1 EUPLAN Platelet Conference
Transcrição
1 EUPLAN Platelet Conference
1st EUPLAN Platelet Conference th September 19-21th 2012, Grand Hotel de l’Empereur Maastricht, The Netherlands WELCOME MESSAGE A warm welcome to Maastricht, one of the oldest cities in the Netherlands with a southern atmosphere, for this first meeting of the European Platelet Network (EUPLAN) in Grand Hôtel de l’Empereur. The program, including a special clinical session, is very international and exciting with 36 oral presentations and 98 poster presentations. Europe has a strong history in platelet research with meetings at a regular base since 1983, especially in Germany, the United Kingdom, Italy and France. In addition, the recently revitalized European Thrombosis Research Organization (ETRO) and the various national Thrombosis and Haemostasis Societies are active in stimulating and facilitating platelet research. The EUPLAN initiative started in October 2009 in Toulouse, when leading platelet scientists agreed that there is a need for regular meetings on a European level to exchange ideas and to support high quality basic and clinical research on platelets and megakaryocytes, next to the existing national meetings. However, the EUPLAN initiative goes further than only organizing conferences (see website http://euplan.eu/). The undertaking of the conference has been made possible by the generous support of our sponsors (see previous and next pages). We are very grateful for this. Please, visit their displays during the meeting. We hope that you have a pleasant stay in Maastricht. Johan Heemskerk and Judith Cosemans (on behalf of the organizing committee) Local organizing committee Contact EUPLAN Platelet conference Dr. Judith Cosemans Prof. dr. Hugo ten Cate Prof. dr. Johan Heemskerk Dr. Roosje van Gorp Department of Biochemistry, CARIM Maastricht University P.O. Box 616 6200 MD Maastricht The Netherlands Tel. +31-43-3881671 (3881674) Fax. +31-43-3884159 Department of Biochemistry, CARIM Maastricht University P.O. Box 616 6200 MD Maastricht The Netherlands Tel. +31-43-3881674 (3881671) Fax. +31-43-3884159 [email protected] or [email protected] http://euplan.eu/ 1 GOLD SPONSORS SILVER SPONSORS 2 SILVER SPONSORS BRONZE SPONSORS 3 KEYNOTE & GUEST SPEAKERS INTERNATIONAL SCIENTIFIC ADVISORY COMMITTEE Prof. dr. Marco Cattaneo Medicina III, Ospedale San Paolo Università di Milano, Italy Prof. dr. Hans Deckmyn Laboratory for Thrombosis Research KU Leuven, Campus Kortrijk, Belgium Prof. dr. Scott Diamond Institute for Medicine and Engineering University of Pennsylvania Philadelphia, USA Prof. dr. Christian Gachet INSERM UdS, EFS Alsace Strasbourg, France Dr. Laura Gutièrrez Sanquin-CLB Amsterdam, The Netherlands Dr. Kathleen Freson Center for Molecular and Vascular Biology University of Leuven, Belgium Prof. dr. Tomas Lindahl Clinical Chemistry Linköping University Hospital Linköping, Sweden Dr. Christian Hackeng Department of Clinical Pharmacy St. Antonius Hospital Nieuwegein, The Netherlands Prof. dr. Bernhard Nieswandt Rudolf Virchow Centre Julius Maximilians University Würzburg Germany Prof. dr. Paul Harrison Haemophilia and Thrombosis Centre Churchill Hospital Oxford, United Kingdom Prof. dr. Peter Presek Clinical Pharmacology Martin Luther University Halle-Wittenberg Halle, Germany Prof. dr. Berend Isermann Internal Medicine & Clinical Chemistry University of Heidelberg Heidelberg, Germany Prof. dr. Mauro Torti Department of Biochemistry University of Pavia, Pavia, Italy Dr. Mark Roest Clinical Chemistry & Hematology Utrecht Medical Center, The Netherlands Prof. dr. William Vainchenker Institute Gustave Roussy UMR1009, Villejuif France Dr. Albert Smolenski Conway Institute University College Dublin, Ireland Prof. dr. Thomas Unger Cardiovascular Research Institute Maastricht (CARIM) Maastricht, The Netherlands Dr. Ivo Volf Institute of Physiology Center for Physiology & Pharmacology Medical University of Vienna Vienna, Austria Prof. dr. William Vainchenker Institute Gustave Roussy UMR1009, Villejuif, France Prof. dr. Steve Watson Cardiovascular & Cellular Pharmacology Medical School, University of Birmingham United Kingdom Prof. dr. Steve Watson Cardiovascular & Cellular Pharmacology Medical School, University of Birmingham United Kingdom 4 SCIENTIFIC PROGRAMME Wednesday, 19th September 2012 13.30 - 17.30 Special methods workshops (only for participants registered for the workshops). Location: Department of Biochemistry, Maastricht University, Universiteitssingel 50, Maastricht Multi-parametric analysis of thrombus formation at physiological shear flow – in collaboration with Westburg/Fluxion Biosciences (Timo Kreike) Application of advanced microscopy in studies of in vivo and in vitro thrombus formation – Biochemistry Department, CARIM, Maastricht University (Judith Cosemans, Johan Heemskerk) Platelet function analyses on the BD Accuri C6 cytometer – in collaboration with BD (Paul Harrison) Desktop scanning electron microscopy – in collaboration with Phenom-World (Bas de Laat, Fleur van Wely) 18.00 - Registration with bits and bites Location: Amrâth Grand Hôtel de l’Empereur, Maastricht 19.00 - 21.00 Special clinical session: Platelets, bleeding and pro-hemostatics (open to all participants). Location: Amrâth Grand Hotel de l’Empereur, Maastricht Chairs: Johan Heemskerk and Judith Cosemans 19.00 Stroke still needs to be prevented: does anticoagulation beat antihypertension? Thomas Unger (Maastricht, The Netherlands) 19.25 Two-step tests to measure platelet function and von Willebrand factor in whole blood Mark Roest (Utrecht, The Netherlands) 19.50 How to implement flow cytometry for platelet function analysis in the clinic? Paul Harrison (Oxford, United Kingdom) 20.05 Omics approaches to expand our genotype-phenotype knowledge of inherited platelet disorders Kathleen Freson (Leuven, Belgium) 20.30 When does a defect in platelet function give rise to bleeding? Steve Watson (Birmingham, United Kingdom) 21.00 Welcome drink 5 Thursday, 20th September 2012 08.50 - 09.00 Welcome: Johan Heemskerk, Judith Cosemans 09.00 - 10.45 Session I: Clinical aspects Chairs: Christian Gachet and Peter Presek 09.00 Key note lecture: Antiplatelet drugs in patients (non)invasive cardiac interventions Christian Hackeng (Nieuwegein, The Netherlands) 09.30 O1. Lentiviral gene rescue in a Bernard-Soulier mouse model provides a tool to assess the function of GPIb in vivo undergoing Catherine Strassel, Anne Bull, Sylvie Moog, Stephanie Magnenat, Marie Jeanne Baas, Monique Freund, Christian Gachet, François Lanza (Strasbourg, France) 09.45 O2. Only severe thrombocytopenia insufficient thrombus formation in mice results in bleeding and Martina Morowski, Timo Vögtle, Peter Kraft, Guido Stoll, Bernhard Nieswandt (Würzburg, Germany) 10.00 O3. Platelet phenotype and thrombosis in JAK2V617F mice Lamia Lamrani, Catherine Lacout, Véronique Ollivier, Benoit Ho Tin Hoe, William Vainchenker, Jean Luc Villeval, Martine Jandrot-Perrus (Paris, Villejuif, France) 10.15 O4. Platelet Hyperreactivity Project: a network-based approach to elucidate determinants of platelet reactivity in aspirin-treated cardiovascular patients A. Zufferey, M. Ibberson, J.-L. Reny, S. Nolli, M. Docquier, I. Xenarios, J.C. Sanchez, P. Fontana (Geneva, Lausanne, Switzerland) 10.30 O5. Prospective evaluation of platelet activation markers in patients treated with drug-eluting stents: platelet-associated tissue factor predicts the risk of future revascularization Marina Camera, Marta Brambilla, Daniela Trabattoni, Fabrizio Veglia, Paola Canzano, Laura Rossetti, Antonio Bartorelli, Elena Tremoli (Milano, Italy) 10.45 - 11.15 Coffee break 11.15 - 12.45 Session II: Platelet development Chairs – Marc Hoylaerts and Bernard Payrastre 11.15 Key note lecture: RUNX1 and megakaryopoiesis William Vainchenker (Villejuif, France) 11.45 O6. Megakaryocyte-specific deletion of the protein-tyrosine phosphatases Shp1 and Shp2 causes a block in megakaryocyte development and severe macrothrombocytopenia Alexandra Mazharian, Jun Mori, Ying-Jie Wang, Silke Heising, Benjamin G. Neel, Steve P. Watson and Yotis A. Senis (Birmingham, Oxford, United Kingdom; Toronto, Canada) 12.00 O7. BAMBI (BMP and activin membrane bound inhibitor): a novel regulator of platelet production and function Isabelle I. Salles, David A. Lane, James T.B. Crawley (London, United Kingdom) 6 12.15 O8. Role of p110beta and alpha isoforms of class I PI3-kinases in platelet activation and functions Sonia Séverin, Pierre-Alexandre Laurent, Gaëtan Chicanne, Béatrice Hechler, Christian Gachet, Julie Guillermet-Guibert, Bart Vanhaesebroeck, Bernard Payrastre, Marie-Pierre Gratacap (Toulouse, France) 12.30 O9. Maps of open chromatin highlight cell type-specific patterns of regulatory sequence variation at haematological trait loci Augusto Rendon, Dirk S. Paul, Cornelis A. Albers, Katrin Voss, Jonathan Stephens, HaemGen Consortium, Pim van der Harst, John C. Chambers, Nicole Soranzo, Willem H. Ouwehand, Panos Deloukas (Cambridge, Hinxton, London, Middlesex, United Kingdom; Groningen, The Netherlands) 12.45 - 14.15 Lunch 14.15 - 15.45 Session III: Thrombosis and thrombus formation Chairs – Elisabeth Gardiner and Bernhard Nieswandt 14.15 Key note lecture: P2Y12 inhibitors: not only P2Y12, not only platelets, not only thrombosis Marco Cattaneo (Milano, Italy) 14.45 O10. Gap junctions and haemostasis and thrombosis connexin hemichannels underpin Sakthivel Vaiyapuri, Chris I. Jones, Parvathy Sasikumar, Leonardo A. Moraes, Marfoua S. Ali, Tanya Sage, Alexander M. Simon, Martyn P. Mahaut-Smith, Jonathan M. Gibbins (Reading, Leicester, United Kingdom; Tucson, USA) 15.00 O11. Major role for integrin 61 in platelet adhesion and aggregation to an injured arterial wall Mathieu Schaff, ChaoJun Tang, Eric Maurer, Catherine Bourdon, Nicolas Receveur, Béatrice Hechler, Adèle De Arcangelis, Elisabeth Georges-Labouesse, Christian Gachet, François Lanza, Pierre H. Mangin (Illkirch-Graffenstaden, Strasbourg, France) 15.15 O12. P2Y12 synergistically controls a Munc13-4-independent pathway to secretion of α-granules but not dense granules in platelets Matthew T. Harper, Joshua S. Savage, Christopher M. Williams, Alastair W. Poole (Bristol, United Kingdom) 15.30 O13. Grb2 is essential for GPVI-mediated platelet activation S. Dütting, T. Vögtle, M. Morowski, P. Kraft, G. Stoll, L. Nitschke, B. Nieswandt (Würzburg, Erlangen, Germany) 15.45 - 17.15 Poster presentation session A 18.00 – 24.15 Social and culinary program: Maastricht event! Walking tour with dinner starts from Amrâth Grand Hôtel de l’Empereur. A spectacular location is visited in the end. Back at walking distance from the hotel around 24 h. Earlier return is possible refreshments included 7 Friday, 21th September 2012 08.45 - 10.40 Session IV: Platelets in coagulation, inflammation & reproduction Chairs – Hans Deckmyn and Tomas Lindahl 08.45 Key note lecture: Defining the role of platelets during reproduction Berend Isermann (Heidelberg, Germany) 09.15 O14. Targeting factor XII inhibits the pathological process of thrombus formation on ruptured plaques in vivo and in vitro Marijke J.E. Kuijpers, Marion A.H. Feijge, Paola E.J. van der Meijden, Thomas Renné, Johan W.M. Heemskerk, Judith M.E.M. Cosemans (Maastricht, The Netherlands; Stockholm, Sweden) 09.30 O15. Platelets directly enhance transmigration of neutrophils in response to oxidized LDL Sigrun Badrnya, Lynn M. Butler, Cecilia Söderberg-Naucler, Ivo Volf, Alice Assinger (Stockholm, Sweden; Vienna, Austria) 09.45 O16. Coagulation factor XII is not activated by platelets but facilitates platelet-driven propagation of coagulation Lars Faxälv, Sofia Ramström, Kristina Soutukorva, Pentti Tengval, Tomas L. Lindahl (Linköping, Gothenburg, Sweden) 10.10 O17. Neutrophils initiate coagulation on the injured vessel wall Roxane Darbousset, Grace M. Thomas, Soraya Mezouar, Nigel Mackman, Thomas Renné, Françoise Dignat-George, Christophe Dubois, Laurence Panicot-Dubois (Marseille, France; Boston, Chapel Hill, USA; Stockholm, Sweden) 10.25 - 12.00 Poster presentation session B (coffee) 12.00 - 13.20 Lunch 13.20 - 15.00 Session V: Platelet signaling Chairs – Ingeborg Hers and Khalid Naseem 13.20 Key note lecture: Cyclic nucleotides, kinases and signaling in platelets Albert Smolenski (Dublin, Ireland) 13.50 O18. A point mutation in CalDAG-GEFI gene affects inside-out signaling and platelet spreading in humans Matthias Canault, Charlotte Grosdidier, Pierre E. Morange, Marie Guinier, Véronique Baccini, Alan T. Nurden, David Tregouet, Marie-Christine Alessi (Marseille, Paris, France) 14.05 O19. PKCα and Akt -mediated phosphorylation of GSK3 promotes integrin activation and granule secretion in platelets Samantha F. Moore, Marion van den Bosch, Ingeborg Hers (Bristol, United Kingdom) 8 14.20 O20. Oxidized low-density lipoproteins induce platelet activation and shape change through a novel CD36-Rho kinase dependent signalling pathway Katie S. Wraith, Simbarashe Magwenzi, Ahmed Aburima, Yichuan Wen, David Leake, Khalid M. Naseem (Hull, Reading, United Kingdom) 14.35 O21. Dual mechanism of integrin closure in procoagulant platelets Nadine J.A. Mattheij, Karen Gilio, Roger van Kruchten, Shawn M. Jobe, Adam J. Wieschhaus, Athar H. Chishti, Peter W. Collins, Johan W.M. Heemskerk, Judith M.E.M. Cosemans (Maastricht, The Netherlands; Atlanta, Boston, USA; Cardiff, United Kingdom) 14.50 - 15.20 Coffee break 15.20 - 16.50 Session VI: New approaches and directions in platelet research Chairs Mauro Torti and Ivo Volf 15.20 Key note lecture (supported by Netherlands Heart Foundation): Systems biology approaches in platelet research Scott Diamond (Philadelphia, PA, USA) 15.50 O22. G6f-like is an ITAM containing collagen receptor mediating fish haemostasis Craig E. Hughes, Uvaraj P. Radhakrishnan, Stuart Egginton, Johannes M. Dijkstra, Jagadeeswaran Purdur, Steve P. Watson (Birmingham, United Kingdom; Denton, Texas, USA; Toyoake, Aichi, Japan) 16.05 O23. Systematic characterization of human platelets by quantitative proteomics Julia Burkhart, Marc Vaudel, Florian Beck, Stepan Gambaryan, Ulrich Walter, Albert Sickmann, Jörg Geiger, René Zahedi (Dortmund, Würzburg, Mainz, Germany) 16.20 O24. Thrombin stimulation induces de novo SDF-1a synthesis and alters microRNA profile in human platelets Zhangsen Huang, Mohammed Ferdous-Ur Rahman, Lei Jiang, Hong Xie, Stefano Caramuta, Weng-Onn Lui, Nailin Li (Stockholm, Sweden) 16.35 O25. RGS18 integrates activatory and inhibitory signalling in platelets Kristina Gegenbauer, Giuliano Elia, Alfonso Blanco-Fernandez, Albert Smolenski (Dublin, Ireland) 16.50 – 17.00 Farewell and departure 9 POSTER PRESENTATION SESSION A. Thursday 20th September 2012 All abstract scored by the international scientific advisory committee P1 Time- and cost-effective method for monitoring platelet function using a new standardized automated high-throughput test system based on light transmission aggregometry F. Prüller, S. Klinger, F. Fruhwald, S. Macher, A.C. Haushofer, R.B. Raggam, M. Truschnig, E. Mahla (St. Pölten, Graz, Austria) P2 The platelet tetraspanin Tspan9 is required to fine tune GPVI signalling Elizabeth J. Haining, Jing Yang, Marie Lordkipanidzé, Brenda A. Finney, Craig E. Hughes, Steve P. Watson, Michael G. Tomlinson (Birmingham, United Kingdom) P3 P2Y12 signalling is critical for sustained activation of Rap1b in human platelets Hariharan Subramanian, Ulrich Walter, Stepan Gambaryan (Würzburg, Germany) P4 Six-biomarker algorithms identify Alzheimer's disease at high accuracy Maria Zellner, Alexandra Graf, Michael Veitinger, Rita Babeluk, John Lamont, Ellen Umlauf (Vienna, Austria; Crumlin, United Kingdom) P5 Platelet effects on neutrophils: the yin and the yang Benoît Ho-Tin-Noé, Lamia Lamrani, Véronique Ollivier, Stéphane Loyau, Martine Jandrot-Perrus (Paris, France) P6 Alterations of platelet hemostatic markers during storage of platelet concentrates Alfonso Vignoli, Marina Marchetti, Carmen Julia Tartari, Laura Russo, Erika Diani, Cinzia Giaccherini, Cristina Verzeroli, Luca Da Prada, Stefana Marziali, Anna Falanga (Bergamo, Italy) P7 The hydrogen sulfide releasing aspirin derivative ACS14 exerts strong cyclooxygenase independent antiplatelet effects Joachim Pircher, Franziska Fochler, Thomas Czermak, Hanna Mannell, Piero Del Soldato, Ulrich Pohl, Florian Krötz (Munich, Germany; Milano, Italy) P8 Regulation of platelet GPCR receptor function by NHERF protein isoforms in mouse platelets Robert J.P. Pope, Shaista P. Nisar, Margaret R. Cunningham, Alastair W. Poole, Stuart J. Mundell (Bristol, United Kingdom) P9 Human circulating microparticles: a global assessment of their physical and antigenic proprieties by a multicolor flow cytometry approach Daniela Boselli, Marta Brambilla, Daniele Manganaro, Elena Tremoli, Marina Camera. (Milano, Italy) P10 Redundant functions of phospholipases D1 and D2 in platelet -granule release Ina Thielmann, David Stegner, Peter Kraft, Ina Hagedorn, Christoph Kleinschnitz, Guido Stoll, Bernhard Nieswandt (Würzburg, Germany) P11 Inverse role of pulsatile blood flow on thrombus growth under physiological and disturbed rheological conditions Eric Maurer, Christian Gachet, François Lanza, Pierre Mangin (Strasbourg, France) 10 P12 Analysis of hemostatic properties of blood platelets after treatment with different forms of homocysteine in vitro Joanna Malinowska and Beata Olas (Lodz, Poland) P13 Synaptotagmin-like protein 4 regulates platelet granule secretion Ashling Hampson, Albert Smolenski (Dublin, Ireland) P14 High on-treatment platelet reactivity (HPR) during clopidogrel treatment in minor stroke or TIA: impact of glucose metabolism and insulin resistance Annika Lundström, Ann-Charlotte Laska, Magnus von Arbin, Håkan Wallén (Stockholm, Sweden) P15 Multiparameter assessment of thrombus formation: a systems biology approach Susanne M. de Witt, Moniek M.E. Lamers, Frauke Swieringa, Roger van Kruchten, Richard W. Farndale, Kenneth J. Clemetson, Judith M.E.M. Cosemans, Johan W.M. Heemskerk (Maastricht, The Netherlands; Cambridge, United Kingdom; Bern, Switzerland) P16 In vivo efficacy of platelets produced by megakaryocyte exposure to high shear rates Sonia Poirault-Chassac, Audrey Pietrzyk, Evelyne Lauret, Cécile V. Denis, Dominique Baruch (Paris, Le Kremlin-Bicêtre, France) P17 Romiplostim administration shows reduced megakaryocyte response capacity and increased myelofibrosis in a mouse model of MYH9-RD Catherine Léon, Katja Evert, Frank Dombrowski, Fabien Pertuy, Anita Eckly, Patricia Laeuffer, Christian Gachet, Andreas Greinacher (Strasbourg, France) P18 Evaluation of a new flow cytometric bead assay for VASP-phosphorylation to assess the effectiveness of P2Y12 antagonists in patients with acute coronary syndrome and its comparison with the Biocytex VASP test Natalia Dovlatova, Jacqueline Glenn, Ann White, Stan Heptinstall, Susan Fox (Nottingham, United Kingdom) P19 Dimers of the platelet collagen receptor GPVI are essential for platelet adhesion and activation Stephanie M. Jung, Masaaki Moroi, Nicholas Pugh, Steve P. Watson, Richard W. Farndale (Birmingham, Cambridge, United Kingdom) P20 Misfolded extracellular proteins activate plateletsby interaction with the stress response cell surface chaperones, glucose-regulated protein (GRP) 78/BIP, and thiol isomerases Anne Bertling, Kerstin Jurk, Martin F. Brodde, Christopher Stavermann, Meike Horn, Muzaffar Hussain, Christina Stürzel, Anja Müller, Jerzy-Roch Nofer, Judith Lahav, Hugo Van Aken, Christine Heilmann, Beate E. Kehrel (Münster, Germany; Petah Tikva, Israel) P21 Functional and morphological characterization of the three activated platelet subpopulations Sergey I. Obydennyi, Nadezhda A. Podoplelova, Faina Y. Verholomova, Elena O. Artemenko, Natalia V. Zakharova, Mikhail A. Panteleev (Moscow, Russia) P22 Characterization of novel tyrosine kinase pathways regulating platelet functional responses Todd Getz, Lorena Butriago, Yingying Mao, Satya P. Kunapuli (Philadelphia, USA) 11 P23 In human platelets the multidrug resistance protein 4 (MRP4) is induced by aspirin Isabella Massimi, Raffaella Guerriero, Lavinia Vittoria Lotti, Valentina Lulli, Federico Romani, Luigi Frati, Fabio M. Pulcinelli (Rome, Italy) P24 N-octanoyl-dopamine is a potent inhibitor of platelet function Angelika Schedel, Lamia Ait-Hsiko, Tineke Kraaij, Bastian Theisinger, Benito Yard, Peter Bugert (Baden-Württemberg, Mannheim, Heidelberg, Germany) P25 Comprehensive analysis of the CLEC-2 signaling cascade by platelet proteomics: novel clues on CLEC-2 signaling regulation Andrés F. Parguiña, Isaac Rosa, Johannes A. Eble, Ángel García (Santiago de Compostela, Spain; Frankfurt am Main, Germany) P26 Development and characterization of a dimeric peptidomimetic of platelet GPVI Peng Jiang, Jérome Hérault, Faisal Alshoukr, Rana Benazzouna, Laure Sarda-Mantel, Chang-Zhi Dong, Martine Jandrot-Perrus (Paris, France) P27 Low biological variation protein characterization by 2D DIGE as new putative normalization standards R. Baumgartner, M. Veitinger, E. Umlauf, S. Guterres, I.Volf, J. Lamont, R. Öhler, M. Zellner (Crumlin, United Kingdom; Vienna, Austria) P28 Oxidised LDL activates platelets NADPH oxidase-dependent inhibition of the cGMP/protein kinase G signalling cascade Simbarashe Magwenzi, Catriona McNeil, Katie Wraith, Khalid Naseem (Hull, United Kingdom) P29 Effects of grape seed extract on antiplatelet function of endothelial cells Michał Bijak, Bogusława Luzak, Anna Kosiorek, Kamila Syska, Joanna Saluk, Marek Rożalski, Cezary Watala, Paweł Nowak, Barbara Wachowicz, Jacek Golański (Lodz, Poland) P30 Automated analysis of dynamic in vitro thrombus formation Loic Peter, Sjoert B.G. Jansen, Willem H. Ouwehand, Nassir Navab, Peter A. Smethurst (Cambridge, Hinxton, United Kingdom; Munich, Germany) P31 Desmopressin treatment improves whole-blood thrombus formation in patients undergoing cardiothoracic surgery Frauke Swieringa, Gerhardus J.A.J.M Kuiper, Marcus D. Lancé, Lieke Verheijen, Kevin Hughes, Marco Marcus, Johan W.M. Heemskerk, Paola E.J van der Meijden (Maastricht, The Netherlands) P32 Increased platelet reactivity and platelet-monocyte interaction in HIV: an underestimated cardiovascular risk factor? Quirijn de Mast, Anna Schonsberg, Till Mathan, Meta Michels, Rob Fijnheer, Philip de Groot, André van der Ven (Nijmegen, Utrecht, The Netherlands) P33 Antithrombotic actions of statins involve PECAM-1 signaling Leonardo A Moraes, Chris I. Jones, Sakthivel Vaiyapuri, Parvathy Sasikumar, Neline Kriek, Marfoua Ali, Tanya Sage, Jonathan M Gibbins (Reading, United Kingdom) 12 P34 Predictive value of thromboelastography for 1-year outcome after ST-elevated myocardial infarction Thea C. Godschalk, Thomas O. Bergmeijer, Arnoud Löwik, Jurriën M. ten Berg, Chris M. Hackeng (Nieuwegein, The Netherlands) P35 Quantitative, sensitive and robust determination of ATP and ADP secretion from human and mouse platelets by an HPLC assay Jörg Geiger, Claudia Schütz, Stepan Gambaryan (Würzburg, Germany) P36 P-selectin tests to monitor platelet function remotely in cardiac patients Sue Fox, Yanushi D Wijeyeratne, Mark Thomas, Andrew Johnson, Jane May, Stan Heptinstall (Nottingham, United Kingdom) P37 Absence of role for myosin Va in regulating platelet function Matthew T. Harper, Marion T.J. van den Bosch, Ingeborg Hers, Alastair W. Poole (Bristol, United Kingdom) P38 Dual regulation of CD84 by ADAM10 and calpain in platelets Sebastian Hofmann, Timo Vögtle, Markus Bender, Stefan Rose-John, Bernhard Nieswandt (Würzburg, Kiel, Germany) P39 Calcium-dependent cyclic nucleotide regulation by two PDE1 isoforms in platelets Cathrin Horras, Sabine Gätzner, Sabine Herterich, Jörg Geiger (Würzburg, Germany) P40 Chronic estradiol treatment reduces platelet responses and protects mice from thromboembolism through the haematopoietic estrogen receptor Marie-Cécile Valéra, Marie-Pierre Gratacap, Pierre Gourdy, Françoise Lenfant, Cendrine Cabou, Celine E. Toutain, Marlene Marcellin, Nathalie Saint Laurent, Pierre Sié, Michel Sixou, Jean-François Arnal, Bernard Payrastre (Toulouse, France) P41 Pollution derived diesel exhaust nanoparticles and their effects on human platelets Antonia Solomon, Erica Smyth, Naeem Mitha, Simon Pitchford, Pradeep Luther, Andrew Thorley, Terry Tetley, Michael Emerson (London, United Kingdom) P42 A novel flow cytometry-based platelet aggregation assay Iris M. De Cuyper, Marjolein Meinders, Edith van de Vijver, Dirk de Korte, Leendert Porcelijn, Masja de Haas, Johannes A. Eble, Taco W. Kuijpers (Amsterdam, The Netherlands; Frankfurt am Main, Germany) P43 High plasma levels of prostaglandin E2 in cord blood affect newborn platelet function Axel Schlagenhauf, Hans-Jörg Leis, Bettina Leschnik, Wolfgang Muntean (Graz, Austria) P44 Investigating the mechanism of action of sildenafil on platelet function in vitro and in vivo Georgina Apostoli, Michael Emerson (London, United Kingdom) P45 Mechanism and target of action of Phikud Navakot extracts on human platelet P. Pradmeeteekul, W. Kaewprem, N.P. Morales, U. Sotanaphun, D. Meksuriyen, P. Rojnuckarin, R. Luechapudiporn (Chulalongkorn, Mahidol, Nakhon Pathom, Thailand) 13 P46 Lower levels of coated-platelets can be increased by hydroxyurea treatment in essential thrombocytopenia Béla Nagy Jr, Gyula Reményi, Róbert Szász, Ildikó Beke Debreceni (Debrecen, Hungary) P47 Immobilized amyloid peptide supports platelet adhesion Ilaria Canobbio, Laura G. Di Pasqua, Silvia Catricalà, Cesare Balduini, Mauro Torti (Pavia, Italy) P48 Whole blood thrombin generation monitored with a calibrated automated thrombogram-based assay Marisa Ninivaggi, Rafael Apitz-Castro, Yesim Dargaud, Bas de Laat, H. Coenraad Hemker, Theo Lindhout (Maastricht, The Netherlands; Lyon, France) 14 POSTER PRESENTATION SESSION B. Friday, 21th September 2012 P50 Platelet adhesive functions are differentially affected in patients with filamin A mutations Eliane Berrou, Frédéric Adam, Marilyne Lebret, Patricia Fergelot, Alexandre Kauskot, Martine Jandrot-Perrus, Alan Nurden, Rémi Favier, Jean-Philippe Rosa, Cyril Goizet, Paquita Nurden, Marijke Bryckaert (Kremlin Bicêtre, Paris, Bordeaux, Pessac, Villejuif, France) P51 Changes in platelet function during storage: evaluation of a flow cytometry method for studies of platelet adhesion and activation Anna Södergren, Nahreen Tynngård, Sofia Ramström (Linköping, Sweden) P52 A novel real-time whole blood flow cytometric assay identifies a subpopulation of platelets that rapidly bind annexin-V upon stimulation Chris I. Jones, Stephen F. Garner, Jonathan M. Gibbins (Reading, Cambridge, United Kingdom) P53 Beta2-glycoprotein I has a protective function in situations of hyper-active von Willebrand factor: implications for TTP Vivian X. Du, Gwen van Os, Johanna A. Kremer Hovinga, Ilze Dienava-Verdoold, Jacques Wollersheim, Zaverio M. Ruggeri, Rob Fijnheer, Philip G de Groot, Bas de Laat (Maastricht, Utrecht, Amsterdam, The Netherlands; La Jolla, USA) P54 A role for histone deacetylases in the regulation of platelet function Marfoua S. Ali, Sakthivel Vaiyapuri, Chris I. Jones, Jonathan M. Gibbins, Michael J. Fry (Reading, Cambridge, United Kingdom) P55 The potential antithrombotic properties of polyphenolic-polysaccharides conjugates from plants of Asteraceae and Rosaceae families Joanna Saluk, Michał Bijak, Izabela Pawlaczyk, Paweł Nowak, Roman Gancarz, Barbara Wachowicz (Lodz, Wroclaw, Poland) P56 Continuous platelet production in the bloodstream is regulated by von Willebrand factor Sonia Poirault-Chassac, Kim Anh Nguyen, Audrey Pietrzyk, Caterina Casari, Agnès Veyradier, Cécile V. Denis, Dominique Baruch (Paris, Le Kremlin-Bicêtre, Clamart, France) P57 Defining the effects of latex nanoparticles and their physicochemical properties on platelet function to assess the thrombotic risk from ambient particulate matter E. Smyth, A. Solomon, A. Thorley, T. Tetley, M. Emerson (London, United Kingdom) P58 Role of AMPK signalling in human platelet aggregation Marie-Blanche Onselaer, Stéphane Eeckhoudt, Luc Bertrand, Jean-Louis Vanoverschelde, Cécile Oury, Christophe Beauloye, Sandrine Horman (Liège, Brussels, Belgium) P59 CLP36 is a negative regulator of GPVI signaling in platelets Shuchi Gupta, Attila Braun, Martina Morowski, Thomas Premsler, Markus Bender, Zoltan Nagy, Albert Sickmann, Heike Hermanns, Michael Bösl, Bernhard Nieswandt (Würzburg, Dortmund, Martinsried, Germany) 15 P60 Effect of storage temperature on in vitro quality of platelet concentrates Johan W Lagerberg, Lara A. de Laleijne-Liefting, Pieter F van der Meer, Dirk de Korte (Amsterdam, The Netherlands) P61 Glucose prevents inhibition of platelets caused by NOS activation Gennadi Kobzar, Vilja Mardla, Nigulas Samel (Tallinn, Estonia) P62 The JAK2V617F mutation causes an increase in platelet reactivity in a knock-in mouse model of essential thrombocythaemia Catherine M. Hobbs, Cedric Ghevaert, Sonia Severin, Jocelyn Auger, Alexandra Mazharian, Cavan Bennett, Harriet Manning, Lauren Brain, Juan Li, Anthony R. Green, Steve P. Watson (Cambridge, Birmingham, United Kingdom) P63 In vivo models for evaluating mouse and human platelet aggregation responses Charalambos Tymvios, Lisa-Marie Holbrook, Christopher Moore, Antonia Solomon, Michael Emerson (London, United Kingdom) P64 Diving into murine fetal and adult megakaryopoiesis M. Meinders, I.M. De Cuyper, T.K. van den Berg, L. Gutiérrez (Amsterdam, The Netherlands) P65 Thrombopoiesis is not impaired in dengue-induced thrombocytopenia Amaylia Oehadian, Meta Michels, Pandji Irani Fianza, Quirijn de Mast, Rudi Wisaksana, Bachti Alisjahbana, André van der Ven (Bandung, Indonesia; Nijmegen, The Netherlands) P66 The role of Eph-Ephrin signaling in platelet function Rekha H. Rana, Tanya Sage, Parvathy Sasikumar, Sakthivel Vaiypapuri, Neline Kriek, Jonathan M. Gibbins (Reading, United Kingdom) P67 Paradoxical up-regulation of platelet activation by anti-platelet therapies as a consequence of a disruption of P2Y-TP receptor cross-talk M.I. Butler, R.J.P. Pope, D.A. Marsden, J.F. Barton, A.D. Mumford, A.W. Poole, T.W. Johnson, S.J. Mundell (Bristol, United Kingdom) P68 Effect of three-dimensional hydrogel scaffolds on megakaryocyte differentiation Audrey Pietrzyk, Sonia Poirault-Chassac, Didier Letourneur, Catherine Le Visage, Dominique Baruch (Paris, France) P69 LASP-1 deficiency in mice leads to increased bleeding time and impaired platelet activation Cora Reiss, Stepan Gambaryan, Ulrich Walter, Elke Butt (Würzburg, Germany) P70 Expression of homopentameric and heteropentameric acetylcholine receptors in megakaryocytes derived from CD34+ progenitor cells Angelika Schedel, Kerstin Kaiser, Steffi Uhlig, Florian Lorenz, Karen Bieback, Peter Bugert (Heidelberg, Mannheim, Germany) P71 Favourable modulation of platelet function in female smokers by 12 weeks of aerobic endurance training Werner Schmid, Alice Assinger, Corinna Schlögl, Ivo Volf (Vienna, Austria) P72 Rhinocetin, a venom-derived integrin-specific antagonist inhibits collagen induced platelet and endothelial cell functions 16 Sakthivel Vaiyapuri, Marfoua S. Ali, Robert A. Harrison, Andrew B. Bicknell, Jonathan M. Gibbins (Liverpool, Reading, United Kingdom) P73 Genetic deletion of Vaccinia H1-related (VHR) phosphatase inhibits collageninduced platelet activation and thrombus formation without affecting bleeding time in mice Lucia Musumeci, Lisbeth Maurissen, Marijke J. Kuijpers, Fabio Cerignoli, Christelle Lecut, William Guilmain, Marc Thiry, Yotis Senis, Michel Moutschen, Tomas Mustelin, Lutz Tautz, Johan W.M. Heemskerk, Cécile Oury, Souad Rahmouni (Liège, Belgium; Maastricht, The Netherlands; La Jolla, USA; Birmingham, United Kingdom) P74 Activation of PAR4 by - and -thrombin is critically dependent on anion binding exosite II Niklas Boknäs, Maria Wallstedt, Lars Faxälv, Tomas Lindahl (Linköping, Sweden) P75 Scott syndrome platelets demonstrate presence of TMEM16F-dependent and independent phospholipid scrambling in both platelet apoptosis and platelet activation Roger van Kruchten, Nadine J.A. Mattheij, Christine Saunders, Marion A.H. Feijge, Peter W. Collins, Johan W.M. Heemskerk, Edouard M. Bevers (Maastricht, The Netherlands; Cardiff, United Kingdom) P76 Platelet flow-induced protrusion (FLIPR) formation leading to platelet disintegration and procoagulant microparticles C. Tersteeg, H.F. Heijnen, G. Pasterkamp, R.T. Urbanus, C. Maas, I. Hoefer, E.M. Mak-Nienhuis, R. Nieuwland, F. Storti, F.N. van de Vosse, Ph.G. de Groot, M. Roest (Utrecht, Amsterdam, Eindhoven, The Netherlands) P77 Novel SNARE family members contribute to regulation of platelet granule secretion Ewelina M. Golebiewska, Robert A.N. Goggs, Christiane Wiegand, Gabriele Fischer von Mollard, Alastair W. Poole (Bristol, United Kingdom; Bielefeld, Germany) P78 Regulation of the guanine nucleotide exchange factor CalDAG-GEFI by protein kinase A Gianni Guidetti, Alessandra Consonni, Daria Manganaro, Ilaria Canobbio, Cesare Balduini, Mauro Torti (Pavia, Italy) P79 Platelet-associated tissue factor expression: shedding light on a controversial issue Laura Facchinetti, Daniela Boselli, Marta Brambilla, Paola Canzano, Elena Tremoli, Marina Camera (Milano, Italy) P80 Periodontopathogens induce expression of CD40L on human platelets via TLR2 and TLR4 Alice Assinger, Markus Laky, Sigrun Badrnya, Azadeh Esfandeyari, Ivo Volf (Vienna, Austria; Stockholm, Sweden) P81 Antiplatelet and antioxidative action of Trifolium pallidum and Trifolium scabrum extracts J. Kolodziejczyk-Czepas, B. Olas, J. Malinowska, B. Wachowicz, B. Szajwaj, I. Kowalska, W. Oleszek, A. Stochmal (Lodz, Pulawy, Poland) 17 P82 Leukocyte-associated immunoglobulin-like receptor-1 deletion in megakaryocytes causes mild thombocytosis and formation of hyperactive platelets Alexandra Mazharian, Steven G. Thomas, Marie Lordkipanidze, Linde Meyaard, Yotis A. Senis, Steve P. Watson (Birmingham, United Kingdom; Utrecht, The Netherlands) P83 Murine platelet responsiveness is highly determined by its hyperlipidemic environment Marco van der Stoep, Menno Hoekstra, Ronald J. van der Sluis, Miranda van Eck, Suzanne J.A. Korporaal (Leiden, Utrecht, Amsterdam, The Netherlands) P84 Myosin IIA deficiency impairs granule distribution and actin dynamics in megakaryocytes and platelets Fabien Pertuy, Anita Eckly, Josiane Weber, François Lanza, Christian Gachet, Catherine Léon (Strasbourg, France) P85 Large-scale screening for extracellular protein interactions involved in platelet aggregation Yi Sun, Gavin J. Wright (Hinxton, United Kingdom) P86 One hour resting effect on day 1 could be mimicked by 6 hours resting on 48 hours stored platelet concentrates by interruption of agitation at room temperature in permeable bags N.H. Timori, F.A. Soleimany, S.M.R. Tabatabai, Sh. Vaeli, B.A. Badlou (Zeist, The Netherlands, Teheran, Iran) P87 Endocannabinoids modulates the number of megakaryocytes in the bone marrow Patrizia Portanova, Sara Sampietro, Michela Nalin, Alessandra Bagarotti, Fabiola Sinigaglia, Alessandra Bertoni (Novara, Italy) P88 Identification of components of the blood coagulum according to SCHULTE inducing proliferation of osteoblast-like cells S. Krause, C. Linß, K. Vogel, J. Schubert, P. Presek (Halle-Wittenberg, Germany) P89 Effects of cyclic nucleotides on CLEC-2-dependent platelet Alessandra Borgognone, Steve P. Watson (Birmingham, United Kingdom) P90 Would a new focus on vascular prostaglandins improve antiplatelet treatment regimes? Stan Heptinstall, David Iyú, Jacqueline R. Glenn, Ann E. White, Sue Fox (Nottingham, United Kingdom) P91 Targeting of type I protein kinase A to lipid rafts is required for competent platelet inhibition by the cAMP-signaling pathway Zaher Raslan, Ahmed Aburima, Simbareshe Magwenzi, Kjetil Tasken, Khalid M Naseem (Hull, United Kingdom; Oslo, Norway) P92 Deficiency of Src homology 2 domain-containing inositol 5-phosphatase 1 (SHIP1) affects membrane cytoskeleton organization and platelet internal contraction Sonia Séverin, Alessandra Consonni, Gaëtan Chicanne, Sophie Allart, Bernard Payrastre, Marie-Pierre Gratacap (Toulouse, France; Pavia, Italy) 18 activation P93 cAMP signaling pathway regulates platelet shape change through modulation of RhoA activation and Rho Kinase-dependent signalling Ahmed Aburima, Katie Wraith, Khalid. M. Naseem (Hull, United Kingdom) P94 Shear-induced release of platelet receptors by metalloproteinases Elizabeth Gardiner (Melbourne, Australia) P95 Influence of the paraoxonase-1 Q192R genetic variant on clopidogrel responsiveness and recurrent cardiovascular events: a systematic review and meta-analysis J.-L. Reny, C. Combescure, Y Daali, P. Fontana, for the PON1 meta-analysis group (Geneva, Switzerland) P96 The contribution of plasmatic proteins and SNPs to high biological variation in the platelet proteome: a combined proteomic and genetic analysis Michael Veitinger, Ellen Umlauf, Roland Baumgartner, Sigrun Badrnya, Christopher Gerner, John Lamont, Rudolf Oehler, Maria Zellner (Crumlin, United Kingdom; Vienna, Austria) P97 Hypercholesterolemia is not necessarily associated with hyperreactive platelets Suzanne J.A. Korporaal, Valentina de Angelis, Menno Hoekstra, Andrea Bochem, Barbara Sjouke, Ronald J. van der Sluis, G. Kees Hovingh, Mark Roest, Miranda van Eck (Leiden, Utrecht, Amsterdam, The Netherlands) P98 Subtle role of platelet thrombospondin-1 interaction with CD36 in murine thrombus formation on collagen Marijke J.E. Kuijpers, Reyhan Nergiz-Unal, Susanne de Witt, Roger van Kruchten, Suzanne J.A. Korporaal, Maria Febbraio, Marc F. Hoylaerts, Marc Tjwa, Peter J. Voshol, Judith M.E.M. Cosemans, Johan W.M. Heemskerk (Maastricht, Leiden, The Netherlands; Leuven, Belgium; Cleveland, USA; Frankfurt, Germany; Cambridge, United Kingdom) 19