, ML. 2009. Global analysis
of gene expression in mineralizing fish bone derived cell lines. Interdisciplinary Approaches in
Fish Skeletal Biology, 27-29 April, Tavira, Portugal.
369. Tsigenopoulos, CS, Chatziplis, D, Louro, B, Vogiatzi, E, Sarropoulou, E, Bargelloni, L, Patarnello, T,
Power, DM, Canario, AVM, Magoulas, A, Kotoulas, G. 2009. Second generation genetic linkage
map for the gilthead sea bream Sparus aurata L. Genomics in Aquaculture International
symposium, 5-7 July, Bodo, Norway.
370. Turja, R, Packalén, A, Guimarães, L, Kankaanpää, H, Korpinen, S, Lehtonen, K. 2009. Combined
effects of exposure to extracts of the toxic cyanobacteria Nodularia spumigena and
benzo[a]pyrene on oxidative stress biomarkers in Gammarus oceanicus (Crustacea: Amphipoda).
SETAC Europe 19th Annual Meeting - Protecting Ecosystem Health: Facing the Challenge of a
Globally Changing Environment, 31 May-4 June, Gothenburg, Sweden.
371. Urbatzka, R, Castro, LFC, Rocha, MJ, Lobo-da-Cunha, A, Monteiro, RAF, Rocha, E. 2009. Purine
catabolism as new target of estrogen signalling? Cloning of urate oxidase in brown trout and
expression during the annual reproductive cycle. PRIMO 15 - 15th International Symposium on
Pollutant Responses in Marine Organisms, 17-20 May, Bordeaux, France.
372. Valentim, AL, Martins, GM, Neto, AI. 2009. Are seawalls made of natural substrata better?
International Meeting on Marine Resourses, 16-18 November, Peniche, Portugal.
373. Varela-Álvarez, E, Neto-Tiago, M, Glenn, T, Duarte, CM, Mártinez-Daranas, B, Valero, M, Marbá,
N, Fourquerean, JF, Serrão, E. 2009. Genetic variability and clonal structure in the genus
Caulerpa, an invasive green alga, revealed by microsatellite. BIOLIEF - World Conference on
Biological Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal.
374. Varela-Álvarez, E, Serrão, E. 2009. First record of the genus Convolutriloba, an invasive flatworm,
on the North East Atlantic assessed by DNA barcoding. BIOLIEF - World Conference on Biological
Invasions and Ecosystem Functioning, 27-30 October, Porto, Portugal.
375. Vasconcelos, MTSD, Stoichev, T, Basto, MCP, Vasconcelos, VM, Baptista, MS. 2009. Effects of the
antibiotic minocycline in the cyanobacterium Microcystis aeruginosa growth. 12th EuCheMS International conference on Chemistry and the Environment, 14-17 June, Stockholm, Sweden.
376. Veiga, P, Ribeiro, J, Gonçalves, J, Ditton, RB, Erzini, K. 2009. Assessment of recreational shore
fishing catch and effort in the south Portugal: a 12 month survey. 8th Indo Pacific Fish
Conference and 2009 ASFB Workshop & Conference, 31 May-5 June, Fremantle, WA, Australia.
377. Velez, Z, Hubbard, PC, Welham, KJ, Barata, EN, Hardege, JD, Canario, AV. 2009. Functional
asymmetry in the olfactory system of a flatfish, the Senegalese sole (Solea senegalensis). Annual
Meeting of the Society for Experimental-Biology, 28 June-1 July 2009, Glasgow, Scotland.
Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology 153A(2): S90S90. (Oral communication)
378. Vieira, L, Gravato, C, Soares, A, Morgado, F, Guilhermino, L. 2009. Acute effects of two metals on
Pomatoschistus microps: linking biochemical responses to behaviour. SETAC Europe 19th Annual
Environment, 31 May-4 June, Gothenburg, Sweden.
379. Vilar, R, Babichenko, S, Bastos, L, Palenzuela, JT, Martín-Herrero, J, Utkin, AB, Lavrov, A. 2009.
Detection and evaluation of oil spills by optical methods. 4th EARSEL Workshop on Remote
Sensing of Coastal Zone, 18-20 June, Chania, Crete, Greece. (Oral communication)
380. Voelker, AHL, Lebreiro, S. 2009. Deep water properties in the North Atlantic’s eastern and
western basins during the Mid-Brunhes (330-630 Ka). European Geosciences Union General
Assembly, 20-24 April, Vienna, Austria. Geophysical Research Abstracts, vol. 11, EGU2009-5782.
381. Voelker, AHL, Lebreiro, S, Lopes, C. 2009. See-saw patterns in intermediate and deep waters of
the North Atlantic Ocean linked to millennial-scale climate variability. Chapman Conference on
Abrupt Climate Change, 15-19 June, Byrd Polar Research Center, Ohio State University, Ohio,
USA. (Invited Oral communication)
382. Wallenstein, FM, Torrão, DF, Neto, AI, Wilkinson, M, Rodrigues, AS. 2009. Studies on Fucus
spiralis subject to increased temperature and acidity. 7th Asia-Pacific Conference on Algal
Biotechnology, 1-4 December, Delhi, India.
383. Wallenstein, FM, Torrão, DF, Neto, AI, Wilkinson, M, Rodrigues, AS. 2009. Accumulation of
metals in Fucus spiralis subject to increased temperature and acidity. 9th International
Phycological Congress, 2-8 August, Tokyo, Japan. Phycologia 48(4) Supplement: 92.
384. Wilson, JM, Bastos, E, Reis-Santos, PN, McCormick, SD. 2009. Identification of a branchial organic
anion transport protein (OATP) in the lamprey (Petromyzon marinus L.). Society for Experimental
Biology, 28 June-1 July, UK.
385. Wilson, JM, Goncalves, AF, Ings, J, Damasceno-Oliveira, A, Aluru, N, Vijayan, MM, Coimbra, J.
2009. Hepatic transcriptomics in response to hydrostatic pressure (3 MPa) in the shallow water
teleost Oncorhynchus mykiss. XIth International Meeting on High Pressure Biology, 31 August-1
September, Brest, France. (Oral communication)
386. Wilson, JM, Goncalves, AF, Ings, J, Damasceno-Oliveira, A, Aluru, N, Vijayan, MM, Coimbra, J.
2009. Hepatic transcriptomics in response to hydrostatic pressure (3 MPa) in the shallow water
teleost Oncorhynchus mykiss. Society for Experimental Biology, 28 June-1 July, UK.
COMMUNICATIONS IN NATIONAL SCIENTIFIC MEETINGS
1.
Almeida, R, Gomez-Zavaglia, A, Kaczor, A, Eusébio, MES, Maria, TMR, Cristiano, ML, Fausto, R.
2009. Mechanistic studies on the thermal isomerization of saccharyl ethers. 8º Congresso de
Química Física da Sociedade Portuguesa de Química, 15-16 Junho, Luso, Portugal. (Oral
communication)
2.
Antunes, A. 2009. Insights into the evolutionary genomics of mammalian species. 3 Jornadas de
Biologia na Universidade de Trás-os-Montes e Alto Douro, 21-22 Outubro, Vila Real, Portugal.
(Oral communication).
3.
Antunes, C, Rey, J. 2009. Contribuição para um modelo de gestão participada. O caso da bacia
hidrográfica do rio Minho e o projecto Natura Miño-Minho. I Seminário sobre Gestão de Bacias
Hidrográficas, 6-7 Maio, Faculdade Engenharia Universidade do Porto, Porto, Portugal. (Oral
communication)
as
4. Arenas, F. 2009. Biodiversity-Ecosystem functioning relationships…are natural systems following
our new theories? Evidence from intertidal marine assemblages. Seminário Oceanus, CIIMAR, 23
Março, Porto, Portugal. (Oral communication)
5. Bastos, L, Bio, A, Silva, AJ. 2009. Evolução da restinga do Douro 2001/2009 - dinâmica e possíveis
causas. Apresentado na sessão pública sobre o futuro Plano de Ordenamento do Estuário do
Douro, ARH, 23 Novembro, ICBAS, Porto, Portugal. (Oral communication)
6. Bensimon-Brito, A, Cancela, ML, Huysseune, A, Witten, PE. 2009. Ontogeny of zebrafish (Danio
rerio) caudal skeleton - a model for vertebral fusion. IGC Embryology Club, 2 Julho, Oeiras,
7. Bensimon-Brito, A, Huysseune, A, Witten, PE, Cancela, ML. 2009. Insights into molecular and
cellular events involved in human pathological fusion of vertebral joints through the use of
zebrafish as model organism. Seminários Mar e Ambiente, 8 Maio, Faro, Portugal.
8. Bos, MS, Arnoso, J, Benavent, M. 2009. Analysis of tidal gravity measurements made in the
Canary Islands. Encontro Ciência 2009, 29-30 Julho, Lisboa, Portugal. (Oral communication)
9. Bos, MS. 2009. Observing the orthometric height of the Kilimanjaro. Centro de Física do Porto, 20
Novembro, Porto, Portugal. (Oral communication)
10. Cabral, JP. 2009. Rocha Peixoto e a História Natural. Ensino, colecções e museus. Integrado no
Colóquio Rocha Peixoto no centenário da sua morte, Museu Municipal de Etnografia e História
da Póvoa de Varzim, 8-9 Maio, Póvoa de Varzim, Portugal. (Oral communication)
11. Cardoso, J. 2009. Age determination in bivalves: validation of the seasonality in growth bands.
Seminário Oceanus, CIIMAR, 17 Dezembro, Porto, Portugal. (Oral communication)
12. Campos, A. 2009. Characterization of Zea mays L. transglutaminase expression and interactions
with thylakoid membrane proteins. Seminário Oceanus, CIIMAR, 28 Setembro, Porto, Portugal.
13. Conceição, N, Cox, CJ, Simões, B, Viegas, M, Cancela, ML. 2009. Computational identification of
regulatory motifs in cartilage-expressed genes using zebrafish as an in vivo model. XVI Congresso
Nacional de Bioquímica, 22-25 Outubro, Açores, Portugal.
14. Carvalho, I, Cancela, ML, Rosenbaum, H. 2009. Population structure of humpback whale
(Megaptera novaeangliae) in west coast of Africa. Seminários Mar e Ambiente, 8 Maio, Faro,
15. Cristiano, MLS. 2009. New Approaches to Antiplasmodial Drugs. 8º Encontro de Química
Orgânica da Sociedade Portuguesa de Química, 1-3 Julho, Aveiro, Portugal. (Oral communication)
16. Costa, J, Santos, ES, Monteiro, OC, Costa, MC. 2009. Bio-synthesis of nanosized metal sulphide
semiconductors. MicroBiotec 2009, 29-30 Novembro, Vilamoura, Portugal.
17. Cox, CJ. 2009. The application of non-stationary composition models in phylogenetics. 5º
Encontro Nacional de Biologia Evolutiva, ISPA, 21 Dezembro, Lisboa, Portugal. (Oral
communication)
18. Deurloo, R, Bastos, L, Bos, M. 2009. Assessing the Use of UAV’s for Airborne Gravimetry. VI
Conferência Nacional de Cartografia e Geodesia - CNCG 2009, 7-8 Maio, Caldas da Rainha,
19. Dias, E, Carvalho, F, Amorim, A, Correia, AM, Santos, MJ, Antunes, JC. 2009. Macroparasites
survey in young European flounder (Platichthys flesus) on Minho estuary. IJUP 09 – II Encontro de
Jovens Investigadores da Universidade do Porto, 25-27 Fevereiro, Porto, Portugal.
20. Fernandes, MJ, Nunes, AL, Lázaro, C, Bastos, L, Mendes, VB, Pires, N. 2009. Correcção do atraso
troposférico nas medidas de altimetria por satélite em zonas costeiras usando técnicas GNSS. VI
Conferência Nacional de Cartografia e Geodesia - CNCG 2009, 7-8 Maio, Caldas da Rainha,
21. Ferreira, M, Reva, I, Araújo, NCP, Guerreiro, BEC, Goméz-Zavaglia, A, Cristiano, MLS, Fausto, R.
2009. Structure and reactivity of endoperoxides with antimalarial activity. 8º Encontro de
Química Orgânica da Sociedade Portuguesa de Química, 1-3 Julho, Aveiro, Portugal.
22. Fidalgo, ML. 2009. Ambiente e desenvolvimento: tensão ou complementaridade? Colóquio
Utopia, Urbanismo e Ambiente, 20-21 Março, Valongo, Portugal. (Oral communication)
23. Fidalgo, ML. 2009. Ambiente e desenvolvimento: Tensão ou complementaridade? Colóquio
Utopia, Urbanismo e Ambiente, 20-21 Março, Valongo, Portugal. (Oral communication)
24. Fonseca, CSC, Ciobanu, I, Cristiano, MLS. 2009. Heterogeneous catalytic transfer reduction of
aldimines. 8º Encontro de Química Orgânica da Sociedade Portuguesa de Química, 1-3 Julho,
Aveiro, Portugal.
25. Gesto Rodriguez, M. 2009. Brain neurotransmission in fish as a target for the toxic effects of
polycyclic aromatic hydrocarbons (PAHs). Seminário Oceanus, CIIMAR, 17 Junho, Porto, Portugal.
26. Gonçalves, JA, Bastos, L, Granja, H, Magalhães, A, Madeira, S. 2009). Criação de modelos digitais
do terreno de zonas costeiras a partir de fotografia aérea digital. VI Conferência Nacional de
Cartografia e Geodesia - CNCG 2009, 7-8 Maio, Caldas da Rainha, Portugal.
27. Gonçalves, JMS, Monteiro, P, Veiga, P, Afonso, C, Rangel, M, Oliveira, F, Erzini, K, Bentes, L. 2009.
Cartografia das comunidades marinhas subtidais: Algarve central. Seminários do Mar, 7-8 de
Maio, Universidade do Algarve, Faro, Portugal.
28. Gonçalves, JMS, Erzini, K, Monteiro, P, Veiga, P, Afonso, C, Rangel, M, Oliveira, F, Bentes, L. 2009.
Planeamento espacial do meio marinho: conservação da biodiversidade. Workshop sobre o Plano
de Ordenamento Espacial do Meio Marinho POEM, Biblioteca Municipal de Olhão, 4 de Maio,
Olhão, Portugal. (Oral communication)
29. Guilhermino, L. 2009. Os recursos ecológicos das águas estuarinas e costeiras do Norte: as
ameaças e as oportunidades. Encontro do Litoral Norte, POLIS Litoral Norte – Sociedade para a
Requalificação e valorização do Litoral Norte e ARH Norte, 20 Novembro, Viana do Castelo,
30. Ismael, A, Silva, JP, Cristiano, MLS. 2009. Studies on the photochemistry of allylic derivatives of
tetrazole in cellulose. 8º Encontro de Química Orgânica da Sociedade Portuguesa de Química, 1-3
Julho, Aveiro, Portugal.
31. Magalhães. C. 2009. Dynamics of the marine nitrogen cycle: key processes, communities and
anthropogenic impacts. Seminário Oceanus, CIIMAR, 12 Novembro, Porto, Portugal. (Oral
communication)
32. Marques, CL, Laizé, V, Cancela, ML. 2009. New insights on osteogenic properties of BMP2 using a
fish model. Seminários do Mar e Ambiente, 8 Maio, Faro, Portugal.
33. Martins da Costa, P. 2009. Antibioterapia e resistência aos antibióticos. Ciclo de Conferências
“Uma só saúde”, Ordem dos Médicos Veterinários Portugueses, 16 Maio, Porto, Portugal.
(Comunicação oral)
34. Martins da Costa, P. 2009. Emergência de antibiorresistências na cadeia alimentar - perspectiva
do consumidor. III Ciclo de Conferências de Saúde Pública Veterinária, ICBAS, Universidade do
Porto, 3-4 Julho, Porto, Portugal. (Oral communication)
35. Mucha, AP. 2009. OILDEBEACH – Buried oil in the intertidal beach zone: coupling between beach
morphodynamic, natural degradation, forcing mechanisms and biological activity. Seminário
Oceanus, CIIMAR, 1 Junho, Porto, Portugal. (Oral communication)
36. Neto, AI. 2009. Biodiversidade litoral nos Açores: caracterização, ameaças, conservação. 8.º
Seminário Regional Eco-Escolas/Jovens Repórteres para o Ambiente, 21 Março, Ecoteca da
Lagoa, São Miguel, Açores, Portugal. (Oral communication)
37. Pereira, J, Baptista, MS, Stoichev, T, Heggie, B, Vasconcelos, VM, Vasconcelos, MTSD. Survey of
some pharmaceutics effects on freshwater cyanobacteria. IJUP 09 – II Encontro de Jovens
Investigadores da Universidade do Porto, 25-27 Fevereiro, Porto, Portugal.
38. Pereira Silva, M, Sousa Pinto, I. 2009. Biodiversity and Companies - European overview. 2º
Encontro Anual Business and Biodiversity - Concretização e Expectativas, 28 Maio, Lisboa,
39. Rangel, MO, Dentinho, T, Araújo, G, Lopes, J, Gonçalves, MS, Erzini, K. 2009. Análise custo de
viagem de roteiros subaquáticos (em apneia) na Praia da Marinha (Algarve). Turismo e
Sustentabilidade, 3º Workshop da APDR, Universidade dos Açores, 26-27 Abril, Ponta Delgada,
Açores, Portugal.
40. Roberto, V, Gautvik, KD, Cancela, ML. 2009. Zebrafish as a transgenic in vivo model to analyse
bone-related gene function and regulation of expression. Seminários do Mar e Ambiente, 8 Maio,
Faro, Portugal.
41. Roberto, VP, Tiago, DM, Laizé, V, Cancela, ML. 2009. Zebrafish as an in vivo and in vitro model to
analyze mir-223 target genes. RNA2009, 5-6 Novembro, Carcavelos, Portugal.
42. Rosa, JT, Cancela, ML, Laizé, V. 2009. Role of a short-chain dehydrogenase/ reductase in tissue
mineralization, Seminários Mar e Ambiente, 18 Dezembro, Faro, Portugal.
43. Sansana, FS, Pinto, JP, Caldeira, R, Tomé, R. 2009. Avaliação do campo de ventos de Mesoescala
sobre a previsão da agitação marí ma em torno do Arquipélago da Madeira. 6 Jornadas
Portuguesas de Engenharia Costeira e Portuária, 8-9 Outubro, Funchal, Madeira, Portugal. (Oral
communication)
44. Santos, P. 2009. A gestão de recursos vivos nos planos de ordenamento. Jornadas de Ambiente
do Sudoeste, Odemira, Portugal. (Oral communication)
45. Simes, DC, Viegas, CSB, Cancela, ML. 2009. Aplicações da análise proteómica 2D na identificação
de possíveis biomarcadores para o diagnostico/ prognostico de doenças, Seminários do Hospital
de Faro, 29 Outubro, Faro, Portugal. (Oral communication)
46. Simões, B, Conceição, N, Cancela, L, Kelsh, R. 2009. Runx3 structure and function in zebrafish
neural crest: expression pattern and morpholino knockdown. Seminários Mar e Ambiente, 8
Maio, Faro, Portugal. (Oral communication)
47. Simões, R, Poirel, L, Martins da Costa, P, Nordmann, P. 2009. Gaivotas como reservatório e vector
de Escherichia coli produtoras de ß-lactamases de espectro alargado. VI Congresso da Ordem dos
Médicos Veterinários, Lisboa, Portugal.
48. Sousa Pinto, I, Vieira, R. 2009. Os Ecossistemas Costeiros. Workshop NOAA – Explore, Centro
Ciência Viva, 7-8 Fevereiro, Vila do Conde, Portugal. (Oral communication)
49. Sousa, JJ, Ruiz, AM, Hanssen, RF, Bastos, L, Gil, AJ, Galindo-Zaldívar, J, Sanz de Galdeano, C. 2009.
Estudo comparativo: processamento PS-InSAR para detecção de deformações da crusta terrestre
– Caso de estudo: Bacia de Granada (Cordilheira Bética, Sudeste de Espanha). VI Conferência
Nacional de Cartografia e Geodesia - CNCG 2009, 7-8 Maio, Caldas da Rainha, Portugal. (Oral
communication)
50. Sousa, R. 2009. Aliens not coming from space. Universidade do Minho, Braga, Portugal. (Oral
communication)
51. Sousa, R. 2009. Are aquatic invasive species important? Seminário Oceanus, CIIMAR, 20 Abril,
Porto, Portugal. (Oral communication)
52. Tiago, DM, Laizé, V, Bargelloni, L, Ferraresso, S, Romualdi, C, Cancela, ML. 2009. Global analysis of
gene expression during in vitro mineralization reveals genes involved in anti-mineralogenic effect
of vanadate. RNA2009, 5-6 Novembro, Carcavelos, Portugal.
53. Tuya, F. 2009. Proximity to reefs alters the balance between positive and negative effects on
seagrass fauna. Seminário Oceanus, CIIMAR, 19 Outubro, Porto, Portugal. (Oral communication)
54. Wallenstein, FM, Torrão, DF, Neto, AI, Rodrigues, AS. 2009. Metal load in macroalgae from São
Miguel, a preliminary approach. Workshop Ecotoxicology and Risk Assessment, Universidade dos
Açores, Ponta Delgada, Portugal. (Oral communication)
55. Wurtz, S. 2009. Sturgeon restoration programme in Central Europe. Seminário Oceanus, CIIMAR,
11 Maio, Porto, Portugal. (Oral communication)
D.
PATENTS
1.
Cancela, ML, Laize, V, Leite, R, Danielsen, S. 2009. Patent 11577.000-EP.
2.
3.
4.
Costa, MC, Martins, M, Jesus, C, Barros, R. 2009. Uso de lama de mármore para pré-tratamento
químico de efluentes ácidos. Portuguese Patent DMP/01/2009/204286.
5.
Cruzeiro, L. 2009. Método para deduzir a estrutura tridimensional de uma proteína a partir da
sequência de aminoácidos. Portuguese Provisional Patent Application No. 104832, under
approval by the Portuguese Patent Office.
6.
Feed additives and their use for aquaculture and aquiculture. Portuguese Provisional Patent
Application No. 104732, under approval by the Portuguese Patent Office.
Information compiled by:
Susana Moreira
CIIMAR Science Office
Rua dos Bragas 289
4050-123 Porto
Portugal
Phone: +351 22 339 06 07
E-mail: [email protected]
Please use the above contact for data improvement.

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