Biostock Sigma-Aldrich® 2011
Transcrição
Biostock Sigma-Aldrich® 2011
biomapping Genomics Biostock Sigma-Aldrich® 2011 Tudo Para a Sua Rotina da Molecular ! 2 biomapping Código Descrição Produto Preço (R$) PCR Quantitativo (DNA e RNA) D7440-100RXN Biostock Sigma-Aldrich JumpStart™ Taq ReadyMix™ for Quantitative PCR - 100 r 493.00 D7440-400RXN JumpStart™ Taq ReadyMix™ for Quantitative PCR - 400 r 1,589.00 L6419-100RXN LuminoCt® qPCR ReadyMix™ with Reference Dye - 100 r (Fast qPCR) 600.00 L6419-500RXN LuminoCt® qPCR ReadyMix™ with Reference Dye - 500 r (Fast qPCR) 2,799.00 QR0200 Quantitative RT-PCR ReadyMix - 100 r D6442-20RXN JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR - 20 r D6442-400RXN JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR - 400 r S4438-100RXN SYBR® Green JumpStart™ Taq ReadyMix™ for quantitative PCR - 100 r 493.00 S4438-500RXN SYBR® Green JumpStart™ Taq ReadyMix™ for quantitative PCR - 500 r 1,973.00 L6544-100RXN LuminoCt® SYBR® Green qPCR ReadyMix with Reference Dye - 100 r (Fast qPCR) 600.00 L6544-500RXN LuminoCt® SYBR® Green qPCR ReadyMix with Reference Dye - 500 r (Fast qPCR) 2,799.00 QR0100 SYBR® Green Quantitative RT-PCR Kit - 100 r S9194-20RXN SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR - 20 r S9194-400RXN SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR - 400 r 899.00 103.00 1,918.00 899.00 103.00 1,918.00 PCR e RT-PCR (enzimas e mixings) D1806-250UN Taq DNA Polymerase (with 10× buffer containing MgCl), rec - 250 u (5 u/µl) 110.00 D1806-1.5KU Taq DNA Polymerase (with 10× buffer containing MgCl), rec - 1.500 u (5 u/µl) 658.00 D4545-250UN Taq DNA Polymerase (with 10× buffer without MgCl), rec - 250 u (5 u/µl) 110.00 D4545-1.5KU Taq DNA Polymerase (with 10× buffer without MgCl), rec - 1.500 u (5 u/µl) 658.00 D8045-125UN AccuTaq™ LA DNA Polymerase (Long PCR - HiFi) - 125 u (5 u/µl) 260.00 D8045-500UN AccuTaq™ LA DNA Polymerase (Long PCR - HiFi) - 500 u (5 u/µl) 849.00 D9307-250UN JumpStart™ Taq DNA Polymerase - 250 u (2,5 u/µl) 151.00 D4184-250UN JumpStart™ Taq DNA Polymerase without MgCl - 250 u (2,5 u/µl) 151.00 D5809-500UN JumpStart™ AccuTaq™ LA DNA Polymerase - 500 u (2,5 u/µl) 849.00 D4309-250UN REDTaq® DNA Polymerase - 250 u (1 u/µl) 140.00 D8187-250UN JumpStart™ REDTaq® DNA Polymerase - 250 u (1 u/µl) 260.00 D1313-250UN JumpStart™ REDAccuTaq™ LA DNA Polymerase - 250 u (1 u/µl) 590.00 P4600-100RXN ReadyMix™ Taq PCR Reaction Mix with MgCl (50 µl volume final) 370.00 P2893-100RXN JumpStart™ Taq ReadyMix (50 µl volume final) 384.00 R2523-100RXN REDTaq® ReadyMix™ PCR Reaction Mix (50 µl volume final) 370.00 P0982-100RXN JumpStart™ REDTaq® ReadyMix™ PCR Reaction Mix (50 µl volume final) 399.00 M1302-40KU M-MLV Reverse Transcriptase - 40.000 u (200 u/µl) A4464-1KU Enhanced Avian Reverse Transcriptase - eAMV™ (20 u/µl; 50 reações) 1,020.00 STR1-1KT Enhanced Avian First Strand Synthesis Kit (20 µl volume final; 50 reações) 1,063.00 HSRT100-1KT Enhanced Avian HS RT-PCR Kit - 100 reações 2,125.00 O4387-.1ML Oligo(dT)23, Anchored 70 μM in H2O (0,5 ug/µl; 100 µl) 671.00 R7647-100UL Random Nonamers 50 μM in H2O (100 µl; 100 r) 288.00 R2520-2.5KU Ribonuclease inhibitor human (30.000 - 50.000 u/ml) - 2.500 u 300.00 R2520-10KU Ribonuclease inhibitor human (30.000 - 50.000 u/ml) - 10.000 u 990.00 DNTP10-1KT Deoxynucleotide Set (dATP, dCTP, dGTP, dTTP), 10 mM - 500 µl cada 301.00 DNTP100-1KT Deoxynucleotide Set (dATP, dCTP, dGTP, dTTP), 100 mM - 250 µl cada 603.00 753.00 3 Código Descrição Produto D7295-.2ML Deoxynucleotide Mix, 10 mM - PCR Reagent - 200 ul (1 ul/PCR 50 ul vol final) Preço (R$) 153.00 D7295-.5ML Deoxynucleotide Mix, 10 mM - PCR Reagent - 500 ul (1 ul/PCR 50 ul vol final) 310.00 A7721-200TST JumpStart™ Taq Antibody - 80 ul 864.00 B0300-1VL Betaine solution 5 M, PCR Reagent - 1,5 ml 95.00 D9170-1VL Dimethyl sulfoxide, PCR Reagent (1-10 %) - 1 ml 71.00 G8778-1VL Glycerol, PCR Reagent (5-10%) - 1,5 ml 74.00 Extração e amplificação XNAB2-1KT Extract-N-Amp™ Blood PCR Kit - 100 extrações / 100 amplificações 788.00 XNAP2-1KT Extract-N-Amp™ Plant PCR Kit - 100 extrações / 100 amplificações 863.00 XNAT2-1KT Extract-N-Amp™ Tissue PCR Kit - 100 extrações / 100 amplificações 825.00 E3004-1.2ML Extract-N-Amp™ PCR ReadyMix™ - 100 amplificações 463.00 XNAB-1KT REDExtract-N-Amp™ Blood PCR Kit - 100 extrações / 100 amplificações 884.00 XNAP-1KT REDExtract-N-Amp™ Plant PCR Kit - 100 extrações / 100 amplificações 861.00 XNAT-1KT REDExtract-N-Amp™ Tissue PCR Kit - 100 extrações / 100 amplificações 927.00 R4775-1.2ML REDExtract-N-Amp™ PCR ReadyMix™ - 100 amplificações 459.00 XNATG-1KT SYBR® Green Extract-N-Amp™ Tissue PCR Kit - 100 extrações / 100 amplificações 927.00 XNAPG-1KT SYBR® Green Extract-N-Amp™ Plant PCR Kit - 100 extrações / 100 amplificações 861.00 S4320-1.2ML SYBR® Green Extract-N-Amp™ PCR ReadyMix™ - 100 amplificações 466.00 Amplificação Genômica WGA2-10RXN GenomePlex® Complete Whole Genome Amplification (WGA) Kit - 10 reações WGA4-10RXN GenomePlex® Single Cell Whole Genome Amplification Kit - 10 reações WTA2-10RXN TransPlex® Complete Whole Transcriptome Amplification Kit - 10 reações 374.00 623.00 1,976.00 Purificação de RNA (total, mRNA e miRNA) R0901-100ML RNAlater® 428.00 T9424-25ML TRI Reagent® - 25 ml 213.00 T9424-100ML TRI Reagent® - 100 ml 493.00 T3809-25ML TRI Reagent® BD - 25 ml 226.00 T3809-100ML TRI Reagent® BD - 100 ml 522.00 T3934-25ML TRI Reagent® LS - 25 ml 299.00 T3934-100ML TRI Reagent® LS - 100 ml 690.00 RTN10-1KT GenElute™ Mammalian Total RNA Miniprep Kit - 10 preps 207.00 RTN70-1KT GenElute™ Mammalian Total RNA Miniprep Kit - 70 preps 1,090.00 MRN10-1KT GenElute™ mRNA Miniprep Kit - 10 preps 536.00 MRN70-1KT GenElute™ mRNA Miniprep Kit - 70 preps 2,670.00 DMN10-1KT GenElute™ Direct mRNA Miniprep Kit - 10 preps 550.00 DMN70-1KT GenElute™ Direct mRNA Miniprep Kit - 70 preps 2,993.00 SNC10-1KT mirPremier® microRNA Isolation Kit - 10 preps 230.00 SNC50-1KT mirPremier® microRNA Isolation Kit - 50 preps 961.00 STRN10-1KT Spectrum™ Plant Total RNA Kit - 10 preps 353.00 STRN50-1KT Spectrum™ Plant Total RNA Kit - 50 preps 880.00 R2020-250ML RNaseZAP® 205.00 AMPD1-1KT DNase I Amplification Grade - 1000 u (1 u/ul) 230.00 D5758-5ML Diethyl-pyrocarbonate (DEPC) 161.00 D5758-25ML Diethyl-pyrocarbonate (DEPC) 477.00 R8508-1ML tRNA from baker's yeast (S. cerevisiae), 10 mg/ml - 1 ml 243.00 4 biomapping Código Descrição Produto Preço (R$) DNA Plasmidial PFM50-1KT GenElute™ Five-Minute Plasmid Miniprep Kit - 50 preps Biostock Sigma-Aldrich 260.00 PFM250-1KT GenElute™ Five-Minute Plasmid Miniprep Kit - 250 preps NA0150-1KT GenElute™ HP Plasmid Miniprep Kit - 70 preps 1,170.00 NA0160-1KT GenElute™ HP Plasmid Miniprep Kit - 350 preps NA0200S-1KT GenElute™ HP Plasmid Midiprep Kit - 4 preps 353.00 NA0200-1KT GenElute™ HP Plasmid Midiprep Kit - 25 preps 753.00 NA0300S-1KT GenElute™ HP Plasmid Maxiprep Kit - 4 preps 394.00 NA0300-1KT GenElute™ HP Plasmid Maxiprep Kit - 10 preps 970.00 NA0310-1KT GenElute™ HP Plasmid Maxiprep Kit - 25 preps 1,700.00 NA0500-1KT GenElute™ HP Plasmid Megaprep Kit - 5 preps 1,281.00 PLED35-1KT GenElute™ Endotoxin-free Plasmid Midiprep Kit - 35 preps 1,209.00 NA0400S-1KT GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit - 4 preps NA0400-1KT GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit - 10 preps 990.00 NA0410-1KT GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit - 25 preps 1,955.00 NA0600-1KT GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit - 5 preps 1,426.00 NA0100-1KT PhasePrep™ BAC DNA Kit 1,602.00 449.00 1,990.00 453.00 DNA Genômico e Fragmentos NA2010-1KT GenElute™ Blood Genomic DNA Kit - 70 preps NA2020-1KT GenElute™ Blood Genomic DNA Kit - 350 preps 511.00 G1N70-1KT GenElute™ Mammalian Genomic DNA Miniprep Kit - 70 preps G1N350-1KT GenElute™ Mammalian Genomic DNA Miniprep Kit - 350 preps G2N70-1KT GenElute™ Plant Genomic DNA Miniprep Kit - 70 preps G2N350-1KT GenElute™ Plant Genomic DNA Miniprep Kit - 350 preps NA2110-1KT GenElute™ Bacterial Genomic DNA Kit - 70 preps NA2120-1KT GenElute™ Bacterial Genomic DNA Kit - 350 preps NA1111-1KT GenElute™ Gel Extraction Kit - 70 preps 438.00 NA1020-1KT GenElute™ PCR Clean-Up Kit - 70 preps 440.00 P1037-100G Phenol for molecular biology - 100 g 250.00 P1037-500G Phenol for molecular biology - 500 g 504.00 P4557-100ML Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA - 100 ml 230.00 P4557-400ML Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA - 400 ml 463.00 P4682-100ML Phenol solution Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2 - 100 ml 230.00 P4682-400ML Phenol solution Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2 - 400 ml 604.00 P3803-100ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE - 100 ml 282.00 P3803-400ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE - 400 ml 843.00 P2069-100ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE (w/buffer) - 100 ml 306.00 P2069-400ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE (w/buffer) - 400 ml 920.00 25666-100ML Chloroform – isoamyl alcohol mixture Fluka, 24:1, ≥ 99.5% - 100 ml 235.00 C2432-25ML Chloroform contains amylenes as stabilizer, ≥99% - 25 ml I9516-25ML 2-Propanol for molecular biology, ≥99% - 25 ml 89.00 I9516-500ML 2-Propanol for molecular biology, ≥99% - 500 ml 183.00 E7023-500ML Ethanol, absolute, 100 % (200 proof ) - 500 ml 183.00 2,413.00 511.00 2,413.00 685.00 2,740.00 671.00 2,422.00 85.00 5 Código Descrição Produto E7148-500ML Ethanol, absolute, 95 % (190 proof ) - 500 ml Preço (R$) 134.00 G3272-25G Guanidine hydrochloride, ≥99% - 25 g 102.00 G3272-100G Guanidine hydrochloride, ≥99% - 100 g 190.00 G9277-100G Guanidine thiocyanate, ≥99% - 100 g 246.00 G9277-250G Guanidine thiocyanate, ≥99% - 250 g 485.00 50985-50ML Guanidine thiocyanate solution Fluka, 4 M in H2O - 50 ml 270.00 P2308-25MG Proteinase K, ≥30 units/mg protein, lyophilized - 25 mg 261.00 P2308-100MG Proteinase K, ≥30 units/mg protein, lyophilized - 100 mg 439.00 P4850-1ML Proteinase K, >800 u/ml, buffered aqueous glycerol solution - 1 ml 257.00 L3790-10X1ML Lysozyme from chicken egg white, 10 mg/mL - 10 x 1 ml 330.00 L2524-10KU Lyticase from Arthrobacter luteus, lyophilized powder, ≥2,000 u/mg protein 230.00 G1767-1VL Glycogen from Mytilus edulis (Blue mussel), 20 mg/ml - 1 ml 931.00 P5413-500G Polyethylene Glycol, wt 8000 - 500 g 162.00 P5413-1KG Polyethylene Glycol, wt 8000 - 1 kg 246.00 R6513-10MG Ribonuclease A (from Pancreas Bovine), lyophilized - 10 mg 120.00 R6513-50MG Ribonuclease A (from Pancreas Bovine), lyophilized - 50 mg 387.00 R4642-10MG Ribonuclease A (from Pancreas Bovine), buffered aqueous glycerol solution - 10 mg 89.00 Marcadores Moleculares e Ladders de DNA P1598-40UG 20 bp Low Ladder - 80 pistas 377.00 S7025-50UG Step Ladder, 50 bp - 100 pistas 396.00 P1473-1VL 100 bp Ladder - 375 ul (75 pistas) 585.00 D5042-.1MG 123 bp Ladder - 100 pistas 685.00 D0428-1VL 1 kb Ladder - 100 pistas 590.00 D9780-20UG Lambda DNA Hind III Digest - 100 pistas 172.00 D0672-20UG φX174 DNA Marker Hae III Digest - 80 pistas 403.00 D7058-1VL DirectLoad™ Wide Range DNA Marker; 50 - 10.000 bp; 16 bandas (100 pistas) 521.00 D3812-1VL DirectLoad™ Step Ladder, 50 bp; 50 - 3.000 bp; 17 bandas (100 pistas) 352.00 D3687-1VL DirectLoad™ 100 bp Ladder; 100 - 1.000 bp; 10 bandas (75 pistas) 662.00 D3937-1VL DirectLoad™ 1 kb DNA Ladder; 500 - 10.000 bp; 11 bandas (100 pistas) 690.00 P9577-1VL PCR Marker solution; 50 - 2.000 bp; 8 bandas; w/loading for samples (50 pistas) 641.00 D2291-.5ML Pulse Marker™ 0.1-200 kb - 25 aplicações 266.00 D2416-.5ML Pulse Marker™ 50-1,000 kb - 25 aplicações 266.00 D4658-.5ML Pulse Marker™ 225-2,200 kb - 25 aplicações 275.00 Marcadores Moleculares de RNA* R6895-50UG Transcript RNA Markers 0.1-1 kb - 25 aplicações 566.00 R7020-50UG Transcript RNA Markers 0.2-10 kb - 25 aplicações 566.00 R7644-1VL Transcript RNA Markers 0.28-6.6 kb - 25 aplicações 618.00 Marcadores Moleculares de Proteínas C1992-1VL ColorBurst™ Electrophoresis Marker mol wt 8,000-220,000 Da - 500 µl 664.00 C4861-1VL ColorBurst™ Electrophoresis Marker High Range, mo w 30,000-220,000 Da - 500 µl 802.00 C6210-1VL Color Marker Ultra-low Range, mol wt 1,060-26,600 Da - 200 µl 477.00 C3187-.5ML Color Marker Low Range, mol wt 6,500-45,000 Da - 500 µl 629.00 SDS7B2-1VL Prestained Molecular Weight Marker mol wt 26,600-180,000 Da - 160 aplicações 558.00 M3913-1VL SigmaMarker™ Low Range, mol wt 6,500-66,000 Da (100 µl após reconstituição) 198.00 S8445-1VL SigmaMarker™ Wide Range, mol wt 6,500-200,000 Da (100 µl após rec) 249.00 S8320-1VL SigmaMarker™ High Range, mol wt 36,000-200,000 Da (100 µl após rec) 199.00 6 biomapping Código Descrição Produto M3546-1VL Ultra-low Range Molecular Weight Marker, mol wt 1,060-26,600 Da - 200 µl SDS7-1VL Molecular Weight Marker, mol wt 14,000-66,000 Da (1,5 ml após rec) Biostock Sigma-Aldrich Preço (R$) 629.00 244.00 SDS6H2-1VL Molecular Weight Marker, mol wt 30,000-200,000 Da (1,5 ml após rec) 224.00 I3018-1VL IEF Marker mix, PI 3.6-9.3 (250 µl após rec) 528.00 Corantes e Intercalantes B2025-1EA Brilliant Blue G - Colloidal Concentrate (1 L após diluição) 224.00 B8522-1EA Brilliant Blue G - Solution Concentrate (1 L após diluição) 231.00 B8647-1EA Brilliant Blue R - Concentrate, SDS-PAGE (1 L após diluição) 187.00 G1041-500ML EZBlue™ Gel Staining Reagent - 500 ml 263.00 P7170-1L Ponceau S solution - 1 L 283.00 S4942-200ML SYPRO® Ruby Protein Gel Stain (2D) - 200 ml 391.00 01494-500UL Nancy-520, 5,000 ×, 0,5 ml (limite detecção > 0,5 ng DNA) 419.00 E1510-10ML Ethidium bromide solution, 10 mg/mL, 10 ml ( > 1 ng DNA) 160.00 E1385-5ML Ethidium bromide solution, 500 μg/mL, 5 ml ( > 1 ng DNA) E7637-1G Ethidium bromide powder, 1 g ( > 1 ng DNA) S9430-.5ML SYBR® Green I nucleic acid gel stain, 10,000x in DMSO, 0,5 ml (> 60 pg DNA) 1,224.00 T8935-1L BlueView™ Nucleic Acid Stain, powder (1 L in 10x TAE buffer); > 200 ng DNA 256.00 T9060-1L BlueView™ Nucleic Acid Stain, powder (1 L in 10x TBE buffer); > 200 ng DNA 268.00 G2526-5ML Gel Loading Buffer (w/Bromophenol blue 0.05% w/v), 5 ml 72.00 G7654-5ML Gel Loading Solution (w/Br blue 0.25% w/v + Xylene cyanole 0.25% w/v), 6x conc 72.00 80.00 134.00 Enzimas de Modificação D2886-100UN T4 DNA Ligase - 100 u 199.00 D2886-500UN T4 DNA Ligase - 500 u 941.00 D0410-50UN T4 DNA Polymerase - 50 u 342.00 D0410-100UN T4 DNA Polymerase - 100 u 555.00 P4390-100UN T4 Polynucleotide Kinase - 100 u 300.00 P4390-500UN T4 Polynucleotide Kinase - 500 u 990.00 R6501-1VL Ribonuclease H - 30 u 588.00 P4978-1KU Phosphatase Alkaline (from calf intestine) - 1.000 u 398.00 R6142-100UN Acc I - 100 u 484.00 R6885-500UN Alu I - 500 u 310.00 R4258-2KU Apa I - 2.000 u 123.00 R6004-100UN Ava II - 100 u 222.00 R0260-1KU BamH I - 1.000 u R8631-500UN Bcl I - 500 u R6753-250UN Bgl I - 250 u 52.00 R6377-500UN Bgl II - 500 u 173.00 R4253-500UN BstE II - 500 u 260.00 R1761-1KU Cfo I - 1.000 u 272.00 Enzimas de Restrição 76.00 209.00 7 Código Descrição Produto R7763-500UN Cla I - 500 u Preço (R$) 399.00 R4256-200UN Dde I - 200 u 145.00 R4381-2KU Dra I - 2.000 u 336.00 R4640-5KU EcoR I - 5.000 u 94.00 R5628-1KU Hae III - 1.000 u 125.00 R1137-5KU Hind III - 5.000 u 109.00 R0629-500UN Hpa II - 500 u 189.00 R1258-1KU Kpn I - 1.000 u 118.00 R1507-50UN MluN I - 50 u 448.00 R4506-2KU Msp I - 2.000 u 295.00 R8761-100UN Nco I - 100 u 176.00 R5509-200UN Nde I - 200 u 220.00 R8506-200UN Not I - 200 u 282.00 R7023-500UN Pst I - 500 u 26.00 R1508-50UN Pvu I - 50 u 222.00 R2631-1KU Pvu II - 1.000 u 191.00 R4756-1KU Rsa I - 1.000 u 237.00 R5268-1KU SAC™ I - 1.000 u 248.00 R0754-1KU Sal I - 1.000 u 150.00 R0762-100UN Sau3A I - 100 u 197.00 R5007-1KU Sca I - 1.000 u 332.00 R4503-500UN Sma I - 500 u 164.00 R5257-150UN Spe I - 150 u 314.00 R7135-100UN Sph I - 100 u 188.00 R6759-200UN Ssp I - 200 u 243.00 R9507-2KU Taq I - 2.000 u 124.00 R7260-2KU Xba I - 2.000 u 300.00 R6379-2.5KU Xho I - 2.500 u 160.00 Membranas para Blotting 15356-1EA Blotting-Nylon 66 membranes, positive, roll, 0,2 m x 3 m N7892-5EA Nitrocellulose membranes, pore size 0.2 μm, 15 cm × 15 cm N8267-5EA Nitrocellulose membranes, pore size 0.45 μm, 15 cm × 15 cm P2938-1ROL Immobilon®-P PVDF membranes, roll, 0,265 m × 3,75 m 1,142.00 352.00 352.00 1,377.00 Anticorpos Secundários Conjugados e Substratos A4187-.25ML Anti-Goat IgG – Alk Phosphatase, produced in rabbit - 250 ul 268.00 A5420-1ML Anti-Goat IgG – Peroxidase, produced in rabbit - 1000 ul 580.00 F7367-.5ML Anti-Goat IgG – FITC, produced in rabbit - 500 ul 233.00 C2821-1ML Anti-Goat IgG – Cy3, produced in rabbit - 1000 ul 509.00 T7028-1ML Anti-Goat IgG – TRITC, produced in rabbit - 1000 ul 778.00 A3562-.25ML Anti-Mouse IgG − Alk Phosphatase, produced in goat - 250 ul 282.00 A9044-2ML Anti-Mouse IgG – Peroxidase, produced in rabbit - 2000 ul 816.00 F0257-.5ML Anti-Mouse IgG – FITC antibody produced in goat - 500 ul 207.00 C2181-1ML Anti-Mouse IgG – Cy3, produced in sheep - 1000 ul 999.00 T5393-.5ML Anti-Mouse IgG −TRITC, produced in goat - 500 ul 317.00 A3687-.25ML Anti-Rabbit IgG – Alk Phosphatase, produced in goat - 250 ul 256.00 A0545-1ML Anti-Rabbit IgG – Peroxidase, produced in goat - 1000 ul 605.00 F0382-.5ML Anti-Rabbit IgG – FITC, produced in goat - 500 ul 206.00 8 biomapping Código Descrição Produto T6778-1ML Anti-Rabbit IgG – TRITC, produced in goat - 1000 ul Preço (R$) 805.00 B1911-100ML BCIP®/NBT Liquid Substrate System - 100 ml Biostock Sigma-Aldrich 220.00 B6404-100ML BCIP®/NBT solution, premixed - 100 ml 215.00 B5655-5TAB SIGMAFAST™ BCIP®/NBT tablet 147.00 B5655-25TAB SIGMAFAST™ BCIP®/NBT tablet 502.00 F4523-5SET SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablet 300.00 T0565-100ML 3,3’,5,5’-Tetramethylbenzidine (TMB) Liquid Substrate System 322.00 90101-5MG-F Ampliflu Red BioChemika, for fluorescence 471.00 D4418-5SET SIGMAFAST™ 3,3’-Diaminobenzidine tablet 185.00 D0426-5SET SIGMAFAST™ DAB with Metal Enhancer tablet 140.00 Clonagem, Expressão e Purificação de Proteínas Rec Vetores de Expressão E8158-10UG pFLAG-ATS™ Expression Vector - 10 µg (tac promoter) 718.00 E8408-10UG pFLAG-CTC™ Expression Vector - 10 µg (tac promoter) 725.00 P1118-10UG pT7-FLAG™-1 Expression Vector - 10 µg 903.00 P9743-10UG pT7-FLAG™-4 Expression Vector - 10 µg 879.00 E5530-10UG pTAC-MAT-Tag®-1 Expression Vector - 10 µg 845.00 E5405-10UG pTAC-MAT-Tag®-2 Expression Vector - 10 µg 845.00 E5780-10UG pT7-MAT-Tag®-1 Expression Vector - 10 µg 779.00 E5655-10UG pT7-MAT-Tag®-2 Expression Vector - 10 µg E7658-20UG p3XFLAG-CMV™-10 Expression Vector - 20 µg 1,747.00 E9783-20UG p3XFLAG-CMV™-9 Expression Vector - 20 µg 1,811.00 C5989-20UG pCMV-MAT-Tag®-FLAG®-1 Expression Vector - 20 µg 1,995.00 E7029-20UG pBICEP-CMV™-3 Expression Vector - 20 µg 2,067.00 T6699-20UG pPolh-MAT™-1 Transfer Vector, fusion proteins in insect cells - 20 µg 1,397.00 845.00 Meios de Cultura e seus Componentes L2897-250G LB Agar, Powder - 250 g 293.00 L2897-1KG LB Agar, Powder - 1 Kg 887.00 L3147-250G Luria Agar, Powder - 250 g 272.00 L3147-1KG Luria Agar, Powder - 1 Kg 821.00 Y1500-250G YPD Agar, Powder - 250 g 215.00 Y1500-1KG YPD Agar, Powder - 1 Kg 649.00 L3522-250G Luria Broth, Powder - 250 g 178.00 L3522-1KG Luria Broth, Powder - 1 Kg 542.00 L7658-6X500ML LB Broth EZMix™ Powder 139.00 T0918-250G Terrific Broth, Modified, Powder - 250 g 192.00 T0918-1KG Terrific Broth, Modified, Powder - 1 Kg 585.00 T9179-2X5L Terrific Broth, Modified EZMix™ Powder 994.00 Y1375-250G YPD Broth, Powder - 250 g 226.00 Y1375-1KG YPD Broth, Powder - 1 Kg 683.00 A5054-250G Select Agar - 250 g 362.00 A5054-1KG Select Agar - 1 Kg 990.00 9 Código Descrição Produto P0556-250G Bacteriological Peptone - 250 g Preço (R$) 290.00 P0556-1KG Bacteriological Peptone - 1 Kg 880.00 T9410-250G Tryptone - 250 g 259.00 T9410-1KG Tryptone - 1 Kg 782.00 Y0875-250G Select Yeast Extract - 250 g 290.00 Y0875-1KG Select Yeast Extract - 1 Kg 874.00 S1797-10X5ML SOC Medium 328.00 Antibióticos, Indutor, Substratos e Agentes Seletivos A0166-5G Ampicillin sodium salt, cell culture tested, powder K1377-1G Kanamycin sulfate, cell culture tested, powder 228.00 I6758-1G IPTG - 1 g 179.00 I6758-5G IPTG - 5 g 790.00 B9146-10MG X-Gal - 10 mg (100 mg / ml) 133.00 B9146-100MG X-Gal - 100 mg (100 mg / ml) B3928-5ML Blue-White Select™ Screening Reagent (40mg/ml X-Gal + 40mg/ml IPTG) S7313-100MG S-Gal® - 100 mg 365.00 F5879-100G Ferric Ammonium Citrate - 100 g 136.00 G8168-10ML G-418 disulfate salt solution, 50 mg/ml, cell culture tested 318.00 A1720-1G G 418 disulfate salt powder, cell culture tested 457.00 P8833-10MG Puromycin dihydrochloride, cell culture tested 153.00 P8833-25MG Puromycin dihydrochloride, cell culture tested 322.00 H3274-50MG Hygromycin B powder, cell culture and insect cell culture tested 180.00 90.00 408.00 1,013.00 Soluções de Lise Celular e Inibidores de Proteases C8740-10ML CelLytic™ B Cell Lysis Reagent, for bacterial cell lysis, 10x conc - 10 ml 245.00 C5236-25ML CelLytic™ IB Inclusion Body Solubilization Reagent - 25 ml 224.00 C2978-50ML CelLytic™ M Cell Lysis Reagent, for Mammalian cell - 50 ml 219.00 R0278-50ML RIPA Buffer - 50 ml 197.00 C2360-50ML CelLytic™ P Cell Lysis Reagent, for Plant - 50 ml 222.00 C4482-50ML CelLytic™ Y Cell Lysis Reagent, for Yeast - 50 ml 205.00 P2714-1BTL Protease Inhibitor Cocktail, lyophilized (100 ml após rec) 199.00 P9599-1ML Protease Inhibitor Cocktail for plant, DMSO solution - 1 ml 243.00 P8849-1ML Protease Inhibitor Cocktail for Histidine-tagged proteins, DMSO solution - 1 ml 243.00 P8215-1ML Protease Inhibitor Cocktail for fungal and yeast, DMSO solution - 1 ml 243.00 P8340-1ML Protease Inhibitor Cocktail for mammalian cell, DMSO solution - 1 ml 263.00 Purificação usando HIS-Select® P6611-5ML HIS-Select® Nickel Affinity Gel - 5 ml (15 mg/ml binding) 280.00 P6611-25ML HIS-Select® Nickel Affinity Gel - 25 ml 990.00 H8162-5ML HIS-Select® Cobalt Affinity Gel - 5 ml (15 mg/ml binding) H8162-25ML HIS-Select® Cobalt Affinity Gel - 25 ml H7787-10EA HIS-Select® Spin Columns - 10 colunas (150 ug / coluna) 464.00 H9913-1EA HIS-Select® iLAP® 5 mL Column (lysis and purification) - 2 mg / coluna 158.00 I0125-100G Imidazole Reagent - 100 g 347.00 1,191.00 92.00 Purificação usando FLAG® F7425-.2MG ANTI-FLAG® antibody, produced in rabbit - 200 ug 1,401.00 A2220-1ML ANTI-FLAG® M2 Affinity Gel 1,746.00 10 biomapping Código Descrição Produto F2426-1ML EZview™ Red ANTI-FLAG® M2 Affinity Gel M8823-1ML Anti-FLAG® M2 Magnetic Beads Biostock Sigma-Aldrich Preço (R$) 1,887.00 1,260.00 Remoção de Sítios de Fusão FLAG® e MAT E5144-50UN Enterokinase - 50 u PRKE-1KT Enterokinase Removal Kit - ( 1,5 ml Anti-Enterokinase-Agarose; 10 preps) 924.00 F9302-50UG Factor X Activated (Xa) 413.00 T7513-100UN Thrombin from bovine plasma, lyophilized 486.00 RECOMT-1KT Thrombin CleanCleave™ Kit ( 1 ml Thrombin-Agarose; 10 preps) 780.00 1,615.00 Análise Estrutural e Protein Arrays T6567-5X20UG Trypsin, Proteomics Grade, Dimethylated (modified) - 100 ug PP0100-1KT Trypsin Profile IGD Kit - 100 aplicações 222.00 TT0010-10EA Trypsin Spin Columns, Proteomics Grade - 10 colunas 1,139.00 UPS1-1KT Universal Proteomics Standard Set (48 proteins) 2,336.00 MSCAL1-1KT ProteoMass™ Peptide and Protein MALDI-MS Calibration Kit 1,555.00 82009-1KT-F Crystallization Basic Kit for Proteins 1,421.00 GRAA2-1KT Panorama® Antibody Microarray - Gene Regulation I Kit (112 proteins); 2 slides 2,862.00 CSAA1-1KT Panorama® Antibody Microarray - Cell Signaling Kit (224 proteins); 2 slides 4,015.00 857.00 Epigenética ( Imprinting Genômico ) MOD50-1KT Imprint® DNA Modification Kit - 50 r MDQ1-48RXN Imprint® Methylated DNA Quantification Kit 870.00 1,725.00 Reagentes Ultrapuros A9539-100G Agarose, Analytical Grade - 100 g 342.00 A9539-500G Agarose, Analytical Grade - 500 g 1,507.00 A9414-10G Agarose, Low Melting Point - 10 g 219.00 A4718-25G Agarose, High resolution - 25 g 547.00 A6013-100G Agarose Type I, Low EEO - 100 g 598.00 A6013-500G Agarose Type I, Low EEO - 500 g 1,740.00 A2929-25G Agarose, for pulsed field electrophoresis - 25 g T6066-100G Trizma® base - 100 g 80.00 T6066-500G Trizma® base - 500g 189.00 B6768-500G Boric acid - 500 g 123.00 B6768-1KG Boric acid - 1 kg 204.00 E5134-100G EDTA, disodium salt dihydrate - 100 g T1258-25G Trizma® acetate - 25 g 133.00 T1258-100G Trizma® acetate - 100 g 364.00 A6283-500ML Acetic acid - 500 ml 125.00 T9650-1L Tris Acetate-EDTA (TAE) buffer, 10x concentrate - 1 L 151.00 T4415-1L Tris-Borate-EDTA (TBE) buffer, 10x concentrate - 1 L 158.00 T5941-100G Trizma® hydrochloride - 100 g 68.00 T5941-500G Trizma® hydrochloride - 500 g 295.00 G7126-100G Glycine - 100 g 356.00 68.00 72.00 11 Código Descrição Produto G7126-500G Glycine - 500 g Preço (R$) 119.00 A9099-100G Acrylamide - 100 g 210.00 A9099-500G Acrylamide - 500 g 621.00 M2022-25G N,N’-Methylenebisacrylamide - 25 g 122.00 M2022-100G N,N’-Methylenebisacrylamide - 100 g 344.00 T7024-25ML N,N,N’,N’-Tetramethylethylenediamine (TEMED) - 25 ml 109.00 A9164-25G Ammonium persulfate - 25 g 72.00 A9164-100G Ammonium persulfate - 100 g 131.00 L3771-100G Sodium dodecyl sulfate (SDS) - 100 g 188.00 U5378-500G Urea - 500 g 137.00 U5378-1KG Urea - 1 kg 253.00 M7154-25ML 2-Mercaptoethanol - 25 ml 53.00 M7154-100ML 2-Mercaptoethanol - 100 ml 113.00 S3014-500G Sodium chloride, Molecular Grade - 500 g 123.00 S3014-1KG Sodium chloride, Molecular Grade - 1 kg 188.00 S7277-1KG Sodium bicarbonate, Molecular Grade - 1 kg 189.00 P9791-100G Potassium phosphate monobasic, Molecular Grade - 100 g C4036-50G Cesium chloride - 50 g 158.00 G5516-100ML Glycerol - 100 ml 128.00 G5516-500ML Glycerol - 500 ml 174.00 F9037-100ML Formamide - 100 ml 183.00 D4551-500ML N,N-Dimethylformamide - 500 ml 183.00 D9779-1G DL-Dithiothreitol (DTT) - 1 g 148.00 D9779-5G DL-Dithiothreitol (DTT) - 5 g 445.00 D8906-10G Dextran sulfate, sodium salt - 10 g 170.00 P1585-1MG Phorbol - 1 mg 323.00 P5413-500G Poly(ethylene glycol), PEG 8.000 - 500 g 162.00 P9416-100ML TWEEN® 20 - 100 ml T8787-100ML Triton® X-100 - 100 ml F2637-5G Ficoll® - 5 g 183.00 I8896-50ML IGEPAL® CA-630 (NP-40) - 50 ml 127.00 H4034-25G HEPES - 25 g 117.00 H4034-100G HEPES - 100 g 294.00 M3183-25G MOPS - 25 g 107.00 M3183-100G MOPS - 100 g 243.00 D2532-5ML Denhardt’s Solution 50x Concentrate - 5 ml 345.00 W4502-1L Water, Molecular Grade - 1 L 155.00 75.00 87.00 89.00 Maiores Informações: Pedidos/Atendimento ao Cliente (Novos pedidos e dúvidas sobre pedidos existentes) Web: sigma-aldrich.com Tel: (11) 3732-3100 (São Paulo, capital) 0800-701-7425 (demais localidades) Fax: (11) 5522-9895 Ou nosso Representante de Vendas em sua região. Produtos Sigma Life Science sob Encomenda (Oligos de DNA, sondas e siRNAs) Web: sigma-aldrich.com/oligos Tel: (11) 3732-3100 Fax: (11) 5522-9895 Ou nosso Representante de Vendas em sua região. Serviço Técnico (Questões técnicas sobre produtos) Web: sigma-aldrich.com/techservice Tel: (11) 3732-3100 Fax: (11) 5522-9895 World Headquarters 3050 Spruce St. St. Louis, MO 63103 (314) 771-5765 sigma-aldrich.com Safety-related Information sigma-aldrich.com/safetycenter Global Locations For a list of our global locations and contact details, visit sigma-aldrich.com/international ©2010 Sigma-Aldrich Co. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, FLUKA, and SUPELCO are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Sigma brand products are sold through Sigma-Aldrich, Inc. JumpStart, ReadyMix, AccuTaq, eAMV, GeneSnare, Extract-N-Amp, REDExtract-N-Amp, GenElute, Spectrum, PhasePrep, DirectLoad, Pulse Marker, ColorBurst, SigmaMarker, EZBlue, BlueView, SAC, SigmaFAST, pFLAG-ATS, pFLAG-CTC, pT7-FLAG, pTAC-MAT, p3XFLAG -CMV, pCMV-MAT, pBICEP-CMV, pPolh-MAT, EZMix, Blue-White Select, CelLytic, EZview, CleanCleave, and ProteoMass are trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. LuminoCt, REDTaq, mirPremier, BCIP, S-GAL, HIS-Select, iLAP, FLAG, ANTI-FLAG, Panorama, Imprint, and Trizma are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. SYBR and SYPRO are registered trademarks of Molecular Probes, Inc. GenomePlex and TransPlex are registered trademarks of Rubicon Genomics, Inc. RNAlater and RNaseZAP are registered trademarks of Ambion, Inc. TRI Reagent is a registered trademark of Molecular Reseach Center, Inc. Immobilon is a registered trademark of Millipore, Inc. TWEEN is a registered trademark of Croda International PLC. Triton is a registered trademark of Dow Chemical Co. Ficoll is a registered trademark of GE Healthcare. IGEPAL is a registered trademark of Rhodia Operations. 73679-510140 1080