ana–maria chiorcea paquim

CURRICULUM VITAE
Name:
Born:
Institute address:
ANA–MARIA CHIORCEA PAQUIM
4 February 1975
Laboratório de Electroanálise e Corrosão, Instituto Pedro
Nunes, Rua Pedro Nunes, 3030-199 Coimbra, Portugal
+351 239 700 978
+351 239 700 965
[email protected]
Telephone:
Fax:
E-mail:
Research Interests
Nanoscience, nanotechnology, biosensors technology, biomaterials,
bioelectrochemistry.
Languages
Romanian, Portuguese, English, French, Spanish
Education
1993 – 1997
B.Sc. in Physics, Faculty of Physics, University of Bucharest, Romania.
1997 – 1999
M.Sc. in Biophysics, Department of Biophysics, Faculty of Physics,
University of Bucharest, Romania.
1999 – 2003
Ph.D. in Biochemistry, Department of Biochemistry, Faculty of Science and
Technology, University of Coimbra, Portugal.
Scientific Training
Scientific training that led to the elaboration of the B.Sc. Thesis, in the Laboratory of
Radiation Hygiene from the Public Health Institute, Bucharest, Romania, 1 November 1996 –
1 July 1997.
Scientific training that led to the elaboration of the M.Sc. thesis, in the Laboratory of
Electrochemistry from the Chemistry Department, Faculty of Science and Technology,
Coimbra, Portugal, 1 October 1998 – 30 June 1999.
SPQ–Analítica’99, Coimbra, Portugal, 21 – 22 October 1999.
Scientific training in the research group of Prof. Dr. Tibor Hianik, in the Laboratory of
Biophysics, Department of Biophysics and Chemical Physics, Faculty of Mathematics,
Physics and Informatics, Comenius University, Bratislava, Slovakia, 26 February – 15 March
2000.
Scientific training in the research group of Prof. Dr. Hans Siegenthaler, in the Laboratory of
Electrochemistry, Department of Chemistry and Biochemistry, University of Bern, Bern,
Switzerland, 5 – 20 February 2001.
2
IUPAC – Workshop on “Electrochemistry and interfacial chemistry in environmental cleanup and green processes”, Coimbra, Portugal, 6 – 7 April 2001.
Scientific training in European Office of Molecular Imaging Corporation, Witec GmbH, Ulm,
Germany, 14 – 19 July 2001.
Advanced Course NATO Advanced Study Institute, Scanning Probe Microscopy:
Characterization, Nanofabrication and Device Application of Functional Materials,
Albufeira, Portugal, 1 – 13 October 2002.
Seminar Biomedicina integrated in the Ciclo de Seminários INOVAÇÃO – Sáude, Instituto
Pedro Nunes, Coimbra, Portugal, April 16, 2003.
Seminar Indústria e Impacto Ambiental in the Ciclo de Seminários INOVAÇÃO – Ambiente,
Energia e Desenvolvimento, Instituto Pedro Nunes, Coimbra, Portugal, 28 May 2003.
Advanced Course Life Sciences Interface Spring School, University of Nottingham,
Nottingham, UK, 29 March – 2 April 2004.
Scientific training in the research group of Prof. Dr. Laura Tugulea, in the Laboratory of
Biophysics, Faculty of Physics, University of Bucharest, Bucharest, Romania, 16 – 30
November 2005.
Scientific training in the research group of Prof. Dr. Laura Tugulea, in the Laboratory of
Biophysics, Faculty of Physics, University of Bucharest, Bucharest, Romania, 16 – 30
December 2006.
Advanced Course Linz Hands-on Winter School, University of Linz, Linz, Austria, 31 January
– 2 February 2007.
Seminar “Preparação de amostras – Técnica de SPE”, Hucoa–Erloss®, Equipamentos
Científicos Lda., Instituto Pedro Nunes, Coimbra, Portugal, 12 June 2007.
Seminar “Análise Técnica de Colunas de HPLC”, Hucoa–Erloss®, Equipamentos Científicos
Lda., Instituto Pedro Nunes, Coimbra, Portugal, 11 July 2007.
Scientific training in the research group of Prof. Dr. Ramon Eritja, Institute for Research in
Biomedicine (IRB Barcelona), Barcelona, Spain, 25 – 30 January 2010.
Projects
Principal Investigator
“Nanostructured human telomeric DNA electrochemical biosensor for screening anti-cancer
drugs”, from 2012 to 2015, financed by FCT (PTDC/SAU-BMA/118531/2010).
Collaboration:
“On line electrochemical pathogen alarm based on DNA-hybridization (PATH-ALARM)”,
INCO-Copernicus, from 1999 to 2003, financed by European Union (ERBICT15CT960804).
3
“Development and medical applications of electrochemical DNA-biosensors”, from 1999 to
2001, financed by FCT (PRAXIS/C/SAU/93/96).
“Evaluation/Validation of novel biosensors in real environmental and food samples
(Partner)”, from 2000 to 2003, financed by European Union (QLK3-2000-01311)
“DNA-electrochemical biosensors-Surface characterization by scanning probe microscopy
(SPM)”, from 2003 to 2006, financed by FCT (POCTI/QUI/47201/2002)
“Nanobioelectrochemistry: development, surface characterization and applications of layer
by layer nanostrutured DNA- and enzyme-modified electrodes” from 2008 to 2010, financed
by FCT (PTDC/QUI/65255/2006).
“Novel strategies for electrode surface modification and adsorption: nanoscale
characterisation and application to sensor development and corrosion inhibition”, from 2009
to 2011, financed by FCT (PTDC/QUI/65732/2006).
Self-Assembled Guanosine Structures for Molecular Electronic Devices - European Network
on G-Quartets and Quadruplex Nucleic Acids (G4-NET) (Partner), from 2008 to 2012, COST
Action MP0802 (Materials, physical and nanosciences).
“Development, characterization and applications of DNA biosensors”, from 2010 to 2013,
financed by FCT (PTDC/QUI-QUI/098562/2008).
Development, characterization and applications of DNA biosensors, from 2010 to 2013,
financed from FCT (PTDC/SAU-BEB/104643/2008).
DNA-protein and DNA-cancer cell interactions characterized by AFM and electrochemistry,
from 2013 to 2010, financed from FCT (PTDC/QEQ-MED/0586/2012).
Theses
A.–M. Chiorcea (1997), Thermoluminescent dosimetry applied on medical radiography,
B.Sc. Thesis, Faculty of Physics, University of Bucharest, Bucharest, Romania.
A.–M. Chiorcea (1999), Imaging guanosine by scanning probe microscopy,
Master Thesis, Faculty of Physics, Department of Biophysics, University of Bucharest,
Bucharest, Romania.
A.–M. Chiorcea–Paquim (2003), Scanning probe microscopy of DNA and other biological
molecules adsorbed on the electrodes surfaces, Ph.D. Thesis, Department of Biochemistry,
Faculty of Science and Technology, University of Coimbra, Coimbra, Portugal.
4
Grants
1998 – 1999 Socrates–Erasmus fellowship, Laboratory of Electrochemistry and Corrosion,
Department of Chemistry, University of Coimbra, Portugal (9 months).
1999 – 2003 Ph.D. grant PRAXIS XXI/BD/19728/99, Fundação para a Ciência e
Tecnologia (48 months).
2002
NATO fellowship to attend the course NATO Advanced Study Institute,
Scanning Probe Microscopy: Characterization, Nanofabrication and Device
Application of Functional Materials, October 2002, Albufeira, Portugal.
2003 – 2006 Post-doctoral grant SFRH/BPD/14425/2003 from Fundação para a Ciência e
Tecnologia (36 months).
2006 – 2009 Post-doctoral grant SFRH/BPD/27087/2006, Fundação para a Ciência e
Tecnologia (30 months).
Publications
Book Chapters
1.
A.–M. Chiorcea, A.M. Oliveira Brett, AFM of guanine adsorbed on HOPG under
electrochemical control, ASI NATO on Scanning Probe Microscopy: Characterization,
Nanofabrication and Device Application of Functional Materials, Ed. Paula Vilarinho et
al., Kluwer Academic Publishers, Netherland, 2005, volume 467, pages 467 – 473.
2.
A.M. Oliveira Brett, V.C. Diculescu, A.–M. Chiorcea–Paquim, S.H.P. Serrano, DNA–
electrochemical biosensors for investigating DNA damage, Comprehensive Analytical
Chemistry, Electrochemical Sensor Analysis, Eds. S. Alegret and A. Merkoçi, 2007, Vol.
49, pages 413 – 437.
3.
A.M. Chiorcea Paquim, Ana M. Oliveira Brett, Procedure 28: Atomic force microscopy
characterization of a DNA electrochemical biosensor, Comprehensive Analytical
Chemistry, Electrochemical Sensor Analysis, Eds. S. Alegret and A. Merkoçi, 2007, Vol.
49, pages e203 – e205.
4.
A.M. Chiorcea Paquim, P. Santos,V.C. Diculescu, R. Eritja and A.M. Oliveira Brett,
Electrochemical characterization of guanine quadruplexes, Guanine quartets – structure
and application, Ed. G.P. Spada, 2013, pp. 100-109.
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Articles
1.
A.–M. Chiorcea, A.M. Oliveira Brett, Scanning probe microscopic imaging of guanine
on a highly oriented pyrolytic graphite electrode, Bioelectrochemistry, 2002, 55, 63 –
65.
2.
A.M. Oliveira Brett, J.A.P. Piedade, A.–M. Chiorcea, Anodic voltammetry and AFM
imaging of picomoles of adriamycin adsorbed onto carbon surfaces, Journal of
Electroanalytical Chemistry, 2002, 538–539, 267 – 276.
3.
A.M. Oliveira Brett, A.–M. Chiorcea, Effect of pH and applied potential on the
adsorption of DNA on highly oriented pyrolytic graphite electrodes. Atomic
force microscopy surface characterisation, Electrochemistry Communications, 2003, 5,
178 – 183.
4.
A.M. Oliveira Brett, A.–M. Chiorcea, Atomic force microscopy of DNA
immobilized onto a highly oriented pyrolytic graphite electrode surface, Langmuir,
2003, 19, 3830 – 3839.
5.
A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Microscopia de força atómica de
moléculas de DNA adsorvidas na superfície de HOPG, Química – Boletim da
Sociedade Portuguesa de Química, 2004, 94, 57 – 68.
6.
A.–M. Chiorcea, A.M. Oliveira Brett, Atomic force microscopy characterisation of an
electrochemical DNA–biosensor, Bioelectrochemistry, 2004, 63(1–2), 229 – 232.
7.
A.–M. Chiorcea–Paquim, V. Diculescu, A.T.S. Oretskaya, A.M. Oliveira Brett, AFM
and electroanalytical studies of synthetic oligonucleotide hybridization, Biosensors
and Bioelectronics, 2004, 20, 933 – 944.
8.
A.M. Oliveira Brett, A.–M. Chiorcea, DNA imaged on a HOPG electrode surface by
AFM with controlled potential, Bioelectrochemistry, 2005, 66, 117 – 124.
9.
A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, V. Diculescu, T.S. Oretskaya,
Synthetic oligonucleotides: AFM characterisation and electroanalytical studies,
Bioelectrochemistry, 2005, 67, 181 – 190.
10. V.A. Alves, A.–M. Chiorcea–Paquim, A. Cavaleiro, C.M.A. Brett, The nanostructure
and microstructure of steels: Electrochemical Tafel behaviour and atomic force
microscopy, Corrosion Science, 2005, 47, 2871 – 2882.
11. V.C. Diculescu, A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Electrochemical DNA
sensors for detection of DNA damage, Sensors, 2005, 5, 377 – 393.
12. A.–M. Chiorcea–Paquim, T.S. Oretskaya, A.M. Oliveira Brett, Adsorption of synthetic
homo– and hetero–oligodeoxynucleotides onto highly oriented pyrolytic graphite:
atomic force microscopy characterization, Biophysical Chemistry, 2006, 121, 131 –
141.
13. A.–M. Chiorcea–Paquim, T.S. Oretskaya, A.M. Oliveira Brett, Atomic force
microscopy characterization of synthetic pyrimidinic oligodeoxynucleotides
6
adsorbed onto an HOPG electrode under applied potential, Electrochimica Acta,
2006, 51, 5037 – 5045.
14. A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, V.C. Diculescu, J.A.P. Piedade,
Electrochemistry of nanoscale DNA surface films on carbon, Medical Engineering &
Physics, 2006, 28, 963 – 970.
15. R. Pauliukaite, C.M.A. Brett, A.–M. Chiorcea–Paquim, A.M. Oliveira Brett,
Electrochemical, EIS and AFM characterization of biosensors: Trioxysilane sol–gel
encapsulated glucose oxidase with two different redox mediators, Electrochimica
Acta, 2006, 52, 1 – 8.
16. A.–M. Chiorcea–Paquim, J.A.P. Piedade, R. Wombacher, A. Jäschke, A.M. Oliveira
Brett, Atomic force microscopy and anodic voltammetry characterization of a 49–
mer Diels–Alderase ribozyme, Analytical Chemistry, 2006, 78, 8256 – 8264.
17. V.C. Diculescu, A.M. Chiorcea–Paquim, O. Corduneanu, A.M. Oliveira Brett,
Palladium nanoparticles and nanowires electrochemically deposited: AFM and
electrochemical characterization, Journal of Solid State Electrochemistry, 2007, 11,
887 – 898.
18. D.P. Santos, M.V.B. Zanoni, M.F. Bergamini, A.–M. Chiorcea–Paquim, V.C. Diculescu,
A.M. Oliveira Brett, Poly(glutamic acid) nanofibre modified glassy carbon electrode:
Characterization by atomic force microscopy, voltammetry and electrochemical
impedance, Electrochimica Acta, 2008, 53, 3991 – 4000.
19. J. Martinovic, A.–M. Chiorcea–Paquim, V.C. Diculescu, J. Van Wyk, E. Iwuoha,
P. Bake, S. Mapolie, A.M. Oliveira Brett, Metallo–functionalized first–generation
salicylaldimine poly(propylenimine) tetraamine dendrimers: Electrochemical study
and atomic force microscopy imaging, Electrochimica Acta, 2008, 53, 4907 – 4919.
20. A.–M. Chiorcea–Paquim, R. Pauliukaite, C.M.A. Brett, A.M. Oliveira Brett,
AFM nanometer surface morphological study of in situ electropolymerized neutral
red redox mediator oxysilane sol–gel encapsulated glucose oxidase electrochemical
biosensors, Biosensors and Bioelectronics, 2008, 24, 297 – 305.
21. O. Corduneanu, V.C. Diculescu, A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Shapecontrolled palladium nanowires and nanoparticles electrodeposited on carbon
electrodes, Journal of Electroanalytical Chemistry, 2008, 624, 97 – 108.
22. A.–M. Chiorcea–Paquim, T.S. Oretskaya, A.M. Oliveira Brett, AFM characterization of
synthetic homo– and hetero–oligodeoxynucleotides adsorbed onto HOPG,
Proceedings of the IX. Linz Winter Workshop 2007: Advances in Single Molecule
Research for Biology and Nanoscience, Eds. Peter Hinterdorfer, Gerhard Schutz and
Peter Pohl, Johannes–Kepler–Universitat Linz, Linz, Austria, 2008, 43 – 48.
23. O. Corduneanu, A.–M. Chiorcea–Paquim, M. Garnett, A.M. Oliveira Brett, Lipoic acid–
palladium complex interaction with DNA, voltammetric and AFM characterization,
Talanta, 2009, 77, 1843 – 1853.
7
24. V.C. Diculescu, A.–M. Chiorcea–Paquim, L. Tugulea, M. Vivan, A.M. Oliveira Bret,
Interaction of imatinib with liposomes: Voltammetric and AFM characterization,
Bioelectrochemistry, 2009, 74, 278 – 288.
25. A.–M. Chiorcea–Paquim, O. Corduneanu, S.C.B. Oliveira, V.C. Diculescu,
A.M. Oliveira Brett, Electrochemical and AFM evaluation of hazard compounds–
DNA interaction, Electrochimica Acta, 2009, 54, 1978 – 1985.
26. S.C.B. Oliveira, A.M. Chiorcea-Paquim, S.M. Ribeiro, A.T.P. Melo, M. Vivan, A.M.
Oliveira-Brett, In situ electrochemical and AFM study of thalidomide–DNA
interaction, Bioelectrochemistry, 2009, 76, 201 – 207.
27. T.A. Enache, A. – M. Chiorcea – Paquim, O. Fatibello – Filho, A.M. Oliveira – Brett,
Hydroxyl radicals electrochemically generated in situ on a boron – doped diamond
electrode, Electrochemistry Communications, 2009, 11, 1342 – 1345.
28. O. Corduneanu, A.–M. Chiorcea – Paquim, S.M. Fiuza, M.P.M. Marques, A.M. Oliveira
– Brett, Polynuclear palladium complexes with biogenic polyamines: AFM and
voltammetric characterization, Bioelectrochemistry, 2010, 78, 97 – 105.
29. O. Corduneanu, A.–M. Chiorcea – Paquim, S.M. Fiuza, M.P.M. Marques, A.M. Oliveira
– Brett, DNA Interaction with Palladium Chelates of Biogenic Polyamines Using
Atomic Force Microscopy and Voltammetric Characterization, Analytical Chemistry,
2010, 82, 1245 – 1252.
30. V.C. Diculescu, A.–M. Chiorcea – Paquim, R. Eritja and A.M. Oliveira – Brett,
Thrombine Binding Aptamer Quadruplex Formation: AFM and Voltammetric
Characterization, Journal of Nucleic Acids, 2010, 2010, Article ID 841932, 8 pages.
31. V.C. Diculescu, A.M. Chiorcea Paquim, R. Eritja and A.M. Oliveira – Brett, Evaluation
of the structure–activity relationship of thrombin with thrombin binding aptamers
by voltammetry and atomic force microscopy, Journal of Electroanalytical Chemistry,
2011, 656, 159 – 166.
32. A.D. Rodrigues Pontinha, S.M. Alves Jorge, A.M. Chiorcea Paquim, V.C. Diculescu,
A.M. Oliveira Brett, In situ evaluation of anticancer drug methotrexate–DNA
interaction using a DNA-electrochemical biosensor and AFM characterization,
Physical Chemistry Chemical Physics, 2011, 13, 5227 – 5234.
33. T. Batista, A.M. Chiorcea Paquim, A.M. Oliveira Brett, C.C. Schmitt, M.G. Neumann,
Laponite
RD/polystyrenesulfonate
nanocomposites
obtained
by
photopolymerization, Applied Clay Science, 2011, 53, 27 – 32.
34. A.M. Chiorcea Paquim, P.V. Santos, R. Eritja, A.M. Oliveira Brett, Self-assembled Gquadruplex nanostructures: AFM and voltammetric characterization, Physical
Chemistry Chemical Physics, 2013, 15(23), 9117 – 9124.
35. A.M. Chiorcea Paquim, P.V. Santos, A.M. Oliveira Brett, Atomic force microscopy and
voltammetric
characterisation
of
synthetic
homo-oligodeoxynucleotides,
Electrochimica Acta, 2013, 110, 599 – 607.
8
36. A.M. Chiorcea Paquim, A.M. Oliveira Brett, Redox behavior of G-quadruplexes,
Electrochimica Acta, 2013, in press, doi: ttp://dx.doi.org/10.1016/j.electacta.2013.07.150
Communications in Conferences
1.
A.M. Oliveira Brett, A.–M. Chiorcea, I.R. Fernandes, T.R.A. Macedo, M. Vivian, S.H.P.
Serrano, A DNA–electrochemical biosensor for monitoring DNA–drug interactions,
50th International Society of Electrochemistry Meeting, Pavia, Italy, 5 – 10 September
1999.
2.
A.M. Oliveira Brett, A.–M. Chiorcea, Imaging guanosine by scanning probe
microscopy, 5th Workshop “Biosensors for Direct Monitoring of Environmental
Pollutants in the Field, Otrokovice, Check Republic, 8 – 10 October 1999.
3.
A.M. Oliveira Brett, A.–M. Chiorcea, Scanning probe microscopy of guanosine on
gold electrode, In Situ Nano Biochemistry – 2000 Workshop, The Royal Danish
Academy of Sciences and Letters, Copenhagen, Denmark, 15 – 18 March 2000.
4.
A.M. Oliveira Brett, J.A.P. Piedade, A.–M. Chiorcea, Scanning probe microscopy of
adriamycin on highly oriented pyrolytic graphite, 10º Encontro da Sociedade
Portuguesa de Materiais (SPM), Materiais’ 2001, 1st International Materials Symposium,
Coimbra, Portugal, 9 – 11 April 2001.
5.
A.–M. Chiorcea, A.M. Oliveira Brett, Scanning probe microscopic imaging of guanine
on a highly oriented pyrolytic graphite electrode, XVIth International Symposium on
Bioelectrochemistry and Bioenergetics, Bratislava, Slovak Republic, 1 – 6 June 2001.
6.
A.M. Oliveira Brett, J.A.P. Piedade, A.–M. Chiorcea, Biossensor electroquímico de
DNA, 5as Jornadas do Instituto de Ciência e Engenharia de Materiais e Superfícies
(ICEMS), Coimbra, Portugal, 26 – 27 October 2001.
7.
A.–M. Chiorcea, A.M. Oliveira Brett, An electrochemical atomic force microscopy
study of DNA adsorbed on a highly oriented pyrolytic graphite electrode surface,
2nd Workshop of the Concerted Action. “Evaluation / Validation of Novel Biosensors in
Real Environmental and Food Samples”, Florence, Italy, 18 – 20 April 2002.
8.
A.M. Oliveira Brett, A.–M. Chiorcea, V.C. Diculescu, Biossensor electroquímico com
DNA, XV Congresso da Sociedade Ibero–Americana de Electroquímica, Évora, Portugal,
8 – 13 September 2002.
9.
A.M. Oliveira Brett, A.–M. Chiorcea, J.A. Piedade, V. Diculescu, I. Rebelo, S.H.P.
Serrano, M.A. La–Scalea, Analysing structure–function relationship with
electrochemical DNA–biosensors, 53rd Annual Meeting of the International Society of
Electrochemistry, Düsseldorf, Germany, 15 – 20 September 2002.
10. A.–M. Chiorcea, A.M. Oliveira Brett, Electrochemical atomic force microscopy of
adriamycin adsorbed on a highly oriented pyrolytic graphite electrode surface, 53rd
Annual Meeting of the International Society of Electrochemistry, Düsseldorf, Germany,
15 – 20 September 2002.
9
11. A.–M. Chiorcea, A.M. Oliveira Brett, AFM of guanine adsorbed on HOPG under
electrochemical control, NATO Advanced Study Institute, Scanning Probe Microscopy:
Characterization, Nanofabrication and Device Application of Functional Materials,
Albufeira, Portugal, 1 – 13 October 2002.
12. A.M. Oliveira Brett, A.–M. Chiorcea, Atomic force microscopy characterisation of an
electrochemical
DNA–biosensor,
XVIIth
International
Symposium
on
Bioelectrochemistry and Bioenergetics, Florence, Italy, 19 – 24 June 2003.
13. A.–M. Chiorcea, A.M. Oliveira Brett, Effect of pH and applied potential on the
adsorption of DNA on highly oriented pyrolytic graphite electrodes, XVIIth
International Symposium on Bioelectrochemistry and Bioenergetics, Florence, Italy, 19 –
24 June 2003.
14. A.M. Oliveira Brett, A.–M. Chiorcea, J.A. Piedade, V. Diculescu, I. Rebelo,
Electrochemical DNA–biosensors. Surface AFM characterization and applications,
54th Annual Meeting of the International Society of Electrochemistry, São Pedro, Brasil
31 August – 5 September 2003.
15. A.M. Oliveira Brett, A.–M. Chiorcea, V. Diculescu, Applications of an electrochemical
DNA biosensor, International Workshop on “Biosensors for Food Safety and
Environmental Monitoring”, Marrakech, Morocco, 9 – 11 October 2003.
16. A.M. Oliveira Brett, A.–M. Chiorcea V. Diculescu, T.S. Oretskaya, Synthetic
oligonucleotides: AFM characterization and electroanalytical studies, Workshop on
“New Trends in Nucleic Acids Based Biosensors”, Florence, Italy, 5 – 28 October 2003.
17. A.M. Oliveira Brett, A.–M. Chiorcea, Atomic force microscopy of DNA immobilized
onto a highly oriented pyrolytic graphite electrode surface, SMCBS International
Workshop on Surface Modification for Chemical and Biochemical Sensing, Bialowieza,
Poland, 13 – 16 November 2003.
18. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Atomic force microscopy
characterization of synthetic oligonucleotides adsorbed onto highly oriented
pyrolytic graphite, Life Sciences Interface Spring School, Nottingham, UK, 29 March –
2 April 2004.
19. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Atomic force microscopy
characterization of synthetic oligonucleotides adsorbed onto highly oriented
pyrolytic graphite, UK SPM 2004, A Royal Microscopical Society Meeting,
Nottingham, UK, 30 – 31 April 2004.
20. A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, V. Diculescu, T.S. Oretskaya, AFM
characterisation and electroanalytical studies of synthetic oligonucleotides, The
Eighth World Congress on Biosensors, Granada, Spain, 24 – 26 May 2004.
21. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Imaging synthetic oligonucleotides and
homopolymer nucleic acids by atomic force microscopy, EMBO/FEBS Workshop on
AFM Applications in Biology, Instituto Gulbenkian de Ciência, Oeiras, Portugal, 7 – 9
July 2004.
10
22. A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, AFM characterization and applications
of electrochemical DNA–biosensors, International Conference on Electrified Interfaces,
10th in a Series on Non–Traditional Methods, SPA, Belgium, 11 – 16 July 2004.
23. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Effect of sequence and applied potential
on the adsorption of nucleic acids molecules on HOPG electrodes. AFM surface
characterization, International Society of Electrochemistry, 55th Annual Meeting,
Thessaloniki, Greece, 11 – 24 September 2004.
24. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, AFM characterization of synthetic
nucleotides and homopolymer nucleic acids adsorbed onto HOPG, International
Society of Electrochemistry, 55th Annual Meeting, Thessaloniki, Greece, 11 – 24
September 2004.
25. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, AFM characterization of DNA
nanoscale biosensor films prepared under applied potential, XVIII International
Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society
(BES) and 3rd Spring Meeting of the International Society of Electrochemistry (ISE),
Coimbra, Portugal, 19 – 24 June 2005.
26. A.–M. Chiorcea–Paquim, T.S. Oretskaya, A.M. Oliveira Brett, Atomic force
microscopy characterization of synthetic poly– and oligo–homonucleotides adsorbed
onto HOPG, XVIII International Symposium on Bioelectrochemistry and Bioenergetics
of the Bioelectrochemical Society (BES) and 3rd Spring Meeting of the International
Society of Electrochemistry (ISE), Coimbra, Portugal, 19 – 24 June 2005.
27. V.C. Diculescu, A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Electrochemical
analysis of natural and synthetic polynucleotides, XVIII International Symposium on
Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society (BES) and 3rd
Spring Meeting of the International Society of Electrochemistry (ISE), Coimbra,
Portugal, 19 – 24 June 2005.
28. A.–M. Chiorcea–Paquim, J.A.P. Piedade, R. Wombacher, A. Jäschke, A.M. Oliveira
Brett, Atomic force microscopy and anodic voltammetry characterization of 49–mer
Diels–Alderase ribozymes, XVIII International Symposium on Bioelectrochemistry and
Bioenergetics of the Bioelectrochemical Society (BES) and 3rd Spring Meeting of the
International Society of Electrochemistry (ISE), Coimbra, Portugal, 19 – 24 June 2005.
29. A.M. Oliveira Brett, V.C. Diculescu, J.A.P. Piedade, A.–M. Chiorcea–Paquim, DNA–
electrochemical nanoscale biosensor films on carbon electrode surfaces: AFM
characterization and applications, 40th IUPAC Congress, Beijing, China, 14 – 19
August 2005.
30. C.M.A. Brett V.A. Alves, A.–M. Chiorcea–Paquim, A. Cavaleiro, Influence of steel
nanostructure on electrochemical corrosion resistance, The Europeen Corrosion
Congress, Lisbon, Portugal, 4 – 7 September 2005.
31. A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, AFM characterization of DNA–
electrochemical nanoscale biosensor films on carbon electrode surfaces, The 56th
Annual Meeting of the International Society of Electrochemistry, Bussan, Korea, 25 – 30
September 2005.
11
32. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Electroanalysis of natural and synthetic
polynucleotides, SPQ–Analítica’05, 5º Encontro da Divisão de Química Analítica da
SPQ, Coimbra, Portugal, 27 – 28 October 2005.
33. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Effect of applied potential on the
adsorption of synthetic pyrimidinic oligonucleotides onto HOPG electrode, Second
International Workshop on Biosensors for Food Safety and Environmental Monitoring,
Agadir, Morocco, 10 – 12 November 2005.
34. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, Atomic microscopy characterization of
nucleic acids nanofilms adsorbed onto HOPG electrode surfaces, 57th Annual
Meeting of the International Society of Electrochemistry, Edinburgh, UK, 27 August – 1
September 2006.
35. A.M. Oliveira Brett, A.–M. Chiorcea–Paquim, J.A.P. Piedade, V.C. Diculescu, DNA–
electrochemical nanoscale biosensor films: AFM characterization and applications,
Química Sustentável, Um Tema. Uma Reflexão, XX Encontro Nacional Sociedade
Portuguesa de Química, Campus de Caparica – FCTIUNL, Portugal, 14 – 16 December
2006.
36. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, AFM characterization of synthetic
homo and hetero–oligonucleotides adsorbed onto HOPG, Linz Winter Workshop,
Linz, Austria, 2 – 5 February 2007.
37. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, AFM characterization of nucleic acid
nanofilms on charged surfaces, XIXth International Symposium on
Bioelectrochemistry and Bioenergetics, Toulouse, France, 1 – 4 April 2007.
38. A.–M. Chiorcea–Paquim, A.M. Oliveira Brett, AFM characterization of DNA–
biosensor nanofilms adsorbed onto charged surfaces, AFM BioMed Conference –
International Meeting on AFM in Life Sciences and Medicine, Barcelona, Spain, 19 – 21
April 2007.
39. A.M. Oliveira Brett, M. Vivian, A.–M. Chiorcea–Paquim, V.C. Diculescu, In situ
sensing of DNA damage using a nanoscale film DNA–electrochemical biosensor,
First Iberic Meeting on Medical Chemistry Anticancer Agents, Régua, Douro, Portugal,
28 April – 1 May 2007.
40. V.C. Diculescu, A.–M. Chiorcea–Paquim, O. Corduneanu, M. Garnett, A.M. Oliveira
Brett, Electrochemistry for probing in situ DNA damage, 58th Annual Meeting of the
International Society of Electrochemistry, Banff, Canada, 9 – 14 September 2007.
41. V.C. Diculescu, A.–M. Chiorcea–Paquim, O. Corduneanu, P. Janeiro, S.C.B. Oliveira,
T.A. Enache, A.M. Oliveira Brett, Nanobioelectroquímica: Caracterização de
eléctrodos modificados e aplicações, XVIII Congreso de la Sociedad Iberoamericana de
Electroquímica, Medellin, Colombia, 10 – 14 March 2008.
42. A.M. Oliveira Brett, S.C.B. Oliveira, O. Corduneanu, V.C. Diculescu, A.–M. Chiorcea–
Paquim, In situ evaluation of hazard compounds–DNA interactions using na
electrochemical DNA–biosensor, 6th Spring Meeting of the International Society of
Electrochemistry, Foz do Iguaçu, Brazil 16 – 19 March 2008.
12
43. A.–M. Chiorcea–Paquim, O. Corduneanu, V.C. Diculescu, A.M. Oliveira Brett, Atomic
force microscopy and electrochemical studies of palladium nanoparticles and
nanowires electrodeposited onto carbon electrode surfaces, NanoSpain2008, Braga,
Portugal, 14 – 18 April 2008.
44. V.C. Diculescu, A.–M. Chiorcea–Paquim, O. Corduneanu, A.M. Oliveira Brett,
Electrochemical behaviour of palladium nanoparticles deposited onto carbon
surfaces, 1st Regional Sumposium on Electrochemistry on South – East Europe, Rovinj,
Croatia, 4 – 8 May 2008.
45. O. Corduneanu, A.–M. Chiorcea–Paquim, V. Diculescu, A.M. Oliveira Brett,
Characterization of Electrodeposited Palladium Nanoparticles and Nanowires on
Carbon Electrodes, ESEAC–2008. 12th International Conference on Electroanalysis,
Prague, Czech Republic, 16 – 19 June 2008. Best poster Award, 3rd place.
46. A.–M. Chiorcea–Paquim, O. Corduneanu, V. Diculescu, A.M. Oliveira Brett, Atomic
force microscopy and voltammetric characterization of lipoic acid – palladium
complexes adsorbed on carbon electrode surfaces, Seeing at the Nanoscale VI –
Exploring the Future of Nanotechnology Using SPM and Related Techniques, Berlin,
Germany, 9 – 11 July 2008.
47. O. Corduneanu, A.–M. Chiorcea–Paquim, Merrill Garnett, A.M. Oliveira Brett, Lipoic
acid – palladium complexes adsorbed on carbon electrode surfaces. Voltammetric
and AFM study, The 59th Annual Meeting of the International Society of
Electrochemistry, Seville, Spain, 7 – 12 September 2008.
48. A.–M. Chiorcea–Paquim, O. Corduneanu, V. Diculescu, A.M. Oliveira Brett, Palladium
nanoparticles and nanowires electrodeposited onto carbon electrodes: AFM and
electrochemical studies, The 59th Annual Meeting of the International Society of
Electrochemistry, Seville, Spain, 7 – 12 September 2008.
49. A. – M. Chiorcea – Paquim, T.S. Oretskaya, A.M. Oliveira – Brett, Effect of pH on the
adsorption of synthetic oligonucleotides onto highly oriented pyrolytic graphite
electrode, International Spring School of Biophysics & Bioelectrochemistry for
Medicine, Cisnadioara, Romania, 6 – 10 May 2009.
50. V.C. Diculescu, A.–M. Chiorcea–Paquim, L. Tugulea, M. Vivian, Ana Maria Oliveira
Brett, Voltammetric study of in situ DNA oxidative damage and interaction with
liposomes of the antileukemia drug imatinib, XXth Symposium of the International
Society of Bioelectrochemistry (BES 2009), Sibiu, Romania, 10 – 14 May de 2009.
51. A.–M. Chiorcea–Paquim, O. Corduneanu, V.C. Diculescu, Ana Maria Oliveira Brett,
Interaction of DNA with palladium and palladium containing compounds. Atomic
force microscopy characterization, XXth Symposium of the International Society of
Bioelectrochemistry (BES 2009), Sibiu, Romania, 10 – 14 May de 2009.
52. A. – M. Chiorcea – Paquim, A.M. Oliveira Brett, Electrochemical atomic force
microscopy characterisation of guanosine and guanine self – assemblies onto a
HOPG electrode surface, ESF Research Conference on Self – Assembly of Guanosine
Derivatives: From Biological Systems to Nanotechnological Applications, Obergurgl,
Austria, 20 – 25 June 2009.
13
53. A. – M. Chiorcea – Paquim, V.C. Diculescu, L. Tugulea, A.M. Oliveira – Brett, Atomic
force microscopy characterization of imatinib – liposomes interactions, Journées
d’Electrochimie, Sinaia, Romania, 6 – 10 July 2009.
54. A.M. Oliveira – Brett, S.C. Oliveira, O. Corduneanu, V.C. Diculescu, A. – M. Chiorcea –
Paquim, In situ sensing of DNA damage with a nanoscale DNA – electrochemical
biosensor, 12th International Symposium on Electroanalytical Chemistry (12th ISEC),
Changchun, China, 13 – 15 August 2009.
55. A.M. Oliveira Brett, S.C.B. Oliveira, O. Corduneanu, V.C. Diculescu, A. – M. Chiorcea
– Paquim, DNA – Nanoscale Electrochemical Biosensor: AFM Characterization and
Applications, 60th Annual Meeting of the International Society of Electrochemistry
(ISE), Peking, China, 16 – 21 August 2009.
56. O. Corduneanu, V.C. Diculescu, A. – M. Chiorcea – Paquim, A.M. Oliveira – Brett,
Electrodeposition of palladium nanoparticles and nanowires onto carbon substrates,
International Symposium on Electrochemistry for Energy Conversion and Storage
(ISEECS), Wuhan – Three Gorges, China, 22 – 26 August 2009.
57. V.C. Diculescu, A. – M. Chiorcea – Paquim, P. Santos, A.M. Oliveira – Brett,
Electrochemistry and AFM studies of DNA and Guanine – rich oligomers structural
modifications, Training School on Fundamentals of guanosine self – assembly and
quadruplex formation, Ischia, Italy, 27 – 29 October 2009.
58. V.C. Diculescu, A.D.R. Pontinha, S.M.A. Jorge, M. Vivan, A.M. Chiorcea Paquim, A.M.
Oliveira Brett, In situ electrochemical evaluation of methotrexate-DNA damage, 13th
International Conference on Electroanalysis, ESEAC, 20 – 24 June 2010, Gijon, Spain.
59. V.C. Diculescu, A.M. Chiorcea Paquim, M. Vivan, L. Tugulea, A.M. Oliveira Brett,
Estudo voltamétrico da interacção com DNA e incorporação em liposomas de
derivados fenilaminopirimidinicos com propriedades anticancerígenas, Congreso da
Sociedad Iberoamericana de Electroquímica (SIBAE), Alcalá de Henares, Spain, 27 June
– 2 July 2010.
60. I.C. Lopes, P.V.F. Santos, V.C. Diculescu, M.C.U. de Araújo, A.A. Tanaka, A.M.
Oliveira Brett, Oxidação electroquímica da microcistina-LR, Congreso da Sociedad
Iberoamericana de Electroquímica (SIBAE), Alcalá de Henares, Spain, 27 June – 02 July
2010.
61. A.M. Oliveira Brett, V.C. Diculescu, A.M. Chiorcea Paquim, R. Eritja, Guanosinequadruplex Structures in Thrombine Binding Aptamer - AFM and Voltammetric
Characterization, The 61st Annual Meeting of the International Society of
Electrochemistry - ISE 2010, 26 September – 1 October 2010.
62. A.D. Pontinha, V.C. Diculescu, S.M.A. Jorge, M. Vivan, A.M. Chiorcea Paquim, A.M.
Oliveira Brett, Voltammetric and AFM Study of Methotrexate-DNA Interaction, The
61st Annual Meeting of the International Society of Electrochemistry - ISE 2010, Nice,
France, 26 September – 1 October 2010.
63. V.C. Diculescu, A.M. Chiorcea Paquim, O. Corduneanu, S. Fiuza, M.P. Marques, A.M.
Oliveira Brett, DNA Interaction with Palladium Chelates of Biogenic Polyamines –
AFM and Voltammetric Characterization, The 61st Annual Meeting of the
14
International Society of Electrochemistry - ISE 2010, Nice, France, 26 September - 1
October 2010.
64. P.V.F. Santos, A.M. Chiorcea Paquim, A.M. Oliveira Brett, Quadruplex formation of
guanine containing oligodeoxynucleotides: voltammetric and AFM characterization,
Third International Meeting on G-quadruplexes and G-assembly, Sorrento (Italy), June
28 – July 1, 2011.
65. A.M. Chiorcea Paquim, P. Santos and A.M. Oliveira Brett, Effect of pH and secondary
structure on the adsorption and voltammetric behaviour of guanine-containing
Oligodeoxynucleotides, Third Regional Symposium on Electrochemistry South-East
Europe (RSE–SEE3), Bucharest, Romania, May 13 – 17, 2012.
66. A.M. Chiorcea Paquim, P. Santos, R. Eritja and A.M. Oliveira Brett, Atomic force
microscopy and voltammetric characterisation of quadruplex formation in guaninecontaining oligodeoxynucleotides, 63rd Annual Meeting of the International Society of
Electrochemistry, Prague, Czech Republic, 19 – 24 August, 2012.
67. V.C. Diculescu, A.M. Chiorcea Paquim, A.M. Oliveira Brett, DNA-electrochemical
biosensors for the detection of specific interactions and oxidative damage to DNA,
IV Simposio Chileno de Electroquímica da sociedade Chilena de Química, División de
Electroquímica, Olmué, V Región Valparaíso, Chile, November 28 – 30, 2012.
68. A.M. Oliveira Brett, P.V. Santos, A.M. Chiorcea Paquim and V.C. Diculescu,
Self-assembled
G-quadruplex
nanowires:
AFM
and
electrochemical
characterisation, Fifth ISEAC Triennial International Conference on Advances and
Recent Trends in Electrochemistry, Ramoji Film City, Hyderabad, India, January 16 – 20,
2013.
69. A.M. Chiorcea Paquim, P.V. Santos, A.M. Oliveira Brett, Atomic Force Microscopy
and Voltammetric Characterization of Homo-Oligodeoxynucleotides, Biochemistry
2013, 12th Topical Meeting of the International Society of Electrochemistry and XXII
International Symposium on Bioelectrochemistry and Bioenergetics of the
Bioelectrochemical Society, Bochum, Germany, 17 – 21 March, 2013.
70. A.M. Oliveira Brett, A.M. Chiorcea Paquim, V.C. Diculescu, A.D.R. Pontinha, S.C.B.
Oliveira, In situ sensing of DNA oxidative damage with DNA-nanostructured
electrochemical biosensors, -FASC, The Second International Symposium on
“Analytical Chemistry for Sustainable Development” – ACSD 2013 – The 4th Federation
of African Societies of Chemistry (FASC) Congress, Marrakech, May 7 – 9, 2013.
71. A.M. Oliveira Brett, V.C. Diculescu, A.M. Chiorcea Paquim, A.D.R. Pontinha, S.C.B.
Oliveira, DNA-electrochemical biosensors for investigating DNA damage based on
structural changes, Summer School on Electrochemistry for environmental and medical
applications, Cluj-Napoca, Romania, June 17 – 21, 2013.
72. A.M. Oliveira Brett, A.M. Chiorcea Paquim, R. Eritja, A.D.R. Pontinha, Effect Of Na+
and K+ ions on G-Quadruplex nanostructures structural conformations, 44th World
Chemistry congress of IUPAC, Istanbul, Turkey, August 11 – 16, 2013.