1 EUPLAN Platelet Conference

1st EUPLAN Platelet Conference
th
September 19-21th 2012,
Grand Hotel de l’Empereur Maastricht, The Netherlands
WELCOME MESSAGE
A warm welcome to Maastricht, one of the oldest cities in the Netherlands with a southern
atmosphere, for this first meeting of the European Platelet Network (EUPLAN) in Grand Hôtel
de l’Empereur. The program, including a special clinical session, is very international and
exciting with 36 oral presentations and 98 poster presentations.
Europe has a strong history in platelet research with meetings at a regular base since 1983,
especially in Germany, the United Kingdom, Italy and France. In addition, the recently
revitalized European Thrombosis Research Organization (ETRO) and the various national
Thrombosis and Haemostasis Societies are active in stimulating and facilitating platelet
research. The EUPLAN initiative started in October 2009 in Toulouse, when leading platelet
scientists agreed that there is a need for regular meetings on a European level to exchange
ideas and to support high quality basic and clinical research on platelets and
megakaryocytes, next to the existing national meetings. However, the EUPLAN initiative
goes further than only organizing conferences (see website http://euplan.eu/).
The undertaking of the conference has been made possible by the generous support of our
sponsors (see previous and next pages). We are very grateful for this. Please, visit their
displays during the meeting.
We hope that you have a pleasant stay in Maastricht.
Johan Heemskerk and Judith Cosemans (on behalf of the organizing committee)
Local organizing committee
Contact EUPLAN Platelet conference
Dr. Judith Cosemans
Prof. dr. Hugo ten Cate
Prof. dr. Johan Heemskerk
Dr. Roosje van Gorp
Department of Biochemistry, CARIM
Maastricht University
P.O. Box 616
6200 MD Maastricht
The Netherlands
Tel. +31-43-3881671 (3881674)
Fax. +31-43-3884159
Department of Biochemistry, CARIM
Maastricht University
P.O. Box 616
6200 MD Maastricht
The Netherlands
Tel. +31-43-3881674 (3881671)
Fax. +31-43-3884159
[email protected] or
[email protected]
http://euplan.eu/
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GOLD SPONSORS
SILVER SPONSORS
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SILVER SPONSORS
BRONZE SPONSORS
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KEYNOTE & GUEST
SPEAKERS
INTERNATIONAL SCIENTIFIC
ADVISORY COMMITTEE
Prof. dr. Marco Cattaneo
Medicina III, Ospedale San Paolo
Università di Milano, Italy
Prof. dr. Hans Deckmyn
Laboratory for Thrombosis Research
KU Leuven, Campus Kortrijk, Belgium
Prof. dr. Scott Diamond
Institute for Medicine and Engineering
University of Pennsylvania
Philadelphia, USA
Prof. dr. Christian Gachet
INSERM UdS, EFS Alsace
Strasbourg, France
Dr. Laura Gutièrrez
Sanquin-CLB
Amsterdam, The Netherlands
Dr. Kathleen Freson
Center for Molecular and Vascular Biology
University of Leuven, Belgium
Prof. dr. Tomas Lindahl
Clinical Chemistry
Linköping University Hospital
Linköping, Sweden
Dr. Christian Hackeng
Department of Clinical Pharmacy
St. Antonius Hospital
Nieuwegein, The Netherlands
Prof. dr. Bernhard Nieswandt
Rudolf Virchow Centre
Julius Maximilians University Würzburg
Germany
Prof. dr. Paul Harrison
Haemophilia and Thrombosis Centre
Churchill Hospital
Oxford, United Kingdom
Prof. dr. Peter Presek
Clinical Pharmacology
Martin Luther University Halle-Wittenberg
Halle, Germany
Prof. dr. Berend Isermann
Internal Medicine & Clinical Chemistry
University of Heidelberg
Heidelberg, Germany
Prof. dr. Mauro Torti
Department of Biochemistry
University of Pavia,
Pavia, Italy
Dr. Mark Roest
Clinical Chemistry & Hematology
Utrecht Medical Center, The Netherlands
Prof. dr. William Vainchenker
Institute Gustave Roussy
UMR1009, Villejuif
France
Dr. Albert Smolenski
Conway Institute
University College Dublin, Ireland
Prof. dr. Thomas Unger
Cardiovascular Research Institute
Maastricht (CARIM)
Maastricht, The Netherlands
Dr. Ivo Volf
Institute of Physiology
Center for Physiology & Pharmacology
Medical University of Vienna
Vienna, Austria
Prof. dr. William Vainchenker
Institute Gustave Roussy
UMR1009, Villejuif, France
Prof. dr. Steve Watson
Cardiovascular & Cellular Pharmacology
Medical School, University of Birmingham
United Kingdom
Prof. dr. Steve Watson
Cardiovascular & Cellular Pharmacology
Medical School, University of Birmingham
United Kingdom
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SCIENTIFIC PROGRAMME
Wednesday, 19th September 2012
13.30 - 17.30
Special methods workshops (only for participants registered for the
workshops). Location: Department of Biochemistry, Maastricht
University, Universiteitssingel 50, Maastricht

Multi-parametric analysis of thrombus formation at physiological
shear flow – in collaboration with Westburg/Fluxion Biosciences
(Timo Kreike)

Application of advanced microscopy in studies of in vivo and in vitro
thrombus formation – Biochemistry Department, CARIM, Maastricht
University (Judith Cosemans, Johan Heemskerk)

Platelet function analyses on the BD Accuri C6 cytometer – in
collaboration with BD (Paul Harrison)

Desktop scanning electron microscopy – in collaboration with
Phenom-World (Bas de Laat, Fleur van Wely)
18.00 -
Registration with bits and bites
Location: Amrâth Grand Hôtel de l’Empereur, Maastricht
19.00 - 21.00
Special clinical session: Platelets, bleeding and pro-hemostatics
(open to all participants). Location: Amrâth Grand Hotel de
l’Empereur, Maastricht
Chairs: Johan Heemskerk and Judith Cosemans
19.00
Stroke still needs to be prevented: does anticoagulation beat antihypertension?
Thomas Unger (Maastricht, The Netherlands)
19.25
Two-step tests to measure platelet function and von Willebrand
factor in whole blood
Mark Roest (Utrecht, The Netherlands)
19.50
How to implement flow cytometry for platelet function analysis in the
clinic?
Paul Harrison (Oxford, United Kingdom)
20.05
Omics approaches to expand our genotype-phenotype knowledge of
inherited platelet disorders
Kathleen Freson (Leuven, Belgium)
20.30
When does a defect in platelet function give rise to bleeding?
Steve Watson (Birmingham, United Kingdom)
21.00
Welcome drink
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Thursday, 20th September 2012
08.50 - 09.00
Welcome: Johan Heemskerk, Judith Cosemans
09.00 - 10.45
Session I: Clinical aspects
Chairs: Christian Gachet and Peter Presek
09.00
Key note lecture: Antiplatelet drugs in patients
(non)invasive cardiac interventions
Christian Hackeng (Nieuwegein, The Netherlands)
09.30
O1. Lentiviral gene rescue in a Bernard-Soulier mouse model
provides a tool to assess the function of GPIb in vivo
undergoing
Catherine Strassel, Anne Bull, Sylvie Moog, Stephanie Magnenat, Marie Jeanne
Baas, Monique Freund, Christian Gachet, François Lanza (Strasbourg, France)
09.45
O2. Only severe thrombocytopenia
insufficient thrombus formation in mice
results
in
bleeding
and
Martina Morowski, Timo Vögtle, Peter Kraft, Guido Stoll, Bernhard Nieswandt
(Würzburg, Germany)
10.00
O3. Platelet phenotype and thrombosis in JAK2V617F mice
Lamia Lamrani, Catherine Lacout, Véronique Ollivier, Benoit Ho Tin Hoe, William
Vainchenker, Jean Luc Villeval, Martine Jandrot-Perrus (Paris, Villejuif, France)
10.15
O4. Platelet Hyperreactivity Project: a network-based approach to
elucidate determinants of platelet reactivity in aspirin-treated
cardiovascular patients
A. Zufferey, M. Ibberson, J.-L. Reny, S. Nolli, M. Docquier, I. Xenarios, J.C.
Sanchez, P. Fontana (Geneva, Lausanne, Switzerland)
10.30
O5. Prospective evaluation of platelet activation markers in patients
treated with drug-eluting stents: platelet-associated tissue factor
predicts the risk of future revascularization
Marina Camera, Marta Brambilla, Daniela Trabattoni, Fabrizio Veglia, Paola
Canzano, Laura Rossetti, Antonio Bartorelli, Elena Tremoli (Milano, Italy)
10.45 - 11.15
Coffee break
11.15 - 12.45
Session II: Platelet development
Chairs – Marc Hoylaerts and Bernard Payrastre
11.15
Key note lecture: RUNX1 and megakaryopoiesis
William Vainchenker (Villejuif, France)
11.45
O6. Megakaryocyte-specific deletion of the protein-tyrosine
phosphatases Shp1 and Shp2 causes a block in megakaryocyte
development and severe macrothrombocytopenia
Alexandra Mazharian, Jun Mori, Ying-Jie Wang, Silke Heising, Benjamin G. Neel,
Steve P. Watson and Yotis A. Senis (Birmingham, Oxford, United Kingdom;
Toronto, Canada)
12.00
O7. BAMBI (BMP and activin membrane bound inhibitor): a novel
regulator of platelet production and function
Isabelle I. Salles, David A. Lane, James T.B. Crawley (London, United Kingdom)
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12.15
O8. Role of p110beta and alpha isoforms of class I PI3-kinases in
platelet activation and functions
Sonia Séverin, Pierre-Alexandre Laurent, Gaëtan Chicanne, Béatrice Hechler,
Christian Gachet, Julie Guillermet-Guibert, Bart Vanhaesebroeck, Bernard
Payrastre, Marie-Pierre Gratacap (Toulouse, France)
12.30
O9. Maps of open chromatin highlight cell type-specific patterns of
regulatory sequence variation at haematological trait loci
Augusto Rendon, Dirk S. Paul, Cornelis A. Albers, Katrin Voss, Jonathan
Stephens, HaemGen Consortium, Pim van der Harst, John C. Chambers, Nicole
Soranzo, Willem H. Ouwehand, Panos Deloukas (Cambridge, Hinxton, London,
Middlesex, United Kingdom; Groningen, The Netherlands)
12.45 - 14.15
Lunch
14.15 - 15.45
Session III: Thrombosis and thrombus formation
Chairs – Elisabeth Gardiner and Bernhard Nieswandt
14.15
Key note lecture: P2Y12 inhibitors: not only P2Y12, not only platelets,
not only thrombosis
Marco Cattaneo (Milano, Italy)
14.45
O10. Gap junctions and
haemostasis and thrombosis
connexin
hemichannels
underpin
Sakthivel Vaiyapuri, Chris I. Jones, Parvathy Sasikumar, Leonardo A. Moraes,
Marfoua S. Ali, Tanya Sage, Alexander M. Simon, Martyn P. Mahaut-Smith,
Jonathan M. Gibbins (Reading, Leicester, United Kingdom; Tucson, USA)
15.00
O11. Major role for integrin 61 in platelet adhesion and
aggregation to an injured arterial wall
Mathieu Schaff, ChaoJun Tang, Eric Maurer, Catherine Bourdon, Nicolas
Receveur, Béatrice Hechler, Adèle De Arcangelis, Elisabeth Georges-Labouesse,
Christian Gachet, François Lanza, Pierre H. Mangin (Illkirch-Graffenstaden,
Strasbourg, France)
15.15
O12. P2Y12 synergistically controls a Munc13-4-independent pathway
to secretion of α-granules but not dense granules in platelets
Matthew T. Harper, Joshua S. Savage, Christopher M. Williams, Alastair W. Poole
(Bristol, United Kingdom)
15.30
O13. Grb2 is essential for GPVI-mediated platelet activation
S. Dütting, T. Vögtle, M. Morowski, P. Kraft, G. Stoll, L. Nitschke, B. Nieswandt
(Würzburg, Erlangen, Germany)
15.45 - 17.15
Poster presentation session A
18.00 – 24.15
Social and culinary program:
Maastricht event! Walking tour with dinner starts from Amrâth Grand Hôtel
de l’Empereur. A spectacular location is visited in the end. Back at walking
distance from the hotel around 24 h. Earlier return is possible refreshments included
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Friday, 21th September 2012
08.45 - 10.40
Session IV: Platelets in coagulation, inflammation & reproduction
Chairs – Hans Deckmyn and Tomas Lindahl
08.45
Key note lecture: Defining the role of platelets during reproduction
Berend Isermann (Heidelberg, Germany)
09.15
O14. Targeting factor XII inhibits the pathological process of
thrombus formation on ruptured plaques in vivo and in vitro
Marijke J.E. Kuijpers, Marion A.H. Feijge, Paola E.J. van der Meijden, Thomas
Renné, Johan W.M. Heemskerk, Judith M.E.M. Cosemans (Maastricht, The
Netherlands; Stockholm, Sweden)
09.30
O15. Platelets directly enhance transmigration of neutrophils in
response to oxidized LDL
Sigrun Badrnya, Lynn M. Butler, Cecilia Söderberg-Naucler, Ivo Volf, Alice
Assinger (Stockholm, Sweden; Vienna, Austria)
09.45
O16. Coagulation factor XII is not activated by platelets but
facilitates platelet-driven propagation of coagulation
Lars Faxälv, Sofia Ramström, Kristina Soutukorva, Pentti Tengval, Tomas L.
Lindahl (Linköping, Gothenburg, Sweden)
10.10
O17. Neutrophils initiate coagulation on the injured vessel wall
Roxane Darbousset, Grace M. Thomas, Soraya Mezouar, Nigel Mackman,
Thomas Renné, Françoise Dignat-George, Christophe Dubois, Laurence
Panicot-Dubois (Marseille, France; Boston, Chapel Hill, USA; Stockholm,
Sweden)
10.25 - 12.00
Poster presentation session B (coffee)
12.00 - 13.20
Lunch
13.20 - 15.00
Session V: Platelet signaling
Chairs – Ingeborg Hers and Khalid Naseem
13.20
Key note lecture: Cyclic nucleotides, kinases and signaling in
platelets
Albert Smolenski (Dublin, Ireland)
13.50
O18. A point mutation in CalDAG-GEFI gene affects inside-out
signaling and platelet spreading in humans
Matthias Canault, Charlotte Grosdidier, Pierre E. Morange, Marie Guinier,
Véronique Baccini, Alan T. Nurden, David Tregouet, Marie-Christine Alessi
(Marseille, Paris, France)
14.05
O19. PKCα and Akt -mediated phosphorylation of GSK3 promotes
integrin activation and granule secretion in platelets
Samantha F. Moore, Marion van den Bosch, Ingeborg Hers (Bristol, United
Kingdom)
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14.20
O20. Oxidized low-density lipoproteins induce platelet activation and
shape change through a novel CD36-Rho kinase dependent
signalling pathway
Katie S. Wraith, Simbarashe Magwenzi, Ahmed Aburima, Yichuan Wen, David
Leake, Khalid M. Naseem (Hull, Reading, United Kingdom)
14.35
O21. Dual mechanism of integrin closure in procoagulant platelets
Nadine J.A. Mattheij, Karen Gilio, Roger van Kruchten, Shawn M. Jobe, Adam J.
Wieschhaus, Athar H. Chishti, Peter W. Collins, Johan W.M. Heemskerk, Judith
M.E.M. Cosemans (Maastricht, The Netherlands; Atlanta, Boston, USA; Cardiff,
United Kingdom)
14.50 - 15.20
Coffee break
15.20 - 16.50
Session VI: New approaches and directions in platelet research
Chairs Mauro Torti and Ivo Volf
15.20
Key note lecture (supported by Netherlands Heart Foundation): Systems
biology approaches in platelet research
Scott Diamond (Philadelphia, PA, USA)
15.50
O22. G6f-like is an ITAM containing collagen receptor mediating fish
haemostasis
Craig E. Hughes, Uvaraj P. Radhakrishnan, Stuart Egginton, Johannes M.
Dijkstra, Jagadeeswaran Purdur, Steve P. Watson (Birmingham, United Kingdom;
Denton, Texas, USA; Toyoake, Aichi, Japan)
16.05
O23. Systematic characterization of human platelets by quantitative
proteomics
Julia Burkhart, Marc Vaudel, Florian Beck, Stepan Gambaryan, Ulrich Walter,
Albert Sickmann, Jörg Geiger, René Zahedi (Dortmund, Würzburg, Mainz,
Germany)
16.20
O24. Thrombin stimulation induces de novo SDF-1a synthesis and
alters microRNA profile in human platelets
Zhangsen Huang, Mohammed Ferdous-Ur Rahman, Lei Jiang, Hong Xie, Stefano
Caramuta, Weng-Onn Lui, Nailin Li (Stockholm, Sweden)
16.35
O25. RGS18 integrates activatory and inhibitory signalling in
platelets
Kristina Gegenbauer, Giuliano Elia, Alfonso Blanco-Fernandez, Albert Smolenski
(Dublin, Ireland)
16.50 – 17.00
Farewell and departure
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POSTER PRESENTATION SESSION A. Thursday 20th September 2012
All abstract scored by the international scientific advisory committee
P1
Time- and cost-effective method for monitoring platelet function using a new
standardized automated high-throughput test system based on light
transmission aggregometry
F. Prüller, S. Klinger, F. Fruhwald, S. Macher, A.C. Haushofer, R.B. Raggam, M.
Truschnig, E. Mahla (St. Pölten, Graz, Austria)
P2
The platelet tetraspanin Tspan9 is required to fine tune GPVI signalling
Elizabeth J. Haining, Jing Yang, Marie Lordkipanidzé, Brenda A. Finney, Craig E.
Hughes, Steve P. Watson, Michael G. Tomlinson (Birmingham, United Kingdom)
P3
P2Y12 signalling is critical for sustained activation of Rap1b in human platelets
Hariharan Subramanian, Ulrich Walter, Stepan Gambaryan (Würzburg, Germany)
P4
Six-biomarker algorithms identify Alzheimer's disease at high accuracy
Maria Zellner, Alexandra Graf, Michael Veitinger, Rita Babeluk, John Lamont, Ellen
Umlauf (Vienna, Austria; Crumlin, United Kingdom)
P5
Platelet effects on neutrophils: the yin and the yang
Benoît Ho-Tin-Noé, Lamia Lamrani, Véronique Ollivier, Stéphane Loyau, Martine
Jandrot-Perrus (Paris, France)
P6
Alterations of platelet hemostatic markers during storage of platelet
concentrates
Alfonso Vignoli, Marina Marchetti, Carmen Julia Tartari, Laura Russo, Erika Diani,
Cinzia Giaccherini, Cristina Verzeroli, Luca Da Prada, Stefana Marziali, Anna Falanga
(Bergamo, Italy)
P7
The hydrogen sulfide releasing aspirin derivative ACS14 exerts strong
cyclooxygenase independent antiplatelet effects
Joachim Pircher, Franziska Fochler, Thomas Czermak, Hanna Mannell, Piero Del
Soldato, Ulrich Pohl, Florian Krötz (Munich, Germany; Milano, Italy)
P8
Regulation of platelet GPCR receptor function by NHERF protein isoforms in
mouse platelets
Robert J.P. Pope, Shaista P. Nisar, Margaret R. Cunningham, Alastair W. Poole, Stuart
J. Mundell (Bristol, United Kingdom)
P9
Human circulating microparticles: a global assessment of their physical and
antigenic proprieties by a multicolor flow cytometry approach
Daniela Boselli, Marta Brambilla, Daniele Manganaro, Elena Tremoli, Marina Camera.
(Milano, Italy)
P10
Redundant functions of phospholipases D1 and D2 in platelet -granule release
Ina Thielmann, David Stegner, Peter Kraft, Ina Hagedorn, Christoph Kleinschnitz,
Guido Stoll, Bernhard Nieswandt (Würzburg, Germany)
P11
Inverse role of pulsatile blood flow on thrombus growth under physiological and
disturbed rheological conditions
Eric Maurer, Christian Gachet, François Lanza, Pierre Mangin (Strasbourg, France)
10
P12
Analysis of hemostatic properties of blood platelets after treatment with different
forms of homocysteine in vitro
Joanna Malinowska and Beata Olas (Lodz, Poland)
P13
Synaptotagmin-like protein 4 regulates platelet granule secretion
Ashling Hampson, Albert Smolenski (Dublin, Ireland)
P14
High on-treatment platelet reactivity (HPR) during clopidogrel treatment in minor
stroke or TIA: impact of glucose metabolism and insulin resistance
Annika Lundström, Ann-Charlotte Laska, Magnus von Arbin, Håkan Wallén
(Stockholm, Sweden)
P15
Multiparameter assessment of thrombus formation: a systems biology approach
Susanne M. de Witt, Moniek M.E. Lamers, Frauke Swieringa, Roger van Kruchten,
Richard W. Farndale, Kenneth J. Clemetson, Judith M.E.M. Cosemans, Johan W.M.
Heemskerk (Maastricht, The Netherlands; Cambridge, United Kingdom; Bern,
Switzerland)
P16
In vivo efficacy of platelets produced by megakaryocyte exposure to high shear
rates
Sonia Poirault-Chassac, Audrey Pietrzyk, Evelyne Lauret, Cécile V. Denis,
Dominique Baruch (Paris, Le Kremlin-Bicêtre, France)
P17
Romiplostim administration shows reduced megakaryocyte response capacity
and increased myelofibrosis in a mouse model of MYH9-RD
Catherine Léon, Katja Evert, Frank Dombrowski, Fabien Pertuy, Anita Eckly, Patricia
Laeuffer, Christian Gachet, Andreas Greinacher (Strasbourg, France)
P18
Evaluation of a new flow cytometric bead assay for VASP-phosphorylation to
assess the effectiveness of P2Y12 antagonists in patients with acute coronary
syndrome and its comparison with the Biocytex VASP test
Natalia Dovlatova, Jacqueline Glenn, Ann White, Stan Heptinstall, Susan Fox
(Nottingham, United Kingdom)
P19
Dimers of the platelet collagen receptor GPVI are essential for platelet adhesion
and activation
Stephanie M. Jung, Masaaki Moroi, Nicholas Pugh, Steve P. Watson, Richard W.
Farndale (Birmingham, Cambridge, United Kingdom)
P20
Misfolded extracellular proteins activate plateletsby interaction with the stress
response cell surface chaperones, glucose-regulated protein (GRP) 78/BIP, and
thiol isomerases
Anne Bertling, Kerstin Jurk, Martin F. Brodde, Christopher Stavermann, Meike Horn,
Muzaffar Hussain, Christina Stürzel, Anja Müller, Jerzy-Roch Nofer, Judith Lahav, Hugo
Van Aken, Christine Heilmann, Beate E. Kehrel (Münster, Germany; Petah Tikva,
Israel)
P21
Functional and morphological characterization of the three activated platelet
subpopulations
Sergey I. Obydennyi, Nadezhda A. Podoplelova, Faina Y. Verholomova, Elena O.
Artemenko, Natalia V. Zakharova, Mikhail A. Panteleev (Moscow, Russia)
P22
Characterization of novel tyrosine kinase pathways regulating platelet
functional responses
Todd Getz, Lorena Butriago, Yingying Mao, Satya P. Kunapuli (Philadelphia, USA)
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P23
In human platelets the multidrug resistance protein 4 (MRP4) is induced by
aspirin
Isabella Massimi, Raffaella Guerriero, Lavinia Vittoria Lotti, Valentina Lulli, Federico
Romani, Luigi Frati, Fabio M. Pulcinelli (Rome, Italy)
P24
N-octanoyl-dopamine is a potent inhibitor of platelet function
Angelika Schedel, Lamia Ait-Hsiko, Tineke Kraaij, Bastian Theisinger, Benito Yard,
Peter Bugert (Baden-Württemberg, Mannheim, Heidelberg, Germany)
P25
Comprehensive analysis of the CLEC-2 signaling cascade by platelet
proteomics: novel clues on CLEC-2 signaling regulation
Andrés F. Parguiña, Isaac Rosa, Johannes A. Eble, Ángel García (Santiago de
Compostela, Spain; Frankfurt am Main, Germany)
P26
Development and characterization of a dimeric peptidomimetic of platelet GPVI
Peng Jiang, Jérome Hérault, Faisal Alshoukr, Rana Benazzouna, Laure Sarda-Mantel,
Chang-Zhi Dong, Martine Jandrot-Perrus (Paris, France)
P27
Low biological variation protein characterization by 2D DIGE as new putative
normalization standards
R. Baumgartner, M. Veitinger, E. Umlauf, S. Guterres, I.Volf, J. Lamont, R. Öhler, M.
Zellner (Crumlin, United Kingdom; Vienna, Austria)
P28
Oxidised LDL activates platelets NADPH oxidase-dependent inhibition of the
cGMP/protein kinase G signalling cascade
Simbarashe Magwenzi, Catriona McNeil, Katie Wraith, Khalid Naseem (Hull, United
Kingdom)
P29
Effects of grape seed extract on antiplatelet function of endothelial cells
Michał Bijak, Bogusława Luzak, Anna Kosiorek, Kamila Syska, Joanna Saluk, Marek
Rożalski, Cezary Watala, Paweł Nowak, Barbara Wachowicz, Jacek Golański (Lodz,
Poland)
P30
Automated analysis of dynamic in vitro thrombus formation
Loic Peter, Sjoert B.G. Jansen, Willem H. Ouwehand, Nassir Navab, Peter A.
Smethurst (Cambridge, Hinxton, United Kingdom; Munich, Germany)
P31
Desmopressin treatment improves whole-blood thrombus formation in patients
undergoing cardiothoracic surgery
Frauke Swieringa, Gerhardus J.A.J.M Kuiper, Marcus D. Lancé, Lieke Verheijen, Kevin
Hughes, Marco Marcus, Johan W.M. Heemskerk, Paola E.J van der Meijden
(Maastricht, The Netherlands)
P32
Increased platelet reactivity and platelet-monocyte interaction in HIV: an
underestimated cardiovascular risk factor?
Quirijn de Mast, Anna Schonsberg, Till Mathan, Meta Michels, Rob Fijnheer, Philip de
Groot, André van der Ven (Nijmegen, Utrecht, The Netherlands)
P33
Antithrombotic actions of statins involve PECAM-1 signaling
Leonardo A Moraes, Chris I. Jones, Sakthivel Vaiyapuri, Parvathy Sasikumar, Neline
Kriek, Marfoua Ali, Tanya Sage, Jonathan M Gibbins (Reading, United Kingdom)
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P34
Predictive value of thromboelastography for 1-year outcome after ST-elevated
myocardial infarction
Thea C. Godschalk, Thomas O. Bergmeijer, Arnoud Löwik, Jurriën M. ten Berg, Chris
M. Hackeng (Nieuwegein, The Netherlands)
P35
Quantitative, sensitive and robust determination of ATP and ADP secretion from
human and mouse platelets by an HPLC assay
Jörg Geiger, Claudia Schütz, Stepan Gambaryan (Würzburg, Germany)
P36
P-selectin tests to monitor platelet function remotely in cardiac patients
Sue Fox, Yanushi D Wijeyeratne, Mark Thomas, Andrew Johnson, Jane May, Stan
Heptinstall (Nottingham, United Kingdom)
P37
Absence of role for myosin Va in regulating platelet function
Matthew T. Harper, Marion T.J. van den Bosch, Ingeborg Hers, Alastair W. Poole
(Bristol, United Kingdom)
P38
Dual regulation of CD84 by ADAM10 and calpain in platelets
Sebastian Hofmann, Timo Vögtle, Markus Bender, Stefan Rose-John, Bernhard
Nieswandt (Würzburg, Kiel, Germany)
P39
Calcium-dependent cyclic nucleotide regulation by two PDE1 isoforms in
platelets
Cathrin Horras, Sabine Gätzner, Sabine Herterich, Jörg Geiger (Würzburg,
Germany)
P40
Chronic estradiol treatment reduces platelet responses and protects mice from
thromboembolism through the haematopoietic estrogen receptor 
Marie-Cécile Valéra, Marie-Pierre Gratacap, Pierre Gourdy, Françoise Lenfant,
Cendrine Cabou, Celine E. Toutain, Marlene Marcellin, Nathalie Saint Laurent, Pierre
Sié, Michel Sixou, Jean-François Arnal, Bernard Payrastre (Toulouse, France)
P41
Pollution derived diesel exhaust nanoparticles and their effects on human
platelets
Antonia Solomon, Erica Smyth, Naeem Mitha, Simon Pitchford, Pradeep Luther,
Andrew Thorley, Terry Tetley, Michael Emerson (London, United Kingdom)
P42
A novel flow cytometry-based platelet aggregation assay
Iris M. De Cuyper, Marjolein Meinders, Edith van de Vijver, Dirk de Korte, Leendert
Porcelijn, Masja de Haas, Johannes A. Eble, Taco W. Kuijpers (Amsterdam, The
Netherlands; Frankfurt am Main, Germany)
P43
High plasma levels of prostaglandin E2 in cord blood affect newborn platelet
function
Axel Schlagenhauf, Hans-Jörg Leis, Bettina Leschnik, Wolfgang Muntean (Graz,
Austria)
P44
Investigating the mechanism of action of sildenafil on platelet function in vitro
and in vivo
Georgina Apostoli, Michael Emerson (London, United Kingdom)
P45
Mechanism and target of action of Phikud Navakot extracts on human platelet
P. Pradmeeteekul, W. Kaewprem, N.P. Morales, U. Sotanaphun, D. Meksuriyen, P.
Rojnuckarin, R. Luechapudiporn (Chulalongkorn, Mahidol, Nakhon Pathom, Thailand)
13
P46
Lower levels of coated-platelets can be increased by hydroxyurea treatment in
essential thrombocytopenia
Béla Nagy Jr, Gyula Reményi, Róbert Szász, Ildikó Beke Debreceni (Debrecen,
Hungary)
P47
Immobilized amyloid  peptide supports platelet adhesion
Ilaria Canobbio, Laura G. Di Pasqua, Silvia Catricalà, Cesare Balduini, Mauro Torti
(Pavia, Italy)
P48
Whole blood thrombin generation monitored with a calibrated automated
thrombogram-based assay
Marisa Ninivaggi, Rafael Apitz-Castro, Yesim Dargaud, Bas de Laat, H. Coenraad
Hemker, Theo Lindhout (Maastricht, The Netherlands; Lyon, France)
14
POSTER PRESENTATION SESSION B. Friday, 21th September 2012
P50
Platelet adhesive functions are differentially affected in patients with filamin A
mutations
Eliane Berrou, Frédéric Adam, Marilyne Lebret, Patricia Fergelot, Alexandre Kauskot,
Martine Jandrot-Perrus, Alan Nurden, Rémi Favier, Jean-Philippe Rosa, Cyril Goizet,
Paquita Nurden, Marijke Bryckaert (Kremlin Bicêtre, Paris, Bordeaux, Pessac, Villejuif,
France)
P51
Changes in platelet function during storage: evaluation of a flow cytometry
method for studies of platelet adhesion and activation
Anna Södergren, Nahreen Tynngård, Sofia Ramström (Linköping, Sweden)
P52
A novel real-time whole blood flow cytometric assay identifies a subpopulation of platelets that rapidly bind annexin-V upon stimulation
Chris I. Jones, Stephen F. Garner, Jonathan M. Gibbins (Reading, Cambridge, United
Kingdom)
P53
Beta2-glycoprotein I has a protective function in situations of hyper-active von
Willebrand factor: implications for TTP
Vivian X. Du, Gwen van Os, Johanna A. Kremer Hovinga, Ilze Dienava-Verdoold,
Jacques Wollersheim, Zaverio M. Ruggeri, Rob Fijnheer, Philip G de Groot, Bas de
Laat (Maastricht, Utrecht, Amsterdam, The Netherlands; La Jolla, USA)
P54
A role for histone deacetylases in the regulation of platelet function
Marfoua S. Ali, Sakthivel Vaiyapuri, Chris I. Jones, Jonathan M. Gibbins, Michael J. Fry
(Reading, Cambridge, United Kingdom)
P55
The potential antithrombotic properties of polyphenolic-polysaccharides
conjugates from plants of Asteraceae and Rosaceae families
Joanna Saluk, Michał Bijak, Izabela Pawlaczyk, Paweł Nowak, Roman Gancarz,
Barbara Wachowicz (Lodz, Wroclaw, Poland)
P56
Continuous platelet production in the bloodstream is regulated by von
Willebrand factor
Sonia Poirault-Chassac, Kim Anh Nguyen, Audrey Pietrzyk, Caterina Casari, Agnès
Veyradier, Cécile V. Denis, Dominique Baruch (Paris, Le Kremlin-Bicêtre, Clamart,
France)
P57
Defining the effects of latex nanoparticles and their physicochemical
properties on platelet function to assess the thrombotic risk from ambient
particulate matter
E. Smyth, A. Solomon, A. Thorley, T. Tetley, M. Emerson (London, United Kingdom)
P58
Role of AMPK signalling in human platelet aggregation
Marie-Blanche Onselaer, Stéphane Eeckhoudt, Luc Bertrand, Jean-Louis
Vanoverschelde, Cécile Oury, Christophe Beauloye, Sandrine Horman (Liège,
Brussels, Belgium)
P59
CLP36 is a negative regulator of GPVI signaling in platelets
Shuchi Gupta, Attila Braun, Martina Morowski, Thomas Premsler, Markus Bender,
Zoltan Nagy, Albert Sickmann, Heike Hermanns, Michael Bösl, Bernhard Nieswandt
(Würzburg, Dortmund, Martinsried, Germany)
15
P60
Effect of storage temperature on in vitro quality of platelet concentrates
Johan W Lagerberg, Lara A. de Laleijne-Liefting, Pieter F van der Meer, Dirk de Korte
(Amsterdam, The Netherlands)
P61
Glucose prevents inhibition of platelets caused by NOS activation
Gennadi Kobzar, Vilja Mardla, Nigulas Samel (Tallinn, Estonia)
P62
The JAK2V617F mutation causes an increase in platelet reactivity in a knock-in
mouse model of essential thrombocythaemia
Catherine M. Hobbs, Cedric Ghevaert, Sonia Severin, Jocelyn Auger, Alexandra
Mazharian, Cavan Bennett, Harriet Manning, Lauren Brain, Juan Li, Anthony R. Green,
Steve P. Watson (Cambridge, Birmingham, United Kingdom)
P63
In vivo models for evaluating mouse and human platelet aggregation
responses
Charalambos Tymvios, Lisa-Marie Holbrook, Christopher Moore, Antonia Solomon,
Michael Emerson (London, United Kingdom)
P64
Diving into murine fetal and adult megakaryopoiesis
M. Meinders, I.M. De Cuyper, T.K. van den Berg, L. Gutiérrez (Amsterdam, The
Netherlands)
P65
Thrombopoiesis is not impaired in dengue-induced thrombocytopenia
Amaylia Oehadian, Meta Michels, Pandji Irani Fianza, Quirijn de Mast, Rudi Wisaksana,
Bachti Alisjahbana, André van der Ven (Bandung, Indonesia; Nijmegen, The
Netherlands)
P66
The role of Eph-Ephrin signaling in platelet function
Rekha H. Rana, Tanya Sage, Parvathy Sasikumar, Sakthivel Vaiypapuri, Neline Kriek,
Jonathan M. Gibbins (Reading, United Kingdom)
P67
Paradoxical up-regulation of platelet activation by anti-platelet therapies as a
consequence of a disruption of P2Y-TP receptor cross-talk
M.I. Butler, R.J.P. Pope, D.A. Marsden, J.F. Barton, A.D. Mumford, A.W. Poole, T.W.
Johnson, S.J. Mundell (Bristol, United Kingdom)
P68
Effect of three-dimensional hydrogel scaffolds on megakaryocyte differentiation
Audrey Pietrzyk, Sonia Poirault-Chassac, Didier Letourneur, Catherine Le Visage,
Dominique Baruch (Paris, France)
P69
LASP-1 deficiency in mice leads to increased bleeding time and impaired platelet
activation
Cora Reiss, Stepan Gambaryan, Ulrich Walter, Elke Butt (Würzburg, Germany)
P70
Expression of homopentameric and heteropentameric acetylcholine receptors in
megakaryocytes derived from CD34+ progenitor cells
Angelika Schedel, Kerstin Kaiser, Steffi Uhlig, Florian Lorenz, Karen Bieback, Peter
Bugert (Heidelberg, Mannheim, Germany)
P71
Favourable modulation of platelet function in female smokers by 12 weeks of
aerobic endurance training
Werner Schmid, Alice Assinger, Corinna Schlögl, Ivo Volf (Vienna, Austria)
P72
Rhinocetin, a venom-derived integrin-specific antagonist inhibits collagen
induced platelet and endothelial cell functions
16
Sakthivel Vaiyapuri, Marfoua S. Ali, Robert A. Harrison, Andrew B. Bicknell, Jonathan
M. Gibbins (Liverpool, Reading, United Kingdom)
P73
Genetic deletion of Vaccinia H1-related (VHR) phosphatase inhibits collageninduced platelet activation and thrombus formation without affecting bleeding
time in mice
Lucia Musumeci, Lisbeth Maurissen, Marijke J. Kuijpers, Fabio Cerignoli, Christelle
Lecut, William Guilmain, Marc Thiry, Yotis Senis, Michel Moutschen, Tomas Mustelin,
Lutz Tautz, Johan W.M. Heemskerk, Cécile Oury, Souad Rahmouni (Liège, Belgium;
Maastricht, The Netherlands; La Jolla, USA; Birmingham, United Kingdom)
P74
Activation of PAR4 by - and -thrombin is critically dependent on anion binding
exosite II
Niklas Boknäs, Maria Wallstedt, Lars Faxälv, Tomas Lindahl (Linköping, Sweden)
P75
Scott syndrome platelets demonstrate presence of TMEM16F-dependent and independent phospholipid scrambling in both platelet apoptosis and platelet
activation
Roger van Kruchten, Nadine J.A. Mattheij, Christine Saunders, Marion A.H. Feijge,
Peter W. Collins, Johan W.M. Heemskerk, Edouard M. Bevers (Maastricht, The
Netherlands; Cardiff, United Kingdom)
P76
Platelet flow-induced protrusion (FLIPR) formation leading to platelet
disintegration and procoagulant microparticles
C. Tersteeg, H.F. Heijnen, G. Pasterkamp, R.T. Urbanus, C. Maas, I. Hoefer, E.M.
Mak-Nienhuis, R. Nieuwland, F. Storti, F.N. van de Vosse, Ph.G. de Groot, M. Roest
(Utrecht, Amsterdam, Eindhoven, The Netherlands)
P77
Novel SNARE family members contribute to regulation of platelet granule
secretion
Ewelina M. Golebiewska, Robert A.N. Goggs, Christiane Wiegand, Gabriele Fischer
von Mollard, Alastair W. Poole (Bristol, United Kingdom; Bielefeld, Germany)
P78
Regulation of the guanine nucleotide exchange factor CalDAG-GEFI by protein
kinase A
Gianni Guidetti, Alessandra Consonni, Daria Manganaro, Ilaria Canobbio, Cesare
Balduini, Mauro Torti (Pavia, Italy)
P79
Platelet-associated tissue factor expression: shedding light on a controversial
issue
Laura Facchinetti, Daniela Boselli, Marta Brambilla, Paola Canzano, Elena Tremoli,
Marina Camera (Milano, Italy)
P80
Periodontopathogens induce expression of CD40L on human platelets via TLR2
and TLR4
Alice Assinger, Markus Laky, Sigrun Badrnya, Azadeh Esfandeyari, Ivo Volf (Vienna,
Austria; Stockholm, Sweden)
P81
Antiplatelet and antioxidative action of Trifolium pallidum and Trifolium scabrum
extracts
J. Kolodziejczyk-Czepas, B. Olas, J. Malinowska, B. Wachowicz, B. Szajwaj, I.
Kowalska, W. Oleszek, A. Stochmal (Lodz, Pulawy, Poland)
17
P82
Leukocyte-associated
immunoglobulin-like
receptor-1
deletion
in
megakaryocytes causes mild thombocytosis and formation of hyperactive
platelets
Alexandra Mazharian, Steven G. Thomas, Marie Lordkipanidze, Linde Meyaard, Yotis
A. Senis, Steve P. Watson (Birmingham, United Kingdom; Utrecht, The Netherlands)
P83
Murine platelet responsiveness is highly determined by its hyperlipidemic
environment
Marco van der Stoep, Menno Hoekstra, Ronald J. van der Sluis, Miranda van Eck,
Suzanne J.A. Korporaal (Leiden, Utrecht, Amsterdam, The Netherlands)
P84
Myosin IIA deficiency impairs granule distribution and actin dynamics in
megakaryocytes and platelets
Fabien Pertuy, Anita Eckly, Josiane Weber, François Lanza, Christian Gachet,
Catherine Léon (Strasbourg, France)
P85
Large-scale screening for extracellular protein interactions involved in platelet
aggregation
Yi Sun, Gavin J. Wright (Hinxton, United Kingdom)
P86
One hour resting effect on day 1 could be mimicked by 6 hours resting on 48
hours stored platelet concentrates by interruption of agitation at room
temperature in permeable bags
N.H. Timori, F.A. Soleimany, S.M.R. Tabatabai, Sh. Vaeli, B.A. Badlou (Zeist, The
Netherlands, Teheran, Iran)
P87
Endocannabinoids modulates the number of megakaryocytes in the bone
marrow
Patrizia Portanova, Sara Sampietro, Michela Nalin, Alessandra Bagarotti, Fabiola
Sinigaglia, Alessandra Bertoni (Novara, Italy)
P88
Identification of components of the blood coagulum according to SCHULTE
inducing proliferation of osteoblast-like cells
S. Krause, C. Linß, K. Vogel, J. Schubert, P. Presek (Halle-Wittenberg, Germany)
P89
Effects of cyclic nucleotides on CLEC-2-dependent platelet
Alessandra Borgognone, Steve P. Watson (Birmingham, United Kingdom)
P90
Would a new focus on vascular prostaglandins improve antiplatelet treatment
regimes?
Stan Heptinstall, David Iyú, Jacqueline R. Glenn, Ann E. White, Sue Fox (Nottingham,
United Kingdom)
P91
Targeting of type I protein kinase A to lipid rafts is required for competent
platelet inhibition by the cAMP-signaling pathway
Zaher Raslan, Ahmed Aburima, Simbareshe Magwenzi, Kjetil Tasken, Khalid M
Naseem (Hull, United Kingdom; Oslo, Norway)
P92
Deficiency of Src homology 2 domain-containing inositol 5-phosphatase 1
(SHIP1) affects membrane cytoskeleton organization and platelet internal
contraction
Sonia Séverin, Alessandra Consonni, Gaëtan Chicanne, Sophie Allart, Bernard
Payrastre, Marie-Pierre Gratacap (Toulouse, France; Pavia, Italy)
18
activation
P93
cAMP signaling pathway regulates platelet shape change through modulation of
RhoA activation and Rho Kinase-dependent signalling
Ahmed Aburima, Katie Wraith, Khalid. M. Naseem (Hull, United Kingdom)
P94
Shear-induced release of platelet receptors by metalloproteinases
Elizabeth Gardiner (Melbourne, Australia)
P95
Influence of the paraoxonase-1 Q192R genetic variant on clopidogrel
responsiveness and recurrent cardiovascular events: a systematic review and
meta-analysis
J.-L. Reny, C. Combescure, Y Daali, P. Fontana, for the PON1 meta-analysis group
(Geneva, Switzerland)
P96
The contribution of plasmatic proteins and SNPs to high biological variation in
the platelet proteome: a combined proteomic and genetic analysis
Michael Veitinger, Ellen Umlauf, Roland Baumgartner, Sigrun Badrnya, Christopher
Gerner, John Lamont, Rudolf Oehler, Maria Zellner (Crumlin, United Kingdom; Vienna,
Austria)
P97
Hypercholesterolemia is not necessarily associated with hyperreactive platelets
Suzanne J.A. Korporaal, Valentina de Angelis, Menno Hoekstra, Andrea Bochem,
Barbara Sjouke, Ronald J. van der Sluis, G. Kees Hovingh, Mark Roest, Miranda van
Eck (Leiden, Utrecht, Amsterdam, The Netherlands)
P98
Subtle role of platelet thrombospondin-1 interaction with CD36 in murine
thrombus formation on collagen
Marijke J.E. Kuijpers, Reyhan Nergiz-Unal, Susanne de Witt, Roger van Kruchten,
Suzanne J.A. Korporaal, Maria Febbraio, Marc F. Hoylaerts, Marc Tjwa,
Peter
J.
Voshol, Judith M.E.M. Cosemans, Johan W.M. Heemskerk (Maastricht, Leiden, The
Netherlands; Leuven, Belgium; Cleveland, USA; Frankfurt, Germany; Cambridge,
United Kingdom)
19