efficacy of topical formulation containing pimenta

EFFICACY OF TOPICAL FORMULATION
CONTAINING PIMENTA
PSEUDOCARYOPHYLLUS EXTRACT AGAINST
UVB-INDUCED OXIDATIVE STRESS IN
HAIRLESS MICE SKIN
CAMPANINI M(1); IVAN A(1); MARTINEZ R(1); VICENTINI (F2); VILELA F(2); FONSECA M(2);
BARACAT M(1); VERRI JR W(3); CASAGRANDE R(1); GEORGETTI S(1)
(1)Departamento de Ciências Farmacêuticas, Universidade Estadual de Londrina, Londrina, Parana,
Brasil; (2)Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de
Ribeirão Preto/USP, Ribeirão Preto, São Paulo, Brasil; (3)Departamento de Ciências Patológicas,
Universidade Estadual de Londrina, Londrina, Parana, Brasil.
[email protected]
Keywords: Antioxidant, Extract, Topical formulation, UVB irradiation
The skin is constantly challenged by oxidative stress. Therefore, topical administration of
antioxidants provides an efficient manner to enrich the endogeneous cutaneous protection system.
The leaves of P. pseudocaryophyllus present polyphenolic compounds in high concentration which
suggests that it might have the ability to act as an antioxidant. This study was designed to examine
the therapeutic effects of two different topical formulations (F1-non-ionic emulsion with high lipid
content and F2-anionic emulsion with low lipid content) containing P. pseudocaryophyllus extract
(PPE) against oxidative damages caused by UVB exposure. Oxidative stress was induced in hairless
mice (Registered under the number CEEA 112/09, process n°34994/209) by UVB irradiation (4.14
J/cm2). Mice received topical treatment on the dorsal surface with 0.5 g of the each formulation. The
reduced glutathione (GSH) levels was determined by the 5,5’dithiobis (2 nitrobenzoic acid)
spectrophotometric assay. The ferric reducing antioxidant power (FRAP) and 2,2’-azinobis-(3-ethyl
benzothiazoline-6-sulphonic acid) (ABTS) assays were used to determine the skin antioxidant
capacity. In this study, UV-B irradiation induced a decrease of approximately 1.58, 1.84 and 2,14
fold of ABTS scavenging capacity, iron reducing power and GSH levels in untreated irradiated
animals and irradiated animals treated with control formulations, respectively. Both formulations
containing PPE inhibited this depletion, maintaining levels similar to control (non irradiated) group
in tests. To our knowledge, this is the first study to demonstrate the beneficial effectiveness of PPE
on UV-B-induced skin oxidative stress. Therefore, the present study suggests topical formulations
containing PPE as possible treatment of skin-oxidative stress, which merits further pre-clinical and
clinical investigation.
Acknowledgments: Universidade Estadual de Londrina, Fundação Araucária and CNPq.