Establishment of growth curve of Pfaffia
Transcrição
Establishment of growth curve of Pfaffia
Establishment of growth curve of Pfaffia glomerata (Spreng) root culture SILVA, T.F.O.1*; YAMAGUCHI, C.S.1; SINZKER, R.C.2; OLIVEIRA, A.J.B1,2; GONÇALVES, R.A.C.1,2 * 1Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá -PR, Brazil. 2 Departamento de Farmácia, Universidade Estadual de Maringá, Maringá-PR, Brazil *[email protected] Keywords: Pfaffia glomerata, root culture, metabolites. INTRODUCTION The Pfaffia glomerata (Spreng) Perderson, (Amaranthaceae) is popularly known as “Brazilian ginseng”. Their roots have bioactive compounds and ecdysterone has been described as the principal 1 constituent . The roots that are used in folk medicine as a tonic, aphrodisiac, and to treat gastric disturbance and are commercialized in Brazil as 2 substitutes for the Asian ginseng (Panax ginseng) . Chemical and pharmacological studies of this species are scarce compared the extent of their use. The propagation by cuttings was low multiplication, restricting its large-scale production for 2 pharmaceutical purposes . The establishment of growth curve of organs, such as roots, is essential due to the possible of identification the growth phases that happen importants metabolic 3,4 processes . The aim of this study was to determine the growth curve of P. glomerata root culture in liquid medium in the rotary shaker. RESULTS AND DISCUSSION In order to determine the growth curve of P. glomerata root cultures, 200mg of root from culture in vitro in solid MS medium (Murashige and Skoog, 1962) were inoculated into the 20mL of liquid medium MS and phytoregulator indole butyric acid -1 (0.3 mg L IBA) previously optimized. After 7 days, the tissues were weighed to determine the dry weight (DW). The growth curves were plotted according to DW of roots biomass from three replicates flasks that were harvested weekly for up to 8 weeks. According to Figure 1, we observed that the time required to obtain the best root growth is between four and five weeks (five times bigger than the initial biomass), after this there wasn´t significant growth, possibly due to beginning of cellular death. This phase of decline happen reduction of nutrients 3 and accumulation of toxic substances . Figure 1. Growth curve of Pfaffia glomerata root culture in liquid medium the according to DW of roots biomass. CONCLUSION In conclusion, it was possible to establish the growth curve of P. glomerata roots culture of in a -1 rotary shaker with 0,3 mg L IBA, the optimal growth was achieved is between four and five weeks. This technical can be used provides a rapid growth of plant material can be scale-up, in order to obtain primary and secondary metabolites of interest. ACKNOWLEDGEMENTS The authors are grateful to CAPES and PPG. REFERENCES 1. Moura, C. L. Avaliação da atividade antimicrobiana dos estratos brutos das espécies vegetais Miconia rubiginosa e Pfaffia glomerata em microrganismos da cavidade bucal. Mestrado, Universidade de Franca, Franca,SP, 2006. 2. Flores, R., et. al. Micropropagation and β-ecdysone content of the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa In Vitro Cell.Dev.Biol.—Plant , 2010, 46, 210–217. 3. Nogueira, R. C., et. al. Curva de crescimento e análises bioquímicas de calos de murici-pequeno (Byrsonima intermedia A. Juss.). Rev. Bras. Pl. Med., Botucatu, 2008, 10, 44-48 4. Saiman, M. Z.; Mustafa, N. R.; Schulte, A. E.; Verpoorte, R.; Choi, Y. H. Induction, characterization, and NMR-based metabolic profiling of adventitious root cultures from leaf explants of Gynura procumbens. Plant Cell Tiss Organ Cult, 2012, 109, 465–47 VII Workshop on Biocatalysis and Biotransformations and 1o Simposio Latinoamericano de Biocatalisis y Biotransformaciones