The laboratory colony of Phlebotomus perniciosus (Diptera
Transcrição
The laboratory colony of Phlebotomus perniciosus (Diptera
The laboratory colony of Phlebotomus perniciosus (Diptera, Psychodidae) from Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal: establishment, maintenance and application. Rocha1, D.K., Alves-Pires2, C., Gomes3, J., Bruno de Sousa4, C., Branco1, S., Afonso1, M.O. 1. Unidade de Ensino e Investigação de Parasitologia Médica (UEI PM), Grupo Entomologia Médica, Unidade de Parasitologia e Microbiologia Médicas (UPMM/ FCT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa (UNL), Rua da Junqueira, 100, 1349-008, Lisboa, Portugal; 2. UPMM/FCT, IHMT, UNL; 3. CMDT, IHMT, UNL; 4. Universidade do Algarve, Portugal. [email protected] There are five phlebotomine species known in Portugal: Phlebotomus papatasi (Scopoli, 1786), P. Sergenti Parrot, 1917, P. perniciosus Newstead, 1911, P. ariasi Tonnoir, 1921 and Sergentomyia minuta (Rondani, 1843). P. perniciosus and P. ariasi are proven vectors of Leishmania infantum, the protozoan of human and canine leishmaniasis. Females of P. perniciosus have been captured infected with L. infantum in Trás-os-Montes and Alto Douro, in Lisbon and Algarve region. To date, this sand fly species is the most widespread vector due to the bioclimatic characteristics. The colony of Phlebotomus perniciosus IHMT / UPMM / UNL was established in mid1990´s, by Researcher and phlebotomine sand fly specialist Carlos Alves-Pires. The specimens were originated from Spain and supplied by the eminent Scientist Professor Dr. Robert Killick-Kendrick. To date, this is the first successful colonization of sand flies for consecutive generations in Portugal. Sand flies require laborious rearing and maintenance laboratory methods, but over the years the colony has been successfully maintained by researchers and technicians who ensure the availability of these insects for teaching and research. The aim of this work is to report the main rearing methods, namely safety, hygiene, physical, environmental and biological control, equipments, maintenance of adults and immature stages, and different types of food. This colony is used in: insecticide-repellent bioassay tests; experimental infections; molecular studies; laboratorial classes; students training; sending of specimens to national and international Teaching and Research Institutions.