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SIJMMARY SHEET OF
MOSQ'IJITO LARVAL TRAP
TESTING CONDUCTED AT
THE UNIVERSITY OF FLORIDA
Background: Experiments were conducted during the months of August, September,
and November, 2003, utilizing the original patented mosquito larva traps (U.S. Patent No.
6,886,293). Subsequent to these and other tests, revisions were made that further
enhanced the trapping abilities of the product (U.S. Patent No. 7,134,238).
Objectives: After determining the proper test procedures, the objectives of the testing
were to:
1) Ascertain trap effectiveness.
2) Identify potential stimulants to further attrac,t gravid mosquitoes.
Methodology: Each test consisted of the deployment of twenty traps in various locations
around the Entomology building on campus. Although this deployment in a small area
diminished the attractant abilities of any single trap due to competition Aom nearby units,
the overall results of the tests in relation to total kills and best stimulants verses the
control units containing water only remain valid.
The dedinitive test results are found in the second round of testing; the first round being
utilized to refine the methods used in the second round. The results of this second testing
are portrayed in graph form on page 15 of the attached report. The combined capture rate
of the traps over a thirty-eight day period was estimated to be 17,400 mosquitoes or
approximately 458 mosquitoes per day.
(1 16 egg rafts (page 15) x 150 eggs per raft (page 3) = 17,400 mosquitoes)
Summary: Gravid (egg bearing) mosquitoes generally live their entire lives,
(approximately 15 days as adults), in an area about the size of a football field (less than '/z
acre) and lay eggs 3 - 5 times during their adult Iife. The Bite Back! traps collect and
destroy the offspring of the adults, thus breaking the exponential cycle of mosquito
infestation in a given area during the breeding months.
Field deployment of traps has proven the product to be highly effective in mosquito
eradication while being safe to use around children and pets. With the stimulant being
rice based, the Bite Back! trap uses no poisons or harmful chemicals. Rather than
electrocution or death by poison, the Bite Back! trapping action relies on the natural
tendencies of the mosquito larva to dive for protection and rest.
Conclusion: This product is the only mosquito control system available today that is
environmentally friendly, truly safe to use around children and pets, has been tested and
proven effective in eliminating mosquito problems, and is inexpensive to deploy and
maintain.
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f.&$3:$/& UNIVERSITY OF
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FLORIDA
Institute of Food and Agricultural Sciences
Natural Area Drive
PO Box 110620
Gainesville, F1 3261 1-0620
(352) 392-1901
Fax (352) 3924190
Entomology & Nernatology Department
December 2,2003
James D. Forehand
42 Parkstone Court
Stone Mountain, Georgia 30087
Dear Jimmy
The Forehand Mosquito Larval Trap report is enclosed.
/;I"
J. F. But r, Professor Med. Vet. Entomology
SUBJECT:
Test Forehand Mosquito Larval Traps.
PEST:
Mosquitoes.
TITLE:
Forehand Mosquito Larval Trap Evaluations Trial 1 and 2.
LEADER: Dr. J. F. Butler,
Department of Entomology and Nematology, IFAS, University of Florida,
Bld 970, Natural Area Drive
Box 1 10620
Gainesville, Florida 326 11-0620.
(352 392-1930-152)
E-mail= jfl>@trnv.ifas.ufl .edu
Dr. J. Maruniak
Department of Entomology and Nematology, IFAS, University of Florida,
Bld. 970, Natural Area Drive
Box 110620
Gainesville, Florida 326 11-0620
COOPERATORS:
James D. Forehand
42 Parkstone Court
Stone Mountain, Georgia 30087
James Forehand" jdf~rehand~earthiin
k .net
OBJECTIVES:
The objectives were: (1.) To develop testing procedures to evaluate
mosquito traps. (2.) To determine the ef-fectiveness of the Forehand inverted cone
mosquito larval trap. (3.) To add potential egg laying stimulants to the trap to
improve egg and larval capture rate.
MATERIALS and METHODS:
Mosquito Larval Trap Trial 1: Forehand Mosquito LarvalJEgg Traps were
evaluated with different types of media as an attractant for natural egg laying.
Five treatments with four replications were used (20 traps). Traps were placed in
four different areas near the Entomology and Nematology Building 970. Trap
treatments were randomized with 4 replications per treatment. Traps were labeled
with date, treatment number, and location with white tape placed on the trap side.
A one by six cm strip of filter paper was placed in the cone for egg attachment.
Medias evaluated in Trap 1 are as follows:
Media 1:
2 Liters of water
2 grams of powdered Pot Bellied Pig Chow
0.5 gram of brewers yeast
Media 2:
2 Liters of water
2 grams of powered Timothy Hay
Media 3:
2 Liters of water
2 grams of powered Pure Rice Bran
Media 4:
2 Liters of water
2 grams of powered Alfalfa Pellets
Media 5:
2 Liters of water
The traps with media were placed outside but protected from rain by a roof
overhang. The windowsills of the inner courtyard and out side of building 970
were used. Observations were made after the first 7 days to determine if
mosquitoes had begun laying eggs. The number of egg rafts present on the water
surface as well as individual eggs placed on small blotter paper strips at the side
of the cone leading into the water was monitored. After 5 weeks the residue in
the traps was evaluated for mosquito larvae and other arthropods present. Adult
mosquitoes in the upper trap area were monitored on a weekly basis.
Mosquito Larval Trap Trial 2:
Forehand Mosquito Larval Traps were set up as in Trial 1. Five treatments
with four replications were used. The traps with media were placed outside of the
windowsills in four different areas of building 970 in the inner courtyard.
Evaluation of egg rafts and egg deposition were the only data monitored for Trial
2 as these criteria were found to be the most reliable data from Trial 1. The one by
six cm strip of filter paper was placed in the cone for egg attachment and
attractant placement. The strip was changed every 4 days. The following media
were evaluated in Trial 2.
Media 1:
1 gram of powered Pure Rice Bran
1 gram of powered Alfalfa Pellets
2 Liters of water
Media 2:
2 Liters of water
2 grams of powered Pure Rice Bran
0.5 gram of brewers yeast in
Media 3:
2 Liters of water
Media 4:
1 gram of powered Pure Rice Bran
0.005 gram of attractant on the top of the attached iilter paper weekly
2 Liters of water
Media 5:
2 Liters of water
Observations were made as noted in the tables for the number of mosquito
egg rafts present on the surface of the water and the individual eggs attached to
the filter paper inserts. Previous counts of mosquito larvae and other insects
present in Trial 1 were limited in value as the number of mosquitoes that died in
the I" and 2nd instar stages could not be evaluated. The most valuable counts in
Trial 1 were the number of egg rafts in the surface of the water and the number of
individual eggs present on the attached paper strip. These gave a better evaluation
of the potential mosquitoes exposed in the trap. A mosquitoe normally produces
2-3 egg rafts in her lifetime due to natural mortality rates. Therefore one egg raft
or egg deposition represents about one third of their lifetime expected
reproductive potential. A mosquito egg raft contains about 150 eggs. The
observations of egg raft counts were timed to record the numbers prior to hatching
and dispersal of 1"' instar larvae into the trap.
Observations as egg and egg rafts were made daily for 2 weeks to determine
the number of mosquito eggs laid. Adult mosquitoes surviving in the upper trap
area were monitored on a weekly basis.
RESULTS and DISCISSION:
There were three objectives: (1.) To develop testing procedures to evaluate
mosquito traps. (2.) To determine the effectiveness of the Forehand inverted cone
mosquito larval trap. (3.) To evaluate an egg laying stimulants added to the trap.
Trial 1 was a developmental step to determine how data could be collected
for this new type of trap and to measure potential collection rates. As mosquitoes
visiting and laying eggs in the trap could not be counted the egg rafts or
individual eggs were counted within a time interval and summed over time for
evaluation. As the media matured with fermentation the mosquito egg laying
attraction generally increased (Figure 5). When the eggs hatched the small larvae
generally migrated beneath the black funnel to the lighter area beneath and were
trapped and drown A few first instar larvae were able to migrate through the
small holes in the retention ring where they continued development to adults.
As the media became older or about 2 weeks there was a debris buildup
allowing some of the larvae to stay in the black funnel. We were not able to assess
the numbers of larvae that were trapped and drown. The total number of
mosquitoes present was estimated by the number of egg rafts, which were on the
surface. An egg raft would result in 150 larvae for Clulex quuinyuqfu,~c~utus.
Aedes
ulhopictus females lay eggs singularly at or above the water line. These were
assessed by placing 1 cm strips of blotter paper from the top of the trap into the
water at the center. Success considered for A e h sp. was limited, as the number
of eggs collected seemed low. I think that the surface of the shiny black funnel
should be rough or non-glossy allowing for some moistening of the plasticlwater
interface.
Data collected for Trial 1 are presented in Tables 1-5. Results of Trial 1 are
best shown in Figures 1-3. There were no significant differences in Trial 1 (Table
4,5) due to the placement of the replications on the outer windowsills of building
970. The mosquito populations on the outside of the building were not
comparable with the inner courtyard. We were able to develop assay methods for
the trapping system however, with egg rafts on the surface of the water and eggs
placed upon the filter paper used to determine the number of potential mosquitoes
developing. The presence and number of trapped insects were varied (Table 1). It
was difficult to evaluate survivingldead insect numbers from those remaining and
decaying in the trap so the number of eggs and egg rafts were adopted as a
measure of potential mosquitoes exposed. Some adult mortality was seen in those
insects trapped above the retention ring of the trap.
The suitability of media in the traps was also observed. Table 1 gives the
general observations on trap conditions and catches rates for traps during August
2003. In Trial 1 the condition and odor produced by the media was noted with the
powered Pot Bellied Pig Chow and brewers yeast being particularly rank. This
media would not be acceptable to the general public. This media is also not on
the GRAS list. The other media were more acceptable and the ingredients are on
the list.
The media collecting the most C'ule,r quinqu~f~~sciutus
was the Powered
Pure Rice Bran and brewers yeast (Figure 2). This material could easily be
packaged with the traps. Aedes eggs were highest for Powered Timothy hay and
brewers yeast Figure I ). Powered Timothy hay is not on the GRAS list. The
fermenting pig chow also attracted many other small Diptera maggots as
demonstrated in Figure 3 and Table 1. Statistical dif'ferences could not be
demonstrated in Trial 1 due to high variance in the replications presented between
to outer windowsill and inner courtyard placement of traps.
Trial 2 was more statistically reliable with replication placement allowing
data collected to be analyzed. It also gave a measure of suitable media and
augmentation of trap catch rates with potential attractants. Table 6-8 and Figures
4-6 presents these results. In this Trial powdered Rice Hulls and brewers yeast
media were compared with powered Alfalfa Pellets and brewers yeast as well as
their mixtures (Figure 5). The powdered Rice Hulls was significantly more
attractive than Rice + Alfalfa + Dibutyl succinate which was significantly better
than Alfalfa alone (Tables 6-8). The mixture of powdered Rice Hulls + powered
Alfalfa Pellets were not significantly different than the water control. The
mixtures of Rice Hulls + powered Alfalfa Pellets and Rice Hulls + powered
Alfalfa Pellets + Dibutyl Succinate cannot be directly compared to the other
treatments as the amount of each in the mixture was less than the other treatments.
This was to maintain the same total 2 g amount of dry media at one gram of each
in the mixture tested. The significant difference seen between the Rice Hulls +
Alfalfa Pellets and Rice Hulls + Alfalfa Pellets + Dibutyl Succinate demonstrated
that Dibutyl Succinate increased the catch rates for the traps. When Dibutyl
Succinate was added to the filter paper on the top of the trap (0.005 g) the number
of C'ulex yuinyu~firsc~utu.s
egg rafts were doubled indicating that this material is a
good attractant for stimulation of egg laying (Figure 5).
Media conditioning and longevity is noted in Figure 5. A delay of 12 days
was noted before mosquitoes were attracted to the traps. By day 29 the media had
lost its major attractiveness. Traps will need to be serviced about once per month,
cleaning out the debris and adding new water and media.
SUMMARY:
The powdered rice hulls at 2 grams + 0.5 g brewers yeast, in 2 Liters of
water was the best both in numbers of eggs laid and suitability of product odor.
One loading of a trap is good for about 30 days and then productivity goes to near
0. Our best data collection was a count of mosquito egg rafts (150 eggsiraft), as
larval mortality could not be reliably estimated. We had some survival of
mosquito larvae to adults during the test. The traps may need to be modified and
or disturbed to assure trapping of all the larvae. I think that 6 small 2 cm no return
cones with 3mm holes placed in the positioning ring (the ring just under the black
funnel) would allow larvae to move into this area to breath air but be unable to
return to the center exit of the trap. Larva in this region of the trap would survive
to adult and then die allowing a precise trapping measure of numbers and species.
This would also be a visible demonstration of adult mosquitoes being trapped and
killed for the consumer. The water levels in the traps will also need to be
maintained to keep traps effective.
The trap that included Dibutyl Succinate as an attractant demonstrated
double the catch rate of one gram each of Rice Hulls and Alfalfa. I assume that it
would also work for rice hulls alone. The mosquitoes, which are the vectors of
West Nile Virus (Culex yuinquqfiscicrtus), were the majority those trapped.
Aedes mosquitoes were not attracted to laying eggs on the slick black surface and
only laid a few eggs on the filter paper installed for the purpose. The shiny black
surface should be converted to a mat or brushed surface to one centimeter above
the water line, which may increase the egg laying for these mosquitoes.
Table 1: Forehand Mosquito Larval cjeneral observations of Insects
Collected and Media Conditions in T r a ~ s .
a - alfalfa
p - Pot Bellied Pig Chow
r - powered Pure Rice Bran
t - Timothy hay
W- water (control)
la (translucent green, neutral smell) I egg raft
9- 10 each 2-3rd instars larvae (dead)
1 each 4th instar mosquito Larva (dead)
I p (near putrid)
1 egg raft
1 adult Culex yuinyuef~~rc~atus
adult female
1 Pupa
25 dead adult C'x y uiny ztejusciatus.
4 each 4th instar C'x. yurnquefa.scratus larvae
2 each 3rd instar larvae
Estimated 65 2nd instar larvae
1r (putrid, brown milky color)
40 each 2-3rd instar mosq larvae
1 sewer fly, Psychodidae
1 fruit fly, Drosophilidae
2 adult 0.yuinquefasciutus females, 1 male
1t (not putrid, translucent brown)
1 egg raft
9 Chironomid larvae
1 Psychodidae adult (dead)
1w (clear water)
3 Culicid larvae
4 Chironomidae larvae
2 each 4th instar (:x. y uinquefu.~cratus
2a (brownish-green, slight musky smell)
80 each 2-3rd instar mosquito larvae
2p (brown putrid)
1 pupal cast skin mosq
84 each Chironomid cases on clear plastic part of trap
9 each Chironomid cases on black lip
2 adult female Ck. yuinyuefusciutus, I male
2r (brown putrid)
1 egg raft
2 each 4th instar mosquito larvae
10 each 2-3rd instar mosquito larvae
2 Chironomid larvae (bloodworms)
2t (brown, slight non-putrid odor)
Collembola; about 10 each dead on water
1 Psychodidae adult dead
1 Psychodidae larva
2w
nothing
Site 1 - inner courtyard, west
Site 2 - inner courtyard, east
Site 3 - outer courtyard, west
Site 4 - outer courtyard, east
3a (heavily green, lots of algae)
1 each 1st instar mosquito larva
1 adult female Clx. qurnyuefr.~ciatus
3p (green water, nasty putrid)
I each 2nd instar mosquito larva (dead)
3r (green water, slight odor)
98 each 2-4th instar mosquito larvae (dead)
3t (green, slight odor)
nothing
3w (some algae, very slight)
nothing
4a (brown, moderate stink)
1 each adult Culex (escaped)
3 each 4th instar larvae
15 pupae
75 each 4th instar C'x. quinyucfr.vcrutus
4p (putrid brown)
1 mosquito pupa
Approximately 100 Chironomid larval cases
4r (putrid brown, orange fjlm on bottom of trap)
26 each 3-4th instar mosquito larvae Cx. quingu~f~~~crutus.
Several dead Collembola
4t (brown, some odor)
105 each mosquito larvae,
2-4th instar C'x. yuinqu~fuscnrtus
50 immature cyclorraphids
15 mosq larvae, 2-4th instar, C'x. Qurnyu~fuscrutus
4w
Table 2: Forehand Mosquito Larval Traps Trial 1
Mosauito Larvae Present in Traps
Media tested
S1
S2
S3
S4
11
80
2
Alfalfa
Pig
98
1
3
Rice
42
14
98
Timothy
0
0
0
Water
5
0
0
Sum
19
76
26
105
15
112
178
180
105
20
Table 3: Forehand Mosquito Larval Trap Culex Eqq Rafts Trial 1
Culex rafts
S1
S2
S3 S4
Raft Total
Alfalfa
10
3
C
0
II
Pig
7
3
C
0
1C
Rice
28
5
C
2
3E
Timothy
2
1
C
0
a
Water
0
0
C
0
C
61
b
Table 4 stat. Forehand Mosquito Larval Trap Cuiex Egg Rafts Trial 1
ANOVA: Single Factor
SUMMARY
Groups
Alfalfa
Pig
Rice
Timothy
Water
Count
Sum Average
Variance
13
3.25
22.25
10
2.5
11
4
35
8.76 168.9167
4
3
0.75 0.916667
4
0
0
C
4
4
ANOVA
Source of Variation
Between Groups
Within Groups
SS
df
189.7
4
609.25
15
Total
798.95
MS
47.425
40.61667
F
P-value
F crit
1.167624 0.364112 3.055568
19
Table 5 stat. Forehand Mosquito Larval Trap Aedes Egg Counts Trial 1
SUMMARY
Groups
Alfalfa
Pig
Rice
Timothy
Count
4
4
4
4
Sum Average
Variance
0
0
C
43
10.75
358.25
35
8.75
106.25
93
23.25 956.9167
Source of Variation
Between Groups
Within Groups
SS
df
3
1103.18E
4264.2:
12
Total
5367.43E
MS
367.7292
355.3542
F
P-value
1.034824 0.412102
F crif
3.4903
15
Table 6: Forehand Mosquito Larval Trap Aedes Eag Counts Trial 1
S1
S2
S3 S4
Raft Total
Alfalfa
0
0
C
0
C
4
0
C
39
42
Pig
Rice
20
0
1E
0
3E
Timothy
26
I
C
66
92
Water
1
0
1
11
12
184
Table 7: Forehand Cone T r a Trial
~
2
Culex cyuinquefuscic~fxs
egg raft@)
Treatment Material Used
1Rice + Alfalfa
2 Rice
2Alfalfa
Rice + Alfalfa
+ Dibutyl
4succinate
ZWater-Control
Site
llrep
Site Site Site
Trt
2lrep 31rep 41rep total
C
2
9
3
14
S
18
9
4
4C
2
7
7
7
22
Table 8 stat. Forehand Cone Trap Trial 2
Culex auinqu~fascinttr.~
eua raft(s)
SUMMARY
Count Sum Average Variance
4 14
3.5
15
Rice + Alfalfa
4 40
10
34
Rice
Alfalfa
4 23
5.75
6.25
Rice + Atfatfa + Dibutyl succinate
4 32
8 7.333333
4
7
1.752.916667
Water-Control
Groups
ANOVA
Source of Variation
Between Groups
Within Groups
SS df
176.7
4
196.5 15
Total
373.2
MS
F
P-value F crif
44.175 3.372137 0.037048 3.055568
13.1
19
SUMMARY
Groups
Rice + Alfalfa + Dibutyl succinate
4 32
8 7.333333
Water-Control
4
7
1.752.916667
ANOVA
Source of Variation
Between Groups
Within Groups
Total
SS
df
78.125
1
30.75 6
108.87
5
MS
F
P-value F crit
78.125 15.2435 0.007944 5.987374
5.125
7
SUMMARY
Groups
Water-Control
Rice + Alfalfa
ANOVA
Source of Variation
Between Groups
Within Groups
Total
4
7
1.75 2.916667
4 14
3.5
15
SS
dY
MS
F
P-value F crit
6.125
1
6.125 0.683721 0.439946 5.987374
53.75 68.958333
59.875
7
GDF
GOVERNO DO DISTRITO FEDERAL
SECRETARIA DE ESTADO DE SAUDE
SUBSECRETARIA
DE VIGILANCIA A SAUDE
DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE
GERENCIA DE VETORES E ANIMAlS PECONHENTOS
NUCLEO DE PESQUISAS ENTOMOLOGICAS
E ANIMAlS
PECONHENTOS.
TESTES DE ARMADILHAS DO TIPO
BITE BACK NA CIDADE SATELITE
GAMA-DF
SAIN - Avenida Cont~rno Do Bosque Lote 04 - BRASiLIA - OF Telefone: 3342-3332
GDF
SUBSECRETARIA
DE VIGlLANCIA A SAUDE
GERENCIA DE VETO RES E ANIMAlS PEC:;ONHENTOS
E ANIMAlS
PEC:;ONHENTOS.
EQUIPE:
DR. ADELMO CARVALHO SOBRINHO: BIOLOGO CHEFE
SR. EZEQUIAS OLIVEIRA DA SILVA: AGENTE DE SAUDE
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SUBSECRETARIA
GERENCIA DE VETO RES E ANIMAlS PEC;ONHENTOS
E ANIMAlS
PEC;ONHENTOS.
EQUIPE:
DR. ADELMO CARVALHO SOBRINHO: BIOLOGO CHEFE
SR. EZEQUIAS OLIVEIRA DA SILVA: AGENTE DE SAODE
SAIN - Avenida
--------""--
Contorno
Do Bosque
Lote 04 - BRASiLIA
-----~
- OF Telefone:
3342-3332
GDF
SUBSECRETARIA DE VIGILANCIA A SAUDE
GERENCIA DE VETO RES E ANIMAlS PEGONHENTOS
NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PEGONHENTOS.
APRESENTACAO
HISTORICO DA ARMADILHA BITE BACK
Estudos
Depaliamento
preliminares
feitos
pelo
de Entomologia da Universidade da
Georgia demonstraram 0 potencial da Bite Back! a
1
armadilha
para
larva
de
mosquito
em
atrair
mosquitos femeas poedeiras e destruir suas larvas.
Um estudo aprofundado foi encomendado ao
Departamento
de Entomologia
Universidade
da
Florida
e Nematologia
sabre
Bite
Back!,
da
a
armadilha para larva de mosquito. A eficacia da
armadilha foi intensamente
avaliada.
No final do
teste de 30 dias, cada armadilha havia eliminado
mais de 1.000 mosquitos.
Foram utilizadas
20
armadilhas.
Testes independentes feitos pela Bite Back,
LLC
-
Limited
Liability
Company
em
areas
infestadas por mosquitos confirmaram a eficacia da
Funil
I
BMreir,_~de /'-"\._£~ - /""'>
As(en~ao ,,-!~.c_--:r,'~!::.'
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l..-.......-_":;':::"'-.:.:. .~
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Bite Back!, a armadilha para larva de mosquito.
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GDF
SECRETARIA DE ESTADO DE SALIDE
SUBSECRETARIA
DE VIGILANCIA A SALIDE
D1RETORIA DE VIGILANCIA AMBIENTAL EM SALIDE
GERENCIA DE VETORES E ANIMAlS PE<;ONHENTOS
NliCLEO DE PESQUISAS ENTOMOLOGICAS
E ANIMAlS
PE<;ONHENTOS.
INTRODUCAO
o
uso de armadilha para detectar a presenca de Aedes aegypti e
uma tecnica do tempo de Oswaldo Cruz (Cruz, 1909). Durante a campanha
iniciada em 1903, quando, numa area, a pesquisa larvaria tornava-se
negativa, eram colocadas vasilhas com agua em locais adequados para a
postura desse mosquito.
Aedes (Stegomyia) aegypti (Linnaeus, 1762) e Aedes (Stegomyia)
albopictus (Skuse, 1894) sac mosquitos vetores de arbovirus que infectam
0
homem e, no Brasil, infestam mais 5.000 municlpios, respectivamente.
Diversos estudos sobre ecologia, biologia, controle e descricao de criadouros
dessas especies tem side desenvolvidos.
Os criadouros preferenciais para Ae. aegypti e Ae. albopictus sac os
recipientes artificiais como: latas, vidros, vasos de cemiterio, caixa d'agua e
pneus vazios.
Dentre os criadouros artificiais, onde ambas as especles sac
encontradas, os pneus tem merecido atencao da vigilancia Entomol6gica por
apresentarem reproducao relevante desses mosquitos. Esses dep6sitos
conseguem armazenar grande quantidade de agua, proporcionam baixa
evaporacao e sac importantes artigos de comercio em nlvel nacional e
internacional.
A interacao do Ae. albopictus e Ae. aegypti requer atencao pois
essas especies se desenvolvem essencialmente nos mesmos criadouros
artificiais e sac muito comuns em areas de grande concentracao humana. 0
aumento da populacao e a expansao de Ae. albopictus em muitas areas esta
relacionado ao decHnio de Ae. aegypti. Em vista disso,
0
presente estudo se
GDF
SUBSECRETARIA
DE VIGIu\NCIA
A SAUDE
DIRETORIA DE VIGIu\NCIA
AMBIENTAL EM SAUDE
GERENCIA DE VETO RES E ANIMAlS PE<;ONHENTOS
E ANIMAlS
PE<;ONHENTOS.
propos a avaliar a frequemciade larvas, pupas (Ae. aegypti e Ae. albopictus)
e de outros mosquitos e verificar inftuencia de alguns fatores ffsicos e
ambientais na frequencia e preferencia
tipo "Bite Back".
dessas especies, em armadilhas
GDF
o
objetivo
SUBSECRETARIA
DIRETORIA DE VIGILANCIAAMBIENTAL
EM SAUDE
GERENCIA DE VETORES E ANIMAlS PE~ONHENTOS
E ANIMAlS
deste trabalho
sera
0
de identificar
PE~ONHENTOS.
a preferencia,
coexistencia e frequencia de Ae. aegypti e Ae. albopictus em armadilhas Bite
Back, em comparagao aos demais depositos como: pneus, latas, bacias,
vidros de cemiterio, caixa d'agua, criadouros estes onde ambas especies SaD
encontradas.
GDF
SUBSECRETARIA
E ANIMAlS
PE<;ONHENTOS.
Os pontos escolhidos na cidade satelite do Gama-DF em que
haviam indices de infestacao comprovada.
Os domicilios escolhidos dentro de criterios entomol6gicos e
distantes um do outro de no minimo 01 quil6metro.
Observadas as condicoes fisicas e ambientais de cada unidade a
ser pesquisada.
Foram instaladas 10 armadilhas, uma em cada domicilio; sendo que
a armadilha numero 5 foi utilizada como controle.
As armadilhas ficaram expostas por 29 dias consecutivos e foram
observadas semanalmente por urn agente de saude lotado na Inspetoria de
Saude do Gama, que conhece morfologia de Aedes aegypti e fez as
anotacoes das alteracoes de temperatura, clima e outros fatores, observando
a freqQencia de oviposicoes, larvas, pupas e mosquitos adultos, presos
nestas armadilhas.
As armadilhas foram instaladas
a
uma altura minima de 60
centimetros em locais sombreados, pr6ximos a plantas e a outros dep6sitos
____
~_SA_I_N__-_A_v_enidaContorno
Do Bosqu_e L_ote_04 - ~BR_A_SiL~- OF Te_lefone: 3342-3332
GDF
SUBSECRETARIA
E ANIMAlS
PECONHENTOS.
naturais e artificiais, onde foram observados criteriosamente a sua
preferencia por depositos e registrado a temperatura ambiente entre 26 e
32°, e umidade relativo do ar variando entre 45 e 95%. Foram utilizados 2
litros de agua por armadilha e com atrativo natural (feno), capim seco.
SAIN - Avenida
------~
Contorno
----
Do Bosque
Lote 04 - BRASiLIA
--~
--
- OF Telefone:
-
-
-
3342-3332
--
GDF
SAIN - Avenida
---------------
SECRETARIA DE ESTADO DE SAlIDE
SUBSECRETARIA
DE VIGILANCIA A SAlIDE
DIRETORIA DE VIGILANCIA AMBIENTAL EM SAlIDE
NlICLEO DE PESQUISAS ENTOMOLOGICAS
E ANIMAlS
PE<;ONHENTOS.
ANEXOS
Contorno
Do Bosque
-
Lote 04 - BRASiLIA
-
--
--
-
- OF Telefone:
-_.
-
3342-3332
--
GDF
SUBSECRETARIA
EM SAUDE
GERENCIA DE VETO RES E ANIMAlS PECONHENTOS
E ANIMAlS
MONITORAMENTO
PECONHENTOS.
E PESQUISA ENTOMOLCGICA
LOCALIZACAo:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK.
EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULlciDEOS
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICILlOS, 10 ARMADILHAS ENUMERADAS
EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA
DE 60 CM EM RELACAo AO SOLO
METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
RACAo PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS.
ATRATIVO: FENO (CAPIM SECO)
NOME DO MORADOR: ABiLlO
ENOERECO: QUADRA 19 CASA 17 SETOR OESTE
DATA DO INiclO DA EXPOSICAo:
---------
12/01/2009.
SAIN:- Aveiiida COiitorno Do Bosque Lote 04 - BRASiLIA - DF Telefoiie: 3342-3332
~
-
--------------
----
GDF
SUBSECRETARIA
DE VIGILANC'A A SAlIDE
EM SAlIDE
E ANIMAlS
MONITORAMENTO
PECONHENTOS.
E PESQUISA ENTOMOLDGICA
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULIciDEOS
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICfLlOS, 10 ARMADILHAS ENUMERADAS
DE 60 CM EM RELACAO AO SOLO
METOOOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
NOME DO MORAOOR: ADELMO
ENOERECO: QUADRA 25 CASA 52 SETOR LESTE
12/01/2009.
x
0
X
0
X
0
X
0
**NEG.
SAIN - Avenida
------------------
Contorno
Do Bosque
----
Lote 04 - BRASiLIA
-
-
- OF Telefone:
--------
QTD. LARV. CO LET.
3342-3332
--
-
-
--~
GDF
SUBSECRETARIA
GERENCIA DE VETO RES E ANIMAlS PEQONHENTOS
E ANIMAlS
MONITORAMENTO
PEQONHENTOS.
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
OBJETIVO: VERIFICAR A EFICAclA
DA ARMADILHA LARVITRAMPA TIPO BITE BACK.
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS ENUMERADAS
ALiMENTACAO:
RACAO PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS.
NOME DO MORAOOR: ANTONIO CARLOS
ENOERECO: QUADRA 08 CONJUNTO B CASA 04 SETOR CENTRAL
ARMADILHA N° 04 LOCAL DA EXPOSICAo:
VARANOA OA FRENTE
12/01/2009.
--
X
0
DATA DA VISITA SEMANAL:
20/01/2009.
--
X
0
DATA DA VISITA QUINZENAL:
30/ 01/ 2009.
X
--
9
--
X
0
DATA DO TERMINO DA EXP.:
09/ 02/ 2009.
*pos
**NEG.
QTD. lARV. eOlET.
SAIN - Aveiiida COiitorno Do Bosque Lote 04 - BRASiuA - OF Telefoiie: 3342-3332
GDF
SUBSECRETARIA
E ANIMAlS
MONITORAMENTO
PECONHENTOS.
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULICIDEOS
(MOSQUITOS)
METODOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
ATRATIVO:
RACAO PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS.
FENO (CAPIM SECO)
NOME DO MORADOR: CARMEM
ENDERECO: QUADRA 19 CASA 51 SETOR OESTE
VARANDA DOS FUNDOS
DATA DO INICIO DA EXPOSICAo:
12/01/2009.
--
X
0
20/ 01/ 2009.
--
X
0
30/01/2009.
X
--
5
09/02/2009.
--
X
0
*pos
---- SAIN - Avenida
**NEG.
QTD. lARV. eOlET.
Contorno Do Bosque Lote 04 - BRASiuA - OF Telefone: 3342-3332
GDF
SUBSECRETAR/A
DE V/G/LANC/A A SAlIDE
D/RETOR/A DE VIGILANCIA AMBIENTAL EM SAlIDE
GERENC/A DE VETO RES E ANIMAlS PECONHENTOS
NlICLEO DE PESQU/SAS ENTOMOLOGICAS
E ANIMAlS
MONITORAMENTO
PECONHENTOS.
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS
ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS,
APROXIMADAMENTE EM ALTURA DE 60 CM EM RELA<;Ao AO SOLO
METOOOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
RA<;Ao PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS.
NOME DO MORAOOR: EDMILSON
DATA DO INICIO DA EXPOSI<;AO: 12/01/2009.
GDF
SUBSECRETARIA
DIRETORIA DE VIGILANCIA AMBIENTAL EM SAlIDE
E ANIMAlS
MONITORAMENTO
PEC;ONHENTOS.
E PESQUISA ENTOMOLOGICA
LOCALIZACAO:
BRASIL:
BRASILIA:
CIDADE: GAMA-DF
(MOSQUITOS)
METODOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADAARMADILHA;
ALiMENTACAO:
ATRATIVO:
RACAo PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS.
FENO (CAPIM SECO)
NOME DO MORADOR: GELMA
ENDERECO: QUADRA A CONJUNTO 06 SETOR OESTE - VILA RORIZ
VARANDA
DOS FUDOS
12/01/2009.
--
X
0
20/01/2009.
X
--
12
30/01/2009.
--
X
0
09/ 02/ 2009.
X
--
21
*pos
**NEG.
QTD. lARV. eOlET.
GDF
SUBSECRETARIA
E ANIMAlS
MONITORAMENTO
PEC;ONHENTOS.
E PESQUISA ENTOMOLC>GICA
LOCALIZACAo:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
(MOSQUITOS)
METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADAARMADILHA;
ALiMENTACAO:
NOME DO MORAOOR: GISELA
ENOERECO: QUADRA A CONJUNTO 07 CASA 15 SETOR OESTE - VILA RORIZ
12/01/2009.
GDF
SUBSECRETARIA
E ANIMAlS
MONITORAMENTO
PE<;ONHENTOS.
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CUUCiDEOS
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICiuos,
10 ARMADILHAS ENUMERADAS
METODOS: 02 (DOIS) UTROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
RACAO PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS.
NOME DO MORADOR: MARIA DO CARMO
ENDERECO: QUADRA 27 CASA 06 SETOR OESTE
ARMADILHA N° 08 LOCAL DA EXPOSICAO: VARANDA DOS FUNDOS
DATA DO INiclO DA EXPOSICAO: 12/01/2009.
--
X
0
201 011 2009.
--
X
0
30101/2009.
X
--
3
09/02/2009.
X
--
8
*pos
**NEG.
QTD. lARV.
eo lET.
GDF
GOVERNO DO DISTRITO FEDERAlSECRETARIA DE ESTADO DE SAUDE
SUBSECRETAR/A
DE VIG/LANC/A A SAUDE
DIRETOR/A DE VIGILANCIA AMBIENTAL EM SAUDE
GERENCIA DE VETO RES E ANIMAlS PE<;ONHENTOS
E ANIMAlS
MONITORAMENTO
PE<;ONHENTOS.
LOCALIZACAo:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS ENUMERADAS
ALIMENTACAO:
ATRATIVO:
FENO (CAPIM SECO)
NOME 00 MORAOOR: NEUZA GUEDES
12/01/2009.
GDF
SECRETARIA DE ESTADO Q.E SAUDE
,
SUBSECRETARIA
GERENCIA DE VETO RES E ANIMAlS PEc;ONHENTOS
E ANIMAlS
PEc;ONHENTOS.
MONITORAMENTO E PESQUISA ENTOMOLOGICA
LOCALIZACAO:
BRASIL:
BRASiLIA:
CIDADE: GAMA-DF
EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CUUCIDEOS
(MOSQUITOS)
MONITORAMENTO: SERAo EXPOSTAS EM DOMICIUOS, 10 ARMADILHAS ENUMERADAS
METOOOS: 02 (DOIS) UTROS DE AGUA POTAvEL EM CADA ARMADILHA;
ALiMENTACAO:
ATRA TIVO: FENO (CAPIM SECO)
NOME DO MORAOOR: OSNAN
ENOERECO: QUADRA A CONJUNTO 05 CASA 22 SETOR OESTE - VILA RORIZ
QUINTAL FUNOOS
12/01/2009.
GDF
SUBSECRETARIA
E ANIMAlS
PECONHENTOS.
Os locais onde foram expostas as armadilhas havia diversos
depositos naturais e artificiais ja existentes. Com as armadilhas do tipo BITE
BACK colocadas proximo a esses depositos,
0
resultado da coleta nas
armadilhas obteve a seguinte configuracao:
Foram coletadas
e
identificadas
211
larvas
de culicfdeos.
Identificadas em laboratorio de entomologia do Gama-DF pelos tecnicos
MILTON LOPES COUTINHO e CRISPIM CARVALHO DA SILVA, onde
identificaram:
• 102 larvas de Aedes (stegomya) aegypti;
• 59 pupas de Aedes (stegomya) aegypti;
• 36 larvas do segundo estadio de Aedes (stegomya) aegypti;
• 08 larvas do terceiro do Aedes (stegomya) aegypti;
• 07 larvas do primeiro estadio do Aedes (stegomya) aegypti
... totalizando 211 especime deste vetor.
Foi capturado tambem na armadilha numero 03, cinco alados do
Aedes (stegomya) aegypti, sendo tres machos e duas femeas contidos
dentro da armadilha.
Nao foram encontradas larvas de Aedes (stegomya) albopictus nos
locais onde foram realizados os teste com armadilhas BITE BACK; ou seja,
nem nessas armadilhas, nem nos demais depositos proximos a essas
armadilhas.
SAIN - Avenida
Contorno
Do Bosque
Lote 04 - BRASiLIA
- OF Telefone:
------------------------------------
3342-3332
SUBSECRETARIA
DE VIGIlJ\NCIA A SAUDE
DIRETORIA DE VIGIlJ\NCIA AMBIENTAL EM SAUDE
E ANIMAlS
GDF
PECONHENTOS.
CONCLUSAO
Foram coletados
ovos,
larvas
e mosquitos
adultos
do Aedes
(stegomya) aegypti, exceto a armadilha numero cinco, pois a mesma tinha a
fun9ao de controle (sentinela); E nao foi reposta
recipiente pre to, restringindo
a agua,
que ficou abaixo do
assim a oviposi9ao dos culicfdeos e outros
artr6podas.
Conclufmos que estas armadilhas do tipo BITE BACK apresentamse como dep6sitos;
ou seja, uma boa armadilha
para culicfdeos
e em
especial para Aedes (stegomya) aegypti, onde mostrou uma eficiencia de
99% de atracao natural, como dep6sito preferencial.
Sugerimos
0
seu usa em area de baixa cobertura por agentes de
saude, servindo como uma boa opcao de vigil~mcia entomol6gica em areas
descobertas, pois ah§m da eficacia demonstrada nos testes, apresenta ainda
uma seguranca no seu formate estrutural e ffsico desta armadilha.
Este
e 0 relat6rio
Final.
DR. ADELMO CARVALHO SOBRINHO
Chefe do Nucleo de Pesquisas Entomol6gicas e Animais Peconhentos
do Distrito Federal

GDF - HBM Distribution

Transcrição

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