Establishment of growth curve of Pfaffia

Establishment of growth curve of Pfaffia glomerata
(Spreng) root culture
SILVA, T.F.O.1*; YAMAGUCHI, C.S.1; SINZKER, R.C.2; OLIVEIRA, A.J.B1,2;
GONÇALVES, R.A.C.1,2 *
1Programa de Pós Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Maringá -PR, Brazil.
2 Departamento de Farmácia, Universidade Estadual de Maringá, Maringá-PR, Brazil
*[email protected]
Keywords: Pfaffia glomerata, root culture, metabolites.
INTRODUCTION
The Pfaffia glomerata (Spreng) Perderson,
(Amaranthaceae) is popularly known as “Brazilian
ginseng”. Their roots have bioactive compounds and
ecdysterone has been described as the principal
1
constituent . The roots that are used in folk medicine
as a tonic, aphrodisiac, and to treat gastric
disturbance and are commercialized in Brazil as
2
substitutes for the Asian ginseng (Panax ginseng) .
Chemical and pharmacological studies of this
species are scarce compared the extent of their use.
The propagation by cuttings was low multiplication,
restricting
its
large-scale
production
for
2
pharmaceutical purposes . The establishment of
growth curve of organs, such as roots, is essential
due to the possible of identification the growth
phases
that
happen
importants
metabolic
3,4
processes . The aim of this study was to determine
the growth curve of P. glomerata root culture in
liquid medium in the rotary shaker.
RESULTS AND DISCUSSION
In order to determine the growth curve of P.
glomerata root cultures, 200mg of root from culture
in vitro in solid MS medium (Murashige and Skoog,
1962) were inoculated into the 20mL of liquid
medium MS and phytoregulator indole butyric acid
-1
(0.3 mg L IBA) previously optimized. After 7 days,
the tissues were weighed to determine the dry
weight (DW). The growth curves were plotted
according to DW of roots biomass from three
replicates flasks that were harvested weekly for up
to 8 weeks. According to Figure 1, we observed that
the time required to obtain the best root growth is
between four and five weeks (five times bigger than
the initial biomass), after this there wasn´t significant
growth, possibly due to beginning of cellular death.
This phase of decline happen reduction of nutrients
3
and accumulation of toxic substances .
Figure 1. Growth curve of Pfaffia glomerata root culture
in liquid medium the according to DW of roots biomass.
CONCLUSION
In conclusion, it was possible to establish the
growth curve of P. glomerata roots culture of in a
-1
rotary shaker with 0,3 mg L IBA, the optimal growth
was achieved is between four and five weeks. This
technical can be used provides a rapid growth of
plant material can be scale-up, in order to obtain
primary and secondary metabolites of interest.
ACKNOWLEDGEMENTS
The authors are grateful to CAPES and PPG.
REFERENCES
1. Moura, C. L. Avaliação da atividade antimicrobiana dos
estratos brutos das espécies vegetais Miconia rubiginosa e
Pfaffia glomerata em microrganismos da cavidade bucal.
Mestrado, Universidade de Franca, Franca,SP, 2006.
2. Flores, R., et. al. Micropropagation and β-ecdysone content of
the Brazilian ginsengs Pfaffia glomerata and Pfaffia tuberosa In
Vitro Cell.Dev.Biol.—Plant , 2010, 46, 210–217.
3. Nogueira, R. C., et. al. Curva de crescimento e análises
bioquímicas de calos de murici-pequeno (Byrsonima intermedia
A. Juss.). Rev. Bras. Pl. Med., Botucatu, 2008, 10, 44-48
4. Saiman, M. Z.; Mustafa, N. R.; Schulte, A. E.; Verpoorte, R.;
Choi, Y. H. Induction, characterization, and NMR-based metabolic
profiling of adventitious root cultures from leaf explants of Gynura
procumbens. Plant Cell Tiss Organ Cult, 2012, 109, 465–47
VII Workshop on Biocatalysis and Biotransformations and 1o Simposio Latinoamericano de Biocatalisis y Biotransformaciones