GDF - HBM Distribution
Transcrição
GDF - HBM Distribution
SIJMMARY SHEET OF MOSQ'IJITO LARVAL TRAP TESTING CONDUCTED AT THE UNIVERSITY OF FLORIDA Background: Experiments were conducted during the months of August, September, and November, 2003, utilizing the original patented mosquito larva traps (U.S. Patent No. 6,886,293). Subsequent to these and other tests, revisions were made that further enhanced the trapping abilities of the product (U.S. Patent No. 7,134,238). Objectives: After determining the proper test procedures, the objectives of the testing were to: 1) Ascertain trap effectiveness. 2) Identify potential stimulants to further attrac,t gravid mosquitoes. Methodology: Each test consisted of the deployment of twenty traps in various locations around the Entomology building on campus. Although this deployment in a small area diminished the attractant abilities of any single trap due to competition Aom nearby units, the overall results of the tests in relation to total kills and best stimulants verses the control units containing water only remain valid. The dedinitive test results are found in the second round of testing; the first round being utilized to refine the methods used in the second round. The results of this second testing are portrayed in graph form on page 15 of the attached report. The combined capture rate of the traps over a thirty-eight day period was estimated to be 17,400 mosquitoes or approximately 458 mosquitoes per day. (1 16 egg rafts (page 15) x 150 eggs per raft (page 3) = 17,400 mosquitoes) Summary: Gravid (egg bearing) mosquitoes generally live their entire lives, (approximately 15 days as adults), in an area about the size of a football field (less than '/z acre) and lay eggs 3 - 5 times during their adult Iife. The Bite Back! traps collect and destroy the offspring of the adults, thus breaking the exponential cycle of mosquito infestation in a given area during the breeding months. Field deployment of traps has proven the product to be highly effective in mosquito eradication while being safe to use around children and pets. With the stimulant being rice based, the Bite Back! trap uses no poisons or harmful chemicals. Rather than electrocution or death by poison, the Bite Back! trapping action relies on the natural tendencies of the mosquito larva to dive for protection and rest. Conclusion: This product is the only mosquito control system available today that is environmentally friendly, truly safe to use around children and pets, has been tested and proven effective in eliminating mosquito problems, and is inexpensive to deploy and maintain. >. _.Q. f.&$3:$/& UNIVERSITY OF , ; \ . .;.. - ,.,,., . + -:;s ,:,..,, z- FLORIDA Institute of Food and Agricultural Sciences Natural Area Drive PO Box 110620 Gainesville, F1 3261 1-0620 (352) 392-1901 Fax (352) 3924190 Entomology & Nernatology Department December 2,2003 James D. Forehand 42 Parkstone Court Stone Mountain, Georgia 30087 Dear Jimmy The Forehand Mosquito Larval Trap report is enclosed. /;I" J. F. But r, Professor Med. Vet. Entomology SUBJECT: Test Forehand Mosquito Larval Traps. PEST: Mosquitoes. TITLE: Forehand Mosquito Larval Trap Evaluations Trial 1 and 2. LEADER: Dr. J. F. Butler, Department of Entomology and Nematology, IFAS, University of Florida, Bld 970, Natural Area Drive Box 1 10620 Gainesville, Florida 326 11-0620. (352 392-1930-152) E-mail= jfl>@trnv.ifas.ufl .edu Dr. J. Maruniak Department of Entomology and Nematology, IFAS, University of Florida, Bld. 970, Natural Area Drive Box 110620 Gainesville, Florida 326 11-0620 COOPERATORS: James D. Forehand 42 Parkstone Court Stone Mountain, Georgia 30087 James Forehand" jdf~rehand~earthiin k .net OBJECTIVES: The objectives were: (1.) To develop testing procedures to evaluate mosquito traps. (2.) To determine the ef-fectiveness of the Forehand inverted cone mosquito larval trap. (3.) To add potential egg laying stimulants to the trap to improve egg and larval capture rate. MATERIALS and METHODS: Mosquito Larval Trap Trial 1: Forehand Mosquito LarvalJEgg Traps were evaluated with different types of media as an attractant for natural egg laying. Five treatments with four replications were used (20 traps). Traps were placed in four different areas near the Entomology and Nematology Building 970. Trap treatments were randomized with 4 replications per treatment. Traps were labeled with date, treatment number, and location with white tape placed on the trap side. A one by six cm strip of filter paper was placed in the cone for egg attachment. Medias evaluated in Trap 1 are as follows: Media 1: 2 Liters of water 2 grams of powdered Pot Bellied Pig Chow 0.5 gram of brewers yeast Media 2: 2 Liters of water 2 grams of powered Timothy Hay 0.5 gram of brewers yeast Media 3: 2 Liters of water 2 grams of powered Pure Rice Bran 0.5 gram of brewers yeast Media 4: 2 Liters of water 2 grams of powered Alfalfa Pellets 0.5 gram of brewers yeast Media 5: 2 Liters of water The traps with media were placed outside but protected from rain by a roof overhang. The windowsills of the inner courtyard and out side of building 970 were used. Observations were made after the first 7 days to determine if mosquitoes had begun laying eggs. The number of egg rafts present on the water surface as well as individual eggs placed on small blotter paper strips at the side of the cone leading into the water was monitored. After 5 weeks the residue in the traps was evaluated for mosquito larvae and other arthropods present. Adult mosquitoes in the upper trap area were monitored on a weekly basis. Mosquito Larval Trap Trial 2: Forehand Mosquito Larval Traps were set up as in Trial 1. Five treatments with four replications were used. The traps with media were placed outside of the windowsills in four different areas of building 970 in the inner courtyard. Evaluation of egg rafts and egg deposition were the only data monitored for Trial 2 as these criteria were found to be the most reliable data from Trial 1. The one by six cm strip of filter paper was placed in the cone for egg attachment and attractant placement. The strip was changed every 4 days. The following media were evaluated in Trial 2. Media 1: 1 gram of powered Pure Rice Bran 1 gram of powered Alfalfa Pellets 2 Liters of water 0.5 gram of brewers yeast Media 2: 2 Liters of water 2 grams of powered Pure Rice Bran 0.5 gram of brewers yeast in Media 3: 2 Liters of water 2 grams of powered Alfalfa Pellets 0.5 gram of brewers yeast Media 4: 1 gram of powered Pure Rice Bran 1 grams of powered Alfalfa Pellets 0.005 gram of attractant on the top of the attached iilter paper weekly 2 Liters of water 0.5 gram of brewers yeast Media 5: 2 Liters of water Observations were made as noted in the tables for the number of mosquito egg rafts present on the surface of the water and the individual eggs attached to the filter paper inserts. Previous counts of mosquito larvae and other insects present in Trial 1 were limited in value as the number of mosquitoes that died in the I" and 2nd instar stages could not be evaluated. The most valuable counts in Trial 1 were the number of egg rafts in the surface of the water and the number of individual eggs present on the attached paper strip. These gave a better evaluation of the potential mosquitoes exposed in the trap. A mosquitoe normally produces 2-3 egg rafts in her lifetime due to natural mortality rates. Therefore one egg raft or egg deposition represents about one third of their lifetime expected reproductive potential. A mosquito egg raft contains about 150 eggs. The observations of egg raft counts were timed to record the numbers prior to hatching and dispersal of 1"' instar larvae into the trap. Observations as egg and egg rafts were made daily for 2 weeks to determine the number of mosquito eggs laid. Adult mosquitoes surviving in the upper trap area were monitored on a weekly basis. RESULTS and DISCISSION: There were three objectives: (1.) To develop testing procedures to evaluate mosquito traps. (2.) To determine the effectiveness of the Forehand inverted cone mosquito larval trap. (3.) To evaluate an egg laying stimulants added to the trap. Trial 1 was a developmental step to determine how data could be collected for this new type of trap and to measure potential collection rates. As mosquitoes visiting and laying eggs in the trap could not be counted the egg rafts or individual eggs were counted within a time interval and summed over time for evaluation. As the media matured with fermentation the mosquito egg laying attraction generally increased (Figure 5). When the eggs hatched the small larvae generally migrated beneath the black funnel to the lighter area beneath and were trapped and drown A few first instar larvae were able to migrate through the small holes in the retention ring where they continued development to adults. As the media became older or about 2 weeks there was a debris buildup allowing some of the larvae to stay in the black funnel. We were not able to assess the numbers of larvae that were trapped and drown. The total number of mosquitoes present was estimated by the number of egg rafts, which were on the surface. An egg raft would result in 150 larvae for Clulex quuinyuqfu,~c~utus. Aedes ulhopictus females lay eggs singularly at or above the water line. These were assessed by placing 1 cm strips of blotter paper from the top of the trap into the water at the center. Success considered for A e h sp. was limited, as the number of eggs collected seemed low. I think that the surface of the shiny black funnel should be rough or non-glossy allowing for some moistening of the plasticlwater interface. Data collected for Trial 1 are presented in Tables 1-5. Results of Trial 1 are best shown in Figures 1-3. There were no significant differences in Trial 1 (Table 4,5) due to the placement of the replications on the outer windowsills of building 970. The mosquito populations on the outside of the building were not comparable with the inner courtyard. We were able to develop assay methods for the trapping system however, with egg rafts on the surface of the water and eggs placed upon the filter paper used to determine the number of potential mosquitoes developing. The presence and number of trapped insects were varied (Table 1). It was difficult to evaluate survivingldead insect numbers from those remaining and decaying in the trap so the number of eggs and egg rafts were adopted as a measure of potential mosquitoes exposed. Some adult mortality was seen in those insects trapped above the retention ring of the trap. The suitability of media in the traps was also observed. Table 1 gives the general observations on trap conditions and catches rates for traps during August 2003. In Trial 1 the condition and odor produced by the media was noted with the powered Pot Bellied Pig Chow and brewers yeast being particularly rank. This media would not be acceptable to the general public. This media is also not on the GRAS list. The other media were more acceptable and the ingredients are on the list. The media collecting the most C'ule,r quinqu~f~~sciutus was the Powered Pure Rice Bran and brewers yeast (Figure 2). This material could easily be packaged with the traps. Aedes eggs were highest for Powered Timothy hay and brewers yeast Figure I ). Powered Timothy hay is not on the GRAS list. The fermenting pig chow also attracted many other small Diptera maggots as demonstrated in Figure 3 and Table 1. Statistical dif'ferences could not be demonstrated in Trial 1 due to high variance in the replications presented between to outer windowsill and inner courtyard placement of traps. Trial 2 was more statistically reliable with replication placement allowing data collected to be analyzed. It also gave a measure of suitable media and augmentation of trap catch rates with potential attractants. Table 6-8 and Figures 4-6 presents these results. In this Trial powdered Rice Hulls and brewers yeast media were compared with powered Alfalfa Pellets and brewers yeast as well as their mixtures (Figure 5). The powdered Rice Hulls was significantly more attractive than Rice + Alfalfa + Dibutyl succinate which was significantly better than Alfalfa alone (Tables 6-8). The mixture of powdered Rice Hulls + powered Alfalfa Pellets were not significantly different than the water control. The mixtures of Rice Hulls + powered Alfalfa Pellets and Rice Hulls + powered Alfalfa Pellets + Dibutyl Succinate cannot be directly compared to the other treatments as the amount of each in the mixture was less than the other treatments. This was to maintain the same total 2 g amount of dry media at one gram of each in the mixture tested. The significant difference seen between the Rice Hulls + Alfalfa Pellets and Rice Hulls + Alfalfa Pellets + Dibutyl Succinate demonstrated that Dibutyl Succinate increased the catch rates for the traps. When Dibutyl Succinate was added to the filter paper on the top of the trap (0.005 g) the number of C'ulex yuinyu~firsc~utu.s egg rafts were doubled indicating that this material is a good attractant for stimulation of egg laying (Figure 5). Media conditioning and longevity is noted in Figure 5. A delay of 12 days was noted before mosquitoes were attracted to the traps. By day 29 the media had lost its major attractiveness. Traps will need to be serviced about once per month, cleaning out the debris and adding new water and media. SUMMARY: The powdered rice hulls at 2 grams + 0.5 g brewers yeast, in 2 Liters of water was the best both in numbers of eggs laid and suitability of product odor. One loading of a trap is good for about 30 days and then productivity goes to near 0. Our best data collection was a count of mosquito egg rafts (150 eggsiraft), as larval mortality could not be reliably estimated. We had some survival of mosquito larvae to adults during the test. The traps may need to be modified and or disturbed to assure trapping of all the larvae. I think that 6 small 2 cm no return cones with 3mm holes placed in the positioning ring (the ring just under the black funnel) would allow larvae to move into this area to breath air but be unable to return to the center exit of the trap. Larva in this region of the trap would survive to adult and then die allowing a precise trapping measure of numbers and species. This would also be a visible demonstration of adult mosquitoes being trapped and killed for the consumer. The water levels in the traps will also need to be maintained to keep traps effective. The trap that included Dibutyl Succinate as an attractant demonstrated double the catch rate of one gram each of Rice Hulls and Alfalfa. I assume that it would also work for rice hulls alone. The mosquitoes, which are the vectors of West Nile Virus (Culex yuinquqfiscicrtus), were the majority those trapped. Aedes mosquitoes were not attracted to laying eggs on the slick black surface and only laid a few eggs on the filter paper installed for the purpose. The shiny black surface should be converted to a mat or brushed surface to one centimeter above the water line, which may increase the egg laying for these mosquitoes. Table 1: Forehand Mosquito Larval cjeneral observations of Insects Collected and Media Conditions in T r a ~ s . a - alfalfa p - Pot Bellied Pig Chow r - powered Pure Rice Bran t - Timothy hay W- water (control) la (translucent green, neutral smell) I egg raft 9- 10 each 2-3rd instars larvae (dead) 1 each 4th instar mosquito Larva (dead) I p (near putrid) 1 egg raft 1 adult Culex yuinyuef~~rc~atus adult female 1 Pupa 25 dead adult C'x y uiny ztejusciatus. 4 each 4th instar C'x. yurnquefa.scratus larvae 2 each 3rd instar larvae Estimated 65 2nd instar larvae 1r (putrid, brown milky color) 40 each 2-3rd instar mosq larvae 1 sewer fly, Psychodidae 1 fruit fly, Drosophilidae 2 adult 0.yuinquefasciutus females, 1 male 1t (not putrid, translucent brown) 1 egg raft 9 Chironomid larvae 1 Psychodidae adult (dead) 1w (clear water) 3 Culicid larvae 4 Chironomidae larvae 2 each 4th instar (:x. y uinquefu.~cratus 2a (brownish-green, slight musky smell) 80 each 2-3rd instar mosquito larvae 2p (brown putrid) 1 pupal cast skin mosq 84 each Chironomid cases on clear plastic part of trap 9 each Chironomid cases on black lip 2 adult female Ck. yuinyuefusciutus, I male 2r (brown putrid) 1 egg raft 2 each 4th instar mosquito larvae 10 each 2-3rd instar mosquito larvae 2 Chironomid larvae (bloodworms) 2t (brown, slight non-putrid odor) Collembola; about 10 each dead on water 1 Psychodidae adult dead 1 Psychodidae larva 2w nothing Site 1 - inner courtyard, west Site 2 - inner courtyard, east Site 3 - outer courtyard, west Site 4 - outer courtyard, east 3a (heavily green, lots of algae) 1 each 1st instar mosquito larva 1 adult female Clx. qurnyuefr.~ciatus 3p (green water, nasty putrid) I each 2nd instar mosquito larva (dead) 3r (green water, slight odor) 98 each 2-4th instar mosquito larvae (dead) 3t (green, slight odor) nothing 3w (some algae, very slight) nothing 4a (brown, moderate stink) 1 each adult Culex (escaped) 3 each 4th instar larvae 15 pupae 75 each 4th instar C'x. quinyucfr.vcrutus 4p (putrid brown) 1 mosquito pupa Approximately 100 Chironomid larval cases 4r (putrid brown, orange fjlm on bottom of trap) 26 each 3-4th instar mosquito larvae Cx. quingu~f~~~crutus. Several dead Collembola 4t (brown, some odor) 105 each mosquito larvae, 2-4th instar C'x. yuinqu~fuscnrtus 50 immature cyclorraphids 15 mosq larvae, 2-4th instar, C'x. Qurnyu~fuscrutus 4w Table 2: Forehand Mosquito Larval Traps Trial 1 Mosauito Larvae Present in Traps Media tested S1 S2 S3 S4 11 80 2 Alfalfa Pig 98 1 3 Rice 42 14 98 Timothy 0 0 0 Water 5 0 0 Sum 19 76 26 105 15 112 178 180 105 20 Table 3: Forehand Mosquito Larval Trap Culex Eqq Rafts Trial 1 Culex rafts S1 S2 S3 S4 Raft Total Alfalfa 10 3 C 0 II Pig 7 3 C 0 1C Rice 28 5 C 2 3E Timothy 2 1 C 0 a Water 0 0 C 0 C 61 b Table 4 stat. Forehand Mosquito Larval Trap Cuiex Egg Rafts Trial 1 ANOVA: Single Factor SUMMARY Groups Alfalfa Pig Rice Timothy Water Count Sum Average Variance 13 3.25 22.25 10 2.5 11 4 35 8.76 168.9167 4 3 0.75 0.916667 4 0 0 C 4 4 ANOVA Source of Variation Between Groups Within Groups SS df 189.7 4 609.25 15 Total 798.95 MS 47.425 40.61667 F P-value F crit 1.167624 0.364112 3.055568 19 Table 5 stat. Forehand Mosquito Larval Trap Aedes Egg Counts Trial 1 ANOVA: Single Factor SUMMARY Groups Alfalfa Pig Rice Timothy Count 4 4 4 4 Sum Average Variance 0 0 C 43 10.75 358.25 35 8.75 106.25 93 23.25 956.9167 Source of Variation Between Groups Within Groups SS df 3 1103.18E 4264.2: 12 Total 5367.43E MS 367.7292 355.3542 F P-value 1.034824 0.412102 F crif 3.4903 15 Table 6: Forehand Mosquito Larval Trap Aedes Eag Counts Trial 1 S1 S2 S3 S4 Raft Total Alfalfa 0 0 C 0 C 4 0 C 39 42 Pig Rice 20 0 1E 0 3E Timothy 26 I C 66 92 Water 1 0 1 11 12 184 Table 7: Forehand Cone T r a Trial ~ 2 Culex cyuinquefuscic~fxs egg raft@) Treatment Material Used 1Rice + Alfalfa 2 Rice 2Alfalfa Rice + Alfalfa + Dibutyl 4succinate ZWater-Control Site llrep Site Site Site Trt 2lrep 31rep 41rep total C 2 9 3 14 S 18 9 4 4C 2 7 7 7 22 Table 8 stat. Forehand Cone Trap Trial 2 Culex auinqu~fascinttr.~ eua raft(s) ANOVA: Single Factor SUMMARY Count Sum Average Variance 4 14 3.5 15 Rice + Alfalfa 4 40 10 34 Rice Alfalfa 4 23 5.75 6.25 Rice + Atfatfa + Dibutyl succinate 4 32 8 7.333333 4 7 1.752.916667 Water-Control Groups ANOVA Source of Variation Between Groups Within Groups SS df 176.7 4 196.5 15 Total 373.2 MS F P-value F crif 44.175 3.372137 0.037048 3.055568 13.1 19 ANOVA: Single Factor SUMMARY Count Sum Average Variance Groups Rice + Alfalfa + Dibutyl succinate 4 32 8 7.333333 Water-Control 4 7 1.752.916667 ANOVA Source of Variation Between Groups Within Groups Total SS df 78.125 1 30.75 6 108.87 5 MS F P-value F crit 78.125 15.2435 0.007944 5.987374 5.125 7 ANOVA: Single Factor SUMMARY Groups Water-Control Rice + Alfalfa ANOVA Source of Variation Between Groups Within Groups Total Count Sum Average Variance 4 7 1.75 2.916667 4 14 3.5 15 SS dY MS F P-value F crit 6.125 1 6.125 0.683721 0.439946 5.987374 53.75 68.958333 59.875 7 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PECONHENTOS. TESTES DE ARMADILHAS DO TIPO BITE BACK NA CIDADE SATELITE GAMA-DF SAIN - Avenida Cont~rno Do Bosque Lote 04 - BRASiLIA - OF Telefone: 3342-3332 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGlLANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEC:;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PEC:;ONHENTOS. EQUIPE: DR. ADELMO CARVALHO SOBRINHO: BIOLOGO CHEFE SR. EZEQUIAS OLIVEIRA DA SILVA: AGENTE DE SAUDE GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEC;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PEC;ONHENTOS. EQUIPE: DR. ADELMO CARVALHO SOBRINHO: BIOLOGO CHEFE SR. EZEQUIAS OLIVEIRA DA SILVA: AGENTE DE SAODE SAIN - Avenida --------""-- Contorno Do Bosque Lote 04 - BRASiLIA -----~ - OF Telefone: 3342-3332 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEGONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PEGONHENTOS. APRESENTACAO HISTORICO DA ARMADILHA BITE BACK Estudos Depaliamento preliminares feitos pelo de Entomologia da Universidade da Georgia demonstraram 0 potencial da Bite Back! a 1 armadilha para larva de mosquito em atrair mosquitos femeas poedeiras e destruir suas larvas. Um estudo aprofundado foi encomendado ao Departamento de Entomologia Universidade da Florida e Nematologia sabre Bite Back!, da a armadilha para larva de mosquito. A eficacia da armadilha foi intensamente avaliada. No final do teste de 30 dias, cada armadilha havia eliminado mais de 1.000 mosquitos. Foram utilizadas 20 armadilhas. Testes independentes feitos pela Bite Back, LLC - Limited Liability Company em areas infestadas por mosquitos confirmaram a eficacia da Funil I BMreir,_~de /'-"\._£~ - /""'> As(en~ao ,,-!~.c_--:r,'~!::.' ,) l..-.......-_":;':::"'-.:.:. .~ ~....:_-~_:;-./ :(7~-~~-~~~"-""'--2\ Bite Back!, a armadilha para larva de mosquito. \~~32~~~:-c.:.-'=::d/f \ t I " \, ,-- 'B~iide . -- -------------------- ~ : GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SALIDE SUBSECRETARIA DE VIGILANCIA A SALIDE D1RETORIA DE VIGILANCIA AMBIENTAL EM SALIDE GERENCIA DE VETORES E ANIMAlS PE<;ONHENTOS NliCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PE<;ONHENTOS. INTRODUCAO o uso de armadilha para detectar a presenca de Aedes aegypti e uma tecnica do tempo de Oswaldo Cruz (Cruz, 1909). Durante a campanha iniciada em 1903, quando, numa area, a pesquisa larvaria tornava-se negativa, eram colocadas vasilhas com agua em locais adequados para a postura desse mosquito. Aedes (Stegomyia) aegypti (Linnaeus, 1762) e Aedes (Stegomyia) albopictus (Skuse, 1894) sac mosquitos vetores de arbovirus que infectam 0 homem e, no Brasil, infestam mais 5.000 municlpios, respectivamente. Diversos estudos sobre ecologia, biologia, controle e descricao de criadouros dessas especies tem side desenvolvidos. Os criadouros preferenciais para Ae. aegypti e Ae. albopictus sac os recipientes artificiais como: latas, vidros, vasos de cemiterio, caixa d'agua e pneus vazios. Dentre os criadouros artificiais, onde ambas as especles sac encontradas, os pneus tem merecido atencao da vigilancia Entomol6gica por apresentarem reproducao relevante desses mosquitos. Esses dep6sitos conseguem armazenar grande quantidade de agua, proporcionam baixa evaporacao e sac importantes artigos de comercio em nlvel nacional e internacional. A interacao do Ae. albopictus e Ae. aegypti requer atencao pois essas especies se desenvolvem essencialmente nos mesmos criadouros artificiais e sac muito comuns em areas de grande concentracao humana. 0 aumento da populacao e a expansao de Ae. albopictus em muitas areas esta relacionado ao decHnio de Ae. aegypti. Em vista disso, 0 presente estudo se GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGIu\NCIA A SAUDE DIRETORIA DE VIGIu\NCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PE<;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PE<;ONHENTOS. propos a avaliar a frequemciade larvas, pupas (Ae. aegypti e Ae. albopictus) e de outros mosquitos e verificar inftuencia de alguns fatores ffsicos e ambientais na frequencia e preferencia tipo "Bite Back". dessas especies, em armadilhas GDF o objetivo GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIAAMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PE~ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS deste trabalho sera 0 de identificar PE~ONHENTOS. a preferencia, coexistencia e frequencia de Ae. aegypti e Ae. albopictus em armadilhas Bite Back, em comparagao aos demais depositos como: pneus, latas, bacias, vidros de cemiterio, caixa d'agua, criadouros estes onde ambas especies SaD encontradas. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PE<;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PE<;ONHENTOS. Os pontos escolhidos na cidade satelite do Gama-DF em que haviam indices de infestacao comprovada. Os domicilios escolhidos dentro de criterios entomol6gicos e distantes um do outro de no minimo 01 quil6metro. Observadas as condicoes fisicas e ambientais de cada unidade a ser pesquisada. Foram instaladas 10 armadilhas, uma em cada domicilio; sendo que a armadilha numero 5 foi utilizada como controle. As armadilhas ficaram expostas por 29 dias consecutivos e foram observadas semanalmente por urn agente de saude lotado na Inspetoria de Saude do Gama, que conhece morfologia de Aedes aegypti e fez as anotacoes das alteracoes de temperatura, clima e outros fatores, observando a freqQencia de oviposicoes, larvas, pupas e mosquitos adultos, presos nestas armadilhas. As armadilhas foram instaladas a uma altura minima de 60 centimetros em locais sombreados, pr6ximos a plantas e a outros dep6sitos ____ ~_SA_I_N__-_A_v_enidaContorno Do Bosqu_e L_ote_04 - ~BR_A_SiL~- OF Te_lefone: 3342-3332 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGlLANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PECONHENTOS. naturais e artificiais, onde foram observados criteriosamente a sua preferencia por depositos e registrado a temperatura ambiente entre 26 e 32°, e umidade relativo do ar variando entre 45 e 95%. Foram utilizados 2 litros de agua por armadilha e com atrativo natural (feno), capim seco. SAIN - Avenida ------~ Contorno ---- Do Bosque Lote 04 - BRASiLIA --~ -- - OF Telefone: - - - 3342-3332 -- GDF SAIN - Avenida --------------- GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAlIDE SUBSECRETARIA DE VIGILANCIA A SAlIDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAlIDE GERENCIA DE VETORES E ANIMAlS PE<;ONHENTOS NlICLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PE<;ONHENTOS. ANEXOS Contorno Do Bosque - Lote 04 - BRASiLIA - -- -- - - OF Telefone: -_. - 3342-3332 -- GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIAAMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PECONHENTOS. E PESQUISA ENTOMOLCGICA LOCALIZACAo: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULlciDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICILlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RACAo PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORADOR: ABiLlO ENOERECO: QUADRA 19 CASA 17 SETOR OESTE DATA DO INiclO DA EXPOSICAo: --------- 12/01/2009. SAIN:- Aveiiida COiitorno Do Bosque Lote 04 - BRASiLIA - DF Telefoiie: 3342-3332 ~ - -------------- ---- GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAlIDE SUBSECRETARIA DE VIGILANC'A A SAlIDE DIRETORIA DE VIGILANCIAAMBIENTAL EM SAlIDE GERENCIA DE VETORES E ANIMAlS PECONHENTOS NlICLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PECONHENTOS. E PESQUISA ENTOMOLDGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULIciDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICfLlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAO AO SOLO METOOOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RACAo PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORAOOR: ADELMO ENOERECO: QUADRA 25 CASA 52 SETOR LESTE DATA DO INiclO DA EXPOSICAo: 12/01/2009. x 0 X 0 X 0 X 0 **NEG. SAIN - Avenida ------------------ Contorno Do Bosque ---- Lote 04 - BRASiLIA - - - OF Telefone: -------- QTD. LARV. CO LET. 3342-3332 -- - - --~ GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEQONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PEQONHENTOS. E PESQUISA ENTOMOLCGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULIciDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAO AO SOLO METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RACAO PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORAOOR: ANTONIO CARLOS ENOERECO: QUADRA 08 CONJUNTO B CASA 04 SETOR CENTRAL ARMADILHA N° 04 LOCAL DA EXPOSICAo: VARANOA OA FRENTE DATA DO INiclO DA EXPOSICAo: 12/01/2009. -- X 0 DATA DA VISITA SEMANAL: 20/01/2009. -- X 0 DATA DA VISITA QUINZENAL: 30/ 01/ 2009. X -- 9 -- X 0 DATA DO TERMINO DA EXP.: 09/ 02/ 2009. *pos **NEG. QTD. lARV. eOlET. SAIN - Aveiiida COiitorno Do Bosque Lote 04 - BRASiuA - OF Telefoiie: 3342-3332 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGlLANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PECONHENTOS. E PESQUISA ENTOMOLCGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULICIDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICILlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METODOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: ATRATIVO: RACAO PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS. FENO (CAPIM SECO) NOME DO MORADOR: CARMEM ENDERECO: QUADRA 19 CASA 51 SETOR OESTE ARMADILHA N° 01 LOCAL DA EXPOSICAo: VARANDA DOS FUNDOS DATA DO INICIO DA EXPOSICAo: 12/01/2009. -- X 0 DATA DA VISITA SEMANAL: 20/ 01/ 2009. -- X 0 DATA DA VISITA QUINZENAL: 30/01/2009. X -- 5 DATA DO TERMINO DA EXP.: 09/02/2009. -- X 0 *pos ---- SAIN - Avenida **NEG. QTD. lARV. eOlET. Contorno Do Bosque Lote 04 - BRASiuA - OF Telefone: 3342-3332 GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAlIDE SUBSECRETAR/A DE V/G/LANC/A A SAlIDE D/RETOR/A DE VIGILANCIA AMBIENTAL EM SAlIDE GERENC/A DE VETO RES E ANIMAlS PECONHENTOS NlICLEO DE PESQU/SAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PECONHENTOS. E PESQUISA ENTOMOLCGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULICIDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELA<;Ao AO SOLO METOOOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RA<;Ao PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORAOOR: EDMILSON ENOERECO: QUADRA 22 CONJUNTO B CASA 22 SETOR CENTRAL DATA DO INICIO DA EXPOSI<;AO: 12/01/2009. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAlIDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAlIDE GERENCIA DE VETO RES E ANIMAlS PEC;ONHENTOS NlICLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PEC;ONHENTOS. E PESQUISA ENTOMOLOGICA LOCALIZACAO: BRASIL: BRASILIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULICIDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICILlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METODOS: 02 (DO IS) L1TROS DE AGUA POTAvEL EM CADAARMADILHA; ALiMENTACAO: ATRATIVO: RACAo PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS. FENO (CAPIM SECO) NOME DO MORADOR: GELMA ENDERECO: QUADRA A CONJUNTO 06 SETOR OESTE - VILA RORIZ ARMADILHA N° 02 LOCAL DA EXPOSICAo: VARANDA DOS FUDOS DATA DO INICIO DA EXPOSICAo: 12/01/2009. -- X 0 DATA DA VISITA SEMANAL: 20/01/2009. X -- 12 DATA DA VISITA QUINZENAL: 30/01/2009. -- X 0 DATA DO TERMINO DA EXP.: 09/ 02/ 2009. X -- 21 *pos **NEG. QTD. lARV. eOlET. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEC;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PEC;ONHENTOS. E PESQUISA ENTOMOLC>GICA LOCALIZACAo: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULICIDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICILlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAO AO SOLO METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADAARMADILHA; ALiMENTACAO: RACAo PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORAOOR: GISELA ENOERECO: QUADRA A CONJUNTO 07 CASA 15 SETOR OESTE - VILA RORIZ DATA DO INICIO DA EXPOSICAo: 12/01/2009. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETORES E ANIMAlS PE<;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PE<;ONHENTOS. E PESQUISA ENTOMOLOGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CUUCiDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICiuos, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METODOS: 02 (DOIS) UTROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RACAO PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS. ATRATIVO: FENO (CAPIM SECO) NOME DO MORADOR: MARIA DO CARMO ENDERECO: QUADRA 27 CASA 06 SETOR OESTE ARMADILHA N° 08 LOCAL DA EXPOSICAO: VARANDA DOS FUNDOS DATA DO INiclO DA EXPOSICAO: 12/01/2009. -- X 0 DATA DA VISITA SEMANAL: 201 011 2009. -- X 0 DATA DA VISITA QUINZENAL: 30101/2009. X -- 3 DATA DO TERMINO DA EXP.: 09/02/2009. X -- 8 *pos **NEG. QTD. lARV. eo lET. GDF GOVERNO DO DISTRITO FEDERAlSECRETARIA DE ESTADO DE SAUDE SUBSECRETAR/A DE VIG/LANC/A A SAUDE DIRETOR/A DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PE<;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS MONITORAMENTO PE<;ONHENTOS. E PESQUISA ENTOMOLOGICA LOCALIZACAo: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CULIciDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICiLlOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METOOOS: 02 (DOIS) L1TROS DE AGUA POTAvEL EM CADA ARMADILHA; ALIMENTACAO: ATRATIVO: RACAo PARA PEIXE DILUiDA EM AGUA 05 (CINCO) GOTAS. FENO (CAPIM SECO) NOME 00 MORAOOR: NEUZA GUEDES ENOERECO: QUADRA 07 CONJUNTO B CASA 16 SETOR CENTRAL DATA DO INiclO DA EXPOSICAo: 12/01/2009. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO Q.E SAUDE , SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PEc;ONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PEc;ONHENTOS. MONITORAMENTO E PESQUISA ENTOMOLOGICA LOCALIZACAO: BRASIL: BRASiLIA: CIDADE: GAMA-DF OBJETIVO: VERIFICAR A EFICAclA DA ARMADILHA LARVITRAMPA TIPO BITE BACK. EXPERIMENTO: TESTE DE EFETIVIDADE DAS FORMAS IMATURAS DE CUUCIDEOS (MOSQUITOS) MONITORAMENTO: SERAo EXPOSTAS EM DOMICIUOS, 10 ARMADILHAS ENUMERADAS EM LOCAlS SOMBREADOS, PROXIMOS AS PLANTAS, APROXIMADAMENTE EM ALTURA DE 60 CM EM RELACAo AO SOLO METOOOS: 02 (DOIS) UTROS DE AGUA POTAvEL EM CADA ARMADILHA; ALiMENTACAO: RACAo PARA PEIXE DILUIDA EM AGUA 05 (CINCO) GOTAS. ATRA TIVO: FENO (CAPIM SECO) NOME DO MORAOOR: OSNAN ENOERECO: QUADRA A CONJUNTO 05 CASA 22 SETOR OESTE - VILA RORIZ ARMADILHA N° 10 LOCAL DA EXPOSICAo: DATA DO INICIO DA EXPOSICAo: QUINTAL FUNOOS 12/01/2009. GDF GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGILANCIA A SAUDE DIRETORIA DE VIGILANCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS PECONHENTOS. Os locais onde foram expostas as armadilhas havia diversos depositos naturais e artificiais ja existentes. Com as armadilhas do tipo BITE BACK colocadas proximo a esses depositos, 0 resultado da coleta nas armadilhas obteve a seguinte configuracao: Foram coletadas e identificadas 211 larvas de culicfdeos. Identificadas em laboratorio de entomologia do Gama-DF pelos tecnicos MILTON LOPES COUTINHO e CRISPIM CARVALHO DA SILVA, onde identificaram: • 102 larvas de Aedes (stegomya) aegypti; • 59 pupas de Aedes (stegomya) aegypti; • 36 larvas do segundo estadio de Aedes (stegomya) aegypti; • 08 larvas do terceiro do Aedes (stegomya) aegypti; • 07 larvas do primeiro estadio do Aedes (stegomya) aegypti ... totalizando 211 especime deste vetor. Foi capturado tambem na armadilha numero 03, cinco alados do Aedes (stegomya) aegypti, sendo tres machos e duas femeas contidos dentro da armadilha. Nao foram encontradas larvas de Aedes (stegomya) albopictus nos locais onde foram realizados os teste com armadilhas BITE BACK; ou seja, nem nessas armadilhas, nem nos demais depositos proximos a essas armadilhas. SAIN - Avenida Contorno Do Bosque Lote 04 - BRASiLIA - OF Telefone: ------------------------------------ 3342-3332 GOVERNO DO DISTRITO FEDERAL SECRETARIA DE ESTADO DE SAUDE SUBSECRETARIA DE VIGIlJ\NCIA A SAUDE DIRETORIA DE VIGIlJ\NCIA AMBIENTAL EM SAUDE GERENCIA DE VETO RES E ANIMAlS PECONHENTOS NUCLEO DE PESQUISAS ENTOMOLOGICAS E ANIMAlS GDF PECONHENTOS. CONCLUSAO Foram coletados ovos, larvas e mosquitos adultos do Aedes (stegomya) aegypti, exceto a armadilha numero cinco, pois a mesma tinha a fun9ao de controle (sentinela); E nao foi reposta recipiente pre to, restringindo a agua, que ficou abaixo do assim a oviposi9ao dos culicfdeos e outros artr6podas. Conclufmos que estas armadilhas do tipo BITE BACK apresentamse como dep6sitos; ou seja, uma boa armadilha para culicfdeos e em especial para Aedes (stegomya) aegypti, onde mostrou uma eficiencia de 99% de atracao natural, como dep6sito preferencial. Sugerimos 0 seu usa em area de baixa cobertura por agentes de saude, servindo como uma boa opcao de vigil~mcia entomol6gica em areas descobertas, pois ah§m da eficacia demonstrada nos testes, apresenta ainda uma seguranca no seu formate estrutural e ffsico desta armadilha. Este e 0 relat6rio Final. DR. ADELMO CARVALHO SOBRINHO Chefe do Nucleo de Pesquisas Entomol6gicas e Animais Peconhentos do Distrito Federal