Title Induction of Callus from Mistletoe and Interaction with its Host

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Title Induction of Callus from Mistletoe and Interaction with its Host
Title
Author(s)
Citation
Issue Date
URL
Induction of Callus from Mistletoe and Interaction with its
Host Cells
Fukui, Mitsue; Azuma, Jun-Ichi; Okamura, Keizo
京都大学農学部演習林報告 = BULLETIN OF THE KYOTO
UNIVERSITY FORESTS (1990), 62: 261-269
1990-12-20
http://hdl.handle.net/2433/191963
Right
Type
Textversion
Departmental Bulletin Paper
publisher
Kyoto University
2
6
1
I
n
d
u
c
t
i
o
no
fCaH
耶 f I 加E 路 s
t
l
e
t
o
e
a
n
dI
n
t
e
r
a
c
t
i
o
nw
i
t
hi
t
sH
o
s
tC
e
l
l
s
MitsueFUKUI,JUル i
c
h
iA
z
UMA,and K
eizo OKAMURA
ヤドリギ@カルスの誘導と
宿主細胞との栴互作用
福井充枝・東
1
員
一岡村
Resume
A 回l1u
s was i
n
d
u
c
e
d from m
i
s
t
l
e
t
o
e (Viscum album L
.v
a
rl
u
t
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s
c
e
n
s M.), a
nd
e
s
t
a
b
l
i
s
h
e
d on a m
o
d
i
f
i
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d Murashig
e
Skoog (MS) medium h
a
v
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a
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ft
h
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u
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h1
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. When t
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要
ヤドリギ (
Viscumαlbum L
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s M.) から、カイネチン1.0ppm,ナフタレン酢費量
1
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0
p
p
mを含む,イオン強度会半分にした M
u
r
a
s
h
i
g
e
S
k
o
o
g(MS) 改変培地を用い, 2
80C、 1
2
時間の明暗府知,光度約 2
,
5
0
0
1
u
xの条件下で、培謎し,カルスを誘導した。カルスは淡いピンク色
1
で、あった。また,プランテックスを用いて, 3週間措援した
で,その細胞の平均間後は約7.5μm
とき,その成長識が最大ζ
l述 し , 絶 乾 畿 が 約 3
{
告になった カルス細胞には,分子・議 7
7,
0
0
0、
1
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1
¥
O
された。一方、ヤドリギのカルスをブナのカルスに接触させたところ,拘カルスは接触 i
院議を起
さずに生長し統ける乙とがわかった。
1
.I
NTRODUCTION
P
l
a
n
tc
巴l
l
sa
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e known t
or
e
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o
g
n
i
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a
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ym
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a
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t and p
r
o
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c
tt
h
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m
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e
l
v
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n
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te
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r
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r
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o
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o
n
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e
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a
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fi
n
f
o
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a
t
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n
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e
yt
oc
l
a
r
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e
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o
g
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t
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ns
y
s
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e
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se
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c
t
e
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ob
eo
b
t
a
i
n
e
d from
2
6
2
a
r
a
s
i
t
ei
n
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e
r
a
c
t
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o
n
s
. 1
nt
h
ef
i
e
l
do
f woody p
l
a
n
t,
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h
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r
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ft
h
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th
o
s
tp
m
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t
sh
o
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ts
田 町1 t
o be an a
t
t
r
a
c
t
i
v
es
t
a
r
t
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n
gm
a
t
e
r
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o
rt
h
i
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i
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f
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v
e
s
t
i
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a
t
i
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n
. Althought
h
ep
h
y
s
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o
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o
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a
l
l
ya
c
t
i
v
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o
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t
so
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i
s
t
l
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o
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t
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u
t
r
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e
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t
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t
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s toward h
o
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t and anatomy o
fm
i
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t
l
e
t
o
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o
s
t were r
e
p
o
r
t
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dH 2), l
knowna
b
o
u
tc
e
l
l
c
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l
li
n
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e
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t
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o
nbetw配 nm
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s
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e
t
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eandi
t
sh
o
st.官時間f
o
r
e,i
nt
h
i
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t
u
d
y
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n
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n
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.
)andb
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h
(Fagus c
r
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a Bl
.
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nv
i
t
r
o
. F
i
r
s
t,we t
r
i
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n
d
u
c
ea c
a
l
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s from m
i
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e
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o
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c
o
n
f
i
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e
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h
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e
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to
fb
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grown i
nt
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附
2
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2
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幽
4
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