Activities Reports - INCT

INCTBio
Instituto Nacional de Ciência e Tecnologia de Bioanalítica
I – SCIENTIFIC REPORT
Period: 03/2009 to 04/2010
FAPESP Process: 2008/57805-2
CNPq Process: 573672/2008-3
Coordinator: Lauro Tatsuo Kubota
Headquarter Institution: Institute of Chemistry - Unicamp
Campinas, 2010.
1
RESEARCHERS OF INCTBio
Prof. Dr. Alviclér Magalhães (IQ-Unicamp)
Prof. Dr. Ana Valéria Colnaghi Simionato (IQ-Unicamp)
Prof. Dr. Auro Atsushi Tanaka (UFMA)
Prof. Dr. Carla Beatriz Grespan Bottoli (IQ-Unicamp)
Prof. Dr. César A. Mello (Unifran) fired and excluded
Prof. Dr. César Ricardo Teixeira Tarley (Unifal-MG) moved to UFU
Prof. Dr. Claudimir Lúcio do Lago (IQ-USP)
Prof. Dr. Dosil Pereira de Jesus (IQ-Unicamp)
Prof. Dr. Eduardo Carasek da Rocha (DQ/CFM-UFSC)
Prof. Dr. Emanuel Carrilho (IQSC-USP)
Prof. Dr. Érico Marlon de Moraes Flores (UFSM)
Prof. Dr. Fabio Augusto (IQ-Unicamp)
Prof. Dr. Fábio Cesar Gozzo (IQ-Unicamp)
Prof. Dr. Flávio Santos Damos (UFVJM)
Prof. Dr. Jez Willian Batista Braga (UnB)
Prof. Dr. José Alberto Fracassi (IQ-Unicamp)
Prof. Dr. Lauro Tatsuo Kubota (IQ-Unicamp) - Coordinator
Prof. Dr. Marcelo Martins de Sena (UEG) moved to UFMG
Prof. Dr. Marco Aurélio Zezzi Arruda (IQ-Unicamp)
Prof. Dr. Marco Flores Ferrão (UNISC)
Prof. Dr. Marília de Oliveira Fonseca Goulart (IQB-UFAL)
Prof. Dr. Marina Franco Maggi Tavares (IQ-USP)
Prof. Dr. Orlando Fatibello Filho (UFSCar)
Prof. Dr. Pablo Alejandro Fiorito (UFABC)
Prof. Dr. Pedro de Magalhães Padilha (UNESP)
Prof. Dr. Reinaldo Calixto de Campos (PUC-RJ)
Prof. Dr. Ricardo Erthal Santelli (UFF) moved to UFRJ
Prof. Dr. Ronei Jesus Poppi (IQ-Unicamp)
Prof. Dr. Rose Mary Zumstein Georgetto Naal (FCFRP-USP)
Prof. Dr. Susana Inés Córdoba de Torresi (IQ-USP)
Prof. Dr. Wendel Andrade Alves (UFABC)
Prof. Dr. Yoshitaka Gushikem (IQ-Unicamp) – Vice-coordinator
Prof. Dr. Zeki Naal (FCFRP-USP)
Included:
Prof. Marcello Garcia Trevisan (Unifal MG)
Prof. Wendell Karlos Tomazelli Coltro (UFG)
13 Pos docs.
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REPORT – INCTBIO
The report presented here is related to the activities of Instituto Nacional de Ciência e
Tecnologia de Bioanalítica (INCTBio) developed in the first year into the INCT program. First
of all we need to mention about the difficulties to sign the contract, which took long time to
solve some problems, delaying about 5 months to sign. Additionally, in our case we received
only half of the budget initially applied.
In this first year, meetings (3 in total) of the members of the Managing Committee and
one Workshop with the members of INCTBio were carried out to define the resources
application, assign the tasks and start the project. In these meetings were decided the equipment
to be acquired by importation and in national suppliers, as well as the destination of the
equipment to different universities/groups. The distribution of the scholarships was also decided
by the Managing Committee.
Most of the equipment was acquired and installed during this first year. Some of them
were still in importation process because the CNPq is in slow process. Acquisitions of some
equipment were prioritized to be located in a young groups and universities as an incentive to
nucleate solid groups in Bioanalytical. At the first moment we must to decide how to rearrange
the resources applications due to the received budget was only half to those initially predicted in
the project. This reduction in the budget drastically affected in the equipment acquisitions and as
a consequence the objectives initially proposed.
Some administrative providence were taken such as preparation of the homepage of the
Institute, creation of the logo, task attributions to the members of the institute, way of
communication between the groups, and others. In the first workshop efforts to promote
interactions between researches groups were performed, trying to incentive the participation of
students, exposing the research lines of the members of INCTBio. Some interchange
mechanisms between the groups were established to allow young groups/universities to interact
and collaborate with consolidated groups improving their researches conditions and quality.
These mechanisms allowed students to spend some time in different labs/universities, which is
very important to the education of the students. The number of the undergraduate and graduate
students joined to the INCTBio must be highlighted, showing the contribution of the Institute to
the quantitative growing and efforts to establish Bioanalytical area in the country.
The number of papers and works presented in congress show great productivity of the
INCTBio, some important interactions with the companies are also show the knowledge transfer
to the applied sector. The number of conferences and seminar to disseminate the science and
knowledge produced by the INCTBio shows the importance given to the knowledge transfer.
INCTBio was acting in different segment and researches field as can be seen during the report
that will show some advances and progress in the Bioanalytical area.
Many difficulties were found during this period since the problem with research card
limiting the use and managing the resources, liberation of only a fraction of the resources from
CNPq, difficulties in some importation processes, change the cambio between Brazilian currency
and US Dollar and others. No infrastructure to support the coordination activities like secretary
and others straining the coordinator, making difficult the administrative things. However, some
actions were taken to solve this problem like asking to Unicamp to give more support to
INCTBio.
In general the members of INCTBio were very active in this first year, producing papers,
supervising students, but the most of these activities was developed with the existing
infrastructure because the equipment were acquired and installed recently or are in importation
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process. It will be possible to verify that the number of researchers in the INCTBio growth
quickly, however some left of INCTBio because they already defended their thesis.
In this first year the focus of the INCTBio was to give an incentive and efforts to promote
interactions between the groups/researchers into the institute. The results of these efforts can be
seen in the works carried in collaborations and co-authored papers. The interactions and
cooperation should be expanded in the next years reaching the integration with other INCTs
looking for a multidisciplinary focus to make the Bioanalytical area strong and recognized.
The developed activities into INCTBio will be described in items according to the
subjects presented in the initial proposal to make the understanding easier.
BIOINORGANIC
Comparative metallomics involving bipolar disorder (BD) and transgenic soybean (Glycine
max (L.) Merrill).
This report refers to the comparative metabonomic profiles between blood serum samples
from healthy individuals and patients with bipolar disorder under different treatments, including
lithium or not, and the identification of metabolic differences between these groups. In this way, 1H
NMR spectroscopy was used and, the spectra were generated through chemometrics analysis, using
PCA and PLS-DA. Fifty 50 samples were used and classified into three groups: 25 samples as
control group, which consist of healthy patients, 15 samples of bipolar patients under treatment
using lithium and 10 samples of bipolar patients under treatment using other drugs than lithium.
As partial results, the use of 1H NMR spectroscopy and chemometric analysis proved an
effective strategy to differentiate healthy and bipolar patients as well as to distinguish bipolar
patients under different treatments, using lithium or not. The metabolic displayed large differences
among the groups consisting of glycoprotein lipids, monounsaturated and polyunsaturated lipids,
and molecules related to lipid metabolism (acetate, choline, glutamate and myo-inositol). It is
noteworthy that the method allowed the detection of differential multiple metabolites
simultaneously, providing a general pattern of bipolar affective disorder and its treatment with
lithium.
Additionally, a comparative proteomics study involving transgenic and non-transgenic
soybean seed is under development, in order to detect possible differences in protein expression
between the samples, which may be related to the genetic modification process. In this way, after
applying the seeds protein extraction procedures, they were separated by polyacrylamide gel
electrophoresis (2D-PAGE), using two pH ranges: 3 to 10 and 4 to 7. Then, the gels were scanned
and, using a specific program, the optical densities of protein spots were obtained and used to
evaluate possible changes in protein expressions.
As partial results, 03 protein spots showed variation in their expressions at a 90% level (1.8
times), between pairs of gels with pH gradients of 3-10, and 07 protein spots showed variations in
their expressions for gels with a gradient of pH of 4-7. From those 10 analyzed protein spots, 08
were identified using MALDI-QTOF, and the related protein database. It is also worth mentioning
that 153 proteins from soybeans were already identified. Therefore, the methodology employed in
this work can be an important tool for identifying potential biomarkers for genetically modified
organisms when considering protein expression and image analysis.
Development of analytical methods for assessing the qualitative and quantitative
metallomics of Nile Tilapia (Oreochromis niloticus).
4
In the first stage of the development of research work have been made to collect samples of
plasma, muscle and liver of Nile tilapia and the experiments with the determinations of total protein
present in these samples. A methodology of separations of proteins by 2D PAGE in tissue samples
of muscle and liver was optimized, as well as a study of photo documentation of the images of the
gels.
1.1. Determination of total protein
These determinations were made according to the Bradford method using bovine serum
albumin as standard. The results are listed in Table 1.
Table 1: Concentrations of total protein (concentration ± SD, n = 3) samples of plasma,
muscle and liver of Nile tilapia.
Sample
Total Protein Concentration (g kg-1)
25.7 ± 2.0*
25.1 ± 0.3
5.3 ± 0.3
Plasma
Muscle
Liver
*result expressed in mg mL-1
1.2. Fractionation of proteins in muscle and liver tissue samples by 2D PAGE
The program optimized for the fractionation of proteins from muscle tissue samples using
isoelectric focusing system at first dimension is shown in Table 2.
Table 2: Electrophoretic separation program used in the first dimension (IEF).
Step
Voltage (V)
Accumulation(Vh)
1
500
500
2
1000
800
3
10000
11300
4
10000
3000
The second dimension of electrophoresis was carried out in a 2D-PAGE mode using two
steps, according to Table 3.
Table 3: Electrophoretic separation program used in the second dimension (SDS-PAGE).
Step
Voltage (V)
Electric current (mA)
Power (W)
Time (h)
1
2
90
25
100
0.5
250
25
100
5
The electropherograms showed that the samples of muscle and liver of Nile tilapia has a
high diversity of proteins. In some protein spots, a considerable intensity of the expressed proteins
in the gel can be observed. The average number of spots from muscle samples was 272 ± 16 with a
match of 71.70% and liver samples was 505 ± 12 with a match of 66.40%. Considering the
complexity of the samples, there was a good correlation, and a significant amount of interfering in
the separation procedures, mainly albumin was noted. The good match between the gels, will allow
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the use and optimization of the 2D PAGE methodology for protein separation, as an initial stage of
sample preparation for qualitative metal determination using SR-XFR in each protein spot
obtained. This strategy was predicted to be the second step of the proposed schedule in the project.
Additionally, in the second stage of the work, the conditions for the purification of proteins from
plasma samples of Nile tilapia will be optimized.
Use of chemically imprinted polymers for the development of methods for
preconcentration and/or speciation.
The aims of this project is to explore the concept of chemical imprinting technology,
especially the synthesis of Ion Imprinted Polymers (IIP), for developing methods for on-line
separation/preconcentration, being the espectroanalytical techniques used for metal detection.
Additionally, carbon nanotubes were investigated for the same purpose. Graphite furnace atomic
absorption spectroscopy (GF AAS), flame atomic absorption spectrometry (F AAS) and UV-Vis
spectrophotometry were the techniques employed. In addition to imprinted polymers with ions,
molecularly imprinted polymer (MIP), aiming the determination of phenolic compounds by
amperometry FIA system was also proposed.
Methods for preconcentration were focused in the first year of the project, as provided in the
research plan, and metal ions were determined in water and biological samples (urine) without
prior treatment involving decomposition with mineral acids. The catalytic MIPs were applied to the
monitoring of catecholamines and phenolic compounds in pharmaceutical and environmental
samples.
Use of different methods of extraction and separation of proteins and metallothioneins
induced by cadmium in the sponge Haliclona sp. for environmental applications.
The following activities were held during the first year of this project
• Cultivation of sponge Haliclona sp. in aquarium under controlled conditions;
• Induction of these sponges to Cd2+ at different exposition time;
• Determination of cadmium in water and tissue by ICP OES;
• Use of SDS-PAGE and 2D-PAGE for identification and comparison of extraction
procedures of metallothionein and proteins induced by this element.
In the experiment with cadmium, pieces of 0.5 g of “sponge matrix” were removed, sliced,
and placed on a frame type "clothes line" and inserted into two tanks containing seawater. After
acclimatization, the sponges were exposed to Cd (0, 17, 41, 137 and 326 hours). After each time,
both control and exposed groups were frozen and lyophilized. Ten mg of each lyophilized sample
were removed, totaling 50 mg (pooled) tissue for each group. The tissues were homogenized at the
1:3 (w / v) ratio with extraction buffer (10 mM Tris-HCl pH 9.0, 5 mM β-mercaptoethanol, 0.5 mM
phenyl methyl sulfonyl (PMFS)). Synthesis of a protein of low molecular weight and cysteine-rich
was verified. The induction of MT was observed by SDS-PAGE at periods of 41, 137 and 326
hours of exposure, and the increase was proportional to exposure time.
For MT extraction, after homogenization, the material was centrifuged at 21000g, and each
sample was divided into two aliquots. The first one was submitted to the extraction process by
solvent precipitation, and the second aliquot submitted to the extraction process by
thermocoagulation.
The gel images on UV transilluminator showed higher fluorescence intensity in samples
treated by thermocoagulation than those ones treated using solvent.
In conclusion:
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• The technique SDS-PAGE coupled with derivatization monobromobimano (mBBr)
identified proteins with high content of –SH and low molecular weight, mainly in the range of ~ 25
kDa, characteristic of MTs;
• Using the sample exposed at 326 hours to Cd2+, the extraction thermocoagulation MTs
showed higher recovery than extraction by solvent precipitation (p <0.01) when subjected to SDSPAGE;
• The two-dimensional electrophoresis revealed the induction of different proteins when
compared with the control situation;
• Differences between treatments for the results of two-dimensional electrophoresis were
observed.
Protein-protein interactions studies.
In this reporting period, the technique of cross-linking coupled with mass spectrometry
(MS) in order to increase the detectability of peptides containing cross-linking was explored. These
peptides are the most important species for structural analysis of proteins by MS. The first
approach was to study the fragmentation patterns of peptides to evaluate the pattern of
fragmentation of species. From this study, several patterns of fragmentation, and three markers of
the presence of ions cross-linking were identified. From the analysis of the formation of these ions
markers, a flowchart for the identification of peptides cross-linked (Figure 1) can be established.
O+
N
MS/MS Spectra
(A)
O
m/z 222.1494
222.1494
or 239.1759
No
No DSS
Yes
305.2229
O+
N
No
Dead-end XL
(B)
Yes
NH2 O
m/z 239.1759
147.1134
and 175.1195
O
N
(C)
No
Inter/Intra XL
Yes
+
N
O
m/z 305.2229
H
Inter XL
The potential of these ions markers was explored in experiments of precursor ion scan,
performed on the instrument Synapta HDMS, configuration Q-Tof.ii. For this evaluation, various
protein models were used, as lizozime (Figure 2). These experiments showed that these ions
markers have excellent sensitivity and selectivity, allowing an efficient detection of peptides
containing the cross-linking. Figure 3 shows the identification of these species by the experiment of
scanning ion precursors.
Perspectives:
The techniques developed will be applied in mapping the interaction between the FERM
domain protein focal adhesion kinase (FAK) and myosin protein complex involved in cell signaling
of the muscular system.
7
857
90
%
A
-10
2.50
5.00
7.50
Liso_CON_PIS FLMC-luiz Sm (SG, 2x4)
10.00
12.50
15.00
17.50
20.00
22.50
25.00
27.50
30.00
32.50
35.00
37.50
40.00
42.50
Time
45.00
2: TOF MS ES+
239.176 0.02Da
3.39e3
B
%
90
-10
2.50
5.00
7.50
Liso_CON_PIS FLMC-luiz Sm (SG, 2x4)
10.00
12.50
15.00
17.50
20.00
22.50
25.00
27.50
30.00
32.50
35.00
37.50
Time
42.50
45.00
2: TOF MS ES+
305.223 0.02Da
147
40.00
C
%
90
-10
2.50
5.00
7.50
10.00
12.50
15.00
17.50
20.00
22.50
25.00
27.50
30.00
32.50
35.00
37.50
40.00
Time
45.00
42.50
Figure 2: PIS of lizozime (lower chromatogram) and lizozime reacted with cross-linking reagent
(upper chromatogram for the marker ions of m/z 222 (A) 239 (B) and 305 (C).
Liso_DSS_DDA IL 746 (26.206) Sm (SG, 2x4.00)
1: TOF MS ES+
297
381.7289
100
A
B
%
90
%
382.2330
478.2795
379.2130
762.4453
222.1494
389.7305
4
397.7335
398.2318
356.2638
239.1759
Tim e
14.00
15.00
16.00
17.00
18.00
19.00
20.00
21.00
22.00
23.00
24.00
25.00
479.2830
26.00
763.4632
437.7168
494.2701
355.0822
-10
606.3763
653.3313
979.4808
812.4568
980.4934
0
350
400
450
500
550
600
650
700
750
800
850
900
m /z
1000
950
15.98212624
Liso_DSS_DDA IL 94 (26.302) M3 [Ev60624,It50,En1] (0.100,2,Cm p); Sm (SG, 2x4.00); Cm (94:97)
478.2894
100
y4
4: TO F MSMS 382.74ES+
388
C
OH
15.98212624
Liso_DSS_D DA IL 94 (26.302) M3 [Ev60624,It50,En1] (0.100,2,C m p); Sm (SG , 2x4.00); Cm (94:97)
4: TO F MSMS 382.74ES+
269
D
239.1843
100
b2 b3 b4
KVFGR
y3
y4 y3 y2 y1
379.2209
239.1843
240.1731
%
%
762.4656
240.1731
356.2691
267.2050
267.2050
y1
175.1276
b4
y2
232.1471
763.5027
479.3048
380.2272
215.1235
84.0717
158.0981
268.1767
304.2062
0
50
606.3752
215.1235 222.1658
589.3686
383.1422 461.2599
488.2769
767.5379
711.4582 757.5316
565.3351
651.2969
150
200
250
300
350
400
450
500
550
600
650
700
750
241.1755
223.1669
205.1153
774.1265
m ass
100
0
200
205
210
215
220
225
230
268.1767
247.1526
257.1878
274.1518 281.1366
235
240
245
250
255
260
265
270
275
280
285
301.1760 304.2062
311.2455
290.1416 297.0670
290
295
300
305
310
315
322.2196 325.1525
m ass
320
325
Figure 3: Identification of peptides modified by cross-linking experiments in the scanning ion precursors.
i
Iglesias AH, Santos LFA, Gozzo FC, J. Am. Soc. Mass Spectrom., 2009, (20), 557-566
ii
Iglesias AH, Santos LFA, Gozzo FC, Anal. Chem., 2010 (82), 909-91.
Evaluation of different methods for speciation analysis of Sb(III) and Sb(V) in meglumine
antimoniate.
In Brazil, meglumine antimoniate (Sb linked to NMG) is the drug of first choice in the treatment
of leishmaniasis. However, it is known that the toxicity and action of Sb species present in this
drug are different. Thus, different methods for speciation analysis of Sb(III) and Sb(V) in
meglumine antimoniate were evaluated. The proposed methods involve the separation and
quantification of Sb(III) through the techniques of liquid chromatography coupled to inductively
coupled plasma mass spectrometry with (LC-ICP-MS), liquid chromatography with hydride
generation coupled to inductively coupled plasma mass spectrometry (LC-HG-ICP-MS), flow
8
injection (FI) hydride generation (HG) coupled with atomic absorption spectrometry (AAS) and
flow injection hydride generation coupled to inductively coupled plasma mass spectrometry (FIHG-ICP-MS).
In the initial studies using LC-ICP-MS for speciation of Sb in meglumine antimoniate,
difficulty in selective determination of Sb species present in the sample was observed, mainly due
to the high concentration of Sb(V) compared to Sb(III), where the signals are partially or fully
overlapped in the chromatographic analysis. Thus, investigations on the separation of Sb species
using chromatographic systems and non-chromatographic methods were made, such as coupling of
LC to HG-ICP-MS, which is still in development.
In all cases, detailed assessments were made of the parameters that influence the separation
of Sb species. The conditions employed for liquid chromatography (LC) are described in Table 1
and the chromatograms obtained are shown in Figure 1.
Table 1. Operational parameters for the LC instrument.
Parameters
Condition
Colunm
PRP-X 100, Hamilton
(250 x 4,1 mm ID., 10 µm)
Eluent
EDTA 1,0 mmol L-1, pH 4,5, isocratic
Flow rate
1,5 mL min-1
Injection volume
200 µL
Pressure
1500 psi
1000000
Unknown
peak (?)
U
120000
90000
800000
60000
600000
30000
0
400000
0
200000
5
10
Sb(V)
5
20
25
Sb(III)
0
0
15
10
15
20
25
Time, min
Figure 1. Chromatogram obtained using the PRP-X100 column for speciation of Sb in meglumine
antimoniate. Overlay 400000x diluted sample (in black) and reference solution of 50 mg L -1
Sb(III) + 400000x diluted sample (in gray) and the same chromatogram with magnification of 8x.
9
According to the chromatogram shown in Figure 1, the chromatographic peaks with PRPX100 column shows partial separation of the first peak, possibly, due to the presence of a mixture
of Sb species in nature cationic or neutral, weakly interacting with the column. This behavior can
be attributed to the presence of species of Sb. As noted (from the retention time) for a possible
presence of different species of Sb in the sample.
To try to identify the species of Sb in the sample, added to known amount of standard
solution of Sb(V) and Sb(III). You can check that none of the species present in the sample has the
same retention time of Sb(III), which is represented as the fourth peak in the chromatogram above,
however, the third peak has the same retention time of Sb(V). Thus, it can be stated that the species
Sb(V) can be identified with the LC-ICP-MS, however, is being developed another methodology
(LC-HG-ICP-MS) to try to identify the species corresponding to the first and second peaks, and the
Sb (III).
Proteomic analysis of fluids of fish as an indicator of environmental contamination
Was completed the installation of the bioanalytical laboratory in the Chemistry Department
at PUC-Rio, which consists of a system for protein separation by 2D electrophoresis, a system
HPLC-ICP-MS, Agilent and Thermo LC-MS equipment.
So far were performed the following procedures for proteomic analysis of fluids of fish as
an indicator of environmental contamination:
1. Collections of fish from contaminated sites and control to obtain the main array of study,
calculation of morpho-physiological parameters of the animals;
2. Determination of trace elements in muscle, liver and bile of fish gills by ICP-MS to
obtain correlations between these tissues and subsequent comparison of the levels in the bile of fish
with electrophoretic results;
3. Determination of metallothionein levels present in liver, bile and gills of fish;
4. Matrix clean-up for proteomic studies, including centrifugation, dialysis, desalting by
chromatography columns filled with resin Sephadex G-25 Midi-Trap with a cutoff 5 kDa and
removal of albumin and immunoglobulin by affinity chromatography columns;
5. Dosage protein matrix by the methods of Lowry modified by Peterson, Biuret, Bradford
and Kit by GE Healthcare;
6. Isoelectric focusing on Immobiline IPG strips, the separation of proteins by their
molecular weight by SDS-PAGE and testing of different types of coloration, including Coomassie
Blue G-250 and Silver;
7. Comparison of protein content, the methods of clean-up, and the results of electrophoretic
bile of two species of commercial value in the state of Rio de Janeiro,
8. Substrate zymography with gelatin and casein for determination of whether or not
metalloprotease with catalytic activity in the matrix.
CHEMOMETRY
In last decades, advances in analytical chemistry in the area of instrumentation have lead
to the development of equipments with increase capacity to obtain information about the system
under study. Using the existing convention in mathematics, each analytical instrument can be
classified according to the type of data it can provide. Zero-order instruments are those that
generate unique numeric data, a scalar per sample, are examples photometers and
potentiometers. First-order instruments are capable of generating a data vector, or an
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arrangement of first order, for each sample. These devices include all types of spectrometers,
chromatographs, and flow injection systems that utilize a single sensor. Second-order
instruments can provide an array of data per sample (tensor of order two) and data of this type
can be obtained by connecting two instruments of the first order, or by the use of multichannel
detectors. Equipments of high performance liquid chromatography (HPLC) with mass
spectrometry (HPLC-MS) and diode array detection (HPLC-DAD) are examples of these two
possibilities. Instruments of third order, by analogy, generates cubic arrays of data (tensor of
order three), but the use of such equipment is still relatively small, being an example twodimensional comprehensive gas chromatography with detection by mass spectrometry time of
flight ( GGxGG-TOFMS).
Together with the advantages in increasing the purchasing volume of information about a
system, there also arises the need for a more detailed analysis of these data. This has provided
and fostered the development of new mathematical and statistical tools for data analysis, which
constitutes the study field of Chemometrics. For quantitative determinations, where it is
necessary to establish a relationship between instrumental measures and concentration values of
species or species of interest (a procedure known as calibration), the data acquisition of first,
second and third order, led to the development of calibration methods that are able to take the
advantages of the greater amount of information.
Similarly to the classification of analytical instruments, methods of calibration can also
be grouped by the complexity of data that is generated. Univariate and multiple linear regression,
are the simplest examples of calibration methods of order zero (C0) and first order (C1). More
elaborate methods such as principal components regression (PCR) and partial least squares
(PLS) are also examples of C1, and these methods have been widely explored in recent years
because they have as main feature the possibility of analysis even in the presence of interference
that are present in the calibration samples.
The application calibration methods of the second (C2) and third order (C3) has been
growing in recent years, demonstrating their potential and ability to exploit the greatest amount
of information. Methods for calibration of second and third order have the great advantage of
allowing the determination of species of interest in the presence of interferences, even if these
interferences are not included in the calibration samples. This characteristic is called the
"advantage of the second order." Furthermore, the profile of each compound linearly
independent in the sample can be estimated from the second order data. The number of samples
required for construction of calibration models of second order is significantly less than that
required for first-order models.
Applications
Applications will be presented using molecular fluorescence and calibration methods of
second order to determine the species of interest in serum and blood plasma.
Quantification of enantiomers of ibuprofen in biological fluids
Ibuprofen (IBU) is a non-steroidal anti-inflammatory drug used as an analgesic and antithermal and to treat pain and inflammation in rheumatic problems and musculoskeletal systems.
This product, with the exception of Austria and Switzerland, is marketed as a racemate, however,
its anti-inflammatory action is associated with the enantiomer (S) - IBU. Pharmacokinetic studies
of ibuprofen have indicated that its metabolism is stereoselective with high concentrations of the
enantiomer (S)-IBU in plasma and urine. In addition, the enantiomer (R)-IBU is biotransformed
11
with inversion of configuration at the chiral center by increasing the amount of the form (S)IBU.
The techniques used to analyze enantiomers in biological fluids usually require a great
time work and a few or many stages of sample preparation. The molecular fluorescence
spectroscopy applied to this type of sample does not allow direct interpretation of the spectra due
to spectral overlap that can occur between the analyte and the components of these matrices.
However, the use of molecular fluorescence spectroscopy combined with chemometrics has an
important role in a study with such matrices, since the chemometric methods promote the
separation of signals without the need for physical separation between the inferences and
analyte.
The initial proposal was aimed at developing a global calibration model for quantification
of the enantiomers of IBU from samples of three different donors of urine and plasma. This will
facilitate the prediction of a sample from a donor who had not participated in the development
stage of the model. However, with the development of second-order model it was observed an
individual effect of matrix, suggesting that a calibration should be performed for each donor
individually. Alternatively, it was used the second order standard addition method (SOSAM). In
this method, data from emission/excitation fluorescence are decomposed using the PARAFAC
and the samples mode generates a standard addition curve. It was built a calibration model for
each sample with five successive additions in triplicate for each donor plasma and urine. For the
quantification of the enantiomers in blood plasma, the spectra were obtained in the range of 254304 nm for excitation and 312-372 nm for emission. In the case of quantification in urine, the
spectra also was obtained in the range 254-304 nm for excitation, while the emission it was
obtained in the range 338-448nm.
The results achieved by the method SOSAM showed good agreement with the expected
values. The errors found were less than 2% for the quantification of the enantiomers in plasma
and less than 5% for the quantification in urine.
Direct determination of trans-resveratrol in human plasma
The trans-resveratrol is an antioxidant found mainly in grapes and their derivatives. Many
studies have associated their intake to reduce cardiovascular disease, LDL cholesterol and
cancer. In recent years, this analyte has attracted growing interest, mainly because of its
relationship with the "French Paradox" (the fact that French people have a low incidence of heart
disease despite a diet rich in saturated fats, which is associated with the high rate of consumption
of red wine). Thus, appeared the interest in determination in plasma.
This study it was developed a new spectrofluorimetric method for the direct
determination of trans-resveratrol in human blood plasma using the advantage of second order,
which makes possible the determination of an analyte in the presence of interferences previously
unknown / not calibrated. This advantage is provided by the chemometric method of higher order
(multidimensional) called parallel factor analysis (PARAFAC). For each measure were obtained
from surface spectral bands of excitation wavelength 280-360 nm and emission 380-550 nm.
The strategy adopted in this study combined the use of PARAFAC, for extraction of pure
analytical signal with the standard addition method for determination in the presence of a strong
matrix effect caused by interaction of analyte with the proteins present in plasma, which content
varies from individual to individual. Plasma samples were diluted 10 times and for each sample
were performed four additions of a standard solution of trans-resveratrol. All determinations
were made with samples in triplicate. A specific PARAFAC model was obtained for each
sample, using a cubic arrangement of data consisting of five measures (the original sample plus 4
12
additions), 17 wavelengths of excitation and 86 emission wavelengths. The best models were
selected with four factors and explained more than 99.90% of the total variance.
The weights (loadings) obtained were attributed to trans-resveratrol and three different
interferences, including tryptophan. The scores for the analyte were used in linear regressions
and all the standard addition curves showed correlation coefficients of at least 0.99. Good results
were obtained for the concentration range from 0.10 to 5.00 µ g mL-1, with the degree of
recovery ranging from 95.5% to 110.0%. The method was validated by estimating figures of
merit such as sensitivity, analytical sensitivity, selectivity, precision, limits of detection and
quantification. To estimate these figures were used expressions recently proposed, since the
estimation of figures of merit for higher-order methods is still controversial in the literature and
research.
SEPARATIONS
“Detection and Identification of Secondary Metabolites from Plants by µHPLC and
GC×GC”
a) Importation and instalation of GC-MS QP2010+: The main goal of this sub-project is to set up
GC×GC-qMS systems for the characterization of secondary metabolites from plant species of
interest. This system was imported with the financial support from this Grant and was installed
in December 2009 at LCG/IQ-Unicamp. It is currently under evaluation, operating in the
conventional mode until the GC×GC module is ready and available.
b) Project and assembly of conversion kits of GC-MS to GC×GC-MS: Based on our previous
experience, we projected the modules required for the conversion of the GC-MS to a GC×GCqMS. We redesign the electronics and the hardware for the temperature control and the
command for the gas valves. A new cryogenic modulator was assembled and improved over the
previous version. Such devices were initially installed in a GC-FID at LCG/IQ-Unicamp to
evaluate initial performance and eventual corrective adjustments (Figure 1). Once the modules
are working properly, they will be transferred to the GC-MS QP2010+, and doubled for the
assembly of a second system at LPCEA/DQ-UFSC.
Figure 1. Modules
for
control
and
supply of cryogenic
fluid (esquerda) and
cryogenic modulador
installed in the oven
for a GC-FID.
c) Preliminary assay: study of the volatile fraction of Anona cosmosus by HS-SPME-GC-FID:
Parallel to the assembly of the GC×GC-qMS system,
we performed preliminary studies aiming to establish a correlation of retention indexes obtained
from single dimension GC system and in GC×GC system, using for that the GC×GC-FID
13
available, and confirming with conventional GC-MS. As a substrate, we used pulp from
pineapple (Ananas cosmosus L.), fresh and dehydrated.
Figure 2 show a section of one chromatogram obtained in GC×GC-FID for dehydrated pineapple
pulp. The yellow line indicates the position of the peaks for the homologous series of methyl
esters of aliphatic carboxylic acids (pentanoate to nonanoate). Some peaks, e.g. methyl
heptanoate (C8) and methyl nonanoate (C10), were not identified by GC-MS due to coelution
with 3-methyltiopropanoate and the poor quality of the mass spectrum, respectively. The
identification of such compounds was only possible due to confirmation of existence of signal in
the expected range in the chromatogram GC×GC-FID.
Figure 2. Chromatogram GC×GCFID from the volatile fraction of the
dried fresh pulp from pineapple.
Another relationship was found
between aliphatic methyl esters and
the isomeric ethyl esters (lines
yellow and green). It was possible to
observe that chromatograms from
GC×GC-FID presented a constant
relationship among the retention of
isômeros in homologous series.
“Chemometric Evaluation of Urinary Peptidic Maps by CE-MS Aiming Diagnostics of
Vesicoureteral Reflux (VUR)”
This research aims for the establishment of a non-invasive diagnostics of VUR by the analysis of
urinary peptidic maps generated by the coupling of capillary electrophoresis and mass
spectrometry (CE-MS). Analysis of urinary peptides aiming clinical diagnostics is well
documented in the literature. More than 600 polypeptides can be found in a single CE-MS run
from urine samples [1]; in another work, researchers found 1400 polypeptides in urine and could
distinguish three groups (normal, benign pathology, a malign pathology) among prostate cancer
patients, what is promising results in the area of non-invasive diagnostics [2]. In 2005 a paper
was published [3] using CE-MS in the diagnostics of ureteropelvic junction, detecting momre
than 2000 peptides. In 2007, another paper using the same technique established biomarkers for
disease in the coronary artery [4]. Figure 3 shows tri-dimensional maps of samples from urine of
a healthy child and one from a VUR positive child, under the optimized CE-MS conditions.
14
a)
b)
Figure 3. a) Peptidic Map obtained urine of a three-year-old girl with VUR and b) a peptide map
form a healthy boy that is four-years old, under the optimized conditions.
Due to the limited computational resources for the study of clusters, the original data, with more
than a million coordinates (time, intensity, and mass) was simplified. Archives of several sizes
(5-750 mare intense signals) were generated using three variables (time, intensity, and mass),
and two variables (time and mass). None of those files provided the capacity to distinguish the
health group from samples form those that are VUR positive. A graph with 150 data points,
containing the top 30 most abundant peaks coordinated by time, in each spectrum. The top 5
most intense peaks were selected as and example (Figure 4).
In Figure 4 is possible visualizing that
healthy children presented peaks at low m/z
values at the beginning of the run.
Oppositely, the positive control samples
demonstrated peaks of greater values of m/z
throughout the rum. It is clear that the
logical choice is the selection of the peaks
according to their intensity in function of
time and to apply a pattern recognition
method using the mass/charge ratio.
Figure 4. Tridimensional graph (samples vs
intensity vs m/z). Samples from 1 to 23, and
36 are positive to VUR. All others are
negative.
PCA, or Principal Component Analysis, is a method based in standardized linear combination
(SLC – standardized linear combination) that searches by means of orthogonality, to explain the
differences among the original data. For this method, we used the same file containing three time
slots, and in each slot selected the 10 most intense peaks, totalizing 30 peaks. Figure 5 revels the
results obtained, utilizing the five most relevant peaks. The error rate was the same obtained with
PAM, that is, 23% of samples were classified as wrong. Other parameters were similar, such as
sensitivity of 75% and specificity of 80%.
15
-2
0
2
4
6
Figure 5. PCA from the 30-most intense
peaks, using the first 5 peaks.
4
2
0
11
10
20
34
3
65
14
1
15
31
4 31
16
13 18
Col4
Col3
-2
Comp.2
0.2
38
39
35 12
30
24
23
22
21
19
33
29
36
25
32927
28
38
39 37
26
0.0
9
19
21
22
23
36
17
-0.4
Col2
-4
35
8 Col6
Col5
-0.2
24
25
26
27
28
29
30
32
33
0.4
7
6
0.6
-4
2
-0.4
-0.2
0.0
0.2
0.4
In conclusion, the coupling of CE-ESI-MS is
an advantajous procedure to obtain peptidic
maps from urine samples. In this preliminary
study, it is clear the correlation among the
urinary peptidic maps in the universe of
samples used. In the future, to confirm this
technique as a non-invasive diagnostic method,
a broader spectrum of samples will be
required.
0.6
Comp.1
References
[1] Kaiser, T., Hermann, A., Kielstein, J. T., et. al., J. Chromatogr. A, 2003, 1013, 157-171.
[2] Theodurescu, D., Fliser, D., Wittke, S., et. al., Electrophoresis, 2005, 26, 2797-2808.
[3] Haubitz, M., Wittke, S., Weissinger, E. M., et. al., Kidney Int., 2005, 67, 2313-2320.
[4] Zimmerli, L. U., Schiffer, E., Zürbig, P., et. al., Mol. Cell. Proteomics, 2008, 7, 290-298.
“Metabolome: Investigation of Tumor Markers Profile by Capillary Electrophoresis and
Mass Spectrometry”
In the time frame for this report, some preliminary studies were carried out. Standards of
modified nucleosides (tumor markers investigated in this sub-project) were used to optimize the
analytical conditions for HPLC e CE, both with UV-vis detection. The analytical standards were
thymidine, cytosine, adenosine, guanidine, 2’-deoxyadenosine, inosine, N4-acethylcitidine, 5metiluridina e 1-methyladenosine, 8-bromoguanosine (internal standard). For the separation
using CE, we evaluated the parameters: i) pH and concentration of the background electrolyte
(BGE); ii) wavelength for detection; iii) separation voltage; iv) concentration of surfactant; v)
concentration of organic modifier. Figure 6 present the electropherogram after optimization of
the parameters. The electropherogram presents great efficiency and analysis time. The
resolution, however, is still limited for the adequate separation of all analytes, in particular N4acethylcitidine and 1-methyl-adenosine. The next steps will focus on the extraction conditions,
validation, and real samples. For HPLC analysis, the optimized parameters were: i) stationary
phase, ii) strength of the mobile phase (MF), iii) nature of MF, iv) composition and pH of
aqueous MF, v) flow rate and vi) gradient of MF. Figure 7 presents a chromatogram obtained
from the best analytical conditions.
70
2'-Deoxiadenosina
60
Adenosina
Citidina
Absorbância (mAU)
50
1-Metiladenosina
40
N4-Acetilcitidina
5-Metiluridina
Timidina
30
Guanosina
20
Inosina
8-Bromoguanosina
Figure 6: Electropherograms of nucleosides
modified and not modified in BGE borate 20
mmol L-1 pH 9.2, SDS 300 mmol L-1 and
15% CH3OH. V: 25 kV; tinj: 15 s (50 mbar);
λ: 260 nm; fused silica capillary: L: 60/52
cm; internal diameter (i.d.): 50 µm. Acetone
was used as EOF marker.
10
0
0
5
10
Tempo (min)
15
16
50
140
8-BromoG uanosina
40
100
1-metilAdenosina
Inos ina
80
C itidina
A denosina
30
2-deoxiAdenosina
%B
Absorbância (mAU)
120
5-metilU ridina
60
20
40
Timidina
N4-acetilC itos ina
Figure 7: Chromatogram obtained under
optimized conditions. Column ACE C18 25 cm
x 4,6 cm x 5 µm; mobile phase A phosphate
buffer 10 mmol L-1 pH 5; mobile phase B
methanol/water 70:30 (v:v), flow rate 1 mL min1
; λ: 260 nm; gradient represented by the red
line.
20
10
0
After optimization, an analytical curve
was
made
with parameters such linearity (r =
tempo (min)
0.9947 for adenosine to 0.9972 to N4-acethylcitidine) and limits of detection/quantitation (LOQ
≈ 10-7 mol L-1 for all analytes). The method for extraction of the nucleosides is under evaluation
and next step will be the validation of the method.
-20
0
5
10
15
20
25
30
35
40
45
50
55
0
“Preparation of Monolithic Columns for HPLC and CE”
In this period we prepared and evaluated electrophoreticaly the capillaries with monolithic
stationary phase from octadecylmetacrylate in different types and porogenic solvent ratios. The
columns were evaluated electrophoreticaly using a mixture of alkyl benzenes. The column that
presented the greatest chromatographic efficiency for the compound most retained was prepared
with porogenic amyl alcohol: 1,4-butanediol 65:35% v/v. We observed that an raise in 1,4buthanediol in the mixture yielded columns with best chromatographic efficiency. The results
indicated the viability to obtain monolithic columns prepared with octadecylmetacrylates with
good efficiencies and great separation of compound that are not polar. We also synthesized and
characterized a monolithic column for electro chromatography using the sol-gel method. Due to
the great number of possibilities in the stationary phases, in this work we proposed a new
sililating agent with carboxylic groups in the structure, formed by acid para-aminobenzoic and
triethoxypropyl isocianatesilane, for many reasons: 1) proposal of a new phase, 2) the phase
must provide electroosmotic flow, 3) the phase must carry some aspects of cation exchange, (4)
propose a new phase with a great variety of applications. The synthesis was carried out using
acid 4-aminobenzoic (PABA), in equimolar quantity with triethoxypropylisocianatesilano and
triethylamina em N,N-dimethylformamide (DMF). After this step, the monolithic columns were
funcionalized with sililant agent, characterizade with spectroscopy, microscopy and
eletrophoretic. The column provides the separation of a few aminoacids and basic drugs. During
this period, we initiated the project related with extraction of antioxidant in carqueja. The
optimization is undergoing, as well as the extraction techniques such as liquid-liquid extraction,
and lab-made solid phases.
“Instrumentation and Miniaturization of Electrophoresis Systems”
We have been working on the development of instrumentation and methods for CE and
analytical microsystems. Since beginning of the INCT several studies were initiated and are in
the development stage, with preliminary results. We have started the studies of coating of glassy
surfaces with layer-by-layer assembly using chitosan and sodium alginate. The main goal is to
prepare the surface of fused silica capillary tubes to eliminate or control de EOF and to minimize
17
protein adsorption of analytes. We observed a reduction of the hydrophilic caracter of glass
slides by measuring contact angles. Up to five alternating layers were coated, but the best results
were found with three layers. The EOF was completely suppressed, what is a promising result
and we are now investigatin the stability of the layers. We are assembling an equipment for
interfacing flow systems and CE. This opens new perspectives for the automation of sample
preparation (derivatization, clean up, degradation, concentration, etc.) on line with a separation
system. Furthermore, this same instrumentation will allow the coupling of another separation
method as a different dimension of separation such as in bidimensional systems (for example,
size exclusion chromatography and capillary electrophoresis). We intend to use this instrument
for enzymatic studies of compounds and the analysis of the products. We are also developing a
method for determination of lapachol using CE and UV-vis detection. The method presented
good selectivity for lapachol, which was evaluated by its spectral purity, obtained from
supercritical fluid extracts of ipe bark (Tabebuia gender). This method is under validation and
the optimization of the extraction method and will be presented at the ITP2010 Conference in
August, in Baltimore, Maryland, USA (http://fp.okstate.edu/2010/). Another method is under
evaluation for determination of sodium alginate by CE with contactless conductivity detection.
We’ve obtained a good separation condition and we are now carrying out the validation of the
method, and this work will also be presented at ITP2010.
“Tools and Methods for Chromatography and Electrophoresis for the Detection and
Quantification of Biomolecules”
Genomics: Genetic profiling of Alicyclobacillus acidoterrestris: The molecular classification of
nineteen strains isolated from different acid juices and fruits were studied using RAPD-PCR.
This technique produces polymorphic fragments, which were analyzed by CE in microchips and
bioinformatics tools. With the aid of a program, Phylip v.3.66, we constructed phylogenetic trees
to group and differentiate the isolated strains. Simultaneously to the phylogenetic analysis, we
carried out the DNA sequence analysis of variable region of the 16S rRNA gene of A.
acidoterrestris. The strains investigated showed 99% in similarity overall. We further applied
molecular methods to investigate the inhibition of vegetative cells and spores of A.
acidoterrestris. We combined thermal treatments and saponins—a secondary metabolite
extracted from Sapindus saponaria tree with biotechnology potential—and observed inhibition
of the bacteria up to 99.6%. We want to further investigate the effects of adding this extract as
antibacterial and food preservative, and understand how is the mechanism of action of the
saponins, in systems biology approach.
Proteomics: The project “Bi-dimensional Electrophoresis as a Fundamental Tool in Proteomics”
financed by FAPESP (Process # 2008/04050-4) gives the INCT Bioanalytical Chemist a
matching counterpart in basic instrumentation for separations of proteins by bi-dimensional
electrophoresis. We are carrying out the characterization of proteins related to neurological
disorders. The project “Determination of phospholipases A2 blood samples from patients with
neuropsychiatric disorder” aims to identify and quantify the iPLA2 in platelet samples of patients
and controls in order to respond if exist differences in abundance or activity of iPLA2 from both
groups. The separation of the proteins is carried out by 2D-Electrophoresis and detected by
Western blotting and mass spectrometry. The separation parameters are optimized and we will
start to compare samples from patients and controls. If our hypothesis is correct this project has
the potential of producing the first non-invasive diagnostics for schizophrenia. Another project
involves glycomics of α-dystroglycan (α-DG) and the glycoprotein profiling using dystrophic
18
animal models. We carry out the differential proteomics approach to study α-DG from muscle
tissues from healthy controls and genetic variants. We are developing the bioanalytical methods
for selective concentration and purification of proteic extracts with different lectins bound to
agarose columns, such as concanavalin A and jacalin. Promising results were obtained with
wheat germ agglutinin (WGA) columns, with many differentiated spots were found between
health and disease tissues.
Metabolomics: We study the metabolism of grapes to monitor the metabolic profiling with the
ripening and the close correlation with the protein and genetic profiling with such process. The
goal of this project is to follow the maturation of the grapes produced in Água Vermelha, São
Carlos, SP—which is a region with good potential vitiviniculture activity given the climatic
conditions and the experimental handling of soil and trimming—by means of the metabolicproteomic correlation during the ripening process. The main organic acids, responsible for the
acidity of the must, found in grapes using HPLC were tartaric, malic, and citric. The
concentration of such acids decreased during maturation process, as well as the total acidity of
the grapes. The respiration process mainly regulates this behavior with the malic acid, and the
dilution effect induced by the enlargement of the process of the grape. In the proteomics
counterpart, we investigated different protein extraction methods for 2D-Electrophoresis because
this sample is rich in polyphenols, which are known to interfere in the protein extraction process.
Best results were obtained with trichloroacetic acid (TCA), or with phenol/ammonium acetate.
Metabolomics is the field that the new CE equipment will be most valuable, as illustrated in
Figures 8 and 9. In these two figures, we show examples of separation de secondary metabolites,
and CE can offer new insights related to systems biology and bioanalytical chemistry.
Figure 8. Exploratory analysis of
mandelonitrile, a racemic substrate used in
the investigation of the enantioselectivity of
the enzyme hydroxinitrileliase, an enzyme
produced by Xylella fastidiosa, apparently
involved in defense mechanism against
cyanogensesis.
Figure 9. Electropherogram of the mixture of
sugars used to monitor the maturation
metabolism of grapes. (1) sucrose, (2)
galactose (3) glucose (4) rhamnose (5)
fructose (6) xylose.
19
d) Miniaturized Systems for Analysis of Biomolecules: We introduced a new solid-phase DNA
extraction tecnique in microchips. This technique isolates pure DNA, which is amplification
ready, directly from complex matrices, such as blood. The dynamic extraction method in solid
phase extracts DNA in a microchip made of polyester-toner (PeT) assembly turned out a
efficient extraction method for DNA because combine advantages as speed (extraction in about
10 min) and yield of DNA (extraction efficiency up to 70%). In face to such features we
postulate that the disposable PeT microdevices can became a new generation of microchips
dedicated to genetic analysis because is an alternative that is i) simple, ii) low-cost, and iii)
integrated—we are developing a device that bares extraction, amplification, and separation of
DNA on a single, disposable device.
We are developing other microanalytical systems that incorporate microfluidics and
biosensors. We introduced for the first time the use of capacitively coupled contactless
condutometric detection (C4D) as a biosensor. We are now introducing such biosensor for the
detection of cancer biomarker FR-α in hybrid microchip made of glass/SiO2/PDMS
incorporating the C4D biosensor for flow analysis (µBIA-C4D), using folic acid (FA) as the
bioreceptor. The construction of the µBIA-C4D used planar and rectangular electrodes that
facilitate the bonding of the microchip, and avoid the need for cleaning of the electrodes
(contactless electrochemistry). Our results indicated a successful functionalization of the FA
over the electrode surface using self-assembly processes. In the following experiments we are
going to probe real samples to evaluate the interactions between FA/FR-α. This method carries a
promising tool for diagnostic of cancer and to monitor the success of treatments. We are
developing new techniques for production of analytical microdevices made of glass. An
alternative process seals the glass layers onto the microsystem and simultaneously isolates the
electrodes for C4D. This process etchs channels in glass using photolithograpy and wet chemistry
etching. Then a thin layer of PDMS isolates the electrodes, on the bottom glass chip, from the
etched channels, on the top glass chip. The analytical performance of such devices were
measured using C4D and also using laser induced detection (LIF). The separation efficiency was
about 47000 plates/m with good repeatabilidade from chip-to-chip. This new bonding process
was used in a enzymatic reaction to study the kinetics (Vmax = 12,64 mM min-1 and KM = 23,8
mM) for the reaction of decomposition of urea catalised by urease. Ammonium ions was
monitored using C4D system.
(Bio)sensors
Brief description of the main results obtained during the period is listed and descriptions
of the main results obtained by the various projects of the group of sensors. Several cases, these
results are already included in the publications and presentations in various events.
The project led by Prof. has been working with fluoresecentes sensors to detect free
radicals that are widely used in studies on free radicals and reactive species. Among the most
used is dihidrorodamina 1,2,3 (DHR). This work proposes to DHR in immobilization of gold
electrodes with the aim of obtaining an electrochemical sensor for free radicals. The electrodes
used were fabricated using gold compact discs (CDs. Then a layer of thiols were immobilized
onto the electrodes containing the carboxyl group and after that, the DHR was immobilized on
the modified electrode. The electrodes made from gold CDs were very easy to clean. The typical
profile of gold was obtained with only a few cycles of electrochemical cleaning. They were
20
tested for the formation of self-assembled monolayers with mercapto-octanoic acid, mercaptomercapto-decanoic and hexanoic by immersing the electrodes during different times in these
solutions. After modification of gold electrode with mercapto-decanoic acid, the electrode was
immersed in a solution of 20 mmol L -1 DHR for 1 hour. Then, we performed cyclic voltammetry
in a solution containing only supporting electrolyte (KCl 0.1 mol L -1) and the resulting
voltammogram is shown in Figure 1. There is a redox process - a reduction peak around 0.25 V
and an oxidation peak around 0.35 V. As in the solution used in the electrochemical cell was
only the supporting electrolyte (KCl), which has no redox activity, the pair of peaks is probably
related to the DHR. Therefore, this result indicates that the DHR was immobilized successfully.
60
40
i / µA
20
0
-20
-40
0,0
0,1
0,2
0,3
0,4
0,5
E / V vs Ag/AgCl
Figure 1: Cyclic voltammetry in 0.5 mol L -1 KCl electrode modified with mercapto-decanoic
acid after immobilization of the DHR.
Results of cyclic voltammetry, electrochemical quartz crystal microbalance and infrared
spectroscopy confirmed the detention of DHR in the electrodes. This work is ongoing.
Another project that is being developed in UFABC is led by Professor Wendel Andrade
Alves and developmental stage of the current research indicates that we establish conditions for
the production of peptide nanostructures like nanotubes and nanofibers, and nanotubes of
titanium oxide, aiming its application in the development of new electronic devices. Moreover,
we have conducted studies of functionalization of the walls inside the nanotubes with gold
nanoparticles and / or conducting polymers and how this influence on the electronic structure
and transport properties of these systems. The results indicate that we have managed to
functionalize these materials, but larger studies are still needed, for example, varying the
concentration of nanoparticles and functionalization of these materials on the surface of the
electrodes. These results are part of projects for master students of the post-graduate program in
Nanoscience and Advanced Materials under the guidance of Prof. Alves at UFABC. We believe
that our results are of great importance and relevance in this area because we develop a new
method to control the functionalization of peptides that is not described. The partial results
obtained by the group are part of a research line under development in the laboratory with
support from FAPESP (Research Assistance - Young Researcher, Proc. No. 2008/53576-9 ), the
21
project titled "Synthesis, Characterization and Study of Electronic Properties of Peptide and
titanium oxide Nanotubes. In addition, we also studied the immobilization of copper (II)
complexes containing tridentate ligands of Schiff base type in Nafion ® membrane on the surface
of a glassy carbon electrode for the construction of biomimetic sensors of multinuclear copper
(II) enzymes. The results obtained by the group indicate that tetranuclear copper (II) species are
better electrocatalysts than mononuclear compounds. Cyclic compounds seem to facilitate
interaction of the substrate with the metal center. This work also suggests that the copper (II)
complex provides a cyclic process of electrocatalytic reduction of O 2 by a mechanism involving
4 electrons. These results were recently accepted for publication in the journal "Electrochimica
Acta", and are related to the work of masters, recently defended by a student who already has a
PhD scholarship from FAPESP to continue these researches.
In the projects led by Profs. Flavio Damos (UFVJM, Diamantina) and Auro Tanaka
(UFMA, Maranhão) work in the area of sensors prepared by layer-by-technique combined with
SAMs layer has allowed to develop sensors of dissolved oxygen through the combination of
nanoparticles and supramolecular complexes. These works have been published in indexed
journals and are listed below.
The project developed in São Paulo (USP) is led by the group of Professor Susana
Cordoba de Torresi and has been working on developing different nanostructured platforms for
applications in molecular recognitionFor this, we have worked to obtain mesoporous films of
different materials that can be functionalized with different electroactive molecules or
biomolecules. A new line is being implemented with the use of ionic liquids for the
immobilization of biomolecules. Thus, with the modification of ionic liquids, specifically its
hydrophobicity is intended to optimize the microenvironments of enzymes or proteins in general
in order to obtain sensors more sensitives, specifics and stables.
The project developed in Ribeirão Preto (USP) by the group of Professor Rose Naal, has
been working on the development of cellular biosensors to study the antiallergic activity of
natural and synthetic substances. Due to the severity of allergic diseases, lack of appropriate
treatments and the high percentage of people who suffer from various forms of this condition,
there is intense interest in the pursuit and development of substances able to inhibit the allergic
processes. Within this context, various substances (natural or synthetic) have been investigated
through the model biosensor based on mast cells. Among them we can highlight a homologous
series of aryl coumarin derivatives (Figure 2) which appear as promising compounds for the
treatment of disorders of this nature, as well as its easy synthesis, these substances share much
structural similarity with flavonoids, whose anti-allergic activity have been extensively reported.
So we tried to, during that period, to establish a relationship between the major structural
requirements needed for the inhibition of mast cell degranulation. RBL-2H3 cells sensitized with
anti-DNP-IgE were stimulated with the antigen DNP-BSA in the presence and absence of
substances of interest. The release of the enzyme β-hexosaminidase was quantified using the
fluorogenic substrate umbeliferil-methyl-N-acetyl-β-D-glucosamine. The results showed that
from the collection of 33 synthetic coumarins investigated, 18 had inhibitory effect on the release
of β -hex, in a dose-dependent, whose power was higher or similar to the positive control
(ketotifen fumarate, IC 50 = 15 mM). The presence of amino and nitro groups on carbon 6 of 3Piperonyl-coumarins (Figure 2-A) implies active compounds whose potency is increased when
the side chain of this substitution is extended by the introduction of heterocyclic aromatic rings
such as thiophene, pyridine and indole. While hydroxylations have no positive response on the 3Piperonyl-coumarins, the hydroxylations of positions 6, 3 'and 2' of the phenyl-coumarins
(Figure 2b) seem to be important for the activity. Regarding esterifications, no, in general, gain
22
of activity in the presence of this function was observed. Was evaluated, even if the inhibitory
response observed was due to the inhibition of mast cell degranulation and consequent negative
modulation of enzyme release, or if there was actually inhibiting the activity of the enzyme βhex. The results showed that the enzyme is directly inhibited only at higher concentrations
(above 50 mM) of coumarin compoundsThe results suggest that arilcumarinas can be identified
as potential compounds for the treatment of allergic disorders and / or also provide a promising
prototype for the development of new molecules with therapeutic potential.
5´
5´
O
R1
3
1´
6
R3
7
O
8
2
3´
O
O
R4
R1
R3
1
R3
(A)
3
6
7
O
8
2
1´
O
3´
R5
R6
1
R3
AC-1: R 1=R2=R 3=H
AC-17: R 1=R2=R3=R4=R5=R6=H
AC-2: R 1=OH, R2=R3=H
AC-18: R 1=OH, R2=R3=R4=R5=R 6=H
AC-3: R 1=R3=H, R2=OH
AC-19: R 1=R2=R3=R5=R6=H, R 4=OH
AC-4: R 1=R2=H, R3=OH
AC-20: R 1=R2=R3=R4=R6=H, R 5=OH
AC-5: R 1=R2=OH, R3=H
AC-21: R 1=R4=OH, R2=R3=R5=R 6=H
AC-6: R 1=H, R 2=R3=OH
AC-22: R 1=R6=OH, R2=R3=R4=R 5=H
AC-7: R 1=OCOCH3, R2=R3=H
AC-23: R 1=R2=R5=R6=H, R3=R 4=OH
(B)
AC-8: R 1=R2=H, R3=OCOCH 3
AC-24: R 1=R4=R5=H, R2=R3=R 6=OH
AC-9: R 1=R2=OCOCH3, R3=H
AC-25: R 1=R2=R4=R5=OH, R3=R 6=H
AC-10: R 1=H, R 2=R3=OCOCH3
AC-26: R 1=OH, R4=OCH3, R 2=R3=R 5=R6=H
AC-11: R 1=NH 2, R2=R3=H
AC-27: R 1=OH, R6=OCH3, R 2=R3=R 4=R5=H
AC-12: R 1=NO2, R 2=R3=H
AC-28: R 1=OCOCH3, R2=R 3=R4=R5=R6=H
AC-13: R 1=
NH
R2=R3=H
AC-29: R 1=R4=OCOCH3, R 2=R3=R 5=R6=H
NH
R2=R3=H
AC-30: R 1=R5=OCOCH3, R 2=R3=R 4=R6=H
R2=R3=H
AC-31: R 1=R6=OCOCH3, R 2=R3=R 4=R5=H
O
AC-14: R 1=
S
AC-15: R 1=
NH
N
AC-32: R 1= OCOCH3, R4=OCH3, R 2=R3=R5=R 6=H
H
N
AC-16: R 1=
NH
R 2=R3=H
AC-33: R 1=OCOCH3, R6=OCH3, R 2=R3=R4=R5=H
Figura 2. Estruturas químicas de 3-piperonil (A) e 3-fenil-cumarinas (B), com suas diferentes substituições.
Besides the synthetic coumarins, other natural substances of different classes, were studied.
Among them we can mention: 1) Flavonoids: 7-Methyl-quercetin and mangiferin Teprowatsina
A, 2) Alkaloide:  3) polyphenolic acids: caffeic acid and 4) Iridoids: Secoxilaganina. The IC 50
values obtained for these substances are in the range 4.8 to 15.9 mM, indicating generally good
inhibitory potential, especially the 7-methyl-quercetin (4.8 ± 1.0 mM) . However, this flavonoid
showed high toxicity to the cell (45% of death by 10 mm), and inhibit the activity of the enzyme
β-hex, which decreases its potential use as an antiallergic. The other substances did not show
high capacity inhibition of the enzyme (2 to 10%), but the caffeic acid and were more cytotoxic
than the others with 40 and 31% death, respectively, 10 mm of the substance. All substances
tested showed high inhibitory potential, but only mangiferin, and secoxilaganina teprowatsina-A
23
showed low cellular toxicity and little inhibition of β-hex, ensuring greater potential for use as an
antiallergic.
Inclusion complexes of flavonoid-β-CDs for the use in allergy testing
S0,
Log P εc, MComplexo
Kc
1
cm-1
µM
inclusão
(M-1)
Hesp/βCD
175
24300
35
0,83
Hesp/2-HP38230
βCD
330
Mor/βCD
1412
39100
50
1.18
Mor/2-HP40180
βCD
3121
Nar/βCD
1940
21728
70
1.99
Nar/2-HP-βCD 3199
23871
Table 1. Constants (kc) of flavonoids in β-CD and 2hydroxypropyl-β-CD, values of water solubility (S 0) at
25 ° C, and hydrophobicity (log P) obtained by the
mathematical model ChemDraw .
Many natural substances of biological interest are poorly soluble in water, which reduces the
absorption in the body. Moreover, they may be susceptible to photodegradation or photooxidation, which reduces the efficiency of the assay and the therapeutic effect. The formation of
inclusion complexes with cyclodextrins (CD) can minimize such problems and contribute to the
success of studies focused on finding new drugs. Thus, there were certain constants incoporação
flavonoid hesperidin, naringenin and morin in two cyclodextrins, the β-CD and 2-hydroxypropyl-β-CD, while the latter is more suitable for biological applications because of its lower
nephrotoxicity compared the β-CD (cheaper and used as a model. The results gathered in Table
1 show that the incorporation of the natural substance in the CD is favored by the higher
hydrophobicity of the same (lower solubility (S 0) and lower log P). The change in the structure
of β-CD favors the incorporation of the drug and leads to the formation of more stable
complexes (higher Kc) with a consequent increase in solubility. Finally, we determined the
coefficients of molar absorptivity of inclusion complexes (ε c) for the determination of their
concentration in biological assays future.
Electrochemical studies of benzodiazepine derivatives
Work of the group of prof. Zeki Naal Benzodiazepine derivatives, such as flunitrazepam
(FNTZ), has pharmacological activity on central nervous sistemma and is widely administered as
an anxiolytic, anticonvulsant, muscle relaxant and anesthetic-sedative. Most of the biological
characteristics of these substances depends on metabolic reactions that lead to the reduction of
the nitro group. Another example, whose activity is ant-inflammatory nimesulide (Nimes), which
also has a nitro group redox active. It is also known that reducing the nitro group can be
catalyzed by derivatives viologênio i.e, reduction of nitro derivatives and alpha nitrocetonas by
24
sodium dithionite mediated dioctilviologênio. Therefore it is of interest to study the redox
behavior of derivatives to reduce viologênio mediated nitro groups of drugs as FNTZ and Nimes.
this period were obtained electrodes modified by electropolymerization of viologênio
derivatives of N, N'-di (3-pyrrol-1-yl-propyl) -4.4 '-bipiridinium (dppb) and N-(3-pyrrole-1 -ylpropyl) -4.4 '-bipiridinium (PPB) and eletroadsorção and precipitation of bromide
polibenzilviologênio.
A
B
Figure 3A shows the cyclic voltammograms of a phosphate buffer pH 7 with 0.1 mmol L -1 of
nimesulide obtained with the plain gold electrode (blue) and the modified electrode
polibenzilviologênio. The great decrease in reduction potential of nitro group suggests a high
catalytic activity of the film. However these experiments require confirmation after synthesis of
the polymer. The same was observed for the electrode electropolymerized with dppb (Fig. 3B) in
the reduction of flunitrazepam. In this case the variation of potential for the process of reducing
the nitro group was lower than the previous case (from -0.6 V to -0.45 V), but enough to justify
the development of a device for analysis of drug question. In this case also needed synthesis of
the monomer for the continuation and completion of this study.
The redox signaling is evolving as a new field of biochemistry and pharmacological
research. The most important component is the gearbox as a necessary part of a reversible
regulatory process. Over the past three decades, it became increasingly clear that intracellular
signaling pathways are activated by changes in the oxidation / reduction (redox) metabolism
involving intracellular reactive oxygen species (ROS), ie, superoxide / hydrogen peroxide, etc.
One way to monitor the reaction between oxygen and other molecules is the electrochemical
generation of superoxide / peroxide by reducing oxygen at the electrode surface. However
oxygen is poorly soluble in aqueous solution at physiological pH, so the current signal is very
low, and the potential is around -0.6 V vs. Ag / AgCl (s) / KCl (sat) that transforms electrochemical
processes rather selective. The chemically modified electrode is one of the most important
research topics in electroanalytical area. It is known that viologênio is a mediator for the
reduction of oxygen to superoxide. We also studied the electrode modified with PBV as the
reduction of molecular oxygen. Cyclic voltammograms of glassy carbon electrode (4A) and
carbon fiber (4B) modified with PBV in the presence of O 2 in phosphate buffer pH 7 showed a
catalytic behavior as the reduction of oxygen. A significant increase in peak current of the
25
cathodic and anodic peak of their disappearance, suggesting a catalytic process in the reduction
of oxygen (O2) and superoxide (O 2 -), in an aqueous solution reaction has the equation:
O2 + HO2- + OH-
2O2- + H2O
K = 9,1 x 109
This result indicates that this chemically modified electrode can be used in biological studies
involving reactive oxygen species.
A
B
Sensors based on ceramic materials and modified silicas.
Yoshitaka Gushikem group aims at the development of electrochemical sensors based on
ceramic work has been done where we attempted to prepare composite substrates (composites) to
present low electrical resistance. Through sol-gel processing, C-graphite particles of nanometer
dimensions have been incorporated in the silica matrix resulting in the material SiO 2 / ZrO 2 / C
and SiO 2 / TiO 2 / Sb 2 O 5 / C. microscopy images Transmission electron show that they are
homogeneously dispersed in the matrix ensuring good connectivity In a similar study, the
ceramic material SiO 2 / SnO 2 / CoPc was prepared, where is the phthalocyanine CoPc Cobalt
(II) which was prepared in situ in the pores of the matrix SiO 2 / SnO 2 to prevent the species of
developing clusters within the matrix. In this procedure, the CoPc is confined in the pores of the
ceramic material, thus avoiding in processes of electron transfer reactions occur on the surface
interactions between the electroactive species. A work of great interest was completed and
published where the ion NO 2 - was oxidized electrochemically directly on a thin polymer film
silsesquioxane grafted on SiO 2 with chloride ions of n-propilpyridinium  Si (CH 2) 3-Py + Cl - .)
Oxidation occurs with the ion NO 2 - confined in the network of polymer matrix and therefore
free from interference of other ions, independent of pH in the range between 3 and 10. In this
case, an electrode modified with carbon paste, was very efficient for oxidation and analysis of
this anion. In the part of the project consisting in new adsorbent materials, the silsesquioxane
polymer modified with pyridinium ion was used in adsorption of this metal halide in ethanol.
Because this is a work of great interest for better understanding the mechanism of adsorption at
the interface of solid-solution, the equilibrium constants and the chemical species adsorbed were
studied. Finally, an IC student from another institution developed over a period of three months,
26
a work on LbL films having as a functional group, R-SH on a gold disc and applied in the
oxidation of hydrazine.
Nanostructured materials for platforms of sensors and biosensors
Imprinting molecular recognition systems (sensors); A system of molecular imprinting
has been obtained to recognize phenolic compounds with the strategy of combining a catalyst
along with the recognition site, giving rise to a material similar to the peroxidase activity In a
next step was made immobilization of molecularly imprinted polymer on a glassy carbon
electrode using Nafion as an adhesive agent polymer with the surface of glassy carbon. The
selectivity obtained with these materials for 4-aminophenol was much higher than those
observed with peroxidases. Insertion of nanoparticles of inorganic materials in thin films of
polymer, silica and ceramics, to obtain materials with conductivity suitable for the construction
of electrochemical devices. The search for insertion of nanoparticles of inorganic materials in the
modification of electrodes led to some interesting studies. In the first line of action was obtained
gold nanoparticles on gate of the field effect transistors for the search of increased sensitivity of
the ISFET (Ion Selective Field Effect Transistors) which allowed the publication of two articles
in journals of international circulation. On another front showed the effect of metal nanoparticles
on carbon nanotubes immobilized on the electrode surface to obtain electrochemical sensors and
biosensors. On this front the results gave rise to several published works, being a work of
nanoparticle shape effects, to obtain supramolecular structures on gold electrodes. A paper
pursuing a new strategy for immobilization and the generation of electroactive species (electron
mediator) was published in Electrochimica Acta. The investigation of reaction kinetics of
molecules generated in situ for catalytic effects was performed with a system containing
phthalonitriles. Within this strategy was also performed xanturênico acid immobilization on
carbon nanotubes to check with the reactivity of NADH. Still in the preparation of thin films of
inorganic materials was prepared a film of nickel hydroxide on a glassy carbon electrode, which
showed an interesting catalytic activity for oxidation of sugars. This electrode was used as
detection of carbohydrates in flow systems.
Devices for on site analysis.
An interesting work developed here was the use of roles to perform chromatographic separation
of analytes (in case the ascorbic acid and uric) with electrochemical detection, aiming to
implement the philosophy of "point-of-care-testing". This philosophy seeks to develop low-cost
devices that can perform analysis on site, without the need to transport the sample to the
laboratory for analysis. The work on the use of adsorbent, in this case the chromatographic
paper, with electrodes printed on it has led to publication in Analytical Chemistry. The work was
very innovative and allows a range of options for the development of miniaturized devices in the
low-cost philosophy point-of-care-testing.
FINAL REMARKS
Considering the production obtained in this first year it is possible to verify that the
scientific works was the strongest point in the INCTBio, combined with the number of students
involved in the team. The number of papers published in well reputed journals and the number
of works presented in congresses gives support to the productivity and the quality. The invited
conferences and prizes received by the members of the team suggest that the developed
27
researches by the INCTBio are being well evaluated and recognized by the scientific community.
The transference of knowledge to the companies it is possible to see some initial contacts, which
is normal to expect few interactions with companies at beginning. In relation to the knowledge
dissemination to the society must be given more attention in the next years. The weakest point
was the marketing of the activities developed by the INCTBio, because no action in this
direction was done. In the next year must be carefully treated and some actions to overcome this
problem will be put in practice.
The team will be adjusted joining researchers strategic for the institute, including students
supervised by the INCTBio, thus the number of researchers into the institute will increase and as
a consequence the number of institutions. The collaborations and interactions between the groups
and expand to the other INCT should be given an incentive.
2) PUBLICATIONS LIST
2.1) Published papers
1. A. Sussulini, A. Prado, D.A. Maretto, R.J. Poppi, L. Tasic. C.E.M Banzato, M.A.Z. Arruda,
Metabolic profiling of human blood serum from treated patients with bipolar disorder
employing 1H NMR spectroscopy and chemiometrics. Anal. Chem. 81 (2009) 9755-9763.
2. M.A.Z. Arruda, R.A. Azevedo, Metallomics and chemical speciation: towards a better
understanding of metal-induced stress in plants. Ann. Appl. Biol. 155 (2009) 1-7.
3. M.A.O. Silva, J.S. Garcia, G.H.M.F Souza, M.N. Eberlin, F.C. Gozzo, M.A.Z. Arruda.
Evaluation of sample preparation protocols for proteomic analysis of sunflower leaves.
Talanta 80 (2010) 1545-1551.
4. A.S. Pereira, G. Ferreira, I. Caetano, M.A.U. Martines, P.M. Padilha, A. Santos, G.R. Castro,
Preconcentration and determination of Cu(II) in a fresh water sample using modified silica
gel as a solid phase extraction adsorbent. Journal of Hazardous Materials, 175 (2010) 399403.
5. P.M. Moraes; R.B. Milantonio, G.S. Cagnani, F.A. Santos, C.C.F. Padilha, P.M. Lima, P.M
Padilha. Analytical procedure based on slurry sampling for determining selenium in organic
vegetable samples by graphite furnace atomic absorption spectrometry. European Food
Research and Technology, 229 (2009) 409-414.
6. Saleh, M. A. D.; Neves, R. C. F.; Silva, F. A.; Moraes, P. M.; Loureiro, V. R.; Roldan, P. S.;
Padilha, P.D. GFAAS Determination of Zinc in Fish Feed and Feces Using Slurry Sampling.
Food Analytical Methods, 2 (2009) 162-168.
7. Neves, R. C. F.; Moraes, P. M.; Saleh, M. A. D.; Loureiro, V. R.; Silva, F. A.; Barros, M. M.;
Padilha, C. C. F.; Alvesjorge, S. M.; Padilha P.M. FAAS determination of metal nutrients in
fish feed after ultrasound extraction. Food Chemistry, 113 (2009) 679-683.
28
8. Saleh, M. A. D.; Neves, R. C. F.; Moraes, P. M.; Lima, P. M.; Santos, F. A.; Silva, F. A.;
Padilha, P.M. Iron determinetion by FAAS in fish feed and feces after ultrasound-assisted
extraction. Sensing and Instrumentation for Food Quality and Safety, 3 (2009) 108-113.
9. Moraes, P. M.; Loureiro, V. R.; Neves, R. C. F.; Saleh, M. A. D.; Santos, F. A.; Silva, F. A.;
Padilha, P.M. Determinação de fósforo biodisponível em rações de peixes utilizando extração
assistida por ultra-som e espectrofotometria no visível. Química Nova, 32 (2009) 923-927.
10. Minello, M. C. S.; Paço, A. L.; Martines, M. A. U.; Caetano, L.; Santos, A.; Padilha P.M.;
Castro, G. R. Sediment grain size distribution and heavy metals determination in a dam on the
Paraná river at Ilha Solteira, SP, Brazil.. Journal of Environmental Science and Health. Part
A, Toxic Hazardous Substances and Environmental Engineering, 44 (2009) 861-865.
11. Vasconcelos, B. C.; Bernardes, R. A.; Cruz, S. M. L; Duarte, M. A. H.; Padilha Pm;
Bernardineli, N.; Garcia, R. B.; Bramante, C. M.; Moraes, I. G. Evaluation of pH and calcium
ion release of new root-end filling materials. Oral Surgery, Oral Medicine, Oral
Pathology, Oral Radiology, and Endodontics, 108 (2009) 135-139.
12. J.M.O. Souza, C.R.T. Tarley, Sorbent separation and enrichment method for cobalt ions
determination by graphite furnace atomic absorption spectrometry in water and urine
samples using multiwall carbon nanotubes. Intern. J. Environ. Anal. Chem. 89 (2009) 489502.
13. V. S. Santos, W. J. Santos, L. T. Kubota, C. R. T. Tarley, Speciation of Sb(III) and Sb(V) in
meglumine antimoniate pharmaceutical formulations by PSA using carbon nanotube
electrode. J. Pharm. Biomed. Anal. 50 (2009) 151-157.
14. C. Pereira, A. Kisner, C.R.T, Tarley, N. Duran, L. T. Kubota, Determination of Phenol
Compounds Based on Electrodes with HRP Immobilized on Oxidized Multi-Wall Carbon
Nanotubes. Dyn. Biochem. Process Biotechnol. Mol. Biol., 3 (2009) 75-79.
15. H. Iglesias, L. F. A. Santos, F. C. Gozzo, Identification of cross-linked peptides by highresolution precursor ion scan, Anal Chem. 82 (2010) 909-16.
16. H. Iglesias, L. F. A. Santos, F. C. Gozzo, Collision-induced dissociation of Lys-Lys
intramolecular crosslinked peptides, J Am Soc Mass Spectrom. 20 (2009) 557-66.
17. E. M. M. Flores, A. P. F. Saidelles, J. C. P. Mattos, E. I. Müller, J. S. F. Pereira, J. N. G.
Paniz, V. L. Dressler, Determination of Cd and Pb in Medicinal Plants using Solid Sampling
Flame Atomic Absorption Spectrometry, Intern. J. Environ. Anal. Chem. 89 (2009) 129140.
18. D. Kochhann, A. P. S. Benaduce, C. E. Copatti, K. R. Lorenzatto, M. F. Mesko, E. M. M.
Flores, V. L. Dressler, B. Baldisserotto, Protective effect of high alkalinity against the
deleterious effects of chronic waterborne cadmium exposure on the detection of alarm cues by
juvenile silver catfish (Rhamdia quelen), Arch. Environ. Contam. Toxicol. 56 (2009) 770775.
29
19. Franciscato, F.R. Goulart, N.M. Lovatto, F.A. Duarte, E.M.M. Flores, V.L. Dressler, N.C.
Peixoto, M.E. Pereira, ZnCl2 exposure protects against behavioral and acetylcholinesterase
changes induced by HgCl2. Intern. J. Develop. Neurosci. 27 (2009) 459-468.
20. F.A. Duarte, C.A. Bizzi, F.G. Antes, V.L. Dressler, E.M.M. Flores, Organic, Inorganic and
Total mercury determination in fish by chemical vapor generation with collection on a gold
gauze and electrothermal atomic absorption spectrometry. Spectrochim. Acta Part B. 64
(2009) 513-519.
21. Silva, A.S., L. Hoehne, A.A. Tonin, R.A. Zanette, P. Wolkmer, M.M. Costa, D.P. Moraes,
E.M.M. Flores, J.M. Santurio, S.T. Lopes, S.G. Monteiro, Trypanosoma evansi: levels of
copper, iron and zinc in the bloodstream of infected cats. Exp. Parasitol. 123 (2009) 35-38.
22. Franciscato, T.M. Bueno, L. Moraes-Silva, F.A. Duarte, E.M.M. Flores, V.L. Dressler, M.E.
Pereira, High doses of zinc and copper alter neither cerebral metal levels nor
acetylcholinesterase activity of suckling rats. EXCLI Journal, 8 (2009) 138-147.
23. Kochhann, M.A. Pavanato, S.F. Llesuy, L.M. Correa, A.P.K. Riffel, V.L. Loro, M.F. Mesko,
E.M.M. Flores, V.L. Dressler, B. Baldisserotto, Bioaccumulation and oxidative stress
parameters in silver catfish (Rhamdia quelen) exposed to different thorium concentrations.
Chemosphere 77 (2009) 384-391.
24. A. Alves, I. O. Matos, P. M. Takahashi, E. L. Bastos, H. Martinho, J. G. Ferreira, C. C. Silva,
R. H. A. Santos, A. Paduan-Filho, A.M.D.C. Ferreira, Chloro-Bridged Linear Chain ImineCopper(II) Complex and Its Application as Enzyme-Free Amperometric Biosensor for
Hydrogen Peroxide. Eur. J. Inorg. Chem. 2009 (2009) 2219.
25. W. J. R. Santos, A. R. Sousa, M. P. T. Sotomayor, F. S. Damos, S. M. C. N. Tanaka, L. T.
Kubota, A. A. Tanaka, Manganese Phthalocyanine as a Biomimetic Electrocatalyst for
Phenols in the Development of an Amperometric Sensor, J. Braz. Chem. Soc. 20(2009)11801187.
26. C. De Oliveira, I. B. Valentim, C. A. Silva, E. J. H. Bechara, M. T. S. Tevisan, M. O. F.
Goulart. Fontes vegetais naturais de antioxidantes. Quim. Nova, 32 (2009), 689-702,.
27. P. R. Lima, P. R. B. Miranda, A. B. Oliveira, M. O. F. Goulart, L. T. Kubota. In situ activated
4-nitrophthalonitrile-modified carbon paste electrode for kinetic investigation and for
simultaneous determination of ascorbic and uric acids. Electroanalysis, 21 (2009), 2311 –
2320.
28. D. A. Dos Santos Silva, C. B. Lopes, P. R. Lima, E. de O. Costa, L. T. Kubota, M. O. F.
Goulart. Poly- xanthurenic acid as a new mediator for the electrocatalytic oxidation of
NADH. Electrochem. Commun., (2010) 450–454,
29. M. Vidotti, V.R. Gonçales, B. Danc ; V. S. Quartero , S.I.C. de TORRESI, Platinum
nanoparticles modified electrodes, morphologic and electrochemical studies concerning
electroactive materials deposition. J. Solid State Electrochem., 14 (2010). 675-679.
30
30. M.P. Massafera, S.I.C. de Torresi, Urea Amperometric Biosensors Based on a
Multifunctional Bi-Polymeric Layer: Comparing Enzyme Immobilization Methods. Sens.
Actuat. B,137(2009) 476-482.
31. V.R. Gonçales, T. M Benedetti ; M.P. Massafera, R.M. Torresi, D. G. Moore, S.I.C. de
Torresi . Nanostructured thin films obtained by electrodeposition over a colloidal crystal
template: applications in electrochemical devices. J.Braz. Chem. Soc. 20 (2009) 663-673.
32. M. Vidotti, L.T. Kubota, S.I.C. de Torresi, C.D. Cerri CD ; R.F.Carvalhal, J.C. Dias, R.K.
Mendes. Nickel hydroxide electrodes as amperometric detectors for carbohydrates in flow
injection analysis and liquid chromatography. J. Electroanal. Chem. 636 (2009) 18-23.
33. S. H. Takahashi ; S.I.C.de Torresi, Nitric Oxide sensing by Cytochrome C bonded to a
conducting polymer modified glassy carbon electrode. Synth. Metals, 159(2009) 2159-2161.
34. S. Damos, R. C. S. Luz, A. A. Tanaka, L. T. Kubota, Dissolved oxygen amperometric sensor
based on layer-by-layer assembly using host-guest supramolecular interactions, Anal. Chim.
Acta, 664(2) (2010) 144-150.
35. S. Damos, R. C. S. Luz, A. A. Tanaka, L. T. Kubota, Development of an electroactive layerby-layer assembly based on host–guest supramolecular interactions, J. Electroanal. Chem.,
639 (2010) 36–42.
36. E. Marafon, L.T. Kubota, Y. Gushikem, “FAD-modified SiO2/ZrO2/C ceramic electrode for
electrocatalytic reduction of bromate and iodate”, J. Sol. State Electrochem., 13, 377 (2009)
37. C.M. Maroneze, R.C.S. Luz, R. Landers, Y. Gushikem, “SiO2/TiO2/Sb2O5 /graphite carbon
ceramic conducting material: preparation, characterization, and its use as an
electrochemical sensor”, J. Sol. State Electrochem., 14, 115 (2010)
38. J. Arguello, H.A. Magosso, R.R. Ramos, T.C. Canevari, R.Landers, V.L. Pimentel, Y.
Gushikem, “Structural and electrochemical characterization of a cobalt phthalocyanine bulkmodified SiO2/SnO2 carbon ceramic electrode”, Electrochim. Acta, 54,1948 (2009)
39. H.A. Magosso, R.C. S. Luz, Y. Gushikem, “Preparation and properties of the hybrid
material
n-propyl(3-methylpyridinium)silsesquioxane
chloride.
Application
in
electrochemical determination of nitrite”, Electroanalysis, 22, 216 (2010)
40. H.A. Magosso, N. Fattori, Y.V. Kholin, Y. Gushikem, “Adsorption of metal ions on novel 3n-propyl(methylpyridinium)silsesquioxane chloride polymers surface. Study of heterogeneous
equilibrium at solid-solution interface”, J. Braz. Chem. Soc., 20, 744(2009).
41. Santos, W.J.R.; Lima, P.R.; Tanaka, A.A.; Tanaka, S.M.C.N.; Kubota, L.T.; Determination of
nitrite in food samples by anodic voltammetry using a modified electrode, Food Chem. 113
(4), 1206-1211, (2009).
31
42. Santos; W.J.R., Lima, P.R.; Tarley, C.R.T.; Hoሷehr, N.F.; Kubota, L.T.; Synthesis and
application of a peroxidase-like molecularly imprinted polymer based on hemin for selective
determination of serotonin in blood serum, Anal. Chim. Acta, 63(1), 170-176, (2009).
43. Kisner, A.; Aguiar, M.R.; Vaz, A.F.; Rojas, A.; Cavarsan, F.A.; Diniz, J.A.; Kubota, L.T.;
Submicrometer-MOS capacitor with ultra high capacitance biased by Au nanoelectrodes,
Applied Physics A: Mat. Scie. Proc., 94(4), 831-836, (2009).
44. Kisner, A.; Aguiar, M.R.; Kubota, L.T.; Giant Enhancement of Light Emission from Au
Nanocrystals into a Porous Matrix Integrated with Silicon Platform, J. Nanoscie. Nanotech.,
9, 2592-2597, (2009).
45. Mello, L.D.; Ribeiro, E.S.; Kubota, L.T.; Elmroth, S.K.C.; Pereira, R.M.S.; Electrochemical
and spectroscopic evidences of the interaction between DNA and Pt(II)(dppf)-complex,
Biometals, 22(2), 385-292, (2009).
46. Mendes, R.K.; Carvalhal, R.F.; Stach-Machado, D.R.; Kubota, L.T.; Surface Plasmon
Resonance immunosensor for early diagnosis of Asian rust on soybean leaves, Biosen.
Bioelectron., 24, 2483-2487, (2009).
47. Dias, J.C.; Suzuki, E.; Albuquerque, C.L.; Ferreira, A.L.; Brito, A.R.M.S.; Kubota, L.T.;
Determination of short-chain fatty acids in dietary fiber extract using ion-exclusion
chromatography with suppressed conductivity detection, J. Pharm. Biomed. Anal., 49, 11281132, (2009).
48. Mendes, R.K.; Ferreira, D.C.M.; Carvalhal, R.F.; Peroni, L.A.; Stach-Machado, D.R.; Kubota,
L.T.; Electrochemical development of an immunosensor for detection of Phakopsora
pachyrhizi detection for the early diagnosis of Asian rust on soybean leaves, J. Braz. Chem.
Soc., 20 (4), 795-801, (2009).
49. Tarley, C.R.T.; Santos, V.S.; Baêta, B.E.L.; Pereira, A.C.; Kubota, L.T.; Simultaneous
determination of zinc, cadmium and lead in environmental water samples by potentiometric
stripping analysis (PSA) using multiwalled carbon nanotube electrode, J. Hazard. Mater.,
169 (1-3), 256-262, (2009).
50. Santhiago, M.; Lima, P.R.; Santos, W.J.R.; Oliveira, A.B.; Kubota, L.T.; In situ activated 3,5dinitrobenzoic acid covalently attached to nanostructured platform for NADH
electrooxidation, Electrochim. Acta, 54(26), 6609-6616, (2009).
51. Pereira, A.C.; Kisner, A.; Duran, N.; Kubota, L.T.; The Effects of Dimensionality on
Electrochemical Sensors Based on Carbon Nanotubes and Metallic Nanowires, J. Nanoscie.
Nanotechnol., 10(2) (2010) 651-667.
52. Santhiago, M.; Lima, P.R.; Santos, W.J.R.; Kubota, L.T.; An amperometric sensor for lcysteine based on nanostructured platform modified with 5,5_-dithiobis-2-nitrobenzoic acid
(DTNB), Sen. Actuat. B, 146 (2010) 213-220.
32
53. Carvalhal, R.F.; Kfouri, M.S.; Piazetta, M.H.O.; Gobbi, A.L.; Kubota, L.T.; Electrochemical
detection in a paper based separation device, Anal. Chem., 82, 1162-1165, (2010).
54. A.M.P. Braga, M.A. Silva, M.P. Pedroso, F. Augusto, L.E.S. Barata, Volatile composition
changes of pineapple during drying in modified and controlled atmosphere, Int. J. Food Eng.
6 (2010) 12-12.
55. F. Augusto, E. Carasek, R.G.C. Silva, S.R. Rivellino, A.D. Batista, E. Martendal, New
sorbents for extraction and microextraction techniques, J. Chromatogr. A 1217 (2010)
2533-2542.
56. A.M.P. Braga, M.P. Pedroso, F. Augusto, M.A. Silva, volatiles identification in pineapple
submitted to drying in an ethanolic atmosphere; Dry. Technol. 27 (2009) 248-257.
57. M.G. Pizzolatti, C. Soldi, B.G. Mendes, J.H. Bortoluzzi, E. Carasek, F. Missau, analysis of
volatile compounds released from flowers and roots of polygala cyparissias and polygala
paniculata by headspcace/SPME, J. Essent. Oil Res. 21 (2009) 255-258.
58. E. P. Moraes, F. G. Tonin, L. G. Dias, J. P. S. Farah, M. F. M. Tavares, Assessment of solutemicelle interactions in electrokinetic chromatography using quantitative structure retention
relationships; In Chemometrics Methods In Capillary Electrophoresis, Grady Hanrahan, G.
And Frank A. Gomez, Eds.; Chapter 15, 345-366, John Wiley & Sons, New York, 2010, Isbn
978-0-470-39329-1
59. R. G. Peres, E. P. Moraes, G. A. Micke, F. G. Tonin, M. F. M. Tavares, D. B. RodriguezAmaya, Rapid method for the determination of organic acids in wine by capillary
electrophoresis with indirect UV detection, Food Control 20(2009)548-552.
60. M. L. L. Moraes, S. L. Moraes, E. A. Pereira, M. F. M. Tavares, Estratégias de préconcentração em eletroforese capilar (ce). parte i. manipulação da velocidade eletroforética
do analito; Quim. Nova 32(2009)1041-1046.
61. J. L. Costa, F. G. Tonin, L. A. Zanolli Filho, A. A. M. Chasin, M. F. M. Tavares, Simple
method for determination of cocaine and main metabolites in urine by ce coupled to ms,
Electrophoresis 30(2009)2238-2244.
62. R. G. Peres, G. A. Micke, M. F. M. Tavares, D. B. Rodriguez-Amaya, Multivariant
optimization, validation, and application of capillary electrophoresis for simultaneous
determination of polyphenols and phenolic acids in wines, J. Sep. Sci. 32(2009)3822-3828.
63. T. S. Fukuji, F. G. Tonin, M. F. M. Tavares, Optimization of a method for determination of
phenolic acids in exotic fruits by capillary electrophoresis, J. Pharm. Biom. Anal.
51(2010)430-438.
33
64. M. L. L. Moraes, M. F. M. Tavares, E. A. Pereira, Estratégias de pré-concentração em
eletroforese capilar. parte ii: manipulação da velocidade da fase dispersa/secundária, Quim
Nova 33(2010)466-470.
65. J.A. Fracassi Da Silva, N.V. Castro, D.P. Jesus, A.F. Faria, M.V.N. Souza, M.A.L. Oliveira,
Fast determination of ethambutol in pharmaceutical formulations using capillary
electrophoresis with capacitively coupled contactless conductivity detection, Electrophoresis
31( 2010) 570-574.
66. C. Guimarâes, J. A. F. Da Silva, D. P. Jesus, Comparison of potassium and sodium content in
diet and non-diet soft drinks by using capillary electrophoresis with capacitively coupled
contactless conductivity detection, Eclética Química 34(2009) 51-56.
67. C. Guimarâes, C. C. Rezende, J. A. F. Da Silva, D. P. Jesus, Simultaneous determination of
free fluoride and monofluorophosphate in toothpaste by capillary electrophoresis with
capacitively coupled contactless conductivity detection. Talanta 78(2009) 1436-1439.
68. G. R. M. Duarte, C. W. Price, J. L. Littlewood, D. M. Haverstick, J. P. Ferrance, E. Carrilho
and J. P. Landers, Characterization of dynamic solid phase dna extraction from blood with
magnetically controlled silica bead, Analyst. 135(2010) 531 – 537.
69. M. V. C. Frankenfeld, W. K. T. Coltro, E. Carrilho, D. Diamond And S. M. Lunte, Dual
contactless conductivity and amperometric detection on hybrid pdms/glass electrophoresis
Microchips, Analyst.135, 2010, 96 – 103
70. A.V. C. Simionato, E. Carrilho, M. F. M. Tavares, CE-MS and related techniques as a
valuable tool in tumor biomarkers research, Electrophoresis. 31(2010)1214 – 1226.
71. V. C. Simionato, M. E. Silvastenico, S. M. Tsai, E. Carillho, Evidences of siderophores
synthesis by grapevine xylella fastidiosa, causal agent of pierce s disease, through
instrumental approaches. J. Braz. Chem. Soc. 21(2010)635 – 641.
72. R. F. Travensolo, M. V. C. G. Costa, L. M. Carareto-Alves, E. Carrilho, E. G. M. Lemos,
Production of dna microarray and expression analysis of genes from xylella fastidiosa in
different culture media, Braz. Arch. Biol. Tech. 52(2009)555 – 566.
73. B. Eguiluz, G. R. Salazar-Banda, M. E. Funes-Huacca, J. V. Alberice, E. Carrilho, S. A. S.
Machado And L. A. Avaca, Sequence-specific electrochemical detection of alicyclobacillus
acidoterrestris dna using electroconductive polymer-modified fluorine tin oxide electrodes,
Analyst. 134(2009)314 – 319.
74. S. Caruso, R. Travenzolo, R.C. Bicudo, E. G. M. Lemos, A. P. U. Araujo, E. Carrilho, 'Alfa'hydroxynitrile lyase protein from xylella fastidiosa : cloning, expression, and
characterization. Microb. Pathogenesis. 47(2009)118 – 127.
34
2.2) Accepted/submitted papers
1. M.A.O. Silva, A. Sussulini, M.A.Z. Arruda, Metallomics as an interdisciplinary area
involving proteins and metals, Expert Rev. Proteomics, no prelo.
2. A.R. Brandão, H.S. Barbosa, M.A.Z. Arruda, Image analysis of two-dimensional gel
electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds,
J. Proteomics, no prelo.
3. C. R. Ferreira, S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F.
Santos, E. G. Lo Turco, J. H. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M.
Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, M. N. Eberlin, Single embryo and
oocyte lipid fingerprinting by mass spectrometry, J Lipid Res. 2010, aceito para publicação.
4. Sussulini, H. Dihazi, C.E.M Banzato, M.A.Z. Arruda, W. Sühmer, H. Ehrenreich, O. Jahn, H.
Kratzin, Proteome profiling indentifies potential biomarkers for bipolar disorder and its
treatment with lithium, Biol. Psychiatry, submetido.
5. Sussulini, J.S. Becker, H. Kratzin, C.E.M Banzato, M.A.Z. Arruda, Metallomics studies of
human blood serum from treated patients with bipolar disorder, Anal. Chem., submetido.
6. R. A. Hauser-Davis, R. C. Campos, R. L. Ziolli Use of fish protein biomarkers in situations of
environmental impact: a review, Metallomics, submetido.
7. C. Costa Júnior, A. S. Luna, M. A. Vieira, R. C. Campos Determination of platinum, cisplatin
and carboplatin in human urine by atomic absorption spectrometric methods, Analytical
Biochemistry, submetido.
8. P. Valderrama, R. J. Poppi, Second order standard addition method and fluorescence
spectroscopy in the quantification of ibuprofen enantiomers in biological fluids, Chemom.
Intell. Lab. Syst., aceito para publicação.
9. D. Bernardes, R. J. Poppi, Marcelo M. Sena, Direct determination of trans-resveratrol in
human plasma by spectrofluorimetry and second order standard addition, Talanta, aceito
para publicação.
O. Matos, T. L. Ferreira, T. R. L. C. Paixão, A. S. Lima, M. Bertotti, W. A. Alves,
10.
Approaches for Multicopper Oxidase in the Design of Novel Electrochemical Sensors for
Analytical Applications, Electrochim. Acta (2010).
11.
W. A. Alves, A. C. Sant´ana, M. P. Abbott, P. H. Mello, H. Martinho, R. H. A. Santos, J.
G. Ferreira, M. L. A. Temperini, A. Paduan-Filho, A. M. D. C. Ferreira, Dinuclear AzideBridged Copper(II) Complex as Building Block for the Assembly of a 2D-Supramolecular
Array, Sci. Adv. Mater. (2010).
35
12.
T. C. Cipriano, P. M. Takahashi, D. de Lima, V. X. de Oliveira Jr., J. A. Souza, H.
Martinho, W. A. Alves, Spatial Organization of Peptide Nanotubes for Electrochemical
Devices, J. Mater. Sci. (2010).
13.
P. Sousa, A. S. Polo, R. M. Torresi, S. I. Córdoba de Torresi, W. A. Alves, Chemical
modification of a nanocrystalline TiO2 film for efficient electric connection of glucose
oxidase, J. Coll. Interf. Sci. (2010)
14.
C. Boni, M. P. T. Sotomayor, M. R. V. Lanza, S. M. C. N. Tanaka, A. A. Tanaka,
Application of a Biomimetic Sensor Based on Iron Phthalocyanine Chloride: 4–
Methylbenzylidene–Camphor Detection, J. Braz. Chem. Soc.;(2010)
15.
Morais, F.L. Pissetti, A.M.S. Lucho, Y. Gushikem, “Influence of copper
hexacyanoferrate film thickness on the electrochemical properties of self-assembled 3mercaptopropyl gold electrode and application as a hydrazine sensor”, J. Solid State
Electrochem. (2010)
16.
R.F. Carvalhal, D.S. Machado, R.K. Mendes, A.L.J. Almeida, N.H. Moreira, M.H.O.
Piazetta, A.L. Gobbi, L.T. Kubota, Development of a disposable amperometric biosensor for
salicylate based on a plastic electrochemical microcell, Biosens. Bioelectron. (2010)
17.
M. A. Oliveira, C. A. Silva, A. M. Silva, M. F. M. Tavares, M. J. Kato, Development and
validation of micellar electrokinetic chromatography (MEKC) method for the quantitative
determination of butenolides in Piper malacophyllum, Phytochem. Anal., submitted.
18.
Bechara, J. Massari, R. Tokikawa, L. A. Zanolli Filho, M. F. M. Tavares, N. Assunção,
Methylglyoxal Reacts with Peroxynitrite Yielding Acetyl Radical and Promoting L-Lysine
Acetylation, J. Am. Chem. Soc., submitted.
19.
R. G. C. Silva, C. R. M. Vigna, C. B. G. Bottoli, C. H. Collins, F. Augusto, Molecularly
imprinted silica as a selective SPE sorbent for triazine herbicides, J. Sep. Sci. DOI:
10.1002/jssc.200900785.
20.
M. Borges, C. B. G. Bottoli, C. H. Collins, Possibilidades e limitações no uso da
temperatura em cromatografia líquida de fase reversa, Quim. Nova, no prelo,
http://quimicanova.sbq.org.br/qn/No%20Prelo/RV/RV09651.pdf
21.
W.K.T. Coltro, D.P. Jesus, J.A. Fracassi da Silva, C.L. Lago, E. Carrilho, Toner and
Paper-based Fabrication Techniques for Microfluidic Applications, Electrophoresis (aceito).
22.
A.B. Bergamo, J.A. Fracassi da Silva, D.P. Jesus, Simultaneous Determination of
Aspartame, Cyclamate, Saccharin and Acesulfame-K in Soft Drinks and Tabletop Sweetener
Formulations by Capillary Electrophoresis with Capacitively Coupled Contactless
Conductivity Detection, Food Chemistry (submetido)
36
3) Presented works in Congress
1. A. Sussulini, H. Dihazi, C.E.M. Banzato, M.A.Z. Arruda, W. Stühmer, H. Ehrenreich, O.
Jahn, H. Kratzin. Protein profiling by mass spectrometry analyses identify blood serum
candidate biomarkers for bipolar disorder and the treatment using lithium. 18th International
Mass Spectrometry Conference, 30 de agosto a 04 de setembro de 2009, Bremen, Alemanha.
(apresentação como painel)
2. M.A.O. da Silva, A.R. Brandão, S.A.L. Andrade, P. Mazzafera, M. A.Z. Arruda, Influence of
different zinc and selenium levels in the sunflower plants development. Colloquium
Spectroscopicum Internationale XXXVI, 30 de agosto a 03 de setembro de 2009, Budapeste,
Hungria. (apresentação como painel)
3. L.R.V. Mataveli, M.A.Z. Arruda, Comparative metallomics of transgenic and non-transgenic
soybeans using HPLC-ICP-MS. Colloquium Spectroscopicum Internationale XXXVI, 30 de
agosto a 03 de setembro de 2009, Budapeste, Hungria. (apresentação como painel)
4. M.A.Z. Arruda, Metallomics as integrated biometal science. Palestra proferida no 7th
International Congress of Pharmaceutical Sciences – CIFARP 2009, Ribeirão Preto, SP, em
07 de Setembro de 2009.
5. M.A.Z. Arruda, Metalômica. Palestra proferida no Congresso Analítica Latin America, São
Paulo, SP, 09 de Setembro de 2009.
6. R.A.O. Silva, M.A.Z. Arruda, Otimização da extração de transferrina empregando ponto
nuvem. XVII Congresso Interno de Iniciação Cientifica da Unicamp, 23 a 24 de setembro de
2009, Campinas, São Paulo. (apresentação como painel)
7. M.A.Z. Arruda, A. Sussulini, L. Tasic, C.E.M. Banzato, H.S. Barbosa, A.R. Brandão,
Biomarkers and comparative “omics”. 1st International Congress on Analytical Proteomics,
30 de setembro a 03 de outubro de 2009, Costa da Caparica, Lisboa, Portugal. (apresentação
oral)
8. H.S. Barbosa, A.R. Brandão, M.A.Z. Arruda, Comparative proteomic study involving
transgenic and non-transgenic soybean seeds. 1st International Congress on Analytical
Proteomics, 30 de setembro a 03 de outubro de 2009, Costa da Caparica, Lisboa, Portugal.
(apresentação como painel)
9. A.Sussulini, C.E.M Banzato, M.A.Z Arruda, Avaliação do perfil ionômico para o transtorno
afetivo bipolar e seu tratamento com lítio em amostras de soro sangüíneo. 15º Encontro
Nacional de Química Analítica e 3º Congresso Iberoamericano de Química Analítica, 18 a 21
de outubro de 2009, Salvador, Bahia (apresentação oral).
10. M.A.Z. Arruda, Metalômica como ciência integrada dos biometais. Palestra no âmbito do VII
Ciclo de Palestras e Filmes Científicos do Departamento de Química, Faculdade de Filosofia,
Ciências e Letras de Ribeirão Preto, USP, Ribeirão Preto, SP, em 09 de Novembro de 2009.
37
11. M.A.Z. Arruda, Metalômica e ICP-MS: um casamento perfeito. Palestra no IX Encontro de
usuários de ICP-MS. Faculdade de Geologia, Universidade Estadual do Rio de Janeiro –
UERJ, Rio de Janeiro, RJ, em 02 de Dezembro de 2009.
12. A.Sussulini, J.S. Becker, C.E.M. Banzato, M.A.Z. Arruda. LA-ICP-MS for the detection of
human blood serum metalloproteins in 2-D electrophoresis gels. 3º Congresso da Sociedade
Brasileira de Espectrometria de Massas, 12 a 15 de dezembro de 2009, Campinas, São Paulo.
(apresentação painel)
13. M.A.O da Silva, M.A.Z. Arruda, Inorganic mass spectrometry as a fundamental tool for
establishing interactions between selenium and sulfur in the sunflower metabolism. 3º
Congresso da Sociedade Brasileira de Espectrometria de Massas, 12 a 15 de dezembro de
2009, Campinas, São Paulo. (apresentação painel)
14. M.A.Z. Arruda, Metallomics and mass spectrometry: the perfect couple. Palestra no 3o
Congresso Brasileiro de Espectrometria de Massas – BrMASS, Campinas, SP, em 15 de
Dezembro de 2009.
15. M.A.Z. Arruda, Metaloproteômica e espectrometria atômica: de mera rima a um casal
perfeito. V Workshop sobre Espectrometria Atômica, PUC-Rio, Rio de Janeiro, em 02 de
Fevereiro de 2010.
16. Silva, F.A.; Neves, R.C.F.; Lima, P.M.; Santos, F. A.; Silva, M.A.O.; Machado, A.U.;
Padilha, P.M. Cálcio em proteínas de plasma e tecido hepático de tilápia do Nilo
(Oreocromis niloticus) separadas por eletroforese em segunda dimensão. In: 46a Reunião
Anual da Sociedade Brasileira de Zootecnia, 14 a 17 de julho de 2009, Maringá-PR.
17. Lima, P.M.; Neves, R.C.F. ; Silva, F.A.; Saleh, M.A.D.; Santos, F.A.; Silva, M.A.O.; Padilha,
P.M. Avaliação de Zn ligado à proteinas de plasma e tecido hepático de tilápia do Nilo
(Oreochromis niloticus) separadas por 2-D-PAGE. In: 46 ª Reunião Anual da Sociedade
Brasileira de Zootecnia, 14 a 17 de julho de 2009, Maringá-PR.
18. Carvalho, T.S.; Machado, A.U.; Cavecci, B.; Garcia, L.A; Silva, F.A.; Neves, R.C. F.; Lima,
P.M.; Santos, F. A.; Padilha, P. M.. Mapeamento em spots protéicos de amostras de tecido
hepático de tilápia do Nilo (Oreochromis Niloticus). In: Congresso Brasileiro de Química CBQ, 13 a 17 de outubro de 2009, Porto Alegre – RS.
19. Machado, A.U.; Carvalho, T.S.; Cavecci, B.; Garcia, L.A.; Silva, F.A.; Neves, R.C. F.; Lima,
P.M.; Santos, F.A.; Padilha, P.M. Fracionamento de Protéina de Plasma de Tilápia do Nilo
(Oreochromi Niloticus) por Eletroforese Bidimensional. In: XLIX Congresso Brasileiro de
Química – CBQ, 13 a 17 de outubro de 2009, Porto Alegre – RS.
20. Silva, F.A.; Neves, R.C.F.; Lima, P.M.; Santos, F.A.; Saleh, M.A.D.; Carvalho, T.S.; Padilha,
P.M. Ferro em proteínas de plasma, tecido muscular e hepático de tilápia do Nilo
(Oreochromis Niloticus) separadas por eletroforese em segunda dimensão. In: Sociedade
Brasileira de Zootecnia - SBZ, 14 a 17 de julho de 2009, Maringá-PR.
38
21. Neves, R.C.F.; Silva, F.A.; Silva, M.A.O.; Santos, F.A.; Lima, P.M.; Arruda, M.A.; Padilha,
P.M. Mapeamento de cálcio, ferro e zinco por SR-XRF em spots protéicos de tecido hepático
de tilápia do Nilo (Oreochromis niloticus). In: 3º Simpósio de Nutrição e Saúde de peixes, 4 a
6 de novembro de 2009, Botucatu SP. 4 a 6 de novembro de 2009, Botucatu SP.
22. Lima, P.M. ; Santos, F.A. ; Neves, R.C.F. ; Machado, A.U. ; Carvalho, T.S. ; Loureiro, V.R. ;
Moraes, P.M. ; Padilha, P.M. . Otimização de procedimentos analíticos para fracionamento
de proteínas de amostras de plasma de tilápia do Nilo utilizando 2D PAGE. In: 3º Simpósio
de Nutrição e Saúde de peixes, 4 a 6 de novembro de 2009, Botucatu SP.
23. Santos, F.A.; Lima, P.M.; Neves, R.C.F.; Carvalho, T.S.; Machado, A.U.; Moraes, P.M.;
Loureiro, V.R; Padilha, P.M. Fracionamento de zinco em amostras de tecido hepático de
tilápia do Nilo (Oreochromis niloticus) utilizando 2D PAGE e SR-XRF. In: 3º Simpósio de
Nutrição e Saúde de peixes, 4 a 6 de novembro de 2009, Botucatu SP.
24. Padilha, P.M., Arruda, M.A.Z., Silva, F.A., Neves, R.C.F., Silva, M.A.O., Lima, P.M., Saleh,
M.A.D. Manganese mapping by protein spots of plasma and of liver and muscle tissues of
Nile tilapia (Oreochromis niloticus) In: EUROANALYSIS, 4 a 13 de setembro de 2009,
Innsbruck,Austria.
25. Neves, R.C.F.; Silva, F.A.; Da Silva, M.A.O.; Saleh,M.A.D., Santos, F.A.; Lima, P.M.;
Arruda, M.A.Z.; Padilha P.M. Cobre em proteínas de plasma e tecidos muscular e hepático
de tilápia do Nilo (Oreochromis niloticus) separadas por 2D PAGE. In: 32o Reunião Anual
da Sociedade Brasileira de Química, 30 de maio a 02 de junho de 2009, Fortaleza/CE.
26. Neves, R.C.F.; Silva, F.A.; Marcelo, A.O Da Silava; Santos, F.A.; Lima, P.M.; Arruda,
M.A.Z.; Padilha, P.M. Mapeamento de cálcio, ferro e zinco por SR-XRF em spots protéicos
de tecido hepático de tilápia do Nilo (Oreochromi Niloticus). In: 15o Encontro Nacional de
Química Analítica e 3o Congresso Iberoamericano de Química Analítica – ENQA, 18 a 21 de
Outubro 2009, Salvador/BA. Apresentação na forma oral.
27. V.S. Santos, P.R. Lima. W.J. Santos, L.T. Kubota, C.R.T. Tarley, Emprego de eletrodo de
pasta de nanotubo de carbono para determinação de Antimônio(III) por redissolução
potenciométrica. XVII SIBEE, 19 a 23 de abril de 2009, Fortaleza, CE. Apresentação na
forma oral.
28. W.J. Santos, P.R. Lima, C.R.T. Tarley, L.T. Kubota, Estudo cinético de polímeros
biomiméticos a peroxidase com impressão molecular de substratos fenólicos. XVII SIBEE,
19 a 23 de abril de 2009, Fortaleza, CE. Apresentação na forma de painel.
29. T.C. Ávila, L.R. Sartori, L.T. Kubota, C.R.T. Tarley, Emprego de Reator Enzimático Baseado
em Polímeros Molecularmente Impressos para Oxidação Catalítica de Epinefrina em Sistema
FIA. 32a Reunião Anual da SBQ, 30 de maio a 02 de junho de 2009, Fortaleza, CE.
Apresentação na forma de painel.
30. T.C. Ávila, M. G. Segatelli, C.R.T. Tarley, Avaliação do Desempenho Seletivo de Sílica Gel
Organicamente Modificada e Ionicamente Impressa com Íons Cu2+ Empregando Sistema
39
FIA. 32a Reunião Anual da SBQ, 30 de maio a 02 de junho de 2009, Fortaleza, CE.
Apresentação na forma de painel.
31. F.F. Fernandes, M.G. Segatelli, C.R.T. Tarley, Extração Seletiva em Linha de Íons Co2+
Empregando Sílica Gel Funcionalizada e com Impressão Iônica Bidimensional. 32a Reunião
Anual da SBQ, 30 de maio a 02 de junho de 2009, Fortaleza, CE. Apresentação na forma de
painel.
32. L.R. Nacano, M.G. Segatelli, C.R.T. Tarley, Determinação de Ni (II) por Espectrometria de
Absorção Atômica após etapa de Pré-concentração em Polímeros Impressos
Hierarquicamente. Jornada Científica da Unifal-MG, 24 a 26 de setembro de 2009, Alfenas,
MG. Apresentação na forma de painel.
33. V.S. Santos, C.R.T. Tarley, Desenvolvimento de um método eletroanalítico para
determinação de Sb (III) por potenciometria de resissolução empregando eletrodo de pasta
de nanotubos de carbono. Jornada Científica da Unifal-MG, 24 a 26 de setembro de 2009,
Alfenas, MG. Apresentação na forma oral.
34. F.N. Andrade, H.H.V. Costa, M.G. Segatelli, C.R.T. Tarley, Emprego de Polímero Híbrido
Orgânico-Inorgânico Impresso Ionicamente para Pré-concentração Seletiva de Pb2+ e
Determinação por TS-FF-AAS. Jornada Científica da Unifal-MG, 24 a 26 de setembro de
2009, Alfenas, MG. Apresentação na forma de painel.
35. T.C. Ávila, M.G. Segatelli, C.R.T. Tarley, Sistema de Pré-concentração em Fluxo de Íons
Cu2+Empregando Sílica Gel com Impressão Iônica. 32a Reunião Anual da SBQ, 30 de maio a
02 de junho de 2009, Fortaleza, CE. Apresentação na forma de painel.
36. A.F. Gomes, F.C Gozzo, ESI- and MALDI-MS/MS Analysis of Intramolecular Cross-linked
Peptides with a Photoactivated NHS-Diazirine Cross-linker. 57th ASMS Conference on Mass
Spectrometry, 2009, 31 maio – 4 junho, Philadelphia, EUA.. Apresentação na forma de
pôster.
37. L.F.A. Santos, A.H. Iglesias, F.C. Gozzo, Fragmentation of Intermolecular Cross-Linked
Peptides by ECD and IRMPD, 57th ASMS Conference on Mass Spectrometry, 2009. 31 maio
– 4 junho, Philadelphia, EUA.Apresentação na forma de pôster.
38. A.R. Figueiredo, F.C. Gozzo, Evaluation of Cross-linker and Protein Dynamics in Crosslinking Coupled to Mass Spectrometry Experiments, 57th ASMS Conference on Mass
Spectrometry, 2009. 31 maio – 4 junho, Philadelphia, EUA. Apresentação na forma de pôster.
39. E.J. Pilau, F.C. Gozzo, Protein Footprinting by Peroxide Photolysis using Hg Lamp and 213
nm Laser as Radiation Sources, 57th ASMS Conference on Mass Spectrometry, 2009. 31
maio – 4 junho, Philadelphia, EUA.Apresentação na forma de pôster.
40. F.C. Gozzo, A.H. Iglesias, L.F.A. Santos, Use of marker ions and High Resolution Precursor
Ion Scan for the Identification of Cross-linked Peptides. 57th ASMS Conference on Mass
40
Spectrometry, 2009., 31 maio – 4 junho, Philadelphia, EUA. Apresentação na forma de
pôster.
41. C.R. Ferreira, R.R. Catarino, J.S. Garcia, G.B. Sanvido, F.C. Gozzo, R.P. Bertolla, M.N.
Eberlin, Single Oocyte and Embryo Mass Spectrometry, 18th International Mass Spectrometry
Conference, 2009, Bremen, Alemanha, 30 agosto-4 Setembro. Apresentação na forma de
pôster.
42. F.C. Gozzo, Identification of Cross-Linked Peptides by High Resolution Precursor Ion Scan,
3o BrMASS, Campinas, SP, 12-15 Dezembro 2009, Apresentação na forma oral.
43. L.F.A. Santos, A.H. Iglesias, E.J. Pilau, A.F. Gomes, F.C. Gozzo. Ion Mobility Separation of
Isomeric Modified Peptides Present in Cross-Linking Experiments, 3o BrMASS, Campinas,
12-15 Dezembro 2009. Apresentação na forma de pôster.
44. E.J. Pilau, A.F. Gomes, M.A.O. da Silva, M.A.Z. Arruda, F.C. Gozzo Protein Footprinting by
Peroxide Photolysis using Hg Lamp and 213 nm Laser as Radiation Sources, 3o BrMASS,
Campinas, 12-15 Dezembro 2009. Apresentação na forma de pôster.
45. A.R. Figueiredo ; P.C.T. Souza, M.S. Skaf, F.C. Gozzo Evaluation of Cross-linker and
Protein Dynamics in Cross-linking Coupled to Mass Spectrometry Experiments., 3o BrMASS,
Campinas, 12-15 Dezembro 2009. Apresentação na forma de pôster.
46. A.F. Gomes, F.C. Gozzo Analysis of Cross-linked Proteins by Traveling-Wave Ion Mobility
Mass Spectrometry, 3o BrMASS, Campinas, 12-15 Dezembro 2009. Apresentação na forma
de pôster.
47. E.M.M. Flores, D.P. Moraes, J.S.F. Pereira, P.A. Mello, J.N.G. Paniz, G. Knapp, Sample
Preparation Based on Microwave Induced Combustion for Further Determination of
Halogens in Elastomers. XXXVI Colloquium Spectroscopicum Internationale, 30 de agosto a
03 de setembro de 2009, Budapeste, Hungria. Apresentação na forma de poster.
48. F.A. Duarte, C.M. Moreira, J.N. G. Paniz, E. M.M. Flores, V.L. Dressler, Arsenic speciation
by LC-ICP-MS in white wine produced in South America. XXXVI Colloquium
Spectroscopicum Internationale, 30 de agosto a 03de setembro de 2009, Budapeste, Hungria.
Apresentação na forma de poster.
49. C.A. Bizzi, J.S. Barin, F.G. Antes, E.M.M. Flores, J.A. Nóbrega - Influência da pressão de O2
sobre a eficiência de decomposição de fígado bovino em forno de microondas com ácido
diluído. 15o. Encontro Nacional de Química Analítica – ENQA, 18 a 21 de Outubro de 2009,
Salvador, BA. Apresentação na forma de poster.
50. M.M. Mesko, E.M. Saraiva, L.R. Ferreira, V.A. Pereira, C.A. Bizzi, P.A. Mello, V.L.
Dressler, E.M.M. Flores - Iodine determination in food by ICP-MS after digestion by MIC
(Friday Session), 2010 Winter Conference on Plasma Spectrochemistry, 04 a 09 de janeiro de
2010, Fort Myers, FL, USA.
41
51. E.M.M. Flores, V.L. Dressler, M.M. Mesko, F.G. Antes, Mini-curso Preparo de Amostras
para Determinação de Elementos traços, ministrado no 15o. Encontro Nacional de Química
Analítica – ENQA, 18 a 21 de Outubro de 2009, Salvador, BA. Apresentação de Mini-curso.
52. Hauser-Davis ; Gonçalves, R.A. ; Fernandez, W.L.S. ; Campos, R.C. ; Ziolli, R.L. . Possible
influence of metals on piscine morphological biomarkers. 1st Ibero-American Meeting On
Toxicology And Environmental Health, 2009.
53. P. Valderrama, R. J. Poppi, Second order standard adittion method and fluorescence
spectroscopy in the quantification of ibuprofen in biological fluids, CHIMIOMETRIE 2009,
30 de novembro a 01 de dezembro de 2009, Paris – França. Apresentação na forma de pôster.
54. P. Valderrama, R. R. Poppi, Determinação de enantiômeros do ibuprofeno em plasma e urina
por espectrofluorimetria e método adição de padrão de segunda ordem. 32ª Reunião Anual
da Sociedade Brasileira de Química – SBQ, 30 de Maio a 02 de Junho de 2009, Fortaleza, CE.
Apresentação na forma de pôster.
55. A.D. Bernardes, R. J. Poppi, Marcelo M. Sena, Estimativa do pKa do trans-resveratrol
usando espectrofluorimetria e PARAFAC. 15º Encontro Nacional de Química Analítica –
ENQA, 18 a 21 de Outubro de 2009, Salvador, BA. Apresentação na forma de poster.
56. D. Bernardes, R. J. Poppi, Marcelo M. Sena, Determinação Direta de trans-Resveratrol em
Plasma Humano usando Espectrofluorimetria e Adição-Padrão de Segunda Ordem. 32ª
Reunião Anual da Sociedade Brasileira de Química – SBQ, 30 de Maio a 02 de Junho de
2009, Fortaleza, CE. Apresentação na forma de poster.
57. P.R. Lima, W.J.R. Santos, R.C.S. Luz, F.S. Damos, A.B. Oliveira, M. O. F.Goulart, L.T
Kubota. Sensor amperométrico a base de camadas alternadas de FeT4MPyP e CuTSPc para
determinação de 4-nitrofenol em níveis nanomolar. XVI Simpósio Brasileiro de
Eletroquímica e Eletroanalítica – XVI SIBEE. Águas de Lindóia, São Paulo, Brasil.
58. P.R. Lima, W.J.R. Santos, R.C.S. Luz, F.S. Damos, A.B. Oliveira, M. O. F.Goulart, L.T
Kubota Sensor amperométrico a base de pasta de carbono modificada com 4-nitroftalonitrila
para quantificação de cisteína (CySH). XVI Simpósio Brasileiro de Eletroquímica e
Eletroanalítica – XVI SIBEE. Águas de Lindóia, São Paulo, Brasil.
59. P.R. Lima, W.J.R. Santos, R.C.S. Luz, F.S. Damos, A.B. Oliveira, M. O. F.Goulart, L.T
Kubota Determinação amperométrica de L-glutationa (GSH) com eletrodo de pasta de
carbono modificada com 4-nitroftalonitrila. XVI Simpósio Brasileiro de Eletroquímica e
Eletroanalítica – XVI SIBEE. Águas de Lindóia, São Paulo, Brasil.
60. P.R. Lima, W.J.R Santos, M. Santhiago, A.B. Oliveira, D. Ernsthauser, M.O.F. Goulart, L. T.
Kubota Configuração de uma plataforma a base de nanotubos de carbono com TCNP para
oxidação eletrocatalítica de NADH. XVII Simpósio Brasileiro de Eletroquímica e
Eletroanalítica – XVII SIBEE. Fortaleza, Ceará, Brasil.
42
61. P. R. Lima, J. R. M. Reys, A. G. Cioletti, A. S. Ribeiro, M. O. F. Goulart, L. T. Kubota.
Sensor amperométrico a base de hemina imobilizada em sílica gel modificada com óxido de
titânio para determinação eletrocatalítica de artemisinina. XVII Simpósio Brasileiro de
Eletroquímica e Eletroanalítica – XVII SIBEE. Fortaleza, Ceará, Brasil.
62. M. O. F. Goulart, F. Silva, C. Lopes, P. R. Lima, L.T. Kubota. Xanthurenic Acid: a New
Mediator for the Electroanalysis of NADH. 60th Annual Meeting of the International Society
of Electrochemistry - 60th ISE. Beijing, China.
63. F. W. A. Barros, D. P. Bezerra, P. M. P. Ferreira, B. C. Cavalcanti, T. G. Silva, M. C. A.
Lima, S. L. Galdina, I. R. Pitta, M. A. B. F. Moura, F. C. Abreu, M. O. F. Goulart, L. V.
Costa-Lotufo, M. O. Moraes, C. Pessoa. Cytotoxic Activity of New Acridine Compounds In
Cancer Cell Lines. Hawai., EUA.
64. P. R. Lima, W. J. R. Santos, M. O. F. Goulart, L. T. Kubota. Preparation of a Molecularly
Imprinted Catalyst and its Application for Amperometric Sensor. ICAM 2009, setembro
2009, RJ, Brasil. Apresentação na forma oral.
65. M. O. F. Goulart, F. Silva, C. Lopes, P. R. Lima, L.T. Kubota - A new chemical sensor for
NADH based on multi-walled carbon nanotubes and xanthurenic acid. ICAM 2009, setembro
2009, RJ, Brasil. Apresentação na forma de poster.
66. M. O. F. Goulart, F. Silva, C. B. Lopes, P. R. Lima, L.T. Kubota. I584 - New nanostructured
platforms based on niclosamide immobilized on multi-wall carbon-nanotubes for
electrocatalytic NADH oxidation. ICAM 2009, setembro 2009, RJ, Brasil. Apresentação na
forma de pôster.
67. V.C. Dias, G. Cerchiaro, P.A. Fiorito, Imobilização de dihidrorodamina 1,2,3 (DHR) em um
eletrodo de ouro modificado com tióis com possível aplicação como sensor de estresse
oxidativo. 32ª Reunião Anual da Sociedade Brasileira de Química, 30 de maio a 02 de junho
de 2009, Fortaleza, CE. Apresentação na forma de pôster.
68. N. S. Guardabaxo, M. P. F. M. Del Lama, D. C. Rodrigues, D. B. Silva, R. M. Z. G. Naal.
Efeito inibidor de substâncias naturais sobre a liberação de mediadores químicos em
mastócitos. 170 Simpósio de Iniciação Científica da Universidade de São Paulo (SIICUSP),
Novembro de 2009, Ribeirão Preto, SP. Apresentação na forma de poster.
69. N. C. Custódio, M. P. F. M. Del Lama, L. A. Deliberto, R. M. Z. G. Naal. Flavonóides/βCyclodextrins inclusio complexes. 7thInternational Congress of Pharmaceutical Sciences
(CIFARP), 6 a 9 de Setembro, 2009,Ribeirão Preto, SP, Apresentação na forma de poster.
70. M. S. Santos, M. P. F. M. Del Lama, M. T. Pupo, R. M. Z. G. Naal. New aryl coumarin
derivatives as inhibitors of RBL-2H3 mast cells degranulation. 7th International Congress of
Pharmaceutical Sciences (CIFARP), 6 a 9 de Setembro, 2009,Ribeirão Preto, SP,
Apresentação na forma de poster.
43
71. N. C. Custódio, L. A. Deliberto, M. P. F. M. Del Lama, R. M. Z. G. Naal. Estudo da
solubilidade de polifenóis pela complexação com β-ciclodextrinas. 170 Simpósio de Iniciação
Científica da Universidade de São Paulo (SIICUSP), Novembro de 2009, Ribeirão Preto, SP.
Apresentação na forma de poster.
72. R. C. S. Luz, F. S. Damos, A. A. Tanaka, L. T. Kubota, Atividade Eletrocatalítica de 2,3,5,6tetracloro-1,4-benzoquinona/Nanotubos de Carbono de Paredes Múltiplas para a Oxidação
de NADH. XVII Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 19 a 23 de Abril de
2009, Fortaleza, CE. Apresentação na forma de poster.
73. R. C. S. Luz, F. S. Damos, A. A. Tanaka, L. T. Kubota, Desenvolvimento de um Filme Fino
Supramolecular Eletroativo Explorando uma Interface Multivalente. XVII Simpósio
Brasileiro de Eletroquímica e Eletroanalítica, 19 a 23 de Abril de 2009, Fortaleza, CE.
Apresentação na forma Oral.
74. R. C. S. Luz, F. S. Damos, A. A. Tanaka, L. T. Kubota, Desenvolvimento e Aplicação de um
Filme Fino Supramolecular como Sensor Amperométrico para Oxigênio. XVII Simpósio
Brasileiro de Eletroquímica e Eletroanalítica, 19 a 23 de Abril de 2009, Fortaleza, CE.
Apresentação na forma de poster.
75. R. C. S. Luz, F. S. Damos, A. A. Tanaka, L. T. Kubota, Y. Gushikem, Eletrocatálise da
Oxidação de L-Glutationa Reduzida por Tetra(N-Metil-4-Piridil) Porfirina de Ferro. XVII
Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 19 a 23 de Abril de 2009, Fortaleza,
CE. Apresentação na forma de poster.
76. M. M. Alves, M. R. Lessa; A.B. Vitoreti, L. M. SILVA, L. T. Kubota, F. S. Damos, R. C. S.
Luz, Detecção Eletroquímica de Hidrazina em Baixo Potencial de Oxidação Empregando
Nanotubos de Carbono e Ftalocianina de Ferro. XVII Simpósio Brasileiro de Eletroquímica
e Eletroanalítica, 19 a 23 de Abril de 2009, Fortaleza, CE. Apresentação na forma de poster.
77. T. C. Cipriano, R. F. Silva, P. M. Takahashi, V. X. Oliveira Jr., W. A. Alves, “Spatial
Organization of Peptide Nanotubes for Electrochemical Devices”. In: 11th International
Conference on Advanced Materials, 20 a 25 de setembro de 2009, Rio de Janeiro, RJ.
Apresentação Oral.
78. A.P. Sousa, A. S. Polo, S. I. Córdoba de Torresi, R. M. Torresi, W. A. Alves, Atividade
Eletrocatalítica da Glicose Oxidase nos Filmes Nanocristalinos de Dióxido de Titânio. XVII
Simpósio Brasileiro de Eletroquímica e Eletroanalítica - XVII SIBEE, 19 a 23 de abril de
2009, Fortaleza, CE. Apresentação na forma de pôster.
79. I.O. Matos, W. A. Alves, Desenvolvimento de Sensores Biomiméticos para Detecção de
Peróxido de Hidrogênio. XVII Simpósio Brasileiro de Eletroquímica e Eletroanalítica - XVII
SIBEE, 19 a 23 de abril de 2009, Fortaleza, CE. Apresentação na forma de pôster.
80. I.O. Matos, P. M. Takahashi, E. L. Bastos, H. Martinho, J. G. Ferreira, C. C. Silva, R. H. A.
Santos, A. Paduan-Filho, A. M. D. C. Ferreira, W. A. Alves, Chloro-Bridged Linear Chain
Imine-Copper(II) Complex and Its Application as Enzymeless Amperometric Biosensor for
44
Hydrogen Peroxide. IV International Symposium on Advanced Materials and Nanostructures,
17 a 19 de maio de 2009, Santo André, SP. Apresentação na forma de pôster.
81. A.de Lima, W. A. Alves, T. M. Benedetti, S. I. Córdoba de Torrei, R. M. Torresi,
Mediatorless Biosensor for H2O2 Based on Recombinant Forms of Microperoxidase-11
Directly Adsorbed on Polycrystalline Gold. IV International Symposium on Advanced
Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP. Apresentação na
forma de pôster.
82. I.O. Matos, W. A. Alves, Approaches for Multicopper Oxidases in the Desing of Novel
Electrochemical Sensors for Analytical Applications. IV International Symposium on
Advanced Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP.
Apresentação na forma de pôster.
83. W. Alves, T. C. Cipriano, P. M. Takahashi, V. X. de Oliveira Junior, W. A. Alves,
Modification on Peptide Nanotubes by Gold Nanoparticles. IV International Symposium on
Advanced Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP.
Apresentação na forma de pôster.
84. T. C. Cipriano, W. Alves, P. M. Takahashi, V. X. de Oliveira Junior, W. A. Alves, Interaction
of Peptide Nanotubes with Polyelectrolyte Self-Assembled Films. IV International Symposium
on Advanced Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP.
Apresentação na forma de pôster.
85. W. A. Alves, A. C. Sant´Ana, M. P. Abbott, P. H. Mello, J. G. Ferreira, R. H. A. Santos, M.
L. A. Temperini, A. Paduan-Filho, A. M. D. C. Ferreira, Structural and Spectroscopic
Characterization of a New Dinuclear Schiff Base Azido-Bridged Copper(II) Complex. IV
International Symposium on Advanced Materials and Nanostructures, 17 a 19 de maio de
2009, Santo André, SP. Apresentação na forma de pôster.
86. C. P. Sousa, A. S. Polo, W. A. Alves, Nanocrystalline TiO2 for Electrochromic Electrodes
Using 1,1-Bis(4-Carboxybenzil)-4,4-Bipyridinium Di-Bromide. IV International Symposium
on Advanced Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP.
Apresentação na forma de pôster.
87. M. T. Ximenes, W. A. Alves, V. X. de Oliveira Junior, P. A. Fiorito, K. M. Honorio, P. H.
Mello, Theoretical Study on Redox Properties of a Laccase and an Ascorbato Oxidase. IV
International Symposium on Advanced Materials and Nanostructures, 17 a 19 de maio de
2009, Santo André, SP. Apresentação na forma de pôster.
88. P. M. Takahashi, T. C. Cipriano, W. Alves, V. X. de Oliveira Junior, W. A. Alves, The
Influence of pH on the Formation of Peptides Nanostructures. IV International Symposium
on Advanced Materials and Nanostructures, 17 a 19 de maio de 2009, Santo André, SP.
Apresentação na forma de pôster.
89. W. A. Alves, I. O. Matos, T. L. Ferreira, M. Bertotti, Estudo Eletrocatalítico de um Sistema
Biomimético da Enzima Multinuclear de Cobre Frente à Redução de Oxigênio. 32ª. Reunião
45
Anual da Sociedade Brasileira – 32ªRASBQ, 30 de maio a 02 de junho de 2009, Fortaleza,
CE. Apresentação na forma de pôster.
90. I.O. Matos, W. A. Alves, Sensor Biomimético para Determinação e Monitoramento de
Peróxido de Hidorgênio em Solução. 32ª. Reunião Anual da Sociedade Brasileira –
32ªRASBQ, 30 de maio a 02 de junho de 2009, Fortaleza, CE. Apresentação na forma Oral.
91. P. M. Takahashi; T. C. Cipriano, W. Alves, V. X. de Oliveira Junior, W. A. Alves, Estudo da
Influência do pH na Formação de Nanoestruturas Peptídicas e da sua Interação com
Nanopartículas de Ouro. 32ª. Reunião Anual da Sociedade Brasileira – 32ªRASBQ, 30 de
maio a 02 de junho de 2009, Fortaleza, CE. Apresentação na forma de pôster.
92. T. C. Penna; M. P. Massafera, I. Riou, O. Chauvet,; S. I. C. Torresi, R. M. Torresi. Síntese e
Caracterização de Nanocompósitos de Nanotubos de Carbono e Poli(anilina) Preparados em
Líquidos Iônicos. XVII Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 2009,
Forataleza, Brasil. Apresentação na forma de pôster.
93. M. P. Massafera, C. D-Chouvy; S. I. C. Torresi. Poly(pyrrole) nanopores and nanowires as
ammonia sensing platforms 60th Annual Meeting of the International Society of
Electrochemistry, 2009, Beijing. China.
94. M. P. Massafera; C. D-Chouvy; S. I. C. Torresi. Synthesis and Characterization of
Poly(pyrrole) Nanopores and Nanowires and Application as Ammonia Sensors. 11th
International Conference on Advanced Materials, 2009, Rio de Janeiro. Brasil. Apresentação
na forma Oral.
95. L. Kanashiro; M. P. Massafera, O. Chauvet, S. I. C. Torresi, R. M. Torresi. Comportamento
eletroquímico de compósitos de poli(anilina) e nanotubos de carbono preparados em líquido
iônico. 33ª Reunião Anual da Sociedade Brasileira de Química, 2010, Águas de Lindóia.
Brasil.
96. M. P. Massafera, S. I. C Torresi. Otimização da síntese de nanofios de poli(pirrol) e sua
influência na detecção de amônia. SIBAE, 2010, Madrid. Espanha.
97. T. M. Benedetti, V. R. Gonçales, S.IC. Torresi, R. M. Torresi. Eletrodeposição de MnO2
sobre molde de partículas coloidais e caracterização eletroquímica em diferentes eletrólitos.
In: XVII Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 2009, Fortaleza. Brasil.
Apresentação na forma oral.
98. A.S. Navarro, V. R. Gonçales, S. I. C. Torresi. Síntese e caracterização de nanopartículas de
hexacianoferrato de cobre para detecção de H2O2. Potencial aplicação em biossensores.
XVII Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 2009, Fortaleza. Brasil.
99. V. R. Gonçales, M. H. Gaitán, L. Baraldo, S. I. C. Torresi. Síntese template de plataformas
nanoestruturadas para biossensores de colina. XVII Simpósio Brasileiro de Eletroquímica e
Eletroanalítica, 2009, Fortaleza. Brasil. Apresentação na forma Oral.
46
100.
M. H. Gaitán, V. R. Gonçales, G. J. A. A. Soler-Illia, S. I. C. Torresi, L. M. Balardo.
Efectos del tamaño/estructura en Azules de Prusia autoensamblados sobre materiales
mesoporos de TiO2. IX Encuentro CNEA, 2009, Bariloche. Argentina.
101.
M. H. Gaitán, V. R. Gonçales, G. J. A. A. Soler-Illia, L. M. Balardo, S. I. C. Torresi.
Structure/size effects of self-assembled Prussian blue confined in highly organized
mesoporous TiO2 on the electrocatalytic properties towards H2O2 detection. 60th Annual
Meeting of the International Society of Electrochemistry, 2009, Beijing. China. Apresentação
na forma Oral.
102.
T. M. Benedetti, V. R. Gonçales, S. I. C. Torresi, R. M. Torresi. Wettability and
Electrochemical studies of macroporous MnO2 films in hydrophobic and hydrophilic ionic
liquids. 11th International Conference on Advanced Materials and VIII Encontro SBPMat,
2009, Rio de Janeiro. Brasil. Apresentação na formaOral.
103.
P. Ponce, V. R. Gonçales, A. Lugão, S. I. C. Torresi. The Performance of Starch Films
Electrolyte for Bismuth Electrodeposition Devices. 216th Electrochemical Society Meeting,
2009, Vienna. Apresentação na forma Oral.
104.
V. R. Gonçales, M. H. Gaitán, G. J. A. A. Soler-Illia, L. M. Baraldo, S. I. C. Torresi.
Choline Biosensors based on Mesoporous and Macroporous Prussian Blue Analogues.
Size/confinement Effects. 216th Electrochemical Society Meeting, 2009, Viena. Apresentação
na forma Oral.
105.
T. M. Benedetti, V. R. Gonçales, D. Petri, S. I. C. Torresi, R. M. Torresi. Wettability
Effects on Macroporous MnO2 Electrodes by Hydrophobic or Hydrophilic Ionic Liquids.
216th Electrochemical Society Meeting, 2009, Viena. Apresentação na forma Oral.
106.
D. G. B. Limachi, L. T. Silveira, E. P. Cintra, S. I. C. Torresi. Eletroatividade do
polímero condutor poli(5-amino-1-naftol) em líquido iônico. III semana de Tecnologia do
Instituto Federal de Educação, Ciência e Tecnologia, 2009, São Paulo. Brasil.
107.
A.F. Camilo; L. T. Silveira, O. N. Oliveira Jr, L. Caseli, S. I. C. Torresi. Filmes Langmuir
Blodgett de um copolímero solúvel da polianilina. 33ª Reunião Anual da Sociedade Brasileira
de Química, 2010, Águas de Lindóia. Brasil.
108.
L. T. Silveira; D. Limache, E. P. Cintra, R. M. Torresi, S. I. C. Torresi.
Eletropolimerização e caracterização eletroquímica do polímero condutor poli(5-amino-1naftol) em líquido iônico. 33ª Reunião Anual da Sociedade Brasileira de Química, 2010,
Águas de Lindóia. Brasil.
109.
L. A. Deliberto; Z. Naal, Bipyridinium pyrrol derivative electropolymerized film as a
mediator for flunitrazepam reduction. 7th International Congress of Pharmaceutical Sciences
CIFARP, 6 a 9 de Setembro de 2009, Ribeirão Preto, SP.
47
110.
A.M. M. Azevedo, Z. Naal, Polybenzylvilogen films as a mediator for dioxygen
reduction. 7th International Congress of Pharmaceutical Sciences CIFARP, 6 a 9 de Setembro
de 2009, Ribeirão Preto, SP.
111.
T.C. Canevari, L.T. Arenas, Y. Gushikem, E.V. Benvenutti, “SiO2 mixed oxide prepared
by the sol-gel method using HCl and HF as catalysts: Study of SnO2 particles dispersion in
the matrices”, XV International Sol-Gel Conference(so9l-gel `09), August 23-27, Porto de
Galinhas, Pe, p.123 (2009).
112.
C.M. Trindade, C.C. Moro, Y. Gushikem, T.M.H. Costa, E.V. Benvenutti, “Insertion of
silica xerogel in interlayer spce of bidimensional prtonated aminopropil silsesquioxane”, XV
International Sol-Gel Conference(so9l-gel `09), August 23-27, Porto de Galinhas, Pe, p.234
(2009).
113.
A.V. Panteleimonov, S.A. Miyerniy, H.A. Magosso, Y. Gushikem, Y.V. Kholin,
“Cooperativity effects at adsorption on surface of silica-organic hybrid materials”, Modern
Problems of Physical Chemistry 2009, Donetsk 2009, Ukraine, p. 202-203, (2009)
114.
Magosso, H. A. ; Ramos, A. R.; Gushikem, Y . 3-N-Propyl-1-Azonia-4Azabicyuclo[2.2.2]Octane
Silesequioxane
Chloride:
Simultaneous
Voltammetric
Determination Of Ascorbic Acid, Dopamine And Uric Acid. In: 11th International Conference
On Advanced Materials; VIII Econtro Da Sbpmat, 2009, Rio De Janeiro. ICAM 2009. Rio De
Janeiro, 2009.
115.
Arenas, L. T. ; Canevari, T. ; Gushikem, Y. . SiO2/Nb2O5/Graphite carbon ceramic
conducting material: preparation, characterization and its use as electrochemical sensor for
4-aminophenol. In: 11th International conference on advanced materials; VIII Econtro da
SBPMat, 2009, Rio de Janeiro. ICAM2009. Rio de Janeiro, 2009.
116.
Abdur R.; Arenas, L. T. ; Gushikem, Y. . Synthesis of SiO2/C-graphite mesoporous
mateiral and their electrochemical study. In: 11th International conference on advanced
materials; VIII Econtro da SBPMat, 2009, Rio de Janeiro. ICAM2009. Rio de janeiro, 2009.
117.
Barros, S.B.A. ; Arenas, L. T. ; Abdur Rahim ; Gushikem, Y. . Effect of HF in the
preparation of carbon-ceramic materials by sol-gel process. In: 11th International conference
on advanced materials; VIII Encontro SBPMat, 2009, Rio de Janeiro. ICAM2009. Rio de
Janeiro, 2009.
118.
Lage, R. R.; Miyata, M E V; Sigoli, F. A.; Kubota, L.T.; Yoshida, I.V.P.; Study of
electrical conductive C/ SiCxOy ceramic composites, 09/2009, Científico Internacional, XII
International Conference on the Physics of Non-Crystalline Solids (PNCS XII) and 9th
International Symposium on Crystallization in Glasses and Liquids (Crystallization 2009),
Vol. 0415, pp.161-161, Foz do Iguaçu, PR, BRASIL, 2009.
119.
Nesterenko, P.N.; Dias, J.C.; Dicinoski, G.W.; Kubota, L.T.; Haddad, P.R.;
Determinations of trace metals in ethanol fuel by high performance chelation ion
48
chromatography with post column reaction and photometric detection, 21st International Ion
Chromatography Symposium – IICS 2009, pag. 114, Dublin, Ireland, 2009.
120.
L.A.F. Godoy Jr, M.P. Pedroso, E.C. Ferreira, L.W. Hantao, R.J. Poppi, F. Augusto,
Quantitative Analysis by Comprehensive Bidimensional Gas Chromatography using Interval
Multi-way Partial Least Squares Calibration, 11th International Symposium on Hyphenated
Techniques in Chromatography and Hyphenated Chromatographic Analyzers (HTC-11), 25 a
29 de Janeiro de 2010, Brugge, Bélgica. Apresentação na forma oral.
121.
S.R. Rivellino, L.W. Hantao, S. Risticevic, R.C.R. Camargo, E. Carasek, J. Pawliszyn, F.
Augusto, Extraction Artifacts and GC×GC Profiling of Honey, 11th International Symposium
on Hyphenated Techniques in Chromatography and Hyphenated Chromatographic Analyzers
(HTC-11), 25 a 29 de Janeiro de 2010, Brugge, Bélgica. Apresentação na forma de poster.
122.
M.P. Pedroso, L.W. Hantao, E.C. Ferreira, F. Augusto, Identification of Volatile
Compounds from Pineapple (Ananas Comosus) Pulp by GC×GC-FID and GC-MS, 11th
International Symposium on Hyphenated Techniques in Chromatography and Hyphenated
Chromatographic Analyzers (HTC-11), 25 a 29 de Janeiro de 2010, Brugge, Bélgica.
Apresentação na forma de poster.
123.
M.P. Pedroso, L.W. Hantao, E.C. Ferreira, F. Augusto, Estruturação Cromatográfica:
uma Ferramenta para a Análise de Óleos Essenciais e Correlatos por GC×GC-FID, 5o
Simpósio Brasileiro de Óleos Essenciais (SBOE), 3 a 6 de Novembro de 2009, Rio de Janeiro,
RJ. Apresentação na forma de poster.
124.
L.A.F. Godoy Jr, M.P. Pedroso, E.C. Ferreira, F. Augusto, R.J. Poppi, Interval Multi-Way
Partial Least Squares (iNPLS) for Quantification of Allergenics in Perfume using
Comprehensive Two-Dimensional Gas Chromatography (GC×GC), 15th Euroanalysis, 6 a 10
de Setembro de 2009, Innsbruck, Áustria. Apresentação na forma de poster.
125.
M.P. Pedroso, F. Augusto, E.C. Ferreira, Identificação de compostos voláteis de abacaxi
(Ananas comosus) por GCxGC-FID e GC/MS, XV Encontro Nacional de Química Analítica e
III Congresso Iberoamericano de Química Analítica, 18 a 21 de Outubro de 2009, Salvador,
BA. Apresentação na forma de poster.
126.
M. S. Aurora-Prado, G. A. Micke, M. F. M. Tavares, K. D. Altria, Microemulsion
electrokinetic chromatography for the separation and identification of water- and fat-soluble
vitamins. 15th Latin American Symposium on Biotechnology, Biomedical, Biopharmaceutical,
and Industrial Applications of Capillary Electrophoresis and Microchip Technology - LACE,
2 a 6 de Outubro de 2009, Sevilha, Espanha. Apresentação na forma de poster.
127.
A. A. B. Canuto, F. G. Tonin, V. M. Carpentieri, M. F. M. Tavares, J. P.S. Farah, QSRR
evaluation of the interaction between flavonoids and SDS micelles. 15th Latin American
Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of
Capillary Electrophoresis and Microchip Technology - LACE, 2 a 6 de Outubro de 2009,
Sevilha, Espanha. Apresentação na forma de poster.
49
128.
C. O. Costa, G. A. Micke, M. F. M. Tavares, Evaluation of stacking with reverse
migrating micelles and matrix removal procedure for the MEKC analysis of anionic
compounds in environmental water. 15th Latin American Symposium on Biotechnology,
Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and
Microchip Technology - LACE, 2 a 6 de Outubro de 2009, Sevilha, Espanha. Apresentação
na forma de poster.
129.
A.Silva, M. F. M. Tavares, Determination of inorganic contaminants in alcohol fuel by
capillary electrophoresis. 15th Latin American Symposium on Biotechnology, Biomedical,
Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip
Technology - LACE, 2 a 6 de Outubro de 2009, Sevilha, Espanha. Apresentação na forma de
poster.
130.
V. do Nascimento, F. G. Tonin, M. F. M. Tavares, Validation of a capillary
electrophoretic method for the quantitative determination of alkaloids in cinchona bark. 15th
Latin American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and
Industrial Applications of Capillary Electrophoresis and Microchip Technology - LACE, 2 a 6
de Outubro de 2009, Sevilha, Espanha. Apresentação na forma de poster.
131.
T. S. Fukuji, F. G. Tonin, M. F. M. Tavares, Evaluation of extraction procedures for the
CE determination of phenolic acids in Euterpe oleraceae food products. 15th Latin American
Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of
Capillary Electrophoresis and Microchip Technology - LACE, 2 a 6 de Outubro de 2009,
Sevilha, Espanha. Apresentação na forma de poster.
132.
T. S. Fukuji, C. A. Silva, M. F. M. Tavares, Determination of folic acids in wheat flour by
capillary electrophoresis. 15th Latin American Symposium on Biotechnology, Biomedical,
Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip
Technology - LACE, 2 a 6 de Outubro de 2009, Sevilha, Espanha. Apresentação na forma de
poster.
133.
Klassen, M. F. M. Tavares, Development of a CZE method for the analysis of banned
aromatic amines originated from azo dyes cleavage. 15th Latin American Symposium on
Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary
Electrophoresis and Microchip Technology - LACE, 2 a 6 de Outubro de 2009, Sevilha,
Espanha. Apresentação na forma de poster.
134.
E. P. Moraes, M. S. Tavares, J. P. S. Farah, M. F. M. Tavares, CE-MS investigation of
biomarkers in urine towards a non-invasive diagnosis of vesicoureteral reflux. 15th Latin
American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial
Applications of Capillary Electrophoresis and Microchip Technology - LACE, 2 a 6 de
Outubro de 2009, Sevilha, Espanha. Apresentação na forma de poster.
135.
E. P. Moraes, F. G. Tonin, C. A. Fuzo, L. G. Dias, J. P. S. Farah, M. F. M. Tavares,
Assessment of solute-micelle interactions in MEKC using quantitative structure-retention
relationships. 15th Latin American Symposium on Biotechnology, Biomedical,
50
Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip
Technology - LACE, 2 a 6 de Outubro de 2009, Sevilha, Espanha. Apresentação na forma
oral.
136.
Z. Buzatto, J. A. Farfan, A. V. C. Simionato, Development of a method for the assessment
of tumor biomarkers by micellar electrokinetic capillary chromatography. XV Congresso
Latino Americano de Eletroforese Capilar - LACE, 2 a 6 de outubro de 2009, Sevilla,
Espanha. Apresentação na forma de painel.
137.
Z. Buzatto, A. A. Leitão, J. C. Pierozzi, J. A. Farfan, A. V. C. Simionato, Otimização das
variáveis de um método de cromatografia micelar eletrocinética capilar para a análise de
nucleosídeos. XXXII Reunião Anual da Sociedade Brasileira de Química - SBQ, 30 de maio a
2 de junho de 2009, Fortaleza, CE. Apresentação na forma de painel.
138.
R. H. Medeiros, A. V. C. Simionato, Otimização de um método de separação de
nucleosídeos modificados e não modificados, biomarcadores tumorais, por HPLC-DAD. I
Congresso Analítica, 8 a 10 de setembro de 2009, São Paulo, SP. Apresentação na forma oral
e painel.
139.
M. Ribani, T. do Valle, E. L. da Silva, C. H. Collins, C. B. G. Bottoli, Obtenção de ácido
valerênico a partir de extrato seco de valeriana por cromatografia líquida preparativa. 32°
Reunião Anual da Sociedade Brasileira de Química, 30 de maio a 02 de junho de 2009,
Fortaleza, CE. Apresentação na forma de pôster.
140.
M. J. S. Gutiérrez-Ponce, C. R. Silva, C. B. G. Bottoli, Separation of some drugs by
capillary electrochromatography using a new monolithic silica phase. 15th Latin American
Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of
CAPILLARY ELECTROPHORESIS and Microchip Technology – LACE 2009, 3 a 6 de
outubro de 2009, Sevilha, Espanha. Apresentação na forma de poster.
141.
J. A. F. da Silva, W. K. T. Coltro, E. Carrilho, Contactless conductivity detection on
microchip platforms: From small ions to biomolecular interactions. Lab Automation, 25 a 28
de janeiro de 2009, Palm Springs, CA, USA. Apresentação na forma oral.
142.
J. A. F. da Silva, P. T. V. Rosa, T. R. Tonon, Avaliação do comportamento eletroquímico
de lapachol e beta-lapachona na presença de tensoativos aniônicos e catiônicos. XVII
Simpósio Brasileiro de Eletroquímica e Eletroanalítica, 19 a 23 de abril de 2009, Fortaleza,
CE. Apresentação na forma de pôster.
143.
J. A. F. da Silva; N. V. Castro, D. P. Jesus, A. F. Faria, M. V. N. Souza, M. A. L.
Oliveira, Determinação rápida de etambutol em formulações farmacêuticas por eletroforese
capilar com detecção condutométrica sem contato. 15º Encontro Nacional de Química
Analítica, 2009, Salvador, BA. Apresentação na forma de pôster.
144.
A.R. S. Araújo, J. A. F. da Silva, R. E. Bruns, Avaliação do formato do sinal de excitação
empregado em detecção condutométrica sem contato acoplada a eletroforese capilar. XVII
51
Congresso Interno de Iniciação Científica da Unicamp, 23 e 24 de setembro de 2009,
Campinas, SP.
145.
N. V. Castro, J. A. F. da Silva, Desenvolvimento de métodos para a determinação de
etambutol por eletroforese capilar de zona com detecção condutométrica sem contato. XVII
Congresso Interno de Iniciação Científica da Unicamp, 23 e 24 de setembro de 2009,
Campinas, SP.
146.
G. R. M. Duarte, C. W. Price, B. Poe, E. Carrilho , J. P. Landers, Valveless Integration of
IR-PCR Amplification and Electrophoresis in Polyester-Toner Microchips. 35th International
Symposium on High Performance Liquid Phase Separations and Related Techniques, 2010,
Boston, MA, USA. Apresentação forma pôster.
147.
R. S. Lima, U. P. R. Filho, P. A. P. Nascente, M. H. O. Piazzetta, A. L. Gobbi, V. L.
Pimentel, W. K. T. Coltro, E. Carrilho, Caracterização de Processos de Silanização em
Superfície de SiO2 por MEV, XPS e AFM para Construção de Biossensores. 20ª Reunião
Anual de Usuários do Laboratório Nacional de Luz Sincroton, Campinas-SP, 2010.
Apresentação forma de pôster.
148.
G. R. M. Duarte, J. C. Borba ; W. K. T. Coltro, E. Carrilho, DNA Separation in
Polyester-toner Electrophoresis Microchips. 23rd International Symposium on Microscale
Bioseparations, MSB 2009, Boston, MA, USA. Apresentação forma de pôster.
149.
S. B. GUTERRES, E. Carrilho, W. F. GATTAZ, Analysis of isoforms of calcium
independent phospholipase A2 in human platelets. 11th International Congress on Amino
Acids, Peptides and Proteins. August 3 – 7, 2009, Vienna, Austria. Apresentação forma de
pôster.
150.
R. M. Duarte, C. W. Price, B. Poe, E. Carrilho, J. P. Landers, Dynamic Solid Phase
Extraction and PCR Amplification in Polyester-Toner Microchip. Latin American
Symposium on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of
Capillary Electrophoresis and Microchip Technology (LACE), 2009, Sevilla- Espanha.
Apresentação forma pôster.
151.
R. M. Duarte, C. W. Price, W. T. Coltro, J. C. Borba, J. P. Landers, E. Carrilho, Análise
genéticas em microchip de poliéster-toner.15º Encontro Nacional de Química Analítica ENQA, 18 a 21 de Outubro de 2009, Salvador, BA. Apresentação forma pôster.
152.
G. R. M. Duarte, C. W. Price, J. P. Landers, E. Carrilho, Extração dinâmica de DNA em
fase sólida com partículas magneticamente controladas em microchip de vidro para
diagnósticos clínicos.15º Encontro Nacional de Química Analítica - ENQA, 18 a 21 de
Outubro de 2009, Salvador, BA. Apresentação forma pôster
153.
Fraige, E. Carrilho, Índice de acidez total e ácidos orgânicos em uvas viníferas como
indicador de maturação. 15º Encontro Nacional de Química Analítica - ENQA, 18 a 21 de
Outubro de 2009, Salvador, BA. Apresentação forma pôster.
52
4) SUPERVISIONS RELATED TO INCTBio
4.1) Concluded Supervision
Renata Anselmo Oseas da Silva (Iniciação Científica). IQ-Unicamp Bolsista CNPq.
Adilson Roberto Brandão (Mestrado). IQ-Unicamp Bolsista FAPESP.
Alessandra Sussulini (Doutorado). IQ-Unicamp Bolsista FAPESP.
Daniela Dal Molin (Mestrado). UFSM - Bolsista CAPES.
Diogo Pompéu de Moraes (Doutorado). UFSM Bolsista CAPES.
Suelem Harumi Takahashi (Mestrado). IQ-USP Bolsista CNPq.
Iorquirene de Oliveira Matos (Mestrado). UFABC Bolsista UFABC.
Francisco de Assis dos Santos Silva.(mestrado). UFAL Bolsista CNPq.
José Rui Machado Reys. UFAL (Doutorado)
Phabyanno Rodrigues Lima (Doutorado). IQ-Unicamp Bolsista FAPESP.
José de Ribamar Martins Neto (Mestrado). UFMA Bolsista CAPES.
Wilney de Jesus Rodrigues Santos (Doutorado). IQ-UnicampBolsista FAPESP.
Murilo Santhiago (Mestrado). IQ-Unicamp Bolsista CNPq.
Jailson Cardoso Dias (Doutorado). IQ-Unicamp Bolsista FAPESP.
Márcio Pozzobon Pedroso (Doutorado). Bolsista CNPq.
Juliana Vieira Alberice (Mestrado) Bolsista CNPq
5.2) Current supervision
Lidiane Raquel Verola Mataveli (Doutorado). Bolsista FAPESP.
Herbert de Sousa Barbosa (Doutorado). Bolsista CAPES.
Marcelo Anselmo Oseas da Silva (Doutorado). Bolsista FAPESP.
Heloisa França Maltez (Pós-doutorado). Bolsista FAPESP.
Fábio Arlindo Silva (Doutorado) – Bolsista FAPESP.
Renato de Cássio Ferreira Neves (Doutorado) – Bolsista CAPES.
Felipe André dos Santos (Mestrado) – Bolsista FAPESP.
Paula Monteiro de Lima (Mestrado) – Bolsista FAPESP.
Mayra Anton Dib Saleh (Mestrado) – Bolsista FAPESP.
Lucas Rossi Sartori (Mestrado). Bolsista FAPEMIG.
Fernanda Fantin Fernandes (Mestrado). Sem bolsa.
Mariana Gava Segatelli (Pós-Doutorado). Bolsista CAPES-PNPD.
Vivian Silva Santos (Iniciação Científica). Bolsista FAPEMIG.
Letícia Ramos Nacano (Iniciação Científica). Bolsista FAPEMIG.
Thiago Carvalho de Ávila (Iniciação Científica). Bolsista CNPq.
Rodrigo Cunha Wanick (Doutorado). Bolsista CNPq.
Leonardo Revoredo Frazão (Iniciação Científica). CNPq/PIBIC.
Mariana Fioramonte (Mestrado). Bolsista CNPq.
Alexandre Ferreira Gomes (Mestrado). Bolsista FAPESP.
Alana dos Reis Figueuredo. (Doutorado). Bolsista FAPESP.
Eduardo Jorge Pilau. (Doutorado). Bolsista CNPq.
Luiz Fernando Arruda Santos. (Doutorado). Bolsista CNPq.
Hugo Juarez Vieira Pereira (Pós-Doutorado). Bolsista FAPESP.
Camila De Lellis Knorr (Mestrado). Sem bolsa.
Lucas Schmidt. (Mestrado). INCT-CAPES.
53
Juliana Felizolla (Pós-doc). CAPES-PNPD.
Thiago Araujo (Doutorado). Bolsista Inmetro.
Rachel Ann Hauser Davis (Doutorado). Bolsista CNPq.
Alex Lima (Iniciação Científica). Bolsista PIBIC-CNPq.
Márcia Cristina Breitkreitz (Doutorado). Bolsista FAPESP.
Monica Benicia Mamian Lopez (Doutorado). Bolsista INCT-CAPES.
Cristina Donizeti Bernardes (Mestrado). Bolsista CAPES.
Marcela de Souza Santos (Doutorado). Bolsista Fapesp.
Vinicius Romero Gonçales (Doutorado) Bolsista Fapesp
Mariana Pereira Massafera (Doutorado) Bolsista Fapesp
Suelem Harumi Takahashi (Doutorado) Bolsista CNPq
Tatiana Augusto (Doutorado) Bolsista CNPq
Leonardo Teixeira Silveira (Doutorado) Bolsista Capes.
Iorquirene de Oliveira Matos (Doutorado). Bolsista Fapesp.
Wellington Alves (Mestrado) Bolsita Fapesp
Thiago de Carvalho Cipriano (Mestrado) Bolsista Fapesp
Heliane Rosa do Amaral (Mestrado) Bolsista CNPq
Michelle da Silva Liberato (Mestrado) Bolsista Fapesp
Kelly Galhardo (Mestrado) Bolsista CNPq.
Vinicius Cesar Dias (Mestrado). Bolsista UFABC
Dênio Emanuel Pires Souto (Mestrado). Bolsista INCT-CAPES.
Daniele Franciscato Paggi (Iniciação Científica). Bolsista FAPESP.
Allan de Oliveira Bragatto (Iniciaçao Cientifica) Bolsista CNPq
Renata Zambelli Rodrigues (Iniciação Científica). Bolsista Fapesp.
Naiara de Cassia Custódio (Iniciação Científica). Bolsista Fapesp.
Natali Salotti Guardabaxo (Iniciação Científica). Bolsista Pibic-CNPq.
Carla Primo (Iniciação Científica). Sem bolsa.
Ana Maria Milanez Azevedo (Iniciação Científica) Bolsista CNPq.
Laila A. Deliberto (Aperfeiçoamento) sem Bolsa.
Ana Caroline Ferreira Santos (Iniciação Científica). Bolsista CNPq.
Gláucia Tinoco Corrêa (Iniciação Científica). Bolsista CNPq.
José Rodrigues Delfino (Iniciação Científica). Bolsista CNPq.
Ana Paula Mota Ferreira (Mestrado). Bolsista CAPES
Natalia Fattori, (Mestrado). Bolsista Fapesp
Herica Aparecida Magosso, (Pos-doutorado). Bolsista Fapesp.
Mirian Paula dos Santos, (Pos-doutorado). Bolsista Fapesp.
Leliz Ticona Arenas, (Pos-doutorado). Bolsista Fapesp.
Camila Marchetti Maroneze, (Pos-doutorado). Bolsista CNPq.
Thiago da Cruz Canevari, (Doutorado). Bolsista CAPES.
Abdur Rahim, (Doutorado). Bolsista CNPq/TWAS.
Lucas Samuel Soares dos Santos, (Mestrado). Bolsista CNPq.
Rafaela Fernanda Carvalhal (Doutorado). Bolsista CNPq.
Luciana F. Martins (Doutorado). Bolsista CNPq.
José Tiago Barragan (Doutorado). Bolsista Metrohm.
Murilo Santhiago (Doutorado). Bolsista CAPES.
Danielle C.M. Ferreira (Pós-doutorado). Bolsista FAPESP.
Renata Kelly Mendes (Pós-doutorado). Bolsista FAPESP.
54
Leandro Wang Hantao (Mestrado). Bolsista CAPES.
Luiz Antonio Fonseca de Godoy Junior (Doutorado). Bolsista CNPq / co-orientador.
Sandra Regina Rivellino Marques (Doutorado). Bolsista CNPq.
Ernesto Corrêa Ferreira (Doutorado). Sem bolsa.
Aline de Paula Vitor (Mestrado). Bolsista CNPq.
Edgar Perin Moraes (Pós-doutorado). Bolsista FAPESP.
Adriana Zardini Buzatto (iniciação científica). Bolsista FAPESP.
Rafael Henrique Medeiros (iniciação científica). Bolsista FAPESP.
Marcelo Fabiano André (Doutorado). Bolsista CAPES.
Maria de Jesús Santa Ponce Gutiérrez (Doutorado). Bolsista CNPq.
Hugo Richard Silva Araújo (Mestrado). Bolsista CNPq.
Bruna Regina Toledo Sampaio (Iniciação Científica). Bolsista CNPq.
Valeska Soares Aguiar (Mestrado). Bolsista Fapesp.
Dr. Alexandre Zatkovskis Carvalho (Pós-Doutorado). Bolsista FAPESP.
Richard Piffer Soares de Campos (Mestrado). Bolsista CAPES.
Thais Rodrigues Tonon (Iniciação Científica). Bolsista CAPES.
Letícia Souza Leite Vieira (Iniciação Científica). Bolsista CNPq.
Debora Pastorin da Silva (Iniciação Científica). Solicitada, FAPESP.
Maribel Elizabeth Funes Huacca (Pos-Doc). Bolsista CNPq/INCTBio.
Sheila Barreto Guterres (Doutorado). Bolsista Fapesp.
Karina Fraige (Doutorado). Bolsista Fapesp.
Gabriela Duarte (Doutorado). Bolsista FAPESP.
Patrícia Eugenio (Doutorado). Bolsista CNPq.
Juliane Cristina Borba (Mestrado) sem bolsa.
Thiago Pinotti Segato (Doutorado) Bolsista Capes.
Renato Sousa Lima (Mestrado) Bolsista CNPq.
Jenifer Ferrezini (Treinamento Técnico). Bolsista FAPESP.
5) Prizes, patents required/obtained and/or other relevant information.
Patent:
1. Santana MHA ; Correia CRD ; Gozzo, FC ; Martinez PDG ; Macedo FM ; Saito EY .
Processo de Produção de Conjugados de Interferon com Óxidos de Polialquilenos e Seus
Usos. 2009, PI0804889-4.
2. Santana MHA ; Correia CRD ; Gozzo, F. C. ; Martinez PDG ; Macedo FM ; Saito EY .
Processo de Produção de Carbonatos Ativados de Óxidos de Polialquileno e Seus Usos.
2009, PI0803944-5.
3. M. O. F. Goulart, J. R. M. Reys, P. R. Lima, F. C. De A. Galdino, L. T. Kubota. Dispositivo
sensors, processo de preparação do dispoitivo, método para detecção de artesinina em
amostras multicomponentes, e uso. PI. 018090004683.
4. Kubota, L.T.; Kisner, A.; Diniz, J.A.; Cavarsan, F.A.; Processos de obtenção de dispositivos
eletrônicos a base de nanoeletrodos e dispositivos eletrônicos obtidos a partir dos mesmos,
PI. 018090014945.
55
5. 2010: Patente depositada nos EUA em 02 de Março de 2010, G. R. M. DUARTE, E.
CARRILHO, J. P. Landers . “Method and System for Dynamic Solid Phase Extraction and
PCR Amplification in Polyester-Toner (PeT) microchip: fabrication of multilayer devices for
large depth microfluidic architecture”
6. 2009: Patente requerida INPI: W. K. T. Coltro, E. Carrilho, J. A. F. da Silva, Aparelho
detector condutométrico sem contato para biossensores microfluídicos.
Prizes:
1. Trabalho premiado em primeiro lugar no 2010 Winter Conference on Plasma
Spectrochemistry (04-09/01/2010, Fort Myers, FL, USA): Iodine determination in food by
ICP-MS after digestion by MIC (Friday Session), ICP Information Newsletter.
2. Award ICP Information Newsletter, apresentado no XXXVI CSI, realizado em Budapeste de
30/08 a 03/09/2009 (Sample preparation based on MIC for determination of halogens in
elastomers, total de 211 trabalhos), Hungarian Chemical Society, Hungria.
3. Melhor trabalho no 15o ENQA – Sessão de Materiais Biológicos (Salvador, BA) em 1821/10/2009 - Influência da pressão de O2 sobre a eficiência de decomposição de fígado
bovino em forno de microondas com ácido diluído (TR114), Sociedade Brasileira de Química
e Universidade Federal da Bahia.
4. Trabalho premiado em 1o lugar no Congresso Analítica Latin America, realizado de 08 a
10/09/2009 no Transamerica Expo Center, SP: Decomposição de amostras refratárias com
MIC por IC, ICP-OES e ICP-MS, Nurnberg Messe - Analitica Latin America.
5. Trabalho premiado como um dos três melhores posters apresentados no 1st Ibero-American
Meeting on Toxicology and Environmental Health: Taylor & Francis Book Award, Journals
of Toxicology and Environmental Health and Taylor & Francis Group. 2009.
6. S.I.C. de Torresi, Prêmio Novas Fronteiras. Pro-Reitoria de Pós-Graduação, Universidade de
São Paulo, 2009.
7. Menção Honrosa: Poster apresentado no 7th CIFARP-2009 - L.A Deliberto, Z. Naal,.
Bipyridinium pyrrol derivative electropolymerized film as a mediator for flunitrazepam
reduction., Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP.
8. Y. Gushikem, Membro titular da Academia Brasileira de Ciências (2009).
9. Y. Gushikem, Medalha E.J.S. Vichi concedida pela SBQ na área de Química Inorgânica
(2009).
10. L.T. Kubota, Membro titular da Academia Brasileira de Ciências (2010).
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11. Profa. Marina Tavares: Coordenadora da série de simpósios latino americanos de eletroforese
capilar e tecnologia em microchip, LACE, em 1998 e 2005 (como co-chair) e a partir de
2009 (como chair).
12. Profa. Carla Botolli: Menção Honrosa na Área de Exatas no XVII Congresso Interno de
Iniciação Científica da UNICAMP realizado nos dias 23 e 24 de setembro de 2009 com o
trabalho “Avaliação de monolitos do tipo C18 para uso em eletrocromatografia capilar”
apresentado pela aluna Valeska Soares Aguiar.
13. Prof. Emanuel Carrilho 2010: Trabalho premiado como melhor pôster na 20a Reunião Anual
de Usuários (RAU) do Laboratório Nacional de Luz Síncrotron (LNLS), Laboratório
Nacional de Luz Síncrotron (LNLS), Campinas-SP.
Courses
1. M. O. F. Goulart, Espécies Reativas de Oxigênio e Nitrogênio: Bandidos ou Mocinhos.
Congresso Acadêmico da Universidade Federal de Alagoas – 10/2009. 04 horas.
2. L. T. Kubota, W. A. Alves, Química de Biomoléculas Redox Aplicadas ao Desenvolvimento
de Biossensores, 32ª. Reunião Anual da Sociedade Brasileira – 32ªRASBQ, 30 de maio a 02
de junho de 2009, Fortaleza, CE.
3. M.O.F. Goulart, Estrategias Electroquimicas para el Estudio Del Estrés Oxidativo: Estado
del Arte y Retos em la Investigacion”, no Centro de Investigación y Desarrollo Tecnológico
em Electroquímica, em Querétaro, e no Centro de Investigación y de Estudios Avanzados CINVESTAV, na cidade do México. 10 horas/curso.
4. L.T. Kubota, Biossensores, no 7th CIFARP, Ribeirão Preto, SP, 06-09-2009. 04 horas.
5. L.T. Kubota, Biossensores, durante o I Congresso Analítica Latin América, São Paulo, SP,
08-09-2009. 02 horas.
6. L.T. Kubota, Biossensores, durante o 15º. ENQA, Salvador, BA, Brasil em 18-10-2009. 08
horas.
Invited conferences
1. W. A. Alves, Química Bioinorgânica Supramolecular Aplicada ao Desenvolvimento de
Biossensores, IV Workshop em Nanociências, 14 a 16 de setembro de 2009, Santa Maria,
RS.
2. W. A. Alves, Proteins Immobilization and Supramolecular Bioinorganic Chemistry, IV
International Symposium on Advanced Materials and Nanostructures, 17 a 19 de maio de
2009, Santo André, SP.
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3. W. A. Alves, Química Bioinorgânica Supramolecular Aplicada ao Desenvolvimento de
Biossensores, Seminários Gerais do Programa de Pós-Graduação em Química do IQ/USP, 21
de outubro de 2009, São Paulo, SP.
4. M.O.F. Goulart, PharmacoElectrochemistry. A possible interface – CINVESTAV, Cidade
do México – 12de setembro de 2009.
5. M.O.F. Goulart, Pharmacoelectrochemistry: a tool for the discovery of drugs and for the
investigation of biological mechanism of action. CIDETEQ-Queretaro, Mexico, 07de
setembro de 2009.
6. S.I. C. de Torresi, Plataformas nanoestruturadas para diversos dispositivos eletroquimicos
Conferencia plenária no XVII Simposio Brasileiro de Eletroquimica e Eletroanalitica..
Fortaleza, CE, Abril de 2009.
7. S.I. C. de Torresi, Nanoparticulas ou cavidades? Na procura de dispoistivos de alto
desempenho 32a Reunião anual da Sociedade Brasileira de Quimica. Foratleza CE, Maio
2009.
8. Z. Naal, Biossensores. Palestra proferida na IV Comemoração do Dia do Químico, de 18 de
Junho de 2009, IQ-UNESP – Araraquara.
9. Z. Naal, Molecular biology and rational design of proteins for biosensor application I
Workshop Nacional sobre Biossensores, de 07 a 09 de Outubro de 2009, IQ-UNESP –
Araraquara.
10. L.T. Kubota, Z. Naal, Tendências e Perspectivas na Área de Biossensores. I Workshop
Nacional sobre Biossensores, de 07 a 09 de Outubro de 2009, IQ-UNESP – Araraquara.
11. H. D. Abruña, Electrochemistry at the Nanoscale: Building Blocks and Techniques. 18 de
Novembro de 2009 na Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP.
12. L.T. Kubota, “Nanostructured materials as strategy for the development of electrochemical
abiotic biosensors”, durante o I Workshop Internacional de Nanomateriais e Materiais
Estruturados, Campinas, SP, Brasil, em 09-08-2009.
13. L.T. Kubota, “Molecular Imprinting as a strategy for the development of abiotic biosensors”,
durante o evento Ano da França no Brasil, São Paulo, SP, Brasil, em 14-09-2009.
14. L.T. Kubota, “Recent advances in disposable and portable biosensors”, durante o workshop
de biossensores, UNESP-Araraquara, SP, Brasil, em 09-10-2009.
15. O.Fatibello, “Electroanalytical chemistry: Where are we going?” durante o XVII SIBEE,
Fortaleza, CE, Brasil, em 19-04-2009.
58
16. O. Fatibello, “Bioelectroanalysis in Brazil”, during the Pittcon Symposium Session 1100,
Orlando, USA, 02/03/2010.
17. Carrilho, E. “Disposable Microchips for Electrophoresis and Molecular Interactions for
Detection of Biomolecules”. 7th International Congress of Pharmaceutical Sciences, Centro
de Convenções de Ribeirão Preto-SP, 06 e 07 de setembro de 2009
18. Carrilho, E. “Microfluidic Paper-Based Analytical Devices: Application In Bioassays And
Clinical Proteomics”. 3º Congresso Brasileiro de Espectrometria de Mass-BrMASS,
Campinas-SP, 12 a 15 de dezembro de 2009.
19. Carrilho, E. “Sistemas Analíticos Micro- e Nanofabricados de Baixo Custo para Bioensaios”.
15º Encontro Nacional de Química Analítica e 3º Congresso Iberoamericano de Química
Analítica, Salvador-BA, 18 a 21 de outubro de 2009.
20. Carrilho, E. “Neglected Diagnostics and Microfluidic paper-based analytical devices
(µPADs)”. Seminário: Análise de temas avançados em Química. Instituto de Química de São
Carlos, USP, São Carlos, SP, Outubro de 2009.
21. Carrilho, E. “New Platforms and New Fabrication Methods for Paper-Based Diagnostics”.
NSF Partnership in Research and Education in Materials, Harvard University, Cambridge,
MA, June 2009.
22. Carrilho, E. “Non-Aqueous Capillary Electrophoresis of Antidepressants: A Versatile Tool
From Quality Control to Therapeutic Drug Monitoring”. 15th Latin American Symposium
on Biotechnology, Biomedical, Biopharmaceutical and Industrial Applications of Capillary
Electrophoresis, and Microchip Technology (LACE 2009), CEU Universidad San Pablo,
Seville-Spain, 02 a 06 de junho de 2009.
23. Carrilho, E. “Quem disse que não existe Química Analítica em Harvard? O papel da
Química Analítica e a Química Analítica em papel”. Ciclo de Palestras e Seminários
Química às 16, Instituto de Química de São Carlos USP, São Carlos, SP, Agosto de 2009.
24. Fracassi da Silva, J. A. "Contactless Conductivity detection on microchip platforms: From
small ions to biomolecular interactions", Lab Automation 2009, january 25-28, Palm
Springs, USA.
25. Fracassi da Silva, J. A. "Detecção condutométrica sem contato em microchips: De íons
pequenos a interações Biomoleculares" - Universidade Federal de Santa Maria, 17/03/2009,
Santa Maria, RS.
26. Fracassi da Silva, J. A. "Microssistemas de Análise: Novas Fronteiras para a Análise
Química", II Semana Integrada de Química, 23/09/2009, Universidade Estadual de Mato
Grosso do Sul (UEMS) / Universidade Federal da Grande Dourados (UFGD), Dourados, MS.
59
27. Fracassi da Silva, J. A. "Contactless Conductivity detection on microchip platforms: From
small ions to biomolecular interactions", The University of Kansas, Malott Hall, February 2,
2009, Lawrence, KS, USA.
28. Medeiros, R.H.; Simionato, A.V.C. “Otimização de um método de separação de
nucleosídeos modificados e não modificados, biomarcadores tumorais, por HPLC-DAD”. I
Congresso Analítica, São Paulo – SP
29. Simionato, A. V. C.; Carrilho, E.; Tavares, M. F. M. “CE-ESI-MS: a versatile tool for the
characterization and quantitation of protein hydrolysates from different sources”. III
Congresso da Sociedade Brasileira de Espectrometria de Massas – Campinas – SP
30. Tavares, M. F. M. “Eletroforese Capilar sem fronteiras”, IQ-USP, Novembro de 2009,
31. Tavares, M. F. M. “Eletroforese Capilar sem fronteiras” Universidade Federal de Juiz de
Fora (UFJF), Juiz de Fora, MG, Fevereiro 2010.
32. Tavares, M. F. M. “Eletroforese Capilar: aplicações em alimentos” III Encontro do Instituto
Adolfo Lutz, 19 a 22 de outubro de 2009, Centro de Convenções Rebouças, São Paulo/SP,
33. Tavares, M. F. M. “Assessment of solute micelle interactions in MEKC”, LACE 2009,
Outubro 3-6, 2009, Seville, Spain.
34. Tavares, M. F. M. “The Laboratory of Chromatography and Capillary Electrophoresis: an
overview”, April 2009, Universidad San Pablo-CEU, Madrid, Spain,
35. Tavares, M. F. M., Curso: “Eletroforese Capilar: fundamentos e aplicações representativas”
Abril 2009, Patrocinador: POLIMATE, IQ-USP.
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