TRANSPORT OF AMINO ACIDS FROM BOVINE MILK

TRANSPORT OF AMINO ACIDS FROM BOVINE MILK WHEY THROUGH A CACO-2 CELLS
MONOLAYER
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Antonio José Goulart , Juliana Cristina Bassan , Eliana A. Varanda , Flávia Aparecida
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Resende , Ana Lúcia M. Nasser , Saulo S. Garrido , Reinaldo Marchetto , Rubens Monti . Univ
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Estadual Paulista – UNESP, Faculdade de Ciências Farmacêuticas. Rod. Araraquara-Jaú, Km
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1, Campus, 14801-902, Araraquara, SP, Brazil; Instituto de Química, Rua Prof. Francisco
Degni, 55, Quitandinha, 14800-900, Araraquara, SP, Brazil
Caco-2 cell was obtained from human colon adenocarcinoma and have the capacity to form
monolayers, simulating the intestinal epitelium. The objective of this research was to evaluate
the transport of bovine milk whey proteins hydrolysates through a Caco-2 cell monolayer.
Medium-grade of hydrolysis and high-grade of hydrolysis samples were produced adding
commercial proteases together or separately in the bovine whey, respectively. Caco-2 cells
monolayers was formed on inserts with collagen membrane; hydrolysates were added in the
apical side of the filters and were recovered in the basal side. Analysis by HPLC showed the
presence of low concentrations of amino acids in the basal side when the sample was nonhydrolyzed proteins (NH), expected for this sample, except Met. MGH presented leucine in a
concentration 28% greater than in NH sample, and 100% greater than in HGH sample. Tyrosine
was transported only from MGH sample at concentration of 8.284 nM; Met, Ile, Leu and Arg
were transported from all samples (NH – 488.481nM, 2.458nM, 8.606nM, 9.535nM; MGH –
488.019, 2.371nM, 11.005nM, 10.925nM; HGH – 468.040nM, 1.599nM, 5.336nM, 10.675nM,
respectivelly). Some other amino acids were not detected on the basal compartment even being
added in high concentrations in the apical side of the insert, demonstrating a selectivity exerted
by the cells. These results clearly suggests that different previous treatments of the samples
interfere in the transport and in the type of amino acids transported across the Caco-2 cells
monolayer.
Keywords: Caco-2 cells, bioavailability, whey protein hydrolysates
Supported by: CAPES/PNPD, CNPq, FUNDUNESP and FAPESP