Evaluation Report - 2008 CIMAR_Evaluation_Report_2008

Transcrição

ACTIVITY
REPORT 2008
Content: Annual Activity Report 2008, presented by CIMAR – Associated Laboratory to
Fundação para a Ciência e a Tecnologia.
LIST OF CONTENTS
1. GENERAL INFORMATION ....................................................................................................1
2. RESEARCH TEAM ................................................................................................................2
3. OBJECTIVES AND ACHIEVEMENTS ..................................................................................... 12
4. ACTIVITIES ....................................................................................................................... 15
5. FUNDING ......................................................................................................................... 17
6. GENERAL INDICATORS ...................................................................................................... 18
7. RESEARCH LINES (THEMATIC AREAS)................................................................................. 20
RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems ...................................... 20
RL2 - Environmental Chemistry and Toxicology........................................................................ 27
RL3 - Biology and Marine Biotechnologies................................................................................ 34
RL4 - Aquaculture ...................................................................................................................... 40
8. RESEARCH GROUPS .......................................................................................................... 45
Aquaculture Research Group (Aquagroup) ............................................................................... 47
Biodiversity of Aquatic Ecosystems........................................................................................... 58
Biogeographical Ecology and Evolution (BEE) ........................................................................... 67
Biophysics .................................................................................................................................. 75
Biotechnology and Molecular Biology of Microalgae (BMBM) ................................................. 80
BioVanadium ............................................................................................................................. 86
Cellular and Molecular Studies (LECEM) ................................................................................... 90
Chemical Speciation and Bioavailability (CS&B) ....................................................................... 97
Comparative Molecular Endocrinology (CME) ........................................................................ 106
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH) .............................. 116
Ecophysiology.......................................................................................................................... 122
Ecotoxicology (ECOTOX) .......................................................................................................... 130
Ecotoxicology, Genomics and Evolution (LEGE) ...................................................................... 140
Environmental Technologies ................................................................................................... 148
Environmental Toxicology ....................................................................................................... 154
Evolution, Development and Gene Expression (EDGE)........................................................... 162
Fish Nutrition........................................................................................................................... 174
Fisheries Biology and Hydroecology Research Group (FBHRG) .............................................. 181
Fisheries, Biodiversity and Conservation ................................................................................ 186
Geology and Paleoceanography ............................................................................................. 197
Hydrobiology ........................................................................................................................... 205
Marine Plant Ecology Research Group (ALGAE)...................................................................... 212
Nutrition, Growth and Quality of Fish (LANUCE) .................................................................... 220
Oceanic and Coastal Dynamics ............................................................................................... 228
Pathology ................................................................................................................................ 235
Synthesis and Organic Reactivity ............................................................................................ 242
9. OTHER LA ACTIVITIES...................................................................................................... 246
9a. Services and resources ...................................................................................................... 246
9b. Networking actions ........................................................................................................... 249
9c. Training .............................................................................................................................. 250
9d. Outreach/science and society ........................................................................................... 251
9e. International events .......................................................................................................... 253
10. INTERNAL EVALUATIONS .............................................................................................. 255
11. FUTURE OBJECTIVES ..................................................................................................... 256
SCIENTIFIC OUTPUT 2008 (Publications, Advanced Training, Communications, Patents)
Evaluation Report 2008
1. GENERAL INFORMATION
1.1 Name of Research Unit
CIMAR - Centro de Investigação Marinha e Ambiental
1.2 Coordinator
João José Oliveira Dias Coimbra
1.3 Main Scientific Domain
Marine Sciences
1.4 Leading Hosting Institution
Centro Interdisciplinar de Investigação Marinha e Ambiental - CIIMAR
1.5 Other Insitutions involved
Centro de Ciências do Mar - CCMAR
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2. RESEARCH TEAM
2.1 Member researchers with PhD
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2
1. Adelino Vicente Mendonca Canario (Agregação / Professor Catedrático / 50%)
2. Aires Manuel Pereira Oliva Teles (Agregação / Professor Catedrático / 50%)
3. Anake Kijjoa (Agregação / Professor Catedrático / 60%)
4. Deborah Mary Power (Agregação / Professor Catedrático / 50%)
5. Eduardo Jorge Sousa Rocha (Agregação / Professor Catedrático / 40%)
6. Emidio Ferreira Santos Gomes (Agregação / Professor Catedrático / 65%)
7. Fernando Francisco Machado Veloso Gomes (Agregação / Professor Catedrático / 60%)
8. João José Oliveira Dias Coimbra (Agregação / Professor Catedrático / 70%)
9. Jorge Guimaraes da Costa Eiras (Agregação / Professor Catedrático / 50%)
10. José Pedro de Andrade e Silva Andrade (Agregação / Professor Catedrático / 50%)
11. Lúcia Maria das Candeias Guilhermino (Agregação / Professor Catedrático / 60%)
12. Maria Armanda Reis Henriques (Agregação / Professor Catedrático / 50%)
13. Maria Leonor Quintais Cancela Fonseca (Agregação / Professor Catedrático / 50%)
14. Maria Teresa Sa Dias de Vasconcelos (Agregação / Professor Catedrático / 40%)
15. Rogério Alves Ferreira Monteiro (Agregação / Professor Catedrático / 40%)
16. Vitor Manuel Oliveira Vasconcelos (Agregação / Professor Associado / 70%)
17. Adriano Agostinho Donas Bôto Bordalo e Sá (Agregação / Professor Associado / 40%)
18. Alexandre Manuel Silva Lobo Cunha (Agregação / Professor Associado / 40%)
19. António José Rogeiro Gouveia (Agregação / Professor Associado / 50%)
20. Francisco de Almeida Taveira Pinto (Agregação / Professor Associado / 50%)
21. Maria Leonor Fidalgo (Agregação / Professor Associado / 50%)
22. Maria Lurdes Santos Cristiano (Agregação / Professor Associado / 50%)
23. Maria Margarida M Castro (Agregação / Professor Associado / 50%)
24. Rui Orlando Pimenta Santos (Agregação / Professor Associado / 50%)
25. Ana Isabel de Melo Azevedo Neto (Agregação / Professor Auxiliar / 25%)
26. Aurélia Maria de Pinho Marques Saraiva (Agregação / Professor Auxiliar / 50%)
27. Maria Natividade Ribeiro Vieira (Agregação / Professor Auxiliar / 50%)
28. Paulo José de Azevedo Pinto Rema (Agregação / Professor Auxiliar / 50%)
29. Peter Wirtz (Agregação / Investigador / 50%)
30. Carlos José Correia de Azevedo (Agregação / Não aplicável (aposentado) / 80%)
31. Maria Teresa Dinis (Agregação / Não aplicável (aposentado) / 100%)
32. António Manuel Santos Afonso (Doutoramento / Professor Associado / 70%)
33. Fernanda Russell Pinto (Doutoramento / Professor Associado / 60%)
34. João Paulo de Sousa Cabral (Doutoramento / Professor Associado / 35%)
35. Jorge Pereira Machado (Doutoramento / Professor Associado / 50%)
36. Karim Erzini (Doutoramento / Professor Associado / 50%)
37. Luis Manuel Zambujal Chicharo (Doutoramento / Professor Associado / 50%)
38. Luísa Maria Pinheiro Valente (Doutoramento / Professor Associado / 75%)
39. Manuel Aureliano Pereira Martins Alves (Doutoramento / Professor Associado / 50%)
40. Maria Alexandra Anica Teodosio Chicharo (Doutoramento / Professor Associado / 50%)
41. Maria Joao Tome Costa Sousa Rocha (Doutoramento / Professor Associado / 40%)
42. Maria Leonor Nunes Ribeiro Cruzeiro (Doutoramento / Professor Associado / 50%)
43. Paulo Manuel Rodrigues Vaz-Pires (Doutoramento / Professor Associado / 50%)
44. Alberto Teodorico Rodrigues Moura Correia (Doutoramento / Professor Auxiliar / 50%)
45. Ana Carolina Tavares Estima Santos da Cunha Feming (Doutoramento / Professor Auxiliar /
40%)
46. António Paulo Alves Ferreira de Carvalho (Doutoramento / Professor Auxiliar / 50%)
47. Carla Batista Carvalho Batista Pinto (Doutoramento / Professor Auxiliar / 25%)
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48. Cristina Maria Bravo de Faria Cruz (Doutoramento / Professor Auxiliar / 50%)
49. Dina Cristina Fernandes Rodrigues da Costa Simes (Doutoramento / Professor Auxiliar / 50%)
50. Eduardo Nuno Picoto Lopes Barata (Doutoramento / Professor Auxiliar / 50%)
51. Gerhard Michael Weber (Doutoramento / Professor Auxiliar / 30%)
52. Isabel Maria Trigueiros Sousa Pinto Machado (Doutoramento / Professor Auxiliar / 50%)
53. João Carlos Serafim Varela (Doutoramento / Professor Auxiliar / 50%)
54. José Américo Pereira de Sousa (Doutoramento / Professor Auxiliar / 50%)
55. Jose Carlos Fernandes Antunes (Doutoramento / Professor Auxiliar / 20%)
56. José Fernando Magalhães Gonçalves (Doutoramento / Professor Auxiliar / 30%)
57. Luis Filipe Pereira de Oliva Teles (Doutoramento / Professor Auxiliar / 50%)
58. Luisa Paula Viola Afonso Barreira (Doutoramento / Professor Auxiliar / 50%)
59. Manfred Josef Kaufmann (Doutoramento / Professor Auxiliar / 40%)
60. Margarida de Lurdes de Jesus Bastos Cristo (Doutoramento / Professor Auxiliar / 50%)
61. Maria Antonia Santos Mendes Salgado (Doutoramento / Professor Auxiliar / 35%)
62. Maria Clara Ramalho Monteiro Pires Basto (Doutoramento / Professor Auxiliar / 50%)
63. Maria Clara Semedo da Silva Costa (Doutoramento / Professor Auxiliar / 40%)
64. Maria Ester Tavares Alvares Serrao (Doutoramento / Professor Auxiliar / 50%)
65. Maria João Faria Leite Dias dos Santos (Doutoramento / Professor Auxiliar / 50%)
66. Maria Teresa Calvinho Cerveira Borges (Doutoramento / Professor Auxiliar / 50%)
67. Maria Teresa Martins Borges (Doutoramento / Professor Auxiliar / 40%)
68. Nuno Miguel Pinto de Sousa Monteiro (Doutoramento / Professor Auxiliar / 30%)
69. Olga Maria Oliveira da Silva Lage (Doutoramento / Professor Auxiliar / 40%)
70. Paula Cristina Paulo Videira da Silva (Doutoramento / Professor Auxiliar / 40%)
71. Paulo Alexandre de Avilez Rodrigues de Almeida Valente (Doutoramento / Professor Auxiliar /
60%)
72. Paulo Jose Talhadas Santos (Doutoramento / Professor Auxiliar / 50%)
73. Paulo Manuel Rodrigues Martins da Costa (Doutoramento / Professor Auxiliar / 50%)
74. Pedro Miguel Leal Rodrigues (Doutoramento / Professor Auxiliar / 50%)
75. Rita Castilho (Doutoramento / Professor Auxiliar / 50%)
76. Sergio Reis Cunha (Doutoramento / Professor Auxiliar / 30%)
77. Teresa Isabel Mendonca Modesto (Doutoramento / Professor Auxiliar / 50%)
78. Albina Dolores Cardoso da Silva Castro Resende (Doutoramento / Professor Coordenador /
25%)
79. Eduardo Bruno Oliveira Esteves (Doutoramento / Professor-Adjunto Equiparado / 50%)
80. Maria Luisa Machado Cerqueira Bastos (Doutoramento / Investigador Principal / 50%)
81. Agostinho Antunes Pereira (Doutoramento / Investigador Auxiliar / 100%)
82. Alexandre Marmoto de Oliveira Campos (Doutoramento / Investigador Auxiliar / 100%)
83. Ana Maria Ferreira Bio (Doutoramento / Investigador Auxiliar / 100%)
84. Ana Paula de Campos Mucha (Doutoramento / Investigador Auxiliar / 100%)
85. Carlos Alexandre Sarabando Gravato (Doutoramento / Investigador Auxiliar / 100%)
86. Channarayapattana Narasimhamurthy Prabhu (Doutoramento / Investigador Auxiliar / 100%)
87. Cristina Marisa Ribeiro de Almeida (Doutoramento / Investigador Auxiliar / 100%)
88. Cymon John Cox (Doutoramento / Investigador Auxiliar / 100%)
89. Filipe Alexandre Oliveira dos Santos Alberto (Doutoramento / Investigador Auxiliar / 100%)
90. Francisco Arenas Parra (Doutoramento / Investigador Auxiliar / 100%)
91. Gareth Anthony Pearson (Doutoramento / Investigador Auxiliar / 100%)
92. Isabel Gonçalves de Barbosa Araújo (Doutoramento / Investigador Auxiliar / 100%)
93. João Carlos dos Reis Cardoso (Doutoramento / Investigador Auxiliar / 100%)
94. João Miguel Sousa da Silva (Doutoramento / Investigador Auxiliar / 100%)
95. Jonathan Mark Wilson (Doutoramento / Investigador Auxiliar / 100%)
96. Jorge Manuel dos Santos Goncalves (Doutoramento / Investigador Auxiliar / 100%)
97. Jorge Proença Dias (Doutoramento / Investigador Auxiliar / 100%)
98. Juan Fuentes Diaz (Doutoramento / Investigador Auxiliar / 100%)
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99. Laura Maria Simões Coutinho Guimarães (Doutoramento / Investigador Auxiliar / 100%)
100. Luis Eugenio Castanheira Da Conceicao (Doutoramento / Investigador Auxiliar / 100%)
101. Luís Filipe Costa Castro (Doutoramento / Investigador Auxiliar / 100%)
102. Machiel Simon Bos (Doutoramento / Não aplicável (bolseiro) / 100%)
103. Maria Helena Tabuaço Rêgo Martins Peres (Doutoramento / Investigador Auxiliar / 100%)
104. Miguel Alberto Fernandes Machado e Santos (Doutoramento / Investigador Auxiliar / 100%)
105. Paulo Jorge Travessa Gavaia (Doutoramento / Investigador Auxiliar / 100%)
106. Pedro Miguel Guerreiro Costa Guerreiro (Doutoramento / Investigador Auxiliar / 100%)
107. Peter Colin Hubbard (Doutoramento / Investigador Auxiliar / 100%)
108. Radhouane Ben Hamadou (Doutoramento / Investigador Auxiliar / 100%)
109. Ralph Urbatzka (Doutoramento / Investigador Auxiliar / 100%)
110. Rodrigo da Silva Costa (Doutoramento / Investigador Auxiliar / 100%)
111. Rodrigo Ozorio (Doutoramento / Investigador Auxiliar / 100%)
112. Rui Miguel Andrade Caldeira (Doutoramento / Investigador Auxiliar / 100%)
113. Sophie Arnaud-Haond (Doutoramento / Investigador Auxiliar / 100%)
114. Susana Maria Pereira da Costa Moreira (Doutoramento / Investigador Auxiliar / 100%)
115. Sven Wuertz (Doutoramento / Investigador Auxiliar / 100%)
116. Vicent Laizé (Doutoramento / Investigador Auxiliar / 100%)
117. Ana Maria de Melo Sampaio de Freitas (Doutoramento / Investigador / 50%)
118. Carla Maria Abreu Freitas (Doutoramento / Investigador / 20%)
119. Cláudia Maria Neves Delgado (Doutoramento / Investigador / 25%)
120. Erik-jan Malta (Doutoramento / Investigador / 100%)
121. João Lemos Gomes Canning Clode (Doutoramento / Investigador / 100%)
122. Maria Begona Redruello Trelles (Doutoramento / Investigador / 100%)
123. Maria Dulce da Mota Antunes de Oliveira Estêvão (Doutoramento / Investigador / 40%)
124. Maria Isabel da Silva Costa Azevedo (Doutoramento / Investigador / 50%)
125. Rosa Carmina Cervantes Cianca (Doutoramento / Investigador / 50%)
126. Maria do Rosário Fidalgo Martins (Doutoramento / Assistente / 40%)
127. Alexandra Paula Mimoso Henriques Cunha (Doutoramento / Não aplicável (bolseiro) /
100%)
128. Alfredo Nuno Damasceno Pinto de Oliveira (Doutoramento / Não aplicável (bolseiro) /
100%)
129. Ana Luisa Frazão Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
130. Ana Mafalda Baptista Saraiva (Doutoramento / Não aplicável (bolseiro) / 100%)
131. António de Vilhena Andrade Ferreira Sykes (Doutoramento / Não aplicável (bolseiro) /
100%)
132. Arnau Rodriguez Illamola (Doutoramento / Não aplicável (bolseiro) / 100%)
133. Aschwin Hillebrand Engelen (Doutoramento / Não aplicável (bolseiro) / 100%)
134. Carina Santos da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)
135. Catarina Isabel de Matos Martins (Doutoramento / Não aplicável (bolseiro) / 100%)
136. Catarina Maria Pinto Mora Pinto de Magalhães - (Doutoramento / Não aplicável (bolseiro) /
100%)
137. Christophe Haond (Doutoramento / Não aplicável (bolseiro) / 100%)
138. Cláudia Raquel Cêa de Aragão Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%)
139. Cristina Isabel Coelho Dias Lopes (Doutoramento / Não aplicável (bolseiro) / 100%)
140. Custódia Maria Luís Gago (Doutoramento / Não aplicável (bolseiro) / 100%)
141. Daniel António Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%)
142. Dulce Isabel Assis Alves Martins (Doutoramento / Não aplicável (bolseiro) / 100%)
143. Edite Manuela da Graça Veríssimo (Doutoramento / Não aplicável (bolseiro) / 100%)
144. Elsa Alexandra Martins Silva Cabrita (Doutoramento / Não aplicável (bolseiro) / 100%)
145. Fernando Cánivas García (Doutoramento / Não aplicável (bolseiro) / 100%)
146. Florbela Maria Benjamim Soares (Doutoramento / Não aplicável (bolseiro) / 100%)
147. Isabel Cristina Guimarães Nogueira (Doutoramento / Não aplicável (bolseiro) / 100%)
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148. Isabel Cristina Oliveira Gonzalez Cunha (Doutoramento / Não aplicável (bolseiro) / 100%)
149. Isabel Martins Gil (Doutoramento / Não aplicável (bolseiro) / 100%)
150. Joana Fernades da Fonseca da Costa Martins Osswald (Doutoramento / Não aplicável
(bolseiro) / 100%)
151. Joana Ferreira Marques Ferreira Cardoso (Doutoramento / Não aplicável (bolseiro) / 100%)
152. Jorge Afonso Martins da Palma (Doutoramento / Não aplicável (bolseiro) / 100%)
153. José Carlos Caetano Xavier (Doutoramento / Não aplicável (bolseiro) / 100%)
154. Justyna Kopecka-Pilarczyk (Doutoramento / Não aplicável (bolseiro) / 100%)
155. Katy Nicastro (Doutoramento / Não aplicável (bolseiro) / 100%)
156. Laurence Alexandrine Mathilde Deloffre (Doutoramento / Não aplicável (bolseiro) / 100%)
157. Ledicia Rey Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%)
158. Leonardo Filipe Rodrigues da Mata (Doutoramento / Não aplicável (bolseiro) / 100%)
159. Manuel Gesto Rodríguez (Doutoramento / Não aplicável (bolseiro) / 100%)
160. Marcos Rubal Garcia (Doutoramento / Não aplicável (bolseiro) / 100%)
161. María Asunción Lago Lestón (Doutoramento / Não aplicável (bolseiro) / 100%)
162. Maria del Mar Huertas Pau (Doutoramento / Não aplicável (bolseiro) / 100%)
163. Maria Elena Varela Alvarez (Doutoramento / Não aplicável (bolseiro) / 100%)
164. Maria Emília Carvalho Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%)
165. Maria Laura Braga Ribeiro (Doutoramento / Não aplicável (bolseiro) / 100%)
166. Marta Sofia Sa Ferreira (Doutoramento / Não aplicável (bolseiro) / 100%)
167. Nadège Richard (Doutoramento / Não aplicável (bolseiro) / 100%)
168. Natércia Maria da Silva Conceição (Doutoramento / Não aplicável (bolseiro) / 100%)
169. Nuna Cláudia Peixoto de Araújo (Doutoramento / Não aplicável (bolseiro) / 100%)
170. Onno Everhardus Diekmann (Doutoramento / Não aplicável (bolseiro) / 100%)
171. Patrícia Isabel Silvestre Pinto (Doutoramento / Não aplicável (bolseiro) / 100%)
172. Paula Cristina Enes Oliveira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)
173. Pedro Alexandre Garcia Range (Doutoramento / Não aplicável (bolseiro) / 100%)
174. Pedro Miguel Coutinho Victorino Borges Morais (Doutoramento / Não aplicável (bolseiro) /
100%)
175. Pedro Miguel Lopes Gonçalves (Doutoramento / Não aplicável (bolseiro) / 100%)
176. Rawiwan Watanadilok (Doutoramento / Não aplicável (bolseiro) / 100%)
177. Rita Alexandra Duarte Borges (Doutoramento / Não aplicável (bolseiro) / 100%)
178. Ronaldo Gomes de Sousa (Doutoramento / Não aplicável (bolseiro) / 100%)
179. Rui Manuel do Amaral Branco de Oliveira Quartau (Doutoramento / Não aplicável (bolseiro)
/ 100%)
180. Rui Pedro Andrade Coelho (Doutoramento / Não aplicável (bolseiro) / 100%)
181. Rui Pedro Goncalves Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
182. Rute Andreia Rodrigues da Fonseca (Doutoramento / Não aplicável (bolseiro) / 100%)
183. Rute Sofia Tavares Martins Brazona (Doutoramento / Não aplicável (bolseiro) / 100%)
184. Sandra Cristina da Costa e Silva Ramos (Doutoramento / Não aplicável (bolseiro) / 100%)
185. Sara Judite Lopes Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%)
186. Sara Maria Mira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)
187. Sofia Alexandra Dias Engrola (Doutoramento / Não aplicável (bolseiro) / 100%)
188. Stefano Vaselli (Doutoramento / Não aplicável (bolseiro) / 100%)
189. Susana Isabel dos Santos Cabaço (Doutoramento / Não aplicável (bolseiro) / 100%)
190. Susana Rodrigues Pereira (Doutoramento / Não aplicável (bolseiro) / 100%)
191. Teodor Ludmilov Stoichev (Doutoramento / Não aplicável (bolseiro) / 100%)
192. Teresa Paula Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%)
193. Tida Dethoup (Doutoramento / Não aplicável (bolseiro) / 100%)
194. Vanessa Schein (Doutoramento / Não aplicável (bolseiro) / 100%)
195. Vijayakumar Parameswaran (Doutoramento / Não aplicável (bolseiro) / 100%)
196. Zardi Gerardo (Doutoramento / Não aplicável (bolseiro) / 100%)
197. Carlos Gil Ribeiro Martins (Doutoramento / Técnico Superior e Técnico / 100%)
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198. Cláudia Correia da Silva Ribeiro (Doutoramento / Técnico Superior e Técnico / 80%)
199. Paula Raquel da Silva Jorge Coutinho Ferreira (Doutoramento / Técnico Superior e Técnico /
100%)
2.2 Member researchers without PhD
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1. Agnès Marhadour (Mestrado / Não aplicável (bolseiro) / 100%)
2. Amélia Cláudia Figueiredo Silva (Mestrado / Não aplicável (bolseiro) / 100%)
3. Ana Isabel Delfin dos Santos Alexandre (Mestrado / Não aplicável (bolseiro) / 100%)
4. Ana Mafalda Rocha Tavares (Mestrado / Não aplicável (bolseiro) / 100%)
5. António José Mendes Martins (Mestrado / Não aplicável (bolseiro) / 100%)
6. Benjamín Costas Refojos (Mestrado / Não aplicável (bolseiro) / 100%)
7. Carla Alexandra da Silva Monteiro (Mestrado / Não aplicável (bolseiro) / 100%)
8. Catarina Fernanda de Carvalho Pinheiro Teixeira (Mestrado / Não aplicável (bolseiro) / 100%)
9. Catarina Figueiredo da Mota (Mestrado / Não aplicável (bolseiro) / 100%)
10. Chommanard Sumngern (Mestrado / Não aplicável (bolseiro) / 100%)
11. Claire Juliana Francisco (Mestrado / Não aplicável (bolseiro) / 100%)
12. Cristiana Ivone Tavares Moreira (Mestrado / Não aplicável (bolseiro) / 100%)
13. Cristina Maria Simões de Jesus Rocha (Mestrado / Não aplicável (bolseiro) / 100%)
14. David Maria Aguiar Abecasis (Mestrado / Não aplicável (bolseiro) / 100%)
15. David Veríssimo Piló (Mestrado / Não aplicável (bolseiro) / 100%)
16. Diogo Filipe da Silva Gonçalves Soares Paulo (Mestrado / Não aplicável (bolseiro) / 100%)
17. Fátima de Carvalho Vaz Pinto (Mestrado / Não aplicável (bolseiro) / 100%)
18. Guillermin Aguero Chapin (Mestrado / Não aplicável (bolseiro) / 100%)
19. Inês Costa de Carvalho (Mestrado / Não aplicável (bolseiro) / 100%)
20. Joana Ferreira Costa (Mestrado / Não aplicável (bolseiro) / 100%)
21. Joana Isabel Correia Bondoso (Mestrado / Não aplicável (bolseiro) / 100%)
22. Joana Margarida Guimarães e Silva (Mestrado / Não aplicável (bolseiro) / 100%)
23. Joana Maria Reis Franco Cruz (Mestrado / Não aplicável (bolseiro) / 100%)
24. José Carlos de Morais Martins (Mestrado / Não aplicável (bolseiro) / 100%)
25. Leonor Isabel Moreira de Araújo (Mestrado / Não aplicável (bolseiro) / 100%)
26. Luciana Paiva das Neves (Mestrado / Não aplicável (bolseiro) / 100%)
27. Luis Miguel dos Santos Russo Vieira (Mestrado / Não aplicável (bolseiro) / 100%)
28. Mafalda Rangel Malheiro Dias de Oliveira (Mestrado / Não aplicável (bolseiro) / 100%)
29. Mahaut Diane Marie Stephanie de Labroue de Vareilles Sommieres (Mestrado / Não aplicável
(bolseiro) / 100%)
30. Maria Catarina Murteira Rico dos Santos Campos (Mestrado / Não aplicável (bolseiro) /
100%)
31. Mariana Fonseca Hinzmann (Mestrado / Não aplicável (bolseiro) / 100%)
32. Miguel Filipe Ferreira da Costa Ramos (Mestrado / Não aplicável (bolseiro) / 100%)
33. Miguel José Teodoro Correia (Mestrado / Não aplicável (bolseiro) / 100%)
34. Mirjam Susanne van de Vliet (Mestrado / Não aplicável (bolseiro) / 100%)
35. Monya Mendes Costa (Mestrado / Não aplicável (bolseiro) / 100%)
36. Odete Marinho Gonçalves (Mestrado / Não aplicável (bolseiro) / 100%)
37. Paulo Alexandre dos Santos Silva (Mestrado / Não aplicável (bolseiro) / 100%)
38. Pedro Alexandrino Silva Ferreira Martins Monteiro (Mestrado / Não aplicável (bolseiro) /
100%)
39. Pedro Fernandes Seixas (Mestrado / Não aplicável (bolseiro) / 100%)
40. Pedro Neves de Carvalho (Mestrado / Não aplicável (bolseiro) / 100%)
41. Pedro Nuno da Costa Leão (Mestrado / Não aplicável (bolseiro) / 100%)
42. Ricardo Manuel Rafael Afonso (Mestrado / Não aplicável (bolseiro) / 100%)
43. Ricardo Mario Bastos Leite (Mestrado / Não aplicável (bolseiro) / 100%)
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44. Richard Deurloo (Mestrado / Não aplicável (bolseiro) / 100%)
45. Rita Micaela dos Santos Fernandes de Araujo (Mestrado / Não aplicável (bolseiro) / 100%)
46. Sofia Gabriel Garcia Santos (Mestrado / Não aplicável (bolseiro) / 100%)
47. Sonia Maria de Sousa Olim (Mestrado / Não aplicável (bolseiro) / 100%)
48. Susana Maria Rocha Pina (Mestrado / Não aplicável (bolseiro) / 100%)
49. Tânia Ribeiro Pereira (Mestrado / Não aplicável (bolseiro) / 100%)
50. Vânia Cristina Palma Roberto (Mestrado / Não aplicável (bolseiro) / 100%)
51. Vasco Manuel Nobre de Carvalho da Silva Vieira (Mestrado / Não aplicável (bolseiro) / 100%)
52. Vera Alexandra Garcia da Fonseca Batista (Mestrado / Não aplicável (bolseiro) / 100%)
53. Joana do Passo Carneiro Azevedo (Mestrado / Apoio Técnico/ 100%)
54. Marina N’Deye Pereira Silva (Mestrado / Apoio Técnico / 50%)
55. Susana Micaela Machado Ferreira do Vale (Mestrado / Apoio Técnico/ 100%)
56. Ana Sousa Ramos Ramalho Ribeiro de Magalhães Sant’Ana (Mestrado / Técnico Superior e
Técnico/ 100%)
57. Beatriz Maria Alvega Cardoso (Mestrado / Técnico Superior e Técnico/ 100%)
58. Cristina Maria Viegas Inácio (Mestrado / Técnico Superior e Técnico/ 100%)
59. Emília da Glória Moreira Afonso (Mestrado / Técnico Superior e Técnico/ 100%)
60. Hugo Gabriel Maia da Silva Santos (Mestrado / Técnico Superior e Técnico/ 100%)
61. Pedro Alexandre Pereira dos Reis (Mestrado / Técnico Superior e Técnico/ 100%)
62. Garça Maria Figueiredo Casal (Licenciatura / Assistente / 50%)
63. Ana Alexandra Pedrosa Ramos (Licenciatura / Não aplicável (bolseiro) / 100%)
64. Ana Cristina da Silva Gomes (Licenciatura / Não aplicável (bolseiro) / 100%)
65. Ana Cristina Silva Rocha (Licenciatura / Não aplicável (bolseiro) / 100%)
66. Ana Filipa dos Santos Gonçalves (Licenciatura / Não aplicável (bolseiro) / 100%)
67. Ana Isabel Santos Couto (Licenciatura / Não aplicável (bolseiro) / 100%)
68. Ana Rita Andrade Coelho (Licenciatura / Não aplicável (bolseiro) / 100%)
69. Ana Rita de Araujo Ribeiro (Licenciatura / Não aplicável (bolseiro) / 100%)
70. Ana Rita de Sousa Gonçalves Costa (Licenciatura / Não aplicável (bolseiro) / 100%)
71. Anabela Ben-Simon Brito (Licenciatura / Não aplicável (bolseiro) / 100%)
72. Anabela Rodrigues Lopes (Licenciatura / Não aplicável (bolseiro) / 100%)
73. André Gonçalo Antunes dos Santos (Licenciatura / Não aplicável (bolseiro) 100%)
74. Andreia Cristina Domingues Bringela (Licenciatura / Não aplicável (bolseiro) / 100%)
75. Andreia Monteiro da Cruz Ribeiro Gouveia (Licenciatura / Não aplicável (bolseiro) / 100%)
76. Andreia Seia Rebotim (Licenciatura / Não aplicável (bolseiro) / 100%)
77. Ângela Alexandra Martinho Ramos (Licenciatura / Não aplicável (bolseiro) / 100%)
78. Brigite Sandra Nunes Simões (Licenciatura / Não aplicável (bolseiro) / 100%)
79. Bruno Emanuel Pereira Louro (Licenciatura / Não aplicável (bolseiro) / 100%)
80. Carla Alexandra São Bento Viegas (Licenciatura / Não aplicável (bolseiro) / 100%)
81. Carlos Manuel Loureco Afonso (Licenciatura / Não aplicável (bolseiro) / 100 %)
82. Carmen Sofia Cardoso Mateus (Licenciatura / Não aplicável (bolseiro) / 100%)
83. Catarina Dinis Cavaleiro (Licenciatura / Não aplicável (bolseiro) / 100%)
84. Cátia Andreia Lourenço Marques (Licenciatura / Não aplicável (bolseiro) / 100%)
85. Célia Cristina de Aguiar Carvalho (Licenciatura / Não aplicável (bolseiro) / 100%)
86. Célia Teresa Neto dos Santos (Licenciatura / Não aplicável (bolseiro) / 100%)
87. Cindy Vitória Fazenda (Licenciatura / Não aplicável (bolseiro) / 100%)
88. Claudia de Fátima Escórcio Pereira (Licenciatura / Outra / 100%)
89. Claudia Maria Rosa Ribeiro (Licenciatura / Não aplicável (bolseiro) / 100%)
90. Cláudia Sofia Patrão Beliz Rosa e Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
91. Daniela da Silva Lima (Licenciatura / Não aplicável (bolseiro) / 100%)
92. Eduardo Bruno de Freitas Vivas (Licenciatura / Não aplicável (bolseiro) / 100%)
93. Elisabete Alexandra Dias de Matos (Licenciatura / Não aplicável (bolseiro) / 100%)
94. Filipa Margarida Barroso Ferreira (Mestrado / Não aplicável (bolseiro) / 100%)
95. Florbela Alexandra Silva Vieira Martins (Licenciatura / Não aplicável (bolseiro) / 100%)
7
-
8
96. Frederico Miguel Cunha de Oliveira (Licenciatura / Não aplicável (bolseiro) / 100%)
97. Gonçalo Jorge Franco Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
98. Helena Rita de Carvalho Ferraz Pedrosa Teodósio (Licenciatura / Não aplicável (bolseiro) /
100%)
99. Henrique Miguel Morais Folhas (Licenciatura / Não aplicável (bolseiro) / 100%)
100. Joana Alexandra Teixeira Rosa (Licenciatura / Não aplicável (bolseiro) / 100%)
101. Joana Catarina Rocha Moreira da Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
102. Joana Faria da Costa (Licenciatura / Não aplicável (bolseiro) / 100%)
103. Joana Reis de Almeida (Licenciatura / Não aplicável (bolseiro) / 100%)
104. Joana Silveira Soares (Licenciatura / Não aplicável (bolseiro) / 100%)
105. João Miranda Neiva (Licenciatura / Não aplicável (bolseiro) / 100%)
106. João Paulo Rodrigues Machado (Licenciatura / Não aplicável (bolseiro) / 100%)
107. João Soares Carrola (Licenciatura / Não aplicável (bolseiro) / 100%)
108. Licínia Cristina Freitas Gouveia (Licenciatura / Não aplicável (bolseiro) / 100%)
109. Lília Isabel Lameirinhas Cabral (Licenciatura / Não aplicável (bolseiro) / 100%)
110. Liliana Isabel Tome Dos Anjos Guerreiro (Licenciatura / Não aplicável (bolseiro) / 100%)
111. Luis Manuel Correia Ferreira Bentes (Licenciatura / Não aplicável (bolseiro) / 100%)
112. Manuel Peixoto de Magalhães Lopes Lima (Licenciatura / Não aplicável (bolseiro) / 100%)
113. Márcio Alexandre Filipe Simão (Licenciatura / Não aplicável (bolseiro) / 100%)
114. Maria da Nazaré Parada Figueiredo de Sousa Couto (Licenciatura / Não aplicável (bolseiro) /
100%)
115. Maria Helena Trindade de Abreu (Licenciatura / Não aplicável (bolseiro) / 100%)
116. Maria Inês de Almeida Páscoa (Licenciatura / Não aplicável (bolseiro) / 100%)
117. Maria Inês Oliveira Pinto Coelho (Licenciatura / Não aplicável (bolseiro) / 100%)
118. Maria Joao Rodrigues Pereira (Licenciatura / Não aplicável (bolseiro) / 100%)
119. Marisa Sárria Pereira de Passos (Licenciatura / Não aplicável (bolseiro) / 100%)
120. Marta Isabel da Silva Rafael (Licenciatura / Não aplicável (bolseiro) / 100%)
121. Michael Nogueira Viegas (Licenciatura / Não aplicável (bolseiro) / 100%)
122. Monica Sofia Furtado Martins (Licenciatura / Não aplicável (bolseiro) / 100%)
123. Nair Alexandra Barbosa Nazareth Campos (Licenciatura / Não aplicável (bolseiro) / 100%)
124. Nelson Alexandre Castilho Coelho (Licenciatura / Não aplicável (bolseiro) / 100%)
125. Nikolay Kolmakov (Licenciatura / Não aplicável (bolseiro) / 100%)
126. Odete Dmingues Cordeiro (Licenciatura / Não aplicável (bolseiro) / 100%)
127. Patricia Alexandra Cavaleiro Diogo (Licenciatura / Não aplicável (bolseiro) / 100%)
128. Paulo César Nunes Pereira do Rêgo (Licenciatura / Não aplicável (bolseiro) / 50%)
129. Paulo Jorge Rosa Santos (Licenciatura / Não aplicável (bolseiro) / 100%)
130. Pedro Alexandre Cardoso Matos (Licenciatura / Não aplicável (bolseiro) / 40%)
131. Pedro Alexandre Coelho Borges (Licenciatura / Não aplicável (bolseiro) / 100%)
132. Pedro Filipe dos Santos Palma (Licenciatura / Não aplicável (bolseiro) / 100%)
133. Pedro Filipe Duarte Alves da Veiga (Licenciatura / Não aplicável (bolseiro) / 100%)
134.Pedro Luís Martins de Castro Pinheiro (Licenciatura / Não aplicável (bolseiro) / 100%)
135. Pedro Miguel Quintas Barroso (Licenciatura / Não aplicável (bolseiro) / 100%)
136. Rita Alves Costa (Licenciatura / Não aplicável (bolseiro) / 100%)
137. Rita de Sousa Braga e Sá (Licenciatura / Não aplicável (bolseiro) / 100%)
138. Rita Isabel Pontes Barbosa Colen (Licenciatura / Não aplicável (bolseiro) / 100%)
139. Rui Filipe de Ascensao Almeida (Licenciatura / Não aplicável (bolseiro) / 50%)
140. Rui Tiago Aguiar Cardoso (Licenciatura / Não aplicável (bolseiro) / 20%)
141. Rute Isabel Pinto de Brito (Licenciatura / Não aplicável (bolseiro) / 100%)
142. Sandra Cláudia Matias Rodrigues (Licenciatura / Não aplicável (bolseiro) / 100%)
143. Sérgia Catarina de Amorim Costa Dias (Licenciatura / Não aplicável (bolseiro) / 100%)
144. Silvia Maria dos Santos Albano (Licenciatura / Não aplicável (bolseiro) / 100%)
145. Sónia Isabel Rodrigues Aldeia Sanches Massa (Licenciatura / Não aplicável (bolseiro) / 100%)
146. Tânia Raquel Santos Aires (Licenciatura / Não aplicável (bolseiro) / 100%)
-
147. Tânia Vieira Madureira (Licenciatura / Não aplicável (bolseiro) / 25%)
148 Tomé Pereira de Azevedo Santos Silva (Licenciatura / Não aplicável (bolseiro) / 100%)
149. Vasco Manuel Paiva Ferreira (Licenciatura / Não aplicável (bolseiro) 100%)
150. Vera Lúcia Fernandes Rodrigues (Licenciatura / Não aplicável (bolseiro) / 100%)
151. Viviana Raquel Vieira da Silva Lopes (Licenciatura / Não aplicável (bolseiro) / 100%)
152. Wilson Gabriel Poseiro Coutinho Pinto (Licenciatura / Não aplicável (bolseiro) / 100%)
153. Alexandre Filipe Leitão (Licenciatura / Não aplicável (Estudante) / 100%)
154. Ana Catarina da Cruz Gonçalves Café (Licenciatura / Não aplicável (Estudante) / 100%)
155. Ana Margarida Guerreiro Pereira (Licenciatura / Não aplicável (Estudante) / 100%)
156. Ana Margarida Pinto Henrique Machado (Licenciatura / Não aplicável (Estudante) / 100%)
157. Ana Vanessa Basto Regueiras (Licenciatura / Não aplicável (Estudante) / 100%)
158. Carolina Machado Malheiro Rodrigues (Licenciatura / Não aplicável (Estudante) / 100%)
159. Catarina da Rocha Cruzeiro (Licenciatura / Não aplicável (Estudante) / 100%)
160. Daniel João da Silva Tavares Duarte (Licenciatura / Não aplicável (Estudante) / 100%)
161. Dorabela Soares Sampaio Pereira Pinto (Licenciatura / Não aplicável (Estudante) / 100%)
162. Eva Catarina Costa Amorim (Licenciatura / Não aplicável (Estudante) / 100%)
163. Fernanda Cristina Rodrigues Malhão Pereira (Licenciatura / Não aplicável (Estudante) /
100%)
164. João Carlos Pereira Morais (Licenciatura / Não aplicável (Estudante) / 100%)
165. João da Mota e Silva Rodrigues de Carvalho (Licenciatura / Não aplicável (Estudante) /
100%)
166. Joaquim Manuel Soares Guedes (Licenciatura / Não aplicável (Estudante) / 100%)
167. José Miguel Pereira Gomes (Licenciatura / Não aplicável (Estudante) / 100%)
168. Ligia Maria Chaves da Silva (Licenciatura / Não aplicável (Estudante) / 100%)
169. Luis Gabriel da Silva Luis (Licenciatura / Não aplicável (Estudante) / 100%)
170. Maria Alexandra Santos Martins (Licenciatura / Não aplicável (Estudante) / 100%)
171. Maria de Fátima Saavedra Barroso (Licenciatura / Não aplicável (Estudante) / 100%)
172. Maria Margarida Oliveira Maló Machado (Licenciatura / Não aplicável (Estudante) / 100%)
173. Patrícia Alexandra Correia Oliveira (Licenciatura / Não aplicável (Estudante) / 100%)
174. Raquel Andrei a Pinheiro Vieira (Licenciatura / Não aplicável (Estudante) / 100%)
175. Raquel Castro Alves Ferreira da Silva (Licenciatura / Não aplicável (Estudante) / 100%)
176. Renata Isabel de Sousa Gonçalves (Licenciatura / Não aplicável (Estudante) / 100%)
177. Ricardo Manuel Campinho Capela (Licenciatura / Não aplicável (Estudante) / 100%)
178. Sofia Raquel Soares Mesquita (Licenciatura / Não aplicável (Estudante) / 100%)
179. Vítor Damião França Almeida (Licenciatura / Não aplicável (Estudante) / 100%)
180. Jorge Manuel Ferreira de Assis (Licenciatura / Apoio Técnico / 50%)
181. Ana Isabel Carvalho Machado (Licenciatura / Técnico Superior e Técnico / 100%)
182. Ana Margarida Araújo Amaral (Licenciatura / Técnico Superior e Técnico / 100%)
183. Andreia Sofia Pires Pinto (Licenciatura / Técnico Superior e Técnico / 100%)
184. Jimmy Melro James (Licenciatura / Técnico Superior e Técnico / 100%)
185. João Carlos Sendão Silva (Licenciatura / Técnico Superior e Técnico / 25%)
186. João Eugénio Bernardino Pena dos Reis (Licenciatura / Técnico Superior e Técnico / 100%)
187. Luis Manuel Abecassis Palma (Licenciatura / Técnico Superior e Técnico / 100%)
188. Maria Rosalina Ramos Oliveira Grilo (Licenciatura / Técnico Superior e Técnico / 100%)
189. Marta Sofia Mendes Valente Bernardo (Licenciatura / Técnico Superior e Técnico / 100%)
190. Nádia Margarida Rosário Silva (Licenciatura / Técnico Superior e Técnico / 100%)
191. Pedro António Nobre Soares Pinto das Neves (Licenciatura / Técnico Superior e Técnico /
100%)
192. Pedro Miguel Gama Cláudio (Licenciatura / Técnico Superior e Técnico / 100%)
193. Sofia Isabel Franco Cavaco (Licenciatura / Técnico Superior e Técnico / 100%)
194. Tânia Sofia da Silva Guerreiro (Licenciatura / Técnico Superior e Técnico / 100%)
195. Elsa Maria (Bacharelato / Técnico Superior e Técnico / 100%)
196. Ines Garcia Niza (Ensino Secundário / Estagiário de Investigação / 50%)
9
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10
197. Iolanda Esmeralda Ribeiro Machado Leite de Sousa (Ensino Secundário / Estagiário de
Investigação / 25%)
198. Tiago André Pereira Guedes (Ensino Secundário / Estagiário de Investigação / 10%)
199. Ana Isabel Reis dos Santos (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
200. Ana Mafalda Tomás Correia (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
201. Ana Sofia de Sousa Santos Dias (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
202. Carlos Filipe Lopes de Carvalho (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
203. Cátia Daniela Santos Moreira (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
204. Claúdia Sofia Martins da Silva (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
205. Cristiana Andreia Valente de Oliveira (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
206. Hernandez Cardoso Silva (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
207. Inês Tavares Pinto de Sá Pereira (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
208. Joana Maria Soares Pereira (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
209. Joana Rute Calça Xavier (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
210. Marcelo José Marques Correia (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
211. Maria Rita Motta de Sottomayor Azevedo Leme (Ensino Secundário / Não Aplicável
(bolseiro) / 20%)
212. Mário André Barros da Cunha (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
213. Miguel Neves dos Reis (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
214. Paula Alexandra Rodrigues e Araújo Guedes (Ensino Secundário / Não Aplicável (bolseiro) /
20%)
215. Pedro Calado Martins (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
216. Raquel Carvalho de Ferreira Costa e Almeida (Ensino Secundário / Não Aplicável (bolseiro) /
20%)
217. Ricardo Emanuel Gomes Fernandes da Silva (Ensino Secundário / Não Aplicável (bolseiro) /
20%)
218. Sara de Brito Tavares (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
219. Sérgio Domingos Cardoso Rocha (Ensino Secundário / Não Aplicável (bolseiro) / 20%)
220. Carla Ofélia Ferreira da Silva (Ensino Secundário / Não Aplicável (bolseiro) / 50%)
221. Daniela Elisabete Pinto Ferreira da Cunha (Ensino Secundário / Não Aplicável (bolseiro) /
30%)
222. Joana Filipa Madureira Gaifem (Ensino Secundário / Não Aplicável (bolseiro) / 30%)
223. João Henrique de Oliveira da Silva Pereira (Ensino Secundário / Não Aplicável (bolseiro) /
20%)
224. Paulo Jorge Pereira da Silva (Ensino Secundário / Não Aplicável (bolseiro) / 30%)
225. Miguel Ovidio Simão Viegas (Ensino Secundário / Apoio Técnico / 100%)
226. Marcos António Viegas Granja (Ensino Secundário / Técnico Superior e Técnico / 100%)
227. Verónica Cristina Pereira Mascarenhas (Ensino Secundário / Técnico Superior e Técnico /
100%)
228. Filipa Isabel Mourão Costa Queiroz (Ensino Secundário / Assistente Técnico / 100%)
229. Helena Sofia Fernandes Teixeira (Ensino Secundário / Assistente Técnico / 100%)
230. Rosa Maria Faustino Brissas (Ensino Secundário / Assistente Técnico / 30%)
2.3 Summary
Figure 1. Distribution of Member researchers according to academic degree.
Figure 2. Distribution of Member researchers - PhD holders according to
professional category.
11
3. OBJECTIVES AND ACHIEVEMENTS
3.1 Unit description
CIMAR became an Associated Laboratory (CIMAR LA) in 2002 as a partnership between CIIMAR
(University of Porto) and CCMAR (University of Algarve). Strategic planning and joint actions are
always in close cooperation and granted by the CIMAR organization mode. Anyway, due to the
legal nature of the LA, management of diverse administrative tasks must be carried out
independently by each partner institution, with CIIMAR (current presidency CIMAR) providing at
present the link with FCT.
CIMAR LA is lead by a president which heads a directive board, composed by the directors of
CIIMAR and CCMAR and two members of each centre indicated by the directive committees,
who meet 3-4 times per year to decide on strategic issues and when their implementation at
each partner centre. CIMAR LA is scientifically organized in 4 research lines each with two
coordinators. Because, among others of the distance between Faro and Porto, annual meetings,
in which all members participate, facilitate communication and enable evaluation of progress
and strategic planning. An advisory committee composed of 5 scientific personalities
participates in these annual meetings and produce an advisory report.
As part of the strategic plan, CIMAR LA has established formal institutional links with Instituto
Geológico e Mineiro (IGM, now the Marine Geology department of INETI), especially in the areas
of marine geology covering aspects related to climate change. This relationship will continue
and is progressively and more formally integrated as we develop a common strategy and
complementarily of human resources for investigating the marine environment. More recently,
and as result of an already significant level of collaborative work in research projects and post
graduate student supervision in the areas of behavioural endocrinology, evolutionary biology
and Conservation CIMAR LA has established formal links with the Research Unit on Eco-Ethology
(UIEE) at the Higher Institute of Applied Ethology (ISPA) and an application to FCT has been
made for UIEE to become part of CIMAR.
3.2 General objectives
The main objectives of CIMAR LA are “to understand natural processes in the ocean and the
coastal zone, to study and implement sustainable exploitation of aquatic resources and the
impact of human activities in the environment”. With these objectives from the initial 10 lines of
research, four lines have consolidated: 1) Biodiversity and Management of Aquatic Resources; 2)
Environmental Chemistry and Toxicology; 3) Biology and Marine Biotechnology; 4) Aquaculture.
With the recent recruitment of researchers in the area of physical oceanography one further
line is being considered to be added.
CIMAR LA is a Centre that covers distinct biogeographical areas in Portugal and therefore one of
the objectives is to monitor, compare and contrast the coastal environments in the two regions,
both in the context of nature conservation and impact of human activities, including
aquaculture. At a more fundamental level, marine organisms are a source of models to answer
fundamental biological questions and as a source of molecules for many applications.
12
One of the strategic objectives of CIMAR LA is to extend scientific cooperation and training to
tropical and subtropical regions, especially Portuguese speaking countries.
Parallel and complementary objectives include: a) to intensify links with companies and
whenever possible promote innovation and entrepreneurship among its members, b) promote
outreach activities through interaction with schools, “Ciência Viva” centres and to the wider
public; c) promote networking activities among the scientific communities and various
stakeholders, d) promote training through various activities, including post-graduate courses
and summers schools.
3.3 Main achievements during the year of 2008
The scientific output and competitiveness of CIMAR LA continued to grow as smaller groups
coalesce and the number of research scientists hired is much higher than those that leave. This
is reflected also in the capacity to obtain funds at national and European level. This is
highlighted by approval of infrastructure proposals such as the FP7 ASSEMBLE
(www.assemblemarine.org) and the ESFRI European Marine Biology Resource centre
(www.embrc.eu).
Specific new contributions to the understanding and management of biodiversity, including
several new records and identification of population structure and barriers to gene flow, and
also of genetic imprints of Pleistocene glacial periods on fish, algae and seagrass species.
Significant new contributions were done to improve dietary formulations and feeding practices
for species which are at an early stage of aquaculture domestication.
Significant new contributions to understand mineralization processes in fish and shellfish, with
potential applications in skeletal malformations in aquaculture and to evaluate the effects of
ocean acidification.
Major advances were made when continuing the several ongoing monitoring projects in several
rivers and Portuguese coastal areas: these encompass changes in biodiversity, population
abundance, pathogens and toxicological biomarkers. This activity is key to build historical series.
Experimental approaches complemented such field advances, providing in several cases quite
significant advances in understanding toxicity mechanisms, whereas establishing baseline
knowledge for better interpreting field data.
On a more fundamental science basis, and in addition to relevant structural and functional
insights published in marine biology, new important contributions supported a better
understanding of protein folding and stability, which are key to functionality.
Also relevant was the installation of new equipment and development of new technologies,
contributing to capacity building in several scientific areas. Included in such effort was the
acquisition and setup of a high performance computer, a key aspect towards the development
of oceanographic numerical modelling.
13
Organization, implementation and support of PhD and Masters programmes: PhD Marine and
Environmental Sciences (a major inter university course, with the participation on two LAs,
CIMAR and CESAM), MSc Erasmus Mundus in Marine Biodiversity and Conservation; MSc
Aquaculture and Fisheries; MSc Marine Sciences and Resources; Msc. Environmental
Contamination and Toxicology.
Continuing contribution to science dissemination in schools and to the wider public. Several
hundred students from different levels and backgrounds have benefited.
14
4. ACTIVITIES
4.1 Integrative/multidisciplinary activities during the year of 2008
CIMAR started the implementation of a long-term environmental research programme in the
costal zone aiming at obtaining data for the evaluation of climate changes. For this, some ongoing multidisciplinary projects (e.g. BIOMARES, RAIA) were used for supporting new smaller
projects, some of them financed by internal funding. A national network on Costal Marine
Biodiversity (DivMar, jointly coordinated by CIMAR, CESAM and ISPA), started in 2008 focusing
their activities in four areas of the continental portuguese coast: North of Portugal, Ria de
Aveiro, Parque Natural da Arrábida, and Ria Formosa. A joint laboratory was also created by
researchers of CIIMAR and CCMAR in Viana do Castelo. In 2008, two new groups, located in
Madeira and Açores Archipelago, integrated CIIMAR and will also joint this network in the near
future.
The network activities will be supported by the joint Doctoral School in Marine and
Environmental Sciences, the Erasmus Mundus MSc Science in Marine Biodiversity and
Conservation, and the project “Managing the effects of global climate on estuarine biodiversity
and productivity” (EEA grants).
In 2008, CIMAR integrated a proposal for the creation of the R&D&I consortium in Ocean
Science and Technology - Consortium OCEANOS, linking five state laboratories (INRB/IPIMAR, IH,
IM, LNEC and LNEG), four universities and one research network (IMAR). The proposal has been
accepted by the Minister of Science, Technology and Higher Education in 2009, with a funding
envelop for 10 years. The science plan includes five thematic areas: Ocean Dynamics and
Climate, Seafloor Dynamics, Marine Biogeochemistry, Ecosystem Function and Dynamics, and
Marine Resource Management and Spatial Planning. In addition to the main thematic areas,
three transversal themes are considered: Operational Oceanography, Ocean Data Management,
and Marine Technologies and Infrastructures.
The facilities at CCMAR in Faro were integrated in the European Infrastructure project
ASSEMBLE-Association
of
European
Marine
Biological
Laboratories
(FP7,
www.assemblemarine.org).
As a member of the Institute for the Development of the Knowledge and the Economy of the
Sea (IDCEM), CIIMAR integrated a marine and maritime cluster - OCEANO XXI. One of the
projects that will be financed is the building of the Park of Science and Technology of the
University of Porto, where the new facilities of CIIMAR will be located. During 2008, a significant
effort was made in the planning of the new building that will allow implementing
interdisciplinary actions with other groups of the University of Porto, namely in the area of
engineering.
4.2 Outreach activities during the year of 2008
CIMAR has become increasingly committed to outreach activities to foster the dialogue between
scientists and society, to improve the public knowledge and perception of science and to
increase the interest of the young students towards scientific careers.
15
CIMAR has been scientifically managing two Environmental Monitoring and Interpretation
Centres (CMIAs, ca. 15000 visitors/year) through protocols with the municipalities of
Matosinhos and Vila do Conde (www.cmia-viladoconde.net). The activities promoted during
2008 included the organization of several temporary exhibitions, conferences, courses and
regular in house and field activities for public of different ages. Moreover, two public aquariums
and fisheries museums have been managed by CIMAR researchers: Aguda Littoral Station - ELA
(www.fundacao-ela.pt, ca 50000 visitors/year) and River Minho AQUAMuseum
(www.cimsoft.pt/aquamuseu, ca 30000 visitors/year). Both stations have a special relevance in
what concerns Environmental Education Programs for all age.
CIMAR has participated at the International Polar Year Education & Outreach Programme,
through the LATITUDE60! (http://latitude60.blogspot.com) educational project, that has been
considered as one of the most active and comprehensive projects internationally. Since 2007,
more than 40 activities dealing with education and outreach were developed to explain polar
research, involving more than 200 schools, 400 teachers and thousands of students. The top
events included an educational expedition of 7 high school students integrated in “Students on
ice” to Antarctica and the Polar Science Weekend at Pavilhão do Conhecimento (6500 visitors).
CIMAR regularly participates in the Ciência Viva Programme (http://www.cienciaviva.pt), mostly
through “Scientists go to school” and “Science on Holidays for Young People” actions for
promotion scientific and technological culture among the Portuguese population and more
specifically to encourage the use of experimental methods in science learning. In 2008, the
centre presented a number of scientific themes to elementary and high schools, in collaboration
with the Algarve Ciência Viva Centre, and prepared 22 scientific activities (1-2 weeks each)
aiming to offer high school students the opportunity for first-hand contact with the reality of
scientific research work during their summer vacations.
In 2008, CIMAR started collaboration with the Visionarium - Europarque Science Centre to
implement a national project using Daphnia as biological model for experimental teaching of
sciences, involving 220 high schools, 320 teachers and 2500 students
(http://projectodaphnia0.blogs.sapo.pt/).
CIMAR regularly participates in other events and exhibitions to encourage high school students
to engage in scientific careers, such as the “Junior University” (http://universidadejunior.up.pt/)
and the “Science, Education and Innovation” exhibition organized by the University of Porto.
Moreover, CIMAR maintains its participation in the EXPOMAR exhibition in Algarve, organize
seminars to the general public and encourage the participation of volunteers within the scope of
scientific projects.
CIMAR activities were disseminated through the media, namely in international non-scientific
journals (i.e. CORDIS News, Science for Environmental Policy-DG Environment News Alert
Service), newspapers (i.e. Público, El País, Diário de Notícias), and participation in radio and TV
programs.
16
5. FUNDING
Origin
2004
2005
2006
2007
2008
LA FCT *
1.586.158,00
1.536.008,00
1.092.380,00
2.913.911,00
2.977.162,00
Units FCT **
0,00
0,00
0,00
0,00
0,00
Project FCT
453.275,00
552.605,00
570.952,00
827.775,00
2.020.917,77
Other
National
159.133,00
108.507,00
197.458,00
240.018,00
487.715,32
Other
International
681.716,00
704.412,00
659.879,00
1.082.379,00
1.200.367,88
National
Industry
233.887,00
110.233,00
140.634,00
258.989,00
140.135,50
International
Industry
0,00
0,00
0,00
0,00
0,00
Total
3.114.169,00
3.011.765,00
2.661.303,00
5.323.072,00
6.826.300,47
(*) Base + Programmatic; (**) Base + Programmatic of the units before the criation of the LA.
17
6. GENERAL INDICATORS
6.1 Composition and training
2004
2005
2006
2007
2008
Total
No. of researchers proposed
6
6
5
0
12
29
No. of researchers hired (LA)
0
9
4
2
14
29
Balance
-6
3
-1
2
2
0
No. of Researchers Hired
(Ciência Programme)
0
0
0
0
10
10
154
199
No. of researchers (FTE)
Training Masters (Master
theses completed)
24
25
17
44
57
167
Training PhDs (PhD theses
completed)
8
8
23
37
26
102
18
6.2 Researchers hired
Name
Start date
End date
Carlos Alexandre Sarabando Gravato
02-01-2008
01-11-2011
Cymon John Cox
16-06-2008
15-06-2010
David Manuel Flores Gonçalves
01-05-2008
30-04-2010
Filipe Alexandre Oliveira dos Santos Alberto
01-04-2008
31-03-2011
Isabel Gonçalves de Barbosa Araújo
01-02-2008
31-01-2011
João Carlos dos Reis Cardoso
03-03-2008
28-02-2011
Jorge Manuel dos Santos Goncalves
01-09-2008
01-09-2011
Machiel Simon Bos
01-02-2008
31-01-2011
Ralph Urbatzka
01-04-2008
31-03-2011
Rodrigo da Silva Costa
01-10-2008
30-09-2009
Rui Miguel Andrade Caldeira
01-02-2008
31-01-2011
Susana Maria Pereira da Costa Moreira
01-05-2008
30-04-2011
Sven Wuertz
01-11-2008
31-10-2011
Vicent Laizé
01-10-2008
30-09-2011
Name
Start date
End date
Ana Isabel Carvalho Machado
01-05-2008
Marta Sofia Mendes Valente Bernardo
01-10-2008
6.3 Technical personal hired under LA contract
30-09-2011
19
7. RESEARCH LINES (THEMATIC AREAS)
RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems
7a. General description
1. Designation
Ecology, Biodiversity and Management of Aquatic Ecosystems
2. Principal investigator
Karim Erzini
3. Main scientific domain
Marine Sciences
7b. Objectives and achievements
1. General objectives
The general objectives of RL1 are to study selected aspects of marine geology, oceanography,
marine biodiversity and ecosystems, integrating research to provide a sound scientific basis for
assessment, management, and policy related with the marine ecosystems. Within this
framework, the objectives include the following:
-
Promote the geological research of the oceans and coastal regions, the
paleoceanography, and the processes related to the occurrence of marine mineral
resources;
-
Determine the composition, abundance and distribution of the national marine
biodiversity;
-
Determine the impacts of climate change and local human action by monitoring and
studying coastal and estuarine ecosystem functioning, and long-term changes in marine
communities and habitats;
-
Study the genetics, population biology, ecology and recruitment of ecologically and
commercially important species;
-
Investigate the physiological adaptations of migratory fishes, to different salinity and
hydrostatic pressure;
-
Participate in International networks on marine biodiversity;
20
-
Train young scientists and to disseminate scientific knowledge.
2. Main achievements
-
Significant advances in biogeography and marine reproductive ecology, including
identification of population structure and barriers to gene flow and genetic imprints of
Pleistocene glacial periods on fish, algae and seagrass species, and the development of
novel molecular and statistical methods for clonal population genetics and species in
endangered habitats.
-
Important contributions to the knowledge of the marine biodiversity and its distribution
and abundance, and including many new records.
-
Development of ecological and ecohydrologic models that allow the evaluation of
human impacts on ecosystems, in particular estuarine and coastal.
-
Scientific contributions for the assessment and management of human impacts on
protected coastal systems,and for management of commercially exploited species.
-
Monitoring of the Arade, Guadiana,Minho, Lima and Douro estuaries, the Ria Formosa
lagoon, and the Litoral Norte site.
-
First comprehensive evaluation of the carbon budget of the intertidal area of Ria
Formosa coastal lagoon concluded.
-
Significant improvements in the production of cuttlefish and sea horses achieved
-
Identification of ammocoete metamorphosis as the critical time in the acquisition of
marine ionoregulatory competence in the anadromous marine lamprey.
-
3 new ecotoxicity lab bioassays linking behaviour with biomarkers (marine, soil).
-
Validation of 6 native species (crustaceans, bivalves, fish, reptiles, birds) biomarkers for
use in biomonitoring studies in temperate and tropical ecosystem.
-
Acquisition and setup of a High Performance Computer, a key aspect towards the
development of oceanographic numerical modelling.
-
Use of Mobile Mapping Technologies to build Digital Terrain Models (DTM) for coastal
erosion studies, in particular the characterization of the morphodynamic evolution of
sandy littorals.
-
Participation in EU networks.
-
Participation in Erasmus Mundus MSc in Marine Biodiversity and Conservation.
21
7c. Research lines output
1. Collaborative publications in peer review journals (If more that one Research Group of
the LA is involved. Title and full citation in original language)
Examples of collaborative publications:
1. Baptista, MS, Vasconcelos, MTSD, Cabral, JP, Freitas, MC, Pacheco, AMG. 2008. Copper, nickel
and lead in lichen and tree bark transplants over different periods of time. Environmental
Pollution 151: 408-413.
2. Batista, FM, et al. 2008. Comparative study of shell shape and muscle scar pigmentation in the
closely related cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids.
Aquatic Living Resources 21: 31-38.
3. Cunha, SR, GonçalveS, R., Silva, SR, Correia, AD. 2008. An automated marine biomonitoring
system for assessing water quality in real-time. Ecotoxicology 17: 558-564.
4. Engelen, A, Espirito-Santo, C, Simões, T, Monteiro, C, Serrão, EA, Pearson, GA, Santos, R. 2008.
Periodicity of propagule expulsion and settlement in the competing native and invasive brown
seaweeds, Cystoseira humilis and Sargassum muticum (Phaeophyta). European Journal of
Phycology 43: 275-282. IF=1.826.
5. Fonseca, PJ, Serrão, EA, Pina-Martins, F, Silva, P, Mira, S, Quartau, JA, Paulo, OS, Cancela, ML.
2008. The evolution of cicada songs contrasted with the relationships inferred from
mitochondrial DNA (Insecta, Hemiptera). Bioacoustics 18: 17-34.
6. Pacheco, AMG, Freitas, MC, Baptista, MS, Vasconcelos, MTSD, Cabral, JP. 2008. Elemental
levels in tree-bark and epiphytic-lichen transplants at a mixed environment in mainland
Portugal, and comparisons with an in situ lichen. Environmental Pollution 151(2): 326-333.
7. Pacheco, AMG, Freitas, MC, Baptista, MS, Vasconcelos, MTSD, Cabral, JP. 2008. Joint
assessment of responses of biomonitors to airborne nickel and vanadium through nuclear and
non-nuclear techniques. Journal of Radioanalytical and Nuclear Chemistry 276(1): 135-141.
8. Sousa, R, Antunes, C, Guilhermino, L. 2008. Ecology of the invasive Asian clam Corbicula
fluminea (Muller, 1774) in aquatic ecosystems: an overview. Annales de LimnologieInternational Journal of Limnology 44: 85-94.
9. Sousa, R, Dias, S, Guilhermino, L, Antunes, C. 2008. Minho River tidal freshwater wetlands:
threats to faunal biodiversity Aquatic Biology 3: 237-250.
10. Sousa, R, Morais, P, Antunes, C, Guilhermino, L. 2008. Factors affecting Pisidium amnicum
(Müller, 1774) (Bivalvia: Sphaeriidae) distribution in the River Minho estuary: consequences for
its conservation. Estuaries and Coasts 31: 1198-1107.
11. Sousa, R, Nogueira, AJA, Antunes, C, Guilhermino, L. 2008. Growth and production of
Pisidium amnicum (Müller, 1774) in the freshwater tidal area of the River Minho estuary.
Estuarine, Coastal and Shelf Science 79: 467-474.
22
12. Sousa, R, Nogueira, AJA, Gaspar, M, Antunes, C, Guilhermino, L. 2008. Growth and extremely
high production of the non-indigenous invasive species Corbicula fluminea: possible implications
for ecosystem functioning. Estuarine Coastal and Shelf Science 80: 289-295.
13. Sousa, R, Rufino, M, Gaspar, M, Antunes, C, Guilhermino, L. 2008. Abiotic impacts on spatial
and temporal distribution of Corbicula fluminea (Muller, 1774) in the River Minho Estuary,
Portugal. Aquatic Conservation-Marine and Freshwater Ecosystems 18: 98-110.
2. Collaborative other publications (If more than one group is involved. Books, chapters or
full papers published in conference proceedings. Title and full citation in original
language)
3. Master and PhD theses completed (Co-supervision or clearly multidisciplinary projects)
Examples of collaborative Master and PhD theses:
MASTER THESES
Ferreira, C. 2008. Molecular and sub-cellular approaches in soil ecotoxicology. Master thesis in
Molecular and Cell Biology, University of Aveiro. Supervisors: AMVM Soares (University of
Aveiro, CESAM), C Gravato (CIMAR-ECOTOX), M Amorim (CESAM).
Gonçalves, O. 2008. Gastric proton pump expression in teleost fishes: nongastric sites of
expression in gastric versus agastric fishes. Master thesis, University of Tras-os Montes e Alta
Douro. Supervisors: Jonathan Wilson (CIMAR-Ecophysiology), A Fontaínhas Fernandes (UTAD).
Martins, MAS. 2008. Effects of pollutants and natural stressors on populations of planktonic
organisms. Master thesis in Biology, University of Aveiro. Supervisors: L Guilhermino (CIMAREcotoxicology), AMVM Soares (University of Aveiro, CESAM).
Rodrigues, J. 2008. A avifauna do estuário do Rio Minho (aves aquáticas). Caracterização,
importância e perspectivas de uso turístico. Master thesis in Sciences of the Sea - Sea Resources,
ICBAS, University of Porto. Supervisors: António da Silva Luís (University of Aveiro), AMF Bio
(CIMAR-OCD).
PhD THESES
Antunes, PJ. 2008. Accumulation of PCB congeners and DDT compounds in fish: influence of size
and reproductive cycle. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. In
collaboration with INIAP/IPIMAR. Supervisors: Odete Gil (INIAP/IPIMAR), MA Reis-Henriques
(CIMAR-Environmental Toxicology).
Azevedo, MI. 2008. Ecological modelling of Douro Estuary: influence of river flow variability on
estuarine water quality and primary production. PhD in Aquatic Sciences, ICBAS, Univ. Porto.
Supervisors: AA Bordalo (CIMAR-Hydrobiology), P Duarte (Univ. Fernando Pessoa).
23
Rocha, AM. 2008. Clonação, caracterização funcional e expressão de receptores das
gonadotrofinas (FSH e LH) no robalo europeu (Dicentrarchus labrax, L.). PhD thesis in Aquatic
Sciences, ICBAS, University of Porto. In collaboration with CSIC, Univ. Sevilha. Co-supervisor: J
Coimbra (CIMAR-Ecophysiology).
Silva, C. 2008. Reproductive Ecology of the mildly sex-role reversed pipefish, Syngnathus abaster
(Pisces; Syngnathidae). PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor:
MN Vieira (CIMAR-Biodiversity of Aquatic Ecosystems), Vitor Almada (ISPA).
Sousa, R. 2008. Factors contributing to the invasive success of Corbicula fluminea (Müller, 1774).
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisors: L Guilhermino (CIMAREcotoxicology), C Antunes (CIMAR- Ecophysiology).
Stronowski, Z. 2008. A High - Resolution climate study on the Norwegian & Iberian Shelves
during the Late Holocene: a diatom perspective. In collaboration with Univ. Plymouth.
Supervisor: F Abrantes (CIMAR-Geology and Paleoceanography).
7d. Future research
1. Other information (If more that one group is involved. Patents/prototypes, organization
of conferences, industry contract research)
Members of the RL1 were involved in the creation of patents, organization of both National and
International conferences, and industry contract research.
Patents
-
Portable equipment for measuring and calculating locomotion dynamic parameters,
National patent request no. 103933, Jan 2008. M Correia, S Cunha.
-
2 devices for measuring swimming velocity and water flow resistance in fish bioassays 1
device for measuring avoidance in shrimps.
Conferences organization
-
Bayesian Evolutionary Analysis Sampling Trees – BEAST, 29-30 May 2008. Lecturers:
Philippe Lemey (Leuven University, Belgium) and Beth Shapiro (Pennsylvania State
University, USA).
-
III Encontro Nacional de Estudantes de Pós-Graduação em Ciências Biológicas. Faro, 3-5
September 2008.
-
8th GAP International Workshop on Gross and Net Primary Production: Closing the Gap
between Concepts and Measurements. 30 March-8 April 2008, Coordination of the
seagrass research group, Eilat, Israel.
24
-
International workshop on Telemetry- theory, concepts and applications, Faro, 29
September 2008.
-
International workshop on Acoustic telemetry- Equipments and demonstrations, Faro,
30 September 2008.
-
International Congress Seasink 2008. University Fernando Pessoa, 26-28 July 2008.
-
International Workshop on Dynamics of island wakes, Funchal, 21-31 July 2008.
-
7th Iberian and 3rd Iberoamerican Congress of Environmental Contamination and
Toxicology – CICTA 2008, Lisbon, 10-12 March 2008. (Scientific Committee)
-
18th Annual Meeting of SETAC-Europe, Warsaw, 25-29 May (Scientific Committee)
For 2009:
-
6th European Conference on Marine Natural Products, Porto, 19-23 July 2009.
-
BIOLIEF, World Conference on Biological and Ecosystem Functioning, Porto, 27-30
October 2009.
-
2nd European Congress of Conservation Biologists, Prague, September 2009. (Scientific
Committee)
Industry contract research
-
Evaluation of algal removal impact in the area adjacent to the thermoelectric power
plant in Sines bay. Research contract with Hidromod/EDP-Electricidade de Portugal,
2008.
-
BIOMARES LIFE project. Partly funded (50%) by SECIL. Partnership includes members of
CIMAR-FBC and CIMAR-BEE, IPIMAR, ICNB, NOAA (USA) and CSIC (Spain), 2007-2010.
-
Estudo de impacte ambiental da dragagem de inertes na costa continental portuguesa –
Componente biologica Algarve. Research contract with Amb & Veritas, 2007-2008.
-
Estudo de reabilitação e valorização do troço de costa entre praia D. Ana e a Praia do
Canavial (Lagos)-Ambiente Marinho. Research contract with Câmara Municipal de Lagos,
HB Arquitectura, CCDR Algarve, 2008.
For 2009:
-
Conservação e análise do registo histórico dos marégrafos de Leixões. Research contract
with Port Authority of Douro and Leixões (APDL).
-
Plano nacional de conservação da lampreia-de-rio e da lampreia-de-riacho. Contract
research with EDP, Biodiversity fund.
25
2. Future plans
Continue integration of the groups, increasing the synergies between present and the new
researchers that are being hired, thereby reinforcing existing lines of research, and developing
new research lines that will greatly improve our collective research capacity and the response
to societal needs. So within the continuation of the general objectives of the research line we
will:
-
Increase our research on phylogeography, edge population genetics, genetics of
metapopulation systems, coastal and deep sea connectivity, invasive species, and
genome-wide patterns of stress responsive gene expression;
-
Contribute to global databases (e.g. Algabase, GBIF, OBIS), international research
programs (CoML and its successor 2020) and national initiatives such as the network
for Marine and Coastal Biodiversity;
-
Consolidate international cooperation, including participation in innovative educational
programs at the European level (e.g. Erasmus Mundus Masters in Marine Biodiversity
and Conservation);
-
Use otolith finger printing as biological tracers of fish migration and for stock:
discrimination, and of transcriptomics to study physiological adaptation in fishes
-
Enhance research on fish larval trophodynamics,production, population dynamics and
ecology, spatio-temporal dynamics and essential fish habitats;
-
Monitor coastal erosion, biodiversity and habitats to study the effects of global
environmental changes and local anthropogenic effects, including the impacts of alien
species on coastal ecosystems, and effects of marine acidification;
-
Contribute to the implementation of environmental and marine policies e.g. the EU
Water Framework Directive (EEMA project), the Habitats Directive for the marine
environment, and other EU Directives, such as the Marine Strategy Directive and ICZM
(e.g. POEM);
-
Develop and implement hydrodynamic and biogeochemical models, and decision
support systems (DSS), to integrate knowledge about coastal ecosystems and to be
used as management tools;
-
Continue to work on restoration of biodiversity and habitats and evaluation of the
benefits of a marine protected areas;
-
Assess toxicity effects of wastewaters using different test organisms and develop and
apply eco-technology techniques for improvement of water quality.
26
RL2 - Environmental Chemistry and Toxicology
1. Designation
Environmental Chemistry and Toxicology
Maria Teresa Sa Dias de Vasconcelos
Environment
Foster cooperation and pursue high quality research, whereas developing innovative solutions
and applications, in three areas:
-
Chemical speciation of xenobiotics and natural species to get answers for the following
questions: how do chemicals of a medium influence the response of an organism in
contact with it? how do organisms affect the chemical composition of the surrounding
medium?
-
Evolution and functioning of ecosystems under anthropogenic and natural stress to
better understand the effects of pollutants (e.g. endocrine disrupting compounds,
pharmaceutics, persistent organic and organo-metallic pollutants, PAHs, heavy metals)
on aquatic organisms and ecosystems. Use of integrated strategies with methods and
tools from ecotoxicology, ecology, genetics, chemistry; development of novel holistic
approaches, namely based on the use of batteries of sensitive biological tests for
diagnosis, monitoring, modelling and remediation of pollution.
-
Ecotoxicology of cyanobacteria and other potentially toxic organisms in marine, brackish
and freshwaters; characterization of toxins, using molecular and computational
genomics /proteomics.
Technological
-
Validation of 7 biomarkers from diversified native species to use in biomonitoring
studies in temperate and tropical ecosystems.
-
Identification of antagonistic effects of TBT and ECDS in gastropods.
27
-
Development of multiparametric monitoring systems for assessment of pollution and its
effects on estuarine and marine ecosystems.
-
Implementation of new methods for speciation of toxicants or biologically produced in
response to toxicity.
-
Optimization of some biological remediation solutions for confined areas.
Scientific
Relevant work was carried out on:
-
Identification of mechanisms of action of several environmental contaminants on
aquatic organisms and those involved in genetic resistance to chemical contamination,
including molecular mechanisms underlying imposex in gastropods, xenoestrogens in
vitellogenin induction in fish, biochemical and populational mechanisms of genetic
resistance in cladocerans and marine copepods, mechanisms of cholinesterase ChE
inhibition by metals, among others.
-
Portuguese marine cyanobacteria that produce apoptotic activity in neuroblastoma cells
and that are good candidates for pharmaceutical research. The use of ELISA and of
multiplex PCR proved that many dietary supplements are contaminated with the
hepatotoxic microcystin. Genomic studies across organisms contributed to understand
the functional changes of various gene/proteins involved in the metabolism of drugs
and toxins
-
Contamination levels in NW Portuguese coast in Minho, Lima, Cávado and Douro Rivers
estuaries and Aveiro lagoon, using integrated approaches including water quality
variables, chemical analysis (metals, PAHs, pesticides, TBT), natural toxins,
bioaccumulation factors, biomarkers and condition indexes.
Support for advanced training
-
Know-how and technical facilities have been used to support a large number of
undergraduate, MSc and PhD students during the preparation of their theses.
-
International recognition by publishing houses and editors:
-
Several members of RL2 are members of Editorial Board of International Journals, and
one of them is Editor-in-chief. Regular reviewers of manuscripts submitted to ISI
journals.
28
1. Collaborative publications in peer review journals (If more that one Research Group of
1. Almeida, CMR, Mucha, AP, Bordalo, AA, Vasconcelos, MTSD. 2008. Influence of a salt marsh
plant (Halimione portulacoides) on the concentrations and potential mobility of metals in
sediments. Science of the Total Environment 403: 188-195
2. Almeida, CMR, Mucha, AP, Delgado, MFC, Caçador, IM, Bordalo, AA, Vasconcelos, MTSD.
2008. Can PAHs influence Cu accumulation by salt marsh plants? Marine Environmental
Research 66: 311-318.
Pollution 151: 408-413
4. Fernandes, C, Fontaínhas-Fernandes, A, Rocha, E, Salgado, MA. 2008. Monitoring pollution in
Esmoriz-Paramos lagoon, Portugal: Liver histological and biochemical effects in Liza saliens.
Environmental Monitoring and Assessment 145: 315-322.
5. Morais, J, et al. 2008. European Food Research & Technology 227: 949-952.7.
6. Mucha, AP, Almeida, CMR, Bordalo, AA, Vasconcelos, MTSD. 2008. Salt marsh plants (Juncus
maritimus and Scirpus maritimus) as sources of strong complexing ligands. Estuarine, Coastal
and Shelf Science 77(1): 104-112.
9. Reis, PA, et al. 2008. A fast and simple methodology for determination of yttrium as an inert
marker in digestibility studies. Food Chemistry 108(3): 1094-1098.
10. Santos, MM, et al. 2008. Anti-androgenic effects of sewage treatment plant effluents in the
prosobranch gastropod Nucella lapillus. Comparative Biochemistry and Physiology, Part C 148
(1): 87-93.
11. Sousa, R, Dias, S, Freitas, V, Antunes, C. 2008. Subtidal macrozoobenthic assemblages along
the River Minho estuarine gradient (north-west Iberian Peninsula). Aquatic Conservation –
Marine and Freshwater Ecosystems 18: 1063-1077.
fluminea (Muller, 1774) in aquatic ecosystems: an overview. Annales de LimnologieInternational Journal of Limnology 44: 85-94.
29
threats to faunal biodiversity. Aquatic Biology 3: 237-250.
its conservation. Estuaries and Coasts 31: 1198-1107.
Estuarine, Coastal and Shelf Science 79: 467-474.
Marine and Freshwater Ecosystems 18: 1063-1077.
17. Tiago, DM, Laizé, V, Cancela, ML, Aureliano, M. 2008. Impairment of mineralization by
metavanadate and decavanadate solutions in a fish bone-derived cell line. Cell Biology and
Toxicology 24: 253-263. IF=2.155.
18. Tiago, DM, Laizé, V, Aureliano, M, Cancela, ML. 2008. Vanadate proliferative and anti
mineralogenic effects are mediated by MAPK and PI3K/Ras/Erk pathways in a fish chondrocyte
cell line. FEBS Letters 582: 1381-1385.
language)
Collaborative other publications:
1. Tiago, DM, Laizé, V, Aureliano, M, Cancela, ML. 2008. Vanadate effects on bone metabolism:
fish cell lines as an alternative to mammalian in vitro systems In: MA Alves (ed). Vanadium
Biochemistry. Research Signpost, India, pp. 269-283.
2. Tiago, T, Aureliano, M, Gutiérrez-Merino, C. 2008. Effects of reactive oxygen and nitrogen
species on actomyosin and their implications for muscle contractility. In: Oxidative stress,
Research Signpost, Kerala, India. (in press).
MASTER THESES
Molecular and Cell Biology, University of Aveiro. Supervisor: AMVM Soares (CESAM, Univ.
Aveiro); Co-supervisors: C Gravato (CIMAR-ECOTOX), M Amorim (CESAM).
30
organisms. Master thesis in Biology, University of Aveiro. Supervisor: L Guilhermino (CIMARECOTOX), Co-supervisor: AMVM Soares (Univ. Aveiro, CESAM).
Martins, J. 2008. Estudo das comunidades de cianobactérias de uma ETAR (Águas de Gaia).
Avaliação molecular e toxicológica e efeitos nas comunidades bacterianas. Master thesis in
Quality Control, Faculty of Pharmacy, University of Porto. Supervisor: V Vasconcelos (CIMARLEGE), Co-supervisor: Luísa Peixe (FFUP).
Andrade, M. 2008. Avaliação dos efeitos morfológicos e ultraestruturais de microcistinas em
células Vero. Master thesis in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor:
V Vasconcelos (CIMAR-LEGE), Co-supervisor: Paulo Pereira (INSA-Lisbon).
PhD THESES
and reproductive cycle. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor:
Odete Gil (INIAP/IPIMAR), Co-supervisor: MA Reis-Henriques (CIMAR-Environmental
Toxicology).
estuarine water quality and primary production. PhD thesis in Aquatic Sciences, ICBAS,
University of Porto. Supervisor AA Bordalo (CIMAR-Hydrobiology), Co-supervisor: Pedro Duarte
(University Fernando Pessoa).
Pinto, CB. 2008. Os peroxissomas da truta fário (Salmo trutta fario): regulação por compostos
estrogénicos. Supervisors: A Lobo da Cunha and E Rocha (CIMAR-LECEM), P Rodrigues
(IBMC/INEB LA, ICBAS).
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Guilhermino (CIMARECOTOX), Co-supervisor: C Antunes (CIMAR-Ecophysiology).
Tiago, DM. 2008. 2008. Role of insulin and insulin like peptides in bone formation: identification
of bone specific target genes and regulatory mechanisms, and characterization of the insulin
mimetic effect of vanadium. PhD thesis, University of Algarve. Co-supervisor: ML Cancela and V
Laizé (CIMAR-LEGE), M Aureliano (CIMAR-BioVanadium).
7d. Future research
As to industry contract research, the aim is to continue to provide advanced services to industry,
not only via the current established contracts (e.g. detection of natural toxins in consuming
water – see more information in the details provided by the individual groups) but also
promoting new ones.
31
As to the Organization of Conferences, in the area of RL2 we can state that the pole holds all the
know-how, networking, and other bases to attract and house internationally established
meetings and even launching new important series of conferences. Two conferences organized
by CIMAR are programmed for 2009:
-
1st Iberian Conference on Cyanotoxins. FCUP, 6-7 July 2009, Porto, Portugal.
-
BIOLIEF, World Conference on Biological Invasions and Ecosystem Functioning, 27-30
October 2009, Porto, Portugal.
2. Future plans
Research targets
-
Current/pending projects assure funding to sustain high quality research.
-
Integrating methods at different levels of biological organization, i.e. from changes in
gene expression up to alterations on population dynamics and ecosystem functioning.
The effects of chemical pollution and natural stressors on the cited functioning will be
investigated using both in situ and laboratory experiments. We aim to implement risk
assessment, namely targeting both natural toxins and man made xenobiotics (e.g. oil
and chemical spills, PAH, pharmaceuticals), using multidisciplinary approaches in
marine, brackish water and freshwater environments. Risk assessment will also
integrate the potential impact of global changes on the effects of pollution in temperate
and tropical ecosystems.
-
The variation of the levels of contamination will continue to be quantified with the
collaboration of groups from other research lines, articulating such information with the
development of early warning methods for the detection and quantification of
cyanotoxins and of chemical contaminants, using molecular and ecotoxicological
approaches.
-
Efforts at cell level will continue, targeting effects and mechanisms. The effects of
vanadium compounds with insulin mimetic properties in calcium regulation and also the
mechanisms of cell death induce by vanadate oligomers will be studied. In the field of
endocrine disruption we will continue the research on chemical mixtures with additive
and antagonistic effects.
-
Evolutionary genomics/proteomics studies of protein-coding genes operating in
detoxification, development, immune system and genetic disease onset will expand
previous approaches. We aim to better clarify the roles of phases I, II and III of
detoxification, namely the transport proteins involved in the elimination of xenobiotics
and natural toxins.
Networking
-
32
Continue to participate in networks, cooperating with organizations from different
countries through change of students/researchers and projects; participate in the
organization of national and international conferences. Cooperation with national and
local authorities e.g. the National Environmental Agency, Water Institute and Health
Authorities will support policy developments and hazard assessment of chemicals.
33
RL3 - Biology and Marine Biotechnologies
1. Designation
Biology and Marine Biotechnologies
Deborah Mary Power
Biological Sciences
The groups contributing to this section and their overall objectives have been maintained since
the 2003-2007 report. Namely, to extend fundamental knowledge about marine organisms at a
molecular to whole organism level and across the evolutionary scale. Establish how natural and
man made environmental challenges impact organismal function. Reinforce the drive to
promote the identification of potential “spin-offs” arising from basic biology and stimulate pilot
studies and links with industry (eg. “near market” solutions, such as, genotyping for aquaculture
species, diagnostic tests in pathology). Ensure the acquired knowledge is disseminated in
learned journals of good impact and at International conferences and by the establishment of
collaborative networks. Actively encourage and educate stake holders (scientists, policy makers,
industry, teachers and the public) about the diversity, complexity and importance of the marine
biotope.
Herein only major achievements, those perceived as having a significant impact at a national or
international level are indicated (see individual group reports for specific details):
-
Identification of the mechanisms and critical time in the acquisition of marine
ionoregulatory competence; of relevance to understanding organismal evolution and
adaptation, practical implications in aquaculture and conservation biology (eg.
anadromous marine lamprey (Petromyzon marinus).
-
Scientific and technical contribution to understanding skeleton and shell development in
fish and shellfish, respectively, and factors contributing to development of
malformations in aquaculture.
34
-
Characterization and monitoring of pathogens of Marine vertebrates (fish) and
invertebrates (clams) and biomarkers developed for toxicological monitoring of marine
invertebrates and vertebrates.
-
Further development and refinement of in silico protein modeling methods and data
about the impact of vibrational excited states (VES) of amide I on the Davydov/Scott
model which are contributing to understanding protein folding and stability.
-
Establishment of a range of new technologies and capacities by groups in the research
line to enhance R&D capabilities. Including establishment of high-throughput techniques
such as Fluorescent-Activated Cell Sorting (FACS) adapted to microalgae.
-
Establishment of stable transgenic cell lines and fish cell lines.
-
Initiation of a number of new research lines in the RL groups, for example, use of algae
for biofuels, screening of chemicals and natural compounds using electrolfactograms,
reverse pharmacology methods, microRNAs.
-
Training of PhD and MSc and visiting scientists and technology transfer activities
including specialist workshops and training and technology transfer to developing
countries.
-
Stimulation of debate through interaction with schools through open lectures, open
days, short-study visits by students (Ciência Viva) and resource development.
1. Collaborative publications in peer review journals (If more that one Research Groups of
1. Coesel, SN, Teles, LM, Baumgartner, AC, Henriques, NM, Cancela, L, Varela, JC. 2008. Nutrient
limitation is the main regulatory factor for massive β-carotene accumulation and for Psy and Pds
transcript levels in Dunaliella salina exposed to light and salt stress. Marine Biotechnology 10:
602-611.
mitochondrial DNA (Insecta, Hemiptera). Bioacoustics 18: 17-34.
3. Ramos, A, Marques, A, Rodrigues, M, Henriques, N, Baumgartner, A, Castilho, R, Brenig, B,
Varela, J. 2009. Molecular and functional characterization of a cDNA encoding 4-hydroxy-3methylbut-2-enyl diphosphate reductase from Dunaliella salina. Journal of Plant Physiology 166:
968-977.
35
4. Martins, RF, Ramos, MF, Herfindal, L, Sousa, JA, Skaerven, K, Vasconcelos, V. 2008.
Antimicrobial and cytotoxic assessment of marine cyanobacteria - Synechocystis and
Synechococcus. Marine Drugs 6: 1-11.
5. Santos, MM, Reis-Henriques, MA, Guillot, R, Lima, D, Franco-Duarte, R, Mendes, I, Queirós, S,
Castro, LF. 2008. Anti-androgenic effects of sewage treatment plant effluents in the prosobranch
gastropod Nucella lapillus. Comparative Biochemistry and Physiology 148: 87-93.
6. Tanguy, A, et al. 2008. Increasing genomic information in bivalves through new EST collections
in four species: development of new genetic markers for environmental studies and genome
evolution. Gene 408: 27-36.
7. Tiago, DM, Cancela, ML, Aureliano, M, Laizé, V. 2008. Vanadate proliferative and anti
cell line. FEBS Letters 582: 1381-1385.
Toxicology 24: 253-263.
language)
1. Canário, AVM, Scott, AP, Hubbard, PC, Barata, EN (eds). 2008. Bioactive water-borne
chemicals: pheromones and welfare indicators - the Faro Workshop. Special Issue Behaviour
Volume 145, Number 10.
2. Oliveira, RF, Canario, AVM, Ros, AFH. 2008. Hormones and alternative reproductive tactics in
vertebrates. In: Oliveira, RF, Taborsky, M, Brockmann, HJ. Alternative Reproductive Tactics: an
Integrative Approach. Cambridge University Press, pp. 132-173. ISBN 978-0-521-83243-4.
3. Sundell, K, Power, DM (Eds). 2008. The use of molecular methods and functional genomics in
aquaculture. Special issue Reviews in Fisheries Science, Volume 16, Issue S1.
MASTER THESES
Batista, S. 2008. The effect of the replacement of fishmeal to algae flour on growth and
metabolic parameters in gilthead sea bream (Sparus aurata). Master thesis in Biological
Engineering, University of Algarve.
Bicho, R. 2008. Efeito do cloro no desenvolvimento e no eixo tiroide da Tilapia Moçambicana
(Oreochromis mossambicus). Master thesis in Biotechnology, University of Algarve.
36
Gonçalves, O. 2008. Gastric proton pump expression in teleost fishes: nongastric sites of gastric
versus agastric fishes. Master thesis, University of Tras-os-Montes e Alto Douro (UTAD).
Supervisor: J Wilson (CIMAR-Ecophysiology), Co-supervisor: A Fontaínhas Fernandes (UTAD).
Rosa, I. 2008. Influence of high stocking density, fasting and refeeding on intermediate
metabolism of red porgy Pagrus pagrus. Master thesis in Biological Engineering, University of
Algarve.
PhD THESES
Delgado, C. 2008. Gonad development and hormone titres in loggerhead sea turtles Caretta
caretta in the NE Atlantic. PhD thesis, University of Madeira. Supervisors: A Canário (CIMARCME).
Pinto, CB. 2008. Os peroxissomas da truta fário (Salmo trutta fario): regulação por compostos
estrogénicos. Supervisors: A Lobo da Cunha, E Rocha and P Rodrigues (IBMC / INEB LA, ICBAS).
Rocha, AM. 2008. Clonação, caracterização funcional e expressão de receptores das
gonadotrofinas (FSH e LH) no robalo europeu (Dicentrarchus labrax, L.). PhD thesis in Aquatic
Sciences, ICBAS, University of Porto. In collaboration with CSIC, Univ. Sevilha. Co-supervisor: J
Coimbra (CIMAR-Ecophysiology).
PhD thesis in Aquatic Sciences, ICBAS, Univ. Porto. Supervisor: L Guilhermino (CIMAR-ECOTOX),
Co-supervisor: C Antunes (CIMAR- Ecophysiology).
Tiago, DM. 2008. Role of insulin and insulin like peptides in bone formation: identificatin of bone
specific target genes and regulatory mechanisms, and characterization of the insulin mimetic
effect of vanadium. PhD thesis, University of Algarve. Supervisors: ML Cancela and V Laizé
(CIMAR-EDGE) and M Aureliano (CIMAR-BioVanadium).
7d. Future research
Members of the RL3 were involved in the organization of both National and International
conferences during 2008, only those actions which involved different groups from the RL3 or
other RL in CIMAR or of an interdisciplinary character are indicated.
Conferences
-
16th National Congress of Biochemistry, Ponta Delgada, Azores, 22-25 October 2008.
This event was organized by the Portuguese Biochemical Society (SPB). Leonor Cancela
and João Varela members of the SPB Board of Directors.
37
-
International Congress Seasink 2008. University Fernando Pessoa. Porto, Portugal. 2628 July 2008.
In 2009:
-
7th AIEC Congress, Iberian Association of Comparative Endocrinology, 6-9 September
2009, Porto, Portugal.
Patent application
-
Portable equipment for measuring and calculating locomotion dynamic parameters,
National patent request no. 103933, Jan 2008. M Correia, S Cunha.
Outreach activities:
-
Latitude60! funded by Agência Ciência Viva (350.000 EUR) between March 2007 and
March 2009 was the largest outreach project carried out as yet in Portugal. It was
devoted to the polar regions and planned to coincide with International Polar Year.
More than 170 schools, 300 teachers and thousands of students were involved
(http://tiny.cc/Kaq6a). This action was coordinated by Adelino Canario of RL3 and
counted with the collaboration of various members of CIMAR.
Networking Actions
-
Marine Genomics Europe (MGE), the FP6 network of excellence (www.marinegenomics-europe.org) reached the end of EC funding in 2008. Continuation of MGE will
take different forms: a) Groupement de recherche internationelle (GRI-CNRS); b) joint
training activities; c) infrastructures, etc. ASSEMBLE (www.assemblemarine.org) and the
establishment and implementation of these actions are being coordinated by Adelino
Canario and will benefit all CIMAR members.
2. Future plans
All the research groups involved in the RL3 will aim to consolidate their impact on their existing
areas of expertise and capacities by seeking funding and when appropriate establishing
complimentary collaboration. The research area now contains a number of well consolidated
groups and the time is right to conduct an internal audit to establish common needs and a
“vision” for the future evolution of RL3.
In this context:
-
Steps will be taken to identify complimentary and specific research goals and develop a
cohesive plan to stimulate cooperation between different research groups within the
research line and across research lines with the aim of reducing fragmentation and
stimulating multidisciplinary research;
-
Identification of common technology needs in RL3 and establish a strategy to stimulate
the establishment of relevant “technology platforms”;
38
-
Promote the links with industry through interaction with technology transfer centers
(eg. CRIA, Univ. Algarve; UPTEC, Univ. Porto) with the aim of identifying industry needs
and offering potential solutions;
-
Continue and strengthen actions in the domain of training and outreach activities
through links with schools and active participation in programs such as, Ciência Viva,
summer studentships, and integration of undergraduates for short-term training
activities. More specific actions which are of relevance to the section and can have
elevated impact with better RL3 group integration are ventures into frontier science
which cross more than one research area, such as, i) isolation and characterization of
microalgae for biodiesel production; ii) development of chemical compounds and
screening on marine organisms; iii) reverse pharmacology - isolation and screening for
bioactive compounds from marine organisms; iv) development of in silico modeling
methods for prediction of protein: protein interaction.
39
RL4 - Aquaculture
1. Designation
Aquaculture
Maria Teresa Dinis
Marine Sciences
The objective of the Aquaculture Research Line is to promote the generation of high quality
scientific knowledge, through basic and applied research, to tackle some of the sustainability
challenges facing the aquaculture industry. The groups involved have reinforced their
collaboration during 2008 within the research areas previously defined:
-
Biological assessment of the Cultivation of New Aquaculture Species;
-
Fish Nutrition;
-
Fish Immunology and Health.
The collaboration links between the several research groups of RL4 (Aquaculture Research
Group; Nutrition, Growth and Quality of Fish Group (LANUCE); Fish Nutrition Group) have been
reinforced. Two joint research projects have started in 2008: OPTISOLE, a National SME
collaborative project aims to improve the resistance of sole to pathologies and TEXBREAM, a
FCT project, aims to determine the role of nutritional factors and farming practices as
modulators of flesh softening and aroma in farmed fish. Two other joint research projects, one
concerning the estimation of nutritional requirements for sole and blackspot seabream
(OPTIDIETAS) and another addressing the dietary modulation of the immune status in sole
juveniles (STRESSLING) ended in 2008. One project (SEACASE) is still running, aiming to develop
effective tools for the assessment of competitiveness and sustainability of extensive and semiintensive aquaculture production in Southern Europe. Additionally, 4 joint PhD projects have
been running.
Main scientific achievements include the optimization of dietary formulations and feeding
practices for blackspot seabream, Senegalese sole, white seabream, two banded seabream and
40
turbot. Immunostimulation strategies have been developed to enhance stress resistance and
immune response in Senegalese sole.
1. Collaborative publications in peer review journals (If more that one Research Groups of
Costas, B, Aragão, C, Mancera, JM, Dinis, MT, Conceição, LEC. 2008. High stocking density
induces crowding stress and affects amino acid metabolism in Senegalese sole Solea
senegalensis (Kaup 1858) juveniles. Aquaculture Research 39: 1-9.
2. Reis, PA, Valente, LMP, Almeida, CMR. 2008. A fast and simple methodology for
determination of yttrium as an inert market in digestibility studies. Food Chemistry 18(3): 10941098.
3. Rema, P, Conceição, LEC, Evers, F, Castro-Cunha, M, Dinis, MT, Dias, J. 2008. Optimal dietary
protein levels in juvenile Senegalese sole (Solea senegalensis). Aquaculture Nutrition 14: 263269.
4. Ribeiro, L, Couto, A, Olmedo, M, Alvarez-Blazquez, B, Linares F, Valente, LMP. 2008. Digestive
enzyme activity at different developmental stages of blackspot seabream, Pagellus bogaraveo
(Brunnich 1768). Aquaculture Research 39: 339-346.
5. Morais, J, Augusto, M, Carvalho, AP, Vale, P, Vasconcelos, VM. 2008. Cyanobacteria
hepatotoxins, microcystins: bioavailability in contaminated mussels exposed to different
environmental conditions. European Food Research and Technology 227: 949-952.
6. Silva, P, Rowlerson, AM, Valente, LMP, Olmedo, M, Monteiro, RAF, Rocha, E. 2008. Muscle
differentiation in blackspot seabream (Pagellus bogaraveo, Brunnich): histochemical and
immunohistochemical study of the fibre types. Tissue and Cell 40: 447-458.
2. Collaborative other publications (Include only if more than one group is involved and
only include here Books, chapters or full papers published in conference proceedings.
Give title and full citation in original language)
41
MASTER THESES
Aguiar Carvalho, CC. 2008. Physiological performance of the Peppery furrow shell Scrobicularia
plana (da Costa 1778) along the European coast. Master thesis in collaboration with the Royal
Netherlands Institute for Sea Research (NIOZ, Netherland), University of Trás-os-Montes and
Alto Douro (UTAD). Supervisor: Henk Van der Veer (NIOZ), Co-supervisor: P Rema (CIMARLANUCE).
Alves, R. 2008. Identificação de indicadores moleculares de bem-estar durante o cultivo de
dourada (Sparus aurata, Linnaeus 1758), utilizando técnicas de proteómica. Master thesis in
Marine Biology, University of Algarve. Supervisors: LEC Conceição (CIMAR-Aquagroup) and P
Rodrigues (CIMAR-EDGE).
Silva, P. 2008. Gnotobiotic growth system model for Dicentrarchus labrax larvae. Master thesis
in Marine Biology, University of Algarve. Supervisors: MT Dinis (CIMAR-Aquagroup) and P
Bossier (ARC).
Timóteo, V. 2008. Reprodução do pargo (Pagrus pagrus Linnaeus, 1758) em cativeiro- avaliação
da qualidade das posturas. Master thesis in Sciences of the Sea - Sea Resources, ICBAS,
University of Porto. Supervisors: MT Dinis (CIMAR-Aquagroup) and C Andrade (DRPM).
PhD THESES
Barbosa, A. 2008. A study of visual cues that elicit changeable camouflage in cuttlefish. PhD
thesis in Aquatic Sciences, ICBAS, University of Porto. In collaboration with the Marine Biology
Laboratory (MBL, USA). Supervisor: Roger Hanlon, Gil Rosenthal and Paulo Vaz-Pires (CIMARLANUCE).
Ribeiro, RA. 2008. Utilização de efluentes industriais (farinha de sangue e soro de leite) na
elaboração de ração para pirarucu (Arapaima gigas, Curvier.) PhD thesis in collaboration with
INPA (Brazil) and University of Acre (Brazil). Supervisor: Manuel Pereira (INPA), Rodrigo Ozório
(CIMAR-LANUCE).
Saavedra, M. 2008. Amino acid requirements of white seabream (Diplodus sargus) larvae:
effects on growth and performance. PhD thesis in collaboration with IPIMAR(Portugal).
Supervisors: MT Dinis and LEC Conceição (CIMAR-Aquagroup), Pedro Pousão-Ferreira (IPIMAR).
42
7d. Future research
The aquaculture industry in Portugal is suffering profound transformations. A massive increase
in production volumes with the off-shore cage producing systems is announced. But, there is
also a necessity to maintain competitiveness and profitability of semi-intensive aquaculture
production in earth ponds. Simultaneously, various new species will entry into commercial
farming phase of as a strategy to diversify the offer. Consumer awareness on the quality and
safety of seafood and on the sustainability of aquaculture as a food production system is also
growing. These evolving challenges have been identified as opportunities for research and
innovation that fall within the scope of CIMAR - RL4 attributions such as:
-
The development of new tools to assess muscle growth and final quality to combine
with the classic morphometric approach already implemented in CIMAR LA.
Understanding the molecular basis of environmental-induced phenotypic plasticity of
muscle development and growth will be a main research line.
-
The development of sensory schemes based on Quality Index Method, the most recent
sensory classification system for seafood items. In terms of physical methods, new
methods for the evaluation of rigor mortis will be developed, as well as others that
proved to be efficient and useful in quality and welfare evaluations, in addition to the
already used instrumental methods (Torrymeter and Freshmeter). In terms of
microbiology, the first studies will be focused on the characterization of the
microbiological environment of freshwater and seawater farms, including animals,
water and surfaces, and eventual resistances to antibiotics (antibioresistances) shown
by commensal (Escherichia coli and Enterococcus spp.) and pathogenic bacteria
(Salmonella sp.). Their ability to transmit those resistances to humans directly from the
consumption of farmed products and also by the aquatic environment will also be
considered.
-
To study the host/pathogen interaction in fish bacteriosis, aiming to understand the
mechanisms involved in resistance or susceptibility of fish species to some bacterial
pathogens and trying to develop methods of vaccination and/or immunostimulation.
-
To investigate the nutritional and metabolic utilization of nutrients in fish larvae and
juveniles. Activities on this area will also focus on the assessment of plant ingredientrich diets and farming practices as tools to enhance the quality, health benefits and
safety of farmed fish. Activities on the optimization of brood stock management through
improved nutrition and welfare, of innovative semen cryopreservation techniques and
work on the basis for brood stock selection programs for sole and grouper are also
foreseen. Besides classical zoo technical and biochemical assessment criteria, ongoing
and future projects will integrate also functional genomics, proteome expression, tracer
studies and metabolic modelling approaches. The access to functional genomic and
proteome expression platforms is already under implementation (in-house and external
collaborations).
43
-
Additionally, given its growing importance as a societal issue, the Fish Welfare research
line will be reinforced. Focus will be on basic knowledge regarding the nutritional
modulation of coping styles, stress resistance and health in fish, and the definition of
operational welfare indicators.
To be able to cope with this objectives industry contract research is absolutely required and
several projects have already been submitted to QREN. The already established of research
collaboration with both the feed industry (OPTIDIETAS, STRESSLING) and the aquaculture sector,
allows the rapid dissemination of results and the technological transference of results to
industry.
In terms of conference organization, AQUAGROUP hosted the IV Workshop The Cultivation of
Soles, in 11-13 November 2008. Members of AQUAGROUP, LANUCE and FISH NUTRITION
Laboratory participate in the organization of Aquaculture Europe 2010 to be held in Porto.
2. Future plans
The groups hosted by the CIMAR – RL4 will continue making efforts to reinforce their
partnership. Joint applications to four national research projects (FCT) are currently under
evaluation. Additionally, four joint PhD projects are currently underway. AQUAGROUP and
LANUCE members are also active participants in the European network COST Action FA0801
“LARVANET - Critical success factors for fish larval production in European Aquaculture: a
multidisciplinary network”. L Conceição is the Action Vice-Chair and together with L Valente are
the Portuguese representatives in the Management Committee.
For the future, the above Research groups pretend to assume a clear responsibility on the
establishment at CIMAR of a Fish Nutrition area under a common umbrella, the designated
CIMAR – Fish Nutrition Unit. In general terms, its mission is to promote and consolidate the
rationale development of the CIMAR expertise and activities in the area of Fish Nutrition, and
become an indispensable contributor in the elaboration of regional priorities for the sustainable
development of marine research.
44
8. RESEARCH GROUPS
1. Research group
Principal Investigator
Name of the Research Groups
Maria Teresa Dinis
Aquaculture Research Group (Aquagroup)
Isabel Maria Trigueiros Sousa Pinto Machado
Biodiversity of Aquatic Ecosystems
Maria Ester Tavares Alvares Serrao
Biogeographical Ecology and Evolution (BEE)
Maria Leonor Nunes Ribeiro Cruzeiro
Biophysics
João Carlos Serafim Varela
Biotechnology and Molecular Biology of
Microalgae (BMBM)
Manuel Aureliano Pereira Martins Alves
BioVanadium Research Group
Eduardo Jorge Sousa Rocha
Cellular and Molecular Studies (LECEM)
Maria Teresa Sá Dias de Vasconcelos
Chemical Speciation and Bioavailability (CS&B)
Adelino Vicente Mendonca Canario
Comparative Molecular Endocrinology (CME)
Luis Manuel Zambujal Chicharo
Ecology and Restoration of Estuarine and
Coastal Habitats (ECOREACH)
Ecophysiology
Lúcia Maria das Candeias Guilhermino
Ecotoxicology (ECOTOX)
Vitor Manuel Oliveira Vasconcelos
Ecotoxicology, Genomics and Evolution (LEGE)
Maria Clara Semedo da Silva Costa
Environmental Technologies
Maria Armanda Reis Henriques
Environmental Toxicology
45
Maria Leonor Quintais Cancela Fonseca
Molecular Biology of Marine Organisms
(MBMO), now consolidated as group EDGE
(Evolution, Development and Gene Expression)
Aires Manuel Pereira Oliva Teles
Fish Nutrition
José Pedro de Andrade e Silva Andrade
Fisheries Biology and Hydroecology Research
Group (FBHRG)
Karim Erzini
Fisheries, Biodiversity and Conservation
Geology and Paleoceanography
Adriano Agostinho Donas Bôto Bordalo e Sá
Hydrobiology
Rui Orlando Pimenta Santos
Marine Plant Ecology (ALGAE)
Luísa Maria Pinheiro Valente
Nutrition, Growth and Quality of Fish (LANUCE)
Maria Luisa Machado Cerqueira Bastos
Oceanic and Coastal Dynamics
Jorge Guimaraes da Costa Eiras
Pathology
Maria Lurdes Santos Cristiano
Synthesis and Organic Reactivity
46
Aquaculture Research Group (Aquagroup)
8a. Group description
1. Group name / denomination
Aquaculture research group (Aquagroup)
Maria Teresa Dinis
3. Location of group
CCMAR - Centro de Ciências do Mar
8b. Funding, sources, dates
In 2008, the Aquagroup was involved in 12 research projects, in most of them (11) with a
coordination role. Most projects (11) were funded through National agencies (FCT, ADI and
DGP-MARE), while 1 was undertaken by European funding (FP6). The total funds managed by
the Aquagroup in project running during 2008 attain 1.53 Million EUR (including overheads). It is
important to point out that 2008 was marked by the ending of 6 projects, but also by the
initiation of 5 new research projects.
73.056 EUR, FCT, DIGFISH, Jul 05 - Jun 08.
91.500 EUR, FCT, SULFAAR, Jul 05 - Jun 08.
55.404 EUR, FCT, SAARGO, Nov 05 - Oct 08.
56.923 EUR, ADI, OPTIDIETAS, Nov 05 - Jun 08.
181.374 EUR, ADI, STRESSLING, Jan 07 - Jun 08.
50.000 EUR, DGP-MARE, FISHWELL, Jan 07 - Sep 08.
312.088 EUR, EU-FP6, SEACASE, Jan 07 - Dec 09.
108.002 EUR, FCT, FATTYBONE, Aug 08 - Jul 11.
155.128 EUR, FCT, TEXBREAM, Aug 08 - Jul 11.
171.002 EUR, FCT, HYDRAA, Jul 08 - Jun 11.
189.785 EUR, FCT, CRYOSPERM, Apr 08 - Mar 11.
95.122 EUR, FCT, EFARFish, Oct 08 - Sep 11.
47
96.223 EUR, ADI, OPTISOLE, Sept 08 - Dez 11.
8c. Objectives and achievements
1. Objectives
The mission of the Aquagroup is to generate scientific knowledge, through basic and applied
research, to tackle some of the evolving sustainability challenges facing the aquaculture
industry. Our skills rely heavily on our expertise on broodstock management, larvae rearing and
nutrition of new species for Mediterranean aquaculture. The team assembles a range of
competencies that allow a multidisciplinary approach of several research themes, which have
been integrated in two key areas:
I. Nutrition for enhanced performance & quality
-
The nutritional and metabolic utilization of nutrients in marine fish larvae and juveniles.
Projects on this topic (DIGFISH, SULFAAR, SAARGO, HYDRAA, FATTYBONE, EFARFish)
comprise studies on the role of amino acids, peptide fractions and essential fatty acids
in areas such as digestive efficiency, intermediary metabolism, immune competence and
skeletal deformities in marine fish. Besides classical zootechnical and biochemical
assessment criteria, most projects integrate also functional genomics, proteome
expression, tracer studies and metabolic modeling approaches.
-
Broodstock Management of new species (sole, Dusky grouper) with a strong emphasis
on nutrition and welfare (FISHWELL) and the improvement of semen cryopreservation
success in various species (CRYOSPERM).
-
A new research topic on the Quality and Safety of Fish has been implemented. By
associating proteome analysis and traditional criteria, new knowledge will be generated
on the role of nutritional factors and farming practices as modulators of flesh softening
and aroma in farmed fish (TEXBREAM).
II. Sustainable practices
-
Research on the Production Systems topic have tackled aspects related to the
implementation of new aquaculture species through the estimation of nutritional
requirements for sole, blackspot seabream and white bream (OPTIDIETAS, SAARGO) and
the development of effective tools for the assessment of competitiveness and
sustainability of extensive and semi-intensive aquaculture production in Southern
Europe (SEACASE).
-
Research activities on Fish Welfare have also been reinforced. Issues addressed are the
dietary modulation of the immune status in sole juveniles (STRESSLING) and the
definition of operational welfare indicators in broodstock fish (FISHWELL).
-
Research on host/pathogen interactions mechanisms, establishment
immunostimulation strategies to enhance stress resistance and fish welfare.
48
of
Societal
-
Successful granting of 5 new research projects with coordination by Aquagroup
members.
-
Members of the Aquagroup have created a technology based spin-off company of
CCMAR devoted to the area of fish feed technology and nutrition.
Scientific
-
A set of 12 proteins have been identified as potential candidates for molecular
indicators of fish welfare, using a comparative proteomics approach.
-
Vitamin K supplementation of live feeds significantly reduces the incidence of skeletal
deformities in Senegalese sole larvae.
-
Optimized levels of antioxidant vitamins and beta-glucans were shown to enhance the
stress status and immune response in Senegalese sole juveniles.
-
A protocol for the reproduction of Dusky grouper has been further fine-tuned, with the
generation of 60000 larvae.
-
A practical diet for Senegalese sole juveniles has been optimized.
-
A practical diet with low fish-derived protein (13%) is able to sustain growth
performance and lower soluble phosphorus losses in gilthead seabream (Sparus aurata)
during the grow-out phase.
Career building for young researchers
Two post-doc researchers at the Aquagroup found stable careers elsewhere:
-
Ricardo Calado: Auxiliary Researcher at the CESAM Associated Laboratory (Portugal)
under the Ciência 2007 program.
-
Elsa Cabrita: Researcher at the ICMAN-CSIC (Spain) under the Ramon y Cajal program.
8d. Productivity
1. Publications in peer review journals
The Aquagroup has published a total of 21 ISI-listed peer-reviewed articles in the period 2008.
Many of these publications (48%) resulted from international collaborations. The mean Impact
factor (based on ISI JCR 2007) for those 21 publications has been 1.550, a value just within the
top 10 threshold value for the “Fisheries” subject category (ISI JCR 2007). From the 21 articles in
the period, 48% were published in this last subject category. From the total published articles,
49
19 and 10% were published in top 10 and top 20, respectively, journals of a subject category of
ISI JCR 2007. During 2008, the Aquagroup has published an average of 2.6 publications (ISI) per
year per Aquagroup member with a PhD degree.
Selected publications:
1. Aragão, C, Corte-Real, J, Costas, B, Dinis, MT, Conceição, LEC. 2008. Stress response and
changes in amino acid requirements in Senegalese sole (Solea senegalensis Kaup 1858). Amino
Acids 34: 143-148. IF=4.132.
2. Bartilotti, C, Calado, R, dos Santos, A. 2008. Complete larval development of the hermit crabs
Clibanarius aequabilis and Clibanarius erythropus (Decapoda: Anomura: Diogenidae), under
laboratory conditions, with a revision of the larval features of genus Clibanarius. Helgoland
Marine Research 62: 103-121. IF=1.591.
3. Beirão, J, Cabrita, E, Soares, F, Herraez, MP, Dinis, MT. 2008. Cellular damage in spermatozoa
from wild-captured Solea senegalensis as detected by two different assays: comet analysis and
Annexin V-Fluorescein staining. Journal of Applied Ichthyology 24: 508-513. IF=0.638.
4. Calado, R, Dinis, MT. 2008. Collection of marine invertebrates for the aquarium trade in
European waters: is anyone surveying? Aquatic Conservation – Marine & Freshwater
Ecosystems 18: 335-338. IF=1.500.
5. Calado, R, Dionisio, G, Bartilotti, C, Nunes, C, dos Santos, A, Dinis, MT. 2008. Importance of
light and larval morphology in starvation resistance and feeding ability of newly hatched marine
ornamental shrimps Lysmata spp. (Decapoda: Hippolytidae). Aquaculture 283: 56-63. IF=1.678.
6. Calado, R, Pimentel, T, Vitorino, A, Dionisio, G, Dinis, MT. 2008. Technical improvements of a
rearing system for the culture of decapod crustacean larvae, with emphasis on marine
ornamental species. Aquaculture 285: 264-269. IF=1.678.
7. Costas, B, Aragão, C, Mancera, JM, Dinis, MT, Conceição, LEC. 2008. High stocking density
induces crowding stress and affects amino acid metabolism in Senegalese sole Solea
senegalensis (Kaup 1858) juveniles. Aquaculture Research 39: 1-9. IF=0.991.
8. Martinez-Pastor, F, Cabrita, E, Soares, F, Anel, L, Dinis, MT, 2008. Multivariate cluster analysis
to study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts.
Reproduction 135: 449-459. IF=3.073.
9. Perez-Cerezales, S, Martinez-Paramo, S, Beirão, J, Cabrita, E, Herraez, MP. 2008. Analysis of
sperm DNA oxidation and fragmentation after cryopreservation in Sparus aurata. Biology of
Reproduction 78: 730. IF=3.469.
10. Rema, P, Conceição, LEC, Evers, F, Castro-Cunha, M, Dinis, MT, Dias, J, 2008. Optimal dietary
protein levels in juvenile Senegalese sole (Solea senegalensis). Aquaculture Nutrition 14: 263269. IF=1.398.
50
11. Saavedra, M, Conceição, LEC, Helland, S, Pousão-Ferreira, P, Dinis, MT. 2008. Effect of lysine
and tyrosine supplementation in the amino acid metabolism of Diplodus sargus larvae fed
rotifers. Aquaculture 284: 180-184. IF=1.678.
12. Saavedra, M, Conceição, LEC, Pousão-Ferreira, P, Dinis, MT. 2008. Metabolism of
tryptophan, methionine and arginine in Diplodus sargus larvae fed rotifers: effect of amino acid
supplementation. Amino Acids 35: 59-64. IF=4.132.
13. Calado, R, Bartilotti, C, Goy, JW, Dinis, MT. 2008. Parasitic castration of the stenopodid
shrimp Stenopus hispidus (Decapoda: Stenopodidae) induced by the bopyrid isopod Argeiopsis
inhacae (Isopoda: Bopyridae). Journal of the Marine Biological Association of the United
Kingdom 88: 307-309. IF=1.056.
14. Cunha, MC, Makridis, P, Soares, F, Rodrigues, P, Dinis, M.T. 2008. Timing of appearance of
lymphoid cells during early development of senegalese sole, Solea senegalensis Kaup. Journal of
the World Aquaculture Society 39: 436-439. IF=0.693.
15. Dos Santos, A, Calado, R, Araujo, R. 2008. First record of the genus Periclimenaeus
borradaile, 1815 (decapoda : palaemoniidae : pontoniinae) in the northeastern Atlantic, with the
description of a new species, Periclimenaeus aurae. Journal of Crustacean Biology 28: 156-166.
IF=1.109.
16. Makridis, P, Martins, S, Reis, J, Dinis, MT. 2008. Use of probiotic bacteria in the rearing of
Senegalese sole (Solea senegalensis) larvae. Aquaculture Research 39: 627-634. IF=0.991.
17. Rocha, RJ, Ribeiro, L, Costa, R, Dinis, MT. 2008. Does the presence of microalgae influence
fish larvae prey capture? Aquaculture Research 39: 362-369. IF=0.991.
18. Oliveira, JC, Soares, F, Engrola, S, Dopazo, CP, Bandín, I. 2008. Antemortem versus
postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis).
Journal of Veterinary Diagnostic Investigation 20: 215-219. IF=1.403.
19. Ribeiro, L, Couto, A, Olmedo, M, Álvarez-Blázquez, B, Linares, F, Valente, LMP. 2008.
Digestive enzyme activity at different developmental stages of the blackspot seabream, Pagellus
bogaraveo (Brunnich 1768). Aquaculture Research 39: 339-346. IF=0.991.
2. Other publications international (Books, chapters or full papers published in conference
proceedings)
Books (editors)
1. Calado, R. 2008. Marine Ornamental Shrimp - Biology, Aquaculture and Conservation. WileyBlackwell, UK. 263 pp.
2. Cabrita, E, Robles, V, Herraez, MP (Eds). 2008. Methods in Reproductive Aquaculture: Marine
and Freshwater Species. CRC Press, Boca Raton. 549 pp. ISBN 978-0-8493-8053-2.
51
Books (chapter)
1. Cabrita, E, Robles, V, Herráez, P. 2008. Sperm quality assessment. In: Cabrita, E, Robles, V,
Herráez, MP. (Eds). Methods in Reproductive Aquaculture: Marine and Freshwater Species. CRC
Press, Boca Raton, pp. 93-147.
2. Cabrita, E, Robles, V, Sarasquete, C, Herráez, P. 2008. Sperm cryopreservation from the
marine Teleost, Sparus aurata. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in
Reproductive Aquaculture: Marine and Freshwater Species. CRC Press, Boca Raton, pp. 447-451.
3. Conceição, LEC, Morais, SJ, Dinis, MT, Rønnestad, I. 2008. Tracer studies in fish larvae. In:
Cyrino, JEP, Bureau, D, Kapoor, BG (Eds). Feeding and Digestive Functions in Fishes. Science
Publishers, Enfield, New Hampshire, USA, pp. 349-392. ISBN 978-341-57808-57375-57806.
4. Herráez, P, Robles, V, Cabrita, E. 2008. Rainbow trout (Oncorhynchus mykiss) sperm
cryopreservation. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive
Aquaculture: Marine and Freshwater Species. CRC Press, Boca Raton, pp. 385-389.
5. Lahnsteiner, F, Soares, F, Ribeiro, L, Dinis, MT. 2008. Egg quality determination in teleost fish.
In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive Aquaculture: Marine and
Freshwater Species. CRC Press, Boca Raton, pp. 149-180.
6. Robles, V, Cabrita, E, Acker, JP, Herráez, P. 2008. Embryo cryopreservation:what we know
until now. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive Aquaculture:
Marine and Freshwater Species. CRC Press, Boca Raton, pp. 265-294.
7. Robles, V, Cabrita, E, Herráez, P. 2008. Sperm cryopreservation of sex-reversed rainbow trout
(Oncorhynchus mykiss). In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive
Aquaculture: Marine and Freshwater Species. CRC Press, Boca Raton, pp. 391-395.
3. Other publications national (Books, chapters or full papers published in conference
proceedings)
1. Dias, J, 2008. Douradas e robalos vegetarianos: ficção ou realidade? UALGzine.
2. Dinis, MT, Soares, F, Cabrita, E. 2008. Optimização da reprodução em linguado (Solea
senegalensis). In: DGPA (Ed). Investigação e Desenvolvimento no Sector das Pescas. Cadernos
PROMAR, pp. 71-74. ISBN: 978-972-98445-98444-98446.
4. Master and PhD theses completed
MASTER THESES
Alves, R. 2008. Identificação de indicadores moleculares de bem-estar durante o cultivo de
Marine Biology, University of Algarve. Supervisors: LEC Conceição and P Rodrigues.
52
Dionísio, G. 2008. Comportamento alimentar e resistência à inanação de larvas de camarões
ornamentais do Género Lysmata (Decapoda: Hippolytidae). Master thesis in Marine Biology,
University of Algarve. Supervisors: MT Dinis and R Calado.
Mota, EA. 2008. Evaluation of enzymatic and metabolic parameters of quality in sole eggs (Solea
senegalensis, Kaup, 1858). Master thesis in Marine Biology, University of Algarve. Supervisors:
MT Dinis, F Soares and E Cabrita.
Nunes, CJ. 2008. Optimização do cultivo momoclonal da anemona vidro Aiptasia pallida (Agassiz
in Veriil, 1864), com ênfase para a importância dos ácidos gordos. Master thesis in Marine
Biology, University of Algarve. Supervisors: MT Dinis and R Calado.
Rodrigues, VL. 2008. Nutritional modulation of innate immune parameters in the epidermal
mucus of Senegalese sole (Solea senegalensis). Master thesis in Marine Biology, University of
Algarve. Supervisors: MT Dinis, J Dias and L Ribeiro.
Silva, P. 2008. Gnotobiotic growth system model for Dicentrarchus labrax larvae. Master in
Marine Biology, University of Algarve. Supervisors: MT Dinis and P Bossier.
Timóteo, V. 2008. Reprodução do pargo (Pagrus pagrus Linnaeus, 1758) em cativeiro- avaliação
da qualidade das posturas. Master thesis in Sciences of the Sea - Sea Resources, ICBAS,
University of Porto. Supervisors: MT Dinis and C Andrade.
PhD THESES
Engrola, S. 2008. Improving growth performance of Senegalese sole postlarvae. PhD thesis,
University of Algarve. Supervisors: MT Dinis and LEC Conceição.
Saavedra, M. 2008. Amino acid requirements of white seabream (Diplodus sargus) larvae:
effects on growth and performance. PhD thesis in collaboration with IPIMAR (Portugal).
Supervisors: MT Dinis and LEC Conceição, Pedro Pousão-Ferreira (IPIMAR).
5. Patents/propotypes
6. Organization of conferences
-
4th Workshop on the Cultivation of Soles, 11-13 November 2008, CCMAR, Faro,
Portugal. (Organizers: B Howell, P Cañavate, R Prickett and L Conceição). The primary
aim of the Workshop was to evaluate the current status and future needs of the sole
farming industry. To this end, participants were invited from both the research and
commercial sectors whose expertise covers the wide range of subject areas on which
the future development of the industry will depend. The response to the meeting was
excellent with more than 80 participants from Europe and China attending the meeting.
-
Dissemination Workshop on aquaculture of new species, 19-23 March 2008, organized
by CCMAR and IPIMAR during the exhibit ExpoMar 2008, Olhão, Portugal. The aim of
this dissemination event was to present to the professional and general public the
53
progress obtained during the project PROMAR (INTERREG IIIA), on the cultivation of new
species for aquaculture, including fish and mollusks.
7. Industry contract research
8. Internationalization (Collaborative publication, Research, Graduate Training Networks
or other forms of participation of the Research Group at the international level)
The Aquaculture Research Group has a long standing tradition of associating itself to
international cooperation windows. Nine PhD thesis currently (2008) underway within the
Aquagroup benefit from structural collaborations with other research European research
institutions and universities, namely NIFES and University of Bergen (Norway), DFU (former
DIFRES, Denmark). This aspect is also reflected by the fact that 10 out of the 21 (48%)
publications of the Aquagroup in 2008 were written in collaboration with colleagues from
institutions outside Portugal.
Outside Europe, members of the Aquagroup have also been involved in scientific collaborations
with:
-
Cabo Verde - Teaching MSc disciplines at the University of Cabo Verde (MSc on Marine
Resources and Coastal Management. Collaboration was also extended to the Steering
Group on the reformulation of Marine Biology Courses.
-
Namibia - Training Staff of the Polytechnic of Namibi.
The Aquagroup members have also been active participants in the following networking
structures at European level:
-
COST Action 867 – Welfare of fish in European aquaculture. L Conceição is one of the
Portuguese representatives in the Management Committee.
-
European Aquaculture Technology and Innovation Platform (EATIP). J Dias is a member
of the working groups “Sustainable raw materials for fish feed production” and “Fish
health and welfare” devoted to the establishment of the Strategic Research Agenda.
-
COST Action FA0801 “LARVANET - Critical success factors for fish larval production in
European Aquaculture: a multidisciplinary network”. L Conceição is the Action ViceChair and one of the Portuguese representatives in the Management Committee.
As a result of its internationalization effort, the Aquagroup has been involved in three
applications to FP7 projects in 2008, of which one was approved (to start in 2009), and one went
up to the second stage of evaluation. One application was also submitted to POCTEP (former
INTERREG) together with Spanish colleagues, which was successful.
9. Government/organization contract research (Work carried out by the Research Group
that resulted in a publication or report. Of particular importance are those involved in
public policy advice)
54
8e. Future research
1. Objectives
The year 2008 was marked by the ending of 6 projects, but also by the initiation of 5 new
research projects (in the meanwhile 2 additional projects were granted and expected to start in
2009). Therefore, it was a period in which the Aquagroup felt the need to assess its performance
and evaluate its research strategy. The focus will be in two key areas:
-
Nutrition for Enhanced Performance & Quality
-
Sustainable Practices
The nutritional and metabolic utilization of nutrients in fish larvae, by means of the innovative
approach of tracer studies is a highly successful research topic, which gave the Aquagroup its
most distinctive character at both National and International level. This established research line
is to be further consolidated and broadened. Its development requires integrative studies on the
role of amino acids, peptide fractions, fatty acids and micronutrients. Activities on this area will
also focus on the assessment of plant ingredient-rich diets and farming practices as tools to
enhance the quality, health benefits and safety of farmed fish. Activities on the optimization of
broodstock management through improved nutrition and welfare, of innovative semen
cryopreservation techniques and work on the basis for broodstock selection programs for sole
and grouper are also foreseen. Besides classical zootechnical and biochemical assessment
criteria, ongoing and future projects will integrate also functional genomics, proteome
expression, tracer studies and metabolic modeling approaches. The access to functional
genomic and proteome expression platforms is already under implementation (in-house and
external collaborations). One new post-doc researcher and 4 new PhD students are now fully
dedicated to this area.
The aquaculture industry in Portugal faces the challenges of new off-shore cage farming
systems, and starting the commercial phase of several new species. Therefore, the Aquagroup
identified the Production Systems research area as a necessity and an opportunity to strengthen
its support to industry. Additionally, given its growing importance as a societal issue, the Fish
Welfare research line is to be reinforced. Focus will be on basic knowledge regarding the
nutritional modulation of coping styles, stress resistance and health in fish, and the definition of
operational welfare indicators. The implementation of this research area will be progressive
within CCMAR and in close collaboration with the Immunobiology Laboratory, from CIIMAR. One
new joint PhD student is already fully dedicated to this area.
The reinforcement of the collaborations in the area of Fish Nutrition within CIMAR LA, to gain
competitiveness in both National and International research consortiums, is a priority. The
following teams have taken in 2008 the decision to federate future research efforts on this area
under the umbrella of the new CIMAR LA – Fish Nutrition Unit:
-
Aquaculture Research Group at CCMAR, headed by Prof. Maria Teresa Dinis.
-
Fish Nutrition Group at CIIMAR, headed by Prof. Aires Oliva-Teles.
-
Laboratory of Fish Nutrition, Growth & Quality at CIIMAR, headed by Prof. Luísa Valente.
55
This Fish Nutrition Unit was recently granted a National SME collaborative project “Research and
development on the production of sole juveniles: enhanced resistance to pathologies and
growth” (Funding by QREN I&DT Co-Promoção, 2008-2011). Four additional joint research
projects are currently under evaluation (FCT). Additionally, 4 joint PhD projects are currently
underway.
2. Funding, source, dates (Amount of current and pending funding)

Current funding
FCT Pluriannual funding.
50.000 EUR, ECOAQUA, POCTEP, 2009-2010.
99.000 EUR, PROSPAWN, EU-FP7 RB-SME, 2009-2011.

Pending funding (approximately 2.4 Million EUR), for the period 2009-2012. Project
application short titles:
Ultrasound to enhance the nutritional status of eggs and yolk-feeding larvae.
Integrated studies on appetite regulation in gilthead seabream.
Understanding meagre larval digestive physiology through dietary modulation of some digestive
enzymes and metabolic processes during development.
Towards a better knowledge of taurine importance during fish ontogenesis.
Stress mitigation in sole through improved nutrition.
How does a sole broodstock with low stress response influence the quality of the progeny.
Manipulation of fish primordial germ cells: implications on Genetic Resource Banks and on
aquaculture reproductive management.
Biological basis for hatchery production of Dusky grouper for future restocking.
Molecular indicators of fish welfare.
The neural basis of fear processing in fish.
The accumulation of steroids in the water of Recirculating Aquaculture Systems.
Individuality of cognitive abilities in fish.
Dietary regulation of Senegalese sole lipid metabolism.
Development of eco-friendly rearing systems for marine fish larvae.
On the metabolism of the European cuttlefish.
56
Effects of dietary vitamin K supplementation on growth and fish skeleton.
Fish quality control: A proteomic approach and biomarker establishment.
Epigenetic regulation of development and growth in Senegalese sole.
Development of a reference diet for zebrafish.
Pilot project on the cultivation of meagre.
57
Isabel Maria Trigueiros Sousa Pinto Machado
CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto
Funds spent in 2008:
-
42.000 EUR, FCT Pluriannual funding.
-
National funding
4.199,67 EUR, FCT POCI/MAR/56149/2004, LIMITS.
24.370,20 EUR, FCT POCI/MAR758114/2004.
70.909,17 EUR, Ciência Viva, Sea Itinerant University.
-
European funding
21.881,11 EUR, 6th FP, BIOSTRAT.
44.984,07 EUR, 6th FP, BIODIVERSA.
24.353,42 EUR, 6th FP, MARBEF.
54.604,62 EUR, 5th FP, RACEWAYS.
-
Others
40.000 EUR, Parque Biológico de Gaia, 2008-2010.
4.000 EUR, Petrobrás e UFRN, 2008-2011.
57.000 EUR, Axa Foundation, 2008-2011.
58
1. Objectives
The general research objectives of the group are the study of biodiversity of marine ecosystems,
the promotion of their conservation and the development of sustainable use of their resources.
Within this framework our specific objectives are to:
-
Determine the patterns of distribution and abundance of species on benthic
communities and their variation at different spatial and temporal scales.
-
Develop and promote environmental monitoring schemes/long term data acquisition on
biodiversity and habitats and build and populate databases to be able to detect long
term biodiversity changes.
-
Determine the effects of different stressors on coastal biodiversity, detected from the
level of individual physiological responses to the functional effects at the ecosystem
level, and using different approaches: from observational studies to demographic
modelling and experimental manipulations of assemblages. This includes a particular
focus on the study of fish reproduction and behaviour, sexual selection and the
evolution of mating systems, and the use of animal behaviour as a proxy for detecting
xenobiotic contamination.
-
Develop Integrated Multitrophic Aquaculture systems (IMTAs), in collaboration with
existing commercial fish farms, by integrating the cultivation of algae and bacteria, that
have been previously isolated and characterized by the group, to produce economically
valuable biomass and improve water quality.
-
Investigate the bioactive secondary metabolites produced by different organisms,
including marine sponges, and to evaluate the in vitro anticancer and antifungal
activities of these metabolites.
-
Develop new products from marine organisms, and increase the value of species used
for improve water quality (IMTA systems) or discard organisms.
Besides these research objectives we also aim at:
-
Producing outreach programs and materials for the general public and school children.
-
Promoting research that is relevant to the conservation and sustainable use of marine
biodiversity.
-
Improve the effectiveness of communication and cooperation between researchers and
policy makers to facilitate the design and implementation of knowledge based policies
and action plans, at regional, National and European level.
59
During 2008 much effort was involved in the finalization and data analysis of European scale
projects under Marbef, the recruitment of 5 new researchers to strengthen our group and a
major effort in applying for research funding to develop our research objectives. We published
14 international publications and submitted several more.
In terms of research results during 2008 we:
-
Developed a molecular and multivariate approach to study the microbial community of
a commercial shallow-raceway-marine recirculation system operating with a Moving
Bed Biofilter.
-
Characterized the nitrification process and microbial dynamics in a commercial marine
raceway system with extended water recycling.
-
Selected seaweed species grown in integrated multitrophic aquaculture systems (IMTA)
to be ingredient for fish feed. Gracilaria and Porphyra gave the best results as
ingredients for trout and tilapia feed and continue to be produced for the next round of
longer term fish trials.
-
Developed a method to induce the formation of asexual spores for formation of new
gametophytes.to overcome the constraint of producing Porphyra
-
Tested several macroalgal new for cultivation. Stypocaulon and Grateloupia turuturu
showed good growth over a range of conditions and its nutrient uptake performance
was also tested in more detail.
-
Tested explicit hypotheses on the effects of loss of biodiversity on ecosystem
functioning at a pan-European scale and found that the strength of richness effects
rarely exceeded that of density-dependent processes in our experiments, but significant
interactions between both factors suggest that they should be always considered when
designing biodiversity experiments.
-
Developed and applied an array of behavioral and functional assays in order to address
the effects of xenobiotic exposure on fish life cycle. At a molecular level, we have also
isolated the VTG (I and II) genes from 3 fish species (L. pholis, S. abaster and N.
lumbriciformis), which are being used as model or sentinel species for disrupting
chemical contamination.
-
Broadened our understanding of fish reproductive biology, using syngnathids as model
species, by analysing the effects of OSR, mate availability and marsupium load, variables
taken as crucial for the expression of sex-role reversal.
60
8d. Productivity
Pollution 151(2): 408-413. IF=3.135.
2. orges, M-T, Sousa, A, De Marco, P, Matos, A, H nigov , P, Castro, PML. 2008. Aerobic and
anoxic growth and nitrate removal capacity of a marine denitrifying bacterium isolated from a
recirculation aquaculture system. Microbial Ecology 55(1): 107-118. IF=2.885.
3. Cerqueira, F, Cidade, H, van Ufford, L, Beukelman, C, Kijjoa, A, Nascimento, MSJ. 2008. The
natural prenylated flavone artelastin is an inhibitor of ROS and NO production. International
Immunopharmacology 8(4): 597-602. IF=2.157.
4. Kijjoa, A, Gonzalez, MJ, Pinto, MM, Nascimento, MSJ, Campos, N, Mondranondra, I-O, Silva,
AMS, Eaton, G, Herz, W. 2008. Cytotoxicity of prenylated xanthones and other constituents from
the wood of Garcinia merguensis. Planta Medica 74(8): 864-866. IF=1.960.
5. Masterson, P, Arenas, FA, Thompson, RC, Jenkins, SR. 2008. Interaction of top down and
bottom up factors in intertidal rockpools: Effects on early successional macroalgal community
composition, abundance and productivity. Journal of Experimental Marine Biology and Ecology
362:12-20. IF=2.074.
6. Nesshöver, C, Müssner, R, Henle, K, Sousa-Pinto, I. 2008. Linking biodiversity research and
policy in Europe. AMBIO 37:138-141. IF=2.092.
IF=3.135.
IF=0.659.
9. Pereira, R, Kraemer, G, Yarish, C, Sousa-Pinto, I. 2008. Nitrogen uptake by gametophytes of
Porphyra dioica (Bangiales, Rhodophyta) under controlled-culture conditions. European Journal
of Phycology 43(1): 107-118. IF=1.826.
10. Sawangwong, P, Wattanadilok, R, Kijjoa, A, Silva, AMS, Eaton, G, Herz, W. 2008. Secondary
metabolites from a marine sponge Cliona patera. Biochemical Systematics and Ecology 36(5-6):
493-496. IF=1.136.
11. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2008. Can the limited marsupium space be a
limiting factor for Syngnathus abaster females? Insights from a population with size-assortative
mating. Journal of Animal Ecology 77(2): 390-394. IF=4.220.
61
12. Vaselli, S, Bertocci, I, Maggi, E, Benedetti-Cecchi, L. 2008. Effects of mean intensity and
temporal variance of sediment scouring events on assemblages of rocky shore. Marine Ecology
Progress Series 364: 57-66. IF=2.631.
1 . Viejo, RM, Arenas, F, Fern ndez, C, mez, M. 2008. Mechanisms of succession along the
emersion gradient in intertidal rocky shore assemblages. Oikos 117(3): 376-389. IF=2.970.
proceedings)
1. Yarish, C, Pereira, R. 2008. Mass Production of Marine Macroalgae. In: Sven Erik Jørgensen
and Brian D. Fath (Editor-in-Chief). Ecological Engineering. Vol. [3] of Encyclopedia of Ecology, 5
vols, Oxford: Elsevier, pp 2236-2247.
proceedings)
1. Vieira, R, Pereira, R, Arenas, F, Araújo, R, Sousa Pinto, I. 2008. Guia de Campo das Espécies
Intertidais Características da Costa Norte de Portugal - Praias Rochosas. CIMAR, Porto.
2. Peixe, C, Pereira, CM, Silva, F, Borges, M-T, 2008. Sensor for amperometric determination of
ammonia in seawater. IJUP08 - Young Researchers of University of Porto Meeting, Porto, 20-22
February. Abstract Book p. 127.
3. Matos, A, Borges, M-T, Castro, PML. 2008. Microbial community distribution and dynamics in
a shallow raceway recirculating mariculture system. IJUP08 - Young Researchers of University of
Porto Meeting, Porto, 20-22 February. Abstract Book p. 193.
MASTER THESES
Peixoto, MJ. 2008. Avaliação da qualidade biológica da água do rio Cávado. Master thesis in
Hydrobiology, Faculty of Sciences, University of Porto. Supervisor: MN Vieira.
Martins, AS. 2008. Os macroinvertebrados bentónicos como indicadores da qualidade da água
de duas ribeiras (Ribeira da Madalena e Ribeira de Canelas). Master thesis in Hydrobiology,
Faculty of Sciences, University of Porto. Supervisor: MN Vieira.
Coelho, E. 2008. Avaliação da qualidade ecológica do rio Leça. Master in thesis Hydrobiology,
Faculty of Sciences, University of Porto. Supervisor: MN Vieira.
Nunes, AI. 2008. Qualidade da água residual - monitorização através de respostas ecológicas de
protozoários e remoção de amónia e azoto total utilizando Chlorella vulgaris. Master thesis,
Faculty of Sciences, University of Porto.
62
PhD THESES
Silva, C. 2008. Reproductive Ecology of the mildly sex-role reversed pipefish, Syngnathus abaster
(Pisces; Syngnathidae). PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor:
MN Vieira, Vitor Almada (ISPA).
A Kijoa is the organiser of the 6th European Conference on Marine Natural Products, to be held
in July 2009.
I Sousa Pinto is member of the Scientific Committee of the 2nd European Congress of
Conservation Biologists to be held in Prague, September 2009.
Our group continued to work extensively with colleagues from Europe and around the world
participating in EU framework projects and being part of the steering, executive or management
Committees of Biostrat, (32 countries), NoE Marbef with 56 partners and Era-Net Biodiversa. We
have also participate in ENCORA, a European coastal network, and in Naguisa (CoML) an
international project, and several bi-lateral projects with Spain, Brazil and Thailand, that often
include co-advisor ship of students.
We participate in the Erasmus Mundus MSc in Marine Biodiversity and Conservation with
Universities from Belgium, Spain, Lithuania, Portugal, France.
We continue to participate in several European scale experiments: e-g- on effects of biodiversity
on the functioning and stability of marine ecosystems, or to identify the intensity, timing, and
temporal extent of production and settlement of pelagic propagules of benthic organisms at a
Pan-European scale. Experiments are finished and papers are being written and the first were
already submitted to publication on Ecology, Ecology Letters and Hydrobiologia.
We started a new project determining the efficiency of new antifouling paints around different
European harbours with a Swedish company.
Participated in the work of Biodiversa: Era-Net on Biodiversity.
Furthermore I Sousa Pinto is member of the:
-
Editorial oard of the new” Journal of Marine iodiversity”, Springer.
63
-
Study Group on Biodiversity Science (SG BioDiv) from ICES.
-
Steering Committee of EPBRS (European Platform for Biodiversity Research Strategy).
-
Steering committee of the International Program “Census of Marine Life (CoML)” and
member of the “2020 Science Council” to plan the continuation of this program after
2010.
-
Board of Directors of the “Society for Conservation iology” Europe.
-
Executive Board of MARS - European Network of Marine Research Institutes and
Stations.
I Sousa Pinto continued to serve in the Portuguese delegation the Convention for Biological
Diversity (since 2006), representing the Ministry of Environment. During 2008 participated in the
9th SBSTTA in Rome and in the Conference of the Parties (COP) in Bonn. I Sousa Pinto was also
nominated focal point from the same Ministry to participate in the project MARBIS that was
coordinated by the Ministry of Environment, Ministry of Defense and Marine affairs and
Ministry of Research.
8e. Future research
1. Objectives
The first objective is to continue to increase the synergies between present and the new
researchers that are being hired to strengthen our research on biodiversity. The general
research objectives of the group will continue to be the study of different aspects of marine
biodiversity and its role on coastal aquatic ecosystems, and the promotion of the sustainable
use of marine resources. We will further integrate our research with other groups at national
and international level, to be able to do more interdisciplinary research. We’ll continue to
increase our inputs for global databases (Algabase, GBIF, OBIS), international research programs
(CoML and its successor 2020) and national initiatives as the network for Marine and Coastal
Biodiversity that we founded in 2007.
The integration next year of a new member with very high taxonomic skills on marine
crustaceans will greatly increase our ability to identify and quantify the biodiversity that is
present in our waters, as well as to contribute to the global development of taxonomy,
especially in marine crustaceans and on Tanaidacea.
The integration of a new member with modelling capabilities will allow developing and
implementing hydrodynamic and biogeochemical models, to integrate knowledge on coastal
ecosystems and develop decision support systems that can be used as management tools.
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We also aim to contribute with know how to the implementation of environmental and marine
policies e.g. the EU Water Framework Directive (new project EEMA) and the Habitats Directive
for the marine environment, as well as participating in the development of methodologies for
implementation of other EU Directives, as the Marine Strategy Directive and ICZM.
Within this framework we will work further on:
-
Patterns of distribution and abundance of benthic species, and effects of climate change
(2 pending projects), focusing more on population genetics and subtidal assemblages,
increasing our capability by hiring a new researcher on ecology of subtidal coastal
ecosystems and one on population genetics.
-
Effects of different stressors. To strengthen this line we will hire two new researchers
with experience on analysis of effects of stressors on ecosystem functioning: one in
rocky shore communities and other in soft bottom communities.
-
Invasive species to evaluate the need for management actions and start a survey of
ports and marinas to detect introduced species and evaluate their potential for
becoming invasive (Pending project and new AXA project).
-
Fish ecology and behaviour, studying the influence of climate change on the expression
of sexual selection pressure and alteration of fish mating systems.
-
Use of test organisms for assessment of wastewater toxicity and developing
methodologies to rehabilitate degraded water bodies.
-
Development of new products form marine organisms and IMTA systems including: new
products form algae, e.g. biofuels, biomedical compounds (ongoing PhD and new
IBEROMARE project) and as ingredient for fish feed (Post-doctoral project).
-
Isolation and structure elucidation of the secondary metabolites of soil fungi and higher
plants, and research on bioactive secondary metabolites from marine fungi including
endophytic fungi from marine sponges and from mangroves.
-
Evaluation of the isolated compounds for their in vitro anticancer and antifungal
activities and study of the underlying mechanisms.
2.
Funding, source, dates (Amount of current and pending funding)

Current funding
37.064 EUR, QREN-POVT, EEMA Project, 2008-2011.
107.000 EUR, 6th FP, MARBEF, 2004-2009.
203.440 EUR, 6th FP, BIODIVERSA, 2006-2010.
65
148.500 EUR; 6th FP, BIOSTRAT, 2006-2010.
100.000 EUR, INTERREG IV POCTEP, IBEROMAR Project, 2009-2011.
-
Others
40.000 EUR, Parque Biológico de Gaia, 2008-2010.
4.000 EUR, Petrobrás e UFRN, 2008-2011.
57.000 EUR, Axa Foundation, 2008-2011.
60.000 EUR, Área Metropolitana do Porto, 2009-2011.

Pending funding
100.000 EUR, FCT PTDC/MAR/105229/2008, Benefits.
120.000 EUR, FCT PTDC/MAR/104212/2008, GreenMount.
80.000 EUR, FCT PTDC/MAR/103967/2008, Development of a benthic chamber for rocky
substrates.
95.000 EUR, FCT PTDC/MAR/105211/2008, FUNDIN.
85.000 EUR, FCT PTDC/MAR/105147/2008, PHYSIOGRAPHY.
143.000 EUR, FCT PTDC/AAC-AMB/105244/2008, Aquamonitor. Coordinator: INESC-Porto.
FCT PTDC/MAR/100482/2008, AzoAlg.
FCT PTDC/AAC-CLI/100908/2008.
FCT PTDC/MAR/108369/2008.
FCT PTDC/MAR/108947/2008.
66
Biogeographical Ecology and Evolution (BEE)
Biogeographical Ecology and Evolution
Maria Ester Tavares Alvares Serrao
CCMAR - Centro de Ciências do Mar, Faro
Funds indicated are those in projects that were in place in 2008, amount shown is only that for
the team BEE.
-
EU funds
891.160 EUR, LIFE 06 NAT/P/000192, BIOMARES, 2007-2010.
295.908 EUR, NEST-2005-Path-COM/043251. EDEN, 2007-2009.
32.500 EUR, ESF-EUROCORES 1390, DEECON, 2005-2009.
Plus various projects in MARBEF and MGE networks.
-
FCT
As coordinators:
86.400 EUR, POCI/MAR/61105/2004, ADAPT, 2006-2009.
82.800 EUR, POCI/MAR/60179/2004, DIVSTAB, 2006-2009.
89.100 EUR, POCI/MAR/57499/2004, MATING, 2006-2008.
41.400 EUR, POCI/MAR/60044/2004, DIVIDE, 2005-2008.
As partners:
32.500 EUR, POCI/MAR/58837/2004, Eel life history, 2005-2008.
25.020 EUR, POCI/MAR/56149/2004, LIMITS, 2006-2009.
67
35.138 EUR, PTDC/BIA-BDE/74349/2006, HERCULES, 2008-2011.
-
Subcontracts
30.000 EUR, ECOKELP, Agence Nationale Recherche, France, 2007-2009.
8.500 EUR, Inst. Canario Ciencias Marinas, Spain, 2006-2009.
1. Objectives
We aim at understanding patterns and processes mediating population biology from ecological
to evolutionary scales. Topics include phylogeography, population dispersal/connectivity, abiotic
stress-driven evolution, reproductive ecology and roles of mating systems in population
divergence and speciation. Biological models include marine plants, algae, animals, using a
variety of approaches, from molecular to experimental ecology and phylogenetic reconstruction.
A large focus of current research is the central question in evolutionary ecology of the nature of
environmental barriers that limit gene flow and induce population genetic divergence, a first
step towards speciation. We identify gene flow barriers in marine populations, a subject
particularly poorly understood for marine taxa given the apparent continuity of marine
environments for dispersal, and we track genetic imprints of ocean-climate cycles (lower
temperatures and sea levels) from Pleistocene ice ages, on several taxa of pelagic and benthic
marine species across various oceans and seas, inferred from genetic structure and molecular
phylogenies.
In biogeographical theory we also study evolutionary patterns of archipelago colonizations and
we empirically test theoretical predictions from evolutionary ecology of species´ranges,
whereby distributional edges and marginal habitats would be expected to have higher clonality
and inbreeding, selected for reproductive assurance and local adaptation, and higher genetic
differentiation and lower genetic diversity which trade-off with higher selective pressures for
stress-driven local adaptation at range edges.
We address leading questions in marine ecology, such as maximizing fertilization success in
exposed shores by timing broadcast spawning synchrony, comparing population mating systems
in order to understand their roles in enabling species to respond to variable conditions and
eventually driving reproductive isolation and speciation, maintaining species integrity in sister
taxa that can hybridize and introgress.
These objectives have required significant effort into methodological objectives, including
development of various types of molecular methods and molecular markers, novel population
genetics analyses methods, and specific software.
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12 papers in 2008.
Evolutionary histories (phylogenies), population structure and barriers to gene flow were
identified, revealing genetic imprints of Pleistocene glacial periods on several pelagic and
benthic species, across Atlantic and Mediterranean basins. Marks of displacements, vicariance,
extinctions/recolonizations and contemporary gene flow restrictions were revealed in fish, algae
and seagrass species.
Independent colonizations events with remarkably recuring biogeographical patterns of
diversification were discovered in endemic gastropods (Conus) in Cape Verde archipelago.
Complex network analysis based on genetic markers revealed population nodes that most
contribute to population connectivity in a Mediterranean seagrass.
Temporal spawning patterns were identified in fucoid algae, revealing higher synchrony in
dioecious than in hermaphrodite taxa, and contrasting periodicity in an invasive versus a native
species from the same habitat.
Signatures of glacial refugia and adaptation across environmental gradients were reviewed for
Atlantic marine taxa.
Molecular phylogenies were used to understand the evolutionary history of cicada songs
revealing both consistent character evolution and discrepancies caused by species-specific
selection.
Novel molecular markers were developed for population genetics of species in endangered
habitats (temporary ponds).
8d. Productivity
1. Alberto, F, Massa, S, Manent, P, Diaz-Almela, E, Arnaud-Haond, S, Duarte, CM, Serrão, EA.
2008. Genetic differentiation and secondary contact zone in the seagrass Cymodocea nodosa
across the Mediterranean-Atlantic transition region. Journal of Biogeography 35: 1279-1294.
IF=4.566.
2. Barata, M, Harris, DJ, Castilho, R. 2008. Variation within North African Natrix maura
(Serpentes: Colubridae) inferred from mtDNA sequences: comparative phylogeography of
Magrehbian reptiles. African Zoology 43:1-7. IF=0.437.
3. Cunha, RL, Tenório, MJ, Afonso, C, Castilho, R, Zardoya, R. 2008. Replaying the tape: recurring
biogeographical patterns in Cape Verde Conus after 12 million years. Molecular Ecology 17: 885901. IF=5.325.
69
Phycology 43: 275-282. IF=1.826.
mitochondrial DNA (Insecta, Hemiptera). Bioacoustics 18: 17-34. IF=0.722.
6. Francisco, SM, Congiu, L, Stefanni, S, Castilho, R, Brito, A, Ivanova, PP, Levy, A, Cabral, A, Kilias,
G, Almada, VC. 2008. Phylogenetic relationships of the North-eastern Atlantic and
Mediterranean forms of Atherina (Pisces, Atherinidae). Molecular Phylogenetics and Evolution
48: 782-788. IF=3.871.
7. Ladah, L, Feddersen, F, Serrao, EA, Pearson, GA. 2008. Egg release and settlement patterns of
a dioecious and hermaphrodite fucoid algae during the tidal cycle. Marine Biology 155: 583-591.
IF=1.953.
8. Maggs, CA, Castilho, R, Foltz, D, Henzler, C, Jolly, T, Kelly, J, Olsen, J, Perez, KE, Stam, W,
Vainola, R, Viard, F, Wares, J. 2008. Evaluating signatures of glacial refugia for North Atlantic
Marine Organims. Ecology 89 (sp11): S108-S122. IF=4.874.
9. Rozenfeld, AF, Arnaud-Haond, S, Hernández-García, E, Eguíluz, VM, Serrao, EA, Duarte, CM.
2008. Network analysis identified weak and strong links in a metapopulation system.
Proceedings of the National Academy of Sciences of the USA. 105: 18824-18829. IF=9.380.
10. Schmidt, PS, Serrao, EA, Pearson, GA, Riginos, C, Rawson, PD, Hibish, TJ, Brawley, SH,
Trussell, GC, Carrington, E, Wethey, DS, Grahame, JW, Bonhomme, F, Rand, DM. 2008.
Ecological genetics in the North Atlantic intertidal: environmental gradients, replicated clines,
and adaptation at specific loci. Ecology 89(sp11): S91-S107. IF=4.874.
11. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2008. Development and
characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad,
Pelobates cultripes. Conservation Genetics, doi:10.1007/s10592-008-9670-6. IF=2.408.
12. Van de Vliet, MS, Diekmann, OE, Serrão, ETA, Beja, P. 2008. Highly polymorphic
microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for
cross-species amplification. Conservation Genetics, doi:10.1007/s10592-008-9609. IF=2.408.
proceedings)
proceedings)
70
MASTER THESES
Fragoso, B. 2008. Assessment of biofouling in PVC recruitment surfaces and efficiency of non
toxic materials in the construction of nets in an oyster aquaculture farm in Sagres. Master thesis
in Marine Biology, University of Algarve. Supervisor: E Serrão, John Icely.
Bayesian Evolutionary Analysis Sampling Trees - BEAST, 29-30 May 2008. Lecturers: Philippe
Lemey (Leuven University, Belgium) and Beth Shapiro (Pennsylvania State University, USA). 22
international participants.
EU Networks of Excellence:
-
MARBEF (Marine Biodiversity and Ecosystem Function).
-
MGE (Marine Genomics Europe).
-
Our team participation in the MARBEF network seeded the participation of the
University of Algarve as core-partner in the EU Erasmus-Mundus MSc program Marine
Biodiversity and Conservation.
Referee work by team members:
-
G Pearson - Associated editor, European Journal of Phycology.
-
Revision of manuscripts for >30 international journals
-
Revision of international research proposals for NSF, USA.
International graduate training:
-
Three PhD students under joint degree agreements Univ.Algarve-Univ.ParisVI (E Billard,
J Neiva, Tânia Pereira).
-
All our Portuguese PhD students have international co-supervisors and develop part of
their research in foreign institutions, including in the USA, France, Spain, and Belgium.
71
Joint international publications:
-
Most of our research (see publications) includes international collaborations with other
international teams.
Our novel projects involve international collaborations that make it logistically possible to study
various marine ecosystems throughout the world. Examples:
-
Collaboration with CSIC,Spain (Carlos Duarte, Susana Augusti) allow us access to polar
research cruises and laboratory and shipboard experimental set-ups for our studies of
phytoplankton transcriptomics stress-responses.
-
Collaborations with Univ.California (Pete Raimondi, Daniel Reed) provide us access to
long term ecological research GIS data (LTER-SBC) on giant kelp Macrocystis pyrifera, to
overlap with our gene flow data and describe landscape genetics of giant marine forests,
whereby we will contribute to design the first managed network of marine protected
areas (MPAs) for the state of California.
-
Collaboration with IFREMER, Brest (team of Daniel Desbruyères) provides us access to
samples of shrimp from deep sea vents throughout the world’s ridges, and
characterization of their associated bacteria for co-evolution questions.
BIOMARES – Restoration and management of biodiversity in the marine Park Site ArrabidaEspichel. Multidisciplinary project aimed at contributing to help management policies in a
Marine Park. Coordinated by both teams BEE and CFRG of CCMAR.
http://www.ccmar.ualg.pt/biomares/
FIND*KELP – Finding and mapping kelp with community participation. Fund by the AWARE
Foundation Europe. Project based on community participation by amateur scuba divers that
contribute to mapping and monitoring of Portuguese subtidal marine florests.
http://www.findkelp.org/
8e. Future research
1. Objectives
Besides continuation of past research objectives outlined above, we highlight here novel
research objectives in newly funded projects:
Phylogeography, edge population genetics
72
-
The level and influence of Pleistocene gene flow between North Africa and Europe
(mainly Iberia and Southern Italy) will be determined in both invertebrate and
vertebrate organisms with different colonizing abilities.
-
Phylogeography and historical demography will be compared at retreating edges and
leading edges of cold temperate and warm water organisms respectively, in coastal
European waters. Main focus will be on key canopy-forming habitat structuring species
and associated animal taxa.
Genetics of metapopulation systems
-
Metapopulation genetics of forests of giant kelp Macrocystis pyrifera, that sustain one
of the most diverse, productive, and dynamic ecosystems on the planet, will unravel
gene-flow patterns at different spatial scales, from intra- to metapopulation scales in
California, to global ocean biogeography, including various Pacific biogeographic
boundaries and effects of the west wind drift that connects Pacific, Atlantic, and Indian
ocean along the Southern Ocean.
-
Gene flow analyses within and between patches of Iberian kelp forests will infer
reproductive ecology and assess population fragmentation.
-
Seagrass habitat fragmentation effects assessed on genetic structure and gene flow of
associated seahorses and pipefishes that depend largely on seagrass habitat.
Deep sea connectivity
-
Spatial distribution of intraspecific biodiversity in bony fish and sharks will be studied
along continental slopes and Mid-Atlantic Ridge. Integrating genetic, phenotypic and
oceanographic data will unravel processes shaping population connectivity patterns in
the deep-sea, contributig to management of deep-sea fisheries and other
anthropogenic pressures.
-
Phylogeny and phylogeography of deep-sea shrimp (Alvinocarididae) across Atlantic and
Pacific deep-sea chemosynthetic ecosystems will trace back colonization pathways
along ridges.
Invasive species genetics and bacterial interactions
-
The evolution of invasive Caulerpa species and its associated bacterial flora will be
compared in Australian (native range) and Mediterranean (invaded range) coasts,
assessing also the possible flow of associated microorganisms with the native
congeneric species of the Mediterranean.
Polar phytoplankton stress-responses
-
In the Southern Ocean, which disproportionately influences global climate, genomewide patterns of stress-responsive gene expression will be characterized in natural
phytoplanktonic assemblages with key roles in macronutrient fluxes and export of fixed
carbon. In polar research cruises, experiments will be conducted with natural
73
phytoplanktonic assemblages, in which major hypothesized bottom-up drivers of
ecosystem function will be manipulated (Fe, Si, UVR, light).
-
Genomic responses near climate-induced tipping points for Arctic phytoplankton will be
assessed as short-term responses of phytoplankton community transcriptome in situ
during bloom-forming events, and for selected major bloom-forming diatoms (basis of
Arctic food-webs). Deep transcriptome coverage in both polar projects will be achieved
using next generation high-throughput pyrosequencing of cDNA libraries.
2.

Current funding
190.672 EUR, FP7-ENV-2008-1-226248, Arctic Tipping Points - ATP, 2009-2012. PI: P
Wassmann,Univ. Tromsø; PI: G Pearson (at EU negotiation stage).
78.000 EUR, MarinERA-189570, Marine phylogeographic structuring during climate change: the
signature of leading and rear edge of range shifting populations - SHIFTING, 2008-2011. PI:
VAlmada, ISPA.
17.500 EUR, EuroCores 1 390, Unravelling population connectivity for sustainable fisheries in the
Deep Sea - DEECON, 2007-2009. PI: NC Stenseth.
190.672 EUR, PTDC/MAR/72630/2006, Environmental Genomics of Southern Ocean
Phytoplankton Communities, 2008-2011. PI: G Pearson.
191.441 EUR, PTDC/MAR/65461/2006, Molecular ecology of the giant kelp Macrocystis pyrifera,
2008-2011, PI: F Alberto.
182.630 EUR, PTDC/MAR/70921/2006, Population genetic structure, molecular phylogeny and
historical biogeography in species of the genus Caulerpa, 2008-2011. PI: E Varela.
187.140 EUR, PTDC/MAR/64749/2006, The success of invasive species: exploring the joint role
of biotic interactions and of founder effect in Caulerpa species, 2008-2011. PI: S Arnaud.
35.138 EUR, PTDC/BIA-BDE/74349/2006. Gene flow across the pillars of Hercules: the role of
North Africa in the postglacial recolonization of Europe – HERCULES, 2008-2010. PI: J Harris.
5.3648 EUR, PTDC/BIA-BDE/68730/2006, Spatial structure of amphibian (meta)populations in
Mediterranean farmland, 2008-2010. PI: P Beja,CIBIO.
Full genome sequencing of Zostera marina. PI: J Olsen, core partner E Serrao. Joint Genome
Institute (JGI) -Community Sequencing Program (CSP2009).
3.200 EUR, FINDKELP, AWARE Foundation Europe.
74
Biophysics
Biophysics
Maria Leonor Nunes Ribeiro Cruzeiro
70.000 EUR. PI: L Cruzeiro for two tasks in the research project “Nonlinear waves in discrete,
periodic and quasiperiodic systems”. Funded by FCT, 2005-2008.
ca 23.340 EUR/year. Postdoctoral grant for Dr. Holly Freedman, funded by FCT, ref.
SFRH/BPD/41143/2007.
1. Objectives
The ultimate aim of the Biophysics Group is to understand how proteins fold and also how they
function. Proteins are the machines of life, they mediate most processes that take place in a cell,
and understanding how they function is not only of interest for Marine Biology but also for
Biology in general, as well as for Biomedicine, for Biotechnology and for the Pharmaceutical
Sciences.
Our scientific approach is based on two fundamental assumptions: a) the free energy landscape
of proteins is multi-funnel shaped and b) protein folding and function involve the storing and
propagation of energy in the form of vibrational excited states (VES), something that we have
started to designate as the VES hypothesis. The vibrational state we are mostly interested in is
the amide I vibration, whose storing and transport from carbonyl to carbonyl is described by the
Davydov/Scott model.
In recent years the research work was developed along four lines: 1) application of the
Davydov/Scott model for energy transfer in proteins to the interaction of proteins with
electromagnetic radiation; 2) application of the VES hypothesis to the structural instability of
prions and other proteins involved in misfolding diseases; 3) extension of the Davydov/Scott
model to a model that does not conserve the amide I number, in order to explain the generation
75
of conformational changes; 4) testing of the assumption a) above through computer
simulations.
An extra, 5th, line of research pursued was the investigation of the stabilization of electron pairs
by vibrational modes of nonlinear lattices, a topic that mixes the Davydov/Scott model with the
Hubbard model that describes electron correlations in condensed matter systems. Although the
direct interest here is the mechanism of High Temperature Superconductivity, these models
may in the future prove useful also for electron transfer in proteins.
In 2008 the research activities were concentrated on the lines of research 1), 2), 4), and 5)
described in item Objectives. Specifically, regarding line 1) it was found that positive dipoledipole couplings between the amide I modes can lead to absorption line shapes with double
peaks whose relative intensity varies with temperature, as is found in the organic crystal of
Acetanilide, in which these molecules form hydrogen bonded chains similar to those that
stabilize alpha-helices and beta sheets (Cruzeiro L. 2008. J. Phys. B: At. Mol. Opt. Phys. 41:
195401; Cruzeiro L. 2009. J. Biol. Phys. 35: 43-55).
Concerning line 2), the previously extended Davydov/Scott model was applied to the transfer of
energy from the bending mode of water to the amide I vibrations of an alpha-helix. It was found
that when all the amino acids of that helix are glutamine or asparagine the amount of energy
extracted from water is up to fifteen times greater, something that can help explain the greater
instability of huntingtin (Cruzeiro L. 2008. J. Phys. Org. Chem. 21: 549-554).
Concerning the fourth research line specified in Objectives, four proteins were selected to
represent each of the four different CATH classes and, for each protein, three decoys were built
with structures that had a RMSD of 10 Å to 15 Å relative to the native structure. The decoys
were scored against the native structure with the help of the AMBER force field, using three
measures: the average energy, the average fluctuation and the resistance to a heat pulse. From
50 nanosecond long MD simulations the overall conclusion is that of these three measures that
which is most successful in picking out the native structures is the last one since the native
structures take a consistently longer time to be destabilized in this manner. But the general
conclusion is also that none of measures is completely effective in discriminating all the decoys
from the native structure, a result that supports other studies according to which the native
state is reached by a kinetic step (Cruzeiro L and Lopes P.A. Are the native states of proteins
kinetic traps? Mol. Phys, accepted).
Concerning the fifth line of research a new collaboration was started, with the group of Prof.
Manuel G Velarde (Instituto Pluridisciplinar of the Universidad Complutense, Madrid, Spain),
with the aim of exploring the solectron concept, recently proposed by MG Velarde as another
mechanism for electron conduction in condensed matter and which relies on the intrinsic
nonlinear properties of the lattice.
76
8d. Productivity
1. Cruzeiro L. 2008. Protein's multi-funnel energy landscape and misfolding diseases. Journal of
Physical Organic Chemistry 21: 549-554. IF=1.415
2. Cruzeiro L. 2008. Influence of the sign of the coupling on the temperature dependence of
optical properties of one dimensional exciton models. Journal of Physics. B: Atomic, Molecular
and Optical Physics 41: 195401. IF=2.089.
3. Cruzeiro L. 2009. The Davydov/Scott model for energy storage and transport in proteins.
Journal of Biological Physics 35: 43-55. IF=0.506.
proceedings)
proceedings)
A provisional patent request has been made to INPI (Pedido Provisório de Patente, nº 104302 K).
The Biophysics group has an on-going collaboration with the Nonlinear Physics Group of the
University of Seville (Seville, Spain) and in particular with Prof. Francisco Romero (the leader)
and Dr. Jesús Cuevas Maraver, on the general theme of protein folding and function. In
particular we have been studying quantum thermalization schemes based on Lindblad
operators.
77
Leonor Cruzeiro is also an honorary research fellow at the Mathematics Department of HeriotWatt University, Edinburgh, Scotland, UK, where the Biophysics group collaborates with Prof.
Chris Eilbeck on the interaction of quantum particles with mechanical vibrations, with models
that are an extension of those applied to energy transfer in proteins.
The Biophysics group has also collaborated with Profs. Larissa Brizhik and Alexander Eremko,
from the Bogolyubov Institute for Theoretical Physics (Kiev, Ukraine) on the influence of
electromagnetic radiation on energy transfer in proteins, using a model developed by Prof. AS
Davydov, who was a leader there (now deceased).
A new collaboration was started in 2008 with Prof. Manuel Garcia Velarde of the Instituto
Pluridisciplinar of the Universidad Complutense de Madrid to study the influence of intrinsic
nonlinearity of the lattice on the electron states. This collaboration has so far involved two visits
by L Cancela to Prof. M Velarde’s Institute and a visit by Dr. Oliva Cantu Ros, a postdoc with
Prof. MG Velarde, to CCMAR, to work with L Cancela.
8e. Future research
1. Objectives
The objective of the Biophysics Group in the near future is to develop and consolidate the five
lines of research described in item Objectives and Achievements and to apply for patents
whenever appropriate. In 2009, the provisional patent request nº 104302 K, at INPI, will be
turned into a full patent application.
Concerning research line 1 the method developed to calculate the temperature dependence of
absorption spectra will be applied to the amide I band of alpha-helices and beta-sheets, as well
as to the organic crystal of acetanilide. Dr. Holly Freedman, who joined the Biophysics group in
April 2009, will be involved in this research.
Concerning line 2 the next aim is to perform further simulations of energy transfer from the
hydration waters to proteins with a varying number of glutamine residues in order to try to
understand why huntingtins with more than 36 successive glutamines are particularly
pathogenic. Project PTDC/SAU-GMG/098274/2008 (item Future research – pending funding), in
collaboration with Patrícia Faísca of CFTC, Lisbon, where other, more classical approaches to
misfolding diseases are pursued, is also related to this theme.
Concerning line 3, the collaboration with the Nonlinear Physics Group of the University of Seville
and with Prof. Chris Eilbeck has also been extended to Prof. Ana Nunes (CFTC Lisbon), who is the
PI in the Project PTDC/FIS/100276/2008 (item Future research – pending funding). One specific
aim is to determine operators (Lindblad operators) capable of establishing the coupling of the
amide I excitation to a thermal bath, within a full quantum mechanical framework.
78
Concerning line 4, the aim is to perform further simulations to characterize better the free
energy landscape of proteins. This requires long MD simulations for which a cluster with ca 64
cores is necessary. Project PTDC/BIA-PRO/098335/2008 (item Future research – pending
funding), in collaboration with Prof. Pedro Medeiros and MSc Paulo Afonso Lopes, both
computer scientists from CITI, UNL (Lisbon), and in collaboration with Dr. Paulo Martel, a
theoretical biochemist at CBME (Univ. Algarve), includes funds for such a cluster. The main aim
is to determine whether proteins can have structures that are very different from the native
ones and that are equally stable from a thermodynamic point of view.
2.

Current funding
ca 23.340 EUR/year. Postdoctoral grant for Dr. Holly Freedman, funded by FCT, ref.
SFRH/BPD/41143/2007.

Pending funding (3 applications in evaluation)
198.003 EUR, FCT, PTDC/BIA-PRO/098335/2008, Do protein native structures correspond to the
global minima of the free energy?.PI: L Cruzeiro.
101.302 EUR, FCT, PTDC/FIS/100276/2008 Physics of Biological Systems. PI: Ana Nunes (CFTC,
Lisbon), L Cruzeiro as collaborator.
92.260 EUR, FCT, PTDC/SAU-GMG/098274/2008, Intermediates states: At the crossroads of
pathways to folding and pathways to disease. PI: Patrícia Faísca, L. Cruzeiro as collaborator.
79
Biotechnology and Molecular Biology of Microalgae (BMBM)
Biotechnology and Molecular Biology of Microalgae (BMBM)
João Carlos Serafim Varela
13.428 EUR, Agência de Inovação SA, POCI 2010-IDEIA, BOTRYOFUEL, 13-05-04-FDR-00041,
Hydrocarbon hyperproducing strains, 2007-2008. Team member.
Agência de Inovação SA, PRIME-DEMTEC, BIOSOLAR, 70/00219, 2007-Present. Consulter.
IAPMEI, SIME, NEOPBR 00/21924, New discardable photobioreactor for sensitive microalgae,
2007-Present. Consulter.
6.000 EUR, Agência de Inovação SA, NITEC-GENTEC 161/26/06, Stimulating systems for the
creation of research & technological development nuclei in entrepreneurial sector, 2007Present. Team member.
4.1200 EUR, INTERREG IIIB - Atlantic Area, 159 SAL, SAL - Salinas do Atlântico. Recovery of the
identity of Atlantic salt pans (salinas). Recovery and promotion of the biological, economical,
and cultural potential of humid coastal areas, 2005-2008. Team Member.
1. Objectives
The Biotechnology and Molecular Biology of Microalgae (BMBM) group has focused its research
on the elucidation of the molecular basis of the massive carotenoid accumulation in the
microalga Dunaliella salina. This accumulation is triggered by abiotic stress such as high light
intensity, high salinity and / or nutrient depletion, and thus an integrative approach was chosen
in which the concomitant molecular response to stress has been studied as well. Recently other
research lines have been initiated. They are a natural progression from our focus on carotenoid
biosynthesis. As carotenoids are soluble in lipids, our team has researched novel methodologies
concerning lipid detection in microalgae by means of Fluorescent-Activated Cell Sorting (FACS).
80
In this research line we have isolated microalgal carotenoid hyperproducers by screening for
microalgae with high lipid content. Using this technique we are currently screening for lipid
hyperproducers for biodiesel production. Our current interests also include the screening,
isolation and identification of microalgae containing bioactive compounds with antioxidant, antiinflammatory, antiviral, anti-neurodegenerative and anti-tumor activities.
In the year of 2008, we published several peer-reviewed articles describing key genes involved
in the biosynthesis of carotenoids in microalgae and their precursors (isoprenoids). We have
established that Dunaliella salina seems to have a fully functional carotenoid biosynthetic
pathway that is fed by the MEP (methylerythritol phosphate) isoprenoid biosynthetic pathway.
Molecular data indicated that the genes of both pathways are nuclear and encode proteins that
are translocated into the chloroplast at a later stage. Most genes of both pathways are induced
by carotenogenic abiotic stress. Under conditions of “cell shading,” found in highly concentrated
microalgal cultures, nutrient depletion is the most important factor in the induction of
carotenogenesis and the pathways involved therein. If nutrients are present in D. salina
cultures, massive accumulation of carotenoids is inhibited. However, if “cell shading” is
minimized by decreasing cell volumes, the length of the light path and / or cell density, light
intensity becomes the most important carotenogenic factor, although other stresses may
further enhance carotenoid accumulation in the chloroplast of the microalga.
Concomitantly, we have developed a research line concerning the establishment of highthroughput techniques for the isolation of lipid hyperproducers via Fluorescent-Activated Cell
Sorting (FACS) adapted to microalgae. We have showed that FACS flow cytometry is an ideal,
highly sensitive technique for this purpose. Recently, using this method, we have found out that
high salinity induces the production of total lipids in a microalgal strain isolated by our
entrepreneurial partner (Necton, S.A.). Currently, we are investigating the lipid classes affected
by salinity. As this algal strain doubles in cell density in less than 24 hours, this microalga seems
to be a primary candidate for local biodiesel production. Viability studies have been carried out
indicating that this alga could compete with fossil-derived diesel if oil prices are higher than 55
USD / barrel. At the present time, the NYMEX Crude Future, the Dated Brent Spot and WTI
Cushing Spot indexes are between 57 and 58 USD / barrel (Bloomberg, 2009, available at
http://www.bloomberg.com/energy/ [accessed on May 18, 2009]).
8d. Productivity
1. Ramos, A, Varela, J. 2008. Biochemistry and molecular biology in Portugal: an overview of past
and current contributions. IUBMB Life 60: 265-269. IF=2.364.
2. Ramos, A., Coesel, S, Marques, A, Rodrigues, M, Baumgartner, A, Noronha, J, Rauter, A,
Brenig, B, Varela, J. 2008. Isolation and characterization of a gene encoding a functional
lycopenestress-inducible Dunaliella salina Lcy- -cyclase. Applied Microbiology and
Biotechnology 79: 819-828. IF=2.569.
81
3. Mendoza, H, Freijanes, K, Carmona, L, Ramos, A, Duarte, V, Varela, J, de la Jara, A. 2008.
Characterization of Dunaliella salina strains by flow cytometry: a new technique to select
carotenoid hyperproducing strains. Electronic Journal of Biotechnology 11(4):
doi:10.2225/vol11-issue4-fulltext-2. IF=0.920.
602-611. IF=2.544.
5. Coesel, S, Oborník, M, Varela, J, Falciatore, A, Bowler, C. 2008. Evolutionary origins and
functions of the carotenoid biosynthetic pathway in marine diatoms. PloS ONE 3(8): e2896.
doi:10.1371/journal.pone.0002896.
6. Ramos, A, Marques, A, Rodrigues, M, Henriques, N, Baumgartner, A, Castilho, R, Brenig, B,
Varela, J. 2009. Molecular and functional characterization of a cDNA encoding 4-hydroxy-3methylbut-2-enyl diphosphate reductase from Dunaliella salina. Journal of Plant Physiology 166:
968-977. IF=2.437. (this article should have been published in 2008, but due to delays caused by
the enormous amount of articles in the Elsevier’s pipeline, it got published in 2009).
proceedings)
proceedings)
PhD THESES
Coesel, SN. 2008. Regulation of the carotenoid biosynthetic pathway in the green microalga
Dunaliella salina and the diatom Phaeodactylum tricornutum. PhD thesis, University of Algarve.
Supervisor: J Varela and C Bowler.
16th National Congress of Biochemistry, Ponta Delgada, Azores, 22-25 October 2008. This event
was organized by the Portuguese Biochemical Society (SPB). João Varela is a member of the SPB
Board of Directors.
82
Necton S.A. – Portuguese biotechnology company. Agreement for the development of novel
algal strains for biotechnological production of biodiesel from microalgal sources. This
agreement was a consequence of the BOTRYOFUEL project.
Our team collaborates with several research groups, which is displayed in joint publications in
our peer-reviewed publication track record. The groups we collaborate with include:
-
Stazione Zoologia, Napoli, Italy. Group Leader: Dr. Chris Bowler. We have collaborated in
the elucidation of how the carotenoid biosynthetic and the plastidial MEP
(methylerythritol phosphate) isoprenoid biosynthetic pathways interact and are
regulated in green microalgae and diatoms.
-
Institute of Veterinary Medicine, Goettingen, Germany. Group Leader: Prof. Dr. Bertram
Brenig. This collaboration was a part of a joint effort to develop pigment-rich animal
feeds from microalgal biomass.
-
Canary Technological Institute, Canary Islands, Spain. Group Leader: Dr. Héctor
Mendoza. This collaboration in resulted in a joint publication about the development of
novel techniques for high-throughput isolation of microalgal lipid hyperproducers.
-
Rutgers University, Piscataway, New Jersey, USA. Group Leader: Prof. Terri G. Kinzy. This
collaboration is part of an effort to elucidate the regulation and function of molecular
players that may participate in the stress response of microalgae under carotenogenic
conditions.
-
Brooklyn College, NY, USA. Group Leader: Dr. Juergen Polle. This collaboration is part of
the international effort in sequencing the genome of Dunaliella salina with the help of
the US Department of Energy.
-
University of Nevada, USA. Group Leader: Dr. John Cushman. This collaboration is part
of the international effort in sequencing the genome of Dunaliella salina with the help
of the US Department of Energy.
83
8e. Future research
1. Objectives
BMBM will continue the research of the molecular basis of the massive carotenoid accumulation
in Dunaliella salina cells under abiotic stress. In collaboration with John Cushman and Juergen
Polle (see Group productivity - Internationalization for details), BMBM will also participate in the
effort led by the US Department of Energy for the nucleotide sequencing of D. salina genome.
We have generated more than 8000 ESTs as a starting point for this effort. Some of these ESTs
have been extended by RACE PCR and the respective full-length cDNA clones have been
obtained. One of these has already resulted in a publication (Ramos, et al., 2009, J Plant Physiol
166, 968). Recently, by means of a yeast two-hybrid screen, we were able to isolate a putative
interactor that seems to bind to lycopene beta-cyclase, a key enzyme for the production of the
most abundant carotenoid in D. salina: beta-carotene. This protein is highly conserved in
photosynthetic organisms. Preliminary phylogenetic analysis revealed that it is present from
cyanobacteria to rice; however, its function is virtually unknown. In silico analysis of the
polypeptide indicates that this putative interactor belongs to the thioredoxin superfamily. We
will further research how this protein binds to lycopene beta-cyclase with pull-down assays and
the effect of this binding on enzyme activity and carotenoid biosynthesis. Interestingly, this
protein may be an important player in carotenogenesis regulation by the redox state of the cell
as postulated previously by Lamers et al. (2008) (Trends Biotechnol. 26, 631).
Concurrently we will continue our research towards the isolation and characterization of
microalgae for biodiesel production. For this purpose, BMBM will initiate a screening of local
waters for rapidly dividing microalgal cells with high lipid content. The initial screening will be
carried out by means of FACS, which will enable us to screen millions of cells in hours. These
cells will be subjected to different forms of abiotic stress known to stimulate lipid biosynthesis,
namely high light intensity, salt stress and / or nutrient depletion. We will finalize the
characterization of microalgal strains already cultivated by our entrepreneurial partner (Necton
S.A.) by means of Gas Chromatography - Mass Spectrometry (GC-MS) to determine the precise
lipid composition and estimate their potential for biodiesel and polyunsaturated fatty acid
production.
In 2009, we have initiated a third line of research comprising the screening of marine
photosynthetic organisms for bioactive compounds with antioxidant, anti-inflammatory,
antiviral, anti-neurodegenerative and anti-tumor activities. Collaboration with the former
director of the Parc Cientific de Barcelona, Fernando Albericio and Prof. Amélia Rauter (FCUL,
Lisbon) will enable us to isolate and characterize biomedically relevant novel compounds by
means of NMR, Infra-Red spectroscopy and ex vivo screening with mammalian cell lines. BMBM
will also join a European Glycomics Network, as recent data has shown the high potential of
glycosides for novel biomedical applications.
2.

Current funding
Agência de Inovação SA, PRIME - DEMTEC. BIOSOLAR - 70/00219, 2007-Present. Consulter.
84
IAPMEI, SIME, NEOPBR 00/21924, New discardable photobioreactor for sensitive microalgae,
2007-Present. Consulter.
6.000 EUR, Agência de Inovação SA, NITEC-GENTEC 161/26/06, Stimulating systems for the
creation of research & technological development nuclei in entrepreneurial sector, 2007Present. Team member.

Pending funding
42.5482 EUR, European Commission, FP7, KBBE-2009-3-2-02, BIOALGAE - Omega-3 fatty acid
production from marine microalgae, 2009.
11.7472 EUR, FCT, Enzymes and key regulators of stress-induced massive carotenoid
iosynthesis in Dunaliella salina, 2009.
2009, FCT PTDC/MAR/103957/2008. SEABIOMED - Marine Photosynthetic organisms of the
Algarve Coast with Biomedical Applications. BMBM budget: 159.002 EUR.
117.472 EUR, FCT, PTDC/BIA-MIC/101036/2008, CYANO - Iron Homeostasis regulation in
cyanobacteria, 2009.
85
BioVanadium
BioVanadium Research Group (only at CCMAR in 2006 and 2007)
Manuel Aureliano Pereira Martins Alves
1. Objectives
Vanadium is one of the important transition elements in biology. In aqueous solution vanadium
(V) occurs in a number of oxometalates called vanadates. At neutral pH and through the range
of concentrations most frequently employed in biochemical studies, the predominant vanadate
species are the monomer (H2VO4-), dimmer (H3V2O7-) and tetramer (V4O124-). These species
reach chemical equilibrium on a millisecond time scale making difficult the identification of the
oligomers responsible for the effects promoted in biological systems.
Myosin is a highly specialised protein involved in the process of muscle contraction, which along
with actin, converts the chemical energy of ATP hydrolysis to mechanical work. Although the
actomyosin ATPase activity has been described to be about 90% inhibited by V1, it was
suggested that this inhibition might be due in part to the presence of polymeric vanadate ions.
In fact, it has been suggested that decavanadate interacts with myosin, being responsible for the
vanadate-induced photolytic cleavage of myosin subfragment-1 (S1) although little is known
about its putative binding sites.
Sarcoplasmic reticulum (SR) Ca2+-ATPase is a transmembrane transport system, which
accumulates Ca2+ at expense of ATP splitting during the process of muscle relaxation. ATP is
used in a process involving the transfer of the phosphoryl group to the Ca2+-ATPase with
subsequent breakdown of the phosphorylated enzyme. It was demonstrated that some of the
interactions, e.g. decameric species, disrupt the energetic coupling and the enzyme turnover.
86
Other interactions of vanadium, e.g. monomeric species, may be without effect or even improve
the coupling of Ca2+ pumping.
Oxidative stress studies induced by and vanadium are also almost limited to hepatic and renal
injury studies. On other hand, the contribution of decavanadate to vanadium toxicity is usually
not considered. The different responses obtained on in vivo and in vitro studies proves that in
vivo metals metabolism is very complex and great care must be taken on extrapolation from in
vitro conditions. Recently, it was demonstrated that decameric vanadate species are responsible
for a strong increase on lipid peroxidation and a decrease in cytosolic catalase activity thus
contributing to oxidative stress responses upon vanadate intoxication.
1) Contraction namely in the contractile system (myosin and actin) and regulatory system
(calcium pump from sarcoplasmic reticulum; 2) Cellular responses induced by vanadium:
oxidative stress, lipid peroxidation, metal accumulation, mechanism of cell death, 3) Effects of
vanadium compounds drugs in type 2 diabetes; 4) Mechanisms of insulin resistance in diabetes
and 5) Effects of reactive nitrogen species in neurons.
8d. Productivity
Toxicology 24: 253-263. IF=2.155.
2. Soares, SS, Martins, H, Gutiérrez-Merino, C, Aureliano, M. 2008. Vanadium and cadmium in
vivo effects in teleost cardiac muscle: metal accumulation and oxidative stress markers.
Comparative Biochemistry and Physiology C 147: 168-178. IF=2.530.
3. Soares, SS, Henao, F, Aureliano, M, Gutiérrez-Merino, C. 2008. Vanadate induces necrotic cell
death in neonatal rat cardiomyocytes through mitochondrial membrane depolarization.
Chemical Research in Toxicology 21: 607-618. IF=3.491.
4. Tiago, DM, Laizé, V, Aureliano, M, Cancela, ML. 2008. Vanadate proliferative and anti
cell line. FEBS Letters 582: 1381-1385. IF=3.264.
5. Aureliano, M, Henao, F, Baruah, B, Crans, DC. 2008. Sarcoplasmic reticulum calcium ATPase is
inhibited
by
organic
vanadium
coordination
compounds:
pyridine-2,6dicarboxylatodioxovanadium(V), BMOV, and an amavadine analogue. Inorganic Chemistry 47:
5677-5684. IF=4.147.
87
proceedings)
1. Tiago, T, Aureliano, M, Gutiérrez-Merino, C. 2008. Effects of reactive oxygen and nitrogen
species on actomyosin and their implications for muscle contractility. In: Oxidative stress,
Research Signpost, Kerala, India. (in press).
proceedings)
PhD THESES
Tiago, DM. 2008. Role of insulin and insulin like peptides in bone formation: identification of
bone specific target genes and regulatory mechanisms, and characterization of the insulin
mimetic effect of vanadium. PhD thesis, University of Algarve. Co-supervisor: ML Cancela, V
Laizé and M Aureliano.
8e. Future research
1. Objectives
Effects of vanadium compounds drugs in type 2 diabetes; Mechanisms of insulin resistance in
diabetes and effects of reactive nitrogen species in neurons.
88
2.

Current funding
89
Eduardo Jorge Sousa Rocha
Funds spent in 2008:
-
-
National funding
8.473,62 EUR, FCT POCI/MAR/59462/2004, The molecular mechanisms of sexual differentiation
in gastropods and the role of vertebrate-like steroid hormones.
FCT PTDC/MAR/70436/2006, An integrative study on the toxicopathic lesions in Portuguese
estuarine fishes - Assessing injury impact and toxicogenomic implications in experimental
models.
14.726,13 EUR, FCT PTDC/MAR/68885/2006, Lipid metabolism disruption in marine fish by
xenobiotic ligands of peroxisome proliferator-activated receptors (PPARs).
1. Objectives
The strategic objectives set up in 2003 were to build a multidisciplinary group and to establish a
lab space at the CIIMAR “new” facilities in Porto. We aimed a team able to support
multidisciplinary studies and keen to establish cooperation with other national and international
groups. The research efforts at LECEM (now LECEMA) soon targeted both fundamental and
applied studies, correlating structure with function in selected fish and molluscs, either in
normal and pathological scenarios (namely in a toxicological context). Some emphasis was put
on influences of steroid hormones and xenobiotics in unexplored targets, from molecular (eg.
90
molecular mechanisms of imposex and of PPARs involvement in peroxisomal changes) to organ
levels (eg. liver responses to subacute toxicity).
As to non-experimental studies, our main objective has been to contribute for a better
knowledge of the structure and function of the fish liver, and other related aspects of the
digestive tract, considering gender and seasonal effects. More recently, those studies also
involved the kidney, establishing a comparative approach between peroxisomal events detected
in liver and that are explored as a new thesis of endocrine (estrogenic) modulation of
peroxisomal function. Comparative studies were also made in the digestive apparatus of
molluscs, including hydrothermal-vent bivalves from the Azores. These approaches aimed to
provide sound baseline data to support toxicopathological studies, which were initiated
combining biomarkers with analytical chemistry targeting toxicants.
As to experimental studies, they targeted two main biological problems: 1) how and to what
extent fish peroxisomes are regulated and disrupted by estrogenic compounds, and (recently)
by xenobiotic ligands of PPARs; and 2) what are the molecular mechanisms involved in the
development of male penis and female imposex. Both themes derive from concerns about
endocrine disruption in aquatic animals. In additional experiments, in cooperation with other
research groups at CIMAR and from other institutions, our group intervened implementing
histopathology for diagnosis of toxicophatic lesions and also testing new quantitative
approaches at tissue level for evaluating the intensity of effects of reference xenbiotics, like
pesticides. We aimed correlate those findings with chemical toxicology surveys for endocrine
disruptions in several estuaries.
Scientific output in publications
-
We published 10 international articles in peer-reviewed indexed journals, and co-edited
1 peer-reviewed book. Facing that in 2007 and in most of 2008 the number of PhDs in
the group was actually 5, we had a real mean production rate of about 2 peer-reviewed
publications / per PhD / per year. Additionally, the team attended both national and
particularly international meetings in physiology and toxicology, making over 15
presentations (including posters and platform talks).
Increasing scientific networking and collaborations
-
From our 11 international publications, we had 4 articles and 1 book in co-authorship
with colleagues abroad published (about 50% of our output), thus materializing our aim
to increase internationalization and maintain good international collaborations. Also,
cooperation with other Laboratories of the CIMAR LA and also with other national
colleagues occurred. A network of continuing interactions was thus established or
reinforced.
Support to advance training
-
Our efforts to support both Master and PhD Students resulted in the conclusion of 3
PhD theses in the evaluation period. We housed in 2008, and supervised, 6 PhD
students which continued their thesis works. Two Master students were accepted;
91
despite they will start the laboratory project in 2009. We reinforced the group with an
Auxiliary Researcher (coming from Germany). All PhD gave support to master and PhD
mentoring and/or advanced teaching in courses.
International recognition by publishing houses and editors
-
Team members have served as peer-reviewers in several indexed journals. The PI
continued to be the Editor-in-Chief of the journal Comparative Hepatology (BioMed
Central - Springer). In addition to projects concluded up to 2007, team members
concluded the leadership of 1 book project (released early in 2008): Rocha MJ, Arukwe
A, Kapoor BG. Fish Reproduction (Science Publishers). There were new contacts with the
PI towards making a chapter for a new book series: Ovary: Development and Functions.
In: Hormones and Reproduction in Vertebrates, Fishes (Academic Press).
8d. Productivity
Environmental Monitoring and Assessment 145: 315-322. IF=1.035.
2. Jordanova, M, Miteva, N, Rocha, E. 2008. A qualitative and quantitative study of the hepatic
pigmented macrophage aggregates during the breeding cycle of Ohrid trout, Salmo letnica Kar.
(Teloestei, Salmonidae). Microscopy Research & Technique 71: 822-830. IF=1.883.
3. Kadar, E, Davis, SA, Lobo-da-Cunha, A. 2008. Cytoenzymatic investigation of intracellular
digestion in the symbiont-bearing hydrothermal bivalve Bathymodiolus azoricus. Marine Biology
153: 995-1004. IF=1.953.
4. Kortner, TM, Rocha, E, Silva, P, Jørstad, TS, Castro, LFC, Arukwe, A. 2008. Genomic approach in
evaluating the role of androgens on the growth of Atlantic cod (Gadus morhua) previtellogenic
oocytes. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 3: 205–
218. IF=1.543.
5. Lobo-da-Cunha, A, Calado, G. 2008. Histology and ultrastructure of the salivary glands in Bulla
striata (Mollusca, Opisthobranchia). Invertebrate Biology 127: 33-44. IF=1.217.
6. Luís, A, Rocha, E, Oliveira, E, Silva, J, Barros, A, Sousa, M. 2008. Stereological analysis of
mitochondria and smooth endoplasmic reticulum distribution in oocytes in prophase I.
Microscopy and Microanalysis 14(S3): 103-104. IF=2.992.
7. Monteiro, SM, Rocha, E, Fontaínhas-Fernandes, A, Sousa, M. 2008. Quantitative
histopathology of Oreochromis niloticus gills after copper exposure. Journal of Fish Biology 73:
1376-1392. IF=1.246.
92
8. Monteiro, SM, Rocha, E, Mancera, JM, Fontaínhas-Fernandes, A, Sousa, M. 2008. A
stereological study of copper toxicity in gills of Oreochromis niloticus. Ecotoxicology and
Environmental Safety 72:213-23. IF=2.590.
9. Santos, MM, Reis-Henriques, MA, Guillot, R, Lima, D, Franco-Duarte, R, Mendes, I, Queiros, S,
Castro, LFC. 2008. Anti-androgenic effects of sewage treatment plant effluents in the
prosobranch gastropod Nucella lapillus. Comparative Biochemistry and Physiology C –
Toxicology & Pharmacology 148: 87-93. IF=2.530.
differentiation and growth in blackspot seabream (Pagellus bogaraveo, Brunnich): histochemical
and immunohistochemical study of the fibre types. Tissue & Cell 40: 447-458. IF=0.738.
Note: Additional papers were accepted for publication in 2008, some even appeared online 1st
still in 2008, but they were published in the final printed format only in 2009, and so they will be
listed only in the 2009 report.
proceedings)
1. Rocha, MJ, Arukwe, A, Kapoor, BG. 2008. Fish Reproduction. Science Publishers Inc., Enfield,
New Hampshire, USA, 629 pp. ISBN 978-1-57808-331-2.
proceedings)
PhD THESES
Pinto, CB. 2008. Peroxissomas in brown trout (Salmo trutta fario): regulation by estrogens. PhD
thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisors: A Lobo da Cunha and E
Rocha, P Rodrigues (IBMC / INEB LA, ICBAS).
Resende, AD. 2008. Seasonal and toxicological study of brown trout (Salmo trutta) kidney and
liver peroxisomes. PhD thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisors: A
Lobo da Cunha and E Rocha.
93
Collaborative projects continued in 2008, with groups in Macedonia (several partners), Japan
(several partners), Norway (NTNU, Trondheim), UK (King’s Colleague and CEFAS), USA (Duke
University), and Thailand (Kasetsart University, Bangkok). Examples of collaborative publications
are:
1. Kortner, TM, Rocha, E, Silva, P, et al. 2008. Genomic approach in evaluating the role of
androgens on the growth of Atlantic cod (Gadus morhua) previtellogenic oocytes. Comparative
Biochemistry Physiology D: Genomics and Proteomics 3: 205-218. Partner: NTNU, Norway.
2. Kortner, TM, Rocha, E, Arukwe, A. 2008. Androgenic modulation of early growth of Atlantic
cod (Gadus morhua L.) previtellogenic oocytes and zona radiata-related genes. Journal of
Toxicology and Environmental Health (accepted in 2008). Partner: NTNU, Norway.
3. Silva, P, Rowlerson, AM, Valente, LMP et al. 2008. Muscle differentiation and growth in
blackspot seabream (Pagellus bogaraveo, Brunnich): histochemical and immunohistochemical
study of the fibre types. Tissue & Cell (accepted in 2008). Partner: King’s College, Univ. London,
UK.
(Teloestei, Salmonidae) Microscopy Research and Technique (accepted in 2008). Partner: Univ.
Sts. Cyril & Methodius, Macedonia.
The PI was invited to integrate his team in a European network project named Biological
Effects Quality Assurance in Monitoring Programmes (BEQUALM). Also, the PI is helping
inserting CIMAR LA in AQUA-TNET (Socrates-Erasmus Thematic Network in Aquaculture,
Fisheries and Aquatic Resource Management). This will allow networking the Master in Marine
Sciences and Resources (Univ. Porto), in which the team participates. The PI is a Deputy Director
of the new inter-university PhD Program in Marine and Environmental Sciences, and is
cooperating in launching a new Doctoral Program with the University of Galicia (Spain), upon
request of the Porto and alicia authorities and the Univ. Porto Rector. The PI’s roup provides
advanced training/classes in those Master and PhD programs. The Group housed PhD students
from Brazil (1) and Norway (1).
94
8e. Future research
1. Objectives
The strategic goals defined by the PI for the next 3 years are to: 1) increase in 10% the articles
with international partners and the articles average impact; 2) increase in 10% the number of
articles/PhD/year; 4) conclude 2 theses/year; 5) focus on 2 research lines (see below); and 6)
run an annual course on cell and molecular biology techniques.
Line 1) Fish peroxisomes and related nuclear receptors (PPARs, RXRs, RARs); interplay
mechanisms, endocrine modulation, and metabolic consequences of disruption
-
We will continue to publish data derived from past efforts, proving that fish
peroxisomes are modulated by sex steroids, particularly in liver and by estrogenic
stimuli, apparently involving PPARs. Also, we will study interactions between
peroxisomal / PPARs activating vs repressing (estrogens) ligands. Work will be
conducted with the so-called peroxisome proliferators (PPs). Some of them have been
detected worldwide, and concern about their use is growing due to their persistence,
toxicity and bioaccumulation. We will explore the hypothesis that those exogenous
PPARs ligands may disrupt lipid metabolism in fish, affecting health, survival, growth
and, lipid/FA profiles. A gene array approach is integrated. We will contribute with new
insights to understand lipid and energy metabolism control in fish, with possible
application on risk assessment and aquaculture. In parallel, it will be also described the
action of PPs on other genes not involved in lipid metabolism and possibly not under
PPARs control, as the result of the use of advanced molecular tools. As it known that
PPARs cross-talk with RXRs, we are co-responsible for project that involves a
multiparametric approach, combining the study of variations at the gene and protein
(enzymes) levels of key disruption prone targets, but also changes in sexual
development and reproduction. We expect to identify relevant metabolic pathways
prone to disruption by RARs, RXRs, PPARs agonists.
Line 2) Toxicopathology, Toxicogenomics and Chemical Toxicology
-
We launched a multidisciplinary project, integrating transcriptomics, which will set
grounds for an expansion and cooperative efforts into future proteomics and
metabolomics. The project background is the fact that toxicophatic injuries in estuarine
fish are increasing. Histopathology often reveals liver lesions and intersex condition.
These situations may translate either acute and/or chronic chemical stress. Field surveys
targeting fish toxicopathic lesions are being made in Europe and USA, in parallel with
the use of other biomarkers and chemical analyses. Pathology surveys are very scarce in
Portugal and so we aim to lead them. The new project is unique in making a 1st
systematic survey of toxicopathic lesions in Portuguese estuarine fish, whereas
experimentally tackling effects of lesions at diverse biological levels. Some of the aims
are to: 1) document toxicopathic fish lesions in estuaries in the north and centre; 2)
clarify in what extent the lesion type and severity relates with pollution; 3) know better
fish carcinogenesis and find ways to early detect related lesions; 4) clarify if/in what
extent hepatic necrosis and neoplasia interferes with general liver function and
detoxification, growth/survival; 5) conclude about gender susceptibility and implications
95
of fish neoplasia; 6) study gene signatures of tumour liver progression in a fish model; 7)
develop a focused cDNA array to support experimental modeling. We will generate field
data and also mechanistic insights.
2.

Current funding
38.500,00 EUR, FCT Pluriannual funding.
184.929,00 EUR, FCT PTDC/MAR/68885/2006, Lipid metabolism disruption in marine fish by
xenobiotic ligands of peroxisome proliferator-activated receptors (PPARs). July 2008-June 2011.
187.028,00 EUR, FCT PTDC/MAR/70436/2006. An integrative study on the toxicopathic lesions in
Portuguese estuarine fishes – Assessing injury impact and toxicogenomic implications in
experimental models. Oct 2008-Sept 2011.
71.164,00 EUR, FCT PTDC/MAR/68106/2006, The modulation of retinoic acid signalling
pathways by environmental pollutants in teleosts. Sept 2008-August 2011. Total Funding:
188.980,00 EUR, Project in cooperation with the Ecotoxicology, Genomics and Evolution Group
and the Environmental Toxicology Group of CIMAR.

Pending funding (under evaluation)
FCT PTDC/AAC-AMB/104970/2008, ANDRO-DOURO: Endocrine disruption by androgen receptor
antagonists - First chemical survey in a Portuguese River and new insights on their impact on the
reproductive biology of fish.
FCT PTDC/MAR/100184/2008, New advances in the morphofunctional comprehension of the
digestive system of carnivorous and herbivorous opisthobranch molluscs.
FCT PTDC/MAR/105199/2008, The invertebrate repertoire of nuclear receptors: evolutionary
and endocrine disruption insights.
96
Maria Teresa Sa Dias de Vasconcelos
In the year 2008 the CS&B Lab was involved in four financed projects/protocols at the National
level and one at the International level, either as a coordinator (in four projects/protocols) or as
a collaborator (in one projects/protocols). Two of those projects were financed by private
companies (Amorim Revestimentos S.A. and Petróleos de Portugal S.A.-Refinaria do Porto)
whereas the other three were financed by FCT (projects POCTI/CTA/48386/2002,
POCI/AMB/60267/2004 and ERA-AMPERA/0003/2007; this last one was also financed by the
Spanish partner). Another source of financing was the Plurianual FCT funds attributer to PhD
researchers. Total funds involved in the mentioned period was ca. 100 000 EUR. A majority of
those funds (ca. 45%) were obtained through contract-research activities (in cooperation with
private companies), whereas research projects financed by FCT and project partner accounted
for ca. 39% and Plurianual FCT funds represented ca. 16% of the total funds.
1. Objectives
Research carried out at CS&B Lab intends to give a contribution for the comprehensiveness of
biogeochemical processes in aquatic environment and soil in order to be able to find
remediation solutions when required. It may be also relevant for risk assessment purposes.
Main objectives for 2008 were as follows:
-
To prosecute the studies on mutual interactions among phytoplankton (mainly
cyanobacteria) and trace metals or emerging contaminants (endocrine disrupters and
antibiotics).
-
To prosecute the studies on the application of vascular plants for rhizoremediation,
giving emphasis on the plant role in conditioning the nearby environment. This included
97
the study of the effects of either inorganic and organic priority pollutants, as well as
possible antagonisms or synergisms caused by the concomitant presence of pollutants
of different families. On this topic, a new line of research was launched: investigation of
suitable biological remediation processes for the reduction/elimination of residues of
very much used pharmaceuticals compounds which are released from waste water
treatment plants (WWTP) into the aquatic environment.
-
To launch a new line of research on bioremediation of beaches contaminated with
petroleum hydrocarbons.
-
To prosecute the research on metal pollution and biological effects on fishes and to
extent it to organic pollutants, namely PAHs.
These studies included financed projects in progress, collaboration protocols, PosDoc, PhD and
MSc projects and the start of new projects.
Studies on mutual interactions of phytoplankton and compounds with endocrine disrupting
effects and pharmaceutics prosecuted anchored by few projects (under-graduation, 1 PhD and 1
Pos Doc). One paper was published and another accepted. The line focused on mutual influence
of metallic species and cyanobacteria resulted on a concluded PhD and 1 paper submitted.
Collaboration with LEAN in a PhD project on freshwater cyanobacteria alelopatic properties
resulted in 2 submitted papers.
Studies regarding optimization of analytical methods for determination of pharmaceuticals were
initiated in a new PhD project.
Toxicity studies of Esmoriz/Paramos coastal lagoon sediments and fish prosecuted focused on
organic pollutants. Water quality of aquaculture enterprises monitoring went on within a
collaboration protocol.
Studies on the role of vascular plants in conditioning the nearby environment and plant
capability for rhizoremediation also prosecuted (1 financed project, 1 concluded PhD (in
collaboration with Algarve University) and 3 concluded MSc). Four articles were published, 2
accepted, 4 submitted/in preparation. Investigation of the influence of compounds released by
vascular plants into the medium (exudates) was intensified. Optimization of methodologies for
detection and quantification of these substances in water samples were carried out in a PhD
project.
Suitability of biological processes for remediation of soil contaminated with petroleum
hydrocarbons (PHC) prosecuted (PhD in progress) focused in optimization of expeditious
methods to estimate degradation extent. A paper is in preparation. A new line of research was
initiated in collaboration with CIIMAR Hydrobiology Lab (an international interdisciplinary
project) concerning PHC bioremediation in contaminated beaches, and involves 3 fellowship
students.
Studies on indoor air quality continued with 1 financed project, 1 collaboration protocol and 2
MSc theses (1 finished, 1 in progress). An article was published.
98
Collaboration with several small and medium companies and centres prosecuted resulting in
projects and training courses for under-graduate and graduate students. Three MSc theses were
also concluded.
Results of the different studies were spread in 8 national and international conferences, as oral
or poster presentations.
8d. Productivity
1. Almeida, CMR, Mucha, AP, Bordalo, AA, Vasconcelos, MTSD. 2008. Influence of a salt marsh
plant (Halimione portulacoides) on the concentrations and potential mobility of metals in
sediments. Science of the Total Environment 403: 188-195. IF=2.579.
2. Almeida, CMR, Mucha, AP, Delgado, MFC, Caçador, IM, Bordalo, AA, Vasconcelos, MTSD.
2008. Can PAHs influence Cu accumulation by salt marsh plants? Marine Environmental
Research 66: 311-318. IF=2.032.
3. Azenha, MA, Evangelista, R, Martel, F, Vasconcelos, MTSD. 2008. Estimate of the digestibility,
assimilability and intestinal permeability of butyltins occurring in wine. Food and Chemical
Toxicology 46: 767-773. IF=2.321.
Pollution 151: 408-413. IF=3.135.
5. Buszewski, B, Ligor, T, Filipiak, W, Vasconcelos, MTSD, Pompe, M, Veber, M. 2008. Study of
sorptive properties of trap systems for selective enrichment of volatile organic compounds from
tobacco smoke samples. Toxicological and Environmental Chemistry 90: 51-64.
6. Carvalho, PN, Rodrigues, PNR, Alves, F, Evangelista, R, Basto, MCP, Vasconcelos, MTSD. 2008.
An expeditious method for the determination of organochlorine pesticides residues in estuarine
sediments using microwave assisted pre-extraction and automated headspace solid-phase
microextraction coupled to gas chromatography-mass spectrometry. Talanta 76: 1124-1129.
IF=3.206.
Environmental Monitoring and Assessment 145: 315-322. IF=1.035.
8. Fernandes, C, Fontaínhas-Fernandes, A, Ferreira, M, Salgado, MA. 2008. Oxidative stress
response in gill and liver of Liza saliens, from the Esmoriz-Paramos coastal lagoon, Portugal.
Archives of Environmental Contamination and Toxicology 55(2): 262-269. IF=1.864.
99
9. Fernandes, C, Fontaínhas-Fernandes, A, Cabral, D, Salgado, MA. 2008. Heavy metals in water,
sediment and tissues of Liza saliens from Esmoriz-Paramos lagoon, Portugal. Environmental
Monitoring and Assessment 136: 267-275. IF=1.035.
maritimus and Scirpus maritimus) as sources of strong complexing ligands. Estuarine, Coastal
and Shelf Science 77(1): 104-112. IF=2.072.
IF=3.135.
IF=0.659.
13. Reis, PA, Almeida, CMR. 2008. Matrix importance in animal material pre-treatment for metal
determination. Food Chemistry 107(3): 1294-1299. IF=2.696.
14. Reis, PA, Valente, LMP, Almeida, CMR. 2008. A fast and simple methodology for
determination of yttrium as an inert marker in digestibility studies. Food Chemistry 108(3):
1094-1098. IF=2.696.
15. Stoichev, T, Baptista, MS, Basto, MCP, Carvalho, PN, Vasconcelos, MTSD. 2008. Application
of SPME to the determination of alkylphenols and bisphenol A in cyanobacteria culture media.
Analytical and Bioanalytical Chemistry 391(1): 425-432. IF=3.328.
proceedings)
Books (Chapter)
1. Fernandes, C., Fontaínhas-Fernandes, Salgado, MA. 2008. Metabolic mechanisms of
adaptation to chronic metal exposure - a case study. In: Heavy Metal Pollution. Brown, SE,
Welton, WC (Eds). Nova Science Publishers, ISBN: 978-1-60456-899-8.
Conference Proceedings
2. Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, A, Vasconcelos, MTSD. 2008. Influence of
organic pollutants on the phytoextraction potential of Halimione portulacoides. Proceedings of
the 3rd International Meeting on Environmental Biotechnology and Engineering (3IMEBE),
Palma de Maiorca, Spain, 21 - 25 September 2008.
proceedings)
100
MASTER THESES
Dias, AC. 2008. Influência de diferentes poluentes orgânicos na acumulação de cobre por uma
planta de sapal. Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor:
MTSD Vasconcelos.
Carvalho, P. 2008. Research on ex-situ remediation by Halimione portulacoides of contaminated
estuarine sediments with butyltin compounds. Master thesis in Chemistry, Faculty of Sciences,
University of Porto. Supervisor: MCP Basto.
Rodrigues, PN. 2008. Quantificação de poluentes orgânicos persistentes (POPs) organoclorados
em sedimentos e investigação ex-situ do papel da planta Halimione portulacoides na respectiva
bioremediação. Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor:
MTSD Vasconcelos.
Martins, S. 2008. Characterization of tobacco smoke produced/commercialized in Portugal.
Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor: MTSD
Vasconcelos.
Garcia, E. 2008. Gestão de solventes na indústria de fabrico e impressão de embalagens
metálicas. Master thesis in Environmental Sciences and Technology, Faculty of Sciences,
University of Porto. Supervisor: MTSD Vasconcelos.
Pozdniakova, T. 2008. Ecoeficiência empresarial e desenvolvimento de ferramentas de análise.
Master thesis in Environmental Sciences and Technology, Faculty of Sciences, University of
Porto. Supervisor: MTSD Vasconcelos.
Guimarães, ALG. 2008. Elaboração de uma ferramenta de ajuda à tomada de decisão no âmbito
do Regulamento de Sistemas Energéticos de Climatização de Edifícios no que concerne à
apreciação das metodologias de avaliação da Qualidade do Ar Interior. Master thesis in
Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor:
MTSD Vasconcelos.
PhD THESES
Silva, M. 2008. Metals and butyltins in sediments of Ria Formosa – The role of Spartina maritima
and Sarcocornia fruticosa. PhD thesis in Environmental Sciences and Technology, Faculty of
Sciences, University of Porto. Supervisor: MTSD Vasconcelos, Co-supervisor: M Caetano.
Baptista, M. 2008. Chemical factors related with freshwater blooms of cyanobacteria. PhD thesis
in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor:
MTSD Vasconcelos.
Santos, R. 2008. Optimization of methods of determination of isotopic ratios by icp-ms with
quadrupole and application to geological and environmental studies. PhD thesis in Chemistry,
Faculty of Sciences, University of Porto. Supervisor: MTSD Vasconcelos, Co-supervisor: Colombo
Tassinari.
101
Cooperation with:
-
Amorim Revestimentos S.A. and IDMEC on Indoor Air Quality.
-
Portuguese Association of Small and Media Companies on Environmental Diagnostics in
the Industry.
-
UNIMED on Chemical Characterization of Occupational Environmental.
-
Real Companhia Velha on Characterization of Port Wine.
-
SMGP - Consultores, Lda on Chemical Characterization of Occupational Environment.
-
Petróleos de Portugal S.A. Refinaria do Porto, GALP Energy Group - Petróleos de
Portugal S.A on Rizoremediação de Hidrocarbonatos de Petróleo em Solos da Refinaria
do Porto.
-
Paracélsia Industria Farmacêutica S.A. on Aluminum Determination on Serum Samples.
-
Hovione FarmaCiencia S.A. on Survey of Some Pharmaceutics Effects on Freshwater
Cyanobacteria. Does Good Manufacturing Practice (GMP) During Pharmaceutics
Manufacture Influence the Biological Response?
-
Department of Production and Systems, School of Engineering, University of Minho.
-
Small and Media Companies, like Sondar Company and ECOINSIDE - Soluções em
Ecoeficiência e Sustentabilidade, Lda., and Centres, like Centro de Apoio Tecnologia à
Indústria Metalomecânica (CATIM), for training courses and projects for under-graduate
and graduate students, namely for development of MSc thesis.
Collaborative publications
-
Buszewski, B, Ligor, T, Filipiak, W, Vasconcelos, MTSD, Pompe, M, Veber, M. 2008.
Toxicological and Environmental Chemistry 90(1): 51-64.
Socrates/Erasmus Bilateral Agreements in Environmental Chemistry (MTSD Vasconcelos)
102
-
Swiss Federal Institute of Technology, K. Hungerbuehier.
-
SECETOX, Masaryk University, Brno, Czech Republic, Ivan Holoubek.
-
Dep Environmental Chemistry and Ecoanalytics, Nicolas Copernicus University, Torun,
Poland, Boguslaw Buszewski.
-
Lab of Physic-Toxic-Chemistry of Natural Systems, Bordeaux University, Bordeaux,
France, Philippe Garrigues.
-
Environmental Analysis Group, National and Kapodistrian Athens University, Greece,
Panayotis Siskos.
-
Biology Faculty, Environmental Sciences and Biochemistry, Barcelona University, Spain,
Anna Riera.
-
Universidad del País Vasco - Bilbau, Spain.
-
Universidad Politecnica de Valencia, Spain.
Cooperation in International Organizations (MTSD Vasconcelos)
-
Member of the Editorial Board of: ESPR, Springer, Chemistry Central Journal.
-
Portuguese Chemical Society Delegate in "Division for Chemistry and the Environment
(DCE)" of the Federation of the European Chemical Societies, presently European
Chemistry and Molecular Science (EuCheMS).
-
International programs of Quality Control: Lead in Blood. Instituto Nacional de
Seguridad e Higiene en el Trabajo (INSHT), Zaragoza; Metals in Air. INSHT, Barcelona;
COVs in indoor air. Berufsgenossenschaftliches Institut fürArbeitsschutz – Round Robin
Test “Organic substances with thermodesorption”.
Review of scientific papers
-
Senior CS&B members act very frequently as reviewers for several international
scientific journals.
103
8e. Future research
1. Objectives
CS&B Lab will prosecute its lines of research in progress (some of which were launched very
recently) pursuing the final goal of gathering as much information as possible on mutual
interaction between organisms and the medium they are living in. Several areas previously
identified as strategic priorities for consolidation and growth of the group are already supported
by projects in progress, whereas others will be initiated.
Studies on mutual interactions of phytoplankton and compounds with endocrine disrupting
effects and pharmaceutics will prosecute under a Pos Doc program in progress and a new PhD
to star. Collaboration with LEAN, from CIIMAR, will provide different phytoplanktonic species
and with Hovione FarmaCiencia S.A. (a pharmaceutical Company) will provide required
chemicals, in addition to relevant specific knowhow. A recently started Pos Doc project on
cyanobacteria toxicins, carried out in collaboration with LEAN, also fits this research line.
Optimization of analytical methods for determination of pharmaceuticals will prosecute, being
extended to soils/sediments. Such analytical tools are required to search for suitable biological
processes for reduction/elimination of residues of those compounds from waters released from
WWTPs. In addition to the PhD in progress, a Pos Doc program will start at the beginning of
2009 to develop studies on this topic. A company devoted to construction and management of
water and WWTP will give the required technical support (a proposal of a project in cooperation
was submitted).
Studies on effects of Esmoriz/Paramos coastal lagoon pollution on fish will prosecute focused on
organic compounds, namely PAHs. Water quality of aquaculture enterprises monitoring will go
on within a collaboration protocol.
The role of vascular plants in conditioning the nearby environment and capability of such plants
for rhizoremediation of organic pollutants will prosecute. A new interdisciplinary (mainly
Chemistry and Biology) PhD project in collaboration with CIIMAR Hydrobiology Lab will be stared
in 2009. The study of the role of exudates on biogeochemical phenomena at the rhizosphere will
be intensified anchored in both 1 PhD in progress and 1 Pos Doc program which will start at the
beginning of 2009. Optimization of methodologies for isolation and pre-concentration are
crucial for speciation of exudates which are present at ultratrace levels in water and
soil/sediment matrixes. It is planned to include also some MSc programs in this topic.
The research to find suitable biological processes for PHC remediation in contaminated soil will
prosecute in the ambit of 1 PhD to be concluded in 2010. Related with this, bioremediation of
PHC in contaminated beaches is a new research line (collaboration with CIIMAR Hydrobiology
Lab in the ambit of an international interdisciplinary project) to be intensively developed. MSc
and under graduate students will be integrated in this project.
CS&B Lab will continue to render collaboration with small and medium companies and public
institutions in order to solve environmental problems which require CS&B Lab expertise.
Collaboration with public and private companies will be also useful for training of students, as
their first experience in the work market. In this ambit, at the end of 2008 three MSc theses are
104
in progress and new MSc and under-graduate training programs will be integrated in the next
future.
2.

Current funding
64.071 EUR, Rizoremediação de hidrocarbonetos de petr leo ...”. Cooperation protocol with
Petróleos de Portugal S.A., Refinaria do Porto. ADFCUP/CIIMAR. PI: Vasconcelos MTSD. Ends in
2009.
29.960 EUR, FCT - AMPERA Joint Management Committee (ERA-AMPERA/0003/2007),
OILDEBEACH - Buried oil in the intertidal beach zone: coupling between beach morphodynamic,
natural degradation, forcing mechanisms and biological activity, 2008-2011. 9960 EUR for a one
year scholarship and ca. 20000 EUR from Spanish partner for hydrocarbon analysis. Partners:
Univ. Vigo/GEOMA (Galicia/ES); CIIMAR (PT); Univ. Montpellier II/GLADYS (FR); ICBAS (PT).

FCT PTDC/AAC-AMB/100736/2008, GRINDOOR - Pollutants removal from indoor atmospheres
through phytoremediation by vascular plants. Participants: FEUP/IDMEC, CIIMAR and ICBAS.
FCT PTDC/MAR/099140/2008, PHYTOBIO - Phytoremediation and bioremediation of
contaminants in salt marshes: plant-microorganisms interactions. Participants: ICBAS and
CIIMAR.
FCT PTDC/AAC-AMB/103074/2008, REMOPHARED - Removal of emerging pollutants residues
from wastewater treatment plants effluents. Participants: FCUP, ICBAS, CIIMAR (CS&B - project
leader and Hydrobiology Labs) and CQO Plus company.
FCT PTDC/AAC-AMB/105134/2008 Parâmetros hematológicos e bioquímicos do sangue de
vertebrados aquáticos para aplicação na monitorização de poluição. Participants: CIM-IPB,
ICBAS-CIIMAR, UTAD.
105
Adelino Vicente Mendonca Canario
Refers only to funds spent in 2008 - approx. 340.000 EUR.
-
National public funds:
7.038 EUR, FCT PTDC/PSI/71811/2006, Ciclideo.
1.008 EUR, FCT POCI/BIA-BCM/60554/2004, Corn.
12.889 EUR, FCT POCI/CVT/55683/2004, Dourada.
18.889 EUR, FCT POCI/CVT/61052/2004, Péptidos.
16.979 EUR, FCT POCI/ PPCDT MAR/61091/2004.
105.245 EUR, FCT PTDC/CVT/66735/2006, PTHL.
17.986 EUR, FCT POCI/BIA-BDE/55463/2004, Tilapia.
181.800 EUR, Agência Ciência Viva, Project Latitude60! (science communication only).
-
Source: European Commission FP6
35.593 EUR, FP6 nº 044481 SSA, Aquagenome.
49.666 EUR, FP6 nº 012451, Finefish.
106
-
Source: Private funds
1.540 EUR, Caixa Geral de Depósitos (fellowship).
50.932 EUR - Services, bench fees, various sources.
1. Objectives
The main aim of the group is to study the role of hormones and their metabolites on the
physiology and the evolution of the endocrine system, using fish as the principal model and as
required other invertebrate and vertebrate models. The research is directed at four main
interrelated themes: 1) Hormonal control of Reproduction and Development; 2) Calcitropic
hormones, mineral metabolism and environmental adaptation, 3) Hormones, pheromones and
behaviour, 4) The impact of Genotype on phenotype. Specific objectives are:
Hormonal control of reproduction and development
-
To establish the identity, function and mechanisms of action of key regulatory factors in
relation to sex differentiation, puberty and gonad maturation.
-
To understand the process of metamorphosis and skeletal development in fishes, and
the role of thyroid and metabolic hormones.
Calcitropic hormones, mineral metabolism and environmental adaptation
-
To understand the evolution of mineral homeostasis and the role of endocrine
regulating factors, their site of action and the molecular mechanisms responsible for
their biological actions.
-
To understand the process of ossification and regeneration in fishes and the role of the
extracellular matrix, minerals and endocrine factors.
Hormones, pheromones and behaviour
-
To understand the underlying mechanisms regulating the interaction between
hormones and behaviour in fishes.
-
To understand how fish detect biologically important environmental chemical cues pheromones, food-related odorants and inorganic cations (Ca2+ and Na+) and how this
olfactory input is centrally processed to evoke the appropriate physiological and
behavioural responses.
107
The impact od genotype on phenotype
-
To apply an integrative and systems approach, from the genome to individuals and
populations, to the study of the evolution of endocrine hormones/systems and their
impact on phenotype.
Scientific discoveries:
-
Discovered that chemical signalling of dominance status occurs via controlled urination
in tilapia and that urinary odorants mediate male aggressiveness and female mate
choice.
-
Development and application of a non-invasive method to measure fish opercular
movements (gill ventilation rate) and assess autonomic responses of fish to relevant
chemical stimuli in the water.
-
Estimated the size of the olfactory epithelium transcriptome of goldfish/zebrafish and
its main components
-
Found that the N-terminal region of transthyretin explains differences in binding to
thyroid hormone between fish and mammals.
-
Integrated analysis of thyroid hormone system (thyroid morphology, hormones,
receptors, binding proteins and deiodinases) to resolve the developmental window
during which the thyroid hormone driven larval/juvenile transition occurs in a round
fish.
-
Characterisation of molecular changes underlying thyroid
responsiveness during metamorphosis in the Atlantic halibut.
hormone
tissue
Technology development /transfer:
-
Developed a sensitivity yeast assay to detect environmental estrogens based on
recombinant fish estrogen receptor beta.
-
Developed oligonucleotides microarrays for European sea bass and sea bream with Dr L
Bargelloni (Univ. Padova).
-
Organized comparative study of performance of cDNA microarrays, oligomicroarrays
and high throughput sequencing for analysis of gene expression in fish.
-
Established a robust transthyretin binding assay for detection of potential thyroid
hormone disruptors.
-
Standard operating procedures and manual for sampling and quality assessment of the
skeleton in sea bream and sea bass from aquaculture.
108
8d. Productivity
1. Abbink, W, Kulczkowska, E, Kalamarz, H, Guerreiro, PM, Flik, G. 2008.Melatonin synthesis
under calcium constraint in gilthead sea bream (Sparus auratus L.). General and Comparative
Endocrinology 155:94-100. IF=2.654.
2. Barata, EN, Fine, JM, Hubbard, PC, Almeida, OG, Frade, P, Sorensen PW, Canario AVM. 2008.
A sterol-like odorant in the urine of Mozambique tilapia males likely signals social dominance to
females. Journal of Chemical Ecology 34:438-449. IF=2.327.
3. Barata, EN, Serrano, RM, Miranda, A, Nogueira, R, Hubbard, PC, Canario, AVM. 2008. Putative
pheromones from the anal glands of male blennies attract females and enhance male
reproductive success. Animal Behaviour 75:379-389. IF=2.828.
4. Bender, N, Heg-Bachar, Z, Oliveira, RF, Canario, AVM, Taborsky, M. 2008. Hormonal control of
brood care and social status in a cichlid fish with brood care helpers. Physiology & Behavior
94:349-358. IF=2.806.
5. Bender, N, Taborsky, M, Power, D. 2008. The Role of prolactin in the regulation of brood care
in the cooperatively breeding fish neolamorologus pulcher. Journal of Experimental Zoology Part
A - Ecological Genetics and Physiology 309A: 515-524. IF=1.549.
6. Bevelander, GS, Pinto, SLCP, Canario, AVM, Spanings, T, Flik, G. 2008. CYP27A1 expression in
gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related
protein. Journal of Endocrinology 196:625-635. IF=2.791.
7. Canario, AVM, Bargelloni, L, Volckaert, F, Houston, RD, Massault, C, Guiguen, Y. 2008.
Genomics Toolbox for Farmed Fish. Reviews in Fisheries Science 16: 3-15. IF=2.375.
8.Ferraresso, S, Vitulo, N, Mininni, AN, Romualdi, C, Cardazzo, B, Negrisolo, E, Reinhardt, R,
Canario, AVM, Patarnello, T, Bargelloni, L. 2008. Development and validation of a gene
expression oligo microarray for the gilthead sea bream (Sparus aurata). BMC Genomics 9: 580.
IF=3.926.
9. Galay-Burgos, M, Power, DM, Llewellyn, L, Sweeney, GE. 2008. Thyroid hormone receptor
expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus). Molecular
and Cellular Endocrinology 281: 56-63. IF=3.611.
10. Hildahl, J, Power, DM, Björnsson, BT, Einarsdóttir, IE. 2008. Involvement of growth hormoneinsulin-like growth factor I system in cranial remodeling during halibut metamorphosis as
indicated by tissue- and stage-specific receptor gene expression and the presence of growth
hormone receptor protein. Cell and Tissue Research 332: 211-225. IF=2.740.
11. Hirschenhauser, K, Canario, AVM, Ros, AFH, Taborsky, M, Oliveira, RF. 2008. Social context
may affect urinary excretion of 11-ketotestosterone in African cichlids. Behaviour 145: 13671388. IF=1.556.
109
12. Huertas, M, Canario, AMV, Hubbard, PC. 2008. Chemical communication in the Genus
Anguilla: a miniriview. Behaviour 145: 1389-1407. IF=1.556.
13. Kolmakov, NN, Kube, M, Reinhardt, R, Canario, AV. 2008. Analysis of the goldfish Carassius
auratus olfactory epithelium transcriptome reveals the presence of numerous non-olfactory
GPCR and putative receptors for progestin pheromones. BMC Genomics 9: 429. IF=3.926.
14. Morgado, I, Melo, EP, Lundberg, E, Estrela, NL, Sauer-Eriksson, E, Power, DM. 2008.
Hormone affinity and fibril formation of piscine transthyretin: the role of the N-terminal.
Molecular and Cellular Endocrinology 295: 48-58. Evaluated by Faculty of 1000 Biology,
http://www.f1000biology.com/article/id/1118796/evaluation. IF=3.611.
15. Power, DM, Einarsdóttir, IE, Pittman, K, Sweeney, GE, Hildahl, J, Campinho, MA, Silva, N,
Sæle, O, Galay-Burgos, M, Smáradóttir, H, Björnsson, BT. 2008. The molecular and endocrine
basis of flatfish metamorphosis. Reviews in Fisheries Science 16(S1): 95-111. IF=2.375.
16. Saverwyns, H, Visser, A, Van Durme, J, Power, D, Morgado, I, Kennedy, MW, Knox, DP,
Schymkowitz, J, Rousseau, F, Gevaert, K, Vercruysse, J, Claerebout, E, Geldhof, P. 2008. Analysis
of the transthyretin-like (TTL) gene family in Ostertagia ostertagi - Comparison with other
strongylid nematodes and Caenorhabditis elegans. International Journal for Parasitology 38:
1545-1556. IF=3.752.
17. Scott, AP, Hirschenhauser, K, Bender, N, Oliveira, R, Earley, RL, Sebire, M, Ellis, T, Pavlidis, M,
Hubbard, PC, Huertas, M, Canario, A. 2008. Non-invasive measurement of steroids in fishholding water: important considerations when applying the procedure to behaviour studies.
Behaviour 145: 1307-1328. IF=1.556.
18. Serrano, RA, Lopes, O, Hubbard, PC, Araújo, J, Canario, AVM, Barata, EN. 2008. 11Ketotestosterone Stimulates Putative Sex Pheromone Production in the Male Peacock Blenny,
Salaria pavo (Risso 1810). Biology of Reproduction 79: 861-868. IF=3.469.
19. Serrano, RM, Barata, EN, Birkett, MA, Hubbard, PC, Guerreiro, PS, Canario AVM. 2008.
Behavioral and olfactory responses of female Salaria pavo (Pisces : Blenniidae) to a putative
multi-component male pheromone. Journal of Chemical Ecology 34: 647-658. IF=2.327.
20. Serrano, RM, Lopes, O, Hubbard, PC, Araújo, J, Canario, AVM. 2008. Seasonal cell
differentiation and olfactory potency of secretions by the anal glands of male peacock blenny
Salaria pavo. Journal of Fish Biology 73: 1790-1798. IF=1.246.
21. Volckaert, FAMJ Barbier, M, Canario, AVM, Olsen, JL, Wesnigk, J. 2008. Empowering marine
science through genomics. Marine Genomics 1: 33-35.
22. Tanguy, A, Bierne, N, Saavedra, C, Pina, B, Bachère, E, Kube, M, Bazin, E, Bonhomme, F,
Boudry, P, Boulo, V, Boutet, I, Cancela, L, Dossat, C, Favrel, P, Huvet, A, Jarque, S, Jollivet, D,
Klages, S, Lapègue, S, Leite, R, Moal, J, Moraga, D, Reinhardt, R, Samain, JF, Zouros, E, Canario, A.
2008. Increasing genomic information in bivalves through new EST collections in four species:
development of new genetic markers for environmental studies and genome evolution. Gene
408: 27-36. IF=2.578.
110
proceedings)
Books (Chapters)
1. Power, DM, Silva, N, Campinho, MA. 2008. Metamorphosis. In: Flynn, RN, Kapoor, BG (eds).
Fish Larval Physiology. Science Publishers, Enfield, UK. Chapter 19, pp. 1-33.
vertebrates. In: Oliveira, RF, Taborsky, M, Brockmann, HJ (Eds). Alternative Reproductive Tactics:
an Integrative Approach. Cambridge University Press, pp. 132-173. ISBN 978-0-521-83243-4.
Edited volumes
3. Sundell, K, Power, DM (Eds). 2008. The use of molecular methods and functional genomics in
aquaculture. Special issue Reviews in Fisheries Science Volume 16, Issue S1.
4. Canário, AVM, Scott, AP, Hubbard, PC, Barata, EN (Eds). 2008. Bioactive water-borne
chemicals: pheromones and welfare indicators - the Faro Workshop. Special Issue Behaviour
Volume 145, Number 10.
Conference proceedings
5. Haond, C, Canario, AVM. 2008. Re-Examination of the Immunolocalization of the Cystic
Fibrosis Transmembrane Regulator in Mouse Kidney: Evidence for Its Basolateral Location in
Renal Epithelial Cells. Takis Anagnostopoulos Symposium: Renal and Epithelial Physiology and
Pathophysiology, 26-27 June 2008 , Hôpital Necker, Paris, France. Nephron Physiol 2008;110,
p.29.
6. Huertas, M, Canario, AVM, Hubbard, PC. 2008. Changes in Olfactory Sensitivity During the
European Eel (Anguilla Anguilla) Life Cycle. XV International symposium on olfaction and taste.
San Francisco, USA. Chem. Senses 33, S150-S150.
proceedings)
MASTER THESES
Jordão, V. 2008. Tolerância à amonia e grau de ureoltelia no xarroco, Halobatrachus dudactylus
(Bloch & Schneider, 1801). Master thesis in Marine Biology, University of Algarve.
Bicho, R CSS. 2008. Efeito do cloro no desenvolvimento e no eixo tiroide da Tilapia
Mocambicana (Oreochromis mossambicus). Master thesis in Biotechnology, University of
Algarve.
111
Guerreiro, E. 2008. Thyroid Axis disruption by goitrogens: a molecular and functional approach.
Master thesis in Biological Engineering, University of Algarve.
Gregoria, S. 2008. Molecular and cellular analysis of the regenerative response of skin in a fish
model. Master thesis in Biological Engineering, University of Algarve.
Rosa, I. 2008. Influence of high stocking density, fasting and refeeding on intermediate
Algarve.
Batista, S. 2008. The effect of the replacement of fishmeal to algae flour on growth and
Carvalho, E. 2008. The influence of disruption of the thyroid hormone axis on skin: a morphofunctional study. Master thesis in Marine Biology, University of Algarve.
PhD THESES
Delgado, C. 2008. Gonad development and hormone titres in loggerhead sea turtles Caretta
caretta in the NE Atlantic. PhD thesis, University of Madeira.
Serrano, RM. 2008. Pheromones in the reproduction of Salaria pavo and S. fluviatilis (Pisces:
Blenniidae): a comparative study. PhD thesis, University of Évora.
Workshop in Microarray. In the context of the EU project (FP6-2005-SSP-5-A), Genomics in fish
and shellfish: From research to aquaculture (AQUAGENOME). March 2008, Gothenburg,
Sweden.
In 2008 the group published with colleagues from 13 countries and 25 universities and research
institutes from Europe and the USA. LIFECYCLE is an FP7 Large Scale Integrating Project with 14
partners (http://www.lifecycle-fp7.eu/). The international standing of the group leader can be
inferred from participation in ASSEMBLE (http://assemblemarine.org/ ) a FP7 Research
Infrastructure (I3) and the recently approved ESFRI infrastructure European Marine Biological
112
Resource Centre (http://www.embrc.eu/). Group members participate in COST Action networks
925 and 867 dedicated, respectively, to “The importance of prenatal events for postnatal muscle
growth in relation to the quality of muscle based foods” (D Power) and “Welfare of Fish in
European Aquaculture” (P Guerreiro). Participation and organization of events in Aquagenome
FP6 specific support action (D Power). Invited lectures to Aquaculture Europe 2008, 15-18
September , Krakow, Poland (M Huertas) and 24th Conference of European Comparative
Endocrinologists (CECE), 2-6 September, Genoa, Italy (A Canario). Researchers from Spain (Univ
Murcia, Univ Barcelona, Univ Cadiz), Sweeden (Univ Goteborg), UK (British Antarctic Survey) and
Brazil (Univ Fed Rio Grande do Sul) made research visits to our group (1 week – 5 months).
Researchers from our group made research visits to Denmark (Univ Copenhagen), Sweeden
(Univ Goteborg), Brazil (Univ Fed Rio Grande do Sul), Spain (Univ Murcia, Univ Cadiz), Italy (Univ
Padova) for periods of 1 week to 3 months. D Power is associated editor of General Comparative
Endocrinology; A Canario is Advisory editor of Acta Ethologica. Senior members of the group
reviewed more than 30 manuscripts for international journals. Reviewed project applications for
the European Comission and programmes in several countries (Argentina, France, Spain, UK, The
Netherlands, USA).
8e. Future research
1. Objectives
The objectives below are medium term (5 years), for which we have either secured funding or
have applied for funding:
-
What genes determine sex in fish? The focus is on the identification of early genes of the
cascade of sex differentiation using transcriptomic and transgene approaches, with a
view of understanding how environmental factors such as temperature can modify sex
ratios.
-
What signals direct fish to grow or to mature? There are metabolic, behavioural and
neuroendocrine components which need to be dissected using global transcriptomics
approaches to help us understand the mechanisms underlying the development of
alternative mating tactics in fish and other vertebrates as well as precocious puberty in
farmed fish.
-
Develop a model system for characterisation of TH on organogenesis and functional
modifications during fish metamorphosis in order to study how asymmetry is generated.
Establish the role of TH receptors, deiodinases and TH binding proteins and cell
transporter proteins in regulating TH tissue responsiveness during fish metamorphosis.
-
To establish a model for the mechanism of action and physiological roles of the
parathyroid (PTH) family of peptides in fish. Identification of signaling pathways,
downstream responsive genes in key ion regulatory tissue (bone, intestine and kidney)
113
and upstream biological responses. Physiological techniques, transcriptome and
proteome analysis, RNA interference and transgenic approaches will be used.
-
Deorphanisation and functional characterisation of potential calcitonin/calcitonin
related peptide and PTH-like proteins and their receptors in invertebrates and
vertebrates. The approach includes physiological studies, RNA interference, gene
expression and ligand peptide screening.
-
Establish how pheromones regulate reproductive and social interactions in fish by
determining the chemical nature of pheromones, site of production and regulation of
synthesis and their mode of action. Electrophysiology, behaviour, chemistry and
genomics approaches will be used to study this question. Identification of ligands for
olfactory receptors, the signaling pathways and mapping of olfactory neurones to
olfactory bulb centers are some of the expected outcomes.
-
Identification of odorants present in natural diets of economically important fish, which
may be used as attractants in additives to inert-feed for improvement of feed ingestion,
growth and feed conversion rates in aquaculture. This work is particularly directed at
Solea senegalensis, a new species for aquaculture for which feeding and the production
of attractive dry diets is a bottleneck.
2.

Current funding
-
Source: European Commission
160.400 EUR, FP6 nº 012451, 2005-2009.
366.304 EUR, FP7 project nº 222719, 2009-2012.
-
Source: FCT
25.000 EUR/year, FCT Pluriannual funding.
185.091 EUR, PTDC/CVT/66735/2006, 2007-2010.
10.944 EUR, PTDC/PSI/71811/2006, 2007-2010.
7.200 EUR, PTDC/MAR/72117/2006, 2008-2011.
69.592 EUR, PTDC/MAR/69749/2006, 2008-2011.
18.000 EUR, PTDC/MAR/71351/2006, 2008-2011.
-
Source: Others
30.000 EUR; several sources and services.

114
146.388 EUR, FCT PTDC/MAR/103781/2008.
199.416 EUR, FCT PTDC/CVT/098040/2008.
151.056 EUR, FCT PTDC/MAR/108063/2008.
151.672 EUR, FCT PTDC/BIA-BCM/103446/2008.
150.874 EUR, FCT PTDC/CVT/103378/2008.
166.464 EUR, FCT PTDC/CVT/098200/2008.
178.224 EUR, FCT PTDC/MAR/104008/2008.
50.000 EUR, SOBREIRO/0025/2009.
125.691 EUR, FCT PTDC/BIA-BEC/099712/2008.
8.400 EUR, FCT PTDC/MAR/099534/2008.
162.283 EUR, FCT PTDC/CVT/102178/2008.
123.468 EUR, FCT PTDC/MAR/108231/2008.
156.308 EUR, FCT PTDC/CVT/101600/2008.
147.852 EUR, FCT PTDC/MAR/099893/2008.
115
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)
Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)
Luis Manuel Zambujal Chicharo
Pluriannual FCT funding.
600.000 EUR, INTERREG, EURSOURCE - Development and harmonization of new indicators,
methodologies and strategies common for Portugal and Spain for the application of the Water
Frame Directive to transitional and coastal water mass in the Guadiana- DIMEAGUA. Feb 2009 Jan 2011.
199.962,90 EUR, ERA-NET CIRCLE - MED project. CIRCLE MED - FCT Portugal. Italian Minister for
Environment, Land and Sea (IMELS), Italy; Regional Ministry of Innovation and Industry of the
Galician Government trough the Directorate General for Research, Development and Innovation
(XUNTA), Spain. The integrated impacts of marine acidification, temperature and precipitation
changes on coastal biodiversity and fisheries: how to adapt? (ACID-BIV). Oct 2008 - Sept 2010.
8.000,00 EUR, FCT - Bilateral collaborative project FCT/MESRST project (Portugal-Tunisia):
Coastal ecology and global changes. A comparative study between Guadiana and Medjerda
coastal zones (GUAMED). Funding for 8 short scientific visits (1 week each) exchange between
researchers from both institutions (CCMAR and Sciences Faculty of Bizerta – FSB). Oct 2008 Sept 2010.
1. Objectives
Our research is mainly focused on biological and physical-chemical processes in transitional
waters, from freshwater catchments to coastal marine ecosystems. We are particularly
interested in primary and secondary production, nutrient cycles, the characterisation of
habitats, populations, trophic interactions and community dynamics. The purpose of our
research is to understand the natural variability of aquatic ecosystems (e. g., tides, upwelling,
116
winds) and the ecologic impacts by caused by human activities (fisheries, pollution, dams, alien
species, etc). All this information is necessary to develop and test Ecohydrology ecosystembased solutions for reverting degradation, sustaining functioning and promoting adaptation of
aquatic environments to impacts, considering the current scenarios for climatic changes.
In the field of estuarine ecology an understanding the threats of invasive species to estuarine
ecosystems, especially in the Guadiana estuary, was achieved. The routine determination of
Secondary production in this estuary by copepods egg methods was also accomplished.
In the field of ecology of fish larvae the major achievement of the group was the analysis of the
RNA:DNA ratios as condition indicator and its relation with swimming abilities of larvae and its
implications to recruitment.
In the field of global changes it was set up an experimental approach to analyse the effects of
marine acidification on invertebrates, such as bivalves.
8d. Productivity
1. Garrido, S, Ben-Hamadou, R, Oliveira, PB, Cunha, ME, Chícharo, MA, van der Lingen, CD. 2008.
Diet and feeding intensity of sardine Sardina pilchardus: correlation with satellite-derived
chlorophyll data. Marine Ecology Progress Series 354: 245-256. IF=2.631.
2. Garrido, S, Rosa, R, Ben-Hamadou, R, Cunha, ME, Chícharo, MA, van der Lingen, CD. 2008.
Spatio-temporal variability in fatty acid trophic biomarkers in stomach contents and muscle of
Iberian sardine (Sardina pilchardus) and its relationship with spawning. Marine Biology 154:
1053-1065. IF=1.953.
3. Moschino, V, Marin, MG, Chícharo, L. 2008. Effects of hydraulic dredging on the physiological
responses of the target species Chamelea gallina (Mollusca, Bivalvia): laboratory experiments
and field surveys. Scientia Marina 72(3): 493-501. IF=1.075.
4. Chicharo, MA, Chicharo, L. 2008. RNA:DNA ratio and other nucleic acid derived indices in
Marine Ecology. International Journal of Molecular Sciences 9: 1453-1471. IF=0.978.
their conservation. Estuaries and Coasts 31: 1198-1207. IF=1.408.
6. Morais, P. 2008. Review on the major ecosystem impacts caused by damming and watershed
development in an Iberian basin (SW-Europe) - focus on the Guadiana estuary. Annales de
Limnologie - International Journal of Limnology 44(2): 69-81. IF=0.768.
117
7. Batista, FM, Ben-Hamadou, R, Fonseca, VG, Taris, N, Ruano, F, Henriques, MA, Boudry, P.
2008. Comparative study of shell shape and muscle scar pigmentation in the closely related
cupped oyster taxa Crassostrea angulata, C. gigas and their reciprocal hybrids. Aquatic Living
Resources 21: 31-38. IF=0.667.
8. Sandra, J, Gaspar, MB, Matias, D, Ben-Hamadou, R, Arnold, WS. 2008. Rebuilding viable
spawner patches of the overfished Spisula solida (Mollusca: Bivalvia): a preliminary contribution
to fishery sustainability. ICES Journal of Marine Science 65: 60-64. IF=1.661.
9. Range, P, Chapman, MG, Underwood, AJ. 2008. Field experiments with "cageless" methods to
manipulate grazing gastropods on intertidal rocky shores. Journal of Experimental Marine
Biology and Ecology 365: 23-30. IF=2.074.
proceedings)
Books (Chapters)
1. Wolanski, E, Chicharo, L, Chicharo, MA. 2008. Estuarine Ecohydrology. In: Jørgensen, SE, Fath,
BD (Editor-in-Chief). Ecological Engineering. Vol. 2 of Encyclopedia of Ecology, 5 vols. pp. 14131422. Oxford. Elsevier.
Conference Proceedings and abstracts:
2. Ben-Hamadou, R, Chicharo, A, Madeira, C, Morais, P, Cruz, J, Chicharo, L. 2008. Zooplankton
distribution in Algarve coastal zone (North East Atlantic Ocean). Abstract - WKZEM CIESM-ICES
join
workshop.
Heraklion,
Greece,
October
2008.
(http://www.st.nmfs.noaa.gov/plankton/wkzem/frame_abstracts/index.html)
3. Berecibar, E, Ben Hamadou, R, Tavares, M, Santos, R. 2008. Long term changes in the
phytogeography of the Portuguese continental coast. Proceedings of the 43rd Estuarine &
Coastal Sciences Association International Symposium, Lisbon, Portugal, 7-9 February 2008.
(http://www.siam.fc.ul.pt/ECSA2008/7FEBPDF/BerecibarE.pdf)
phytogeography of the Portuguese continental coast. Abstract: 2008 ASLO Summer Meeting, 81
June,
St
John’s,
Newfoundland
&
Labrador,
Canada.
(http://www.sgmeet.com/aslo/stjohns2008/viewabstract2.asp?AbstractID=3809)
proceedings)
118
MASTER THESES
Leitão, TA. 2008. Efeito do caudal na estrutura das comunidades de macrocrustaceos no
estuário do Guadiana. Master thesis, University of Algarve. Supervisors: MA Chicharo, L
Chicharo.
PhD THESES
Morais, PM. 2008. Engraulis encrasicolus sensus lato population dynamics in the Guadiana
estuary and adjacent coastal area. PhD thesis, University of Algarve. Supervisors: MA Chicharo, L
Chicharo.
III Encontro Nacional de Estudantes de Pós-Graduação em Ciências Biológicas. Faro, 3-5
September 2008.
Collaborative publications:
-
Moschino, V, Marin, MG, Chícharo, L. 2008. Effects of hydraulic dredging on the
physiological responses of the target species Chamelea gallina (Mollusca, Bivalvia):
laboratory experiments and field surveys. Scientia Marina 72(3): 493-501.
Publication based on collaborative research between Univ. Padova (Italy) and
CCMAR.
-
Olivar, P, Chicharo, M, Diaz, M. (in press). Tissue effect on RNA:DNA ratios of marine
fish larvae. Scientia Marina. Based on collaborative research between Institut de
Ciències del Mar (Spain), Instituto Nacional de Investigación y Desarrollo Pesquero
(Argentina) and CCMAR.
Research Project ACID BIV - CIRCLE-MED, coordinated by CCMAR (L Chícharo); in collaboration
with CSIC (Vigo, Spain), Department of Biology – Univ. Padova, Faculty of Science of Bizerta
(Tunisia).
Bilateral collaborative project approved in 2008:
119
-
Coastal ecology and global changes. A comparative study between Guadiana and
Medjerda coastal zones (GUAMED). A bilateral FCT/MESRST project (PortugalTunisia). Funds for exchange of researcher and graduated students approved and
provided by: GRICES (Portugal), MESRST (Tunisia). Partners: ECOREACH, CCMAR and
Faculty of Science of Bizerta (Tunisia).
-
INTERRE project “DIMEA UA”, coordinated by CCMAR (Luis Chícharo); in
collaboration with IFAPA, Centro “Agua del Pino” (Spain).
ECOREACH members participate in several national and international scientific networks,
mainly:
-
DivMar: a National Biodiversity Network for Portuguese marine and coastal biodiversity
established in 2007.
-
MedZoo: MedZoo is an association of scientists willing to collaborate on the
harmonisation of methods for study of the evolution of Mediterranean and Black Sea
zooplankton in time and space. Medzoo was created during the 37th CIESM congress in
Barcelona in June 2004 and is functioning through the CIESM program on Zooplankton
Indicators.
-
WssTP: The Water supply and sanitation Technology Platform (WssTP) is a European
initiative for European Research and Technology Development in the water industry.
Members of ECOREACH participated in the CLIMECO workshop - Climate driving of marine
ecosystem changes, training for young marine scientists. 21-24 April, IUEM, Brest (France) and
also collaborated in developing a new method for zooplankton research (ZOOSCAN) with the
“Laboratoire d´Océanographie de Villefranche-sur-mer” - Université Paris VI.
8e. Future research
1. Objectives
Global change in coastal ecosystems - Impacts of alien species on coastal ecosystems, and
Effects marine acidification on fisheries and aquaculture.
Fish larvae trophodynamics - New trophic links between fish larvae and plankton will be also a
topic for research, especially alternative links between bacteria-phytoplankton and ciliates,
instead of traditional phyto-nauplii-fish larvae links.
The projected research is intended to be formally associated with the activity of a future
International Centre for Coastal Ecohydrology (ICCE) under the UNESCO auspices, at the
University of Algarve and having as an objective a close collaboration in fomenting and better
120
disseminate research in coastal sciences with the CIMAR LA. This collaboration would enhance
the internationalisation of the produced research and applications and build synergetic
partnership with regional and international water related stakeholders.
2.

Current funding
Pluriannual FCT funding.
600.000 EUR, INTERREG, EURSOURCE - Development and harmonization of new indicators,
methodologies and strategies common for Portugal and Spain for the application of the WFD to
transitional and coastal water mass in the Guadiana - DIMEAGUA. Feb 2009 - Jan 2011.
199.962,90 EUR, ERA-NET CIRCLE - MED project. CIRCLE MED - FCT Portugal. The integrated
impacts of marine acidification, temperature and precipitation changes on coastal biodiversity
and fisheries: how to adapt? (ACID-BIV). Oct 2008 - Sept 2010.
8.000,00 EUR, FCT - Bilateral collaborative project FCT/MESRST project (Portugal-Tunisia):
Coastal ecology and global changes. A comparative study between Guadiana and Medjerda
coastal zones (GUAMED). Oct 2008 - Sept 2010.

FCT PTDC/AAC-AMB/102894/2008, Impact of aquatic invertebrate alien species in estuaries
ecosystems - Guadiana as a study case.
FCT PTDC/MAR/104798/2008, Trophic interactions in marine benthic environment: assessment
of dredging effects and of recovery trajectories.
FCT PTDC/BIA-BEC/099252/2008, Improving knowledge on processes in early phases of
estuarine fishes.
FCT PTDC/MAR/108325/2008, Environmental drivers of phytoplankton in marine critical
transition systems - PHYTODRIVE.
FCT PTDC/BIA-BEC/101838/2008, Parental effects in sardines with emphasis to the role of
essential fatty acids for larvae survival - PARFISH.
FCT PTDC/MAR/108238/2008, An integrated approach to the environmental study of climate
change impacts on plankton communities - ZOOCLIM.
FCT PTDC/MAR/108777/2008, Cytogenetic endpoints for environmental genotoxicity
assessment of Guadiana and Ria Formosa Coastal Ecosystems (South Portugal) - ECOCYTO.
121
Ecophysiology
Ecophysiology
CIIMAR -Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto
Funding spent in 2008:
-
-
National funding
2.334,34 EUR, FCT PTDC/AMB/70431/2006.
4.231,46 EUR, FCT POCI/MAR/58837/2004.
1.198,09 EUR, FCT POCI/MAR/55547/2004.
10.190,30 EUR, FCT POCI/MAR/57022/2004.
1.874,78 EUR, FCT POCI/MAR/60365/2004.
38.048,89 EUR, FCT PTDC/MAR/64016/2006.
7.071,27 EUR, FCT POCI/CLI/61605/2004.
-
European funding
12.454,95 EUR, 6 FP-EU, AMPERA.
22.818,64 EUR, EEA Grants, PT010.
4.127,08 EUR, INTERREG IIIA, PROTEUS.
-
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Private funding
25.684,31 EUR, Amb & Veritas.
-
Other funding
3.435,02 EUR, University of Porto - Pre-graduation scientific research.
5.552,00 EUR, Ciência Viva - Biologia no Verão.
1. Objectives
The objectives of the group have not changed since the 2003-2007 report was put together. For
2008 they are restated as follows:
-
Investigate the physiological challenges faced by migratory fishes (Anguilla anguilla,
Platichthys flesus, Petromyzon marinus, Scyliorhinus cannicula, Pagellus bogaraveo),
focusing on the abiotic factors of salinity and hydrostatic pressure. The species that are
investigated are of commercial and/or biological significance.
-
Determine migratory patterns of fishes (A. anguilla, Conger conger, P. flesus) at different
life history stages using otolith fingerprinting and complementary techniques.
Establishing techniques and continuing monitoring of European eel (A. anguilla)
recruitment levels.
-
Defining the mechanisms for dealing with physiological constraints and challenges of
ammonotelism in fishes. Ammonia can be highly toxic to animals yet is a natural
byproduct of metabolism.
-
Elucidate the mechanism of shell calcification in freshwater bivalves (Anodonta cygnea).
In addition, the definition of culture conditions for freshwater bivalves (A. cygnea,
Hyriopsis myersiana).
-
Technological advances to aid in biological discovers and monitoring of the
environment. Specifically the development of methods for the non-invasive monitoring
of physiological parameters and the development of hyperbaric systems for the
maintenance of fish and other aquatic organisms for investigating hydrostatic pressure
effects on physiology.
-
Identification of ammocoete metamorphosis as the critical time in the acquisition of
marine ionoregulatory competence in the anadromous marine lamprey (Petromyzon
marinus).
-
Identify and characterize the principal biomineralization events on freshwater bivalve
Anodonta cygnea. This includes the identification and characterization of mantle cell
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proteins involved in intercellular calcium binding and transport, extrapalial fluid
macromolecules involved in the shell calcification mechanisms and the identification of
the physical site (tissue) associated with the active calcium uptake from the
environment.
-
Improvement of freshwater bivalve larval glochidia culture and juvenile production.
Freshwater pearl mussel Hyriopsis myersiana and Anodonta cygnea for culture and
conservation purposes.
-
inclusion in EU level networks and participation in other national and international
collaborative projects.
-
Dissemination of results generated by the group. In 2008 we collectively have 11
international peer reviewed publications in addition to three other publications. We also
have 13 conference presentations, 5 of which were paper presentations. Group
members have also been actively involved in education of the public.
-
Research training at the undergraduate and graduate levels (2MSc, 1 PhD completed in
2008).
8d. Productivity
1. Faubel, D, Lopes-Lima, M, Freitas, S, Pereira, L, Andrade, J, Checa, A, Frank, H, Matsuda, T,
Machado, J. 2008. Effects of Cd2+ on the calcium metabolism and shell mineralization of bivalve
Anodonta cygnea. Marine and Freshwater Behaviour and Physiology 41: 93-108. IF=1.271.
2. Kadar, E, Checa, AG, Damasceno-Oliveira, A, Machado, JP. 2008. Shell nacre ultrastructure
and depressurisation dissolution in the deep-sea hydrothermal vent mussel Bathymodiolus
azoricus. Journal of Comparative Physiology B 178(1): 123-130. IF=1.698.
3. Kovitvadhi, S, Kovitvadhi, U, Sawangwong, P, Machado, J. 2008. A laboratory-scale
recirculating aquaculture system for juveniles of freshwater pearl mussel Hyriopsis
(Limnoscapha) myersiana (Lea, 1856). Aquaculture 275: 169-177. IF=1.678.
4. Lopes-Lima, M, Bleher, R, Forg, T, Hafner, M, Machado, J. 2008. Studies on a PMCA-like
protein in the outer mantle epithelium of Anodonta cygnea: insights on calcium transcellular
dynamics. Journal of Comparative Physiology B-Biochemical Systemic and Environmental
Physiology 178: 17-25. IF=1.698.
5. Reis-Santos, PN, McCormick, SD, Wilson, JM. 2008 Ionoregulatory changes during
metamorphosis and salinity exposure of juvenile sea lamprey (Petromyzon marinus L.). Journal
of Experimental Biology 211: 978-988. IF=2.981.
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fluminea (Muller, 1774) in aquatic ecosystems: an overview. Annales de LimnologieInternational Journal of Limnology 44: 85-94. IF=0.768.
the River Minho estuarine gradient (north-west Iberian Peninsula). Aquatic Conservation-Marine
and Freshwater Ecosystems 18: 1063-1077. IF=1.500.
(Muller, 1774; Bivalvia: Sphaeriidae) distribution in the River Minho Estuary: consequences for
its conservation. Estuaries and Coasts 31: 1198-1207. IF=1.408.
9. Sousa, R, Nogueira, AJA, Antunes, C, Guilhermino, L. 2008. Growth and production of Pisidium
amnicum in the freshwater tidal area of the River Minho estuary. Estuarine Coastal and Shelf
Science 79: 467-474. IF=2.072.
10. Sousa, R, Nogueira, AJA, Gaspar, MB, Antunes, C, Guilhermino, L. 2008. Growth and
extremely high production of the non-indigenous invasive species Corbicula fluminea (Muller,
1774): Possible implications for ecosystem functioning. Estuarine Coastal and Shelf Science 80:
289-295. IF=2.072.
Portugal. Aquatic Conservation-Marine and Freshwater Ecosystems 18: 98-110. IF=1.500.
threats to faunal biodiversity Aquatic Biology 3: 237-250. IF=0.762.
13. Morais, P. 2008. Review on the major ecosystem impacts caused by damming and
watershed development in an Iberian basin (SW-Europe) - focus on the Guadiana estuary.
Annales de Limnologie - International Journal of Limnology 44(2): 69-81. IF=0.768.
14. Cunha, SR, GonçalveS, R., Silva, SR, Correia, AD. 2008. An automated marine biomonitoring
system for assessing water quality in real-time. Ecotoxicology 17: 558-564. IF=2.355.
proceedings)
1. Gonçalves, JFM, Damasceno-Oliveira, AP. 2008. Aquaculture project formulation. In: Pham,
CK, Higgins, RM, De Girolamo, M, Isidro, E (Eds). Proceedings of the International Workshop:
Developing a Sustainable Aquaculture Industry in the Azores, pp. 25-29. Arquipélago. Life and
Marine Sciences. Supplement 7: xiii + 81 pp. ISBN: 978-972-8612-44-3.
2. Prouzet, P, Bouvet, J-C, Bru, N, Duquesne, E, Antunes, C, Damasceno-Oliveira, A, Boussouar, A,
De-Casamajor, M-N, Sanchez, F, Lissardy, M. 2008. Indicateurs de recrutement estuarien. In:
Gilles Adam, Eric Feunteun, Patrick Prouzet and Christian Rigaud (Eds). L'anguille européenne Indicateurs d'abondance et de colonization. Éditions Quae, Versailles, France. ISBN: 978-2-75920085-6.
125
3. Letão, A, Damasceno-Oliveira, A, Pereira, CM, Coimbra, JC, Wilson, JM. 2008. Transport stress
in glass eels. In: JA Muñoz-Cueto, JM Mancera, G Martínez-Rodríguez (Eds). Avanços em
Endocrinologia Comparativa, Vol 4, University of Cadiz, pp 63-68. ISBN978-84-9828-152-1.
proceedings)
MASTER THESES
Barros, F. 2008. Utilização de assinaturas químicas elementares e isotópicas no estudo do ciclo
de vida do congro Europeu, Conger conger. Master thesis in Applied Ecology, Faculty of
Sciences, University of Porto. Supervisor: AT Correira.
Gonçalves, O. 2008. Gastric proton pump expression in teleost fishes: nongastric sites of
Douro (UTAD). Supervisor: J Wilson, Co-supervisor: Antonio Fontaínhas Fernandes (UTAD).
PhD THESES
Rocha, AM. 2008. Clonação, caracterização funcional e expressão dos receptores das
gonadotrofinas (FSH e LH) no robalo europeu (Dicentrarchus labrax, L.). PhD thesis, CSIC,
University of Sevilha. Co supervior: J Coimbra.
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Guilhermino, Cosupervisor: C Antunes.
Portable equipment for measuring and calculating locomotion dynamic parameters, National
patent request no. 103933, Jan 2008. M Correia, S Cunha.
International Congress Seasink 2008. University Fernando Pessoa, 26-28 July 2008, Porto,
Portugal.
-
Organization of conferences in 2009:
7th AIEC Congress, Iberian Association of Comparative Endocrinology, 6-9 September 2009,
Porto, Portugal.
BIOLIEF, World conference on Biological Invasions and Ecosystem Functioning, 27-30 October
2009, Porto, Portugal.
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Contract research with:
-
Amb. & Veritas, for the project “Prospecção de Dep sitos de Inertes na Costa Ocidental
e Algarvia”. Total amount of 137.700 EUR (25.684,31 EUR in 2008)
-
EDP, iodiversity fund, for the project “Plano nacional de conservação da lampreia-derio e da lampreia-de-riacho”. Total amount of 115.000 EUR, contract signed in Nov.
2008.
In 2008, the Ecophysiology group had six publications and ten conference presentations with
international collaborators. In addition through collaborations on British Biological Sciences
Research Council (BBSRC, UK) grants with Dr. RW Wilson (University of Exeter), we have been
involved in the advanced training of post-doctoral researchers in Porto. Dr. Jonathan Wilson in
the group is also involved in collaborative research with Prof. Colin Brauner of the University of
British Columbia funded by the Natural Sciences and Research Council (Canada). We also
currently have a post-doctoral researcher (Dr. A Damasceno-Oliveira) with a cross appointment
to the Liverpool Microarray Facility and Centre for BioArray Innovation, University of Liverpool
with Dr. A Cossins. Through provisions in their FCT fellowships, PhD students have worked
abroad (R Sousa: Cambridge UK; JM Moreira Silva; U. Copenhagen, Denmark; AF Gonçalves: U.
Waterloo).
8e. Future research
1. Objectives
Our Future Research Objectives have not changed from the 2003-2007 report.
-
The group will continue to address the questions that arise from the physiological
adaptation of fishes in future work, however, with a clear shift to using transcriptomics.
This approach will be used to study changes in the transciptome of maturing (silver)
European eel and rainbow trout during hydrostatic pressure adaptation. The European
eel is reasonably pressure tolerant while the rainbow trout is shallow water species that
is relatively pressure intolerant. The physiological study of the ontogeny of ion
regulation will continue in lamprey as well as mechanisms of ammonia tolerance in
teleost fishes.
-
Develop further otolith finger printing (use of elemental and isotopic techniques) as
biological tracers of fish migration and for stock discrimination in the lab. Otolith
127
strontium: Calcium ratio analysis is being used to infer the migratory pattern of the
European flounder (Platichthys flesus) in the Minho River estuary. ICPMS (SB and LA)
stock discrimination techniques are also being developed for two sparidae species
(Diplodus vulgaris and Spondyliosoma cantharus).
-
Further elucidate the calcification processes in freshwater bivalves. This includes the
identification and characterization of mantle cell proteins involved in intercellular
calcium binding and transport, extrapalial fluid macromolecules involved in the shell
calcification mechanisms and the identification of the physical site (tissue) associated
with the active calcium uptake from the environment.
-
Further develop in vitro culture conditions for freshwater bivalves for species
conservation and aquaculture.
-
Related to future advances will be the technological aspect of the group. This will
involve improvements and technological developments to the hydrostatic chamber
system (high pressure levels) and for remote monitoring systems.
2.

Current funding
92.848 EUR, FCT PTDC/MAR/64016/2006, 07/2008-06/2011.
29.466 EUR, FCT PTDC/AMB/70431/2006, 1/2008-1/2011.
1.040.875 EUR, EEA Grants, PT010, 2007-2009.
279.359 EUR, INTERREG IV POCTEP, NATURA-MINHO-MIÑO, 2009-2010.
115.000 EUR, EDP - Biodiversity fund, 2009-2011.

Pending funding
154.367 EUR, FCT PTDC/MAR/098035/2008.
107.628 EUR, FCT PTDC/MAR/098038/2008.
199.360 EUR, FCT PTDC/MAR/098168/2008.
191.582 EUR, FCT PTDC/MAR/098190/2008.
107.947 EUR, FCT PTDC/MAR/098380/2008.
196.801 EUR, FCT PTDC/MAR/098396/2008.
131.868 EUR, FCT PTDC/MAR/098780/2008.
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118.554 EUR, FCT PTDC/MAR/101601/2008.
154.925 EUR, FCT PTDC/CVT/099247/2008.
89.304 EUR, FCT PTDC/BIA-BEC/105266/2008.
141.804 EUR, FCT PTDC/AAC-AMB/098824/2008.
112.421 EUR, FCT PRDC/AAC-AMB/099245/2008.
129
Lúcia Maria das Candeias Guilhermino
In 2009, ECOTOX contributed to bring several projects to CIMAR and a total of 356.573,30 EUR
directly to the group: FCT and other research projects (295.858,00 EUR), pluriannual FCT funds
(13.400,00 EUR), students fees (14.300,00 EUR) and other (42.860,00 EUR). In addition, several
members participated in international meetings with traveling/stay expenses directly covered
and several projects are under evaluation (not included in the budget).
Detailed:
-
13.400,00 EUR FCT pluriannual funding.
-
FCT National funding
3.692 EUR (2009), FCT PPCDT/MAR/58244/2004, 2008-2009.
63.271 EUR (2009), FCT PTDC/MAR/71143/2006, 2008-2011. Total funding ECOTOX: 189.814
EUR.
30.128 EUR (2009), EU ERA-AMPERA 0001/2007, 2008-2011. Total funding ECOTOX: 90.384,00
EUR.
198.767 EUR FCT PTDC/AAC-AMB/102121/2008 (new project), 2010-2014.
50.040 EUR, FCT LTER/BIA-BEC/0019/2009 (new project), 2010-2014.
60.000 EUR, FCT PTDC/MAR/108369/2008 (new project), 2010-2014.
-
Others
33.015 EUR (2009) - University, industry, other sources.
130
3.000 EUR, other projects.
14.300 EUR, student fees.
1. Objectives
The central objective of ECOTOX is to investigate the evolution and functioning of aquatic
ecosystems under anthropogenic and natural stress, with emphasis on estuarine and coastal
ecosystems. We are particularly interested in the assessment of long-term alterations induced
by global stressors (e.g. pollution, biological invasions and climate changes) on both biotic and
abiotic components of ecosystems, in understanding how and why these alterations may affect
their structure and functioning and how to achieve a compromise between biodiversity
conservation and sustainable development in global change scenarios. For this, we have been
working in several Portuguese ecosystems and in tropical selected areas, using 3
complementary approaches: comparison of similar ecosystems under different types and/or
levels of pollution (and other global stressors), study over time of the same ecosystem and
comparison of similar problems in temperate and tropical regions. In all cases, integrated
strategies including methods from ecotoxicology, ecology, genetics, and chemistry have been
used.
Specifically we intend to:
-
Characterize relevant ecosystems of the Portuguese coast and selected case studies in
tropical areas, including the identification of key-species and main problems (e.g.
chemical pollution, eutrophication, landscape alterations, climate changes, biological
invasions), and to study their evolution over time.
-
Assess the impact of stressors on organisms, populations and on the whole ecosystem
by using conventional approaches such as ecological and ecotoxicological monitoring,
bioaccumulation and biomagnification studies, ecological risk assessment and new
methods developed by the group (e.g. in situ and laboratory bioassays with native
species, environmental biomarkers).
-
Understand the mechanisms of action of stressors at sub-individual, individual and
population levels and how the presence of stressors can influence species relationships
(e.g. predation, competition), biotic/abiotic interactions, energy transfer and nutrient
cycling in ecosystems.
-
Contribute for the achievement of a compromise between biodiversity conservation and
sustainable development by providing tools, results and recommendations to decisionmakers supporting scientific-based environmental policies and ecosystem management.
-
12 full papers published, 6 in press in ISI indexed journal.
131
-
2 book chapters, several other national publications.
-
More than 30 presentations in scientific meetings, the most part of them with published
abstracts.
-
1 new ecotoxicity lab bioassays linking behaviour with biomarkers (marine organisms).
-
Validation of 2 native species (invertebrates, reptiles) biomarkers for use in
biomonitoring studies in temperate and tropical ecosystems.
-
Cholinesterases characterization in 2 species and their responses to reference
pollutants.
-
Effects of PAHs, pesticides, metals and pharmaceuticals on several marine and
freshwater species of temperate and tropical areas, including mechanisms of toxicity
and detoxification.
-
Relationships between several biomarkers and behaviour endpoints.
-
Knowledge about contamination levels in NW Portuguese coast (Aveiro lagoon –
Spanish border), Minho, Lima, Cávado and Douro Rivers estuaries and Aveiro lagoon,
using integrated approaches including water quality variables, chemical analysis (e.g.
metals, PAHs, pesticides), bioaccumulation factors, biomarkers and condition indexes in
fish (eels; common goby.)
-
Knowledge on the freshwater tidal area of Minho estuary, mainly of the macrobenthic
community, population dynamics of key-species, impact of 2005 heat wave on the
community and populations resistance and resilience.
-
Identification and characterization of the non-native invasive species introduced in the
Minho and Lima rivers. Particularly attention was focused in the non-native invasive
species Corbicula fluminea, including its impact on native species and on ecosystems.
-
Functional characterization of the community of copepods of Cávado and Minho
estuaries and knowledge about resistance to pollution and microevolution due to
pollution in selected species.
-
2 PhD thesis and 7 MSc thesis (1 from another other country).
-
Advanced training in Ecotoxicology and Ecology of 15 students (3 from other countries)
and 2 researchers with PhD (2 from other countries).
132
8d. Productivity
1. Frasco, MF, Fournier, D, Carvalho, F, Guilhermino, L. 2008. Does mercury interact with the
inhibitory effect of dichlorvos on Palaemon serratus (Crustacea: Decapoda) cholinesterase?
Science of the Total Environment 404: 88-93. IF=2.579.
2. Frasco, MF, Carvalho, F, Guilhermino, L, Stojan, J, Fournier, D. 2008. Cholinesterase enzymes:
Appraisal of inhibitory mechanisms for their use as an environmental biomarker of toxicity.
Marine Environmental Research 66: 42-43. IF=2.032.
3. Lima, I, Peck, MR, Rendon-Von Osten, J, Soares, AMVM, Guilhermino, L, Rotchell, J. 2008. Ras
gene in marine mussels: a molecular level response to petrochemical exposure. Marine Pollution
Bulletin 56: 633-640. IF=2.562.
4. Nunes, B, Cardoso, MF, Carvalho, F, Guilhermino, L. 2008. The microalga Tetraselmis chuii
(Chlorophyceae) in ecotoxicology: culture conditions and growth model. Fresenius
Environmental Bulletin 17: 408-414. IF=0.463.
5. Nunes, B, Gaio, AR, Carvalho, F, Guilhermino, L. 2008. Behaviour and biomarkers of oxidative
stress in Gambusia holbrooki after acute exposure to widely used pharmaceuticals and a
detergent. Ecotoxicology and Environmental Safety 71: 341-354. IF=2.590.
6. Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2008. Uptake and depuration of anatoxin-a by
the mussel Mytillus galloprovincialis (Lamarck, 1819) under laboratory conditions. Chemosphere
72: 1235-1241. IF=3.054.
7. Quintaneiro, C, Querido, D, Monteiro, M, Guilhermino, L, Morgado, F, Soares, AMVM. 2008.
Transport and acclimation conditions for the use of an estuarine fish (Pomatoschistus microps)
in ecotoxicity bioassays: effects on enzymatic biomarkers. Chemosphere 71: 1803-1808.
IF=3.054.
Marine and Freshwater Ecosystems 18: 1063-1077. IF=1.500.
fluminea (Muller, 1774) in aquatic ecosystems: an overview. Annales de LimnologieInternational Journal of Limnology 44: 85-94. IF=0.768.
its conservation. Estuaries and Coasts 31: 1198-1107. IF=1.408.
133
Estuarine, Coastal and Shelf Science 79: 467-474. IF=2.072.
13. Sousa, R, Nogueira, AJA, Gaspar, M, Antunes, C, Guilhermino, L. 2008. Growth and extremely
high production of the non-indigenous invasive species Corbicula fluminea: possible implications
for ecosystem functioning. Estuarine, Coastal and Shelf Science 80: 289-295. IF=2.072.
Portugal. Aquatic Conservation-Marine and Freshwater Ecosystems 18: 98-110. IF=1.500.
15. Vieira, LR, Sousa, A, Frasco, MF, Lima, I, Morgado, F, Guilhermino, L. 2008. Acute effects of
benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus
microps (Teleostei, Gobiidae). Science of the Total Environment 395: 87-100. IF=2.579.
proceedings)
1. Gravato, C, Faria, M, Alves, A, Guilhermino, L. 2008. Biomonitoring studies performed with
European eel populations from the estuaries of Minho, Lima and Douro rivers (NW Portugal). In:
Kim, JY; Platt, U. (Eds). Advanced Environmental Monitoring. Springer, Dordrecht. ISBN 978-14020-6363-3 (HB), ISBN 978-1-4020-6364-0 (e-book).
2. Dias, S, Sousa, R, Lobón-Cerviá, J, Laffaille, P. 2009. The decline of diadromous fish in Western
European inland waters: main causes and consequences. In: McManus, NF, Bellinghouse, DS.
(Eds.). Fisheries: Management, Economics and Perspectives. Nova Science Publishers, Inc., New
York, USA, ISBN: 978-1-60692-303-0 (in press).
3. Guilhermino, L. 2008. Biomarkers for the assessment of effects and/or exposure to
environmental contaminants: a special reference to pesticides. Proceedings of the Summer
School Pesticides – Environment 2007. Quaderno Grifa, nº 26: 407-416. ISBN 978-88-95221-014.
proceedings)
MASTER THESES
Molecular and Cell Biology, University of Aveiro. Supervisor: AMVM Soares (Univ. Aveiro); Cosupervisors: C Gravato, M Amorim.
organisms. Master in Biology, University of Aveiro. Supervisor: L Guilhermino, Co-supervisor:
AMVM Soares (Univ. Aveiro, CESAM).
134
PhD THESIS
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: L Guilhermino, Cosupervisor: C Antunes.
-
28 gene sequences with accession numbers from FG548387 to FG548414, Liver sea bass
cDNA library, EST. (Gravato, C, Van der Ven, K, Vandenbrouck, T, De Coen, WM,
Guilhermino, L), databases: GenBank. May 2008.
-
162 gene sequences with accession numbers from GD180689 to GD180850 Liver sea
bass cDNA library, EST. (Gravato, C, Almeida, JR, Van der Ven, K, Vandenbrouck, T, De
Coen, WM, Guilhermino, L), databases: GenBank. September 2008.
-
2 devices for measuring swimming velocity and water flow resistance in fish bioassays.
-
1 device for measuring avoidance in shrimps.
Organization of more than 20 seminars and lectures with invited experts on Ecotoxicology,
Ecology, Toxicology and Environment & Human Health at CIIMAR and ICBAS.
L Guilhermino was member of the Scientific Committee of the 7th Iberian and 3rd
Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2008 and
chaired the session “ iomarkers”. Lisbon, 10-12 March 2008.
L Guilhermino was member of the Scientific Committee of the 18th Annual Meeting of SETACEurope; she also organized and chaired the session “ET07 – Linking genomics and/or biomarkers
to ecologically relevant parameters”. Warsaw, 25-29 May 2008.
C Gravato co-organized and co-chaired the session “ET08 – Toxic effects in estuarine and
brackish waters” in the 18th Annual Meeting of SETAC-Europe. Warsaw, 25-29 May 2008.
The laboratory of Ecotoxicology provides consulting and other services to industry, including
toxicity tests and support for REACH, the new European Community regulation on chemicals and
their safe use (EC 1907/2006). For example, in 2007, the laboratory performed bioassays with
fish for Spanish companies to assess the toxicity of new pesticides. In addition, the laboratory
has been also performing toxicity assays, environmental studies and consulting services
regarding the management of residuals, sediments removal and other environmental matters
for national industry, local and national Authorities (e.g. Municipalities). In addition, the
members of the group participated in meetings organized by Regional Authorities, including the
“Coordenação para o Desenvolvimento da Região Norte - CCDRN” and “Administração da Região
Hidrogr fica do Norte” for the elaboration of the Regional Agenda for the Sea, prepare calls for
QREN funded projects, and river-basin plans. In addition, L. Guilhermino has been participating
135
in projects of the Autonomous University of Campeche, Mexico, in cooperation with oil and
pesticide companies operating in the Gulf of Mexico to reduce the environmental and human
health impact of the oil extraction and transformation, and of the use of pesticides in
agriculture. Furthermore, some members of the group participated in the meetings (restricted
participation) to prepare the international workshop “EMERCHEM – Emerging Chemicals in
developing Countries” that will bring together scientists, regulators and other involved persons
and institutions from OCDE and developing countries to identify emerging pollutants of concern
in developing countries and chemical safety. It will have the scientific leadership of SETAC and
has already the support of governments, several donor countries and several world institutions
operating in these countries.
Main networks (change of students and researchers):
-
Belgium: Univ. Antwerp (De Coen).
-
Brazil: Univ. Federal Baía (da Silva).
-
Costa Rica: Univ. Nacional (Castillo).
-
Finland: Finish Environmental Institute (Lehtonen).
-
France: Univ. Paul Sabatier (Fournier), Univ. Catholique Ouest (Mouneyrac).
-
Mexico: Autonomous Univ. Campeche (Rendón von Osten), CIAD Mazatlán (BetancourtLozano, García de la Parra); Benemerita Univ Puebla (Mangas-Ramirez).
-
Norway: Univ. Oslo (Hylland), NIVA (Thomas).
-
Slovenia: Univ. Ljublajana (Stojan).
-
Spain: CSIC Torre La Sal (Navarro), CSIC Barcelona (Barata, Bayona), INIA (Tarazona),
Univs Basque Country (Cajaraville), Coruna (Mendez), Extremadura (Soler), Murcia
(García-Fernández), Valencia (TorreBlanca), Vigo (Beiras).
-
UK: Plymouth Marine Lab (Readman), Univ Sussex (Rotchell), Univ Cambridge (Aldridge).
-
USA: Univ Georgia (Byers), Western Washington Univ (Harper).
2008: L. Guilhermino was evaluator for Fondo Investigacion Científica de Argentina; She is
Associate Editor of Biomarkers, member of the Science Committee of World Council of Society
of Toxicology and Environmental Chemistry (SETAC) and SETAC Europe Council, scientific expert
of the ICES group on biological effects of pollution and is a regular reviewer of more than 20 ISI
indexed journals. R. Sousa was invited to be a member of the Editorial oard of “Water SA”
(2008-2011) and is a Council Member of Neobiota (The European Group on Biological Invasions).
The lab is involved in several networks and initiatives for international cooperation.
136
Examples of cooperative papers:
-
Domingues, I, Guilhermino, L, Soares, AMVM, Nogueira, AJA, Monaghan, KA. 2009.
Ecotoxicology and Environmental Safety 72: 450-457.
-
Lima, I, Peck, MR, Rendón-Von Osten, J, Soares, AMVM, Guilhermino, L, Rotchell, JM.
2008. Marine Pollution Bulletin 56: 633-640.
-
Frasco, MF, Fournier, D, Carvalho, F, Guilhermino, L. 2008. Science of the Total
Environment 404: 88-93.
-
Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2008. Chemosphere 72: 1235-1241.
-
Frasco, MF, Carvalho, F, Guilhermino, L, Stojan, J, Fournier, D. 2008. Marine
Environmental Research 66(1): 42-43.
The laboratory of Ecotoxicology participated in meetings to establish priorities of scientific
policy, science, research and action (e.g. management, sustainable development) at global,
European, national and regional levels in several areas such as: Environment, Marine Sciences,
Climate Changes, Chemicals use in Developing Countries.
8e. Future research
1. Objectives
-
Understanding how the presence of chemical pollution drives the evolution of
populations of key estuarine species (e.g. Carcinus maenas): by studying populations
developing in estuaries with different levels of pollution and performing laboratory
assays with individual chemicals and mixtures, relevant molecular genetics alterations
will be identified; working with selected polymorphic genes, the genetic diversity and
possible differential selection pressures in populations developing in different estuaries
will be studied.
-
Effects of pollution and natural stressors on ecosystem functioning will be investigated
using both in situ and laboratory experiments and based on previous works of the team
on several ecosystems of the NW coast of Portugal where the effects of both chemical
and natural stressors on individuals, populations and on the abiotic component of the
ecosystem is studied. For example, the effects on species competition (including
competition between native and NIS), predation, primary production and energy
transfer will be studied using approaches already in development. Particular attention
will be given to “ecosystem engineering” species, such as Corbicula fluminea.
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-
Linking “omics”, biomarkers, behaviour and intrinsic population growth rate: in the
continuation of the work that has been performed in the last year, efforts will be put in
establishing relationships between different biological organization levels to take
advantage of the new technology in understanding complex ecological and
ecotoxicological problems.
-
Development of methodologies for risk assessment of oil and chemical spills will be
standardized and validated with native species, in the scope of an already funded
international project and in the continuation of work that has been performed by the
group in previous projects (e.g. CONTROL, RISKA, EROCIPS), in the Atlantic coast of
Portugal and Galicia, with particular focus on planktonic and pelagic organisms.
-
Effects of global changes: Following the important results that have been obtained in
the estuary of the Minho river about the impact of the 2005 heat wave on molluscs
assemblages and the results from the comparison between the effects of pollution in
temperate and tropical ecosystems, efforts in investigating potential effects of climate
changes will be considerably increased. We will investigate questions such as: what are
the predicted changes on the abiotic component? How these changes will affect
different populations and the relationships among them? Will these changes modify the
toxicity of individual pollutants and their toxicological interactions in complex mixtures?
How they will influence the evolution of communities under chemical stress?
-
Tropical ecotoxicology: In Mexico, a project with endangered species (e.g. Crocodylus
moreletii) was recently initiated and will be continued. In Mexico (Campeche, Sinaloa,
Puebla), Brazil (Baia state) and Costa Rica, the research already going on about the
effects of pollution, climate changes, biodiversity conservation and sustainable
development will be continued.
2.

Current funding
189.814 EUR, FCT PTDC/MAR/71143/2006 - Molecular, biochemical and genetic variability in
estuarine populations of shore crab (Carcinus maenas) exposed to different levels of
contamination. PI: L Guimarães. 2008-2011.
90.384 EUR, EU ERA 6th FP, AMPERA (ERAC-CT2005-016165), Implementation of risk
assessment methodologies for oil and chemical spills in the European marine environment.
Partner leader: L Guilhermino. 2008-2011.

198.767 EUR, FCT PTDC/AAC-AMB/102121/2008, NISTRACKS – Processes influencing the
invasive behaviour of the non-native invasive species Corbicula fluminea (Mollusca: Bivalvia) in
estuaries - identification of genetic and environmental key factors. PI: L Guilhermino.
138
191.582 EUR, FCT PTDC/MAR/098190/2008, ECO-NIS - Ecosystem-level impacts of a nonindigenous invasive species. PI: R Sousa.
199.800 EUR, FCT PDTC/BIA-BEC/105077/2008, POPmess - The effects of Benzo(a)pyrene on
Poecilia reticulata: fish strategies to counteract and adapt to chemical stress. PI: C Gravato.
200.000 EUR, FCT PDTC/MAR/098322/2008, WESSEA – What’s the ecological relevance of
molecular and sub-cellular alterations induced by polycyclic aromatic hydrocarbons on sea bass
juveniles (Dicentrarchus labrax L.)? PI: C Gravato.
21.768 EUR, FCT PDTC/AAC-AMB/100310/2008, COOL BOX - From genes to ecosystem:
ecologically relevant effects, a linking tool box. Partner leader: C Gravato. Total budget: 193.772
EUR.
188.076 EUR, FCT PTDC/AAC-CLI/098047/2008, EPISODES - Epigenetic responses of the
microcrustacean Daphnia magna to chemical and climate changes. PI: L Guimarães.
139
Vitor Manuel Oliveira Vasconcelos
-
-
FCT
2.400 EUR, PTDC/MAR/68106/2006. PI: M Santos, A Antunes.
41.161,90 EUR, PTDC/BIA-BDE/70982/2006. PI: A Antunes.
9.654,74 EUR, POCI/BIA-BDE/61719/2004. PI: V Vasconcelos.
30.859,49 EUR, POCTI/MAR/61569/2004. PI: V Vasconcelos.
16.223,84 EUR, POCTI/CTA/46733/2002. PI: V Vasconcelos.
35.262,71 EUR, PTDC/AMB/67075/2006. PI: V Vasconcelos.
-
GRICES
GRICES/Governement de Tunisie, 2006-2008, Cyanobacteria in tunisian freshwaters. CIMAR with
INAT, Tunisia. V Vasconcelos.
GRICES/CNRST, 2006-2008, Ecotoxicology of blooms forming cyanobacteria. CIMAR with Faculté
des Sciences Semlalia, Marrocos. V Vasconcelos.
-
Other
4.000 EUR, Pre-graduation projects, 2007-2008, Survey of some pharmaceutics effects on
freshwater cyanobacteria. Univ Porto. MT Vasconcelos, V Vasconcelos.
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4.000 EUR, Pre-graduation projects, 2007-2008, Allelopathic effect of water contaminated with
cyanobacteria on the germination and growth of several plants species. Univ Porto. V
Vasconcelos.
Cooperation Portugal/México, with UNAM, México. 2008-2009, Toxic cyanobacteria in Mexican
freshwaters: Toxicological and phylogenetical analysis. V Vasconcelos.
1. Objectives
Ecotoxicological study of cyanobacteria and other potentially toxic organisms in marine,
brackish and freshwaters. Occurrence of the main species and of the main toxins, and
production of bioactive compounds with potential pharmacological interest.
Ecotoxicological assays regarding the effects of neurotoxins and hepatotoxins in different
organisms. Use of behavioral and histopathological alterations as endpoints. Studies on the
bioaccumulation of toxins and research of possible toxin vectors. Human health risk assessment
of toxin occurrence in drinking, recreational waters. Impact of contamination of toxins in water
used for agriculture.
Evolution of aquatic systems in what concerns with eutrophication processes and development
of prediction models for application by water management authorities and water treatment
plants. Development of alert systems using ecotoxicological responses of organisms in different
aquatic contamination scenarios.
Use of molecular and computational genomics/proteomics tools to characterize genes/proteins
involved in important cellular functions, notably metabolism, enzymatic activity and disease
onset in humans and other animal species, allowing an understanding of molecular adaptation
mechanisms.
Use of molecular and computational genomics/proteomics tools to characterize genes/proteins
involved in important cellular functions, notably metabolism, enzymatic activity and disease
onset in humans and other animal species, allowing an understanding of molecular adaptation
mechanisms.
Extracts of isolated marine cyanobacteria strains were tested. A high percentage of apoptotic
cells were observed for HL-60 cells when treated with cyanobacterial organic extracts. Nine
cyanobacteria strains were found to have antibiotic activity against two Gram-positive bacteria,
Clavibacter michiganensis subsp. insidiosum and Cellulomonas uda. Marine Synechocystis and
Synechococcus extracts induce apoptosis in eukaryotic cells and cause inhibition of Grampositive bacteria. Inhibitory effects on the growth of the green alga Ankistrodesmus falcatus due
to the compounds of several strains of freshwater cyanobacteria (Microcystis aeruginosa and
Planktothrix planctonica) showed differential responses to the different cyanobacteria strains.
The growth inhibition was not related to microcystins during acute exposures. Hydrophilic
141
compounds may be released to the water at very low cyanobacteria densities causing inhibition
effects while the bloom is in its early development. Massive populations of cyanobacteria may
then release more lipophilic substances that inhibit phytoplankton. The study of the effects of
cyanobacteria extracts with MC-LR on the seed germination and growth of several plants
showed that Pisum sativum is the most sensitive tested species with a 97% germination rate
reduction and Lens esculenta was the most resistant.
Microcystin (MC) contaminated mussels showed that conserved at room temperature
significantly reduced the detectable MCs in the mussels. The effects of the cooking on the MCs
availability showed that boiling does not significantly change the MCs content of the mussels.
Nevertheless, the microwave treatment significantly reduced the MCs content. On the other
side, we confirmed the low uptake and fast depuration of anatoxin-a by mussels. One day after
the beginning of depuration, the toxin could not be detected in the animals.
Genomic studies across various organisms (notably mammals) have allowed valuable insights to
understand the functional changes of various gene/proteins involved in important cellular
functions and disease onset. Such knowledge also provided reliable information to assess the
genetic diversity and conservation of various species, including charismatic and endangered
ones.
During 2008 we published a total of 14 papers in SCI journals.
8d. Productivity
1. Agüero-Chapin, G, Antunes, A, Ubeira, F; Chou, KC; González-Díaz, H. 2008. Comparative
study of topological indices of macro/supra-molecular RNA complex networks. Journal of
Chemical Information and Modeling 48(11): 2265–2277. IF=3.643.
2. Antunes, A, Troyer, JL, Roelke, ME, Pecon-Slaterry, J, Packer, C, Winterbach, C, Winterbach, H,
Hemson, G, Frank, L, Stander, P, Siefert, L, Driciru, M, Funston, PJ, Alexander, K, Prager, KC,
Mills, , Wildt, D, ush, M, O’ rien, SJ, Johnson, WE. 2008. The evolutionary dynamics of lion
Panthera leo revealed by host and viral population Genomics. PLoS Genetics 4(11): e1000251.
IF=8.883.
. Da Fonseca, R, Johnson, WE, O’ rien, SJ, Ramos, MJ, Antunes, A. 2008. The adaptive evolution
of the mammalian mitochondrial genome. BMC Genomics 9:119. IF: 4.092
4. Luo, SJ, Johnson, WE, Martenson, J, Antunes, A, Martelli, P, Uphyrkina, O, Traylor-Holzer, K,
Smith, JLD, O’ rien, SJ. 2008. Subspecies genetic assignments of worldwide captive tigers
increase conservation value of captive populations. Current Biology 18: 592-596. IF=10.777.
5. Marques, A, Antunes, A, Fernandes, PA, Ramos, MJ. 2008. Computational optimisation of
AG18051 inhibitor for amyloid-beta binding alcohol dehydrogenase (ABAD) enzyme.
International Journal of Quantum Chemistry 108: 1982-1991. IF=1.317.
142
6. Martins, R, Ramos, M, Herfindal, L, Sousa, JA, Doskeland, S, Vasconcelos, VM. 2008.
Antimicrobial and cytotoxic assessment of marine cyanobacteria extracts. Marine Drugs 6(1): 111. IF=1.200.
7. Morais, J, Augusto, M, Carvalho, AP, Vale, M., Vasconcelos, VM. 2008. Microcystins cyanobacteria hepatotoxins- bioavailability in contaminated mussels exposed to different
environmental conditions. European Food Research & Technology 227: 949-952. IF=1.622.
8. OlivaTeles, L, Pereira, E, Saker, M, Vasconcelos, V. 2008. The use of virtual models for
predicting cyanobacterial bloom dynamics. Lakes and Reservoirs: Research and Management 13:
135-143.
9. Osswald, J, Rellan, S, Gago, A, Vasconcelos, VM. 2008. Uptake and depuration of anatoxin-a
by Mytillus galloprovincialis under laboratory conditions. Chemosphere 72: 1235-1241.
IF=3.054.
10. Pereira, E, OlivaTeles, F, Vasconcelos, V. 2008. Variation of environmental parameters and
dynamics of phytoplankton in a temperate eutrophic reservoir (Torrão, Rio Tâmega, Portugal).
Fresenius Environmental Bulletin 17(12b): 2193-2199. IF=0.463.
11 Saqrane, S, El Ghazali, I, Pudra, B, Bouarab, L, Vasconcelos, V.. 2008. Effects of cyanobacteria
producing microcystins on seed germination and seedling growth of several agricultural plants.
Journal of Environmental Science and Health- Part B 43(5): 443-451. IF=0.930.
12 Troyer, JL, Vandewoude, S, Slattery, J, Mcintosh, C, Franklin, S, Antunes, A, Johnson, WE,
O’ rien, SJ. 2008. FIV cross-species transmission: an evolutionary prospective. Veterinary
Immunology and Immunopatholgy 123:159-166. IF=1.907.
13 Vasconcelos, VM, Almeida, L. 2008. Allelopathic effects of freshwater cyanobacteria species
on the green alga Ankistrodesmus falcatus. Fresenius Environmental Bulletin 17(9a): 1264-1269.
IF=0.463.
14. Osswald, J, Carvalho, AP, Claro, J, Vasconcelos, V. 2009. Effects of cyanobacterial extracts
containing anatoxin-a and rule of pure anatoxin-a on early development stages of carp.
Ecotoxicology and Environmental Safety 72: 473-478. doi:10.1016/j.ecoenv.2008.05.011.
IF=2.590.
proceedings)
proceedings)
143
MASTER THESES
Andrade, M. 2008. Avaliação dos efeitos morfológicos e ultraestruturais de microcistinas em
células Vero. Master thesis in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor:
V Vasconcelos, Co-supervisor: Paulo Pereira (INSA-Lisbon).
Fernandes, S. 2008. Estudo dos mecanismos de destoxificação de microcistinas em bivalves.
Master thesis in Applied Ecology, Faculty of Sciences, University of Porto. Supervisor: V
Vasconcelos.
Frazão, B. 2008. Ecotoxicologia de cianobactérias marinhas da região centro de Portugal. Master
thesis in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor: V Vasconcelos.
Martins, J. 2008. Estudo das comunidades de cianobactérias de uma ETAR (Águas de Gaia).
Quality Control, Faculty of Pharmacy, University of Porto. Supervisor: V Vasconcelos, Cosupervisor: Luísa Peixe (FFUP).
Silva, P. 2008. Efeitos de cilindrospermopsina na germinação e crescimento de sementes de
alface. Master thesis in Environmental Sciences and Technology, Faculty of Sciences, University
of Porto, Supervisor: V Vasconcelos.
PhD THESES
Pereira, E. 2008. Cianobactérias tóxicas em sistemas artificiais (albufeira do Torrão- Rio Tâmega
e na ETAR de Esmoriz). PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor.
V Vasconcelos.
-
1st Portuguese Conference on Cyanotoxins, 7-8 July 2008, CIIMAR, Porto, Portugal.
Chairman: V Vasconcelos.
-
III Congresso da Ordem dos Biólogos, 25-27 February 2008, Lisbon, Portugal. Member of
the Scientific Commission: V Vasconcelos.
-
CICTA 2008 - Congresso Ibérico sobre Contaminação e Toxicologia Ambiental, 10-12
March 2008, Lisbon, Portugal. Member of Scientific Commission: V Vasconcelos.
-
Internacional Conference SeaSink, 26-28 June 2008, University Fernando Pessoa, Porto,
Portugal. Member of Scientific Commission: V Vasconcelos.
Organization of conferences in 2009:
144
-
1st Iberian Conference on Cyanotoxins. FCUP, 6-7 July 2009, Porto, Portugal (V
Vasconcelos).
LEGE (V Vasconcelos) has a contract with NETIRA Ambiente, Tratamento de Águas, in order to
evaluate ecotoxicologically two new products developed to be used in the water treatment of
natural and artificial systems.
V Vasconcelos has been collaborating with several international groups resulting in publications
in peer review journals: Stephan Pflugmacher, Leibniz Inst of Freshwater Ecology-IGB, Berlin,
Germany; Stein-Oven Doskeland, Univ Bergen, Norway; Brahim Oudra, Univ Cadi Ayyad,
Marrakesh, Morocco; Ana Gago, Univ Vigo, Spain; Amel Jenhani, Inst National Agronomique de
Tunisie, Tunisia; Jordi Molgo, Lab Molecular and Cellular Neurobiology, CNRS, Paris, France;
Gustavo Montejano, Lab Phycology, National Univ Mexico, Mexico; Luis Botana, Univ Santiago
Compostela, Spain; Ana Maria Camean, Fac Pharmacy, Univ Seville, Spain.
National Contact of CYANONET - Global Network for the Hazard Management of Cyanobacterial
Blooms and Toxins in Water Resources, International Hydrology Programme of UNESCO (2005present)(V Vasconcelos).
Member of the Task Force Marine and Freshwaters Toxins of the Association of Official
Analytical Chemists - AOAC International (2007-)(V Vasconcelos).
A Antunes: Stephen J O’ rien, Warren E Johnson, Shu-Jin Luo, Jennifer Troyer, Jill PeconSlaterry, Melody E Roelke, Lab Genomic Diversity, NCI, NIH, MD, USA; Gustavo de la Riva,
Sylvester Comprehensive Cancer Center, Univ Miami, Florida, USA; Sue VandeWoude and Sam
Franklin, Colorado State Univ, USA; Craig Packer, Univ Minnesota, USA; Laurence Frank, Univ
California Berkeley, USA; Katherine C Prager, Univ California Davis, USA; David Wildt and Mitch
ush, Smithsonian’s National Zoological Park, Front Royal, USA; Kuo-Chen Chou, Gordon Life
Science Inst, San Diego, USA; Guillermin Aguero-Chapin, CBQ, Central Univ Las Villas, Santa
Clara, Cuba; Eugenio Uriarte, ITESS, Guanajuato, Mexico; Humberto Gonzalez-Diaz and Florencio
M Ubeira, Univ Santiago Compostela, Spain; Graham Hemson, Wildlife Conservation Research
Unit, Tubney, Oxon, UK; Christiaan Winterbach and Hanlie Winterbach, Tau Consultants, Maun,
Botswana; Kathy A Alexander, Dept Wildlife and National Parks, Kasane, Botswana; Philip
Stander, Ministry of Environment and Tourism, Windhoek, Namibia; Ludwig Siefert, Makerere
Univ, Kampala, Uganda; Margaret Driciru, Uganda Wildlife Authority, Kampala, Uganda; Paul J
Funston, Tshwane Univ Technology, Pretoria, South Africa; Gus Mills, Univ Pretoria, South
Africa; Paolo Martelli Ocean Park, Aberdeen, Hong Kong; Olga Uphyrkina, Inst Biology and Soil
Sciences, Vladivostok, Russia.
145
LEGE has been responsible for the analysis of water and counseling to many public health
departments in what concerns the environmental and human health risk manangent associated
with toxic cyanobacteria and other phytoplankton.
LEGE has been working in a national project coordinated by INAG (Portuguese Water Institute,
Ministry of Environment) in the Water Framework Directive of Costal Waters. Responsible for
the phytoplankton analysis of northern estuaries.
V. Vasconcelos joined the team of Quaternaire in order to elaborate the Management Plan for
the Alto Rabagão Reservoir (2008-2009) being responsible for the Waster quality chapter.
V Vasconcelos is also working with INAG in order to elaborate a standard for phytoplankton
analysis in freshwaters.
V. Vasconcelos, as the scientific Coordinator of CMIA Vila do Conde (Interpretation and
Monitoring Center of Vila do Conde) has directed the study of the physico-chemical and
microbiological quality of the beaches of Vila do Conde area. A Report on the Water Quality of
those beaches during 2008 was published and distributed to Public Health Authorities,
Municipality of Vila do Conde and CIMAR.
8e. Future research
1. Objectives
-
Development of early warning methods for the detection and quantification of
cyanobacteria and cyanotoxins using molecular approaches (RT PCR) and
ecotoxicological approaches. Search for new bioactive compounds including new toxins
from marine environments using marine invertebrates as sources. Occurrence,
transmission and risk analysis of tetrodotoxin. Risk analysis of the BMMA in marine and
freshwater environments including development of new analytical techniques, HPLC
and Capilary Electrophoresis. Study of action of known and unknown toxins from
cyanobacteria using a proteomic approach.
-
Evolutionary genomics/proteomics studies of cellular protein-coding genes operating in
venom production, detoxification, development, immune system, and genetic disease
onset; and the inference of the comparative genomic principles from various organisms
(from marine microorganisms to mammals) that participate in these processes. Such
patterns of genomic variation across organisms are of critical value in interpreting the
structural dynamics and the functional diversifications that occurred in organismal
genomes over millions of years of evolution. Such knowledge also provides reliable
insight for biodiversity studies of various species, from marine microorganisms to
mammals.
146
2.

Current funding
188.980 EUR, PTDC/MAR/68106/2006, 2008-2010. M Santos, A Antunes.
140.818 EUR, PTDC/AMB/67075/2006, 2008-2010. V Vasconcelos.
148.000 EUR, PTDC/BIA-BDE/70982/2006, 2008-2010. A Antunes.
138.033,43 EUR, INTERREG IV Atlantic Area, ATLANTOX, 2009-2011.

Pending funding
FCT PTDC/AAC-AMB/104983/2008, A. Antunes.
FCT PTDC/MAR/099642/2008, V. Vasconcelos.
FCT PTDC/MAR/102258/2008, M. Martins.
FCT PTDC/MAR/104982/2008, A. Antunes.
FCT PTDC/BIA-MIC/102324/2008, V. Vasconcelos.
FCT PTDC/BIA-BEC/099344/2008, A. Antunes.
INTERREG IV Atlantic Area, ShareBiotech, A. Antunes.
147
Maria Clara Semedo da Silva Costa
-
-
FCT projects funding in 2008:
9.014,86 EUR, FCT PPCDT/AMB/58512/2004, ECOTEC - Eco-technologies based on the use of
sulphate-reducing bacteria and their application for the treatment of acid mine drainage. In
collaboration with Instituto Nacional de Engenharia Tecnologia e Inovação (INETI).
882,04 EUR, FCT POCI/AMB/60257/2004, GERMINARE - Geochemistry and remediation of
abandoned mines and surrounding areas. In collaboration with the University of Évora.
1. Objectives
The central aim of Environmental Technologies group is to contribute, with fundamental and
applied research, to the better knowledge and development of sustainable technologies for the
decontamination of metal contaminated waters or liquid effluents.
Objectives of bioremediation studies
a) The central objectives of this group included the main purpose of ECOTEC and GERMINARE
projects which was to establish a simple, natural and low cost eco-technology, using sulphatereducing bacteria (SRB), at bench scale, for the treatment of acid mine drainage (AMD) waters.
2008 was the last year of ECOTEC and GERMINARE projects. Thus, after the studies previously
mentioned on the report of 2006* to 2007, during 2008 the optimization of the experimental
parameters of the bioremediation systems previously established (eg. increase in contaminants
148
concentration and improvement of the hydraulic retention time) was an important concern. To
test the bioremediation systems using real AMD was also a main objective.
b) The investigation of the effect of uranium (VI) in the structure of bacterial communities used
in bio-removal studies were a relevant objective of this group. In fact the dynamics of microbial
populations with ability for uranium removal are poorly understood and little is know about the
modifications in the community structure and composition in response to changes in growth
conditions, such as the addition of metal ions. In addition, to establish a relation between
uranium (VI) and chromium (VI) bio-removal and the microbial community structure was also an
important topic of research. These objectives are included in the PhD work of Mónica Martins.
Objectives of liquid-liquid extraction studies
The main aim is now to investigate the potentialities of amide derivatives (eg. malomanides,
succinamides) for the liquid-liquid extraction of platinum group metals.
This line is a natural development of previous investigation devoted to the extraction of iron (III)
from hydrometallurgical chloride solutions.
* The first year of Environmental Technologies group.
a) ECOTEC and GERMINARE projects: The optimization of the bioremediation systems previously
developed (see Patents/Prototypes) was the main achievement. That optimization allowed the
production of water suitable for irrigation (according to the Portuguese legislation) from real
AMD (from the mines of S. Domingos and Tinoca), even when the concentration of sulphate
increase as a result of possible seasonal variations (publication submitted).
The proof that wine wastes were effective as carbon source when used in one of the
bioremediation systems developed (Costa et al. Chemosphere, in press), was a very important
achievement considering that the economical viability of these processes is often compromise
by the cost of the substrate.
b) The investigation performed showed that the structure of bacterial communities, and
particularly the SRB structure, is greatly affected by the presence of uranium (VI). This fact, not
usually taken into account, is determinant to understand the behavior of the communities (eg.
in terms of sulphate reduction and uranium removal), and thus it represents an important
finding. The microbial structure of the bacterial communities with ability for uranium (VI) and
chromium (VI) bio-removal was compared. All bacteria were modified when uranium (VI) was
replaced by chromium (VI). The absence of sulphate did not affect the community responsible
for uranium removal. The community structure maintains stable, even when uranium
concentration was double increased. However, the community responsible for chromium
removal is affected by the presence of sulphate. (Two publications in preparation).
Liquid-liquid extraction studies
The potentialities of succinamide molecules for the liquid-liquid extraction of platinum from
hydrochloric acid media were proved, in preliminary studies, and were compared with those of
149
malonamides, previously investigated for the solvent extraction of iron from hydrochloric acid
solutions. In spite of this very promising result, the lack of financial support (not allows having a
researcher devoted to this work to pursue this line of investigation) compromises its
development.
8d. Productivity
1. Costa, MC, Martins, M, Jesus, C, Duarte, JC. 2008. Treatment of acid mine drainage by
sulphate-reducing bacteria using low cost matrices. Water Air and Soil Pollution 189: 149-162.
IF=1.398.
2. Hamidov, A, Khaydarova, V, Sharipova, S, Costa, MC, Beltrão, J. 2008. Environmentally useful
technique - Portulaca oleracea golden purslane as a salt removal species. WSEAS Transactions
on Environment and Development 7(3): 117-122.
3. Martins, M, Faleiro, ML, Barros, RJ, Veríssimo, AR, Barreiros, MA, Costa, MC. 2008.
Characterization and activity studies of highly heavy metal resistant sulphate-reducing bacteria
to be used in acid mine drainage treatment. Journal of Hazardous Materials,
doi:10.1016/j.jhazmat.2008.11.088. IF=2.975.
4. Martins, M, Faleiro, L, Barros, RJ, Veríssimo, AR, Costa, MC. 2008. Biological sulphate
reduction using food industry wastes as carbon sources. Biodegradation, doi:10.1007/s10532008-9245-8. IF=2.055.
5. Barros, RJ, Jesus, C, Martins, M, Costa, MC. 2008. Marble stone processing powder residue as
chemical adjuvant for the biologic treatment of acid mine drainage. Process Biochemistry,
doi:10.1016/j.procbio.2008.12.013. IF=2.144.
6. Costa, MC, Santos, ES, Barros, RJ, Pires, C, Martins, M. Wine wastes as carbon source for
biological
treatment
of
acid
mine
drainage.
Chemosphere,
doi:10.1016/j.chemosphere.2008.12.062. IF=3.054.
proceedings)
1. Paiva, AP, Malik, P, Rosa, AM, Castro, C, Costa, MC. 2008. Extraction of metal ions from
chloride media: can malonamide derivatives be useful?. Proceedings of the International Solvent
Extraction Conference - ISEC 2008 (Ed. BA Moyer), Vol. 2, pp. 1439-1444, Tucson, Arizona, USA.
2. Martins, M, Santos, ES, Barros, RJ, Costa, MC. Treatment of acid mine drainage with sulphatereducing bacteria using a two-stage bioremediation process. Proceedings of the 10th
International Mine Water Association Congress, IMWA`08, pp. 297-300, Carlsbad, Czech
Republic.
150
proceedings)
MASTER THESES
Hamidov, A. 2008. Salt removing species: Fitoremediation technique for Uzbekistan. Erasmus
Mundus European Joint Master, University of Algarve. Supervisor: Costa MC.
Considering the important applicability of wine wastes as carbon source for sulphate-reducing
bacteria a national patent was made:
-
Costa, MC, Martins, M, Barros, R. 2008. Uso de resíduos vinícolas como fonte de
carbono e de energia para bactérias sulfato-redutoras. Portuguese Patent nº 104182.
-
Two laboratorial protopypes were developed and optimised in the framework of
ECOTEC project, for the treatment of AMD.
Collaboration with Dr. Valentina Khaydarova from Water Resources Department from the Uzbek
State Uzgipromeliovodhoz Institute in Tashkent, Ubzequistan and with Dr. S. Sharipova from the
Faculty of Hydro-amelioration of the Tashkent Institute of Irrigation and Melioration
Ubzequistan, in fitoremediation studies using food crops for the removal of salt from the
contaminated soils of Ubzequistan. That collaboration was in the framework of an Erasmus
Mundus European Joint Master, in which the University of Algarve participated. As a result of
that collaboration a master thesis was accomplished, as well as one publication (Hamidov, A et
al. 2008. WSEAS Transactions on Environment and Development 7(3): 117-122).
Our group also collaborated with the pos-doc student Dr. Ponma Malik from India for the
development of liquid-liquid extraction studies aiming the recovery of metal ions from chloride
hydrometallurgical and waste solutions. One publication (Hamidov, A et al. 2008. WSEAS
Transactions on Environment and Development 7(3): 117-122.) resulted from that collaboration.
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During 2008 collaboration with the group of Prof. Jose Miguel Nieto, from Huelva University,
was initiated in view of a future application to an international program to allow the
demonstration on situ of the bioremediation technology developed during ECOTEC project.
8e. Future research
1. Objectives
General objectives
Taking into account the diversity and the high abundance of microorganisms in the natural
environment it is our aim to identify and characterize microbial strains or communities
especially with high metals resistance and with ability for metals removal, and thus with
potentialities for bioremediation. In that context this group is now interested in extending its
research to the exploration of environmental samples from extreme environments, eventually
more adapted to high metals concentration and to low pHs. Understanding and exploring the
potential of microbial-metal interactions have gained recent interest due to their importance in
various high end biotechnological applications, like microbe mediated nanomaterials synthesis,
and that is therefore a main concern of the “Environmental Technologies” group. Investigate
eventual modifications in bacterial community structures in response to changes in growth
conditions, such as in cases where metal ions are added, is also intended.
Specific objectives
Following the studies initiated with ECOTEC project:
-
Investigate the dynamic of the bacterial population during the bioremediation process
developed in the framework of “ECOTEC” project for the treatment of AMD by SRB.
-
Investigate the production of biological nanosulphides and nano composites, preferably
as a result of the bioremediation process, instead of waste sulphide metals. This will be
the subject of the PhD work of João Pinto da Costa.
In the framework of Mónica Martins PhD:
-
Investigate the mechanism of uranium (VI) removal by anaerobic consortia previously
selected.
-
Identification of the enzymes responsible for eventual uranium (VI) reduction.
-
To extend the studies carried out with the anaerobic consortia to the removal of
aluminium and eventually platinum. Besides the screening of anaerobic consortia from
environmental samples with ability for the removal of those metals, metals-bacteria
152
interactions will be also investigated to understand the mechanism of metals removal
and to assess biotechnological potentialities.
In the framework of Maria Alexandrino Fernandes post-doc:
-
Bacterial diversity studies of environmental samples from extreme environments (eg.
sediments from marine and terrestrial hot springs, samples from acidic and metals
contaminated areas).
-
Investigate the resistance of the bacterial consortia from extreme environments to low
pHs and to metals. Evaluate the ability of those samples for metals removal (eg. Fe, Cu,
Zn and Pt) especially at low pHs.
2.

Current funding

FCT PTDC/MAR/102177/2008, BIOREMVENT - Identification and bioremediation potential of
anaerobic bacteria from hydrothermal vent sediments. PI: Maria Clara Costa, CCMAR as
Principal contractor.
FCT PTDC/AAC-AMB/098599/2008, BIONANOMINE - Bio-synthesis of nanosized semiconductors
using mine wastes as materials sources. PI: Maria Clara Costa, CCMAR as Principal contractor.
The members of Environmental Technologies group (Maria Clara Costa and Mónica Martins)
also participate in the following research projects:
FCT PTDC/BIA-MIC/101319/2008, YEASTREME - Metal resistance mechanisms in extremophilic
yeasts: functional and evolutive aspects. Coordinated by Dr. Mário João Gadanho, Centro de
Biodiversidade, Genómica Integrativa e Funcional (BioFIG) as Principal contractor.
FCT PTDC/AGR-AAM/102664/2008, Is Plantago almogravensis an aluminium hyperaccumulator
plant? Elucidation of the tolerance mechanism using micropropagated plants. Coordinated by
Prof. Anabela Romano, University of the Algarve as Principal contractor.
153
Maria Armanda Reis Henriques
-
-
National funding
8.473,62 EUR, FCT POCI/MAR/59462/2004, The molecular mechanisms of sexual differentiation
in gastropode and the role of vertebrate - like steroid hormones. PI: Filipe Castro.
33.085,08 EUR, FCT POCI/MAR/59094/2004, SEAQUA - Quality of seabass and seabream;
accumulation of organochlorine compounds and heavy metals and biomarkers of exposition. PI:
MA Henriques.
4.573,78 EUR, FCT POCI/MAR/60895/2004, Effects of complex mixtures of antagonistic
endocrine disrupting chemicals in two fish species with different life histories. PI at CIIMAR:
Miguel Santos.
2.490,00 EUR, FCT PTDC/MAR/68106/2006, The modulations of retinoic acid signalling pathways
by environmental pollutants in teleosts. PI: Miguel Santos.
Miguel Santos.
2.490,00 EUR, FCT PTDC/MAR/68106/2006, The modulations of retinoic acid signalling pathways
by environmental pollutants in teleosts. PI: Miguel Santos.
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1. Objectives
The research of LETOX in 2008 focused in several areas: In the scope of endocrine disruption
(ED) we pursed several complementary aims. In the aquatic environment endocrine disrupting
chemicals (EDCs) do not exist alone. Therefore, single ecotoxicological evaluations may not
reflect the multitude of antagonistic or synergistic stimulus that wildlife animals may face.
Hence, under controlled laboratory conditions we aimed at evaluating the effects and the
molecular and biochemical mechanisms of disruption of fish and marine invertebrate’s exposure
to EDCs. Studies aimed at evaluating both single and complex mixtures of EDCs with antagonist
mode of action (estrogenic and androgenic chemicals). The new research area concerning
pharmaceuticals in the aquatic environment started. Among the quantity of pharmaceuticals
sold, psychopharmaca comprise a mayor group in terms of sales – particularly in Portugal. Since
they specifically target neurotransmitters in the brain, severe impact on vital functions such as
the endocrine regulation has to be assumed at minute concentrations. In an environmental
monitoring study, prevailing psychopharmaceuticals in a hot spot estuary (Douro) are identified
and subsequently considered as model substances to reveal their impact on the main hormone
systems (reproduction, osmoregulation, growth and development) in fish. Assessing the
neuroendocrine disruption will not just provide sophisticated data for environmental risk
assessment but allow for new insights on pituitary neuroendocrinology and the interlinkage of
hormone systems, contributing to a better understanding of fish endocrinology. In parallel, we
continued our research on the mechanisms of detoxification of organic contaminants including a
new topic of research on efflux membrane transporters in fish, the multidrug resistance pump
P-glycoprotein (Pgp) and the multridrug resistance-associated proteins (MRPs) involved in the
multixenobiotic resistance (MXR). We intended to establish the impact of fish feeds on
aquaculture production systems on the sea food quality and safety and the contribution to the
control of flesh quality and oxidative stability, together with health benefits for consumer.
In the scope of ED, we have continued an innovative approach which aims the understanding of
the effects of antagonistic EDCs under complex mixtures, both in invertebrates and fish. In a
step further to understand the effects of more complex mixture of chemicals with an antagonist
mode o action, we have demonstrated, for the first time for invertebrate molluscs, that the
masculinising effects of one of the main environmental xenoandrogens (TBT) can be
ameliorated by concomitant exposure to estrogenic chemicals in gastropods. This highlights the
challenge that we face when trying to integrate all the mechanistic findings in hazard
assessment procedures. We have also isolated several nuclear receptors in invertebrates, and,
at the moment, we are testing the functionality of such signalling pathways, and whether they
are prone to disruption by several environmental chemicals. In the scope of the development
and validation of biomarkers for environmental monitoring, we have focused our attention in
the use of the blenny Lipophrys pholis in pollution monitoring, particularly towards Polycyclic
Aromatic Hidrocarbons (PAHs) pollution. Our data clearly demonstrate that this species has a
high potential for pollution monitoring in the northwestern European marine ecosystems.
Additionally, together with Spanish colleagues, we have investigated the biochemical effects of
the water-accommodated fraction of the “Prestige” fuel oil in juvenile sole (Solea senegalensis).
We established the impact of fish feeds and water on aquaculture production of two species sea
155
bass and white sea bream by the evaluation the presence of persistent organic pollutants (POPs)
and the more representative metals (Cd, Hg, Pb and As). We have now cloned and sequenced
several ABC transporter genes in two important model species, the European seabass
(Dicentrarchus labrax) and nile tilapia (Oreochromis niloticus). Moreover, MXR activity assays in
seabass primary cultured hepatocytes have been implemented and can become a useful tool, in
cooperation with the other biotransformation enzymes (phase I and II), to screen pollutants
effects, at the detoxification level, in a marine species.
8d. Productivity
1. Antunes, P, Hendriks, AJ, Huijbregts, MAJ, Gil, O, Reis-Henriques, MA. 2008. Organ-specific
accumulation and elimination patterns of PCBs in adult seabass (Dicentrarchus labrax). Science
of Total Environment 407: 204-210. IF=2.579.
2. Batista, FM, Ben-Hamadou, R, Fonseca, VG, Taris, N, Ruano, F, Reis-Henriques, MA, Boudry, P.
2008. Comparative study of shell shape and muscle scar pigmentation in the closely related
cupped oysters Crassostrea angulata, C. gigas and their reciprocal hybrids. Aquatic Living
Resources 21: 31-38. IF=0.667.
3. Cunha, SR, Gonçalves, R, Silva, SR, Correia, AD. 2008. An automated marine biomonitoring
4. Fernandes, C, Fontainhas-Fernandes, A., Ferreira, M, Salgado, MA. 2008. Oxidative stress
Archives of Environmental Contamination and Toxicology 55: 262-269. IF=1.864.
5. Ferreira, M, Antunes, P, Costa, J., Amado, J, Gil, O, Pousão-Ferreira, P, Vale, C, Reis-Henriques,
MA. 2008. Organochlorine bioaccumulation and biomarkers levels in culture and wild white
seabream (Diplodus sargus). Chemosphere 73: 1669-1674. IF=3.054.
6. Ferreira, M, Caetano, M, Costa, J, Pousão-Ferreira, P, Vale, C, Reis-Henriques, MA. 2008.
Metal accumulation and oxidative stress responses in, cultured and wild, white seabream from
Northest Atlantic. Science Total Environment 407: 638-646. IF=2.579.
7. Gonçalves, R, Scholze, M, Ferreira, AM, Martins, M, Correia, AD. 2008. The joint effect of
polycyclic aromatic hydrocarbons on fish behavior. Environmental Research 108: 205-213.
IF=3.038.
8. Lima, D, Santos, MM, Ferreira, AM, Micaelo, C, Reis-Henriques, MA. 2008. The use of the
shanny Lipophrys pholis for pollution monitoring: A new sentinel species for the northwestern
European marine ecosystems. Environment International 34 (1): 94-101. IF=3.516.
156
gastropod Nucella lapillus. Comparative Biochemistry and Physiology, Part C 148 (1): 87-93.
IF=2.530.
10. Solé, M, Lima, D, Reis-Henriques, MA, Santos, MM. 2008. Stress biomarkers in juvenile
Senegal sole, Solea senegalensis, exposed to the water-accommodated fraction of the "Prestige"
fuel oil. Bulletin of Environmental Contamination and Toxicology 80: 19-23. IF=0.609.
11. Solé, M, Lobera, G, Lima, D, Reis-Henriques, MA, Santos, MM. 2008. Estearases activities and
lipid peroxidation levels in muscle tissue of the shanny Lipophrys pholis along several sites from
the Portuguese Coast. Marine Pollution Bulletin 56 (5): 999-1007. IF=2.562.
proceedings)
1. Antunes, P, Gil, O, Reis-Henriques, MA. 2008. Accumulation of PCB metabolites in plasma of
seabass (Dicentrarchus labrax). DIOXIN 2008 - 28th. International Symposium on Halogenated
Environmental Organic Pollutants (POPs), 17-22 August. Birmingham, England UK.
Organohalogen Compouds 70: 1985-1988.
2. Antunes, P, Gil, O, Hendriks, AJ, Huijbregts, MAJ, Reis-Henriques, MA. 2008. Comparing
elimination pattern of PCBs in juvenile and adult seabass (Dicentrarchus labrax). DIOXIN 2008 28th. International Symposium on Halogenated Environmental Organic Pollutants (POPs), 17-22
August. Birmingham, England UK. Organohalogen Compounds 70: 1989-1992.
proceedings)
PhD THESES
and reproductive cycle. PhD thesis. Supervisor: Odete Gil (INIAP/IPIMAR), Co-supervisor: MA
Reis-Henriques.
157
Spain
-
Institut de Ciences del Mar – CSIC, Dr. Montserrat Sóle; the collaboration deals with the
effects of oil pollution in fish and invertebrates.
-
Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR).
Germany
-
Federal Institute of Hydrology (Koblenz), Dr. Thomas Ternes, Dr. Guido Fink; Chemical
analysis of pharmaceutical residues in the aquatic environment.
-
Institute of Freshwater Ecology and Inland Fisheries (Berlin), Professor Dr. Werner Kloas,
Dr. Ilka Lutz; Expression analysis of single cells (Laseer dissection microscope, qPCR), two
ongoing projects (“Parasites as endocrine disruptors”, “Reproductive physiology of
Sander”).
Italy
-
ENEA Centro Ricerche Casaccia, Dr. Roberto Morabito and Dr. Paolo Massanisso; we
seek to understand the effects of pollutants with endocrine disrupting properties, in
sexual development and reproduction of fish.
-
Dipartimento di Scienze Ambientali, Università Ca’ Foscari Venezia.
Tunisia
-
University of Bizerte, Laboratory of Environment Bio-monitoring, Department of
Biology; the collaboration focus on the effects of EDCs in molluscs.
Japan
-
Osaka University, Department of Toxicology, Prof. Junichi Nishikawa; Together with this
group, we try to better understand the effects of EDCs in invertebrates, through
modulation of nuclear receptors. This collaboration has already resulted in one
publication in a reputable international journal.
Croatia
-
158
Rudjer Boskovic Institute (Zagreb), Laboratory for Molecular Ecotoxicology, Dr. Tvrtko
Smital; purpose to increase the knowledge on the detoxification mechanisms in aquatic
species, by studying phase 0 and III.
India
-
National Institute of Oceanography, Dona Paula, Goa; To evaluate the impact of the
more representative organic pollutants in some species of fish living in estuaries and
coastal zones.
France
-
Conseil Régional d’Aquaculture, Conseil Régional de retagne, Conseil Régional des Pays
de la Loire, Conseil Régional de Poitou Charentis.
United Kingdom
-
Department of Biological Sciences, Brunel University, Centre for Toxicology, The School
of Pharmacy, University of London, Devon Country Council, Dorset Country Council,
Pembrokeshire County Council, Environmental and Heritage Services, Devon Wildlife
Trust.
8e. Future research
1. Objectives
1. The research on the MXR mechanisms will be continued focusing on evaluating interaction of
pollutants with the efflux transporters, and in general the detoxifications mechanism in aquatic
species. Some chemicals can compromise this defence mechanism by inhibiting the transporters
in such a way that chemicals normally excluded from the cells can now exert their toxic effects,
sensitising the cell – hence the term chemosensitization. In vitro models, as primary cultured
hepatocytes from marine and fresh water species, will be used to address the
chemosensitization problem, in the presence of pollutant mixtures. Moreover, in the near future
we will also assess the role of these important efflux transporters in chemically induced
neoplasia in the model species zebrafish.
2. In the field of ED, we will continue the research involving the study of chemical mixtures with
antagonistic effects. We will focus in changes at different levels of biological organization, i.e.,
from changes in gene expression up to alterations on population dynamics. We have shown the
importance of the modulation of the retinoic acid signalling pathways in the development of ED
in gastropods. We will continue this line of research including the impact in fish. One of the
innovative aspects is the study of disruption mechanisms in phylogenetically distinct groups thus
taking into account an evolutionary perspective.
3. Since psychopharmaceuticals (PP) target the secretion of the principal pituitary hormones in
humans, it has to be expected that ecotoxicological impact affects several endocrine systems.
Due to the relevance of salinity as major ecological factor in the estuaries and coastal zone, PP-
159
induced neuroendocrine disruption is addressed in an experimental approach with regard to
osmotic challenge with special focus on the main hormones involved in freshwater saltwater
adaptation. Parameters evaluated to characterise the adverse effects incorporate
molecular/physiologic (gene expression, plasma hormones), the organ/tissue-specific (gross
morphology, histology) as well as the organisms (growth, reproduction, osmotic homeostasis)
level. Impact on four endocrine systems (adrenal, growth, reproduction and osmoregulation)
will be addressed and characterized as chronic change in gene expression (real-time RT-PCR for
gene expression analysis) of the pituitary hormones. The resulting impact on the regulated
organs (e.g. ovary) is evaluated by plasma hormone concentration and gene expression of
selected markers (e.g. vitellogenin mRNA expression in response to estradiol). In addition,
simple behaviour tests are applied to access swimming performance and courtship behaviour.
4. In the scope of ARCOPOL project work will be developed in two main areas: methodology for
environmental damage assessment (review of impacts of past oil and HNS incidents on marine
ecosystems; review of ecological risk assessment methodologies for oil and HNS pollution
incidents and standardize monitoring procedures for the assessment of environmental damage
and restoration after spill oil or HNS. And the second area establishing methodologies for
economic damage assessment of marine marketable resources (identify major gaps in the build
up of economic damage information of marine marketable resources for the support of claims;
decontamination kinetics in marine marketable resources after oil and HNS spills; standardize
procedures for management of contaminated marine marketable resources).
2.

Current funding
24.500 R, FCT Pluriannual funding.
32.280,00 EUR, FCT POCI/MAR/59462/2004, The molecular mechanisms of sexual
differentiation in gastropode and the role of vertebrate - like steroid hormones. PI: Filipe Castro.
01/10/2005-01/10/2008.
49.944,00 EUR, FCT POCI/MAR/59094/2004, SEAQUA - Quality of seabass and seabream;
accumulation of organochlorine compounds and heavy metals and biomarkers of exposition. PI:
MA Henriques. 2/11/2005-30/11/2008.
Miguel Santos. 1/10/2005-30/03/2009.
71.000,00 EUR, FCT PTDC/MAR/68106/2006, The modulations of retinoic acid signalling
pathways by environmental pollutants in teleosts. PI: Miguel Santos. 1/09/2008-30/08/2011.
265.120,00 EUR, INTERREG IV, Atlantic Area, ARCOPOL - The Atlantic Regions’ COastal POLlution
Response. Partner Scientific Leader: Maria Armanda Henriques, 01/01/2009-31/12/2011.
160

Pending funding
186.875,00 EUR, FCT PTDC/MAR/098791/2008, The role of ABC transporter proteins in
chemically indiced haepatic cancer in zebrafish (Danio rerio), 2010-2012. PI: Marta Ferreira.
199.960,00 EUR, FCT PTDC/BIA-BEC/105622/2008, The evolution of retinoid and thyroid
hormone signalling pathways, and their modulation by endocrine disruption chemicals, 20102012. PI: Miguel Santos.
156.030,00 EUR, FCT PTDC/MAR/100837/2008, Psychopharmaceuticals as neuro-endocrine
disruptors in fish, 2010-2012. PI: Sven Würtz.
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Evolution, Development and Gene Expression (EDGE)
Molecular Biology of Marine Organisms (MBMO), now consolidated as group EDGE (Evolution,
Development and Gene Expression)
Maria Leonor Quintais Cancela Fonseca
MBMO research activities have been funded through several national and international sources:
-
Prizes
50.000 EUR, Programa na Fronteira das Ciências da Vida. Prize awarded by the Calouste
Gulbenkian Foundation to Daniel M. Tiago (PI), Vincent Laizé, Manuel Santos and M. Leonor
Cancela. Prize awarded in 2008, money transferred in 2009.
50.000 EUR, CERATONIA 2007. Prize awarded by Caixa Geral de Depósitos/UALG to Pedro
Rodrigues. Prize awarded in 2007, money transferred in 2008.
-
Institutional (CCMAR)
-
International (awarded or ongoing in 2008)
16.000 EUR, SEACASE EU-FP6-2005-SSP5A.
46.488 EUR, MGE EU-FP6-2004-GOCE-CT-505403.
13.971.049 EUR/CCMAR: 2.668.249 EUR, ASSEMBLE EU-FP7-2008-227799.
-
National (awarded or ongoing in 2008)
60.000 EUR, FCT PTDC/MAR/71685/2006, HYDRAA, 04/2008-03/2011.
83.450 EUR, FCT PTDC/MAR/70855/2006, FATTYBONE, 08/2008-07/2011.
162
161.115 EUR, FCT PTDC/CVT/72083/2006, ISOPERK, 08/2007-07/2010.
60.000 EUR, FCT PTDC/MAR/70858/2006, TEXBREAM, 06/2008-05/2011.
10.000 EUR, FCT PTDC/MAR/64533/2006, CRYOSPERM, 08/2008-07/2011.
-
Collaborative actions
3.000 EUR, PESSOA-DeepBone.
COST Action FA0801-LARVANET (budget: not applicable).
-
Industrial contracts
NOVOZYMES, Denmark, 2008, 13.000 EUR. RNA extraction and cDNA library construction from
marine invertebrates.
1. Objectives
I. Molecular determinants of tissue mineralization
-
Develop fish & amphibians in vitro systems to analyse mineralization-related pathways,
gene expression & regulation, and test mineralogenic effects of pharmaceutical drugs &
environmental pollutants.
-
Identify orthologs of mammalian bone Gla-proteins and other bone- & cartilage-related
proteins in fish, study their spatio-temporal expression & transcriptional regulation to
bring insight into their function & evolution.
-
Identify human orthologs of novel genes identified in fish, identify their function,
regulation and relevance to human pathologies.
-
Determine microRNAs involved in fish skeletogenesis and in vitro mineralization and
identify target gene(s).
-
Identify genes, proteins & regulatory pathways involved in normal and abnormal
skeletal development & mineralization using high-throughput genomic and proteomic
tools.
-
Implement zebrafish as a model system to study human pathologies involving abnormal
calcification and identify marker genes and molecular mechanisms involved.
-
Establish purification methodologies and use proteomic approaches to identify new
proteins involved in calcium metabolism. Establish relevance for human health and
disease.
163
II. Perkinsus-bivalve interactions
-
Determine Perkinsus infection prevalence in bivalves along Portuguese coast by
performing an extended & continued survey. Identify species permissive/nonpermissive to the Perkinsus parasite.
-
Identify environmental parameters affecting Perkinsus survival/proliferation/infection.
-
Develop molecular diagnostic tools to evaluate bivalve infection by Perkinsus.
-
Develop integrated high-throughput genomic tools to identify Perkinsus genes &
metabolic pathways by functional genomics.
-
Analyse Perkinsus-clam interactions using subtractive hybridization & differential display
approaches to unveil genes & metabolic pathways involved in parasite infection.
III. Population genetics
-
Development of molecular tools suitable for paternity/sexing and genotyping wild
populations for conservation & management purposes, including Portuguese
populations of onelli’s eagle and otters, and economically relevant marine crustaceans
and fish, as well as for implementation to aquaculture species of interest.
I. Molecular determinants of tissue mineralization
-
Development of bone-derived cell lines from a variety of marine & freshwater fish and
amphibian.
-
Development of the fish cell line database (FICELdb).
-
Purification & cloning of OC& MGP in a variety of fish (including an ancestral OC in
sturgeon). Evidence for 2 isoforms in fish vs 1 in mammals.
-
Characterization of the evolutionary relationship of Gla proteins and identification of
domains/residues essential for function.
-
Cloning of mineralization-related gene promoters from fish/amphibians, analysis of
promoter activity and identification of transcription factor binding sites.
-
Identification of signaling pathways regulating in vitro mineralization of fish bonederived cell lines.
-
Proteomic analysis of oyster nacre & identification of osteoinductive proteins.
-
Implementation of zebrafish rearing and microinjection facilities.
-
Development of new techniques for DNA delivery into embryos (lipid-based and lasermediated delivery) & bone-derived cells.
164
-
Establishment of a comparative proteomics platform for fish protein identification.
-
Identification of proteins related to bone deformities in fish by 2DE techniques.
II. Perkinsus-bivalve interactions
-
Development of a clonal culture of Perkinsus olseni (PO) allowed in vitro testing of drugs
(e.g. antimalarial) and ex vivo clam infection to identify environmental conditions
affecting parasite proliferation/survival.
-
Development of a PCR-ELISA-based assay to determine Perkinsus levels & species.
-
Extended survey of PO prevalence along Portuguese coast. Infection linked to water
temperature/salinity/pollution, iron-dependent, and inhibited by glyphosate &
pyrimethamine.
-
Identification through functional genomics of target metabolic pathways (e.g. shikimate,
folate) to control Perkinsus infection in hatcheries.
III. Population genetics
Development & optimization of:
-
Microsatellites for onelli’s eagle towards conservation studies.
-
Techniques to preserve/extract DNA from faecal samples suitable to estimate effective
population size (offered as external service).
-
Microsatellites for routine use in seabream and sole.
-
Paternity/sexing and genetic diversity analyses to determine differential contribution
from brooders in specific populations (management and aquaculture).
8d. Productivity
1. Conceição, N, Laizé, V, Simões, B, Pombinho, AR, Cancela, ML. 2008. Retinoic acid is a
negative regulator of matrix Gla protein gene expression in teleost fish Sparus aurata.
Biochimica Biophysica Acta 1779: 28-39. IF=2.282.
2. Elandalloussi, LM, Leite, RB, Rodrigues, PM, Afonso, R, Cancela, ML. 2008. Effect of the
herbicide Roundup on Perkinsus olseni in vitro proliferation and in vivo survival when infecting a
permissive host, the clam Ruditapes decussatus. Bulletin of Environmental Contamination and
Toxicology 80: 512-515. IF=0.609.
165
3. Viegas, CS, Simes, DC, Laizé, V, Williamson, MK, Price, PA, Cancela, ML. 2008. Gla-rich protein
(GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly
conserved in vertebrates. Journal of Biological Chemistry 283: 36655-36664. IF=5.520.
4. Fonseca, PJ, Serrao, EA, Pina-Martins, F, Silva, P, Mira, S, Quartau, JA, Paulo, OS, Cancela, L.
mitochondrial DNA (Insecta, Hemiptera). Bioacoustics-the International Journal of Animal Sound
and Its Recording 18: 17-34. IF=0.722.
5. Coesel, SN, Baumgartner, AC, Teles, LM, Ramos, AA, Henriques, NM, Cancela, L, Varela, JCS.
2008. Nutrient limitation is the main regulatory factor for carotenoid accumulation and for Psy
and Pds steady state transcript levels in Dunaliella salina (Chlorophyta) exposed to high light and
salt stress. Marine Biotechnology 10: 602-611. IF=2.544.
Toxicology 24: 253-263. IF=2.155.
7. Tiago, DM, Cancela, ML, Aureliano, M, Laizé, V. 2008. Vanadate proliferative and anti
8. Tiago, DM, Laizé, V, Cancela, ML. 2008. Alternatively spliced transcripts of Sparus aurata
insulin-like growth factor 1 are differentially expressed in adult tissues and during early
development. General and Comparative Endocrinology 157: 107-115. IF=2.654.
9. Tanguy, A, Bierne, N, Saavedra, C, Pina, B, Bachère, E, Kube, M, Bazin, E, Bonhomme, F,
Boudry, P, Boulo, V, Boutet, I, Cancela, L, Dossat, C, Favrel, P, Huvet, A, Jarque, S, Jollivet, D,
Klages, S, Lapègue, S, Leite, R, Moal, J, Moraga, D, Reinhardt, R, Samain, J-F, Zouros, E, Canario,
A. 2008. Increasing genomic information in bivalves through new EST collections in four species:
408: 27-36. IF=2.578.
10. Leite, RB, Brito, AB, Cancela, ML. 2008. An oxygen molecular sensor, the HIF prolyl-4
hydroxylase, in the marine protist Perkinsus olseni. Protist 159: 355-368. IF=3.923.
11. Ishaq Ahmed, VP, Chandra, V, Parameswaran, V, Venkatesan, C, Shukla, R, Bhonde, R, Sahul
Hameed, AS. 2008. A new epithelial-like cell line from eye muscle of catla (Catla catla):
development and characterization. Journal of Fish Biology 72: 2026-2038. IF=1.246.
12. Rajesh Kumar, S, Ishaq Ahmed, VP, Parameswaran, V, Sudhakaran, R, Sarath Babu, V, Sahul
Hameed, AS. 2008. Potential use of chitosan nanoparticles for oral delivery of DNA vaccine in
Asian sea bass (Lates calcarifer) to protect from Vibrio (Listonella) anguillarum. Fish & Shellfish
Immunology 25: 47-56. IF=3.161.
13. Parameswaran, V, Rajesh Kumar, S, Ishaq Ahmed, VP, Sahul Hameed, AS. 2008. A fish
nodavirus associated with mass mortality in hatchery-reared Asian Sea bass, Lates calcarifer.
Aquaculture 275: 366-369. IF=1.678.
166
proceedings)
Books (Chapters)
1. Tiago, DM, Laizé, V, Aureliano, M, Cancela, ML. 2008. Vanadate effects on bone metabolism:
fish cell lines as an alternative to mammalian in vitro systems In: MA Alves (Ed). Vanadium
Biochemistry. Research Signpost, India, pp. 269-283.
2. Soudant, P, Leite, R, Chu, FLE, Villalba, A, Cancela, L. 2008. Bivalves-Perkinsus spp interactions.
In: A Villalba (Ed). Workshop for the analysis of the impact of Perkinsosis to the European
shellfish industry. pp. 78-109. Xunta de Galicia, Spain (commissioned and funded by the
European Community-FP6).
3. Garcia, C, Leite, R, Arzul, I. 2008. Host parasites of the genus Perkinsus. In: Workshop for the
analysis of the impact of Perkinsosis to the European shellfish Industry. Centro de Investigacións
Mariñas, Consellería de Pesca e Asuntos Marítimos da Xunta de Galicia, Vilanova de Arousa,
Spain. Centro Tecnológico del Mar - Fundación CETMAR, Vigo, Spain, pp. 77-110. Legal deposit:
VG- 274-2008.
Published proceedings
1. Conceição, N, Fazenda, C, Simões, B, Cancela, L. 2008. Transcriptional regulation of the
Xenopus laevis Matrix Gla protein gene. Calcified Tissue International 82: S139.
2. Viegas, CSB, Simes, DC, Williamson, MK, Laizé, V, Price, PA, Cancela, ML. 2008. Identification
of an MGP/OC hybrid gene in the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish
with a cartilaginous endoskeleton. Calcified Tissue International 82: S121-S122.
3. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2008. Fish as an alternative model to explore
bone biology: role of four and a half LIM domains 2 in tissue mineralization. Calcified Tissue
International 82: S115.
4. Simes, DC, Viegas, CSB, Williamson, MK, Price, PA, Cancela, ML. 2008. Purification of matrix
Gla protein and osteocalcin from the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish
with a cartilaginous endoskeleton. Bone 42: S17-S110.
proceedings)
MASTER THESES
Afonso, R. 2008. Estudo sobre aproveitamento biotecnológico de organismos marinhos. Master
thesis, University of Algarve. Co-supervisor: ML Cancela and R Leite.
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Madeira, C. 2008. Contribuição parental do linguado do senegal, Solea senegalensis (KAUP
1858), em aquacultura. Master thesis, University of Algarve. Co-supervisor: ML Cancela and S
Mira.
Rainha, R. 2008. Estudo da Variabilidade genética de marcadores moleculares ligados ao gene
da MGP e sua aplicação para paternidades em Sparus aurata. Master thesis in Marine Biology,
University of Algarve. Co-supervisor: ML Cancela and S Mira.
PhD THESIS
Ascenso, R. 2008. Study of Perkinsus olseni infection mechanisms: Identification and regulation
of parasite genes differentially expressed is response to host and environmental stress. PhD
thesis, University of Algarve. Co-supervisor: ML Cancela, G Vssta.
Tiago, DM. 2008. 2008. Role of insulin and insulin like peptides in bone formation: identification
of bone specific target genes and regulatory mechanisms, and characterization of the insulin
mimetic effect of vanadium. PhD thesis, University of Algarve. Co-supervisor: ML Cancela, V
Laizé and M Aureliano.
Molecular Biology of Marine Orgnisms Lab has been involved in the submission of two
international patents:
-
Inventors: Cancela, ML, Brito, AB, Gavaia, PJ, Afonso, N, Laizé, V. U.S. Patent: US
61/105.975. N.º confirmation: 8119. Date of deposit: 16 October 16 2008.
-
Inventors: Simes, DC, Viegas, CSB, Cancela, ML. U. S. Patent: US 61/136.315. Date of
deposit: 27 August 2008.
-
National Congress of Biochemistry. As president of the Portuguese Biochemical Society,
Leonor Cancela was involved in the organization of the national congress of the Society,
which was held in Ponta Delgada, Azores, 22-25 October 2008.
-
Seminários Mar e Ambiente 2008. Leonor Cancela was the coordinator of the PhD
seminars of the Faculdade de Ciências do Mar e Ambiente, Universidade do Algarve,
which was held in Faro, 8 May 2008.
-
XXXVII Annual Meeting of the Brazilian Biochemistry and Molecular Society and XI
Congress of PABMB. As president of the Portuguese Biochemical Society, Leonor
Cancela was involved in the organization of the symposium 22 “Skeletal
formation/mineralization during vertebrate development”, which was held in Águas de
Lindóia, SP, Brazil, 17-20 May 2008.
-
Interdisciplinary Approaches in Fish Skeletal Biology. To be held 27-29 April 2009 in
Tavira, Portugal ~100 participants from 12 countries.
168
April 2008, Research agreement with Novozymes, SA, Denmark. Identification of genes with
biotechnological applications.
Reseach related:
-
NoE Marine Genomics Europe: Fish & Shellfish node partner, coordination of flagship
project FICEL (270 k€, 4 institutes). Outcomes include project FP7 I -infrastructure
“ASSEM LE” (10 M€, 8 institutes, approved). Major role in developing cDNA libraries
from seabream and clam sequenced at the Max Plank Institute.
-
COST ACTION B23 (L Cancela national representative) and COST ACTION FA0801
Larvanet.
-
Project evaluations (e.g. EC, Treaty of Windsor, INRA, CRSNG, French National Research
Agency, Dutch Research Council-NWO, INTAS), journal editor (J 15 journals, Bone
Mineral Research, IF 6.004) and referees.
-
Collaborative actions with groups in Spain, UK, France, USA (FCT, CRUP, GRICES, British
Council, FLAD).
-
Oral communications + plenary lectures at international conferences (~19).
Training:
-
Training of 7 MSc & 9 PhD students, 5 post-docs and nternational students.
-
Teaching of undergraduate ERASMUS/SOCRATES students in ongoing University
degrees.
-
Hosting visiting researchers: 2 sabbaticals, ~25 short term training (Norway, Germany,
Spain, France, USA).
-
Coordination of a course on Biology of Bone and Cartilage (Master degree in Biomedical
sciences, UALG). Teaching involved lecturers from France, Germany and
elgiumParticipation as “Raporteur” of one PhD thesis in Medicine, University Paris 7.
France (12-2008).
Research dissemination:
-
13 international publications, 19 communications in international meetings,
organization of 2 international symposiums.
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Invited Lectures:
-
Cancela ML. Workshop Fish in Experimental Research, March 2008, IMM, Faculty of
Medicine, Lisbon.
-
Cancela ML. Collaborative seminars series, March 2008, Faro Central Hospital and UALG,
Faro.
-
Cancela ML. Invited Seminar, October 2008, IMM, Faculty of Medicine, Lisbon.
-
Cancela ML. Invited Seminar, December 2008, Lariboisière Hospital, Paris, France.
Public Awareness:
-
Research featured in Hatchery International 09/10 2008 issue pp11-12.
-
LAB-IT®-The Itinerant Laboratory in Molecular Genetics. Project aiming to bring
molecular biology and genetics to high schools. A collaboration between UALG, EDGE
laboratory, Direction of the Education Board in Algarve, the centers for Training High
school teachers and various high schools in the Algarve region.
Cancela ML (January 2005 - December 2008): President of the Portuguese Biochemistry Society,
a non-profit, nationally recognized organization promoting the development and dissemination
of biochemistry in Portugal. Of particular relevance has been the organization of National
Biochemistry meetings and special interest meetings (i.e. Clinical Biochemistry, Biochemistry of
RNA, training courses), joint meetings with other Biochemistry societies (Spanish, English),
promoting education in Biochemistry.
Cancela ML: National representative of the Portuguese Society of Biochemistry in the
International Union of Biochemistry and Molecular Biology (IUBMB) and the Pan American
Biochemistry and Molecular Biology Association (PABMB).
Cancela ML: National representative of the FP6-funded COST action B 23 (New Frontiers in Oral
Facial Regeneration).
8e. Future research
1. Objectives
I. Molecular determinants of mineralization/calcium mobilization (ML Cancela, V Laizé, DM
Tiago, P Gavaia, D Simes, N Conceição, PM Rodrigues)
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Genes & regulatory pathways involved in skeletogenesis, environmental adaptations, tissue
regeneration (ML Cancela, V Laizé, DM Tiago, P Gavaia, N Conceição)
-
Using zebrafish & seabream as model systems, integrated approach, functional
genomics, proteomics, bioinformatics, in-house developed tools including new cell lines,
DNA microarrays, microRNA arrays, antibodies, cDNA and genomic libraries.
Collaboration with 18 groups from 9 countries.
Family of Gla-containing proteins: new members, evolution, function in health & disease (ML
Cancela, D Simes)
-
Development of immuno-based diagnostic assays for new antigen detection in biological
samples relevant for human health (Viegas et al, 2008; patent pending). Collaboration
with USA & Portuguese hospitals.
New osteocalcin isoforms (V Laizé, D Simes)
-
Being first to identify existence of osteocalcin isoforms (Laizé et al, 2006), our goal is to
complete this study by identifying their function & evolution. Collaboration with USA.
Gene expression & skeletal deformities (P Gavaia, D Simes, PM Rodrigues)
-
Development of diagnostic tools suitable to detect skeletal malformations in fish
through genomic, proteomic, microarray, histological-based tools (Roberto et al,
submitted). Collaboration with 7 groups (including CCMAR-Aquaculture group) from 6
countries.
Proteins from oyster nacre with osteoinductive function (D Simes)
-
Following our identification of nacre proteins as in vitro mineralization promoters their
molecular structure, regulation & function will be analysed.
New cell lines from model fish species (V Laizé, ML Cancela)
-
Following development of FIRST fish bone-derived cell lines (seabream; Pombinho et al.
2004), we now seek at developing additional lines from other fish (e.g. zebrafish, sole,
salmon) requiring in vitro homologous systems (FP7 project ASSEMBLE, funded).
II. Unveiling Perkinsus spp (PE) metabolic pathways suitable as therapeutic targets to limit
infection and for biotechnological applications (ML Cancela, R Leite)
-
Study of PE intracellular machinery, PE as model for toxicological/metabolic/genomic
studies, expression/silencing of PE genes involved in virulence, PE potential as
unicellular factory to synthesize bio-molecules of economical interest. Collaboration
with 7 groups from 5 countries.
III. Accessong wild population’s variability, sex, parental gamete contribution, genetic diversity
(S Mira, ML Cancela)
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-
Goals based on previous achievements including: i) Identification of multiple paternity in
Norway lobster and correlation with degree of stock exploitation, ii) Differential
contributions of fish brood stock for progeny and implementation of selective breeding
programs, and iii) Survey/sexing/assessment of onelli’s eagle and otter populations for
management of wild populations. Collaboration in LIFE & FCT projects with 6 groups
from 4 countries.
IV. Organization of the first international workshop on fish skeletogenesis (EDGE LAB)
-
To be held April 27-29, 2009 in Tavira, Portugal (by invitation only) ~100 participants
from 12 countries.
2.

Current funding
83.450 EUR, PTDC/MAR/70855/2006-FATTYBONE, 08/2008-07/2011.
60.000 EUR, PTDC/MAR/71685/2006-HYDRAA, 04/2008-03/2011.
EU-FP7/227799-ASSEMBLE, 13.971.049 EUR/CCMAR 2.668.249 EUR, 07/2008-06/2011.
60.000 EUR, PTDC/MAR/70858/2006-TEXBREAM, 06/2008-05/2011.
10.000 EUR, PTDC/MAR/64533/2006-CRYOSPERM, 08/2008-07/2011.
161.115 EUR, PTDC/CVT/72083/2006-ISOPERK, 08/2007-07/2010.
COST ACTION FA0801-LARVANET, budget: n/a.
MGE Consumable grant, 5750 EUR, 5000 EUR, 5000 EUR.

Pending funding
135.875 EUR, PTDC/MAR/102269/2008-DEEPBONE.
177.267 EUR, PTDC/MAR/105313/2008-FISHCELL.
165.604 EUR, PTDC/BIA-BCM/104653/2008-BONEmiR.
183.893 EUR, PTDC/BIA-BCM/104708/2008-GRPCELL.
165.707 EUR, PTDC/BIA-BCM/103725/2008-MEFTARGET.
150.371 EUR, PTDC/SAU-OSM/101195/2008-SERUMSN.
195.107 EUR, PTDC/MAR/105152/2008-SPECIAL-K.
172
199.992 EUR, PTDC/CVT/109174/2008-REFERENCE-Z.
198.693 EUR, PTDC/BIA-BCM/101662/2008-XENOFISH II.
189.694 EUR, PTDC/MAR/099126/2008-BIOFISH.
199.664 EUR, PTDC/SAU-NEU/099133/2008-BIOHUNT.
188.114 EUR, PTDC/CVT/104320/2008-Transkriperk.
182.319 EUR, PTDC/SAU-OBD/105710/2008-ZeFusion.
157.200 EUR, PTDC/SAU-OSM/101195/2008-OSTEOARTGRP.

Participation of EDGE
100.000 EUR, PTDC/SAU-FCF/103033/2008-PROTEOGLU.
80.583 EUR, PTDC/CVT/102481/2008-ULTRAFISH.
26.750 EUR, PTDC/MAR/104042/2008-ECOLARVAE.
PTDC/MAR/098496/2008-EPISOLE, CCMAR 107.829 EUR.
PTDC/ECM/103882/2008, EDGE 39.000 EUR.
36.000 EUR, PTDC/QUI-BIQ/102065/2008.
45.000 EUR, PTDC/CVT/103644/2008-WELLIND.
67.066 EUR, ReDUSK - PTDC/MAR/098659/2008.
50.000 EUR, ECOAQUA, INTERREG.
FP7-SME-2008-1-PROSPAWN, EU, CCMAR 99.000 EUR.
173
Fish Nutrition
Fish Nutrition
Aires Manuel Pereira Oliva Teles
-
-
National funding
6.536,74 EUR, FCT POCI/CVT/57695/2004, Utilisation of dietary carbohydrates by sea bass
(Dicentrarchus labrax) and sea bream (Sparus aurata) juveniles. PI: A Oliva Teles (FCUP; CIIMAR)
and H Peres (CIIMAR). Participating Institutions: FCUP, CIIMAR; participants of the Laboratory of
Nutrition: A Oliva Teles, H Peres, P Enes.
disrupting chemicals in two fish species with different life histories. PI: M Santos (Environmental
Toxicology Lab.). Participating Institutions: CIIMAR; participants of the Laboratory of Nutrition:
AP Carvalho.
Agência de Inovação, OPTISOLE - Investigação e desenvolvimento na produção de juvenis de
linguado: incremento da resistência a patologias e do crescimento. PI: A Afonso (CIIMAR).
Participating Institutions: CIIMAR; FCUP; ICBAS; participants of the Laboratory of Nutrition: A
Oliva Teles, H Peres, AP Carvalho.
1. Objectives
The fish nutrition group directs its research to the study of basic and applied aspects of the
nutrition and feeding of larvae and juveniles of fish species well established in marine
174
aquaculture (sea bass, sea bream, turbot), candidate species for aquaculture (sargus sp., sole),
and ornamental species (zebrafish, angelfish, kribensis, jewelfish).
Main research activities are directed towards the study of:
-
Protein and amino acid requirements, metabolism and utilization efficiency;
-
Utilization of conventional energy sources (lipids and carbohydrates);
-
Phosphorus requirement and bioavailability;
-
Potential use of feedstuffs alternative to fish meal
-
Nutritional strategies for health and welfare improvement;
-
Development of microparticulate diets for fish larvae and weaning strategies.
These studies aim contributing for a deep insight on the nutritional requirements and metabolic
utilization of nutrients by fish, therefore providing basic information regarding the physiological
aspects of comparative animal nutrition and contributing for a scientific based improvement of
fish diets. From an applied perspective, studies aim maximization of fish growth and feed
utilization while reducing the environmental impact due to feeding. This way it is expected to
contribute for the sustainable development of aquaculture.
During this year studies have advanced concerning a deeper understanding of the effect of
temperature on the metabolic utilization of carbohydrate in gilthead sea bream and European
sea bass (Publication nr 1, 4, 5, 7 – Publications in peer review journals) and the effect of
complexity of starch (normal or waxy) on carbohydrate utilization by gilthead sea bream and
white sea bream (Publication nr 3, 11 – Publications in peer review journals).
Regarding alternative protein sources utilization, the effect of thermal treatment on the
utilization of pea seed meal and the effect of rapeseed meal and cotton seed meal inclusion in
diets for African catfish were evaluated (Publication nr 2 – Publications in peer review journals;
Publications nr 3 - Other publications international) as well as potential of combining blood meal
with other animal feedstuffs as alternative for fish meal (Publications nr 3 - Other publications
international).
As for nutrient requirements, dietary protein requirement for white sea bream and two banded
sea bream were studied (Publication nr 9 – Publications in peer review; Ozório et al. 2009.
Aquaculture Nutrition 15: 85-93), adequate dietary lipid level for white sea bream (Publication
nr 10) and lysine requirement of turbot (Publication nr 8) were studied.
Concerning health effects on fish, the toxic effects of cyanobacteria on early developmental
stages of carp (Osswald, J et al. 2009. Ecotoxicology and Environmental Safety 72: 473-478) and
on compensatory growth of zebrafish (El Ghazali, I et al. 2009. International Journal of Molecular
Sciences 10(1): 133-146) were also analyzed.
175
Reviews were also published making an up to date analysis of the nutritional regulation of
hepatic glucose metabolism in fish (Enes, P et al. 2009. Fish Physiology and Biochemistry, doi
10.1007/s10695-008-9259-5) and the use of soybean products in fish diets (Publications nr 1, 2 Other publications international).
8d. Productivity
1. Couto, A, Enes, P, Peres, H, Oliva-Teles, A. 2008. Effect of water temperature and dietary
carbohydrate level on growth performance and metabolic utilization of diets in gilthead sea
bream (Sparus aurata) juveniles. Comparative Biochemistry and Physiology - Part A 15: 45-50.
IF=1.709.
2. Davies, SJ, Gouveia, A. 2008. Enhancing the nutritional value of pea seed meals (Pisum
sativum) by thermal treatment or isogenic selection in comparison with soybean meal for
African catfish, Clarias gariepinus. Aquaculture 283: 116-122. IF=1.678.
3. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Growth performance and metabolic
utilization of diets with native and waxy maize starch by gilthead sea bream (Sparus aurata)
juveniles. Aquaculture 274:101-108. IF=1.678.
4. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Hepatic glucokinase and glucose-6phosphatase responses to dietary carbohydrate sources in gilthead sea bream (Sparus aurata)
juveniles at different rearing temperatures. Comparative Biochemistry and Physiology - Part A
149: 80-86. IF=1.709.
5. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Rearing temperature enhances hepatic
glucokinase but not glucose-6-phosphatase activities in European sea bass (Dicentrarchus
labrax) and gilthead sea bream (Sparus aurata) juveniles fed with the same levels of glucose.
Comparative Biochemistry and Physiology - Part A 150: 355-358. IF=1.709.
6. Morais, J, Augusto, M, Carvalho, AP, Vale, M, Vasconcelos, VM. 2008. Cyanobacteria
environmental conditions. European Food Research and Technology 227: 949-952. IF=1.622.
7. Moreira, IS, Peres, H, Couto, A, Eenes, P, Oliva-Teles, A. 2008. Temperature and dietary
carbohydrate level effects on performance and metabolic utilisation of diets in European sea
bass (Dicentrarchus labrax) juveniles. Aquaculture 274: 153-160. IF=1.678.
8. Peres, H, Oliva-Teles, A. 2008. Lysine Requirement and Efficiency of Lysine Utilization in
Turbot (Scophthalmus maximus) Juveniles. Aquaculture 275: 283-290. IF=1.678.
9. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A, 2008. Dietary protein requirement of white sea
bream (Diplodus sargus) juveniles. Aquaculture Nutrition 14: 309-317. IF=1.398.
176
10. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A. 2008. Dietary lipid utilisation by white sea bream
(Diplodus sargus) juveniles. Journal of the World Aquaculture Society 39: 423-428. IF=0.693.
11. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A. 2008. Effect of dietary starch source (normal vs.
waxy) and protein levels on performance of white sea bream (Diplodus sargus) juveniles.
Aquaculture Research 39: 1069-1076. IF=0.991.
proceedings)
1. Peres, H, Lim, C. 2008. Use of soybean products in non-salmonids marine finfish. In: Lim, CE,
Webster, CD, Lee, CS (Eds). Alternative Protein Sources in Aquaculture Diets. Haworth Press,
New York, pp. 281-312.
2. Brown, PB, Kaushik, S, Peres, H. 2008. Protein feedstuffs originating from soybeans. In: Diets.
Lim, CE, Webster, CD, Lee, CS (Eds). Alternative Protein Sources in Aquaculture. Haworth Press,
New York, pp. 205-224.
3. Davies, SJ, Gouveia, A. 2008. Depression of dietary nitrogen utilization in African catfish,
Clarias gariepinus resulting from rapeseed and cottonseed meal inclusion in experimental diets.
Journal of Fisheries International 3(4): 98-104.
4. Laporte, J, Woodgate, S, Davies, S, Serwatar, R, Gouveia, A. 2008. Combining Blood Meal with
other animal protein sources in compound aqua-feeds: an alternative solution for the reduction
of fishmeal in nutritionally balanced diets. International Aquafeed 11(4). 12-18.
proceedings)
MASTER THESES
Borges, C. 2008. Estudos de adaptação ao alimento artificial em larvas de peixes ciclídeos
ornamentais: escalar (Pterophyllum scalare) e kribensis (Pelvicachromis pulcher). Master thesis
in Biology and Management of Marine Resources (Fisheries and Aquaculture), Faculty of
Sciences, University of Lisbon. Supervisor: AP Carvalho.
PhD THESIS
Enes, PC. 2008. Utilization of dietary carbohydrates by European sea bass (Dicentrarchus labrax
L.) and gilthead sea bream (Sparus aurata L.) juveniles. PhD thesis in Biology, Faculty of Sciences,
University of Porto. Supervisor: A Oliva-Teles.
177
Research cooperation is well established with:
-
Prof. SJ Davies, Faculty of Sciences, Plymouth University.
-
Dr. SJ Kaushik and Dr. S. Panserat, INRA, St. Pée sur Nivelle, France.
-
Prof. AE Morales, Faculty of Sciences, Granada University.
-
Prof. Vera Cruz Rubio, University of Murcia.
Recently, the following contacts have been established aiming the prosecution of works in new
directions and integrating Pos-Doc and PhD students:
-
Prof. J Gutierrez, University of Barcelona, as co-supervisor with A. Oliva- Teles of the
post-doc of Paula Enes;
-
Prof. A Krogdahl, Norwegian Science and Veterinary Science, Aquaculture Protein
Center, as co-supervisor with A Oliva-Teles of Ana Couto PhD. thesis.
8e. Future research
1. Objectives
Research within the next few years will continue to fall across the main research areas that were
defined in our group as research priorities.
Research focused in commercial aquaculture species such as sea bass and sea bream juveniles
will be focused on:
178
-
Deeper analysis on amino acid requirements and amino acids utilization;
-
Further understanding of carbohydrate metabolism regulation;
-
Evaluation of the antioxidative stress due to feedstuffs and additives and its effect on
the innate defence mechanisms;
-
Evaluation of feedstuffs utilization and of the effect of anti-nutrients on gut morphology
and gut metabolism;
-
Use of probiotics and its effect on the immune system;
-
Further understanding of phosphorus utilization and uptake regulation.
In new species for aquaculture we will study:
-
The effect of temperature on growth and nutrient utilization of Senegalensis sole;
-
The utilization of carbohydrate rich plant feedstuffs in omnivorous species such as
Diplodus sargus;
-
Establish the macronutrient requirements and utilization of new species for
aquaculture, such as Diplodus cervinus and Solea senegalensis.
Such studies will be carried out using conventional approaches already in use in our lab but we
also aim to apply molecular approaches, namely nutrigenomics and proteomics that may give a
new insight in the elucidation of such aspects. Aspects related to nutritional impact on fish
health will also be prosecuted. All this implies a multidisciplinary approach, therefore a close
collaboration with colleagues both from CIIMAR and external laboratories (national and
international) will be necessary.
Nutrigenomics is a promising field in fish nutritional studies and a qualitative up-grade in CIMAR
skills would greatly benefice if an expert in this field is contracted.
2.

Current funding
Agência de Inovação, OPTISOLE - Investigação e desenvolvimento na produção de juvenis de
linguado: incremento da resistência a patologias e do crescimento. PI: A Afonso (CIIMAR).
Participating Institutions: CIIMAR; FCUP; ICBAS; participants of the Laboratory of Nutrition: A
Oliva Teles, H Peres, AP Carvalho.
179

Pending funding
PTDC/MAR/099534/2008, Utilization of plant products in diets for European seabass
(Dicentrarchus labrax) and white seabream (Dioplodus sargus) juveniles: potential and
challenges. PI: H Peres.
180
Fisheries Biology and Hydroecology Research Group (FBHRG)
Fisheries Biology and Hydroecology Research Group (FBHRG)
José Pedro de Andrade e Silva Andrade
6.000 EUR, FCT Pluriannual funding (to JP Andrade, J Palma and A Sykes).
1. Objectives
-
To enhance the number and quality of cuttlefish eggs obtained in captivity, using
increased bottom areas.
-
To increase survival rates at hatching and during early development of cuttlefish, using
different light intensities.
-
To continue previous research on the use of Caridae shrimps, specifically the Atlantic
Ditch Shrimp (Palaemonetes varians), as a new live feed to be used in aquaculture and
also for human consumption, with emphasis on larval rearing and the production of
artificial diets.
-
To increase the technological and scientific basis for artificial reproduction, larval
maintenance and broodstock management in longsnout seahorse, Hippocampus
guttulatus, and Syngnathus spp.
-
To study the reproductive behavior of H. guttulatus in the Ria Formosa.
-
To use microsatellites as genetic markers to study egg collapse in consecutive
generations of cuttlefish.
-
To increase efforts to get funding for group research.
181
-
To engage pos-graduation students in the research activities of the FBHRG.
Regarding cuttlefish rearing, the total amount of eggs was increased as well as its quality by
using tanks with extended bottom areas. Although we are still analyzing the results, these
clearly show an increment of egg number and viability. For instance, one of the bigger tanks
displayed the highest amount of eggs ever obtained in our facilities (14312 eggs) and the lowest
non-viability (0.19% in terms of the first classification based on egg color and shape). As for the
hatchling experiment and cuttlefish, survival increased to values near 100% and biomass
production was maximized in tanks with lower light intensities (100 lux).
Within the shrimp research, results indicated that high survival rates can be attained within a
temperature range close to 20ºC, even with rearing densities of 50 larvae l-1. The survival and
growth of early weaned larvae fed with a practical artificial diet was also verified and it is a
promising step ahead in the development of the culture of this species. The dietary
requirements for maximizing weight gain of juvenile P. varians, and expressed as percent of dry
diet, were: 2.4, 0.9 and 1.9 for lysine, methionine and arginine, respectively.
Regarding the seahorse research, the primary objectives were achieved, and results on the
improvement of initial survival of H. guttulatus fry, the effect of broodstock nutrition on the
seahorse reproduction and brood quality and the use of enriched shrimp as a diet of juvenile
and adult seahorses was obtained.
A comparison of the effect of culture tanks background on feeding efficiency at 1 week and 1
month of age using Artemia as prey, showed an age-dependent effect on both the attack rate
and the capture success. Results also showed that male seahorses fed with a combined natural
and artificial diet generated more 87) and significantly bigger friesbroods (9) more fries per
brood (299 0.1 mm) than males fed only natural diets. Regarding the use of enriched18.1
shrimp diets on the fitness condition of H. guttulatus, the combined use of a natural diet
(shrimp) filled with an artificial diet, not only provides higher growth rates, but it is also a
practical co-feeding of two diets in just one item, and can be regarded as a step ahead in
seahorse nutrition.
Microsatellites previously cloned for S. officinalis were isolated in a total of 64 samples of
cultured cuttlefish, from wild to F2 generations. Protocols were adjusted to the samples and are
now ready for systematic use in the remaining samples to be processed
8d. Productivity
1. Correia, M, Palma, J, Andrade, JP. 2008. Effects of live prey availability on growth and survival
in the early stages of cuttlefish Sepia officinalis (Linnaeus, 1758) life cycle. Aquaculture Research
39(1): 33-40. IF=0.991.
182
2. Nunes, MA, Santos, S, Cordeiro, JM, Neves, P, Silva, VS, Sykes, AV, Morgado, F, Dunant, Y,
Gonçalves, PP. 2008. Acetylcholine release and choline uptake by cuttlefish (Sepia officinalis)
optic lobe synaptosomes. Biological Bulletin 214: 1-5. IF=1.949.
3. Correia, M, Palma, J, Kirakowski, T, Andrade, JP. 2008. Effects of prey nutritional quality on
growth and survival of juvenile cuttlefish, Sepia officinalis (Linnaeus, 1758). Aquaculture
Reasearch. Aquaculture Research 39: 869-876. IF=0.991.
4. Palma, J, Bureau, D, Andrade, JP. 2008. Effects of binder type and binder addition on the
growth of juvenile Palaemonetes varians and Palaemon elegans (Crustacea: Palaemonidae).
Aquaculture International 16: 427-436. IF=0.608.
5. Palma, J, Correia, M, Andrade, JP. 2008. Usefulness of flat bottom tanks on the settlement of
spider crab (Maja squinado, Herbst) larvae. Aquaculture Research 39: 1005-1008. IF=0.991.
6. Palma, J, Stockdale, J, Correia, M, Andrade, J. 2008. Growth and survival of adult long snout
seahorse (Hippocampus guttulatus) using frozen diets. Aquaculture 278: 55-59. IF=1.678.
7. Esteves, E, Andrade, JP. 2008. Diel and seasonal distribution patterns of eggs, embryos and
larvae of Twaite shad Alosa fallax fallax (Lacépède, 1803) in a lowland tidal river. Acta
Oecologica 34: 172-185. IF=1.456.
proceedings)
proceedings)
183
FBHRG participated in a meeting in Tenerife (October) with other worldwide cephalopod
aquaculture researchers for the elaboration of an EU project proposal (KBBE call for 2011) and
network (COST action).
The FBHRG collaborated with the Department of Animal and Poultry Science of the University of
Guelph, Canada, on the research for new artificial diets for seahorses.
The FBHRG collaborated with researchers of Evonik/Degussa – Chemicals on the applied
research on amino acid determination and requirements for Caridae Shrimp.
Collaborative research with Project Seahorse, UBC, Canada and the Zoological Society of
London, aiming to study seahorse movement patterns and their connection to environmental
conditions in order to help future researchers and con¬servationists predict locations of
European seahorse popula¬tions. A memorandum of understanding will be established, during
2009, between the the coordinators of the Project Seahorse, Prof. Amanda Vincent, and the P.I
of the FBHRG, JP Andrade.
Collaboration has been established among the FBHRG, the Universidad Pablo de Olavide (Seville,
Spain) and the Estación Biológica de Doñana (Seville, Spain) to develop joint research on the use
of microsatellites in inbreeding and parentage analysis studies.
8e. Future research
1. Objectives
To determine if cuttlefish may be fed with frozen grass shrimp from the first DAH.
To further understand the metabolism at different life stages. To continue to study the
reproduction of the species under captivity in order to establish a breeder protocol.
To use QIM schemes already developed for cuttlefish, together with microbiological and
chemical/nutritional analyses, to study the deterioration of specimens cultured in different
conditions of temperature, feed and/or ration.
To compare the above information with wild-caught, fishery-originated individuals.
To determine the seahorse digestibility, when fed with artificial diets.
To detail and update the rearing conditions to improve the seahorse fry survival. To describe the
breeding behaviour, breeding cycle and sexual behaviour of the H. guttulatus.
184
To a assess the viability of re-stocking as a management option for the recovery of seahorse
populations (H. guttulatus) in the Ria Formosa as a model species. The following subsidiary
objectives will be considered:
-
The status of seahorse populations in the Ria Formosa;
-
Variation of seahorse populations over different temporal and spatial scale;
-
Threats to seahorse populations in the Ria Formosa;
-
Culture/ranching techniques;
-
Effectiveness of population supplementation.
To use microsatellites to investigate the effects of inbreeding in consecutive generations of
cultured cuttlefish.
To use microsatellites to study sexual selection mechanisms in both wild and captive seahorses.
2.

Current funding
4.032 EUR, Oceanário de Lisboa, Project title: Conserving seahorses in the wild and captivity: the
influence of environment and movement.
185
Karim Erzini
124.916 EUR, ARH Algarve, RENSUB IV, Cartography and characterization of the marine
communities of the National Underwater Ecological Reserve between the Arade River and Ponta
da Piedade, 2009-2011.
254.388 EUR, EU /Secil, LIFE Project - PTCON0010, Restoration and Management of Biodiversity
in the Marine Park Site Arrábida-Espiche, 2007-2010.
Canada Foundation for Innovation $ 35 million, Ocean Tracking Network - OTN. Project will
provide telemetry equipment (unspecified amount - still being negotiated), 2007-2011.
103.279 EUR, CCDR Algarve, RENSUB III, 2007-2008.
50.000 EUR, FCT POCTI/CTA/549248/2002 - Environmental impact of static fishing gears of the
south-west coast of Portugal: reconciling fishing with conservation of marine ecosystems, 20062008.
53.333 EUR, INTERREG IIIA, PROMOPESCA, 2006-2008.
76.500 EUR, FCT POCI/MAR/58157/2004, SPORTFIH, 2005-2008.
86.500 EUR, FCT POCI/BIA-BDE/61949/2004, TELEMETRIA, 2005-2008.
9.300 EUR, FCT POCI/BIA-BDE/59426/2004, LobAssess, 2005-2009.
60.720 EUR, FCT PDCT/MAR/59366/2004, SURVIVAL, 2007-2009.
186
1. Objectives
Fishing and fishing related activities are of considerable importance in the Algarve (southern
Portugal), which is the Portuguese region with the highest percentage of the working population
directly or indirectly involved in fisheries. The main objectives of the group are to contribute to
the improved management and sustainable exploitation of the multi-species resources of the
Algarve coastal waters. In recent years, the fisheries sector has been under considerable
pressure, with a decline in landings of many of the most important species. Within this context,
the main objectives of the group are to contribute to the improved management and
sustainable exploitation of the multi-species resources of the Algarve coastal waters. Specific
objectives include:
-
Studying the impact of commercial fishing activity and the implications for biodiversity:
species and size selectivity of different gears, quantification of the by-catch and discards
of different gears, and evaluation of ghost fishing;
-
Studying the impact of recreational fishing activity;
-
Finding ways to mitigate by-catch, discarding and to propose management measures
and changes in legislation based on the above mentioned studies;
-
Biology, population dynamics, and ecology of fish and invertebrates: growth and
mortality parameters, maturity and reproduction, feeding ecology;
-
Identification of essential fish habitat, by classification of the bottom, mapping of the
biodiversity and distribution and abundance of key species;
-
Long-term monitoring of key habitats and recruitment of fish and invertebrates;
-
Evaluation of the effects of marine protected areas;
-
Understanding the spatio-temporal dynamics of key commercial species by means of
tagging and telemetry studies;
-
Modeling and simulation studies for spatio-temporal dynamics, eco-trophic modeling,
evaluating the effects of different management measures, and understanding the
influence of environmental and fisheries factors.
In 2008, work continued on the mapping of the Algarve coast to a depth of 30m, with
classification of the bottom and identification of fish, invertebrates and algae (1137 species,
including new species and new records for Portugal and Europe), and data implemented in a
Geographic Information System (GIS).
The group has now completed a series of projects spanning a period of more than 10 years on
the impacts in terms of by-catches and discarding, species and size selectivity of all the main
187
gears used in the Algarve. For some gears (deepwater hake longline, demersal purse seine and
bottom trawling), gear modifications for the reduction by-catch have been successfully tested,
with the collaboration of commercial fishermen and other research institutions such as the
IPIMAR.
The biology, population dynamics and ecology of a number of species of fish, cephalopods and
crustaceans have been studied, namely commercially important sea breams (Sparidae),
deepwater sharks, octopus and Norway lobster. Estimated parameters have been used in stock
assessments for some species and for simulation based studies to evaluate different
management measures.
A project on recreational / sport fishing was completed in 2008, resulting in the first estimates
of the impacts (total catches, species composition, catch rates, sizes of fish) of this activity in the
south of Portugal.
Acoustic telemetry has been used to study the spatio-temporal dynamics of sea bream species
within the Ria Formosa, and to evaluate the artificial reef use by wild and cultured (restocked)
sea breams.
Annual monitoring of key nursery habitats (Ria Formosa lagoon (since 2001) and Arade river
estuary (since 2003) has been carried out. These time series will provide important information
on recruitment variability and will be useful for evaluating climate change effects.
Monitoring of fish and invertebrates in different protection areas of the Arrábida Marine
Protected Area continued in 2008, along with onboard monitoring of commercial fishing activity.
Alternatives to traditional management based on biological indicators, and management
actively involving fisherman are being tested through collaborations with other research groups,
and are part of a series of Master’s and PhD thesis.
A number of projects were submitted and new projects were initiated (see below).
8d. Productivity
17 publications in 2008:
1. Abecasis, D, Bentes, L, Coelho, R, Correia, C, Lino, PG, Monteiro, P, Gonçalves, JMS, Ribeiro, R,
Erzini, K. 2008. Ageing seabreams: comparative study between scales and otoliths. Fisheries
Research 89: 37-48. IF=1.434.
2. Abecasis, D, Erzini, K. 2008. Site fidelity and movements of gilthead sea bream (Sparus aurata)
in a coastal lagoon (Ria Formosa, Portugal). Estuarine, Coastal and Shelf Science 79: 758-763.
IF=2.072.
188
3. Almeida, C, Coelho, R, Silva, M, Bentes, L, Monteiro, P, Ribeiro, J, Erzini, K, Gonçalves, JMS.
2008. Use of different intertidal habitats by faunal communities in a temperate coastal lagoon.
4. Coelho, R, Erzini, K. 2008. Effects of fishing methods on deep water shark species caught as
by-catch off southern Portugal. Hydrobiologia 606(1): 187-193. IF=1.449.
5. Coelho, R, Erzini, K. 2008. Identification of deep water lantern sharks (Chondrichthyes:
Etmopteridae) using morphometric data and multivariate analysis. Journal of the Marine
Biological Association of the United Kingdom 88: 199-204. IF=1.056.
6. Coelho, R, Erzini, K. 2008. Life history of a wide ranging deep water lanternshark in the NE
Atlantic, Etmopterus spinax (Chondrichthyes: Etmopteridae), with implications for conservation.
Journal of Fish Biology 73: 1419–1443. IF=1.246.
7. Costa, ME, Erzini, K, Borges, TC. 2008. Bycatch of crustacean and fish bottom trawl fisheries
from southern Portugal (Algarve). Scientia Marina 72: 802-814. IF=1.075.
8. Erzini, K, Bentes, L, Coelho, R, Lino, PG, Monteiro, P, Ribeiro, J, Gonçalves, JMS. 2008. Catches
in "ghost-fishing" octopus and fish traps in the north-eastern Atlantic (Algarve, Portugal). Fishery
Bulletin (U.S.) 106: 321-327. IF=1.190.
9. Fraussen, K, Afonso, CML. 2008. A new Euthria (Gastropoda:Buccinidae) from the Cape Verde
Archipelago. Gloria Maris 47: 53-60.
10. Gonçalves, JMS, Bentes, L, Monteiro, P, Coelho, R, Corado, M, Erzini, K. 2008. Reducing
discards in a demersal purse-seine fishery. Aquatic Living Resources 21: 135-144. IF=0.667.
11. Gonçalves, JMS, Bentes, L, Coelho, R, Monteiro, P, Ribeiro, J, Correia, C, Lino, PG, Erzini, K.
2008. Non-commercial invertebrate discards in an experimental trammel net fishery. Fisheries
Management & Ecology 15: 199-210. IF=1.404.
12. Hazin, H, Erzini, K. 2008. Assessing swordfish distribution in the South Atlantic from spatial
predictions. Fisheries Research 90: 45-55. IF=1.434.
13. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2008. The effect of predation on artificial reef
juvenile demersal fish species. Marine Biology 153: 1233-1244. IF=1.953.
14. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2008. Fish assemblages and rapid colonisation
after enlargement of an artificial reef off the Algarve (Southern Portugal) coast. Marine Ecology
28: 435-448. IF=1.953.
15. Monteiro, P, Ribeiro, D, Silva, JA, Bispo, J, Gonçalves, JMS. 2008. Ichthyofauna assemblages
from two unexplored Atlantic sea mounts: Northwest Bank and João Valente Bank (Cape Verde
Archipelago). Scientia Marina 72: 133-143. IF=1.075.
16. Oliveira, F, Almeida, C, Gonçalves, JMS. 2008. Ascidonia flavomaculata (Heller, 1864), a new
record for the Portuguese marine invertebrate fauna (Decapoda, Pontoniidae). Crustaceana 82:
63-67. IF=0.450.
189
17. Ribeiro, J, Monteiro, CC, Monteiro, P, Bentes, L, Coelho, R, Gonçalves, JMS, Lino, PG, Erzini,
K. 2008. Long-term changes in fish communities of the Ria Formosa coastal lagoon (southern
Portugal) based on two studies made 20 years apart. Estuarine Coastal and Shelf Science 76: 5768. IF=2.072.
proceedings)
1. Abecasis, D, Afonso, C, Almeida, C, Bentes, L, Monteiro, P, Oliveira, F, Veiga, P, Erzini, K,
Gonçalves, JMS. 2008. Cartography and characterization of the marine communities off the
National Underwater Ecological Reserve from the Algarve (South Portugal). Proceedings of the
2nd International Symposium on Underwater Research, Famagusta, Cyprus.
2. Coelho, R. 2008. [PhD. Summary]: Biology, population dynamics, management and
conservation of deep water lantern sharks, Etmopterus spinax and Etmopterus pusillus
(Chondrichthyes: Etmopteridae) in southern Portugal (northeast Atlantic). Cybium 32: 282.
3. Hazin, H, Erzini, K. 2008. Análisis de series temporales multivariantes cortas: aplicación de
MAFA (Análisis de autocorrelación min/max factorial) y DFA (análisis dinámico factorial) a las
CPUE de palangreros Brasileños e índices climáticos. Capitulo 9. In: JC Gutiérrez Estrada, Y Yáñez
(Eds). Nuevas aproximaciones metodológicas para el análisis de pesquerías. ISBN: 978-84-9682655-7.
proceedings)
1. Erzini, K. (Coord.), Veiga, P, Ribeiro, J, Almeida, C, Oliveira, F, Bentes, L, Monteiro, P,
Gonçalves, JMS. 2008. Study of the recreational fisheries in the south Portugal (in portuguese).
Final Report. FCT POCI/MAR/58157/2004. University of Algarve, CCMAR, Faro, 82 pp + Annexes.
2. Erzini, K, Abecasis, D, Bentes, L, Monteiro, P, Oliveira, F, Veiga, P, Gonçalves, JMS. 2008. Sea
bream spatio-temporal dynamics and habitat use in the Ria Formosa lagoon (in portuguese).
Final Report. FCT POCI/BIA-BDE/61949/2004. University of Algarve, CCMAR, Faro, 94 pp.
3. Gonçalves, JMS, Oliveira, F, Coelho, R, Veiga, P, Erzini, K. 2008. Roteiros do mar: região de Vila
Real de St. António. Faro: Câmara Municipal de VRSA & Universidade do Algarve, 110 pp.
4. Gonçalves, JMS, Monteiro, P, Afonso, C, Almeida, C, Oliveira, F, Rangel, M, Ribeiro, J,
Machado, M, Veiga, P, Abecasis, D, Pires, F, Fonseca, L, Erzini, K, Bentes, L. 2008. Cartografia e
caracterização das biocenoses marinhas da Reserva Ecológica Nacional Submarina entre a Galé e
a foz do rio Arade. RENSUB III. Relatório Final CCDR Algarve. Universidade do Algarve, CCMAR,
Faro, 144 pp. + Anexos.
5. Gonçalves, JMS, Rangel, M, Afonso, C, Oliveira, F, Bentes, L, Veiga, P, Monteiro, P. 2008.
Biodiversidade marinha associada à marina de Albufeira. Relatório final. CCMAR, 31 pp.
6. Gonçalves, JMS, Oliveira, F, Coelho, R, Saldanha, H, Veiga, P, Erzini, K. 2008. Projecto
THON.DOC, relatório final. CCMAR, 111 pp.
190
7. Gonçalves, JMS, Rangel, M, Afonso, C, Oliveira, F, Bentes, L, Veiga, P, Monteiro, P. 2008.
Estudo de reabilitação e valorização do troço de costa entre praia D. Ana e a Praia do Canavial
(Lagos) -“Ambiente Marinho”, relat rio final. CCMAR, 58 pp.
8. Gonçalves, JMS, Monteiro, P, Afonso, C, Conduto, T, Almeida, C, Machado, M, Oliveira, F,
Bentes, L, Erzini, K. 2008. Avaliação de Impactes, Medidas de Minimização e Plano de
monitorização de áreas de exploração de inertes subtidais de Albufeira. Relatório
CCMAR/CIMAR Amb & Veritas, 26 pp. + anexos.
monitorização de áreas de exploração de inertes subtidais de Quarteira. Relatório
Bentes, L, Erzini, K. 2008. Caracterização de referência biológica de áreas de exploração de
inertes subtidais de Quarteira. Relatório CCMAR/CIMAR Amb & Veritas, 46 pp. + anexos.
monitorização de áreas de exploração de inertes subtidais de Albufeira. Relatório
MASTER THESES
Almeida, C. 2008. Distribution and shell pattern of hermit crabs in Algarve Coast. Master thesis,
Faculty of Marine Sciences and Environment, University of Algarve.
Batalha, R. 2008. Coexistência e Comportamento das Lagostas de Cabo Verde. Master thesis in
Marine Biology, Faculty of Marine Sciences and Environment, University of the Algarve.
Coxey, MS. 2008. Biological diversity and community structure in vessel reefs in the coast of
Recife (PE) – Brazil. Master thesis, Faculty of Marine Sciences and Environment, University of
Algarve.
Fernandez-Carvalho, J. 2008. Application of underwater video techniques for the
characterization of underwater benthic communities. Master thesis, Faculty of Marine Sciences
and Environment, University of the Algarve.
Ferreira, A. 2008. Lipofuscin concentration versus length as indicators of age in decapods. Using
the Norway lobster (Nephrops norvegicus) as a model. Master thesis in Marine Biology, Faculty
of Marine Sciences and Environment, University of the Algarve.
Mendes, B. 2008. Can reef conservation programs help reduce SCUBA diving damage to coral
reefs? A case study in Thailand. Master thesis in Marine Biology, Faculty of Marine Sciences and
Environment, University of Algarve.
191
Ribeiro, I. 2008. Amálise da distribuição do esforço de pesca e das capturas de palmeta
(Reinhardtius hippoglossoides, Walbaum 1792) capturada pela frota commercial Portuguesa na
área de regulamentação NAFO. Master thesis in Aquaculture and Fisheries, Faculty of Marine
Sciences and Environment, University of Algarve.
International workshop on “Telemetry- theory, concepts and applications”, CCMAR, Faro, 29th
September. 29 Participants.
International workshop on “Acoustic telemetry- Equipments and demonstrations CCMAR”, Faro,
30th September. 29 Participants.
2007-2010. The BIOMARES LIFE project is partly funded (50%) by SECIL, the cement
manufacturer.
2007/2008. Estudo de impacte ambiental da dragagem de inertes na costa continental
portuguesa – Componente biologica Algarve. CIMAR/Amb & Veritas.
2008. Estudo de reabilitação e valorização do troço de costa entre praia D. Ana e a Praia do
Canavial (Lagos) - Ambiente Marinho. Câmara Municipal de Lagos, HB Arquitectura, CCDR
Algarve.
Collaborative publication
-
Fraussen, K, Afonso, CML. 2008. A new Euthria (Gastropoda:Buccinidae) from the Cape
Verde Archipelago. Gloria Maris 47(3): 53-60.
International research collaboration
-
Ocean Tracking Network (http://oceantrackingnetwork.org/): global partnership.
-
BIOMARES LIFE project: USA (NOAA) and Spain (CSIC).
-
Working Group on Habitat Mapping (WGHM) of the ICES.
-
EUNIS habitat classification system: Ifremer (France), Marine Institute (Ireland), JNCC
(UK), AZTI and IEO (Spain).
-
Human Dimensions Research Lab., Texas A&M University (recreational fishing research).
192
-
Florida Program for Shark Research, University of Florida, USA and the Federal Rural
University of Pernambuco in Recife, Brazil.
International meetings and symposia
-
World Congress on Marine Biodiversity, Valencia, 11-14 November.
-
Advances in Tourism Research 2008. University of Aveiro, 26-28 May.
-
5th World Recreational Fishing Conference: “The Angler in the Environment”, Dania
Beach, Florida, 10-13 November.
-
9th Ecological Integration Symposium-Texas A&M University, 28-29 March.
-
VI Meeting of the Brazilian Society for the Study of Elasmobranchs, Fortaleza, Brazil, 2328 November.
-
12th European Elasmobranch Association Meeting, Lisbon, 14-16 November.
-
61st Annual Gulf and Caribbean Fisheries Institute Conference, Guadeloupe, French
West Indies, 10-14 November.
-
ICCAT 2008 Shark Stock Assessments Meeting, Madrid, Spain, 1-5 September.
-
24th American Elasmobranch Association Meeting, Montréal, Canada, 23-28 July.
National meetings and symposia
-
Semana do Mar, Faro, 22 September.
-
III Encontro de Pós-graduação em Ciências Biológicas. University of Algarve, 3-5
September.
Visiting scientists
-
Jacques Populus (IFREMER), José Luis Sanz (IEO), Jesus Rivera (IEO), Natalie Coltman
(JNCC), Ibon Galparsoro (AZTI) and David Connor (JNCC) 1st Mesh-Atlantic preparation
meeting.
Graduate training
-
Coordination and teaching: Erasmus Mundus Masters in Marine Biodiversity and
Conservation and the International Masters in Aquaculture and Fisheries.
-
Teaching: Masters in Marine Resources and Coastal Management in Cabo Verde:
Conservation and Management, Statistical Analysis, and Modeling in Fisheries; Masters
in Aquaculture and Fisheries of the University of Cádiz.
193
With the objective of contributing to the management of the underwater (from 0 to 30 m
depth) component of the National Ecological Reserve, the subtidal marine communities of the
Algarve are being mapped. In 2008 the area between Galé and the Arade estuary was
completed (Cartography and characterization of the marine communities off the National
Underwater Ecological Reserve between Galé and the Arade river (RENSUB III). This study was
funded by the regional administration (Comissão de Coordenação e Desenvolvimento Regional
do Algarve, CCDR Algarve) and is intended to help implement a spatial planning of the marine
environment which could minimize potential conflicts between the variety of activities taking
place in this particular coastal zone.
The BIOMARES LIFE project (2007-2010), is partly funded (50%) by SECIL, the cement
manufacturer.
2007/2008. Estudo territorial e do meio ambiente marinho, ao abrigo do projecto comunitário
“Thon.doc”. Câmara Municipal de Vila Real de Santo Ant nio.
8e. Future research
1. Objectives
Marine Protected Areas (MPA)
-
Within the context of the BIOMARES project (2007-2010), the group will focus on
evaluating the effects of different levels of protection within the Arrábida marine park
(total, partial and complementary). Regular monitoring using a variety of gears is being
carried out. Indicators will be calculated from these data (e.g. density of key species,
mean sizes). Acoustic telemetry will be used to study spillover or the contribution of the
MPA to the adjacent non-protected areas. Key species to study include several sea
breams, soles, bass and cuttlefish. The role of herbivory within the MPA will also be
studied, focusing on the biology and ecology of the dominant herbivore fish species,
Sarpa salpa.
Spatio-temporal dynamics and habitat use
-
194
In addition to the telemetry studies in the MPA, the group will continue acoustic
telemetry studies in Algarve coastal waters, with emphasis on artificial reef related work
in collaboration with IPIMAR. Specifically, the goals are to evaluate the use of artificial
reefs (the largest area in European waters) by wild and cultured (stocked) sea breams.
Through the global Ocean Tracking Network project, the group will participate in the
deployment of arrays of hydrophones across the Strait of Gibraltar and the continental
shelves of southern Portugal and north-western Morocco. These arrays will eventually
allow the monitoring of movements of highly migratory species. A PhD grant has been
awarded to D Abecasis, who will use acoustic telemetry to study spatio-temporal
dynamics and habitat use of key species in the Arrábida marine park.
Seabed classification, mapping and essential fish habitat
-
In the next few years the group will finish mapping the entire Algarve coastal waters to a
depth of 30m using divers, video camera mounted on a towed sledge and beam trawl.
The data is being implemented in GIS and will be used develop indicators and for
modeling (e.g. GAMs), allowing the identification of biodiversity hotspots and essential
fish habitat. The group has been invited to participate in a major pan-European proposal
focusing on sea bed mapping and biodiversity.
Monitoring of key habitats
-
In addition to the monitoring in the Arrábida MPA, the group will continue their longterm annual monitoring programs of the ichthyofauna of the Ria Formosa lagoon (since
2001) and the Arade River estuary (since 2003).
Sport fishing
-
Having completed a study of shore-based rod and line sport fishing, along 250 km of
coastline from Sines to Vila Real de Stº António, the group will now focus on boat-based
sport fishing and underwater spear fishing. Experimental fishing trials within a large
earth tank will also take place to obtain, for the first time, direct estimates of hook
selectivity.
Fisheries management, population dynamics and conservation
-
The group will continue work contributing to ensuring the sustainability of Portuguese
fisheries. In this context, ongoing studies are evaluating of alternative, less damaging
fishing gears, in specific fisheries. Simulation and modeling studies will also be used to
evaluate different management scenarios. Ongoing bio-economic studies will also
contribute to providing the basis for improved management and conservation of
fisheries resources. Population dynamics and demographic analysis of top predators,
especially sharks, are also a priority.
2.

Current funding
71.710 EUR, INAG, Avaliação do Estado Ecológico das Massas de Água Costeiras e de Transição e
do Potencial Ecológico das Massas de Água Fortemente Modificadas, 2009-2010.
2.364.438 EUR (254.388 EUR for group), LIFE Program (EU) and Secil Restoration and
Management of Biodiversity in the Marine Park Site Arrábida-Espiche, LIFE Project - PTCON0010,
2007-2010.
195
124.916 EUR, ARH Algarve, RENSUB IV, Cartography and characterization of the marine
communities off the National Underwater Ecological Reserve between the Arade River and
Ponta da Piedade, 2009-2011.
Ocean Tracking Network (OTN), Canada Foundation for Innovation, 2007-2011. $35.000.000.
(unspecified amount - still being negotiated).

199.470 EUR, PROMAR, eixo 3, DGPA, SPEARFISHING - Avaliação da pesca submarina em
Portugal continental.
984.714 EUR, UE-ACP Science and Technology Programme, TOOLFISH - Developing tools for
fishery and marine priority habitat’s management: biodiversity and fisheries.
118.050 EUR, PROMAR, eixo 3, DGPA, PESCABORDO - Estudo da pesca recreativa embarcada na
costa sul de Portugal.
-
FCT
141.588 EUR, Habitat use and adaptive management of a marine protected area.
198.192 EUR, REEFQUEST - Mapping marine priority reef habitats: biodiversity and fisheries.
114.939 EUR, Recreational boat fishing: impacts on living resources and implications for
sustainability and management.
173.662 EUR, Survivorship, long term movements, habitat preferences and impacts of big game
fishing on highly migratory sharks in the Algarve.
199.620 EUR (123.300 EUR for group), Pelagic sharks in the eastern Atlantic Ocean: fisheries, life
history, migrations, population dynamics and demographics.
196
Fátima Abrantes (represented by João Coimbra)
The group which develops its activity at the Department of Marine Geology of INETI (future
LNEG) received funded from several sources: Ministry of Economy and Innovation (MEI), FCT
(national projects and Eurocores ESF), pluriannual funds through CIMAR and other funds for
participation in meetings or short stays at foreign laboratories. In summary, the funds spent in
2008:
-
MEI
Human resources: 455.220,81 EUR.
Fellowships: 33.730,91 EUR.
Others: 108.604,82 EUR.
-
CIMAR
Human resources: 39.612,00 EUR.
Others: 20.000,00 EUR.
-
FCT
National projectos: 231.000,96 EUR.
Fellowships: 197.650,00 EUR.
-
Other sources
Services: 9.595,11 EUR.
197
Grants: 18.834,35 EUR (Gulbenkian, ESF, FLAD).
1. Objectives
This report lists the results obtained during the year 2008 at the Marine Geology Department of
INETI, within the main research programme INGMAR - Investigação em Geologia Marinha.
Within this progamme, which counted with 80 K EUR of Institutional funds (PIDDAC) and that
represent 0.02% of the total funds 99.8% of which come from the 13 research projects in
execution: 7 funded by the National Science Foundation - FCT (SADOGEOROB, EVO-TROIA,
CROSTAS, CANHÕES, CUPEX, PORTO, HOLOCLIMA), one project funded as part of the ESF
EUROCORES / EUROMARGINS (SEDPORT) and one service project was finalized (AREIAS). Besides
this direct project funding, quite a value of K EUR has also been attributed to DGM personnel,
through fellowships directly paid by the National Science Foundation (33 in a total of 44
technicians and researchers), as well as by several other national and international institutions
through special funds to many fellows to attend meetings and workshops, or visit other
institutions.
In terms of new projects, 5 new proposals were approved in 2008, three of which in the areas
associated to CIMAR, FCT (INTER-TRACE and MELT KP5) and one Interreg Portugal-Galiza
Cooperation (NATURA MINHO). Furthermore, the group is also participating in the project
“Antarctic seaways and global change – LO ANT” funded by the Spanish SCIC and leaded by
Luís Somoza from the Instituto Geológico y Minero (Spain).
The high-resolution multiproxy studies on Portuguese Margin sediments shows that Sea Surface
Temperature (SST) co-varies with Northern Hemisphere (NH) T and solar insolation. Rainfall &
NAO are anti-correlated but flooding events occured during climate deterioration and solar
activity transitions. At AD1850, water column stratified and primary production decreased.
The investigation of turbidite deposits between the Cascais and Lisbon-Setúbal canyons show
that frequency of turbidites increased during Heinrich & D-O events, culminating at maximum T
and sea level gradients between Greenland Stadials & Antarctic warming events, and when the
Atlantic Meridional Overturning Circulation re-starts. The Portuguese Margin Slope instability is
thus induced by 15-30m sea level variation, rapid deep and intermediate water circulation
changes.
The studies of “Samudra Manthana” an ancient tale narrated in “Vishnu Purana”, a 2nd century
BC Sanskrit text, reveal the causes for South Asian cultural-changes. A prolonged draught due to
low precipitation / abrupt cooling around 8.2 kyr BP, forced the agrarian societies to explore
new strategy to ensure security of food and other essentials. Thus, cattle were domesticated,
and as it assured long-term food and energy, it became a form of wealth and a deity.
Correlations between marine and terrestrial climatic indicators depict a complex pattern of
Heinrich events 4, 2 & 1 in the western Iberian region. Low IRD deposition, extreme oceanic and
198
atmospheric cold condition followed by relatively wet conditions in the eastern mid- and
subtropical latitudes are observed.
Studies on sensitivity of Gas Hydrates in Gulf of Cadiz to oceanographic changes suggest that
seafloor T increase, linked with the last warming transition and/or beginning of the
Mediterranean Outflow, can efficiently trigger episodes of dissociation of potential gas hydrates
which would release methane rich fluids to shallow sediments, ocean and even atmosphere.
A joint mission to Mozambique, with Geologists from the Moçambique Geological Survey,
initiated the process of Geological mapping of the Bilene Island coasts.
New Proposals
Accepted for funding: Cooperation Portugal-Galiza 2008; Natura Minho
Decision waited: EU Atlantic Space 2008; CLIVOCEAN; QREN Algarve, Coast Evolution in the
Eastern Algarve.
8d. Productivity
1. Abrantes, F, et al. 2008. Tsunamis Sedimentological Record on Shallow Shelf areas: The cases
of 1969 AD and 1755 AD on the Portuguese Shelf off Lisbon. Marine Geology 249: 283-293.
IF=2.100.
2. Salgueiro, E, et al. 2008. Planktonic Foraminifera from Modern Sediments Reflect Upwelling
Patterns off Iberia: Insights from a Regional Transfer Function. Marine Micropaleontology 66(34): 135-164. IF=1.954.
3. Muiños, SB, et al. 2008. New constraints on the Pb and Nd isotopic evolution of NE Atlantic
water masses. Geochemistry, Geophysics, Geosystems 9, Q02007, doi:10.1029/2007GC001766.
IF=2.979.
4. Mil-Homens, M. et al. 2008. Historical trends in Hg, Pb and Zn sedimentation in the central
shelf area of Portugal. Journal of Iberian Geology 34(2): 272-286. Special Issue dedicated to the
Human Impact on the Coast.
5. Ávila, SP. et al. 2008. Local disappearance of bivalves in the Azores during the last glaciation.
Journal of Quaternary Science 23(8): 777-785. IF=2.976.
6. Mitchell, NC, et al. 2008. Lava penetrating water: submarine lava flows around the coasts of
Pico
Island,
Azores.
Geochemistry,
Geophysics,
Geosystems
9,
Q03024,
doi:10.1029/2007GC001725. IF=2.979.
7. Dias, AS., et al. 2008. Geochemistry of a sediment push-core from the Lucky Strike
hydrothermal field, Mid-Atlantic Ridge. Chemical Geology 247(3-4): 339-351. IF=3.531.
199
8. Sánchez Goñi, MF, et al. 2008. Evidence for major sources of CH4 in northern mid-low
latitudes during the last glacial. Quaternary Science Reviews 27: 1136-1151. IF=3.693.
9. Nuzzo, M. et al. 2008. Shallow Microbial Recycling of Deep-Sourced Carbon in Gulf of Cadiz
Mud Volcanoes. Geomicrobiology Journal 25(6): 283-295. IF=1.495.
10. Aranda da Silva, A, Gooday, AJ. 2008. Large organic-walled Protista (Gromia) in the Arabian
Sea: density, diversity, distribution and ecology. Deep-sea Research Part II. (in press).
doi:10.1016/j.dsr2.2008.10.001. IF=1.411.
11. Gooday, AJ, et al. 2008. Faunal responses to oxygen gradients on the Pakistan margin: a
comparison of foraminifera, macrofauna and megafauna. Deep-sea Research Part II. (in press).
doi:10.1016/j.dsr2.2008.10.003. IF=1.411.
proceedings)
1. Abrantes, F, et al. 2008. Geophysical Research Abstracts, Vol. 10, EGU2008-A-04305,
European Geosciences Union General Assembly, Austria.
2. Brito, P, et al. 2008. Late quaternary coastline variations between Tróia and Cape Espichel
from new geological and geophysical data. Proceedings of PoCoast Seminar on Coastal
Research, Porto, Portugal.
3. Kulhanek, DK, et al. 2008. Centennial-scale nannoplankton productivity changes in the Midlatitude North Atlantic during marine isotope stages 11-12: evidence from IODP Site 1313.
American Geophysical Union Fall Meeting, USA. Abstract #PP11B-1395.
4. Lopes, C, Abrantes, F, Mix, AC. 2008. Diatom transfer functions for sea surface temperature
and primary productivity in upwelling areas: the CUPEX project. American Geophysical Union
Fall Meeting, USA. Abstract #PP41B-1459.
5. Magalhães, VH, et al. 2008. Effect of climate variations on gas hydrate stability conditions at
fluid escape structures in the Gulf of Cadiz. American Geophysical Union Fall Meeting, USA.
Abstract #U23D-0090.
6. Magalhães, V, et al. 2008. Geophysical Research Abstracts, Vol. 10, EGU2008-A-04097,
European Geosciences Union General Assembly, Austria.
7. Mil-Homens, M, et al. 2008. Geophysical Research Abstracts, Vol. 10, EGU2008-A-08342,
European Geosciences General Assembly, Austria.
8. Patton, GM, et al. 2008. Mg/Ca Paleothermometry in the Central Gulf of Cadiz During Heinrich
Events. American Geophysical Union Fall Meeting, USA. Abstract #PP11A-1368.
9. Quartau, R, et al. 2008. Geophysical Research Abstracts, Vol. 10, A-04021, European
Geosciences Union General Assembly, Austria.
200
10. Salgueiro, E, et al. 2008. Upper Water Column Structure during Marine Isotope Stage 11.3
and the Holocene on the Portuguese Margin. American Geophysical Union Fall Meeting, USA.
Abstract #PP33C-1563.
11. Voelker, A. et al. 2008. Tracing the Influence of Mediterranean Outflow Waters on the Middepth Portuguese Margin between Marine Isotope Stages 9 and 13. American Geophysical
Union Fall Meeting, USA. Abstract #PP33C-1566.
proceedings)
1. Ferraz, M, Rebêlo, L, Brito, P. 2008. Identification and characterization of the Tróia Península
Northern part dune fields. Executive Summary Research Highlights and Publications 2007.
CIMAR 2008. Porto. pp. 6-7.
MASTER THESES
Costa, A. 2008. Variation of Hemipelagic Sedimentation in the Submarine Cascais Canyon and
Adjacent Slopes for the Last 150 Years. University of Lisbon.
Ferraz, M. 2008. Caracterização das Dunas e Campos Dunares da Parte Norte da Península de
Tróia. University of Lisbon.
PhD THESES
Stronowski, Z. 2008. A High - Resolution Climate Study on the Norwegian & Iberian Shelves
During the Late Holocene: A Diatom Perspective. PhD thesis, University of Plymouth.
As part of the European programme Leonardo da Vinci several students have done 6-month
training projects at DGM:
-
Elena Amigoni and Lucia Zanetti (September 2007 – March 2008), University of Génova.
201
Participation in Cruizes
-
Microsystems on board the French ship Marion Dufresne (Brest - 15 July, Algeciras - 24
July 2008). Participation of A Voelker (PI), P Freitas, A Rebotim, C Santos, L Matos.
-
JC027 on board RV James Cook – landslides and gravity flow geohazards, 3 August - 5
September 2008, SOC, UK. Participation of L Baptista.
-
NEAREST-SEIS on borad BIO Hespérides, Cadiz Gulf. Participation João Duarte.
-
POS377 on board FS. Poseidon, 5-22 December 2008 from Las Palmas to Las Palmas.
Participation of A Rebotim.
Visits to Other Laboratories
-
Susana Muiños, USGS Menlo Park, April to August.
-
João Duarte, Toronto University, GeoTec Lab, January.
-
Isabelle Gil, WHOI, April to August.
-
Filipa Naugnton, WHOI, August; GEOTOP, Canada, September to December
-
Antje Voelker, University of Chicago, USA, December.
-
Gabriella Carrara, ISMAR – BO, CNR, Italy, December 2008 and January 2009.
-
Rui Quartau, University of Manchester, December.
8e. Future research
1. Objectives
Reaching international level in Geological Oceanography research depends on the existence of
qualified staff. As such DGM will continue contributing to the education of a new generation of
researchers, through PhD plans to be executed at DGM but supervised in collaboration with
internationally recognized researchers from Excellent Institutions in the properly selected
research area.
Another important objective is to gain access to laboratory equipment, so that we can have the
capacity to produce scientific results at the level of our international partners, as well as be
competitive relatively to European/International funds, and, maintain and attract to Portugal
the best quality researchers.
202
D M’s Research Plan is built around four main scientific areas: Paleoceanography and
Environment, Geology and Resources, Natural Hazards, and Coastal Geology. Besides, we will try
to expand the area of Information and Technology.
Research within Paleoceanography and Environment is centered in the reconstruction of climate
and oceanographic conditions at scales that vary from annual to tectonic (million years), on the
basis of the study of geologic records. A theme of major importance to assess future climate
conditions, since the instrumental record is much too short to allow the separation between
natural variability and the anthropogenic impact.
Most of the work is to be developed on the Portuguese margin/waters, but will certainly include
other areas of the ocean, in particular the five most important coastal upwelling regions.
Regions that due to their high oceanic productivity play a crucial role in modulating the flux of
CO2 between the ocean and the atmosphere being one of the main mechanisms controlling
atmospheric pCO2 and regulating climate. Besides we will also continue centering some of our
attention on the climate reconstruction of the Holocene and Marine Isotopic Stage MIS 11. Two
recent warm periods that may constitute an important source of information for future climate
modelation. Furthermore, we will focus on the needs for the development of new proxies,
always necessary to better understand past climate variability and its forcing processes.
In terms of environment we will follow the investigation on the distribution and temporal
variability of polluting elements brought to the Portuguese estuaries and shelf environments.
Coastal Geology will follow up the studies related to the understanding the geologic evolution of
coastal areas, including dune dynamics, and causing littoral processes. Basic knowledge is
necessary to a sustainable utilization of these heavily populated and environmentally sensitive
regions, keeping in consideration also a possible increase in storm frequency and intensity as
well as sea level rise associated with global warming.
Information and Technology concerns the development of geographic information systems
(GIS), databases, and scientific visualization techniques.
To consolidate the international cooperation, to participate in an innovative educational
program at the European level, to contribute for the transference of knowledge about the
ocean, as well as its importance for the political and public question of how to be prepared to
survive possible important climate changes in the near future, are other of the DGM objectives.
2.

Current funding
Summary of the funds attributed (MEI) and planned for 2009:
-
Execution at INETI
41.007,68 EUR, SANDEX - Extracção de areias na plataforma continental portuguesa:
impactos e evolução morfodinâmica.
95.262 EUR, AMOCINT, EUROMARC/002/2007.
203
185.000 EUR, SEDPORT - Sedimentation processes on the Portuguese margin: the role of
continental climate, ocean circulation, sea level and neotectonics.
39.964,00 EUR, CANHKES - Transporte de sedimentos nos canhões submarinos de
Setúbal e Lisboa.
61.200 EUR, CROSTAS - Crostas de ferromanganês do Atlántico Nordeste:
potencialidades como recurso e importância paleoceanográfica.
35.651,93 EUR, EVO-TROIA - Evolução da Peninsula de Troia: Morfodinâmica dos
sistemas dunares e da linha de costa durante o final do Quaternário.
25.415,21 EUR, SADOGEOROB - Variações da linha de costa meotectónica e evolução do
delta submarino do Sado durante o Quaternário: uma abordagem integrando a geologia
e veiculos robóticos submarinos.
11.054 EUR, HOLOCLIMA - Registo climático holocénico em tufos calcários e sedimentos
lacustres associados.
50.000 EUR, GeoSeas - Pan European infrastructure for management of marine and
ocean geological and geophysical data.
-
Execution at CIMAR
13.046,22 EUR, PDCT/MAR/56963/2004.
10.117,80 EUR, PDCT/MAR/58282/2004, PORTO.
165.329,36 EUR, FCT PTDC/CLI/70772/2006, INTER-TRACE – Rapid changes in
interglacial surface and deep-water properties in the North Atlantic.
175.163 EUR, FCT PTDC/MAR/65197/2006, MELT KP5 – Mantle melting and crust
production in the mid-atlantic ridge under the influence of Azores hotspot: a case study
at KP-5 segment.
106.280 EUR, INTERREG IV POCTEP MINHO-Natura, Valorização dos recursos naturais da
bacia hidrográfica do Minho.

Pending funding
FCT PTDC/AAC-AMB/108449/2008, MONA.
FCT PTDC/AAC-CLI/100157/2008.
204
Hydrobiology
Hydrobiology
Adriano Agostinho Donas Bôto Bordalo e Sá
-
-
FCT Projects
8.174,80 EUR, PTDC/AMB/64441/2006.
1.634,91 EUR, POCTI/CTA/48386/2002.
ERA-AMPERA/0003/2007, 2008-2011, 30.000 EUR.
-
FCT Scholarships
2.750 EUR, Catarina Teixeira, SFRH/BD/22317/2005.
-
FLAD grants
1.500 EUR, Ana Machado, FLAD Grant Program.
-
Others
500 EUR, University of Porto. Escola de Ciências da Vida e da Saúde. Summer course.
250 EUR, University of Porto, Júnior University.
205
1. Objectives
The activities within the Lab are spread over three main areas: (i) research; (ii) consulting; and
(iii) outreach. The Lab aims to contribute to the aquatic research from a multidisciplinary point
of view, dealing from water quality monitoring to modelling, from early life stages of fish to
denitrification and global warming and epidemiology. Research is carried out in temperate
(Portugal) and tropical environments (Thailand – SE Asia, Guinea-Bissau– W Africa, S. Tomé and
Principe – W Africa). The main research areas are (a) monitoring estuarine water quality in
terms of physical (conductivity, salinity, temperature, turbidity), chemical (dissolved oxygen,
inorganic carbon, pH, nitrate, nitrite, ammonium, phosphate, silicate) and biological (bacteria,
viruses, fecal indicators, primary production) parameters, (b) estuarine primary productivity, (c)
nitrogen cycle and global warming, (d) anthropogenic impact in estuarine communities, (e)
phytoremediation and bioremediation, (f) estuarine fish assemblages, with special emphasis to
the estuarine nursery role for early life stages of fishes (g) watershed restoration, (h) ecological
modelling, (i) tropical water quality and cholera, (j) water management and public health.
-
Monitoring estuarine water quality: research in the River Douro estuary involving
monthly water column (since 1985) key environmental data.
-
Estuarine primary productivity: research in the River Douro estuary has been performed
in the water column, intertidal sediments and hard surfaces.
-
Nitrogen cycle and global warming: research has been carried out in the water,
sediments and hard surfaces in several estuaries in Portugal (Douro, Cávado, Sado and
Ave) as well as in Bangpakong estuary, Thailand. Nitrogen fixation, nitrification,
denitrification, annamox and nitrous oxide production, a powerful green-house gas
were evaluated. In addition, the structure of the microbial populations involved in
nitrification and denitrification are also studied.
-
Anthropogenic impact on estuarine communities: research performed in Douro and
Lima estuaries in order to understand the effect of heavy metals and other pollutants on
macrobenthic and microbial communities and on the denitrification process.
-
Phytoremediation and bioremediation: the role of the interaction between plants and
microorganisms in the removal of heavy metals and other pollutants from sediments
has been studied in several estuaries in Portugal (Douro, Cávado and Sado).
-
Estuarine fish assemblages: research conducted in the Lima River estuary to understand
the dynamics of fishes, focusing on the potential nursery role of estuaries and ultimately
assess the carrying capacity of these ecosystems for fish species, some with high
economical important such as sardines and flatfishes.
-
Watershed restoration: using the River Febros watershed (a tributary of the Douro) as a
model, different tools for water quality, sediment quality and river bank quality have
been employed.
206
-
Ecological modeling: by means of an object-oriented model several key parameters,
including primary production, have been modelled in the River Douro estuary.
-
Tropical water quality, cholera and public health: research done in Thailand, GuineaBissau and S. Tomé & Principe. In Thailand the water quality of the Bangpakong River
watershed and subtidal sediments has been carried out. In Guinea-Bissau, water quality
and its relation to disease including cholera has been evaluated and sustainable
measures do increase water quality have been designed. In S. Tomé & Principe the
water quality in the island of S. Tomé was evaluated for the first time.
8d. Productivity
maritimus and Scirpus maritimus) as sources of strong Cu-complexing ligands in estuarine
environments. Estuarine Coastal and Shelf Science 77: 104-112. IF=2.072.
2. Magalhães, C, Bano, N, Wiebe, W, Bordalo, AA, Hollibaugh, J. 2008. Dynamics of nitrous oxide
reductase genes (nosZ) in intertidal rocky biofilms and sediments of the Douro River estuary
(Portugal), and their relation to N-biogeochemistry. Microbial Ecology 55: 259-269. IF=2.885.
3. Magalhães, C, Teixeira, C, Teixeira, R, Machado, A, Azevedo, I, Bordalo, AA. 2008. Dissolved
organic carbon and nitrogen dynamics in the Douro River estuary, Portugal. Ciencias Marinas 34:
271-282. IF=1.038.
4. Azevedo, I, Duarte, PM, Bordalo, AA. 2008. Understanding spatial and temporal dynamics of
key environmental characteristics in a mesotidal Atlantic estuary (Douro, NW Portugal).
Estuarine Coastal and Shelf Science 76: 620-633. IF=2.072.
5. Almeida, CMR, Mucha AP, Bordalo, AA, Vasconcelos, MTSD. 2008. Influence of a Salt Marsh
Plant (Halimione portulacoides) on the concentrations and potential mobility of metals in
sediments. Science of the Total Environment 403: 188-195. IF=2.579.
6. Almeida, CMR, Mucha AP, Delgado, MFC, Caçador, IM, Bordalo, AA, Vasconcelos, MTSD. 2008.
Can PAHs influence Cu accumulation by salt marsh plants? Marine Environmental Research 66:
311-318. IF=2.032.
proceedings)
proceedings)
207
PhD THESES
estuarine water quality and primary production. PhD thesis in Aquatic Sciences, Instituto de
Ciências Biomédicas Abel Salazar, University of Porto. Supervisor AA Bordalo, Co-supervisor:
Pedro Duarte (University Fernando Pessoa).
Águas do Porto - Avaliação da qualidade das praias na frente marítima do Porto.
United Kingdom
-
IECS, University of Hull: Prof. M. Elliott. Derive and test a quantitative indicator of
ecosystem functioning and health for use in management plans and the future policies
for the estuarine management.
Spain
-
GEOMA, University of Vigo: Prof. Ana Bernabeu. Buried oil in the intertidal beach zone:
coupling between beach morphodynamic, natural degradation, forcing mechanisms and
biological activity.
France
-
GLADYS, University of Montpellier 2: Prof. Frédéric Bouchette. Buried oil in the intertidal
beach zone: coupling between beach morphodynamic, natural degradation, forcing
mechanisms and biological activity.
United States of America
-
208
Department of Marine Science, UGA: Prof. W Wiebe, Prof. J Hollibaugh, Prof. S Joye.
Structure and function of microbial communities; biogeochemical cycling of nutrients.
-
RSMAS, University of Miami: Prof. R Cowen, Prof. C Paris. Fisheries oceanography and
larval ecology.
-
Dauphin Island Sea Lab, University of South Alabama: Prof. R Kiene. Cycling of organic
sulfur in aquatic systems.
-
University of Maryland: Prof. Rita Colwell. Cholera dynamics in aquatic systems.
New Zealand
-
University of Waikato: Prof. C Cary, Prof. IR McDonald. Microbial diversity along
latitudinal gradient of Antarctica.
-
Post-Doc programme: C Magalhães.
Guinea-Bissau
-
Research with personal funds (AA Bordalo). Variability of well water quality dry/wet
seasons, outbreak of intestinal diseases and cholera.
S.Tomé and Principe
-
Research with personal funds (AA Bordalo). Variability of freshwater quality for
domestic use.
FCT, PTDC/AMB/64441/2006, 2008-2010. AQUASENSE – Development of miniaturised flow
techniques for investigating dynamic environmental systems: river, estuarine and coastal
waters.
FCT, POCTI/CTA/48386/2002, 2005-2008. Rhizosphere Biogeochemistry and its Relevance for
Endpoints and Phytoremediation.
University of Porto. Escola de Ciências da Vida e da Saúde. Summer course 2008.
8e. Future research
1. Objectives
The Lab intends to pursuit its multidisciplinary approach, with special emphasis in the following
research areas: estuarine and coastal dynamics, watershed management, Water Framework
Directive implementation, microbial diversity and function, nitrogen and sulphur cycles and
global warming, bioremediation, cholera and tropical water quality and public health.
209
Since 1985, research has been focussed on the Douro estuary and the coastal zone of Porto.
Progressively, research has expanded into other Portuguese estuaries (Lima, Cavado, Ave, Leça).
In 2008, the evaluation of freshwater quality in S. Tomé Island (W Africa) was carried out
together with the continuation of the work in Guinea-Bissau. Besides basic knowledge of each
system, the attention of the researchers has been devoted to processes and functions and their
implications to the health of the ecosystems as well as to human health.
The current management of aquatic ecosystems relies on a good knowledge of their structure
and functioning and of the way in which the habitat status, use, loss and gain has been
influenced by human activities. The EU Water Framework Directive requires the classification of
the ecological status of all water bodies. Thus, research in this area will deal with development
of tools that allow the assessment of ecological status of these ecosystems, the evaluation of its
response to natural and man-induced stressors and the design of mitigation measures to fulfil
legislation requirements. Those tools will include the design of conceptual models of ecosystems
function, development of new environmental indicators and methodologies for health
assessment and restoration of small watersheds.
Specifically, ongoing research in nitrogen cycle will proceed in order to investigate the role of
denitrification, anammox, dissimilatory nitrate reduction to ammonium and nitrification as fates
for nitrogen transformations in estuarine sediments. Another goal will be the evaluation of new
interactions between organic sulfur degradation compounds and the last step of denitrification,
including processes (involved in global warming) and microbial communities. These studies
represent a new perspective of the interaction between marine nitrogen and sulfur cycles,
giving a new understanding about nitrous oxide production in the marine environment. The
dynamics of microbial communities and of key nitrogen processes with main relevance to
potential release of N2O (with high impact in the global warming), in tropical estuaries will also
be addressed. In future, we also intend to extend studies to the Antarctica environments, in
order to understand how microbial diversity and function changes along latitudinal gradients.
Another challenging area is bioremediation. The studies will be carried out in coastal
ecosystems, in order to develop methodologies for the cleanup of oil contaminated sediments,
based on the stimulation of the degradation processes of microbial communities. These also
involve the isolation of autochthonic hydrocarbon biodegrading microorganism, not genetically
modified.
Finally, the research in Guinea-Bissau which pursuits the amelioration of the living conditions of
the population including the decrease of child mortality (presently 204/1,000 births against
3/1,000 births in Portugal), through the implementation of simple and sustainable measures
based in the results of the ongoing studies will be monitored on site. Similar were carried out in
S. Tomé and Principe islands also in W Africa.
2.

Current funding
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25.800 EUR, FCT PTDC/AMB/64441/2006, AQUASENSE – Development of miniaturised flow
techniques for investigating dynamic environmental systems: river, estuarine and coastal
waters, 2008-2010. Partners: ESB/UCP, CIIMAR.
30.000 EUR, FCT - AMPERA Joint Management Committee (ERA-AMPERA/0003/2007),
OILDEBEACH - Buried oil in the intertidal beach zone: coupling between beach morphodynamic,
natural degradation, forcing mechanisms and biological activity, 2008-2011. Partners: Univ.
Vigo/GEOMA (Galicia/ES), CIIMAR (PT), Univ. Montpellier II/GLADYS (FR), ICBAS (PT).

Pending funding
137.256 EUR, FCT, PTDC/MAR/099140/2008, PHYTOBIO - Phytoremediation and bioremediation
of contaminants in salt marshes: plant–microorganisms interactions. PI: AP Mucha.
134.256 EUR, FCT PTDC/AAC-AMB/099038/2008, MEPAMOX – Interference of Metals and PAHs
in Nitrate Removal Biological Processes: Denitrification vs Anammox. PI: C Magalhães.
133.548 EUR, FCT PTDC/BIA-MIC/099304/2008AMOX - Relative importance of archaeal and
bacterial ammonia oxidizers in estuarine ecosystem function: role of environmental controls. PI:
C Magalhães.
111.906 EUR, FCT PTDC/MAR/098914/2008NITROSUL - Novel interaction between marine
biogeochemical nitrogen and sulfur cycles: characterization and ecological implications. PI: C
Magalhães.
122.250 EUR, FCT PTDC/MAR/100252/2008ENVINURSE - Development of an environmental
integrative indicator of the estuarine fish nursery function. PI: S Ramos.
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Marine Plant Ecology Research Group (ALGAE)
Marine Plant Ecology Research Group (ALGAE)
Rui Orlando Pimenta Santos
113.000 EUR, FP6-2004-SME-COOP, Envirophyte, 2006-2008.
100.000 EUR, R.C. Hidromod/Electricidade de Portugal, 2006-2008.
95.000 EUR, FCT POCTI/MAR/58172/2004, 2005-2008.
73.800 EUR, FCT POCI/MAR/58427/2004, 2005-2008.
85.500 EUR, FCT POCTI/MAR/55377/2004, 2005-2008.
81.000 EUR, FCT POCTI/MAR/56956/2004, 2005-2008.
120.000 EUR, BISHOP PTDC/CTE-GEX/70448/2006, 2007-2010.
9.100 EUR, Ministerio Educación y Ciencia (Spain), CGL2006-03576/BOS, 2007-2011.
1. Objectives
1. Metabolism of coastal systems:
This research line aimed the role of macrophyte photosynthetic performance, particularly
seagrasses and red calcifying algae (mäerl), in the production and carbon budget of the whole
communities. The underlying mechanisms involved in the seagrass meadows production were
investigated in particular their photosynthetic and calcification processes. In situ field
measurements were done in southern Portugal and northern Spain and in the laboratory. In
particular, we determined the influence of light and temperature in those processes.
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2. Human impacts on coastal Systems:
This research line focused on the effects of the human-related disturbances on the costal
ecosystems of southern Portugal. Our aim was to contribute with sound scientific inputs to the
conservation and management of these systems. In particular, we investigated the effects of
urban effluents and sedimentation disturbances on the population ecology and biology of
seagrasses and the biological and the population ecology traits of one of the most invasive
marine algae, Sargassum muticum.
3. Economic valorization of macrophytes:
3.1. Research was focused to interface basic algal physiology with industrial applications,
providing the basis for novel uses of seaweed in benefit of animal mariculture. The use of the
seaweeds’ stress physiology for optimization of both algal growth and overproduction of
valuable secondary metabolites was investigated.
3.2. Research was also conducted on the use of salt-marsh plants as biofilters for fishfarm
effluents. Constructed wetlands based on Chenopodiaceae species with economical value were
used to evaluate both the biofiltration potential and the plant biomass productivity and quality.
4. Effects of global change on the Portuguese marine flora:
The objective was to evaluate the long-term changes in the benthic marine flora by comparing
the present flora with that described by Ardré in the 1960´s. Range shifts of species, variations in
their abundance or new introductions were analyzed to test the hypotheses that the richness
and abundance of warm-water species has increased, as opposed to cold-water species. We
found that the long-term changes in the Portuguese marine flora are in accordance with the
expected under sea surface temperature warming.
In 2008 we produced 7 scientific publications, concluded 4 national and 1 European projects and
1 PhD and 2 Masters thesis.
We concluded the first comprehensive evaluation of the carbon budget of the intertidal area of
Ria Formosa coastal lagoon, from monthly data on the community metabolism of the seagrass
Zostera noltii and the halophyte Spartina maritima. Our data show that the net community
metabolism of both species is mainly conditioned by temperature, whose oscillations are a more
important driver than irradiance for the net carbon budget, mostly due to its pronounced
influence in respiration rates.
We related the local colonization and establishment of the invasive brown seaweed Sargassum
muticum to the semi-lunar periodicity of gamete release. Population growth rates increased and
stabilized as the species became established and dominant. Multiple food choice experiments
showed that meso-herbivores in Portugal prefer native seaweeds as food sources rather than
the invader. S. muticum also benefits from high growth rates and relatively low grazing pressure
which increase its competitiveness.
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We have identified the major algal-bloom that developed in the coast of Quindao during the
Olympic Games.
We contributed with sound scientific inputs to the management of coastal systems by describing
the impacts of sediment burial and erosion on seagrasses in general and revealed the impact of
the urban effluents on a seagrass species in particular.
We formalized the implementation of the two first seagrass monitoring sites in Portugal under
the global monitoring “SeagrassNet” program to be launch in 2009 in Ria Formosa lagoon.
We concluded about the suitability of the tetrasporophytes of Bonnemaisoniaceae for inland
integrated aquaculture, particularly those of the genus Asparagopsis. By investigating the
physiological characteristics of the species, the cultivation conditions (sunlight, nitrogen and
carbon) that maximize biofiltration, biomass production and the internal levels of the two major
halogenated metabolites (bromoform and dibromoacetic acid) were established. The species
biofiltration and biomass production performances reported are the highest ever reported for
any other seaweed species in such systems.
8d. Productivity
1. Brun, FG, Olivé, I, Malta, E-j, Vergara, JJ, Hernández, I, Pérez-Lloréns, JL. 2008. Increased
vulnerability of Zostera noltii to stress caused by low light and elevated ammonium levels under
phosphate deficiency. Marine Ecology Progress Series 365: 67-75. IF=2.631.
2. Cabaço, S, Machas, R, Vieira, V, Santos, R. 2008. Impacts of urban wastewater discharge on
seagrass meadows (Zostera noltii). Estuarine, Coastal and Shelf Science 78: 1-13. IF=2.072.
3. Cabaço, S, Santos, R, Duarte, CM. 2008. The impact of sediment burial and erosion on
seagrasses: a review. Estuarine Coastal and Shelf Science 79: 354-366. IF=2.072.
4. Engelen, AH, Espirito-Santo, C, Simões, T, Monteiro, C, Serrão, EA, Pearson, GA, Santos, ROP.
2008. Periodicity of propagule expulsion and settlement in the competing native and invasive
brown seaweeds, Cystoseira humilis and Sargassum muticum (Phaeophyta). European Journal of
Phycology 43: 257-282. IF=1.826.
5. Figueroa, F, Bueno, A, Korbee, N, Santos, R, Mata, L, Schuenhoff, A. 2008. Accumulation of
mycosporine-like amino acids in Asparagopsis armata grown in tanks with fishpond effluents of
gilthead sea bream, Sparus aurata. Journal of the World Aquaculture Society 39: 692-699.
IF=0.693.
6. Leliaert, F, Malta, E-j, Engelen, AH, Mineur, F, De Clerck, O. 2008. Quindao algal bloom culprit
identified. Marine Pollution Bulletin 56: 1516. IF=2.562.
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7. Silva, J, Feijóo, P, Santos, R. 2008. Underwater measurements of carbon dioxide evolution in
marine plant communities: A new method. Estuarine, Coastal and Shelf Science 78: 827-820.
IF=2.072.
proceedings)
proceedings)
MASTER THESES
Cimas, C. 2008. Assessment of the Non-Indigenous Species in Ria Formosa. Erasmus Mundus
European Joint Master thesis in Water and Coastal Management, University of Algarve.
Supervision: A Engelen, A Newton.
Marin, P. 2008. Defining seagrass ecological status in Ria Formosa under the European Water
Framework Directive. Erasmus Mundus European Joint Master thesis in Water and Coastal
Management, University of Algarve. Supervision: S Cabaço, A Newton.
PhD THESES
Mata, L. 2008. Integrated aquaculture of Bonnemaisoniaceae algae: physiological and nutritional
controls of biomass production and of halogenated metabolite content. PhD thesis, University of
Algarve. Supervisor: R Santos.
between Concepts and Measurements. 2008. Coordination of the seagrass research group, Eilat,
Israel.
Evaluation of algal removal impact in the area adjactent to the thermoelectric power plant in
Sines bay. 2008. Research contract, Hidromod/EDP-Electricidade de Portugal.
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Collaborative publication:
-
Brun, FG, et al. 2008. Increased vulnerability of Zostera noltii to stress caused by low
light and elevated ammonium levels under phosphate deficiency. Marine Ecology
Progress Series 365: 67-75.
-
Cabaço, S, et al. 2008. The impact of sediment burial and erosion on seagrasses: a
review. Estuarine Coastal and Shelf Science 79: 354-366.
-
Figueroa, F, et al. 2008. Accumulation of mycosporine-like amino acids in Asparagopsis
armata grown in tanks with fishpond effluents of gilthead sea bream, Sparus aurata.
Journal of the World Aquaculture Society 39: 692-699.
-
Leliaert, F, et al. 2008. Quindao algal bloom culprit identified. Marine Pollution Bulletin
56: 1516.
Collaborative research:
-
Maerlpicon - Estado de conservación de los fondos de maërl en el Atlántico de la
Peninsula Ibérica (2006-2009). Ministerio de Educación y Ciencia, Univ La Coruña, Spain.
-
Envirophyte - Improvement of the cost effectiveness of marine land-based aquaculture
facilities through use of Constructed Wetlands with Salicornia as an environmentally
friendly biofilter and a valuable by-product (2006-2008). FP6-2004-SME-COOP.
-
Volatile organic carbon emissions by marine vegetation (2006-2008). Cooperation
Agreement GRICES/CSIC, IMEDEA, Islas Baleares.
Other international participations:
-
As part of the outcomes of the 8th GAP International Workshop on Gross and Net
Primary Production (2008), where the group coordinated the seagrass research section,
three collaborative publications were produced, which are now in press, to be published
in 2009 (in press):
Silva, J, Sharon, Y, Santos, R, Beer, S. Measuring seagrass photosynthesis: Methods and
applications. Invited review. Aquatic Biology.
Runcie, JW, Paulo, D, Santos, R, Sharon, Y, Beer, S, Silva, J. Photosynthetic responses of
Halophila stipulacea to a light gradient: I – In situ Energy Partitioning of Nonphotochemical Quenching. Aquatic Biology.
Sharon, Y, Silva, J, Santos, R, Runcie, J, Chernihovsky, M, Beer, S. Photosynthetic
responses of Halophila stipulacea to a light gradient: II – Plastic Acclimations Following
Transplantations. Aquatic Biology.
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A team member was appointed to integrate the World Seagrass Association Steering
Committee, as one of the two European representatives in this organ.
Team members integrate Editorial Boards of a number of indexed international scientific
journals.
8e. Future research
1. Objectives
1. Use of marine macrophytes as ecological indicators
Within the Water Framework Directive (WFD) implementation workplan, we will use marine
angiosperms (seagrasses and saltmarshes) and macroalgae as biological elements to classify the
ecological status of the Portuguese coastal and transitional water bodies. Water bodies will be
classified accordingly to the deviations of the Biological Quality Elements (BQE) at reference
conditions. We will also develop new indexes based both on detailed description of species
richness and ecophysiological parameters of key community species.
In the newly approved Eco-Lagunes Interreg-Sudoe project we will work on the development of
new ecophysiological tools and integrative community-based criteria to evaluate the health
status of seagrass dominated ecosystems. The final goal is to improve the environmental
management of lagoon areas guarantying the development of a sustainable economic activity,
while preserving the biodiversity and a normal ecosystem functioning.
The impacts (and their drivers) of invasive seaweeds on seagrass species, communities and
based ecosystems will be investigated from small to large geographical scales (Portugal, US
Pacific and New Zealand), on similar latitudes, to identify general patterns and processes
combining descriptive and experimental approaches.
2. Seagrass productivity and ecosystem management
Our group led a consortium with partners from 9 different countries in the successful
application of a pre-proposal for a COST action. The aim of this Action is the formation of a
network of key research groups and geographic coverage to coordinate research on how
seagrass productivity along European coastlines is affected by alterations in the light
environment and, consequently, how does this affect the ecosystem functioning and services.
The integration of physiological ecologists, molecular and genomic ecologists and resource
management scientists will unravel genotype-phenotype interactions with respect to
productivity and light stress, and thereby develop innovative ecological indicators of seagrass
decline for managers.
217
We will provide an accurate assessment of the carbon budget of a coastal lagoon dominated by
intertidal seagrass and salt marsh meadows (Ria Formosa lagoon), including the organic matter
imports from the watershed and its export to the ocean. Furthermore, we will assess the
metabolic processes that determine the carbon budget, how they respond to the seasonal cycle
and how these are related to the CO2 fluxes with the atmosphere. The impacts of the local
human activity will be assessed simulating different usage scenarios in an
ecological/hydrodynamic model of the system.
We aim to elucidate the fundamental aspects of seagrass photosynthesis in their depth
distribution limits and to evaluate the combined effects of light quality and availability on
photosynthetic performance. We will evaluate the performance of the photosynthetic
apparatus and the biochemical traits of deep-edge plants and determine their responses to
transient changes in PAR spectral composition.
We will investigate the relationships between photosynthesis and calcification in mäerl (red
calcareous algae) species from southern and northern Iberian systems. Specifically, we will
determine in situ the diel and annual patterns of calcification, photosynthesis and community
metabolism in natural mäerl populations and investigate the effects of increased CO2 on the
calcification and photosynthesis of the predominant species in Iberian waters.
2.

Current funding
The accomplishment of the above stated objectives relies on two already approved funding
sources, a major research contract with the Portuguese Water Institute (INAG) and a European
(Interreg Sudoe) project.
100.828 EUR, Interreg Sudoe SOE1/P2/F153, Gestion environnementale des zones lagunaires à
vocation aquacole (ECO-LAGUNES).
229.319 EUR, INAG, Avaliação do Estado Ecológico das Massas de Água Costeiras e de Transição
e do Potencial Ecológico das Massas de Água Fortemente Modificadas (EEMA).

Pending funding
192.542 EUR, FCT PTDC/BIA-BEC/105032/2008, New seagrass index for environmental quality
assessment under the WFD.
199.256 EUR, FCT PTDC/AAC-CLI/103348/2008, Whole-system metabolism and CO2 fluxes in a
coastal lagoon dominated by saltmarsh and seagrass meadows.
199.944 EUR, FCT PTDC/BIA-BEC/101166/2008, Seagrass photosynthesis in changing light
environments: understanding the limits.
199.267 EUR, FCT PTDC/BIA-BEC/101198/2008, Mäerl calcification, photosynthesis and
metabolism in an acidified ocean.
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196.762 EUR, FCT PTDC/MAR/098069/2008, Shifts from seagrass to seaweed dominated
systems - INVASEA.
185.594 EUR, FCT PTDC/MAR/098463/2008, Waterborne seaweed metabolites as a green
solution for antimicrobial therapy in aquaculture.
219
Luísa Maria Pinheiro Valente
LANUCE receive a Pluriannual FCT funding of 21.000 EUR from FCT, which is essentially used to
buy small equipments, and for the laboratory and equipment maintenance. In addition, the
scientific activities of LANUCE are also funded by several active projects, such as:
97.006 EUR, AdI - Programme PRIME, Optidietas - Feed formulation for blackspot seabream and
sole. Project IDEIA 70/00073 in consortium with a feed company, SORGAL, funded Coordinator:
L Valente. Partners: CCMAR, SORGAL.
10.190 EUR, FCT POCTI/MAR/57022/2004, Effects of hydrostatic pressure and other abiotic
factors on growth of the blackspot seabream (Pagellus bogaraveo). Coordinator: J Coimbra. 1
industrial national patent (nr 103 774).
EU FP6-2005-SSP5A, SEACASE - Sustainable extensive and semi-intensive coastal aquaculture in
Sothern Europe. Total funding: 2.391.401 EUR; CIMAR-LA 312.486 EUR, CIIMAR 49.970 EUR.
2007-2009. Partners: LANUCE, IPIMAR and involving researchers of 5 European Countries
(Portugal, Spain, France, Italy and Greece). Coordinator: MT Dinis.
1. Objectives
The central aim of the group is to contribute to the sustainable development of the aquaculture
industry, through basic and applied research directed to the optimisation of diets and cultivation
techniques in order to improve growth and muscle quality. Special attention has been given to
the introduction of new species to aquaculture, namely the blackspot seabream (Pagellus
220
bogaraveo), the sole (Solea senegalensis), the bream (Diplodus sargus) and the octopus
(Octopus vulgaris).
Ongrowing projects deal with the following aspects:
-
Nutritional requirements and optimization of dietary regimes in important species for
aquaculture: Formulation and production of fish diets which promotes maximum
growth performance and better final fish quality; low pollution diets are also
considered. Dietary nutrient metabolism studies involve standard laboratory facilities
for proximate analysis of feed and fish tissues and activity and regulation of key
enzymes involved in lipid metabolism.
-
Growth characterization and flesh quality: Biochemical, histological and histochemical
parameters, namely the tissue fat composition, number and size of muscle fibres and
capacity of recruitment of new fibres are analysed under different rearing conditions.
The laboratory not only uses recent sensory, physical and microbiological analysis to
characterize the “freshness level” of several kinds of seafood, but also is responsible for
the creation of new sensory schemes for cephalopods under the recently developed
Quality Index Method. In the physical methods, this laboratory is using Torrymeter and
Freshmeter instrumental analysis. In the microbiological area, the use of counts of
specific spoilage organisms was developed in practical terms.
-
Olfaction is important in the appetite behavior of fish species, which eventually will
affect feeding and growth performance. Our group will begin in 2009 several research
trials in collaboration with CCMAR to determine olfactory potency of several extracts
and whether the homogenates can be added to commercial aqua feed pellets to
enhance their ingestion.
Nutrient requirements, dietary formulations and feeding practices were optimized for blackspot
seabream, Senegalese sole and bream. Blackspot seabream has a 50% dietary protein
requirement but low lipid tolerance (<10%). Optimal growth of sole is obtained with 8% lipids;
the estimated lysine requirement (4.68 g lysine / 16 g N) was used to design diets using plant
protein sources.
Protein requirement for Diplodus vulgaris was 7.8 g /kg body weight, and this species performed
better than D. sargus. Output: 8 papers (+ 3 submitted); 2 PhD; 1 MSc.
The bottleneck of Octopus culture is the rearing of its paralarval. Microalgal species were used
to improve the biochemical composition of juvenile Artemia as prey for paralarvae. Output: 1
paper published and 1 submitted, 1 PhD.
The production of ornamental aquatic species has now evolved into an industrial activity.
Information regarding nutritional requirements, food utilization and behaviour of ornamental
fish were considered. Output: 3 papers, 2 submitted.
221
Understanding the environmental factors that regulates muscle differentiation and growth is
important to select the best strategy to optimize growth and meat quality a) High-protein diets
favoured muscle hyperplasia; b) High temperature accelerates development and muscle growth
promoting hyperplastic growth. Output: 2 papers, 3 submitted; 1 PhD.
Valorisation of aquaculture products. Production of functional foods. The use of dietary
conjugated linoleic acid (CLA) by fish has a potential benefit for human health. Being fish an
important source of protein and polyunsaturated fatty acids, a further increase in its CLA
content could enhance the nutritional value of fish meat for human consumption. When
supplemented, fish show the highest muscle CLA deposition of any other animal. CLA can be
incorporated up to 1 % in both rainbow trout and sea bass diets contributing to the production
of a functional food.Output: 5 papers, 2 MSc.
Sensory, physical and microbiological characterization of freshness in sea products: New sensory
schemes for cephalopods were developed under the Quality Index Method that was compared
and confirmed using other methods, namely physical methods (Torrymeter and Freshmeter) and
counts of specific spoilage organisms. Output: 4 papers.
8d. Productivity
LANUCE has published in 2008 12 full papers in international journals in the SCI (2.3
papers/researcher). We expect to improve the figures in 2009 due to the large number of
national and international collaborations.
1. Barrento, S, Marques, A, Pedro, S, Vaz-Pires, P, Nunes, ML. 2008. The trade of live crustaceans
in Portugal: space for technological improvements. ICES Journal of Marine Science 65: 551-559.
IF=1.661.
2. Barrento, S, Marques, A, Teixeira, B, Vaz-Pires, P, Nunes, ML. 2008. Essential elements and
contaminants in edible tissues of European and American lobsters. Food Chemistry 111: 862–
867. IF=2.696.
3. Martins da Costa, P, Vaz-Pires, P, Bernardo, F. 2008. Antimicrobial resistance in Escherichia
coli isolated in wastewater and sludge from poultry slaughterhouse wastewater plants. Journal
of Environmental Health 70: 40-45. IF=0.805.
4. Martins da Costa, P., Bica, A, Vaz-Pires, P, Bernardo, F. 2008. Effects of antimicrobial
treatment on selection of resistant Escherichia coli in broiler fecal flora. Microbial Drug
Resistance - Mechanisms, Epidemiology, and Disease 14(4): 266-306. IF=1.800.
5. Mohseni, M, Ozorio, ROA, Pourkazemi, M, Bai, SC. 2008. Effects of dietary L-Carnitine
supplements on growth and body composition in Beluga Sturgeon (Huso huso) juveniles. Journal
of Applied Ichthyology 24: 646–649. IF=0.638.
222
6. Ozório, ROA. 2008. Swimming activity and Non-Protein Energy (NPE) metabolism in fish.
Current Nutrition & Food Science 4: 282-289.
7. Ramos, A, Bandarra, NM, Rema, P, Vaz-Pires, P, Nunes, ML, Andrade, AM, Cordeiro, AR,
Valente, LMP. 2008. Time course deposition of conjugated linoleic acid in market size rainbow
trout (Oncorhynchus mykiss) muscle. Aquaculture 274: 366-374. IF=1.678.
8. Reis, PA, Valente, LMP, Almeida, C, Marisa, R. 2008. A fast and simple methodology for
determination of yttrium as an inert marker in digestibility studies. Food Chemistry 108: 10941098. IF=2.696.
9. Ribeiro, L, Couto, A, Olmedo, M, Alvarez-Blazquez, B, Linares F, Valente, LMP. 2008. Digestive
(Brunnich 1768). Aquaculture Research 39: 339-346. IF=0.991.
10. Seixas, P, Rey-Méndez, M, Valente, LMP, Otero, A. 2008. Producing juvenile Artemia as preys
for Octopus vulgaris paralarvae with different microalgal species of controlled biochemical
composition. Aquaculture 283: 83-91. IF=1.678.
and immunohistochemical study of the fibre types. Tissue and Cell 40: 447-458. IF=0.738.
12. Vaz-Pires, P, Seixas, P, Mota, M, Lapa-Guimarães, J, Pickova, J, Lindo, A, Silva, T. 2008.
Sensory, microbiological, physical and chemical properties of cuttlefish (Sepia officinalis) and
broadtail shortfin squid (Illex coindetii) stored in ice. LWT (Lebensmittel-Wissenschaft und Technologie) - Food Science and Technology 41(9): 1655-1664. IF=2.114.
proceedings)
proceedings)
MASTER THESES
Aguiar Carvalho, CC. 2008. Physiological performance of the Peppery furrow shell Scrobicularia
plana (da Costa 1778) along the European coast. Master in collaboration with the Royal
Netherlands Institute for Sea Research (NIOZ, Netherland), University of Tras-os-Montes e Alto
Douro (UTAD).Supervisor: Henk Van der Veer, Co-supervisor: P Rema.
223
PhD THESES
Barbosa, A. 2008. A study of visual cues that elicit changeable camouflage in cuttlefish. PhD
thesis in Aquatic Sciences, ICBAS, University of Porto. In collaboration with the Marine Biology
Laboratory (MBL, USA). Supervisor: Roger Hanlon, P Vaz-Pires, Gil Rosenthal.
Ribeiro, RA. 2008. Utilização de efluentes industriais (farinha de sangue e soro de leite) na
elaboração de ração para pirarucu (Arapaima gigas, Curvier). PhD thesis in collaboration with
CIIMAR (Portugal), INPA (Brazil) and University of Acre (Brazil). Supervisor: Manuel Pereira
(INPA), R Ozório.
Member of LANUCE have participated in the organization of the most prestige international
conference on Fish Nutrition, as member of organizing committee: 13th International
Symposium on Nutrition and Feeding of Fish, 1-5 June 2008, Florianopolis, Brazil.
LANUCE together with the Aquaculture research group (CCMAR) coordinate the OPTIDIETAS
project in consortium with a feed company SORGAL, S.A. (Project IDEIA 70/00073, Support to
investigation of applied research in companies). This project is focused on the optimization of
dietary formulations and feeding practices for Senegalese sole and blackspot seabream, based
on the specific nutritional requirements and feeding behaviour of each species.
LANUCE together with ICBAS/UP coordinate the ECOPISCIS project in consortium with a
Portuguese aquaculture enterprise Quinta-do-Salmão Ltd. (Project QREN 3442). The aim of
ECOPISCIS is to develop an environmental friendly, long-run alternative to minimize biological
waste from fish production.
LANUCE has long established collaborations with several international Institutes to develop
research programs and co-supervision of PhD students.
-
SEACASE, financed by the EU, involving 5 Countries, coordinated by CCMAR.
-
NIFES, Bergen, Norway. Co-supervision of 1 PhD thesis (Marit Espe): 1 publication.
-
UMR, Nutrition Aquaculture Génomique, INRA, St-Pee-Sur Nivelle, France. Cosupervision of a PhD thesis by Geneviéve Corraze: 1 publication. 1 COST action 825.
224
-
Bødo University, Norway. Co-supervision of a PhD thesis by Jorge Fernandes. 1 COST
action 825, 1 proposal for a PhD thesis.
-
NIOZ; Royal Netherlands Institute for Sea Research, The Netherlands. Co-supervision of
1 MSc thesis (CC de Aguiar Carvalho).
-
Barcelona University, Spain (Joaquin Gutierrez). 1 publication. 1 COST action 825.
-
Instituto Español de Oceanografia and Consellería de Pesca e Asuntos Marítimos
(CPAM), Spain (Peleteiro, T, Olmedo, M, Linares, F, Villanueva, JLR). 1 Interreg project
submitted and 1 Spanish project. 1 publication (Valente, LMP, Olmedo, M, ÁlvarezBlázquez, B, Borges, P, Soares, S, Gomes, EFS, Pazos, G, Linares, F. 2008) .
-
University of Santiago de Compostela (USC), Spain. Co-supervision of 1 PhD thesis
(Manuel Rey Mèndez and Ana Otero). 1 publication.
-
INPA, National Institute for Research in the Amazon, Brazil (Manoel Pereira); UFAC and
Universidade Federal do Acre (Ricardo Amaral), Brazil. 1 PhD thesis.
-
Iran’s International Sturgeon Research Institute, Rasht, Iran. 1 publication (Mohseni, M,
Ozorio, ROA, Pourkazemi, M, Bai, SC. 2008. Journal of Applied Ichthyology 24:646-649).
LANUCE has participated in several meetings promoted by the Comissão de Coordenação para o
Desenvolvimento da Região Norte (CCDR-N), contributing to the elaboration of regional
priorities for the sustainable development of marine research in the North of Portugal and
aliza region that resulted in a final report “Regional Agenda for the Sea”.
8e. Future research
1. Objectives
In the Division of Aquaculture & Marine Biotechnology of CIMAR-LA, there are several groups
specifically dedicated to the research line Aquaculture. The objective of LANUCE is to contribute
to the implementation and consolidation of Fish Nutrition through a more thigh collaboration
with those groups. Until 2008, 5 collaborative projects have already been established,
generating funds of 1.13 Million EUR. LANUCE and AQUAGROUP have currently joint PhD
projects with the participation of international groups. These co-supervised projects were
elaborated in order to optimize the expertise of each group and to guaranty the student the
best institutional facilities to develop the proposed research line in the due time.
In specific terms, the central aim of LANUCE still is contributing to the sustainable development
of the aquaculture industry. Plant protein ingredients will be considered for dietary
incorporation.
225
The effects of such diets on fish growth and on the final muscle quality will be evaluated. The
optimization of feeding regimes and rearing conditions of Senegalese sole will be a priority in
collaboration with Aquagroup from CCMAR. Large-scale experiments will be considered to
validate our laboratory-scale results. These are applied research lines that we expect to develop
further in collaboration with fish farmers from Portugal and North Spain.
Particular attention will be given to the development of new tools to assess muscle growth and
final quality to combine with the classic morphometric approach already implemented in our
laboratory. Understanding the molecular basis of environmental-induced phenotypic plasticity
of muscle development and growth will be a main research line.
LANUCE will reinforce its activity towards the improvement of food quality and security of
fisheries and aquaculture products. The predicted work includes the further development of
sensory schemes based on Quality Index Method, the most recent sensory classification system
for seafood items.
In terms of physical methods, new methods for the evaluation of rigor mortis will be developed,
as well as others that proved to be efficient and useful in quality and welfare evaluations, in
addition to the already used instrumental methods (Torrymeter and Freshmeter). In terms of
microbiology, the first studies will be focused on the characterization of the microbiological
environment of freshwater and seawater farms, including animals, water and surfaces, and
eventual resistances to antibiotics (antibioresistances) shown by commensal (Escherichia coli
and Enterococcus spp.) and pathogenic bacteria (Salmonella sp.); their ability to transmit those
resistances to humans directly from the consumption of farmed products and also by the
aquatic environment will also be considered.
Cage aquaculture is a new technology promising high, but large amounts of feed is directly or
indirectly released to the surrounding environment as excreted wastes. In the LANUCE
researchers’ viewpoint, the solution to minimize the environmental impact of the cage
aquaculture can be achieved by improving the aquaculture technology, more particularly by
developing automated and eco-friendly cage aquaculture technology. Thus, LANUCE is carrying
out studies to understand how fish control their feed intake, and to develop tools to increase
waste recovery. In that way, LANUCE expertise may help the aquaculture sector designing feed
and feeding strategies to optimize production, minimizing feed waste.
2.

Current funding
SEACASE, EU FP6-2005-SSP5A. Total funding: 2.391.401 EUR; CIMAR-LA: 312.486 EUR, CIIMAR:
49.970 EUR. 2007-2009. Coordinator: MT Dinis.
FCT PTDC/MAR/70858/2006, TEXBREAM - Post-mortem changes in gilthead sea bream muscle
proteins: its implications to flesh texture. Total funding: 188.327 EUR 2008-2011. Coordinator: J
Dias (CCMAR).
226
ECOPISCIS - Waste Management in Fish Production. Project QREN 3442. Total funding: 591.369
EUR, CIIMAR 40.200 EUR. 2009-2011. Coordinators: JF Gonçalves (ICBAS), R Ozório.

Pending funding
217.153 EUR, DRP-Acções Colectivas (PROMAR/1198/2006), PROALGA. Coordinator: P Rema.
40.000 EUR, QREN, PROAMBIENTE. Coordinator: L Valente.
92.000 EUR, EDP funding for Biodiversity, Conservation of trout populations in the rivers of
North Portugal: importance of the genetic preservation and ecological assessment. Coordinator:
P Rema.
154.383 EUR, FCT PTDC/MAR/099948/2008, PROBIOFISH. Coordinator: R Ozório.
145.334 EUR, FCT PTDC/MAR/099952/2008, MODELFISH. Coordinator: R Ozório.
127.873,20 EUR, FCT PTDC/MAR/099996/2008, SOLIPIDS. Coordinator: L Valente.
99.312 EUR, FCT PTDC/MAR/098496/2008, EPISOLE. Coordinator: L Valente.
89.304 EUR, FCT PTDC/BIA-BEC/105266/2008, Coordinator: A Correia (LECOF).
107.000 EUR, FCT PTDC/MAR/098038/2008, Stress in estuarine glass eel. Coordinator: J Wilson
(LECOF).
199.998 EUR, FCT PTDC/MAR/105229/2008, BENEFITS. Coordinator: I Sousa Pinto (Biodiversity
of Aquatic Ecosystems Group).
199.488 EUR, FCT PTDC/MAR/100209/2008, Diet optimization for zebra sea bream juveniles, a
new species for aquaculture: meeting nutritional requirements. Coordinator: A Oliva-Teles (Lab.
of Fish Nutrition).
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Maria Luisa Machado Cerqueira Bastos
-
-
National funding
3.665,95 EUR, FCT POCTI/CTA/49563/2002, NICC, 02/2005-07/2008.
13.134,26 EUR, APDL, River Douro Estuarine Contributions to Inner Shelf Dynamics: Data
collection in Douro and Minho for Estuarine Characterization.
40 000 EUR, FCT PPCDT/MAR/57265/2004. HI: Univ. Madeira, PI: Rui Caldeira (currently at
CIIMAR). 01/2006-12/2009.
-
European funding
3.000 EUR. HYDRALAB-III European funding initiative by FP6 Island wake studies. Grenoble
Coriolis platform, 3-28 Nov 2008.
Travel and meeting costs, Co-I, NERC NE/C003438/1, Global Loading and Deformation at Tidal
Timescales, 2006-2009.
Travel and meeting costs, COST Action ES0701, supported by the EU RTD, trips to attend the 2nd
management committee meetings on 27-28 Oct 2008, UK.
228
1. Objectives
The Coastal and Ocean Dynamics group is a multidisciplinary group undertaking research in
areas such as:
-
The analysis and modelling of physical and biological oceanographic processes towards a
better understanding of the circulation patterns in NE Atlantic, using in-situ, modelled
and remote sensing data;
-
Determination of sea surface topography as a contribution to model surface currents;
-
Studies of the ocean (sub)mesoscale dynamics.
Support to environmental management and impact evaluation studies through the exploitation
of GNSS based techniques for the morphodynamic characterization of sandy littorals and
assessment of sediments transfer; exploitation of laser systems integrated with GNSS/INS
sensors for surveys towards coastal monitoring and detection of pollutants.
The participation in the RAIA project, a new Portuguese-Spanish ocean observatory, approved in
2008, was part of the group strategy to contribute for the implementation of a regional ocean
forecasting system for the North Iberian Peninsula through the exploitation of in-situ data for
oceanographic modelling with data assimilation.
This project strengthens ongoing collaboration links with local authorities and private
companies. An example is the recent installation of a GPS station in the harbour of Leixões,
Porto, to monitor the tide gauge stability in the coming years and a project to analyse its 100
years long tide gauge record was initiated. This was a joint venture with APDL to contribute to a
better understanding of long term sea-level changes along the Portuguese coast.
The group intends to further engage in the training of young scientists as well as post-doc
researchers and to promote the development and exploitation of new marine technologies, and
observational methodologies to support studies of ocean coastal dynamics in cooperation with
other universities, research groups and companies. The work is strongly interdisciplinary,
involving cooperation within the CIMAR as well as with researchers from other national and
international Centres.
2008 was a year of change and expansion for CIIMAR. the former Marine Technology group,
broadened its research areas and capabilities within the Associated Lab, making way for the
Coastal and Ocean Dynamics group and including oceanography studies such as, modelling of
physical and biological oceanographic processes and coastal and estuarine studies.
Main achievements were:
-
Acquisition and setup of a High Performance Computer,a key aspect towards the
development of oceanographic numerical modelling.
229
-
Approval of the Interreg IV funded project RAIA, Observatorio oceánico del margen
ibérico, which will allow for the implementation of an ocean observation network for
the consolidation of operational oceanography on the Spanish-Portuguese Iberian coast.
-
Approval of a project submitted to APDL to analyse its 100 years long tide gauge record.
The results will contribute to a better understanding of long term sea-level changes
along the Portuguese coast.
-
Use of Mobile Mapping Technologies to build Digital Terrain Models (DTM) for coastal
erosion studies, in particular the characterization of the morphodynamic evolution of
sandy littorals.
-
Contributions to the COST Action “Improved Constraints on Models of lacial Isostatic
Adjustments”.
-
Collaboration in the development of new Lagragian drifters based on GPS/GSM
technology.
-
Successfully conclusion of pending projects (NICC, ECOIS and TIPGRav)
-
Participation in international projects that are studying breakthrough aspects of island
wake dynamics.
-
Active role on building an international network of specialists interested in the study of
island wakes and ocean (sub) mesoscale dynamics.
-
Preparation of an ESA Category-1 project “Fate of island induced (sub) mesoscale
oceanic features in the NE Atlantic”.
8d. Productivity
1. Araújo, IB, Pugh, DT. 2008. Sea Levels at Newlyn 1915-2005: Analysis of trends for future
flooding risks. Journal of Coastal Research: 24(sp3): 203-212, doi: 10.2112/06-0785.1. IF=0.517.
2. Catalao, J, Bos, MS. 2008. Sensitivity analysis of the gravity geoid estimation: A case study on
the Azores plateau. Physics of the Earth and Planetary Interiors 168(1-2): 113-124. IF=2.353.
3. Bos, MS, Fernandes, RMS, Williams, SDP, Bastos, L. 2008. Fast error analysis of continuous
GPS observations. Journal of Geodesy 82(3): 157-166. IF=1.689.
4. Penna, N, Bos, M, Baker, T, Scherneck, H-G. 2008. Assessing the accuracy of predicted ocean
tide loading displacement values. Journal of Geodesy 82(12): 893-907. IF=1.689.
230
5. Cruz, C, Bio, AMF, Julliotti, A, Tavares, A, Martins-Loução, MA. 2008, Heterogeneity of soil
surface ammonium concentration and other characteristics, related to plant specific variability
in a Mediterranean-type ecosystem. Environmental Pollution 154: 414–423. IF=3.135.
6. Thomas, PJ, Murray, AS, Granja, HM, Jain, M. 2008. Optical dating of Late Quaternary coastal
deposits in Northwestern Portugal. Journal of Coastal Research 24(2B): 134-144. IF=0.517.
7. Granja, HM, De Groot, Th, Costa, AL. 2008. Evidence for Pleistocene wet aeolian dune and
interdune accumulation, S. Pedro de Maceda, NW Portugal. Sedimentology 55(5): 1203-1226.
IF=1.937.
proceedings)
proceedings)
1. Baptista, P, Bastos, L, Cunha, T, Bernardes, C, Dias, J. 2008. Aplicação de Metodologias de
Monitorização GPS em Litorais Arenosos: Geração de Modelos de Elevação do Terreno. Revista
de Gestão Costeira Integrada - Revista n.º 8, fascículo 1 - Ano 7.
2. Silva, AJ, Martins, IM, Santos, AI, Moita, MT, Bastos, LC. 2008. Is there a Northwest Iberian
Coastal Current? 1º Seminário sobre Investigação na Zona Costeira da rede PoCoast, 26-28 de
Maio de 2008, Porto, Portugal.
MASTER THESES
Rodrigues, J. 2008. A avifauna do estuário do Rio Minho (aves aquáticas). Caracterização,
importância e perspectivas de uso turístico. Master thesis in Sciences of the Sea – Sea
Resources, ICBAS, University of Porto. Supervisor: António Manuel da Silva Luís (University of
Aveiro), Co-Supervisor: AMF Bio.
Organization of International Workshop to discuss dynamics of island wakes. 21-31 July 2008.
This workshop was co-sponsored by CIIMAR, FCT, IST-MARETEC and CCM-UMa, and included the
participation of researchers from Portugal, France, Spain, Italy and USA. Several aspects of
Geophysical Fluids Dynamics and instabilities were discussed. The November 2008, HYDRALABIII laboratory study plan were also discussed. (http://wakes.uma.pt/workshop/). The event lead
to the proposed layout of a review paper on the deep-water island wakes.
231
Contract research between Port Authority of Douro and Leixões - APDL and CIMAR for the
following projects:
-
River Douro Estuarine Contributions to Inner Shelf Dynamics: Data collection in Douro
and Minho for Estuarine Characterization, 01/05/2005-31/07/2008, 77.000 EUR.
-
Conservação e análise do registo histórico dos marégrafos de Leixões, 01/2009-12/2010,
65.000 EUR.
Participation in the second Management Committee Meeting of the COST Action ES0701 in
Newcastle, UK, 27 October 2008.
Cooperation with the Onsala Space Observatory, Sweden, to improve the internet ocean tide
loading provider.
Solicitation to peer-review manuscript from the following journals:
-
Continental Shelf Research
-
Journal of Hydrology
-
Journal of Geodynamics
-
Journal of Coastal Research
-
Remote Sensing of the Environment
8e. Future research
1. Objectives
The RAIA project, a new Portuguese-Spanish ocean observatory, will be one of the group main
integrating activities. Within this project several activities of the COD group are foreseen:
-
232
Combination of in-situ data with remotely sensed data to study ocean mesoscale
dynamics; improvement of coastal ocean models using the derived boundary conditions;
assimilation of sea-surface height dynamics (estimated from improved geoid and
satellite altimetry data) into ocean models; use of the remote sensing data such as SAR
to complement in-situ and modelling data.
-
The overall purpose is to contribute to build an improved ocean forecasting tool for the
NE Iberian Peninsula. The coastal ocean dynamics will be modelled using a Regional
Ocean Model System (ROMS).
-
Modelling of ocean tides: development of global and regional models which can be used
to improve our knowledge of the tides in f Portugal and around Madeira. Assimilation of
tide gauge data to improve these models is also foreseen.
-
Analysis of historical and actual tide gauge data towards the evaluation of sea-level
variations along the Portuguese coast, including reliability assessment.
-
Exploitation of SAR observations to contribute to the study of island induced oceanic
features and their fate in the NE Atlantic Circulation.
-
Promote the development and exploitation of new marine observational technologies
(terrestrial, marine and aerial), and methodologies to support studies of ocean and
coastal dynamics (e.g. mobile mapping systems for coastal monitoring; shallow water
bathymetry; airborne gravimetry for regional geoid determination).
-
Publication, of already achieved results from current research projects, in peer-reviewed
journals.
-
Conclude the installation of CIIMAR High Performance Computer in collaboration with
University of Porto GRID-team, including its insertion on the international ‘E EE Enabling Grids for E-sciencE ‘.
-
Organize the II edition of an international workshop on “dynamics and instabilities of
Island wake regimes”.
-
Involvement in international and national Master and PhD programs in Marine Science
as well as training of young scientists, at the PhD and Post-doc level.
-
Strengthen the cooperation with other national and international research Centres and
as well as with public institutions and private companies.
2.

Current funding
43.800 EUR, FCT ERA-NET AMPERA, DEOSOM - Detection and Evaluation of Oil Spills by Optical
Methods, 09/2008-08/2011.
121.651 EUR. INTERREG IV POCTEP, RAIA - Observatorio Oceánico del Margen Ibérico, 01/2009–
12/2011 (Approved September 2008).
65.000 EUR, Funding: APDL, Conservação e análise do registo histórico dos marégrafos de
Leixões, 01/2009-12/2010.
233
Travel and meeting costs, COST Action ES0701 – This action addresses the current uncertainty in
polar ice mass contributions to present-day global sea level rise. COST is supported by the EU
RTD Framework Programme, 2008-2010.
Travel and meeting costs, Co-I, NERC NE/C003438/1, Global Loading and Deformation at Tidal
Timescales, 2006-2009.

Pending funding
23.232 EUR, FCT PTDC/MAR/102804/2008, MISYCOAST - Mobile Imaging SYstem for Coastal
dynamics, 2010-2013.
175.000 EUR, FCT PTDC/AAC-CLI/099631/2008, CFLUX - Continental shelf pump hypothesis,
2010-2013.
FCT PTDC/MAR/101429/2008, Island-eddy interactions: Madeira Archipelago case study.
234
Pathology
Pathology
Jorge Guimaraes da Costa Eiras
The funding for the research performed within the period was provided mainly by pluriannual
FCT funds (17.000 EUR). Funding was also provided by the Treaty of Windsor Anglo-Portuguese
Joint Research Programme (750 EUR).
1. Objectives
The objectives of the group continue to be:
-
Contribute to the knowledge of fish parasites specially that ones with greater impact in
fisheries, aquaculture and public health.
-
Use of parasites as biological tags for marine and estuarine fish population’s with
emphasis on the discrimination of commercially important Portuguese, Brazilian and
Angolan fishery species.
-
Contribute to the knowledge of Digenea parasite species that infect several hosts
collected in different shallow-water ecosystems; perform a morpho-anatomic and
molecular characterization of the different parasitic stages allowing their identification;
contribute to the ecoparasitological characterization and clarification of digenean life
cycles; develop a database of Digenea ITS1 sequences.
-
Contribute to the knowledge of bacterial fish diseases.
-
Perform studies on the detection and isolation of bacteriophages from the
environment and their use as prophylactic and therapeutic agents against fish
235
pathogenic bacteria.
Results obtained allowed the publication of several papers (14, plus 7 in press and 6 submitted)
in international referred journals, the presentation of a number of communications in
international (5) and national (3) meetings, as well as the edition of a textbook on fish diseases
[Eiras, JC, Segner, H, Wahli, T, Kapoor, B. (eds). 2008. Fish Diseases. Science Publishers, Inc.,
Enfield, NH, USA. 2 Vol., 1308 pp].
During this period 2 PhD and 3 MSc students performed their research in the Laboratory and 1
PhD and 3 MSc theses were completed with success.
The use of parasites for discriminating fish stocks of black scabbard fish Aphanopus carbo from
Portuguese waters was evaluated. The characterization of Anisakis spp. larvae (a parasite which
constitutes a potential risk for human health) infection in A. carbo was also done. Several papers
on the subject were submitted for publication.
The study of metazoan parasites of Cyprinus carpio from Mozambique, one of the most
important freshwater fish species farmed in this developing country was achieved.
8d. Productivity
1. Azevedo, C, Casal, G, Matos, P, Matos, E. 2008. A new species of Myxozoa, Henneguya
rondoni n. sp. (Myxozoa), from the peripheral nervous system of the Amazonian fish,
Gymnorhamphichthys rondoni (Teleostei). Journal of Eukaryotic Microbiology 55: 229-234.
IF=1.502.
2. Azevedo, C, Casal, G, Montes, J. 2008. Ultrastructural developmental cycle of Haplosporidium
montforti (Phylum Haplosporidia) in its farmed abalone host, Haliotis tuberculata (Gastropoda).
Journal of Parasitology 94: 137-142. IF=1.165.
3. Boane, C, Cruz, C, Saraiva, A. 2008. Metazoan parasites of Cyprinus carpio L. (Cyprinidae) from
Mozambique. Aquaculture 284: 59-61. IF=1.678.
4. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2008. A new microsporidian parasite,
Potaspora morhaphis n. gen., n. sp. (Microsporidia) infecting the teleostean fish Potamorhaphis
guianensis from the river Amazon. Morphological, ultrastructural and molecular
characterization. Parasitology 135: 1053-1064. IF=2.071.
5. Casal, G, Matos, E, Matos, P, Azevedo, C. 2008. Ultrastructural description of a new
myxosporean parasite Kudoa aequidens sp. n. (Myxozoa, Myxosporea), found in the subopercular musculature of Aequidens plagiozonatus (Teleostei) from the Amazon river. Acta
Protozoologica 47: 135-141. IF=1.013.
236
6. Davies, AJ, Amado, LL, Cook, RT, Bianchini, A, Eiras, JC. 2008. Potential environmental and
host ender influences on prevalence of Haemogregarina platessae (Adeleorina:
Haemogregarinidae) and suspected Haemohormidium terranovae (incerta sedis) in Brazilian
flounder from the Patos Lagoon Estuary, Southern Brazil. Folia Parasitologica 55: 161-170.
IF=1.307.
7. Diniz, DA, Varella, JEA, Guimarães, MDF, Santos, AFL, Fujimoto, RY, Monfort, KCF, Pires, MAB,
Martins, ML, Eiras, JC. 2008. A note on the occurrence of praniza larvae of Gnathia sp.
(Crustacea, Isopoda, Gnathiidae) on fishes from Northeast of Pará, Brazil. Anais da Academia
Brasileira de Ciências 80(4): 657-664. IF=0.881.
8. Eiras, JC, Chemes, SB, Casabianca, OA, Takemoto, R, Rossi, LM, Pavanelli, GC. 2008. An
anusual infection of Henneguya sp. (Myxozoa, Myxosporea, Myxobolidae) in the gills of
Hoplosternum littorale Hancock, 1928 (Osteichtyhes, Callichthyidae) from Middle Paraná River,
Santa Fe, Argentina. Bulletin of the European Association of Fish Pathologists 28: 22-26.
IF=0.430.
9. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2008. Henneguya caudicula n. sp. (Myxozoa,
Myxobolidae), a parasite of Leporinus lacustris (Osteichthyes, Anostomidae) from the high
Paraná River, Brazil, with a revision of Henneguya spp. infecting South American fish. Acta
10. Martins, RF, Ramos, MF, Herfindal, L, Sousa, JA, Skaerven, K, Vasconcelos, V. 2008.
Antimicrobial and cytotoxic assessment of marine cyanobacteria - Synechocystis and
Synechococcus. Marine Drugs 6(1): 1-11. IF=1.200.
11. Hermida, M, Saraiva, A, Cruz, C. 2008. Metazoan parasite community of a european eel
(Anguilla anguilla) population from an estuary in Portugal. Bulletin of the European Association
of Fish Pathologists 28(1): 35-40. IF=0.430.
12. Saraiva, A, Hermida, M, Costa, MJ, Maia, C, Reis, AR, Cruz, C, Valente, A. 2008. First record of
Philometra ovata (Nematoda) infection on Gobio lozanoi in Portugal. Journal of Fish Biology 73:
2288-2292. IF=1.246.
13. Scholz, T., Boane, C, Saraiva, A. 2008. New Metacestodes of gryporhynchid tapeworms
(Cestoda: Cyclophyllidea) from carp (Cyprinus carpio Linnaeus, 1758) from Mozambique, Africa.
Comparative Parasitology 75(2): 315-320. IF=0.477.
14. Costa, G, Santos, MJ, Costa, L, Biscoito, M, Pinheiro de Carvalho, MA, Melo-Moreira, E. 2008.
Helminth parasites from the stomach of conger eel, Conger conger from Madeira Island, Atlantic
Ocean. Journal of Parasitology, doi:10.1645/GE-1760.1. IF=1.165.
proceedings)
1. Eiras, JC, Segner, H, Wahli, T, Kapoor, B. (eds). 2008. Fish Diseases. Science Publishers, Inc.,
Enfield, NH, USA. 2 Vol., 1308 pp.
237
2. Afonso-Dias, I, Menezes, G, MacKenzie, K, Eiras, JC (eds.). 2008. Proceedings of the
International Workshop on Marine Parasitology: Applied Aspects of Marine Parasitology.
Arquipélago. Life and Marine Sciences, Supplement 6, xiv + 49 pp.
proceedings)
MASTER THESES
Duarte, D. 2008. Parasitismo em Talorchestia brito (Stebbing, 1891) na Região Norte e Centro da
Costa Portuguesa. Master thesis, Faculty of Sciences, University of Porto. Supervisor: MJ Santos.
Rangel, LF. 2008. Parasitas metazoários de poliquetas (Nereis diversicolor e Diopatra
neapolitana) da Ria de Aveiro. Master thesis in Applied Ecology, Faculty of Sciences, University
of Porto. Supervisor: MJ. Santos.
Severino, R 2008. Contributo para o conhecimento do ciclo de vida de Amyloodinium ocellatum
e seu controlo em Piscicultura. Master thesis, Faculty of Sciences, University of Porto.
Supervisor: MJ Santos.
PhD THESES
Boane, C. 2008. Parasitas de carpa de Moçambique. PhD thesis, Faculty of Sciences, University
of Porto. Supervisor: A Saraiva.
Member of Scientific committee of the International Meeting “New approches about
Neotropical Helminthology” Organized by the Peruvian Helminthology and Associeted
Invertebrates Assotiation, 30 October-1 November 2008, Lima, Peru. (A. Saraiva).
Collaboration with institutions from different countries:
-
238
Univ. Kingston, UK (Prof. Angela Davis);
-
Natural History Museum, London, UK (Prof. David Gibson);
-
Univ. Maringá, Brazil (Prof. Gilberto Pavanelli, Prof. Ricardo Takemoto);
-
Univ. Rio Grande, Brazil;
-
Univ. Londrina, Brazil (Prof. Ângela Silva-Souza);
-
Fisheries Institute of São Paulo, Brazil (Prof. Maria José Ranzani);
-
Hungarian Academy of Science, Hungary (Prof. K Molnár; Prof. C Szekély);
-
Czech Academy of Sciences, Czech Republic (Prof. F Moravec, Prof. T Scholz);
-
California State Univ. Long Beach, USA (Prof. Jo-shey Ho);
-
Oregon State Univ, Department of Zoology, Corvallis, USA (Prof. George Poinar);
-
Carlos Azevedo Research Laboratory, Federal Rural Univ. Amazónia, Belém; Brazil (Prof.
Edilson Matos);
-
Federal Univ. Pará, Belém, Brazil (Prof. Patrícia Matos);
-
Instituto de Investigaciones Marinas,Vigo, Spain (Dr. António Figueras, Dr. Camiño
Gesta);
-
Centro de Control do Medio Mariño (CCMM), Consellería de Pesca e Asuntos Marítimos,
Spain (Dr. Jaime Montes);
-
Humboldt State Univ., California, USA (Dr. Katie R McGourty);
-
Federal Univ. Pernambuco, Recife, Brazil (Prof. Paulo Padovan, Prof. Isaíras Padovan);
-
Federal Univ. Piauí, Fac. Med. Veterinária, Teresina, Brazil (Prof. Ivete Mendonça);
-
Federal Univ. Santa Catarina, Florianópolis, Brazil (Prof. Patrícia Garcia);
-
Universidade Federal Fluminense, Fac. Med. Veterinária, Niterói, Brazil (Prof. Sérgio
Carmona, Prof. Marcelo Knoff);
-
Universidade Federal Rural do Rio de Janeiro (Prof. José Luis Luque);
-
Enbrapa, Corumbá, Mato Grosso do Sul, Brazil (Dr. Débora Marques);
-
Instituto Nacional de Investigação Pesqueira de Luanda, Angola (Ana Paula, Nelson
Samuel).
Evaluation of projects was conducted to the Czech Science Foundation (GA CR) (A Saraiva).
239
Referees of several peer review Journals (Veterinary Parasitology, Diseases of Aquatic
Organisms, Journal of Parasitology, Acta Protozoologica, etc.).
Evaluation of PhD thesis “Feeding habits and Nematode parasites of some fishes of Karachi
coast” of the Jinnah Univ. for Women, Nazimabad, Karachi, Pakistan (MJ Santos).
Members of Scientific committees of the International Meeting “New approches about
Neotropical Helminthology” Organized by the Peruvian Helminthology and Associeted
Invertebrates Assotiation, 30 Oct.- 1 Nov. 2008, Lima, Peru. (A Saraiva).
Portuguese contact of the International Ichthyoparasitology Newsletter (MJ Santos).
8e. Future research
1. Objectives
The objective for the next few years is to continue the research on fish parasitology and fish
bacteriology.
It is intended to continue the study of the systematic of Myxozoan fish parasites, as well as the
host-parasite relationship. The identification of new species shall involve traditional and
molecular methods. On the other hand, we intend to intensify the study of the alternate stages
of the life cycle of Myxozoans, the actinospore stages infecting oligochaetes and polichaetes,
once this subject is practically unknown in Portugal. It is also intended to perform experimental
laboratory studies on the life cycle of these parasites.
The study parasites of the Phyla Microsporidia, Apicomplexa and Haplosporidia in freshwater
and marine animals, such as fishes, crustaceans and molluscs from Ibero-Atlantic and Brazilian
fauna will continue. Morphological and ultrastructural characterization of the parasites by light
microscopy, scanning (SEM) and transmission electron microscopy (TEM). Studies of the life
cycle, pathology associated to the parasites and studies of cytochemistry. Molecular studies to
identify the parasites by phylogenetic characterization of the conserved genes.
Concerning the the studies of Digeneans it is intended to continue the search and identification
of Digenea parasites from hosts collected in several shallow-water ecosystems and perform the
characterization of their larval stages morpho-anatomically, by light and scanning electron
microscopy, and with the use of molecular data; clarify more digenean life cycles; study the
effects that parasites exert on their hosts and its influence in the ecosystems dynamics; study
spatial heterogeneity (based on molecular methods) between sites (local parasite faunas) and
within sites (individual parasite faunas); increase the digenean species molecular database and
perform phylogenetic studies.
240
Studies on metazoan parasites communities will be performed on some commercial fish in order
to evaluate i) the use of parasites as biological tags and ii) their effects in fish populations and
fisheries
Evaluation of some parasite infection which may cause negative effects (reducing the market
value of fish, being a potential risk for human health or decreasing fish stocks) will be made in
fish species used for human consumption.
Studies on the detection and isolation of bacteriophages from the environment and their use as
prophylactic and therapeutic agents against fish pathogenic bacteria will continue.
2.

Current funding
750 EUR; CRUP, B-58/07 (B-13/05), 2007-2008.

Pending funding
78.000 EUR; FCT PTDC/BIA-BEC/098509/2008, 2009-2012.
241
Synthesis and Organic Reactivity
1. Group nName / denomination
Group of Synthesis and Organic Reactivity
Maria Lurdes Santos Cristiano
94.864,00 EUR, FP7 Cooperation Work Programme: Theme 1 – Health. Health-2007-2.3.2-4:
Addressing Knowledge Gaps in Pregnancy Malaria – ARTEMIP - The safety pharmacology of
artemisinins when used to reverse the pathophysiology of malaria in pregnancy. Starting date:
March 2008.
20.400,00 EUR, FCT/PTDC/QUI/67674/2006, Studies on the reactivity of derivatives of tetrazole
and isothiazole; isothiazolyl-tetrazoles as potentialbidentate ligands and their application in
catalysis.
1. Objectives
The objectives for 2008 were to continue research in the area of Physical Organic Chemistry,
directed to the investigation of structure and reactivity of bioactive compounds. A major
investment was made in the design and synthesis of endoperoxide-based antimalarials and
falcipain inhibitors. The targets are designed within the concept of combination chemotherapy,
whereby two active pharmacophores, directed to different intracellular targets, are combined
within the same chemical entity. A major effort has been made towards meeting the objectives
of the ARTEMIP project, started in March 2008.
During 2008 the group has boosted research in the structure and reactivity of bioactive
compounds derived from saccharin and tetrazole. Part of the work was published in peerreviewed journals and presented in international meetings, namely the IUPAC International
242
Conference on Physical Organic Chemistry. The PI of the group, MLS Cristiano, became a
member of the IUPAC Subcommittee on Structural and Mechanistic Chemistry, and is, since
2007, a member of the European Advisory Board on Organic Reactivity. More work is in
progress, and project proposals were submitted. The access to equipment required, not
available at University of Algarve, is granted by our collaborator Rui Fausto, at the University of
Coimbra.
In the area of Medicinal Chemistry, new target compounds were prepared and sent to biological
screening. The work in this field is extremely important but relies strongly on collaborations
from abroad (Liverpool, Leiden, Gotenborg, WhO/TDR), due to the lack of MNR and adequate
mass spectrometry facilities in the Algarve. The main efforts were put into the ARTEMIP project.
Goals for the first year were met, and the project is developing as initially expected. A project
proposal was submitted to FCT on the design of multi-target antimalarial drugs.
In conclusion, during 2008 the group has made considerable progress in both areas, despites
difficulties related to unavailability of fundamental equipment.
8d. Productivity
1. Almeida, R, Gomez-Zavaglia, A, Kaczor, A, Cristiano, MLS, Eusebio, MES, Maria, TMR, Fausto,
R. 2008. First observation of Chapman rearrangement of a pseudosaccharyl ether in the solid
state: the thermal isomerization of 3-(methoxy)1,2-benzisothiazole 1,1-dioxide revisited.
Tetrahedron 64: 3296-3305. IF=2.897.
2. Frija, LMT, Khmelinskii, IV, Serpa, C, Reva, ID, Fausto, R, Cristiano, MLS. 2008. Photochemistry
of 5-allyloxy-tetrazoles: steady-state and laser flash photolysis study. Organic & Biomolecular
Chemistry 6: 1046-1055. IF=3.550.
3. Kaczor, A, Almeida, R, Gomez-Zavaglia, A, Cristiano, MDS, Fausto, R. 2008. Molecular
structure and infrared spectra of the monomeric 3-(methoxy)-1,2-benzisothiazole 1,1-dioxide
(methyl pseudosaccharyl ether). Journal of Molecular Structure 876: 77-85. IF=1.594.
4. Kaczor, A, Proniewicz, LM, Almeida, R, Gomez-Zavaglia, A, Cristiano, MLS, Beja, AMM, Silva,
MR, Fausto, R. 2008. The Chapman-type rearrangement in pseudosaccharins: The case of 3(methoxy)-1,2-benzisothiazole 1,1-dioxide. Journal of Molecular Structure 892: 343-352.
IF=1.594.
5. Veríssimo, E, Berry, N, Gibbons, P, Cristiano, MLS, Rosenthal, PJ, Gut, J, Ward, SA, O'Neill, PM.
2008. Design and synthesis of novel 2-pyridone peptidomimetic falcipain 2/3 inhibitors.
Bioorganic & Medicinal Chemistry Letters 18: 4210-4214. IF=2.531.
6. Cristiano, MLS, Ruasse, M-F. 2008. Editorial. Journal of Physical Organic Chemistry 21: 223.
IF=1.415.
243
proceedings)
1. Gomez-Zavaglia, A, Reva, ID, Frija, LMT, Cristiano, MDLS, Fausto, R. 2008. Photochemistry of
Tetrazole Derivatives in Cryogenic Rare Gas Matrices. In: Alejandro Sánchez, Sergio J. Gutierrez
(eds). Photochemistry Research Progress. Nova Science Publishers, Inc, NY, USA, Chapter 9, pp.
295-324. ISBN: 978-1-60456-568-3.
proceedings)
1. Araújo, NCP, O’Neill, PM, Cristiano, MLS. 2008. Design and Synthesis of Plasmodium DNADirected Endoperoxides. Revista Portuguesa de Farmácia L-II(3): 40.
2. ringela, A, Almeida, R, Araújo, NCP, Veríssimo, E, O’Neill, PM, Cristiano, MLS. 2008. Synthesis
of New Peptidic Cysteine Protease Inhibitors. Revista Portuguesa de Farmácia L-II(3): 177.
. Cabral, L, Araújo, NCP, O’Neill, PM, Cristiano, MLS. 2008. Synthesis of new DNA-directed prodrugs as potential antimalarial agents. Revista Portuguesa de Farmácia L-II(3): 178.
PhD THESES
Frija, LM. 2008. Investigation of structural effects on the reactivity of heterocyclic bioactive
compounds. PhD thesis, University of Algarve. Supervisor: Nuna Araújo, in collaboration with
Paul M. O'Neill (University of Liverpool).
The group maintains collaborations with:
-
University of Liverpool, UK, Departments of Chemistry and Pharmacology, Liverpool
School of Tropical Medicine
-
University of Coimbra, Department of Chemistry
244
-
University of La Plata, Argentina, Center for Research and Development in Food
Cryotechnology (CIDCA)
-
Jagiellonian University, Poland, Faculty of Chemistry, Ingardena
Publications reflect the international collaborations.
Within the ARTEMIP project, collaborations are also set with:
-
University of Leiden, Netherlands
-
University of Goteborg, Sweden
-
World Health Organization (WHO/TDR) Geneva
8e. Future research
1. Objectives
For this year, in the area of medicinal chemistry, the aim is to pursue research leading to the
development of hybrid drugs for malaria chemotherapy, combining an endoperoxide
pharmacophore and a DNA binder with affinity to the G-quadruplexes of the plasmodium
telemeres.
In the area of reactivity, structural studies on derivatives of heterocyclic bioactive compounds
such as tetrazole and benzisothiazole, and investigation of their reactivity, in view of developing
synthetic methodologies, will continue.
2.

Current funding
172.480,00 EUR, from the ARTEMIP project.

119.004,00 EUR, FCT PTDC/SAU-FCB/103085/2008, A multi-target therapeutic strategy to
overcome the selection of resistance to antimalarial drugs and drug-candidates.
245
9. OTHER LA ACTIVITIES
9a. Services and resources
1. Internal services and resources (Sharing of equipment and other resources both within
the LA, between LAs or with other institutions of Higher Education nationally or
internationally)
CIMAR LA occupies two distinct poles, one at the University of Porto (ca 3500 m2) and another
one at the University of Algarve (ca 1800 m2). Each pole also includes facilities (1000 m2) with
water closed circuits for animal maintenance with an overall capacity of 60000 liters of seawater
and freshwater. CIMAR researchers have also access to an aquaculture experimental station
located in the Algarve (ca 2 ha). CIMAR acquired two small vessels (12 and 17 meters) that will
be completely operational for coastal and estuarine campaigns during 2009.
In 2008, significant efforts have been made in the planning of a new building, to be constructed
within the Park of Science and Technology of the University of Porto.
In the last years, significant equipment was acquired through the national competitive program
for the scientific re-equipment launched by FCT (2 million EUR), mostly for analytical chemistry
and molecular and genetic research. The last items were purchased in 2008. The equipment
includes:
-
Integrated system for automating behaviour experiments and data collection
-
Complete set-up of intra- and extra-cellular electrophysiological recording
-
High Pressure Liquid Chromatography System with light scattering detector (HPLC)
-
Mass detector for HPLC
-
Isotope ratio mass spectrometer (IRMS)
-
Gas chromatography with Mass Spectometry
-
Sequence detection system for Real Time PCR
-
Automatic DNA sequencers
-
Multipurpose liquid scintillation and luminescence counter
-
Upgrade of Image Analysis System
-
Phosphorimager and fluorimager
-
Culture chambers with light, temperature and photoperiod controls
-
Microplate Spectrofluorimeter
-
Gas Chromatography coupled to Mass Spectrometry (GC-MS)
246
-
Gamma Counter
-
Ultracentrifuge, rotors and accessories
-
Epi-fluorescence and phase contrast photomicroscope
-
Sequence Detection System for Real-time Quantitative PCR
-
Inverted Microscope for transmitted light, flurorescence, Nomarski DIC and Phasecontrast
-
Cryostat
-
Laminar flow cabinet
-
Multianaliser FIA and Underwater autoanalyser
-
Cell culture facility
-
Microplate reader
-
Atomic Absorption Spectrometer
This equipment is shared within CIMAR but is also available to other LAs and Higher Educations
Institutions. CIMAR can also access to equipment from other LA and Higher Education
Institutions mainly for scanning and transmission electron microscopy. In the framework of the
agreement between FCT and the Portuguese Hydrographic Institute, CIMAR researchers have
also the possibility to hire ship time at low price.
Other resources shared with national and international institutions include: Cell culture facilities
and cell lines (e.g. University of Bergen, NIFES, University of Ghent), zebrafish culture facilities
(IMM-Institute for Molecular Medicine, Lisbon).
Two services interface have been operating within the LA, for analytical chemistry and
molecular and genetic research. Several services are installed in both centers for administrative
and technical support, with a total of 23 staff members, including public and international
relations, project management, finance, IT systems, image and communication, juridical,
biotery, and maintenance.
Through several protocol agreements with higher education institutions, CIMAR has been
supporting pre and post graduation training by providing facilities and resources including the
participation of non-academic staff in teaching activities.
2. External services and resources (Scientific or technical services provided both nationally
or internationally to Governments/Institutions or the community)
CIMAR has been involved in a large number of activities to support public policies. Most relevant
are:
-
Member of European Science Foundation – Marine Board.
247
-
Third party of FCT in the ERA-nets AMPERA and Biodiversa, FP6.
-
Detachment of a permanent staff element to integrate the Portuguese network of FP7
National Contacts Points, supported by the Minister of Science and Technology, to
provide assistance to potential FP7 participants (Universities, Research Centers, SMEs,
Industry) in all aspects regarding the “Environment, including Climate Change” Theme.
-
Founding member of the Institute for the Development of the Knowledge and the
Economy of the Sea (IDCEM - Agency for the promotion of scientific knowledge,
valorization of research and technology service agreements, and promotion of
entrepreneurship).
-
Contribution to the implementation of the Ocean Agenda of the North Region, North
Regional Coordination and Development Commission (CCDR-N); Leadership of the
Ocean agenda for the territorial cooperation North of Portugal – Galicia.
-
Contribution to the Regional Spatial Planning of Algarve, CCDR-Algarve.
-
Contribution to the implementation of the Water Framework Directive, National
Institute for Water (INAG).
Moreover, CIMAR has been involved in several R&D projects aimed to give support to European
Territorial cooperation (eg Interreg) and has been regularly requested by the government to
give advice on legislation (eg. Environment and fishing) and bilateral agreements involving
Portugal.
At the individual level, members of the Direction of CIMAR have also been involved in several
activities:
-
Member of the scientific council of FCT.
-
National delegate for FP7.
-
Director of IDCEM.
-
Member of the Council of the Task Group for Maritime Affairs (EMAM).
-
Focal Point of the Minister of Science and Technology on the National Ocean Strategy.
-
Ambassador of the Commissioner Joe Borg (DG Mare) for the dissemination of the
Green Book on the European Maritime Policy.
CIMAR provided services for the industry in several areas including water quality (chemical,
biological and toxicological), diet formulation for aquaculture, DNA extraction and genotyping,
physical oceanographic data collection and environmental consulting studies.
Both CIIMAR and CCMAR have been qualified for providing R&D and innovation services to SMEs
under the Portuguese National Strategic Reference Framework (QREN, 2007-2013).
248
9b. Networking actions
1. Networking actions (Both national and international activities)
CIMAR members have been involved in numerous network actions to build the National and
European Research Areas. Most relevant:
-
Member of European Science Foundation – Marine Board (www.esf.org/researchareas/marine-board.html)
-
EU Networks of Excellence - FP6
MGE - Marine Genomics Europe (www.marine-genomics-europe.org) and, as an
outcome, ASSEMBLE - Association of European Marine Biological Laboratories (EU FP7
Infrastructure proj, www.assemblemarine.org)
MARBEF - Marine Biodiversity and Ecosystem Function (www.marbef.org)
-
ERA Nets - FP6 (Third party of FCT)
BiodivERsA (www.eurobiodiversa.org)
AMPERA (www.cid.csic.es/ampera)
-
Technological Platforms
European Aquaculture Technology Platform (www.eatpnet.eu)
ASEM Aquaculture Platform (www.asemaquaculture.org)
Water Supply and Sanitation Technology Platform (www.wsstp.eu)
-
ESF Networking Programmes
Functional Dynamics in Complex Chemical and Biological Systems (http://funcdyn.org)
Molecular Simulations in Biosystems and Material Science (www.simbioma.cecam.fr)
-
COST Actions (www.cost.esf.org)
Improved constrains on models of glacial isostatic adjustment, ES0701
Welfare of fish in European aquaculture - n. 867
Critical success factors for fish larval production in European Aquaculture: a
multidisciplinary network - n. FA0801
-
International Thematic Networks
European Network of Marine Institutes and Stations (www.marsnetwork.org)
249
EPBRS (www.epbrs.org), and the supportive networks BioPlatform (CIMAR coordination,
www.bioplatform.info) and Biostrat
Historical
Ecology
of
the
(www.biology.duke.edu/corona)
Trans-Atlantic
MedZoo
Network,
through
(www.ciesm.org/marine/programs/zooplankton.htm)
Marine
Biota
CIESM
Network,
program
Working Group on Habitat Mapping, ICES (www.ices.dk)
Red Iberoamericana de Toxicología y Seguridad Química (http://ritsq.org)
Rede Iberoamericana de Contaminação e Toxicologia Ambiental, SICTA
-
National Thematic Network
DivMar - Rede de Biodiversidade Marinha e Costeira (www.cimar.org/divmar)
-
Training Network
European Erasmus Network in Aquaculture, Fisheries & Aquatic Resources
Management, (www.aquatnet.com)
-
FP7 National Contact Points Network
CIMAR has detached a permanent staff element to integrate the Portuguese network of
NCP for FP7 (Environment theme)
9c. Training
1. Training activities (Activities of the LA as a whole)
In 2008, 57 MSc students and 26 PhD students completed their degree under (co)-supervision of
CIMAR members. New theses are currently underway.
Pre-graduation Studies
-
40 undergraduated students were incorporated in R&D project teams, with Integration
into Research Grants (BII), to foster scientific activity and the development of critical
thinking skills, creativity and autonomy.
Post-graduation Studies
-
250
Doctoral School in Marine and Environmental Sciences, in cooperation with CESAM,
University of Porto and University of Aveiro; with four areas of specialization: Marine
Resources and Aquaculture, Oceanography and Marine Ecosystems; Environmental
Quality; Environmental Planning and Management.
Involvement in European and National MSc programmes:
-
Science in Marine Biodiversity and Conservation (www.embc.marbef.org)
-
Ecological Management of Catchments (www.ecocach.eu)
-
Sciences of the Sea – Sea Resources, University of Porto
-
Environmental Contamination and Toxicology, University of Porto
-
Aquaculture and Fisheries, University of Algarve
Organization of advanced training courses, workshops and internal seminars series. Most
relevant:
-
Bayesian Evolutionary Analysis Sampling Trees – BEAST, 29-30 May 2008, Faro.
-
Molecular Techniques for Phylogeny and Biogeography of Macroalgae, 8-12 September
2008, Faro.
-
Workshop in Microarray; in the context of AQUAGENOME project (FP6, EU), March
2008, Gothenburg, Sweden.
-
Science and Illustraion Course, 24-30 November 2008, Faro.
-
Internal seminars in Faro and Porto on a fortnight basis
9d. Outreach/science and society
1. Outreach/science and society (Activities that the LA as a whole)
CIMAR has become increasingly committed to outreach activities to foster the dialogue between
scientists and society, to improve the public knowledge and perception of science and to
increase the interest of the young students towards scientific careers. Some of the most
relevant outreach activities include:
Scientific management of public equipment
-
CMIAs - Environmental Monitoring and Interpretation Centres
Through protocols with the municipalities of Matosinhos and Vila do Conde (www.cmiaviladoconde.net), CIMAR is directly managing two CMIAs, which opened in 2007 (15000
visitors). Both centres have displayed several temporary exhibitions, organized
conferences and professional courses and performed regular in house and field
activities for public of different ages, with a particular emphasis on young students.
-
Public aquariums and fisheries museums
251
Aguda Littoral Station - ELA (www.fundacao-ela.pt, ca 50000 visitors/year) and River
Minho AQUAMuseum (www.cimsoft.pt/aquamuseu, ca 30000 visitors/year) have been
created and managed by CIMAR researchers. Both stations have a special relevance in
what concerns Environmental Education Programs for all age.
-
Participation at the International Polar Year Education & Outreach Programme
LATITUDE60! (http://latitude60.blogspot.com)
The IPY has a strong education and outreach programme and the Portuguese
educational project LATITUDE60! is considered as one of the most active and
comprehensive projects internationally. More than 40 activities dealing with education
and outreach were developed to explain polar research, involving more than 200
schools, 400 teachers and thousands of students. The top events include science and art
competitions for schools culminating in an educational expedition of 7 high school
students integrated in “Students on ice” to Antarctica; itinerant exhibitions and acting,
workshops, lectures, pamphlets and books (also in Braille) and the Polar Science
Weekend at Pavilhão do Conhecimento (31 May-1 June, 6500 visitors).
-
Participation in the Ciência Viva Programme (http://www.cienciaviva.pt)
Several types of activities have been implemented at CIMAR, to contribute to the
promotion of a scientific and technological culture among the Portuguese population
and more specifically to encourage the use of experimental methods in science learning,
involving both scientific and school communities. Most relevant activities include:
-
-
Scientists go to school: CIMAR research groups, in collaboration with the Algarve
Ciência Viva Centre, have prepared a number of scientific themes to present at
elementary and high schools of Algarve.
-
Science on Holidays for Young People: CIMAR research groups prepared 22
scientific activities (1-2 weeks each) aiming to offer high school students the
opportunity for first-hand contact with the reality of scientific research work
during their summer vacations (students from Spain have also been involved in
2008).
-
Biology Activities in the Summer: CIMAR prepared field activities (4-8 hours
each) for general public of all ages, in particular family groups.
Visionarium - Europarque Science Centre
CIMAR in collaboration with the Visionarium - Europarque Science Centre, is involved in
a national project using Daphnia as biological model for experimental teaching of
sciences in high schools. This project involves more 220 schools, 320 teachers and 2500
students (2008-2009). (http://projectodaphnia0.blogs.sapo.pt/).
252
-
-
Participation in other events and exhibitions
-
Junior University (http://universidadejunior.up.pt/), within the School of Life
and Health Sciences for high school students.
-
“Science, Education and Innovation” exhibition by the University of Porto, April
2008, through the presentation of the research developed at CIMAR to
encourage high school students to engage in scientific careers.
-
EXPOMAR exhibition, 2008, Exposition about ecology of bivalves and Workshop
on aquaculture of new species.
-
Several seminars to the general public and participation of volunteers within
the scope of scientific projects (i.e. Biomares, Acidbiv, FindKelp, Seacase).
CIMAR in the media
CIMAR activities have been also disseminated through the media: publications in
international non-scientific journals (i.e. Science Daily, CORDIS News, Science for
Environmental Policy-DG Environment News Alert Service) and newspapers (i.e. Público,
El País, Diário de Notícias), interviews on radio station (i.e. RDP África) and participation
in TV programs.
9e. International events
1. Organization of international events (Events that are international in dimension and
required the involvement of the LA for their implementation)
CIMAR organized several international scientific congresses and workshops. Most relevant
included:
-
13th International Symposium on Nutrition and Feeding of Fish, 1-5 June, Florianopolis,
Brazil.
-
1st Portuguese Conference on Cyanotoxins, 7-8 July, Porto, Portugal.
-
International Congress Seasink 2008. University Fernando Pessoa, 26-28 July, Porto,
Portugal.
-
International Workshop Geophysical Fluid Dynamics: Island Wakes Studies, 21-31 July,
Funchal, Portugal. 29 participants.
-
International workshop on Telemetry-Theory, Concepts and Applications, 29 September,
Faro, Portugal. 29 participants.
-
International workshop on Acoustic Telemetry- Equipments and Demonstrations, 30
September, Faro, Portugal. 29 participants.
253
-
between Concepts and Measurements, 30 March-8 April, Eilat, Israel.
-
4th Workshop on the Cultivation of Soles, 11-13 November, Faro, Portugal. 80
participants.
Internal Seminars Series at CIIMAR and CCMAR, all year.
Organization of major international conferences and workshops programmed for 2009:
-
International Workshop on Approaches in Fish Skeletal Biology, 27-29 April 2009,Tavira,
Portugal. 100 participants from 12 countries.
-
1st Iberian Conference on Cyanotoxins,
(http://www.cimar.org/cic2009/)
-
6th European Conference on Marine Natural Products, 17-23 July 2009, Porto, Portugal.
(http://www.cimar.org/6ECMNP/)
-
7th AIEC Congress, Iberian Association of Comparative Endocrinology, 6-9 September
2009, Porto, Portugal. (http://www.ciimar.up.pt/7aiec/index.html)
-
BIOLIEF, World conference on Biological Invasions and Ecosystem Functioning, 27-30
October 2009, Porto, Portugal. (http://www.ciimar.up.pt/biolief/)
254
6-7
July
2009,
Porto,
Portugal.
10. INTERNAL EVALUATIONS
10.1 Summary of internal evaluations during 2008
A member of the External Advisory Board, Prof. Steve Hawkins, visited CIMAR in August 2008.
The advice and discussions centred on promoting research capability in the general area of
marine biodiversity and ecosystems science. As a result of this visit, it was decided to
reformulate the organization of the recently created research group on Biodiversity of Aquatic
Ecosystems. The identification of existing gaps were crucial for the definition of the five
positions for Assistant Researchers at the postdoctoral level in the area of Marine Biodiversity
and Ecosystem Functioning, that were opened in Aug-Sept 2008 within the Ciência 2008
initiative.
10.2 Future internal evaluations programmed for 2009
A meeting of the External Advisory Board is schedule for the fall 2009.
255
11. FUTURE OBJECTIVES
11.1 Summary of proposed future objectives
Future objectives of CIMAR LA reflect the multidisciplinary nature of Marine Sciences and are
aimed at consolidating existing lines of research, synergistic interactions of research teams, and
integrating new approaches that may contribute to a holistic view of marine ecosystems,
responsiveness to changes of natural or anthropogenic origin and contribution to social,
educational and economic sectors.
Specific objectives are:
-
Develop and contribute to integrate marine biodiversity initiatives and databases at a
national and European level through research networks.
-
Develop genomics and post-genomics capabilities to understand biogeochemical
processes, short and long-term adaptation including species invasions, extreme
environments, analysis of historical samples and potential biotechnological applications
such as marine biofuels.
-
Develop physical oceanography and modelling competences which can be associated
with existing remote sensing and biology expertise to understand ecosystem dynamics,
dispersion of pollutants and long term climatic variations.
-
Establish risk management procedures for natural toxins and xenobiotics by integrating
information from multiples levels of biological organization from molecules to
ecosystem.
-
Integrate social sciences in the evaluation of ecosystem services including fisheries.
-
Develop analytical chemistry and bioscience platforms (“omics”, imaging) to support the
LA and provide external services.
-
Stimulate technology transfer, setting up of spin offs, startups and partnerships with
industry, in particular aquaculture.
-
Develop an action plan to improve and stimulate cooperation between different
research groups within research lines and across research lines with the aim of reducing
fragmentation and stimulating cooperation.
-
Further increase the national and foster the international attractiveness of the PhD and
MSc programmes in which the CIMAR LA is an institutional partner.
-
Promote public understanding of sciences for the general public educational
programmes targeting schools at all levels.
-
Strive to improve cost-effectiveness of research and reduce waste and carbon footprint.
256
SCIENTIFIC OUTPUT 2008
A.
PUBLICATIONS
EDITED BOOKS
1.
Afonso-Dias, I, Menezes, G, MacKenzie, K, Eiras, JC. 2008. Proceedings of the International
Workshop on Marine Parasitology: Applied Aspects of Marine Parasitology. Arquipélago, Life and
Marine Sciences, Supplement 6, xiv + 49 p.
2.
Cabrita, E, Robles, V, Herraez, MP. 2008. Methods in Reproductive Aquaculture: Marine and
Freshwater Species. CRC Press, Boca Raton, 549 p. ISBN 978-0-8493-8053-2.
3.
Calado, R. 2008. Marine Ornamental Shrimp - Biology, Aquaculture and Conservation. WileyBlackwell, UK, 280 p. ISBN 978-1-4051-7086-4.
4.
Canário, AVM, Scott, AP, Hubbard, PC, Barata, EN. 2008. Bioactive water-borne chemicals:
pheromones and welfare indicators - the Faro Workshop. Special Issue Behaviour, Volume 145,
Number 10.
5.
Eiras, JC, Segner, H, Wahli, T, Kapoor, B. 2008. Fish Diseases. Science Publishers Inc., Enfield, NH,
USA, 2 Vol., 1308 p.
6.
Gonçalves, JMS, Oliveira, F, Coelho, R, Veiga, P, Erzini, K. 2008. Roteiros do mar: região de Vila
Real de St. António. Câmara Municipal de VRSA, Universidade do Algarve, Faro, 110 p.
7.
Rocha, MJ, Arukwe, A, Kapoor, BG. 2008. Fish Reproduction. Science Publishers Inc., Enfield, New
Hampshire, USA; 629 p. ISBN 978-1-57808-331-2.
8.
Sundell, K, Power, DM. 2008. The use of molecular methods and functional genomics in
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Vieira, R, Pereira, R, Arenas, F, Araújo, R, Sousa Pinto, I. 2008. Guia de Campo das Espécies
Intertidais Características da Costa Norte de Portugal – Praias Rochosas. CIMAR, Porto, Portugal.
10. Weber, M, Santos, A, Ferreira, A. 2008. Descobrir o Mar. Edições Afrontamento, Porto, Portugal,
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Azevedo, C. 2008. A Célula. In: Quintas, A, Freire, AP, Halpern, MJ (Eds). Bioquímica: Organização
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Azevedo, C. 2008. Matrix estrutural dos sistemas biológicos - Parte III. In: Quintas, A, Freire, AP,
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Brown, PB, Kaushik, S, Peres, H. 2008. Protein feedstuffs originating from soybeans. In: Lim, CE,
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Cabrita, E, Robles, V, Herráez P. 2008. Sperm quality assessment. In: Cabrita, E, Robles, V,
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Cabrita, E, Robles, V, Sarasquete C. and Herráez P. 2008. Sperm cryopreservation from the
marine Teleost, Sparus aurata. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in
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Conceição, LEC, Morais, SJ, Dinis, MT, Rønnestad, I. 2008. Tracer studies in fish larvae. In: Cyrino,
JEP, Bureau, D, Kapoor, BG (Eds). Feeding and Digestive Functions in Fishes. Science Publishers,
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Dias, S, Sousa, R, Lobón-Cerviá, J, Laffaille, P. 2008. The decline of diadromous fish in Western
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Dinis, MT, Soares, F, Cabrita, E. 2008. Optimização da reprodução em linguado (Solea
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Fernandes, C, Fontaínhas-Fernandes, A, Salgado, MA. 2008. Metabolic mechanisms of adaptation
to chronic metal exposure - a case study. In: Brown, SE, Welton, WC (Eds). Heavy Metal Pollution.
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Workshop for the analysis of the impact of Perkinsosis to the European shellfish Industry. Centro
de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos da Xunta de Galicia,
Vilanova de Arousa, Spain. Centro Tecnológico del Mar - Fundación CETMAR, Vigo, Spain. Legal
deposit: VG- 274-2008.
11. Gomez-Zavaglia, A, Reva, ID, Frija, LMT, Cristiano, MDLS, Fausto, R. 2008. Photochemistry of
tetrazole derivatives in cryogenic rare gas matrices. In: Sánchez, A, Gutierrez, SJ (Eds).
Photochemistry Research Progress. Nova Science Publishers Inc., NY, USA, Chapter 9, pp. 295324. ISBN 978-1-60456-568-3.
12. Gravato, C, Faria, M, Alves, A, Guilhermino, L. 2008. Biomonitoring studies performed with
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Kim, JY, Platt, U (Eds). Advanced Environmental Monitoring. Springer, Dordrecht. ISBN 978-14020-6363-3 (HB), ISBN 978-1-4020-6364-0 (e-book).
13. Hazin, H, Erzini, K. 2008. Análisis de series temporales multivariantes cortas: aplicación de MAFA
(Análisis de autocorrelación min/max factorial) y DFA (análisis dinámico factorial) a las CPUE de
palangreros Brasileños e índices climáticos. In: Gutiérrez Estrada, JC, Yáñez, Y. (Eds.). Nuevas
Aproximaciones Metodológicas para el Análisis de Pesquerías, Capitulo 9. ISBN 978-84-96826-557.
14. Herráez, P, Robles, V, Cabrita, E. 2008. Rainbow trout (Oncorhynchus mykiss) sperm
cryopreservation. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive
Aquaculture: Marine and Freshwater Species. CRC Press, Boca Raton, pp. 385-389. ISBN 978-08493-8053-2.
15. Lahnsteiner, F, Soares, F, Ribeiro, L, Dinis, MT. 2008. Egg quality determination in teleost fish. In:
Cabrita, E, Robles, V, Herráez, MP. (Eds). Methods in Reproductive Aquaculture: Marine and
Freshwater Species. CRC Press, Boca Raton, pp. 149-180. ISBN 978-0-8493-8053-2.
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an Integrative Approach. Cambridge University Press, pp. 132-173. ISBN 978-0-521-83243-4.
17. Peres, H, Lim, C. 2008. Use of soybean products in non-salmonids marine finfish. In: Lim, CE,
Webster, CD, Lee, CS (Eds). Alternative Protein Sources in Aquaculture Diets. Haworth Press, New
York, pp. 281-312.
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Larval Physiology. Science Publishers, Enfield, UK, Chapter 19, pp. 1-33.
19. Prouzet, P, Bouvet, J-C, Bru, N, Duquesne, E, Antunes, C, Damasceno-Oliveira, A, Boussouar, A,
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d'Abondance et de Colonization. Éditions Quae, Versailles, France. ISBN 978-2-7592-0085-6.
20. Robles, V, Cabrita, E, Acker, JP, Herráez, P. 2008. Embryo cryopreservation: what we know until
now. In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive Aquaculture: Marine
and Freshwater Species. CRC Press, Boca Raton, pp. 265-294. ISBN 978-0-8493-8053-2.
21. Robles, V, Cabrita, E, Herráez, P. 2008. Sperm cryopreservation of sex-reversed rainbow trout
(Oncorhynchus mykiss). In: Cabrita, E, Robles, V, Herráez, MP (Eds). Methods in Reproductive
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(Eds). Ecological Engineering, Vol. 2 of Encyclopedia of Ecology, Oxford, Elsevier, pp. 1413-1422.
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PUBLICATIONS IN INTERNATIONAL PEER REVIEWED JOURNALS
1.
Abbink, W, Kulczkowska, E, Kalamarz, H, Guerreiro, PM, Flik, G. 2008. Melatonin synthesis under
calcium constraint in gilthead sea bream (Sparus auratus L.). General and Comparative
Endocrinology 155: 94-100. IF=2.654.
2.
Abecasis, D, Bentes, L, Coelho, R, Correia, C, Lino, PG, Monteiro, P, Gonçalves, JMS, Ribeiro, R,
Erzini, K. 2008. Ageing seabreams: comparative study between scales and otoliths. Fisheries
Research 89: 37-48. IF=1.434.
3.
Abecasis, D, Erzini, K. 2008. Site fidelity and movements of gilthead sea bream (Sparus aurata) in
a coastal lagoon (Ria Formosa, Portugal). Estuarine, Coastal and Shelf Science 79: 758-763.
IF=2.072.
4.
Abrantes, F, Aft-Epping, U, Lebreiro, S, Voelker, A, Schneider, R. 2008. Tsunamis sedimentological
record on shallow shelf areas: the cases of 1969 AD and 1755 AD on the Portuguese Shelf off
Lisbon. Marine Geology 249: 283-293. IF=2.100. (*)
5.
Agüero-Chapin, G, Antunes, A, Ubeira, F, Chou, KC, González-Díaz, H. 2008. Comparative study of
topological indices of macro/supra-molecular RNA complex networks. Journal of Chemical
Information and Modeling 48(11): 2265-2277. IF=3.643.
6.
Alberto, F, Massa, S, Manent, P, Diaz-Almela, E, Arnaud-Haond, S, Duarte, CM, Serrão, EA. 2008.
Genetic differentiation and secondary contact zone in the seagrass Cymodocea nodosa across the
Mediterranean-Atlantic transition region. Journal of Biogeography 35: 1279-1294. IF=4.566.
7.
Almeida, C, Coelho, R, Silva, M, Bentes, L, Monteiro, P, Ribeiro, J, Erzini, K, Gonçalves, JMS. 2008.
Use of different intertidal habitats by faunal communities in a temperate coastal lagoon.
8.
Almeida, CMR, Mucha, AP, Bordalo, AA, Vasconcelos, MTSD. 2008. Influence of a salt marsh plant
(Halimione portulacoides) on the concentrations and potential mobility of metals in sediments.
Science of the Total Environment 403(1-3): 188-195. IF=2.579.
9.
Almeida, CMR, Mucha, AP, Delgado, MFC, Caçador, IM, Bordalo, AA, Vasconcelos, MTSD. 2008.
Can PAHs influence Cu accumulation by salt marsh plants?. Marine Environmental Research
66(3): 311-318. IF=2.032.
10. Almeida, R, Gomez-Zavaglia, A, Kaczor, A, Cristiano, MLS, Eusebio, MES, Maria, TMR, Fausto, R.
2008. First observation of Chapman rearrangement of a pseudosaccharyl ether in the solid state:
the thermal isomerization of 3-(methoxy)1,2-benzisothiazole 1,1-dioxide revisited. Tetrahedron
64: 3296-3305. IF=2.897.
11. Antunes, A, Troyer, JL, Roelke, ME, Pecon-Slaterry, J, Packer, C, Winterbach, C, Winterbach, H,
Hemson, G, Frank, L, Stander, P, Siefert, L, Driciru, M, Funston, PJ, Alexander, K, Prager, KC, Mills,
, Wildt, D, ush, M, O’ rien, SJ, Johnson, WE. 2008. The evolutionary dynamics of lion Panthera
leo revealed by host and viral population genomics. PLoS Genetics 4(11): e1000251. IF=8.883.
12. Antunes, P, Hendriks, AJ, Huijbregts, MAJ, Gil, O, Reis-Henriques, MA. 2008. Organ-specific
accumulation and elimination patterns of PCBs in adult seabass (Dicentrarchus labrax). Science of
Total Environment 407: 204-210. IF=2.579.
13. Aragão, C, Corte-Real, J, Costas, B, Dinis, MT, Conceição, LEC. 2008. Stress response and changes
in amino acid requirements in Senegalese sole (Solea senegalensis Kaup 1858). Amino Acids 34:
143-148. IF=4.132.
14. Araújo, IB, Pugh, DT. 2008. Sea Levels at Newlyn 1915-2005: Analysis of trends for future flooding
risks. Journal of Coastal Research 24(sp3): 203-212. IF=0.517.
15. Araújo, R, Cabral, JP, Rodrigues, AG. 2008. Air filtration systems and restrictive access conditions
improve indoor air quality in clinical units: Penicillium as a general indicator of hospital indoor
fungal levels. American Journal of Infection Control 36: 129-134. IF=2.678.
16. Aureliano, M, Henao, F, Baruah, B, Crans, DC. 2008. Sarcoplasmic reticulum calcium ATPase is
inhibited
by
organic
vanadium
coordination
compounds:
pyridine-2,6dicarboxylatodioxovanadium(V), BMOV, and an amavadine analogue. Inorganic Chemistry 47:
5677-5684. IF=4.147.
17. Ávila, SP, Madeira, P, da Silva, CM, Cachão, M, Landau, B, Quartau, R, Martins, AMF. 2008. Local
disappearance of bivalves in the Azores during the last glaciation. Journal of Quaternary Science
23(8): 777-785. IF=2.976.
18. Azenha, MA, Evangelista, R, Martel, F, Vasconcelos, MTSD. 2008. Estimate of the digestibility,
assimilability and intestinal permeability of butyltins occurring in wine. Food and Chemical
Toxicology 46(2): 767-773. IF=2.321.
19. Azevedo, C, Casal, G, Matos, P, Matos, E. 2008. A new species of Myxozoa, Henneguya rondoni n.
sp. (Myxozoa), from the peripheral nervous system of the Amazonian fish, Gymnorhamphichthys
rondoni (Teleostei). Journal of Eukaryotic Microbiology 55: 229-234. IF=1.502.
20. Azevedo, C, Casal, G, Montes, J. 2008. Ultrastructural developmental cycle of Haplosporidium
montforti (Phylum Haplosporidia) in its farmed abalone host, Haliotis tuberculata (Gastropoda).
Journal of Parasitology 94: 137-142. IF=1.165.
21. Azevedo, I, Duarte, PM, Bordalo, AA. 2008. Understanding spatial and temporal dynamics of key
environmental characteristics in a mesotidal Atlantic estuary (Douro, NW Portugal). Estuarine,
Coastal and Shelf Science 76: 620-633. IF=2.072.
Pollution 151(2): 408-413. IF=3.135.
23. Barata, EN, Fine, JM, Hubbard, PC, Almeida, OG, Frade, P, Sorensen, PW, Canario, AVM. 2008. A
sterol-like odorant in the urine of Mozambique tilapia males likely signals social dominance to
females. Journal of Chemical Ecology 34: 438-449. IF=2.327.
24. Barata, EN, Serrano, RM, Miranda, A, Nogueira, R, Hubbard, PC, Canario, AVM. 2008. Putative
pheromones from the anal glands of male blennies attract females and enhance male
reproductive success. Animal Behaviour 75: 379-389. IF=2.828.
25. Barata, M, Harris, DJ, Castilho, R. 2008. Variation within North African Natrix maura (Serpentes:
Colubridae) inferred from mtDNA sequences: comparative phylogeography of Magrehbian
reptiles. African Zoology 43: 1-7. IF=0.437.
26. Barrento, S, Marques, A, Pedro, S, Vaz-Pires, P, Nunes, ML. 2008. The trade of live crustaceans in
Portugal: space for technological improvements. ICES Journal of Marine Science 65: 551-559.
IF=1.661.
27. Barrento, S, Marques, A, Teixeira, B, Vaz-Pires, P, Nunes, ML. 2008. Essential elements and
contaminants in edible tissues of European and American lobsters. Food Chemistry 111: 862-867.
IF=2.696.
28. Bartilotti, C, Calado, R, dos Santos, A. 2008. Complete larval development of the hermit crabs
Clibanarius aequabilis and Clibanarius erythropus (Decapoda: Anomura: Diogenidae), under
laboratory conditions, with a revision of the larval features of genus Clibanarius. Helgoland
Marine Research 62: 103-121. IF=1.591.
29. Batista, FM, Ben-Hamadou, R, Fonseca, VG, Taris, N, Ruano, F, Henriques, MA, Boudry, P. 2008.
Comparative study of shell shape and muscle scar pigmentation in the closely related cupped
oyster taxa Crassostrea angulata, C. gigas and their reciprocal hybrids. Aquatic Living Resources
21: 31-38. IF=0.667.
30. Beirão, J, Cabrita, E, Soares, F, Herraez, MP, Dinis, MT. 2008. Cellular damage in spermatozoa
from wild-captured Solea senegalensis as detected by two different assays: comet analysis and
Annexin V-Fluorescein staining. Journal of Applied Ichthyology 24: 508-513. IF=0.638.
31. Bender, N, Heg-Bachar, Z, Oliveira, RF, Canario, AVM, Taborsky, M. 2008. Hormonal control of
brood care and social status in a cichlid fish with brood care helpers. Physiology & Behavior 94:
349-358. IF=2.806.
32. Bender, N, Taborsky, M, Power, D. 2008. The Role of prolactin in the regulation of brood care in
the cooperatively breeding fish Neolamorologus pulcher. Journal of Experimental Zoology Part A Ecological Genetics and Physiology 309A: 515-524. IF=1.549.
33. Bevelander, GS, Pinto, SLCP, Canario, AVM, Spanings, T, Flik, G. 2008. CYP27A1 expression in
gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related
protein. Journal of Endocrinology 196: 625-635. IF=2.791.
34. Bio, A, Couto, A, Costa, R, Prestes, A, Vieira, N, Valente, A, Azevedo, J. 2008. Effects of fish
removal in the Furnas lake, Azores. Arquipélago - Life and Marine Sciences 25: 77-87.
35. Boane, C, Cruz, C, Saraiva, A. 2008. Metazoan parasites of Cyprinus carpio L. (Cyprinidae) from
Mozambique. Aquaculture 284: 59-61. IF=1.678.
36.
orges, MT, Sousa, A, De Marco, P, Matos, A, H nigov , P, Castro, PML. 2008. Aerobic and anoxic
growth and nitrate removal capacity of a marine denitrifying bacterium isolated from a
recirculation aquaculture system. Microbial Ecology 55(1): 107-118. IF=2.885.
37. Bos, MS, Fernandes, RMS, Williams, SDP, Bastos, L. 2008. Fast error analysis of continuous GPS
observations. Journal of Geodesy 82(3): 157-166. IF=1.689.
38. Brun, FG, Olivé, I, Malta, E-J, Vergara, JJ, Hernández, I, Pérez-Lloréns, JL. 2008. Increased
vulnerability of Zostera noltii to stress caused by low light and elevated ammonium levels under
phosphate deficiency. Marine Ecology Progress Series 365: 67-75. IF=2.631.
39. Buszewski, B, Ligor, T, Filipiak, W, Vasconcelos, MTSD, Pompe, M, Veber, M. 2008. Study of
sorptive properties of trap systems for selective enrichment of volatile organic compounds from
tobacco smoke samples. Toxicological and Environmental Chemistry 90(1): 51-64.
40. Cabaço, S, Machas, R, Vieira, V, Santos, R. 2008. Impacts of urban wastewater discharge on
seagrass meadows (Zostera noltii). Estuarine, Coastal and Shelf Science 78: 1-13. IF=2.072.
41. Cabaço, S, Santos, R, Duarte, CM. 2008. The impact of sediment burial and erosion on seagrasses:
a review. Estuarine, Coastal and Shelf Science 79: 354-366. IF=2.072.
42. Cabral, JP. 2008. Gonçalo Sampaio and botanical taxonomy. Botanica Complutensis 32: 69-73.
43. Calado, R, Bartilotti, C, Goy, JW, Dinis, MT. 2008. Parasitic castration of the stenopodid shrimp
Stenopus hispidus (Decapoda: Stenopodidae) induced by the bopyrid isopod Argeiopsis inhacae
(Isopoda: Bopyridae). Journal of the Marine Biological Association of the United Kingdom 88:
307-309. IF=1.056.
44. Calado, R, Dinis, MT. 2008. Collection of marine invertebrates for the aquarium trade in
European waters: is anyone surveying?. Aquatic Conservation - Marine & Freshwater Ecosystems
18: 335-338. IF=1.500.
45. Calado, R, Dionisio, G, Bartilotti, C, Nunes, C, dos Santos, A, Dinis, MT. 2008. Importance of light
and larval morphology in starvation resistance and feeding ability of newly hatched marine
ornamental shrimps Lysmata spp. (Decapoda: Hippolytidae). Aquaculture 283: 56-63. IF=1.678.
46. Calado, R, Pimentel, T, Vitorino, A, Dionisio, G, Dinis, MT. 2008. Technical improvements of a
rearing system for the culture of decapod crustacean larvae, with emphasis on marine
ornamental species. Aquaculture 285: 264-269. IF=1.678.
47. Campos, J, Van der Veer, HW. 2008. Autecology of Crangon crangon (L.) with an emphasis on
latitudinal trends. Oceanography and Marine Biology - Annual Review 46: 65-104.
48. Canario, AVM, Bargelloni, L, Volckaert, F, Houston, RD, Massault, C, Guiguen, Y. 2008. Genomics
toolbox for farmed fish. Reviews in Fisheries Science 16: 3-15. IF=2.375.
49. Cancela da Fonseca, L, Cristo, M, Machado, M, Sala, J, Reis, J, Alcazar, R, Beja, P. 2008.
Mediterranean temporary ponds in Southern Portugal: key faunal groups as management tools?.
PANAMJAS 3: 304-320.
50. Carvalho, PN, Rodrigues, PNR, Alves, F, Evangelista, R, Basto, MCP, Vasconcelos, MTSD. 2008. An
expeditious method for the determination of organochlorine pesticides residues in estuarine
sediments using microwave assisted pre-extraction and automated headspace solid-phase
microextraction coupled to gas chromatography-mass spectrometry. Talanta 76: 1124-1129.
IF=3.206.
51. Casal, G, Matos, E, Matos, P, Azevedo, C. 2008. Ultrastructural description of a new myxosporean
parasite Kudoa aequidens sp. n. (Myxozoa, Myxosporea), found in the sub-opercular musculature
of Aequidens plagiozonatus (Teleostei) from the Amazon river. Acta Protozoologica 47: 135-141.
IF=1.013.
52. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2008. A new microsporidian parasite, Potaspora
morhaphis n. gen., n. sp. (Microsporidia) infecting the teleostean fish Potamorhaphis guianensis
from the river Amazon. Morphological, ultrastructural and molecular characterization.
Parasitology 135: 1053-1064. IF=2.071.
53. Catalao, J, Bos, MS. 2008. Sensitivity analysis of the gravity geoid estimation: A case study on the
Azores plateau. Physics of the Earth and Planetary Interiors 168(1-2): 113-124. IF=2.353.
54. Cerqueira, F, Cidade, H, van Ufford, L, Beukelman, C, Kijjoa, A, Nascimento, MSJ. 2008. The
natural prenylated flavone artelastin is an inhibitor of ROS and NO production. International
Immunopharmacology 8(4): 597-602. IF=2.157.
55. Chevaldonné, P, Sket, B, Marschal, C, Lejeusne, C, Calado, R. 2008. Improvements to the ‘sket
bottle’: a simple manual device for sampling small crustaceans from marine caves and other
cryptic habitats. Journal of Crustacean Biology 28: 185-188. IF=1.109.
56. Chicharo, MA, Chicharo, L. 2008. RNA:DNA ratio and other nucleic acid derived indices in Marine
Ecology. International Journal of Molecular Sciences 9: 1453-1471. IF=0.978.
57. Coelho, R, Erzini, K. 2008. Effects of fishing methods on deep water shark species caught as bycatch off southern Portugal. Hydrobiologia 606(1): 187-193. IF=1.449.
58. Coelho, R, Erzini, K. 2008. Identification of deep water lantern sharks (Chondrichthyes:
Etmopteridae) using morphometric data and multivariate analysis. Journal of the Marine
Biological Association of the United Kingdom 88: 199-204. IF=1.056.
59. Coelho, R, Erzini, K. 2008. Life history of a wide ranging deep water lanternshark in the NE
Atlantic, Etmopterus spinax (Chondrichthyes: Etmopteridae), with implications for conservation.
Journal of Fish Biology 73: 1419-1443. IF=1.246.
60. Coesel, SN, Baumgartner, AC, Teles, LM, Ramos, AA, Henriques, NM, Cancela, L, Varela, JCS.
2008. Nutrient limitation is the main regulatory factor for carotenoid accumulation and for Psy
and Pds steady state transcript levels in Dunaliella salina (Chlorophyta) exposed to high light and
salt stress. Marine Biotechnology 10: 602-611. IF=2.544.
61. Coesel, SN, Oborník, M, Varela, J, Falciatore, A, Bowler, C. 2008. Evolutionary origins and
functions of the carotenoid biosynthetic pathway in marine diatoms. PloS ONE 3(8): e2896.
602-611. IF=2.544.
63. Conceição, N, Laizé, V, Simões, B, Pombinho, AR, Cancela, ML. 2008. Retinoic acid is a negative
regulator of matrix Gla protein gene expression in teleost fish Sparus aurata. Biochimica
Biophysica Acta 1779: 28-39. IF=2.282.
64. Correia, M, Palma, J, Andrade, JP. 2008. Effects of live prey availability on growth and survival in
the early stages of cuttlefish Sepia officinalis (Linnaeus, 1758) life cycle. Aquaculture Research
39(1): 33-40. IF=0.991.
65. Correia, M, Palma, J, Kirakowski, T, Andrade, JP. 2008. Effects of prey nutritional quality on
growth and survival of juvenile cuttlefish, Sepia officinalis (Linnaeus, 1758). Aquaculture
Reasearch 39: 869-876. IF=0.991.
66. Costa, MC, Martins, M, Jesus, C, Duarte, JC. 2008. Treatment of acid mine drainage by sulphatereducing bacteria using low cost matrices. Water Air and Soil Pollution 189: 149-162. IF=1.398.
67. Costa, ME, Erzini, K, Borges, TC. 2008. Bycatch of crustacean and fish bottom trawl fisheries from
southern Portugal (Algarve). Scientia Marina 72: 801-814. IF=1.075.
68. Costa-Dias, S, Lobón-Cerviá, J. 2008. Diel feeding activity and intensity in European eel Anguilla
anguilla (L.) during an annual cycle in a Cantabrian stream. Knowledge and Management of
Aquatic Ecosystems 309-391: 1p1-1p9.
69. Costas, B, Aragão, C, Mancera, JM, Dinis, MT, Conceição, LEC. 2008. High stocking density induces
crowding stress and affects amino acid metabolism in Senegalese sole Solea senegalensis (Kaup
1858) juveniles. Aquaculture Research 39: 1-9. IF=0.991.
70. Couto, A, Enes, P, Peres, H, Oliva-Teles, A. 2008. Effect of water temperature and dietary
carbohydrate level on growth performance and metabolic utilization of diets in gilthead sea
bream (Sparus aurata) juveniles. Comparative Biochemistry and Physiology - Part A 151: 45-50.
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71. Cruz, C, Bio, AMF, Julliotti, A, Tavares, A, Martins-Loução, MA. 2008. Heterogeneity of soil surface
ammonium concentration and other characteristics, related to plant specific variability in a
Mediterranean-type ecosystem. Environmental Pollution 154: 414–423. IF=3.135.
72. Cruzeiro, L. 2008. Influence of the sign of the coupling on the temperature dependence of optical
properties of one dimensional exciton models. Journal of Physics B: Atomic, Molecular and
Optical Physics 41: 195401. IF=2.089.
73. Cruzeiro, L. 2008. Protein's multi-funnel energy landscape and misfolding diseases. Journal of
Physical Organic Chemistry 21: 549-554. IF=1.415.
74. Cunha, MC, Makridis, P, Soares, F, Rodrigues, P, Dinis, MT. 2008. Timing of appearance of
lymphoid cells during early development of senegalese sole, Solea senegalensis Kaup. Journal of
the World Aquaculture Society 39: 436-439. IF=0.693.
75. Cunha, RL, Tenório, MJ, Afonso, C, Castilho, R, Zardoya, R. 2008. Replaying the tape: recurring
biogeographical patterns in Cape Verde Conus after 12 million years. Molecular Ecology 17: 885901. IF=5.325.
76. Cunha, SR, Gonçalves, R, Silva, SR, Correia, AD. 2008. An automated marine biomonitoring
77. Da Costa, PM, Bica, A, Vaz-Pires, P, Bernardo, F. 2008. Effects of antimicrobial treatment on
selection of resistant Escherichia coli in broiler fecal flora. Microbial Drug Resistance 14: 299-306.
IF=1.800.
78. Da Costa, P, Vaz-Pires, P, Bernardo, F. 2008. Antimicrobial resistance in Escherichia coli isolated in
wastewater and sludge from poultry slaughterhouses wastewater plants. Journal of
Environmental Health 70(7): 40-45. IF=0.805.
79. Da Fonseca, R, Johnson, WE, O’ rien, SJ, Ramos, MJ, Antunes, A. 2008. The adaptive evolution of
the mammalian mitochondrial genome. BMC Genomics 9: 119. IF: 4.092
80. Davies, AJ, Amado, LL, Cook, RT, Bianchini, A, Eiras, JC. 2008. Potential environmental and host
ender influences on prevalence of Haemogregarina platessae (Adeleorina: Haemogregarinidae)
and suspected Haemohormidium terranovae (incerta sedis) in Brazilian flounder from the Patos
Lagoon Estuary, Southern Brazil. Folia Parasitologica 55: 161-170. IF=1.307.
81. Davies, SJ, Gouveia, A. 2008. Enhancing the nutritional value of pea seed meals (Pisum sativum)
by thermal treatment or isogenic selection in comparison with soybean meal for African catfish,
Clarias gariepinus. Aquaculture 283: 116-122. IF=1.678.
82. Dias, AS, Mills, RA, Taylor, RN, Ferreira, P, Barriga, FJAS. 2008. Geochemistry of a sediment pushcore from the Lucky Strike hydrothermal field, Mid-Atlantic Ridge. Chemical Geology 247(3-4):
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83. Diniz, DG, Varella, JEA, Guimarães, MDF, Santos, AFL, Fujimoto, RY, Monfort, KCF, Pires, MAB,
Martins, ML, Eiras, JC. 2008. A note on the occurrence of praniza larvae of Gnathia sp.
(Crustacea, Isopoda, Gnathiidae) on fishes from Northeast of Pará, Brazil. Anais da Academia
Brasileira de Ciências 80(4): 657-664. IF=0.881.
84. Domingues, P, Ferreira, A, Marquez, L, Andrade, JP, López, N, Rosas, C. 2008. Growth, absorption
and assimilation efficiency by mature cuttlefish (Sepia officinalis) fed with alternative and
artificial diets. Aquaculture International 16: 215-229. IF=0.608.
85. Dos Santos, A, Calado, R, Araujo, R. 2008. First record of the genus Periclimenaeus borradaile,
1815 (decapoda : palaemoniidae : pontoniinae) in the northeastern Atlantic, with the description
of a new species, Periclimenaeus aurae. Journal of Crustacean Biology 28: 156-166. IF=1.109.
86. Eiras, JC, Chemes, SB, Casabianca, OA, Takemoto, R, Rossi, LM, Pavanelli, GC. 2008. An anusual
infection of Henneguya sp. (Myxozoa, Myxosporea, Myxobolidae) in the gills of Hoplosternum
littorale Hancock, 1928 (Osteichtyhes, Callichthyidae) from Middle Paraná River, Santa Fe,
Argentina. Bulletin of the European Association of Fish Pathologists 28: 22-26. IF=0.430.
87. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2008. Henneguya caudicula n. sp. (Myxozoa,
Myxobolidae), a parasite of Leporinus lacustris (Osteichthyes, Anostomidae) from the high
Paraná River, Brazil, with a revision of Henneguya spp. infecting South American fish. Acta
88. Elandalloussi, LM, Leite, RB, Rodrigues, PM, Afonso, R, Cancela, ML. 2008. Effect of the herbicide
Roundup on Perkinsus olseni in vitro proliferation and in vivo survival when infecting a permissive
host, the clam Ruditapes decussatus. Bulletin of Environmental Contamination and Toxicology
80: 512-515. IF=0.609.
89. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Growth performance and metabolic
utilization of diets with native and waxy maize starch by gilthead sea bream (Sparus aurata)
juveniles. Aquaculture 274: 101-108. IF=1.678.
90. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Hepatic glucokinase and glucose-6phosphatase responses to dietary carbohydrate sources in gilthead sea bream (Sparus aurata)
juveniles at different rearing temperatures. Comparative Biochemistry and Physiology - Part A
149: 80-86. IF=1.709.
91. Enes, P, Panserat, S, Kaushik, S, Oliva-Teles, A. 2008. Rearing temperature enhances hepatic
glucokinase but not glucose-6-phosphatase activities in European sea bass (Dicentrarchus labrax)
and gilthead sea bream (Sparus aurata) juveniles fed with the same levels of glucose.
Comparative Biochemistry and Physiology - Part A 150: 355-358. IF=1.709.
Phycology 43: 275-282. IF=1.826.
93. Erzini, K, Bentes, L, Coelho, R, Lino, PG, Monteiro, P, Ribeiro, J, Gonçalves, JMS. 2008. Catches in
"ghost-fishing" octopus and fish traps in the north-eastern Atlantic (Algarve, Portugal). Fishery
Bulletin (U.S.) 106: 321-327. IF=1.190.
94. Esteves, E, Andrade, JP. 2008. Diel and seasonal distribution patterns of eggs, embryos and larvae
of Twaite shad Alosa fallax fallax (Lacépède, 1803) in a lowland tidal river. Acta Oecologica 34:
172-185. IF=1.456.
95. Faubel, D, Lopes-Lima, M, Freitas, S, Pereira, L, Andrade, J, Checa, A, Frank, H, Matsuda, T,
2+
Machado, J. 2008. Effects of Cd on the calcium metabolism and shell mineralization of bivalve
Anodonta cygnea. Marine and Freshwater Behaviour and Physiology 41(2): 131-146. IF=1.271.
96. Fernandes, C, Fontaínhas-Fernandes, A, Cabral, D, Salgado, MA. 2008. Heavy metals in water,
sediment and tissues of Liza saliens from Esmoriz-Paramos lagoon, Portugal. Environmental
Monitoring and Assessment 136(1-3): 267-275. IF=1.035.
97. Fernandes, C, Fontaínhas-Fernandes, A, Ferreira, M, Salgado, MA. 2008. Oxidative stress
Archives of Environmental Contamination and Toxicology 55(2): 262-269. IF=1.864.
Environmental Monitoring and Assessment 145(1-3): 315-322. IF=1.035.
99. Ferraresso, S, Vitulo, N, Mininni, AN, Romualdi, C, Cardazzo, B, Negrisolo, E, Reinhardt, R,
Canario, AVM, Patarnello, T, Bargelloni, L. 2008. Development and validation of a gene
expression oligo microarray for the gilthead sea bream (Sparus aurata). BMC Genomics 9: 580.
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100. Ferreira, M, Antunes, P, Costa, J, Amado, J, Gil, O, Pousão-Ferreira, P, Vale, C, Reis-Henriques,
MA. 2008. Organochlorine bioaccumulation and biomarkers levels in culture and wild white
seabream (Diplodus sargus). Chemosphere 73: 1669-1674. IF=3.054.
101. Ferreira, M, Caetano, M, Costa, J, Pousão-Ferreira, P, Vale, C, Reis-Henriques, MA. 2008. Metal
accumulation and oxidative stress responses in, cultured and wild, white seabream from
Northest Atlantic. Science Total Environment 407: 638-646. IF=2.579.
102. Figueroa, F, Bueno, A, Korbee, N, Santos, R, Mata, L, Schuenhoff, A. 2008. Accumulation of
mycosporine-like amino acids in Asparagopsis armata grown in tanks with fishpond effluents of
gilthead sea bream, Sparus aurata. Journal of the World Aquaculture Society 39: 692-699.
IF=0.693.
103. Fonseca, PJ, Serrao, EA, Pina-Martins, F, Silva, P, Mira, S, Quartau, JA, Paulo, OS, Cancela, L. 2008.
The evolution of cicada songs contrasted with the relationships inferred from mitochondrial DNA
(Insecta, Hemiptera). Bioacoustics - The International Journal of Animal Sound and its Recording
18: 17-34. IF=0.722.
104. Francisco, SM, Congiu, L, Stefanni, S, Castilho, R, Brito, A, Ivanova, PP, Levy, A, Cabral, A, Kilias, G,
Almada, VC. 2008. Phylogenetic relationships of the North-eastern Atlantic and Mediterranean
forms of Atherina (Pisces, Atherinidae). Molecular Phylogenetics and Evolution 48: 782-788.
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105. Frasco, MF, Carvalho, F, Guilhermino, L, Stojan, J, Fournier, D. 2008. Cholinesterase enzymes:
Appraisal of inhibitory mechanisms for their use as an environmental biomarker of toxicity.
Marine Environmental Research 66: 42-43. IF=2.032.
106. Frasco, MF, Fournier, D, Carvalho, F, Guilhermino, L. 2008. Does mercury interact with the
inhibitory effect of dichlorvos on Palaemon serratus (Crustacea: Decapoda) cholinesterase?.
Science of the Total Environment 404: 88-93. IF=2.579.
107. Fraussen, K, Afonso, CML. 2008. A new Euthria (Gastropoda: Buccinidae) from the Cape Verde
Archipelago. Gloria Maris 47: 53-60.
108. Frija, LMT, Khmelinskii, IV, Serpa, C, Reva, ID, Fausto, R, Cristiano, MLS. 2008. Photochemistry of
5-allyloxy-tetrazoles: steady-state and laser flash photolysis study. Organic & Biomolecular
Chemistry 6: 1046-1055. IF=3.550.
109. Galay-Burgos, M, Power, DM, Llewellyn, L, Sweeney, GE. 2008. Thyroid hormone receptor
expression during metamorphosis of Atlantic halibut (Hippoglossus hippoglossus). Molecular and
Cellular Endocrinology 281: 56-63. IF=3.611.
110. Garcia-Santos, S, Fontainhas-Fernandes, A, Mancera, J, Wilson, JM. 2008. Metabolic and
osmoregulatory alterations and cell proliferation in gilthead sea bream (Sparus aurata) exposed
to cadmium. Comparative Biochemistry and Physiology Part A 150(3): S105. IF=1.709.
111. Garrido, S, Ben-Hamadou, R, Oliveira, PB, Cunha, ME, Chícharo, MA, van der Lingen, CD. 2008.
Diet and feeding intensity of sardine Sardina pilchardus: correlation with satellite-derived
chlorophyll data. Marine Ecology Progress Series 354: 245-256. IF=2.631.
112. Garrido, S, Rosa, R, Ben-Hamadou, R, Cunha, ME, Chícharo, MA, van der Lingen, CD. 2008. Spatiotemporal variability in fatty acid trophic biomarkers in stomach contents and muscle of Iberian
sardine (Sardina pilchardus) and its relationship with spawning. Marine Biology 154: 1053-1065.
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113. Goncalves, AF, Damasceno-Oliveira, A, Aluru, N, Vijayan, MM, Coimbra, J, Wilson, JM. 2008.
There is a cellular stress in response to hydrostatic pressure (3 MPa) acclimation in the shallow
water teleost Oncorhynchus mykiss. Comparative Biochemistry and Physiology Part A 150(3):
S107. IF=1.709.
114. Gonçalves, D, Teles, M, Alpedrinha, J, Oliveira, RF. 2008. Brain and gonadal aromatase activity
ans steroid hormone levels in female and polymorphic males of the peacock blenny Salaria pavo.
Hormones and Behavior 54: 717-725. IF=3.876.
115. Gonçalves, JMS, Bentes, L, Coelho, R, Monteiro, P, Ribeiro, J, Correia, C, Lino, PG, Erzini, K. 2008.
Non-commercial invertebrate discards in an experimental trammel net fishery. Fisheries
Management and Ecology 15: 199-210. IF=1.404.
116. Gonçalves, JMS, Bentes, L, Monteiro, P, Coelho, R, Corado, M, Erzini, K. 2008. Reducing discards
in a demersal purse-seine fishery. Aquatic Living Resources 21: 135-144. IF=0.667.
117. Gonçalves, OM, Fontaínhas-Fernandes, A, Castro, LC, Smolka, A, Wilson, J. 2008. Gastric proton
pump expression in teleost fishes: nongastric sites of expression in gastric versus agastric fishes.
Comparative Biochemistry and Physiology Part A 150(3): S106. IF=1.709.
118. Gonçalves, R, Scholze, M, Ferreira, AM, Martins, M, Correia, AD. 2008. The joint effect of
polycyclic aromatic hydrocarbons on fish behavior. Environmental Research 108: 205-213.
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119. Granja, HM, De Groot, TA, Costa, AL. 2008. Evidence for Pleistocene wet aeolian dune and
interdune accumulation, S. Pedro de Maceda, NW Portugal. Sedimentology 55(5): 1203-1226.
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120. Hamidov, A, Khaydarova, V, Sharipova, S, Costa, MC, Beltrão, J. 2008. Environmentally useful
technique - Portulaca oleracea Golden Purslane as a salt removal species. WSEAS Transactions on
Environment and Development 7(3): 117-122.
121. Haond, C, Canario, AVM. 2008. Re-examination of the immunolocalization of the cystic fibrosis
tansmembrane regulator in mouse kidney: evidence for its basolateral location in renal epithelial
cells. Nephron Physiology 110: 29. IF=1.937.
122. Hazin, H, Erzini, K. 2008. Assessing swordfish distribution in the South Atlantic from spatial
predictions. Fisheries Research 90: 45-55. IF=1.434.
123. Hermida, M, Saraiva, A, Cruz, C. 2008. Metazoan parasite community of a european eel (Anguilla
anguilla) population from an estuary in Portugal. Bulletin of the European Association of Fish
Pathologists 28(1): 35-40. IF=0.430.
124. Hernández, I, Pérez-Pastor, A, Mateio, JJ, Megina, C, Vergara, JJ. 2008. Growth dynamics of Ulva
rotundata (Chlorophyta) in a fish farm: implications for biomitigation at a large scale. Journal of
Phycology 44: 1080-1089. IF=2.811.
125. Hildahl, J, Power, DM, Björnsson, BT, Einarsdóttir, IE. 2008. Involvement of growth hormoneinsulin-like growth factor I system in cranial remodeling during halibut metamorphosis as
indicated by tissue- and stage-specific receptor gene expression and the presence of growth
hormone receptor protein. Cell and Tissue Research 332: 211-225. IF=2.740.
126. Hirschenhauser, K, Canario, AVM, Ros, AFH, Taborsky, M, Oliveira, RF. 2008. Social context may
affect urinary excretion of 11-ketotestosterone in African cichlids. Behaviour 145: 1367-1388.
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127. Huertas, M, Canario, AVM, Hubbard, PC. 2008. Changes in olfactory sensitivity during the
European Eel (Anguilla Anguilla) life cycle. Chemical Senses 33: S150. IF=3.041.
128. Huertas, M, Canario, AMV, Hubbard, PC. 2008. Chemical communication in the Genus Anguilla: a
miniriview. Behaviour 145: 1389-1407. IF=1.556.
129. Joaquim, S, Gaspar, MB, Matias, D, Ben-Hamadou, R, Arnold, WS. 2008. Rebiulding viable
spawner patches of the overfished Spisula solida (Mollusca: Bivalvia): a preliminary contribution
to fishery sustainability. ICES Journal of Marine Science 65: 60-64. IF=1.661.
(Teloestei, Salmonidae). Microscopy Research & Technique 71: 822-830. IF=1.883.
131. Kaczor, A, Almeida, R, Gomez-Zavaglia, A, Cristiano, MDS, Fausto, R. 2008. Molecular structure
and infrared spectra of the monomeric 3-(methoxy)-1,2-benzisothiazole 1,1-dioxide (methyl
pseudosaccharyl ether). Journal of Molecular Structure 876: 77-85. IF=1.594.
132. Kaczor, A, Proniewicz, LM, Almeida, R, Gomez-Zavaglia, A, Cristiano, MLS, Beja, AMM, Silva, MR,
Fausto, R. 2008. The Chapman-type rearrangement in pseudosaccharins: The case of 3(methoxy)-1,2-benzisothiazole 1,1-dioxide. Journal of Molecular Structure 892: 343-352.
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133. Kadar, E, Checa, AG, Damasceno-Oliveira, A, Machado, JP. 2008. Shell nacre ultrastructure and
depressurisation dissolution in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus.
Journal of Comparative Physiology B 178(1): 123-130. IF=1.698.
134. Kadar, E, Davis, SA, Lobo-da-Cunha, A. 2008. Cytoenzymatic investigation of intracellular digestion in
the symbiont-bearing hydrothermal bivalve Bathymodiolus azoricus. Marine Biology 153: 995-1004.
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135. Kijjoa, A, Gonzalez, MJ, Pinto, MM, Nascimento, MSJ, Campos, N, Mondranondra, I-O, Silva, AMS,
Eaton, G, Herz, W. 2008. Cytotoxicity of prenylated xanthones and other constituents from the
wood of Garcinia merguensis. Planta Medica 74(8): 864-866. IF=1.960.
136. Kolmakov, NN, Kube, M, Reinhardt, R, Canario, AV. 2008. Analysis of the goldfish Carassius
auratus olfactory epithelium transcriptome reveals the presence of numerous non-olfactory
GPCR and putative receptors for progestin pheromones. BMC Genomics 9: 429. IF=3.926.
137. Kortner, TM, Rocha, E, Silva, P, Jørstad, TS, Castro, LFC, Arukwe, A. 2008. Genomic approach in
evaluating the role of androgens on the growth of Atlantic cod (Gadus morhua) previtellogenic
oocytes. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 3: 205-218.
IF=1.543.
138. Kovitvadhi, S, Kovitvadhi, U, Sawangwong, P, Machado, J. 2008. A laboratory-scale recirculating
aquaculture system for juveniles of freshwater pearl mussel Hyriopsis (Limnoscapha) myersiana
(Lea, 1856). Aquaculture 275: 169-177. IF=1.678.
139. Ladah, L, Feddersen, F, Serrao, EA, Pearson, GA. 2008. Egg release and settlement patterns of a
dioecious and hermaphrodite fucoid algae during the tidal cycle. Marine Biology 155(6): 583-591.
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140. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2008. Fish assemblages and rapid colonisation after
enlargement of an artificial reef off the Algarve (Southern Portugal) coast. Marine Ecology 28:
435-448. IF=1.953.
141. Leitão, F, Santos, MN, Erzini, K, Monteiro, CC. 2008. The effect of predation on artificial reef
juvenile demersal fish species. Marine Biology 153: 1233-1244. IF=1.953.
142. Leite, RB, Brito, AB, Cancela, ML. 2008. An oxygen molecular sensor, the HIF prolyl-4 hydroxylase,
in the marine protist Perkinsus olseni. Protist 159: 355-368. IF=3.923.
143. Leliaert, F, Malta, E-J, Engelen, AH, Mineur, F, De Clerck, O. 2008. Quindao algal bloom culprit
identified. Marine Pollution Bulletin 56: 1516. IF=2.562.
144. Lima, D, Santos, MM, Ferreira, AM, Micaelo, C, Reis-Henriques, MA. 2008. The use of the shanny
Lipophrys pholis for pollution monitoring: A new sentinel species for the northwestern European
marine ecosystems. Environment International 34(1): 94-101. IF=3.516.
145. Lima, I, Peck, MR, Rendon-Von Osten, J, Soares, AMVM, Guilhermino, L, Rotchell, J. 2008. Ras
gene in marine mussels: a molecular level response to petrochemical exposure. Marine Pollution
Bulletin 56: 633-640. IF=2.562.
146. Lobo-da-Cunha, A, Calado, G. 2008. Histology and ultrastructure of the salivary glands in Bulla striata
(Mollusca, Opisthobranchia). Invertebrate Biology 127: 33-44. IF=1.217.
147. Lopes-Lima, M, Bleher, R, Forg, T, Hafner, M, Machado, J. 2008. Studies on a PMCA-like protein in
the outer mantle epithelium of Anodonta cygnea: insights on calcium transcellular dynamics.
Journal of Comparative Physiology B - Biochemical Systemic and Environmental Physiology
178(1): 17-25. IF=1.698.
148. Luís, A, Rocha, E, Oliveira, E, Silva, J, Barros, A, Sousa, M. 2008. Stereological analysis of
mitochondria and smooth endoplasmic reticulum distribution in oocytes in prophase I.
Microscopy and Microanalysis 14(S3): 103-104. IF=2.992.
149. Luo, SJ, Johnson, WE, Martenson, J, Antunes, A, Martelli, P, Uphyrkina, O, Traylor-Holzer, K,
Smith, JLD, O’ rien, SJ. 2008. Subspecies genetic assignments of worldwide captive tigers
increase conservation value of captive populations. Current Biology 18: 592-596. IF=10.777.
150. Luttikhuizen, PC, Campos, J, Bleijswijk, JV, Peijnenburg, KTCA, van der Veer, H-W. 2008.
Phylogeography of the commom shrimp, Crangon crangon (L.) across its distribution range.
Molecular Phylogenetics and Evolution 46: 1015-1030. IF=3.871.
151. Magalhães, C, Bano, N, Wiebe, W, Bordalo, AA, Hollibaugh, J. 2008. Dynamics of nitrous oxide
reductase genes (nosZ) in intertidal rocky biofilms and sediments of the Douro River estuary
(Portugal), and their relation to N-biogeochemistry. Microbial Ecology 55: 259-269. IF=2.885.
152. Magalhães, C, Teixeira, C, Teixeira, R, Machado, A, Azevedo, I, Bordalo, AA. 2008. Dissolved
organic carbon and nitrogen dynamics in the Douro River estuary, Portugal. Ciencias Marinas 34:
271-282. IF=1.038.
153. Maggs, CA, Castilho, R, Foltz, D, Henzler, C, Jolly, T, Kelly, J, Olsen, J, Perez, KE, Stam, W, Vainola,
R, Viard, F, Wares, J. 2008. Evaluating signatures of glacial refugia for North Atlantic Marine
Organims. Ecology 89(sp11): S108-S122. IF=4.874.
154. Makridis, P, Martins, S, Reis, J, Dinis, MT. 2008. Use of probiotic bacteria in the rearing of
Senegalese sole (Solea senegalensis) larvae. Aquaculture Research 39: 627-634. IF=0.991.
155. Marques, A, Antunes, A, Fernandes, PA, Ramos, MJ. 2008. Computational optimisation of
AG18051 inhibitor for amyloid-beta binding alcohol dehydrogenase (ABAD) enzyme.
International Journal of Quantum Chemistry 108: 1982-1991. IF=1.317.
156. Martinez-Pastor, F, Cabrita, E, Soares, F, Anel, L, Dinis, MT. 2008. Multivariate cluster analysis to
study motility activation of Solea senegalensis spermatozoa: a model for marine teleosts.
Reproduction 135: 449-459. IF=3.073.
157. Martins da Costa, P, Bica, A, Vaz-Pires, P, Bernardo, F. 2008. Effects of antimicrobial treatment on
selection of resistant Escherichia coli in broiler fecal flora. Microbial Drug Resistance Mechanisms, Epidemiology and Disease 14(4): 266-306. IF=1.800.
158. Martins da Costa, P, Vaz-Pires, P, Bernardo, F. 2008. Antimicrobial resistance in Escherichia coli
isolated in wastewater and sludge from poultry slaughterhouse wastewater plants. Journal of
Environmental Health 70: 40-45. IF=0.805.
159. Martins, R, Ramos, M, Herfindal, L, Sousa, JA, Doskeland, S, Vasconcelos, VM. 2008. Antimicrobial
and cytotoxic assessment of marine cyanobacteria extracts. Marine Drugs 6(1): 1-11. IF=1.200.
160. Masterson, P, Arenas, FA, Thompson, RC, Jenkins, SR. 2008. Interaction of top down and bottom
up factors in intertidal rockpools: Effects on early successional macroalgal community
composition, abundance and productivity. Journal of Experimental Marine Biology and Ecology
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161. Mendonça, VM, Raffaelli, DG, Boyle, P, Hoskins, S. 2008. Spatial and temporal characteristics of
benthic invertebrate communities at Culbin Sands lagoon, Moray Firth, NE Scotland, and impacts
of the disturbance of cockle harvesting. Scientia Marina 72: 265-278. IF=1.075.
162. Mendoza, H, Freijanes, K, Carmona, L, Ramos, A, Duarte, V, Varela, J, de la Jara, A. 2008.
Characterization of Dunaliella salina strains by flow cytometry: a new technique to select carotene hyperproducing strains. Electronic Journal of Biotechnology 11(4): 1-13. IF=0.920.
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164. Mitchell, NC, Beier, C, Rosin, PL, Quartau, RQ, Tempera, F. 2008. Lava penetrating water:
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165. Mohseni, M, Ozorio, ROA, Pourkazemi, M, Bai, SC. 2008. Effects of dietary L-Carnitine
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166. Monteiro, J, Borges, R, Robalo, J, Almada, VC, Henriques, S, Gonçalves, EJ. 2008. Larval
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167. Monteiro, P, Ribeiro, D, Silva, JA, Bispo, J, Gonçalves, JMS. 2008. Ichthyofauna assemblages from
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168. Monteiro, SM, Rocha, E, Fontaínhas-Fernandes, A, Sousa, M. 2008. Quantitative histopathology of
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169. Monteiro, SM, Rocha, E, Mancera, JM, Fontaínhas-Fernandes, A, Sousa, M. 2008. A stereological
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170. Morais, J, Augusto, M, Carvalho, AP, Vale, M, Vasconcelos, VM. 2008. Cyanobacteria
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171. Morais, P. 2008. Review on the major ecosystem impacts caused by damming and watershed
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172. Moreira, IS, Peres, H, Couto, A, Eenes, P, Oliva-Teles, A. 2008. Temperature and dietary
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173. Moreira-Silva, J, Coimbra, J, Wilson, J. 2008. Weatherloach (Misgurnus anguillicaudatus) actively
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174. Moreira-Silva, J, Coimbra, J, Steffensen, J, Wilson, J. 2008. Effect of ammonia on weatherloach
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175. Morgado, I, Melo, EP, Lundberg, E, Estrela, NL, Sauer-Eriksson, E, Power, DM. 2008. Hormone
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Cellular Endocrinology 295: 48-58. IF=3.611.
176. Moschino, V, Marin, MG, Chícharo, L. 2008. Effects of hydraulic dredging on the physiological
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178. Muiños, SB, Frank, M, Maden, C, Hein, JR, van der Flierdt, T, Lebreiro, SM, Gaspar, L, Monteiro,
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179. Nesshöver, C, Müssner, R, Henle, K, Sousa-Pinto, I. 2008. Linking biodiversity research and policy
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180. Nicastro, KR, Zardi, GI, McQuaid, CD. 2008. Movement behaviour and mortality in invasive and
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181. Nicastro, KR, Zardi, GI, McQuaid, CD, Teske, PR, Barker, NP. 2008. Coastal topography drives
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182. Nunes, B, Cardoso, MF, Carvalho, F, Guilhermino, L. 2008. The microalga Tetraselmis chuii
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183. Nunes, B, Gaio, AR, Carvalho, F, Guilhermino, L. 2008. Behaviour and biomarkers of oxidative
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184. Nunes, MA, Santos, S, Cordeiro, JM, Neves, P, Silva, VS, Sykes, AV, Morgado, F, Dunant, Y,
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185. Nuzzo, M, Hornibrook, ERC, Hensen, C, Parkes, RJ, Cragg, BA, Rinna, J, von Deimling, JS, Sommer,
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186. Oliva Teles, L, Pereira, E, Saker, M, Vasconcelos, V. 2008. The use of virtual models for predicting
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187. Oliveira, JC, Soares, F, Engrola, S, Dopazo, CP, Bandín, I. 2008. Antemortem versus postmortem
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188. Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2008. Uptake and depuration of anatoxin-a by the
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189. Ozório, ROA. 2008. Swimming activity and Non-Protein Energy (NPE) metabolism in fish. Current
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190. Pacheco, AMG, Freitas, MC, Baptista, MS, Vasconcelos, MTSD, Cabral, JP. 2008. Elemental levels
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191. Pacheco, AMG, Freitas, MC, Baptista, MS, Vasconcelos, MTSD, Cabral, JP. 2008. Joint assessment
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techniques. Journal of Radioanalytical and Nuclear Chemistry 276(1): 135-141. IF=0.659.
192. Paiva, VH, Ramos, JA, Martins, J, Almeida, A, Carvalho, A. 2008. Foraging habitat selection by
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193. Palma, J, Bureau, D, Andrade, JP. 2008. Effects of binder type and binder addition on the growth
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194. Palma, J, Correia, M, Andrade, JP. 2008. Usefulness of flat bottom tanks on the settlement of
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195. Palma, J, Stockdale, J, Correia, M, Andrade, J. 2008. Growth and survival of adult long snout
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196. Páscoa, I, Fontaínhas-Fernandes, A, Wilson, J. 2008. Ammonia tolerance in the zebrafish (Danio
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197. Penna, N, Bos, M, Baker, T, Scherneck, H-G. 2008. Assessing the accuracy of predicted ocean tide
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198. Pereira, E, OlivaTeles, F, Vasconcelos, V. 2008. Variation of environmental parameters and
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199. Pereira, R, Kraemer, G, Yarish, C, Sousa-Pinto, I. 2008. Nitrogen uptake by gametophytes of
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200. Peres, H, Oliva-Teles, A. 2008. Lysine requirement and efficiency of lysine utilization in turbot
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201. Perez-Cerezales, S, Martinez-Paramo, S, Beirão, J, Cabrita, E, Herraez, MP. 2008. Analysis of
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202. Power, DM, Einarsdóttir, IE, Pittman, K, Sweeney, GE, Hildahl, J, Campinho, MA, Silva, N, Sæle, O,
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203. Quintaneiro, C, Querido, D, Monteiro, M, Guilhermino, L, Morgado, F, Soares, AMVM. 2008.
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204. Ramos, A, Bandarra, NM, Rema, P, Vaz-Pires, P, Nunes, ML, Andrade, AM, Cordeiro, AR, Valente,
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205. Ramos, A, Coesel, S, Marques, A, Rodrigues, M, Baumgartner, A, Noronha, J, Rauter, A, Brenig, B,
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206. Ramos, A, Varela, J. 2008. Biochemistry and molecular biology in Portugal: an overview of past
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207. Range, P, Chapman, MG, Underwood, AJ. 2008. Field experiments with "cageless" methods to
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208. Reis, PA, Almeida, CMR. 2008. Matrix importance in animal material pre-treatment for metal
determination. Food Chemistry 107(3): 1294-1299. IF=2.696.
209. Reis, PA, Valente, LMP, Almeida, CMR. 2008. A fast and simple methodology for determination of
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210. Reis-Santos, PN, McCormick, SD, Wilson, JM. 2008 Ionoregulatory changes during
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211. Rema, P, Conceição, LEC, Evers, F, Castro-Cunha, M, Dinis, MT, Dias, J. 2008. Optimal dietary
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213. Ribeiro, L, Couto, A, Olmedo, M, Alvarez-Blazquez, B, Linares, F, Valente, LMP. 2008. Digestive
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214. Rocha, RJ, Ribeiro, L, Costa, R, Dinis, MT. 2008. Does the presence of microalgae influence fish
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215. Rozenfeld, AF, Arnaud-Haond, S, Hernández-García, E, Eguíluz, VM, Serrao, EA, Duarte, CM. 2008.
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216. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A. 2008. Dietary protein requirement of white sea bream
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217. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A. 2008. Dietary lipid utilisation by white sea bream
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218. Sá, R, Pousão-Ferreira, P, Oliva-Teles, A. 2008. Effect of dietary starch source (normal vs. waxy)
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219. Saavedra, M, Conceição, LEC, Helland, S, Pousão-Ferreira, P, Dinis, MT. 2008. Effect of lysine and
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220. Saavedra, M, Conceição, LEC, Pousão-Ferreira, P, Dinis, MT. 2008. Metabolism of tryptophan,
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221. Salgueiro, E, Voelker, A, Abrantes, F, Meggers, H, Pflaumann, U, Loncaric, N, González-Álvarez, R,
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222. Sánchez Goñi, MF, Landais, A, Fletcher, W, Naughton, F, Desprat, S, Duprat, J. 2008. Contrasting
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223. Sandra, J, Gaspar, MB, Matias, D, Ben-Hamadou, R, Arnold, WS. 2008. Rebuilding viable spawner
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225. Saqrane, S, El Ghazali, I, Pudra, B, Bouarab, L, Vasconcelos, V. 2008. Effects of cyanobacteria
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226. Saraiva, A, Hermida, M, Costa, MJ, Maia, C, Reis, AR, Cruz, C, Valente, A. 2008. First record of
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227. Saverwyns, H, Visser, A, Van Durme, J, Power, D, Morgado, I, Kennedy, MW, Knox, DP,
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228. Sawangwong, P, Wattanadilok, R, Kijjoa, A, Silva, AMS, Eaton, G, Herz, W. 2008. Secondary
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229. Schmidt, PS, Serrao, EA, Pearson, GA, Riginos, C, Rawson, PD, Hibish, TJ, Brawley, SH, Trussell, GC,
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230. Scholz, T, Boane, C, Saraiva, A. 2008. New metacestodes of gryporhynchid tapeworms (Cestoda:
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231. Scott, AP, Hirschenhauser, K, Bender, N, Oliveira, R, Earley, RL, Sebire, M, Ellis, T, Pavlidis, M,
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232. Seixas, P, Rey-Méndez, M, Valente, LMP, Otero, A. 2008. Producing juvenile Artemia as preys for
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233. Serrano, RM, Barata, EN, Birkett, MA, Hubbard, PC, Guerreiro, PS, Canario, AVM. 2008.
Behavioral and olfactory responses of female Salaria pavo (Pisces: Blenniidae) to a putative
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234. Serrano, RM, Lopes, O, Hubbard, PC, Araújo, J, Canario, AVM. 2008. Seasonal cell differentiation
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236. Silva, J, Feijóo, P, Santos, R. 2008. Underwater measurements of carbon dioxide evolution in
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237. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2008. Can the limited marsupium space be a
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239. Simes, DC, Viegas, CSB, Williamson, MK, Price, PA, Cancela, ML. 2008. Purification of matrix Gla
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240. Soares, SS, Henao, F, Aureliano, M, Gutiérrez-Merino, C. 2008. Vanadate induces necrotic cell
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241. Soares, SS, Martins, H, Gutiérrez-Merino, C, Aureliano, M. 2008. Vanadium and cadmium in vivo
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242. Solé, M, Lima, D, Reis-Henriques, MA, Santos, MM. 2008. Stress biomarkers in juvenile Senegal
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243. Solé, M, Lobera, G, Lima, D, Reis-Henriques, MA, Santos, MM. 2008. Estearases activities and
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244. Sousa, R, Antunes, C, Guilhermino, L. 2008. Ecology of the invasive Asian clam Corbicula fluminea
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245. Sousa, R, Dias, S, Freitas, V, Antunes, C. 2008. Subtidal macrozoobenthic assemblages along the
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248. Sousa, R, Nogueira, AJA, Antunes, C, Guilhermino, L. 2008. Growth and production of Pisidium
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249. Sousa, R, Nogueira, AJA, Gaspar, MB, Antunes, C, Guilhermino, L. 2008. Growth and extremely
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251. Stehmann, MFW, Seret, B, Costa, EM, Baro, J. 2008. Neoraja iberica n. sp., a new species of
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252. Stoichev, T, Baptista, MS, Basto, MCP, Carvalho, PN, Vasconcelos, MTSD. 2008. Application of
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Analytical and Bioanalytical Chemistry 391(1): 425-432. IF=3.328.
253. Tanguy, A, Bierne, N, Saavedra, C, Pina, B, Bachère, E, Kube, M, Bazin, E, Bonhomme, F, Boudry,
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254. Tavares, PC, Machado, M, Cancela da Fonseca, L. 2008. Colonization process in soft-bottom
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255. Thomas, PJ, Murray, AS, Granja, HM, Jain, M. 2008. Optical dating of Late Quaternary coastal
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259. Troyer, JL, Vandewoude, S, Slattery, J, Mcintosh, C, Franklin, S, Antunes, A, Johnson, WE, O’ rien,
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260. Tsui, TKN, Hung, CCY, Nawata, MC, Wilson, JM, Wright, PA, Wood, CM. 2008. In vitro evidence
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261. Vasconcelos, MTSD, Leal, MFC. 2008. Exudates of different marine algae promote growth and
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262. Vasconcelos, P, Carvalho, S, Castro, M, Gaspar, MB. 2008. The artisanal fishery for muricid
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4: 392-399.
264. Vasconcelos, P, Lopes, B, Castro, M, Gaspar, MB. 2008. Gametogenic cycle of Hexaplex
(Trunculariopsis) trunculus (Gastropoda: Muricidae) in the Ria Formosa lagoon (Algarve coast,
southern Portugal). Journal of the Marine Biological Association of the United Kingdom 88: 321329. IF=1.056.
265. Vasconcelos, VM, Almeida, L. 2008. Allelopathic effects of freshwater cyanobacteria species on
the green alga Ankistrodesmus falcatus. Fresenius Environmental Bulletin 17(9a): 1264-1269.
IF=0.463.
266. Vaselli, S, Bertocci, I, Maggi, E, Benedetti-Cecchi, L. 2008. Effects of mean intensity and temporal
variance of sediment scouring events on assemblages of rocky shore. Marine Ecology Progress
Series 364: 57-66. IF=2.631.
267. Vaselli, S, Bulleri, F, Benedetti-Cecchi, L. 2008. Hard coastal-defence structures as habitats for
native and exotic rocky-bottom species. Marine Environmental Research 66(4): 395-403.
IF=2.032.
268. Vaz-Pires, P, Seixas, P, Mota, M, Lapa-Guimarães, J, Pickova, J, Lindo, A, Silva, T. 2008. Sensory,
microbiological, physical and chemical properties of cuttlefish (Sepia officinalis) and broadtail
shortfin squid (Illex coindetii) stored in ice. LWT (Lebensmittel-Wissenschaft und -Technologie) Food Science and Technology 41(9): 1655-1664. IF=2.114.
269. Veríssimo, E, Berry, N, Gibbons, P, Cristiano, MLS, Rosenthal, PJ, Gut, J, Ward, SA, O'Neill, PM.
2008. Design and synthesis of novel 2-pyridone peptidomimetic falcipain 2/3 inhibitors.
Bioorganic & Medicinal Chemistry Letters 18: 4210-4214. IF=2.531.
270. Viegas, CS, Simes, DC, Laizé, V, Williamson, MK, Price, PA, Cancela, ML. 2008. Gla-rich protein
(GRP), a new vitamin K-dependent protein identified from sturgeon cartilage and highly
conserved in vertebrates. Journal of Biological Chemistry 283: 36655-36664. IF=5.520.
271. Vieira, LR, Sousa, A, Frasco, MF, Lima, I, Morgado, F, Guilhermino, L. 2008. Acute effects of
benzo[a]pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus
microps (Teleostei, Gobiidae). Science of the Total Environment 395: 87-100. IF=2.579.
272. Viejo, RM, Arenas, F, Fern ndez, C,
mez, M. 2008. Mechanisms of succession along the
emersion gradient in intertidal rocky shore assemblages. Oikos 117(3): 376-389. IF=2.970.
273. Volckaert, FAMJ, Barbier, M, Canario, AVM, Olsen, JL, Wesnigk, J. 2008. Empowering marine
science through genomics. Marine Genomics 1: 33-35.
274. Wirtz, P. 2008. The Gulf of Guinea goby-shrimp-symbiosis and a review of goby - thalassinidean
associations. Arquipelago, Life and Marine Sciences 25: 71-76.
275. Wirtz, P. 2008. New records of the giant cilate Zoothamnium niveum (Protozoa, Peritrichia).
Arquipelago, Life and Marine Sciences 25: 89-91.
276. Wirtz, P, d'Udekem d'Acoz, C. 2008. Crustaceans associated with Cnidaria, Bivalvia, Echinoidea
and Pisces at São Tomé and Príncipe Islands (eastern central Atlantic). Arquipelago, Life and
Marine Sciences 25: 63-69.
277. Zardi, GI, McQuaid, CD, Nicastro, KR, Erlandsson, J. 2008. Sand and wave induced mortality in
invasive (Mytilus galloprovincialis) and indigenous (Perna perna) mussels. Marine Biology 153:
853-858.
(*) Protocol of collaboration with LNEG.
(**) Protocol of collaboration with University of Minho.
PUBLICATIONS IN INTERNATIONAL PEER REVIEWED PROCEEDINGS
1.
Afonso, A. 2008. Review of aquaculture development in Portugal and Europe. Proceedings of the
International Workshop: Developing a Sustainable Aquaculture Industry in the Azores, 2-5 June,
Horta, Azores, Portugal. Arquipélago, Life and Marine Sciences, Supplement 7: 6-7.
2.
Antunes, P, Gil, O, Hendriks, AJ, Huijbregts, MAJ, Reis-Henriques, MA. 2008. Comparing
elimination pattern of PCBs in juvenile and adult seabass (Dicentrarchus labrax). DIOXIN 2008 28th. International Symposium on Halogenated Environmental Organic Pollutants (POPs), 17-22
August, Birmingham, England, UK. Organohalogen Compounds 70: 1989-1992.
3.
Antunes, P, Gil, O, Reis-Henriques, MA. 2008. Accumulation of PCB metabolites in plasma of
seabass (Dicentrarchus labrax). DIOXIN 2008 - 28th. International Symposium on Halogenated
Environmental Organic Pollutants (POPs), 17-22 August, Birmingham, England UK.
Organohalogen Compounds 70: 1985-1988.
4.
Gonçalves, JFM, Damasceno-Oliveira, AP. 2008. Aquaculture project formulation. Proceedings of
the International Workshop: Developing a Sustainable Aquaculture Industry in the Azores, 2-5
June, Horta, Azores, Portugal. Arquipélago - Life and Marine Sciences, Supplement 7: 25-29.
5.
Letão, A, Damasceno-Oliveira, A, Pereira, CM, Coimbra, JC, Wilson, JM. 2008. Transport stress in
glass eels. 6th AIEC Congress, Iberian Association of Comparative Endocrinology, 10-13
September, Cadiz, Spain. Avanços em Endocrinologia Comparativa. Muñoz-Cueto, JA, Mancera,
JM, Martínez-Rodríguez, G (Eds), Vol. 4, pp. 63-68. ISBN 978-84-9828-152-1.
6.
Martins, M, Santos, ES, Barros, RJ, Costa, MC. 2008. Treatment of acid mine drainage with
sulphate-reducing bacteria using a two-stage bioremediation process. Proceedings of the 10th
International Mine Water Association Congress - IMWA`08, 2-5 June, Carlsbad, Czech Republic,
pp. 297-300.
7.
Paiva, AP, Malik, P, Rosa, AM, Castro, C, Costa, MC. 2008. Extraction of metal ions from chloride
media: can malonamide derivatives be useful?. Proceedings of the International Solvent
Extraction Conference - ISEC 2008, 15-19 September, Tucson, Arizona, USA. Moyer, BA. (Ed), Vol.
2, pp. 1439-1444.
8.
Rangel, MO, Gonçalves, JMS, Almeida, C, Afonso, C, Costa, C, Erzini, K, Oliveira, F, Monteiro, P,
Ribeiro, J, Veiga, P. 2008. Underwater eco-tourism routes – a case study in Central Algarve.
Proceedings of the International Association for The Scientific Knowledge International
Conference, 26-28 May, Aveiro, Portugal. Costa, C, Cravo, P (Eds), pp. 25-32.
OTHER INTERNATIONAL PUBLICATIONS
1.
Anne, I, Fidalgo, ML, Søndergaard, M. 2008. Bacterioplankton dynamics in a series of wastewater
stabilization ponds (North of Portugal). Verhandlungen des Internationalen Verein Limnologie
30(3): 377-380.
2.
Casimiro, S, Fidalgo, ML. 2008. Lethal and behavioural responses of the freshwater shrimp
Atyaephyra desmarestii to chemical substances used in textile industry. Verhandlungen des
Internationalen Verein Limnologie 30(4): 541-545.
3.
Fidalgo, ML. 2008. A contribution to the incorporation of toxicity limitations in Portuguese
regulations relative to textile discharges - preliminary results. Verhandlungen des Internationalen
Verein Limnologie 30(4): 546-550.
4.
Stringuetti, C, Guilhermino, L, Da Silva, EM. 2008. Cholinesterase activity in the head of wild
Poecilia reticulate from Bahia, Brazil: biochemical characterization, effects of sample storage and
normal range of activity. Journal of the Brazilian Society of Ecotoxicology 3: 57-63.
OTHER NATIONAL PUBLICATIONS
1.
Araújo, NCP, O’Neill, PM, Cristiano, MLS. 2008. Design and Synthesis of Plasmodium DNADirected Endoperoxides. Revista Portuguesa de Farmácia L-II(3): 40.
2.
Baptista, P, Bastos, L, Cunha, T, Bernardes, C, Dias, J. 2008. Aplicação de metodologias de
monitorização GPS em litorais arenosos: geração de modelos de elevação do terreno. Revista de
Gestão Costeira Integrada - Revista n.º 8, fascículo 1 - Ano 7.
3.
Bringela, A, Almeida, R, Araújo, NCP, Veríssimo, E, O’Neill, PM, Cristiano, MLS. 2008. Synthesis of
New Peptidic Cysteine Protease Inhibitors. Revista Portuguesa de Farmácia L-II(3): 177.
4.
Cabral, JP. 2008. Gonçalo Sampaio e Baltasar Merino, e o estudo da Flora ibérica. Análise de
documentación epistolar. Murguía (Revista Galega de Historia) 15/16: 111-136.
5.
Cabral, JP. 2008. Gonçalo Sampaio na Póvoa de Varzim em 1920: cinco meses de trabalho
liquenológico excepcional. Boletim Cultural da Póvoa de Varzim 42: 384-431.
6.
Cabral, L, Araújo, NCP, O’Neill, PM, Cristiano, MLS. 2008. Synthesis of new DNA-directed prodrugs as potential antimalarial agents. Revista Portuguesa de Farmácia L-II(3): 178.
7.
Dias, J. 2008. Douradas e robalos vegetarianos: ficção ou realidade? UALGzine.
8.
Dinis, MT, Soares, F, Cabrita, E. 2008. REPROSOL - Optimização da reprodução em linguado (Solea
senegalensis). In: Investigação e Desenvolvimento no Sector das Pescas. Cadernos Promar nº1,
DGPA, pp. 71-74.
9.
Gonçalves, JMS, Veiga, P, Machado, D, Oliveira, F, Bentes, L, Monteiro, P, Coelho, R, Ruano, M,
Ribeiro, J, Erzini, K. 2008. Recrutamento de Espécies Piscícolas de Interesse Comercial no Estuário
do Rio Arade. In: Investigação e Desenvolvimento no Sector das Pescas. Cadernos Promar nº1,
DGPA, pp. 45-49.
10. Guilhermino, L. 2008. Biomarkers for the assessment of effects and/or exposure to
environmental contaminants: a special reference to pesticides. Proceedings of the Summer
School Pesticides – Environment 2007. Quaderno Grifa nº 26: 407-416. ISBN 978-88-95221-01-4.
11. Martins da Costa, P. 2008. Biosafety in food producing qnimals - The poultry production
Perspective. Revista da Ordem dos Médicos Veterinários Portugueses nº 49: 26-31.
12. Weber, M. 2008. O portinho da Aguda, a sua evolução e situação actual. Boletim da Associação
Cultural Amigos de Gaia 66, Vol. 11, pp. 64-68.
B.
ADVANCED TRAINING
PhD THESES
1.
Antunes, Paulo José. 2008. Accumulation of PCB congeners and DDT compounds in fish: influence
of size and reproductive cycle. PhD thesis. Supervisor: Odete Gil (INIAP/IPIMAR), Co-supervisor:
Maria Armanda Reis-Henriques.
2.
Ascenso, Rita. 2008. Study of Perkinsus olseni infection mechanisms: Identification and regulation
of parasite genes differentially expressed is response to host and environmental stress. PhD
thesis, University of Algarve. Co-supervisor: Maria Leonor Cancela, G Vssta.
3.
Azevedo, Maria Isabel. 2008. Ecological modelling of Douro Estuary: influence of river flow
variability on estuarine water quality and primary production. PhD thesis in Aquatic Sciences,
ICBAS, University of Porto. Supervisor: Adriano A Bordalo, Co-supervisor: Pedro Duarte
(University Fernando Pessoa).
4.
Baptista, Mafalda. 2008. Chemical factors related with freshwater blooms of cyanobacteria. PhD
thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto.
Supervisor: Maria Teresa SD Vasconcelos.
5.
Barbosa, Alexandra. 2008. A study of visual cues that elicit changeable camouflage in cuttlefish.
PhD thesis in Aquatic Sciences, ICBAS, University of Porto. In collaboration with the Marine
Biology Laboratory (MBL, USA). Supervisor: Roger Hanlon, Paulo Vaz-Pires, Gil Rosenthal.
6.
Boane, Custódio. 2008. Parasitas de carpa de Moçambique. PhD thesis, Faculty of Sciences,
University of Porto. Supervisor: Aurélia Saraiva.
7.
Coesel, Saccha Nicole. 2008. Regulation of the carotenoid biosynthetic pathway in the green
microalga Dunaliella salina and the diatom Phaeodactylum tricornutum. PhD thesis, University of
Algarve. Supervisor: João Varela and Chris Bowler.
8.
Delgado, Cláudia. 2008. Gonad development and hormone titres in loggerhead sea turtles
Caretta caretta in the NE Atlantic. PhD thesis in Marine Biology, University of Madeira.
9.
Enes, Paula Cristina. 2008. Utilization of dietary carbohydrates by European sea bass
(Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.) juveniles. PhD thesis in
Biology, Faculty of Sciences, University of Porto. Supervisor: Aires Oliva-Teles.
10. Engrola, Sofia. 2008. Improving growth performance of Senegalese sole postlarvae. PhD thesis,
University of Algarve. Supervisors: Maria Teresa Dinis and Luís Conceição.
11. Frija, Luís Miguel. 2008. Investigation of structural effects on the reactivity of heterocyclic
bioactive compounds. PhD thesis, University of Algarve. Supervisor: Nuna Araújo, in collaboration
with Paul M O'Neill (University of Liverpool).
12. Mata, Leonardo. 2008. Integrated aquaculture of Bonnemaisoniaceae algae: physiological and
nutritional controls of biomass production and of halogenated metabolite content. PhD thesis,
University of Algarve. Supervisor: Rui Santos.
13. Morais, Pedro Miguel. 2008. Engraulis encrasicolus sensus lato population dynamics in the
Guadiana estuary and adjacent coastal area. PhD thesis, University of Algarve. Supervisors: Maria
Alexandra Chicharo and Luís Chicharo.
14. Pereira, Elisa. 2008. Cianobactérias tóxicas em sistemas artificiais (albufeira do Torrão- Rio
Tâmega e na ETAR de Esmoriz). PhD thesis in Biology, Faculty of Sciences, University of Porto.
Supervisor: Vítor Vasconcelos.
15. Pinto, Carla Batista. 2008. Peroxissomas in brown trout (Salmo trutta fario): regulation by
estrogens. PhD thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisors: Alexandre
Lobo da Cunha and Eduardo Rocha, Pedro Rodrigues (IBMC / INEB LA, ICBAS).
16. Resende, Albina Dolores. 2008. Seasonal and toxicological study of brown trout (Salmo trutta)
kidney and liver peroxisomes. PhD thesis in Biomedical Sciences, ICBAS, University of Porto.
Supervisors: Alexandre Lobo da Cunha and Eduardo Rocha.
17. Ribeiro, Ricardo do Amaral. 2008. Utilização de efluentes industriais (farinha de sangue e soro de
leite) na elaboração de ração para pirarucu (Arapaima gigas, Curvier.). PhD thesis in
collaboration with CIIMAR (Portugal), INPA (Brazil) and University of Acre (Brazil). Supervisors:
Manuel Pereira (INPA), Rodrigo Ozório.
18. Rocha, Ana Maria. 2008. Clonação, caracterização funcional e expressão dos receptores das
gonadotrofinas (FSH e LH) no robalo europeu (Dicentrarchus labrax, L.). PhD thesis, CSIC,
University of Sevilha. Co-supervior: João Coimbra.
19. Saavedra, Margarida. 2008. Amino acid requirements of white seabream (Diplodus sargus)
larvae: effects on growth and performance. PhD thesis in collaboration with IPIMAR (Portugal).
Supervisors: Maria Teresa Dinis and Luís Conceição, Pedro Pousão-Ferreira (IPIMAR).
20. Santos, Rui. 2008. Optimization of methods of determination of isotopic ratios by icp-ms with
quadrupole and application to geological and environmental studies. PhD thesis in Chemistry,
Faculty of Sciences, University of Porto. Supervisor: Maria Teresa SD Vasconcelos, Co-supervisor:
Colombo Tassinari.
21. Serrano, Rui Manuel. 2008. Pheromones in the reproduction of Salaria pavo and S. fluviatilis
(Pisces: Blenniidae): a comparative study. PhD thesis, University of Évora. Supervisors: Eduardo
N. Barata, Adelino VM Canário and Peter C Hubbard.
22. Silva, Carina. 2008. Reproductive Ecology of the mildly sex-role reversed pipefish, Syngnathus
abaster (Pisces; Syngnathidae). PhD thesis in Biology, Faculty of Sciences, University of Porto.
Supervisors: Maria Natividade Vieira, Vitor Almada (ISPA).
23. Silva, Manuela. 2008. Metals and butyltins in sediments of Ria Formosa – The role of Spartina
maritima and Sarcocornia fruticosa. PhD thesis in Environmental Sciences and Technology,
Faculty of Sciences, University of Porto. Supervisor: Maria Teresa SD Vasconcelos, Co-supervisor:
M Caetano.
24. Sousa, Ronaldo. 2008. Factors contributing to the invasive success of Corbicula fluminea (Müller,
1774). PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Lúcia Guilhermino,
Co-supervisor: Carlos Antunes.
25. Stronowski, Zuzana. 2008. A high-resolution climate study on the Norwegian & Iberian shelves
during the Late Holocene: a diatom perspective. PhD thesis, University of Plymouth. Supervisor:
Fátima Abrantes.
26. Tiago, Daniel Martins. 2008. 2008. Role of insulin and insulin like peptides in bone formation:
identification of bone specific target genes and regulatory mechanisms, and characterization of
the insulin mimetic effect of vanadium. PhD thesis, University of Algarve. Co-supervisors: Maria
Leonor Cancela, Vicent Laizé and Manuel Aureliano.
MASTER THESES
1.
Afonso, Ricardo. 2008. Estudo sobre aproveitamento biotecnológico de organismos marinhos.
Master thesis, University of Algarve. Co-supervisors: Maria Leonor Cancela and Ricardo Leite.
2.
Aguiar Carvalho, Célia Cristina. 2008. Physiological performance of the Peppery furrow shell
Scrobicularia plana (da Costa 1778) along the European coast. Master in collaboration with the
Royal Netherlands Institute for Sea Research (NIOZ, Netherland), University of Tras-os-Montes
and Alto Douro (UTAD). Supervisor: Henk Van der Veer, Co-supervisor: Paulo Rema.
3.
Almeida, C. 2008. Distribution and shell pattern of hermit crabs in Algarve Coast. Master thesis,
Faculty of Marine Sciences and Environment, University of Algarve.
4.
Alves, Ricardo. 2008. Identificação de indicadores moleculares de bem-estar durante o cultivo de
Marine Biology, University of Algarve. Supervisors: Luís Conceição and Pedro Rodrigues.
5.
Andrade, Mariana. 2008. Avaliação dos efeitos morfológicos e ultraestruturais de microcistinas
em células Vero. Master thesis in Hydrobiology, Faculty of Sciences, University of Porto.
Supervisor: Vítor Vasconcelos, Co-supervisor: Paulo Pereira (INSA-Lisbon).
6.
Barros, Filipe. 2008. Utilização de assinaturas químicas elementares e isotópicas no estudo do
ciclo de vida do congro Europeu, Conger conger. Master thesis in Applied Ecology, Faculty of
Sciences, University of Porto. Supervisor: Alberto T Correira.
7.
Batalha, R. 2008. Coexistência e comportamento das lagostas de Cabo Verde. Master thesis in
Marine Biology, Faculty of Marine Sciences and Environment, University of the Algarve.
8.
Batista, Sara. 2008. The effect of the replacement of fishmeal to algae flour on growth and
9.
Bicho, Rita. 2008. Efeito do cloro no desenvolvimento e no eixo tiroide da Tilapia Moçambicana
(Oreochromis mossambicus). Master thesis in Biotechnology, University of Algarve.
10. Borges, Cristina. 2008. Estudos de adaptação ao alimento artificial em larvas de peixes ciclídeos
ornamentais: escalar (Pterophyllum scalare) e kribensis (Pelvicachromis pulcher). Master thesis in
Biology and Management of Marine Resources - Fisheries and Aquaculture, Faculty of Sciences,
University of Lisbon. Supervisor: AP Carvalho.
11. Carvalho, Edison. 2008. The influence of disruption of the thyroid hormone axis on skin: a
morpho-functional study. Master thesis in Marine Biology, University of Algarve.
12. Carvalho, Pedro. 2008. Research on ex-situ remediation by Halimione portulacoides of
contaminated estuarine sediments with butyltin compounds. Master thesis in Chemistry, Faculty
of Sciences, University of Porto. Supervisor: Maria Clara Basto.
13. Cimas, Cortes. 2008. Assessment of the Non-Indigenous Species in Ria Formosa. Erasmus
Mundus European Joint Master thesis in Water and Coastal Management, University of Algarve.
Supervisors: Aschwin Engelen, A Newton.
14. Coelho, Eucilene. 2008. Avaliação da qualidade ecológica do rio Leça. Master in thesis
Hydrobiology, Faculty of Sciences, University of Porto. Supervisor: Maria Natividade Vieira.
15. Costa, Ana. 2008. Variation of hemipelagic sedimentation in the submarine Cascais Canyon and
adjacent slopes for the last 150 Years. Master thesis, University of Lisbon.
16. Coxey, MS. 2008. Biological diversity and community structure in vessel reefs in the coast of
Recife (PE) – Brazil. Master thesis, Faculty of Marine Sciences and Environment, University of
Algarve.
17. Dias, Ana Cláudia. 2008. Influência de diferentes poluentes orgânicos na acumulação de cobre
por uma planta de sapal. Master thesis in Chemistry, Faculty of Sciences, University of Porto.
18. Dionísio, Gisela. 2008. Comportamento alimentar e resistência à inanação de larvas de camarões
ornamentais do Género Lysmata (Decapoda: Hippolytidae). Master thesis in Marine Biology,
University of Algarve. Supervisors: Maria Teresa Dinis and Ricardo Calado.
19. Duarte, Daniela. 2008. Parasitismo em Talorchestia brito (Stebbing, 1891) na Região Norte e
Centro da Costa Portuguesa. Master thesis, Faculty of Sciences, University of Porto. Supervisor:
Maria João Santos.
20. Fernandes, Sandra. 2008. Estudo dos mecanismos de destoxificação de microcistinas em
bivalves. Master thesis in Applied Ecology, Faculty of Sciences, University of Porto. Supervisor:
Vítor Vasconcelos.
21. Fernandez-Carvalho, J. 2008. Application of underwater video techniques for the characterization
of underwater benthic communities. Master thesis, Faculty of Marine Sciences and Environment,
University of the Algarve.
22. Ferraz, Marco. 2008. Caracterização das dunas e campos dunares da Parte Norte da Península de
Tróia. Master thesis, University of Lisbon.
23. Ferreira, A. 2008. Lipofuscin concentration versus length as indicators of age in decapods. Using
the Norway lobster (Nephrops norvegicus) as a model. Master thesis in Marine Biology, Faculty of
Marine Sciences and Environment, University of the Algarve.
24. Ferreira, Clara. 2008. Molecular and sub-cellular approaches in soil ecotoxicology. Master thesis
in Molecular and Cell Biology, University of Aveiro. Supervisor: Amadeu MVM Soares (CESAM,
University of Aveiro); Co-supervisors: Carlos Gravato, Mónica Amorim (CESAM, University of
Aveiro).
25. Fragoso, Bruno. 2008. Assessment of biofouling in PVC recruitment surfaces and efficiency of non
toxic materials in the construction of nets in an oyster aquaculture farm in Sagres. Master thesis
in Marine Biology, University of Algarve. Supervisors: Ester Serrão, John Icely.
26. Frazão, Bárbara. 2008. Ecotoxicologia de cianobactérias marinhas da região centro de Portugal.
Master thesis in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor: Vítor
Vasconcelos.
27. Garcia, Eufémia. 2008. Gestão de solventes na indústria de fabrico e impressão de embalagens
metálicas. Master thesis in Environmental Sciences and Technology, Faculty of Sciences,
University of Porto. Supervisor: Maria Teresa SD Vasconcelos.
28. Gonçalves, Odete. 2008. Gastric proton pump expression in teleost fishes: nongastric sites of
Douro (UTAD). Supervisor: Jonathan Wilson, Co-supervisor: António Fontaínhas Fernandes
(UTAD).
29. Gregoria, Silvia. 2008. Molecular and cellular analysis of the regenerative response of skin in a
fish model. Master thesis in Biological Engineering, University of Algarve.
30. Guerreiro, Eduarda. 2008. Thyroid Axis disruption by goitrogens: a molecular and functional
approach. Master thesis in Biological Engineering, University of Algarve.
31. Guimarães, ALG. 2008. Elaboração de uma ferramenta de ajuda à tomada de decisão no âmbito
do Regulamento de Sistemas Energéticos de Climatização de Edifícios no que concerne à
apreciação das metodologias de avaliação da Qualidade do Ar Interior. Master thesis in
Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor:
Maria Teresa SD Vasconcelos.
32. Hamidov, Amhad. 2008. Salt removing species: Fitoremediation technique for Uzbekistan.
Erasmus Mundus European Joint Master thesis, University of Algarve. Supervisor: Maria Clara
Costa.
33. Jordão, Vera. 2008. Tolerância à amónia e grau de ureoltelia no xarroco, Halobatrachus
dudactylus (Bloch & Schneider, 1801). Master thesis in Marine Biology, University of Algarve.
34. Leitão, Tânia Angelina. 2008. Efeito do caudal na estrutura das comunidades de macrocrustaceos
no estuário do Guadiana. Master thesis, University of Algarve. Supervisors: Maria Alexandra
Chicharo and Luís Chicharo.
35. Madeira, Celine. 2008. Contribuição parental do linguado do senegal, Solea senegalensis (KAUP
1858), em aquacultura. Master thesis, University of Algarve. Co-supervisor: Maria Leonor Cancela
and S Mira.
36. Marin, Patricia. 2008. Defining seagrass ecological status in Ria Formosa under the European
Water Framework Directive. Erasmus Mundus European Joint Master thesis in Water and Coastal
Management, University of Algarve. Supervisors: Susana Cabaço, A Newton.
37. Martins, Ana Sofia. 2008. Os macroinvertebrados bentónicos como indicadores da qualidade da
água de duas ribeiras (Ribeira da Madalena e Ribeira de Canelas). Master thesis in Hydrobiology,
Faculty of Sciences, University of Porto. Supervisor: Maria Natividade Vieira.
38. Martins, Joana. 2008. Estudo das comunidades de cianobactérias de uma ETAR (Águas de Gaia).
Quality Control, Faculty of Pharmacy, University of Porto. Supervisor: Vítor Vasconcelos, Cosupervisor: Luísa Peixe (Faculty of Pharmacy, University of Porto).
39. Martins, MAS. 2008. Effects of pollutants and natural stressors on populations of planktonic
organisms. Master thesis in Biology, University of Aveiro. Supervisor: Lúcia Guilhermino, Cosupervisor: Amadeu MVM Soares (University of Aveiro, CESAM).
40. Martins, Susana. 2008. Characterization of tobacco smoke produced/commercialized in Portugal.
Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor: Maria Teresa SD
Vasconcelos.
41. Mendes, B. 2008. Can reef conservation programs help reduce SCUBA diving damage to coral
reefs? A case study in Thailand. Master thesis in Marine Biology, Faculty of Marine Sciences and
Environment, University of Algarve.
42. Mota, Emilio António. 2008. Evaluation of enzymatic and metabolic parameters of quality in sole
eggs (Solea senegalensis, Kaup, 1858). Master thesis in Marine Biology, University of Algarve.
Supervisors: Maria Teresa Dinis, F Soares and Elsa Cabrita.
43. Nunes, Ana Isabel. 2008. Qualidade da água residual - monitorização através de respostas
ecológicas de protozoários e remoção de amónia e azoto total utilizando Chlorella vulgaris.
Master thesis, Faculty of Sciences, University of Porto.
44. Nunes, Cristovão José. 2008. Optimização do cultivo momoclonal da anemona vidro Aiptasia
pallida (Agassiz in Veriil, 1864), com ênfase para a importância dos ácidos gordos. Master thesis
in Marine Biology, University of Algarve. Supervisors: Maria Teresa Dinis and Ricardo Calado.
45. Peixoto, Marta Juliana. 2008. Avaliação da qualidade biológica da água do rio Cávado. Master
thesis in Hydrobiology, Faculty of Sciences, University of Porto. Supervisor: Maria Natividade
Vieira.
46. Pozdniakova, Tatiana. 2008. Ecoeficiência empresarial e desenvolvimento de ferramentas de
análise. Master thesis in Environmental Sciences and Technology, Faculty of Sciences, University
of Porto. Supervisor: Maria Teresa SD Vasconcelos.
47. Rainha, Rita. 2008. Estudo da Variabilidade genética de marcadores moleculares ligados ao gene
da MGP e sua aplicação para paternidades em Sparus aurata. Master thesis in Marine Biology,
University of Algarve. Co-supervisors: Maria Leonor Cancela and S Mira.
48. Rangel, Luis Filipe. 2008. Parasitas metazoários de poliquetas (Nereis diversicolor e Diopatra
neapolitana) da Ria de Aveiro. Master thesis in Applied Ecology, Faculty of Sciences, University of
Porto. Supervisor: Maria João. Santos.
49. Ribeiro, I. 2008. Amálise da distribuição do esforço de pesca e das capturas de palmeta
(Reinhardtius hippoglossoides, Walbaum 1792) capturada pela frota commercial Portuguesa na
área de regulamentação NAFO. Master thesis in Aquaculture and Fisheries, Faculty of Marine
Sciences and Environment, University of Algarve.
50. Rodrigues, Joana. 2008. A avifauna do estuário do Rio Minho (aves aquáticas). Caracterização,
importância e perspectivas de uso turístico. Master thesis in Sciences of the Sea – Sea Resources,
ICBAS, University of Porto. Supervisor: António Manuel da Silva Luís (University of Aveiro), CoSupervisor: Ana Bio.
51. Rodrigues, Pedro Nuno. 2008. Quantificação de poluentes orgânicos persistentes (POPs)
organoclorados em sedimentos e investigação ex-situ do papel da planta Halimione portulacoides
na respectiva bioremediação. Master thesis in Chemistry, Faculty of Sciences, University of Porto.
52. Rodrigues, Vera Lúcia. 2008. Nutritional modulation of innate immune parameters in the
epidermal mucus of Senegalese sole (Solea senegalensis). Master thesis in Marine Biology,
University of Algarve. Supervisors: Maria Teresa Dinis, Jorge Dias and L Ribeiro.
53. Rosa, Inês. 2008. Influence of high stocking density, fasting and refeeding on intermediate
Algarve.
54. Severino, Ricardo 2008. Contributo para o conhecimento do ciclo de vida de Amyloodinium
ocellatum e seu controlo em piscicultura. Master thesis, Faculty of Sciences, University of Porto.
Supervisor: Maria João Santos.
55. Silva, Patrícia. 2008. Efeitos de cilindrospermopsina na germinação e crescimento de sementes
de alface. Master thesis in Environmental Sciences and Technology, Faculty of Sciences,
University of Porto, Supervisor: Vítor Vasconcelos.
56. Silva, Polyana. 2008. Gnotobiotic growth system model for Dicentrarchus labrax larvae. Master in
Marine Biology, University of Algarve. Supervisors: Maria Teresa Dinis and P Bossier.
57. Timóteo, Viriato. 2008. Reprodução do pargo (Pagrus pagrus Linnaeus, 1758) em cativeiroavaliação da qualidade das posturas. Master thesis in Sciences of the Sea - Sea Resources, ICBAS,
University of Porto. Supervisors: Maria Teresa Dinis and C Andrade.
C.
COMMUNICATIONS
COMMUNICATIONS IN INTERNATIONAL SCIENTIFIC MEETINGS
1.
Abrantes, F, Montanari, B, Rodrigues, T, Santos, C, Voelker, A. 2008. Primary productivity
evolution during the Latest Holocene (2,000 yr) off Oporto (Portuguese Margin). European
Geosciences Union General Assembly, 13-18 April, Vienna, Austria. Geophysical Research
Abstracts, vol. 10, EGU2008-A-04305.
2.
Abrantes, F, Lopes, C, Amigoni, E. 2008. The last deglaciation paleoproductivity and paleoclimate
off southern Chile: a diatom (accumulation rates, assemblages and transfer functions) view.
International Symposium on Eastern Boundary Upwelling Ecosystems, 2-6 June, Gran Canaria,
Spain. Abstract book, pp. 79-80.
3.
Abreu, MH, Pereira, R, Sousa-Pinto, I, Yarish, C. 2008. Life History studies and N uptake
performances of Gracilaria vermycolopjylla (Ohmi) Papenfuss: preliminary assessment. 11th
International Conference on Applied Phycology, 22-27 June, Galway, Ireland.
4.
Afonso, A. 2008. Review of aquaculture development in Portugal and Europe. Proceedings of the
International Workshop: Developing a Sustainable Aquaculture Industry in the Azores, 2-5 June,
Horta, Azores, Portugal. (Oral communication)
5.
Almeida, AP, Frattani, FS, Zingali, RB, Chaves, DSA, Costa, SS, Pinto, M, Naengchomnong, W,
Kijjoa, A. 2008. The anticoagulant and antithrombotic profile of a root methanolic extract from
Strophioblachia fimbricalyx Boerl (Euphorbiaceae). 7th Joint Meeting of AFERP, ASP, GA, PSE &
SIF - Natural Products with Pharmaceutical, Nutraceutical, Cosmetic and Agrochemical Interest,
3-8 August, Athens, Greece.
6.
Almeida, CMR, Dias, AC, Mucha, AP, Bordalo, A, Vasconcelos, MTSD. 2008. Influence of organic
pollutants on the phytoextraction potential of Halimione portulacoides. 3rd International
Meeting on Environmental Biotechnology and Engineering (3IMEBE), 21-25 September, Palma de
Mallorca, Spain. (Oral communication)
7.
Almeida, J, Gravato, C, Guilhermino, L. 2008. Effects of benzo-a-pyrene in seabass (Dicentrarchus
labrax): accumulation, biomarkers and oxidative damage in muscle and brain. 29th Annual
Meeting of the Society of Environmental Toxicology and Chemistry - SETAC North America, 16-20
November, Tampa, Florida, USA. Abstract book, p. 315.
8.
Almeida, R, Kaczor, A, Gomez-Zavaglia, A, Cristiano, MLS, Fausto, R. 2008. Chapman-Type
Rearrangement in 3-(Methoxy)-1,2-Benzisothiazole 1,1-Dioxide through an Intramolecular
Mechanism in Condensed Phases. 19th IUPAC Conference on Physical Organic Chemistry, 13-17
July, Santiago de Compostela, Spain.
9.
Amorim, M, Scott-Fordsmand, J, Gomes, S, Novais, S, Soares, A, Gravato, C, Guilhermino, L. 2008.
Effects of copper nanoparticles on oxidative stress biomarkers in Enchytraeus albidus. 5th World
Congress of the Society of Environmental Toxicology and Chemistry, 3-7 August, Sydney,
Australia.
10. Anjos, L, Canário, AVM, Power, DM. 2008. Cloning and production of puffer fish (Tetraodon
negroviridis and Fugu rubripes) recombinant Parathyroid Hormone Proteins (PTHs). 6th
International Symposium on Fish Endocrinology, 22-27 June, Calgary, Canada.
11. Antunes, P, Amado, J, Pousão-Ferreira, P, Reis-Henriques, MA. 2008. Níveis de compostos
organoclorados numa nova espécie de aquacultura sargo legítimo (Diplodus sargus). CICTA 2008
- 7th Iberian and 4th Iberoamerican Congress on Environmental Contamination and Toxicology,
10-12 March, Lisbon, Portugal.
12. Antunes, P, Gil, O, Hendriks, AJ, Huijbregts, MAJ, Reis-Henriques, MA. 2008. Comparing
elimination pattern of PCBs in juvenile and adult seabass (Dicentrarchus labrax). DIOXIN 2008 28th International Symposium on Halogenated Environmental Organic Pollutants (POPs), 17-22
August, Birmingham, UK. Organohalogen Compounds 70: 1989-1992.
13. Antunes, P, Gil, O, Reis-Henriques, MA. 2008. Accumulation of PCB metabolites in plasma of
seabass (Dicentrarchus labrax). DIOXIN 2008 - 28th International Symposium on Halogenated
Environmental Organic Pollutants (POPs), 17-22 August, Birmingham, UK. Organohalogen
Compounds 70: 1985-1988.
14. Araújo, N, Cristiano, ML, O’Neill, PM. 2008. Plasmodium DNA-Directed Endoperoxides. 22th
International Symposium on Medicinal Chemistry, 31 August-5 September, Vienna, Austria.
15. Baptista, MS, Stoichev, T, Basto, MCP, Vasconcelos, MTSD. 2008. In vitro octylphenol interaction
with cyanobacterium Microcystis aeruginosa. 3rd International Symposium on Environment, 2225 May, Athens, Greece. (Oral communication)
16. Barreiro, J, Madureira, T, Lourenço, K, Rocha, MJ, Tiritan, ME, Cass, Q. 2008. Avaliação do uso de
colunas do tipo “Restricted-Access Medium” (RAM) na exclusão de macromoléculas em amostras
de águas de estuário e esgoto doméstico. COLACRO XII - Congresso Latino-Americano de
Cromatografia e Técnicas Relacionadas, 28-30 October, Florianópolis, Brazil.
17. Barrento, S, Teixeira, B, Marques, A, Vaz-Pires, P, Nunes, ML. 2008. Simulated live shipment of
edible crab Cancer pagurus: influence of temperature, air exposure and anesthetic on
physiological stress. 38th WEFTA Annual Meeting, WEFTA2008, 17-19 September, Florence, Italy.
(Oral communication)
18. Barrento, S, Teixeira, B, Marques, A, Carvalho, ML, Vaz-Pires, P, Nunes, ML. 2008. Contaminants
in two populations of edible crab C. pagurus: environmental and human health implications. 38th
WEFTA Annual Meeting, WEFTA2008, 17-19 September, Florence, Italy. (Oral communication)
19. Barrento, S, Teixeira, B, Marques, A, Vaz-Pires, P, Nunes, ML. 2008. Chemical composition of
Cancer pagurus from two locations: Scottish coast and English Channel. First International
Conference of Seafood Technology, 18-21 May, Cesme, Izmir, Turkey. (Oral communication)
20. Bastos, L, Bos, MS, Fernandes, RMS. 2008. Investigating the seasonal signal within GPS timeseries from Iberia stations. 14th General Assembly of WEGENER - WEGENER 2008, 15-18
September, Darmstadt, Germany. (Oral communication)
21. Bastos, L, Silva, J. 2008. Use of GPS based drifters in the study of coastal currents. 13th FIG
Symposium on Deformation Measurements and Analysis and 4th IAG Symposium on Geodesy for
Geotechnical and Structural Engineering, 12-15 May, Lisbon, Portugal.
22. Ben-Hamadou, R, Chicharo, A, Madeira, C, Morais, P, Cruz, J, Chicharo, L. 2008. Zooplankton
distribution in Algarve coastal zone (North East Atlantic Ocean). Joint WGZE/CIESM Workshop to
Compare Zooplankton Ecology and Methodologies between the Mediterraean and the North
Atlantic (WKZEM), 27-30 October, Heraklion, Greece.
phytogeography of the Portuguese continental coast. 43rd Estuarine & Coastal Sciences
Association International Symposium, 7-9 February, Lisbon, Portugal.
phytogeography of the Portuguese continental coast. ASLO Summer Meeting, 8-13 June, St
John’s, Newfoundland & Labrador, Canada.
25. Billard, E, Serrao, ETA, Pearson, GA, Destombe, C, Roze, D, Daguin, C, Neiva, J, Engel, C, Valero,
M. 2008. Evolution of mating systems and their implications in the processes of speciation and
hybridization in brown algae of the genus Fucus. Marine Genomics Europe Final Assembly, 13-16
May, Faro, Portugal.
26. Boehm, J, Bos, M, King, M, Lidberg, M, Makinen, J, Mendes Cerveira, PJ, Penna, N, Schuh, H,
Steigenberger, P, Vittuari, L, Willis, P. 2008. Geodetic observation level modelling for the
measurement of GIA. American Geophysical Union Fall Meeting 2008, 14-18 December, San
Francisco, USA. Abstract ref. #G31A-0647.
27. Borges, R, Arnaud, S, Gonçalves, EJ, Serrão, ME. 2008. Testing the suitability of a new fixative
both for morphological identification and DNA extraction of fish larvae. 32nd Larval Fish
Conference, 4-7 August, Kiel, Germany.
28. Bos, MS, Deurloo, RA, Bastos, L. 2008. Project AGMASCO: Noise investigation of airborne
gravimetry observations. Luso-Spanish Assembly of Geodesy and Geophysics, 11-14 February,
Tomar, Portugal.
29. Bos, MS, Penna, NT, Baker, TF, Scherneck, H-G, Bastos, L. 2008. Current state of ocean tide
loading displacement modeling. European Geosciences Union General Assembly, 13-18 April,
Vienna, Austria. Geophysical Research Abstracts, vol. 10, EGU2008-A-05142. (Oral
communication)
30. Bos, MS, Penna, NT, Baker, TF, Scherneck, H-G. 2008. Assessing the accuracy of predicted ocean
tide loading displacement values. Earth Tide Symposium, 1-5 September, Jena, Germany. (Oral
communication)
31. Brito, AB, Dionísio, G, Gavaia, PJ, Cancela, ML. 2008. Expression of bone-related genes during
regeneration of zebrafish (Danio rerio) fins. 2nd Meeting of the European Society for
Evolutionary Development Biology, 29 July-1 August, Ghent, Belgium. (Oral communication)
32. Brito, AB, Leite, RB, Cancela, ML. 2008. Hypoxia-Inducible Factor 1α expression profile in gilthead
seabream (Sparus aurata). Marine Genomics Europe Final Assembly, 13-16 May, Faro, Portugal.
33. Brito, P, Duarte, H, Terrinha, P, Rebêlo, L, Ferraz, M. 2008. Late quaternary coastline variations
between Tróia and Cape Espichel from new geological and geophysical data. PoCoast Seminar on
Coastal Research, 26-28 May, Porto, Portugal.
34. Cabrita, E, Soares, F, Beirão, J, Aragão, C, Diogo, P, Madeira, C, Mira, S, Dinis, MT. 2008. Male
reproductive performance in Solea senegalensis. IV Workshop on the Cultivation of Soles, 11-13
November, Faro, Portugal. (Oral communication)
35. Caldeira, RMA, Luis, E, Santos, A, Videman, J. 2008. Madeira Island wakes. ROMS/TOMS
European Workshop, 6-8 October, Grenoble, France. (Oral communication)
36. Caldeira, RMA, Riha, S, Santos, A. 2008. Madeira Island wakes: barotropic instability study.
International Conference on Mathematics and Continuum Mechanics, 19-22 February, Porto,
Portugal. (Oral communication)
37. Caldeira, RMA, Luis, E, Boutov, D, Santos, A, Videman, J, Chelius, A, Sangrá, P. 2008. Island wakes:
Madeira Archipelago case study. Navy Research Laboratory: Code 7320, Ocean Dynamics and
Prediction Branch, October, Mississippi, USA. (Oral communication)
38. Canário, AVM, Pinto, PIS. 2008. Omics approach in marine biology. 24th Conference of European
Comparative Endocrinologists, 2-6 September, Genoa, Italy. (Oral communication)
39. Cardeira da Silva, J, Gavaia, PJ, Brito, AB, Cancela, ML. 2008. The use of enhanced green
fluorescent protein to track matrix Gla protein expression during zebrafish (Danio rerio)
development. Marine Genomics Europe Final Assembly, 13-16 May, Faro, Portugal. (Oral
communication)
40. Cardoso, JCR, Vieira, F, Gomes, AS, Power, DM. 2008. A revised history of secretin family
evolution. Genomes to Systems 2008, 17-19 March, Manchester, UK.
41. Carrola, J, Fontaínhas-Fernandes, A, Teixeira, P, Rocha, E. 2008. Frequency of hepatocellular fibrillar
inclusions in the liver of flounders (Platychthis flesus) from the Douro estuary, Portugal. 18th Annual
Meeting of the Society of Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May,
Warsaw, Poland.
42. Carvalho, C, Alves, S, Gomes, C, Pacheco, S, Soares, C, Couto, C, Rocha, MJ. 2008. Monitorization
of N-cycle parameters in waters of Leça River Estuary (Portugal). 18th Annual Meeting of the
Society of Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw, Poland.
43. Carvalho, C, Conrado, AR, Neto, I, Rodrigues, S, Pinto, I, Rocha, MJ, Couto, C. 2008. Organic
pollution and heavy metal assessment in waters of Leça River Estuary (Portugal). 18th Annual
Warsaw, Poland.
44. Carvalho, P, Rodrigues, PNP, Basto, MCP, Vasconcelos, MTSD. 2008. Levels of butyltins in
sediments of different Portuguese coastal areas. SEASINK, 26-28 June, Porto, Portugal
45. Castro, LFC, Lima, D, Machado, A, Reis-Henriques, MA, Santos, MM. 2008. Molecular
characterization of 17-β hydroxysteroid dehydrogenase type 12 (HSD17B12) in the neogastropod
Nucella lapillus. 24th Conference of European Comparative Endocrinologist, 2-6 September,
Genoa, Italy. (Oral communication)
46. Coimbra, J, Moreira, SM. 2008. Ocean Policies for the 21st Century. SEASINK, 26-28 June, Porto,
47. Coimbra, J, Moreira, SM. 2008. CIMAR Associate Laboratory. General Assembly of Marine Board European Science Foundation, BioMarine 2008 - The Europe Maritime Iniciative in the World:
Dive into Change,20-21 October, Toulon, France. (Oral communication)
48. Conceição, L, Costas, B, Aragão, C, Dinis, MT. 2008. Stressful husbandry conditions and amino
acid requirements in Senegalese sole (Solea senegalensis). COST Action 867, Workshop on
Welfare of fish in European Aquaculture, 14-17 April, Krakow, Poland.
49. Conceição, LEC, Dias, J, Costas, B, Saavedra, M, Silva, JMG, Valente, LMP, Espe, M. 2008. A
framework to assess indispensable amino acids requirements in fish: integration of the ideal
protein concept and tracer studies. XIII Symposium on Fish Nutrition and Feeding, 1-5 June,
Florianópolis, Brazil. (Oral communication)
50. Conceição, N, Fazenda, C, Simões, B, Cancela L. (2008). Transcriptional regulation of the Xenopus
laevis matrix Gla protein gene expression. 35th European Symposium on Calcified Tissues, 24-28
May, Barcelona, Spain.
51. Conceição, N, Laizé, V, Cancela, ML. 2008. Regulation of matrix Gla protein gene expression in
teleost fish Sparus aurata. Marine Genomics Europe Final Assembly, 13-16 May, Faro, Portugal.
52. Conceição, N, Fazenda, C, Simões, B, Cancela, L. 2008. Transcriptional regulation of the Xenopus
laevis Matrix Gla protein gene. 35th European Symposium on Calcified Tissues, 24-28 May,
Barcelona, Spain. Calcified Tissue International 82: S139.
53. Cooper, C, Wilson, J, Wilson, R. 2008. Acid-base and osmoregulatory adaptations by the fish gill
and gut at hypersalinities: An immunohistochemical approach. Society for Experimental Biology
Annual Main Meeting, 6-10 July, Marseille, France. Comparative Biochemistry and Physiology
Part A 150(3), Supplement 1: S111.
54. Costa, A, Mil-Homens, M, Lebreiro, S, Trancoso, A, Mouro, F, Mateus, M, de Stigter, H, Boer, W,
Richter, T, van Weering, TCE, Abrantes, F. 2008. Pb Enrichments in short sediment cores collected
off the Portuguese Margin. XIV Seminário Ibérico de Química Marinha, 22-24 September, Cádiz,
Spain. (Oral communication)
55. Costa, J, Ferreira, M, Pousão, P, Reis-Henriques, MA. 2008. Biomarkers of exposure as a tool for
evaluating the quality of farmed and wild European seabass and white seabream. CICTA 2008 7th Iberian and 4th Iberoamerican Congress on Environmental Contamination and Toxicology,
56. Costas, B, Aragão, C, Dinis, MT, Afonso, A, Conceição, L. 2008. Stressful rearing conditions and
amino acid nutrition in Senegalese sole. IV Workshop on the Cultivation of Soles, 11-13
November, Faro, Portugal. (Oral communication)
57. Costas, B, Aragão, C, Míguez, JM, Soengas, JL, Conceição, LEC. 2008. Preliminary results on effects
of amino acids supplementation on stress response and serotonergic activity in Solea
senegalensis. COST Action 867, Workshop on Welfare of fish in European Aquaculture, 14-17
April, Krakow, Poland. (Oral communication)
58. Costas, B, Valente, L, Conceição, LEC. 2008. Indispensable amino acids absorption and retention
in Senegalese sole (Solea senegalensis, Kaup 1858) juveniles: In vivo tracer studies. XIII
Symposium on Fish Nutrition and Feeding, 1-5 June, Florianópolis, Brazil.
59. Couto, C, Carvalho, C, Alves, S, Gomes, C, Pacheco, S, Soares, C, Rocha, MJ. 2008. Wastewater
treatment plant impact on N-cycle parameters in river waters. AOAC Europe Section
International Workshop: Enforcement of European Legislation on Food and Water - Analytical
and Toxicological Aspects, 17-18 April, Lisbon, Portugal.
60. Couto, C, Pinto, I, Carvalho, C, Conrado, AR, Neto, I, Rodrigues, S, Rocha, MJ. 2008. Temporal and
spatial monitorization of organic and metal pollution and N-cycle parameters in waters of Leça
River Estuary (Portugal). AOAC Europe Section International Workshop: Enforcement of
European Legislation on Food and Water - Analytical and Toxicological Aspects, 17-18 April,
Lisbon, Portugal.
61. Couto, MN, Vasconcelos, MTSD. 2008. Case study: remediation of hydrocarbons from a refinery’s
soil - a biological approach. ConSoil Conference, 3-6- June, Milan, Italy.
62. Cristiano, MLS. 2008. Studies on the photochemistry of tetrazoles in criogenic inert matrices and
in solution. 19th IUPAC Conference on Physical Organic Chemistry, 13-17 July, Santiago de
Compostela, Spain. (Oral communication)
63. Cristiano, MLS. 2008. Synthesis and applications of novel saccharyl-tetrazoles as multidentate
ligands. Symposium in Honour of Eric Derouane, 25-26 September, Lisbon, Portugal. (Oral
communication)
64. Cruz, J, dos Santos, A, Pastor, J, Santos, AM. 2008. Zooplankton seasonal variation at an
upwelling shadow coastal station off Western Iberia: relation to environmental conditions.
Eastern Boundary Upwelling Ecosystems Symposium, 2-6 June, Las Palmas, Gran Canaria, Spain.
65. Cruzeiro, L. 2008. Exploring protein's multifunnel energy landscape, 1st Portuguese-SpanishBritish Joint Biophysics Congress, 10-13 July, Lisbon, Portugal. Abstract book, p. 151.
66. Da Silva, AJ, Martins, I, Bastos, L. 2008. Northwest Iberian Coastal Current: an extreme event
feature. ECSA 43rd Local Meeting Symposium on Climate Change Impacts on South-European
Coastal Ecosystems, 7-9 February, Lisbon, Portugal. (Oral communication)
67. Da Silva, AJ, Martins, I, Oliveira, A, Bastos, L. 2008. An intermittent Northwest Iberian Coastal
Current. 5th EuroGOOS Conference on Coastal to Global Operational Oceanography:
Achievements and Challenges, 20-22 May, Exeter, UK. (Oral communication)
68. Da Silva, AJ, Martins, I, Santos, A, Bastos, L. 2008. NW Iberian Coastal Current: a feature of
extreme freshwater and wind conditions. International Symposium on the Effects of Climate
Change on the World's Oceans, 19-23 May, Gijón, Spain. (Oral communication)
69. Dias, AC, Almeida, CMR, Mucha, AP, Bordalo, A, Vasconcelos, MTSD. 2008. Influence of organic
pollutants on the accumulation of copper by a salt marsh plant. SEASINK, 26-28 June, Porto,
Portugal.
70. Dias, E, Amorim, A, Carvalho, F, Santos, MJ, Antunes, JC. 2008. Ectoparasites survey in young
European flounder (Platichthys flesus) on Minho estuary. 7th International Flatfish Symposium,
Sesimbra, Portugal.
71. Dias, J, Aires, T, Borges, P, Conceição, L, Costas, B, Cunha, M, Engrola, S, Espe, M, Guerreiro, I,
Silva, J, Valente, L, Yúfera, M, Dinis, MT. 2008. Formulation of practical diets for Senegalese sole.
IV Workshop on the Cultivation of Soles, 11-13 November, Faro, Portugal. (Oral communication)
72. Dias, SC, Sousa, R, Freitas, V, Vale, M, Vasconcelos, V, Antunes, C. 2008. Aestival characterization
of the River Minho estuary aquatic communities. IV Iberian Symposium of the River Minho
Hydrological Basin, 14-15 November, Vila Nova de Cerveira, Portugal. (Oral communication)
73. Dionísio, G, Brito, AB, Gavaia, PJ, Cancela, ML. 2008. Diets affect skeletal development in
zebrafish (Danio rerio). Marine Genomics Europe Final Assembly, 13-16 May, Faro, Portugal.
74. Diekmann, OE, Alberto, F, Gouveia, L, Duarte, C, Serrão, EA. 2008. Genetic diversity and
differentiation in the seagrass species Zostera noltii and Cymodocea nodosa across the AtlanticMediterranean divide. ISBW8, 1-5 September, BMSC Bamfield, Vancouver Island, Canada.
75. Diekmann, OE, Alberto, F, Gouveia, L, Serrão, EA. 2008. Genetic diversity of Zostera noltii
populations under various levels of disturbance. ISBW8, 1-5 September, BMSC Bamfield,
Vancouver Island, Canada.
76. Ellegaard, M, Lundholm, N, Ribeiro, S, Ekelund, F, Thorbjørn, J. 2008. Long-term survival of
dinoflagellate cysts in anoxic marine sediments. 8th International Conference on Modern and
Fossil Dinoflagellates - Dino8, 4-10 May, Montreal, Canada.
77. Fernandes, MJ, Nunes, AL, Lázaro, C, Pires, N, Bastos, L, Mendes, VB. 2008. Wet tropospheric
correction for coastal altimetry based on GNSS path delay measurements. 2nd Workshop on
Coastal Altimetry, 6-7 November, Pisa, Italy. (Oral communication)
78. Fernandes, MJ, Nunes, AL, Lázaro, C, Pires, N, Bastos, L, Mendes, VB. 2008. The wet tropospheric
correction for coastal altimetry based on GNSS path delay measurements. Observing and
Forecasting the Ocean - OSTST Annual Meeting, 10-15 November, Nice, France.
79. Fernandes, RMS, Miranda, JM, Bastos, L, Catalão, J, Bos, MS. 2008. Evaluating the present-day
kinematics of Azores Triple Junction by combining campaign and permanent GNSS data. 2008
UNAVCO Science Workshop, 11-13 March, Colorado, USA. (Oral communication)
80. Ferreira, M, Costa, J, Caetano, M, Pousão, P, Vale, C, Reis-Henriques, MA. 2008. Oxidative Stress
biomarkers in cultured and wild white sea bream (Diplodus sargus). Can they be useful to
evaluate cultured fish quality?. CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress on
Environmental Contamination and Toxicology, 10-12 March, Lisbon, Portugal.
81. Fidalgo, ML, Valente, A, Vieira, N. 2008. Estado ecológico das pequenas ribeiras costeiras entre o
rio Minho e a barrinha de Esmoriz. 1st PoCoast Seminar on Coastal Research, 26-28 May, Porto,
82. Francisco, CJ, Almeida, AS, Castro, AM, Santos, MJ. 2008. Desenvolvimento de marcador
molecular, RFLP, para distinção genética de cercárias de Prosorynchus crucibulum e P. aculeatus
(Trematoda: Digenea). X ENBRAPOA, 17-20 November, Búzios, Brazil.
83. Frasco, MF, Erzen, I, Stojan, J, Guilhermino, L. 2008. Localization and properties of
cholinesterases in the common prawn (Palaemon serratus): a kinetic-histochemical study. CICTA
2008 - 7th Iberian and 4th Iberoamerican Congress on Environmental Contamination and
Toxicology, 10-12 March, Lisbon, Portugal.
84. Freitas, P, Clarke, LJ, Kennedy, HA, Richardson, CA. 2008. Experimental calibration of the oxygenisotope paleotemperature proxy in the shells of the bivalves Mytilus edulis and Pecten maximus.
ESF-MEDCLIVAR Oxygen isotopes as tracers of Mediterranean climate variability, 11-13 July, Pisa,
Italy.
85. Frija, LMT, Loureiro, RMS, Fausto, R, Cristiano, MLS. 2008. Recent advances in the synthesis and
applications of new saccharinate-tetrazoles as multidentate ligands. 19th IUPAC Conference on
Physical Organic Chemistry, 13-17 July, Santiago de Compostela, Spain.
86. Gadelha, JR, Vieira, L, Humanes, M, Guilhermino, L, Soares, AMVM, Morgado, F. 2008. Is
Anemonia sulcata (Forskål, 1775) a suitable sentinel species?. CICTA 2008 - 7th Iberian and 4th
Iberoamerican Congress on Environmental Contamination and Toxicology, 10-12 March, Lisbon,
Portugal.
87. Gadelha, JR, Vieira, L, Humanes, M, Guilhermino, L, Soares, AMVM, Morgado, F. 2008. The
activity of cholinesterases and glutathione S-transferases of Anemonia sulcata (Forskål, 1775) as
environmental biomarkers in the Portuguese coast. 18th Annual Meeting of the Society of
Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw, Poland. Abstract
book, p. 122.
88. Garcia, P, Casal, G, Martins, ML, Matos, E, Azevedo, C. 2008. Ultraestrutura do esporo de
Chloromyxum sp. (Myxozoa: Myxosporea) encontrado na vesícula biliar da raia-santa Rioraja
agassizi (Elasmobranchii: Rajidae). X ENBRAPOA, 17-20 November, Búzios, Brazil.
89. Garcia-Santos, S, Fontainhas-Fernandes, A, Mancera, J, Wilson, JM. 2008. Metabolic and
osmoregulatory alterations and cell proliferation in gilthead sea bream (Sparus aurata) exposed
to cadmium. Society Society for Experimental Biology Annual Main Meeting, 6-10 July, Marseille,
France. Comparative Biochemistry and Physiology Part A 150(3), Supplement 1: S105.
90. Garrido, S, Rosa, R, Bem-Hamadou, R, Cunha, E, Chícharo, A, van der Lingen, C. 2008. Spatiotemporal variability in fatty acid trophic biomarkers in stomach contents and muscle of Iberian
sardine (Sardina pilchardus) and its relationship with upwelling and spawning. Symposium on
Eastern Boundary Upwelling Ecosystems, 2-6 June, Las Palmas, Spain. Abstract book, p. 197
91. Gavaia, PJ. 2008. Avaliação esquelética de tratamentos nutricionais em Linguado. Jornada de
divulgação e transferência de resultados de cooperação transfronteriça Algarve-Andaluzia para a
investigação e promoção de novos produtos aquicolas. IFAPA Centro Agua del Pino, 29 April,
Cartaya, Spain. (Oral communication)
92. Gavaia, PJ. 2008. Fish as a model organism to study skeletal development and associated
pathologies. XXXVII Annual Meeting of the Brazilian Society for Biochemistry and Molecular
Biology (SBBq) - XI Congress of the Panamerican Association for Biochemistry and Molecular
Biology (PABMB), 17-20 May, Águas de Lindóia, São Paulo, Brazil. (Oral communication)
93. Gavaia, PJ. 2008. Skeletal evaluation in early stages of Solea senegalensis. IV Workshop on the
Cultivation of Soles, 11-13 November, Faro, Portugal. (Oral communication)
94. Gavaia, PJ, Laizé, V, Witten, PE, Cancela, ML. 2008. Isolation and differentiation of mononucleated osteoclasts from the gilthead seabream (Sparus aurata), an in vitro study. 2nd Meeting
of the European Society for Evolutionary Development Biology, 29 July-1 August, Ghent, Belgium.
95. Goncalves, AF, Damasceno-Oliveira, A, Aluru, N, Vijayan, MM, Coimbra, J, Wilson, JM. 2008.
There is a cellular stress in response to hydrostatic pressure (3 MPa) acclimation in the shallow
water teleost Oncorhynchus mykiss. Society for Experimental Biology Annual Main Meeting, 6-10
July, Marseille, France. Comparative Biochemistry and Physiology Part A 150(3), Supplement 1:
S107.
96. Gonçalves, JFM, Damasceno-Oliveira, AP. 2008. Aquaculture project formulation. Proceedings of
the International Workshop: Developing a Sustainable Aquaculture Industry in the Azores, 2-5
June, Horta, Azores, Portugal. Arquipélago - Life and Marine Sciences, Supplement 7: 25-29.
97. Gonçalves, OM, Fontaínhas-Fernandes, A, Castro, LC, Smolka, A, Wilson, J. 2008. Gastric proton
pump expression in teleost fishes: nongastric sites of expression in gastric versus agastric fishes.
Society for Experimental Biology Annual Main Meeting, 6-10 July, Marseille, France. Comparative
Biochemistry and Physiology Part A 150(3), Supplement 1: S106.
98. Granja, H, Henriques, R, Macedo, JA, Alves, P, Honrado, J. 2008. An ecosystem approach to
vulnerability assessment in coastal dunes landscapes. International Dune Conference: Changing
Perspectives in Coastal Dune Management, Liverpool, UK.
99. Granja, H, Rocha, F, Matias, M, Caldas, F. 2008. Lagoa da Apúlia: a residual lagoon from Late
Holocene (NW coastal zone of Portugal). 5th International Geological Correlation Programme IGCP 495 Meeting - Quaternary Land-Ocean Interactions: Driving Mechanisms and Coastal
Responses. 27-29 October, Faro, Portugal.
100. Gravato, C, Almeida, J, Guilhermino, L. 2008. Molecular and biochemical effects of BaP are
associated with behavioural and growth alterations in sea bass. 29th Annual Meeting of the
Society of Environmental Toxicology and Chemistry - SETAC North America, 16-20 November,
Tampa, Florida, USA. Abstract book, p. 181.
101. Gravato, C, Araújo, M, Guilhermino, L. 2008. Determination of cholinesterase activity, oxidative
stress parameters, micronuclei and other nuclear abnormalities in the blood of yellow-legged
gulls, common buzzards and red-kites. 29th Annual Meeting of the Society of Environmental
Toxicology and Chemistry - SETAC North America, 16-20 November, Tampa, Florida, USA.
Abstract book, p. 289.
102. Gravato, C, Guilhermino, L. 2008. Is the accumulation of benzo(a)pyrene associated with the
swimming velocity inhibition of sea bass? CICTA 2008 - 7th Iberian and 4th Iberoamerican
Congress on Environmental Contamination and Toxicology, 10-12 March, Lisbon, Portugal.
103. Gravato, C, Van der Vem, K, Vandenbrouck, T, De Coen, W, Guilhermino, L. 2008. Linking gene
expression, biomarkers, behaviour and growth on sea bass chronically exposed to
benzo(a)pyrene. CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress on Environmental
Contamination and Toxicology, 10-12 March, Lisbon, Portugal. (Oral communication)
104. Gravato, C, Vandenbrouck, T, Van der Ven, K, De Coen, W, Guilhermino, L. 2008. Linking gene
expression, biomarkers and behaviour alterations induced by benzo(a)pyrene on sea bass. 18th
Annual Meeting of the Society of Environmental Toxicology and Chemistry - SETAC Europe, 25-29
May, Warsaw, Poland. Abstract book, p. 22. (Oral communication)
105. Guimarães, L, Afonso, MJ, Marques, MJ, Chaminé, HI, Guilhermino, L. 2008. Integrated quality
assessment of Porto spring galleries (NW Portugal). CICTA 2008 - 7th Iberian and 4th
Portugal.
106. Guimarães, L, Gravato, C, Borges, C, Guilhermino, L. 2008. Neurotoxic and oxidative stress
biomarkers responses in natural populations of Carcinus maenas from NW Portuguese estuaries
with different histories. 29th Annual Meeting of the Society of Environmental Toxicology and
Chemistry - SETAC North America, 16-20 November, Tampa, Florida, USA. Abstract book, p. 264.
107. Guimarães, L, Gravato, C, Guilhermino, L. 2008. Impact of pollution on the continental life-cycle
phase of the European eel: a comparative study in three Northern Portuguese estuaries. 18th
May, Warsaw, Poland. Abstract book, p. 175.
108. Guimarães, L, Gravato, C, Sousa, A, Guilhermino, L. 2008. Assessment of pollution in estuaries of
the NW coast of Portugal and its effects on wild populations of Pomatoschistus microps. 29th
Annual Meeting of the Society of Environmental Toxicology and Chemistry - SETAC North
America, 16-20 November, Tampa, Florida, USA. Abstract book, p. 264.
109. Guimarães, L, Santos, J, Gravato, C, Guilhermino, L. 2008. Effects of pollution on yellow eel’s
(Anguilla anguilla) development: a comparative study on three estuarine populations of the NW
coast of Portugal. Aquaculture Europe 2008, Session on European eel conservation and culture.
15-18 September, Krakow, Poland. Abstract book, p. 254-255. (Oral communication)
110. Haond, C, Canario, AVM. 2008. Re-examination of the immunolocalization of the cystic fibrosis
tansmembrane regulator in mouse kidney: evidence for its basolateral location in renal epithelial
cells. Takis Anagnostopoulos Symposium: Renal and Epithelial Physiology and Pathophysiology,
26-27 June, Paris, France. Nephron Physiology 110: 29.
111. Hermida, M, Costa, MJ, Maia, C, Reis, AR, Valente, A, Cruz, C, Saraiva, A. 2008. Occurrence of
Philometra ovata in the gudgeon Gobio lozanoi. I Peruvian Congress of Helminthology and
Associated Invertebrate - New approches about Neotropical Helminthology, 30 October-1
November, Lima, Peru.
112. Hernández-Moreno, D, Gravato, C, Perez-Lopéz, M, Soler-Rodriguez, F, Guilhermino, L. 2008.
Biochemical and behavioural effects of acute exposure to the pesticide carbofuran on the sea
bass (Dicentrarchus labrax). CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress on
Environmental Contamination and Toxicology, 10-12 March, Lisbon, Portugal.
113. Honrado, J, Macedo, JA, Vicente, J, Lomba, A, Henriques, RF, Granja, H, Barreto Caldas, F. 2008.
Community assembly in sand-dune vegetation as a source of indicators for vulnerability
assessment. 1st PoCoast Seminar on Coastal Research, 26-28 May, Porto, Portugal.
114. Howcroft, CF, Amorim, MJB, Gravato, C, Guilhermino, L, Soares, AMVM. 2008. Biomarkers in
Enchytraeus albidus (Oligochaeta): chemical and abiotic stress assessment. 18th Annual Meeting
of the Society of Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw,
Poland. Abstract book, p. 21. (Oral communication)
115. Howcroft, CF, Novais, S, Gravato, C, Amorim, MJB, Guilhermino, L, Soares, AMVM. 2008.
Cholinesterase activity in Enchytraeus albidus (Oligochaeta): in vivo and in vitro effects of
chemicals. 18th Annual Meeting of the Society of Environmental Toxicology and Chemistry SETAC Europe, 25-29 May, Warsaw, Poland. Abstract book, p. 120. (Oral communication)
116. Howcroft, CF, Gravato, C, Amorim, MJB, Guilhermino, L, Soares, AMVM. 2008. Effects of different
soil types, soil properties, betanal and copper on oxidative stress biomarkers of Enchytraeus
albidus. CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress on Environmental
Contamination and Toxicology, 10-12 March, Lisbon, Portugal.
117. Huertas, M, Canario, AVM, Hubbard, PC. 2008. Changes in olfactory sensitivity during the
European Eel (Anguilla Anguilla) life cycle. XV International Symposium on Olfaction and Taste,
21-26 July, San Francisco, USA. Chem. Senses 33: S150.
118. Jordanova, M, Rebok, K, Kostov, V, Rocha, E. 2008. Liver lesion in the barbel (Barbus
peloponnesius) from the river Bregalnica - Preliminary data along a suspected pollution gradient.
III International Symposium of Ecologists of the Republic of Montenegro - ISEM3, 8-12 October,
Herceg Novi, Montenegro.
119. Kijjoa, A, Sawangwong, P, Puchakarnand, S, Herz, W. 2008. Secondary metabolites from the
marine sponge Cliona patera. 1st Euro-Mediterranean Conference on Marine Natural Products EMCMNO-1, 31 October-4 November, Sharm El Sheikh, Egypt.
120. Kijjoa, A, Pinto, M, Gonzalez, MJ, Nascimento, MSJ, Mondranondra, I-O. 2008. Prenylated
xanthones and other constituents from the wood of Garcinia merguensis. 7th Joint Meeting of
AFERP, ASP, GA, PSE & SIF - Natural Products with Pharmaceutical, Nutraceutical, Cosmetic and
Agrochemical Interest, 3-8 August, Athens, Greece.
121. Kulhanek, DK, Voelker, AH, Grützner, J. 2008. Centennial-scale nannoplankton productivity
changes in the Mid-latitude North Atlantic during marine isotope stages 11-12: evidence from
IODP Site 1313. American Geophysical Union Fall Meeting 2008, 14-18 December, San Francisco,
USA. Abstract PP11B-1395.
122. Lago-Leston, MA, Agustí, S, Duarte, C, Serrão, EA, Pearson, G.A. 2008. Environmental
transcriptomics of southern ocean phytoplankton assemblies. The Annual International
Conference on Metagenomics, 3-7 November, La Jolla, San Diego, USA.
123. Laizé, V. 2008. FICEL flagship project: Functional genomics using marine fish cell lines and
embryos. Marine Genomics Final Assembly, 13-16 May, Faro, Portugal. (Oral communication)
124. Laizé, V, Viegas, CSB, Simes, DC, Cancela, ML. 2008. Evolutionary relationship of bone-associated
Gla proteins. 2nd Meeting of the European Society for Evolutionary Development Biology, 29
July-1 August, Ghent, Belgium.
125. Leite, RB, Afonso, R, Ascenso, R, Cancela, ML. 2008. Perkinsus sp. alternative oxidase:
Characterization and evolutionary considerations. 2nd Meeting of the European Society for
Evolutionary Development Biology, July 29 - August 1, Ghent, Belgium.
126. Leite, RB, Fonseca, L, Afonso, R, Simão, M, Ascenso, RM, Cancela, ML. 2008. Clam lectins: speciesspecific patterns of expression upon Perkinsus exposure and evolutionary considerations. Marine
Genomics Final Assembly, 13-16 May, Faro, Portugal.
127. Letão, A, Damasceno-Oliveira, A, Pereira, CM, Coimbra, JC, Wilson, JM. 2008. Transport stress in
glass eels. 6th AIEC Congress, Iberian Association of Comparative Endocrinology, 10-13
September, Cadiz, Spain. Avanços em Endocrinologia Comparativa, Vol. 4, pp. 63-68.
128. Lobo-da-Cunha, A, Oliveira, E, Coelho, R, Calado, G. 2008. The salivary glands in herbivorous and
carnivorous cephalaspidean opisthobranchs. 5th Congress of the European Malacological
Societies, 2-6 September, Ponta Delgada, Azores, Portugal. (Oral communication)
129. Lobo-da-Cunha, A. 2008. O peroxissoma: a investigação sobre um organelo celular. XVII Jornadas
Luso-Galaicas de Ciência e Desenvolvimento, 21-23 October, Santiago de Compostela, Spain.
130. Lopes, C, Abrantes, F, Mix, AC. 2008. Diatom transfer functions for sea surface temperature and
primary productivity in upwelling areas: the CUPEX project. American Geophysical Union Fall
Meeting 2008, 14-18 December, San Francisco, USA. Abstract PP41B-1459.
131. Luis, EA, Caldeira, RMA, Santos, AJP, Videman, J. 2008. Island wake dynamics: Madeira
Archipelago case study. 1st Workshop on Computational Engineering: Fluid Dynamics, 10-11 July,
Lisbon, Portugal. (Oral communication)
132. Macedo, JA, Silva, G, Lomba, A, Vicente, J, Henriques, R, Granja, H, Honrado, J. 2008.
Biogeographic bias and the need for functional approaches to bioindication in coastal areas. 1st
PoCoast Seminar on Coastal Research, 26-28 May, Porto, Portugal.
133. Machado, A, Abreu, C, Bordalo, AA. 2008. Structure, diversity and abundance of Archaea
populations along an estuarine salinity gradient (Douro estuary, Portugal). Symposium on
Current Issues and Perspectives in Aquatic Microbiology and Microbial Ecology. 25-29 May,
Wierzba, Poland. Abstract book, p. 67.
134. Machado, A, Magalhães, C, Mucha, A, Bordalo, AA. 2008. Denitrifier communities structure and
nitrous oxide potential rates in salt-marshes of two Portuguese estuaries. Symposium on Current
Issues and Perspectives in Aquatic Microbiology and Microbial Ecology, 25-29 May, Wierzba,
Poland. Abstract book, p. 65 (Oral communication)
135. Madureira, T, Rocha, MJ, Rocha, E, Cass, QB, Tiritan, ME. 2008. HPLC-DAD for screening of
pharmaceuticals from different therapeutic classes in Douro River estuary. 18th Annual Meeting
Poland.
136. Magalhães, C, Kiene, RP, Machado, A, Teixeira, C, Bordalo, AA. 2008. Novel inhibitory interaction
between DMSP degradation products and denitrification pathway. 12th International Symposium
on Microbial Ecology - ISME12, 17-22 August, Cairn, Australia.
137. Magalhães, C, Kiene, RP, Machado, A, Teixeira, C, Bordalo, AA. 2008. Inhibitory effects of DMSP
degradation products on denitrification in coastal marine sediments and in a bacterial culture.
12th International Symposium on Microbial Ecology - ISME12, 17-22 August, Cairn, Australia.
138. Magalhães, V, Buffett, B, Archer, D, Pinheiro, L. 2008. Gas hydrate stability in the Gulf of Cadiz,
potential and evolution. European Geosciences Union General Assembly, 13-18 April, Vienna,
Austria. Geophysical Research Abstracts, vol. 10, EGU2008-A-04097.
139. Magalhães, VH, Buffett, B, Archer, D, Pinheiro, LM. 2008. Effect of climate variations on gas
hydrate stability conditions at fluid escape structures in the Gulf of Cadiz. American Geophysical
Union Fall Meeting 2008, 14-18 December, San Francisco, USA. Abstract U23D-0090.
140. Marcos, R, Santos, M, Santos, N, Malhão, F, Monteiro, RAF, Rocha, E. 2008. Hepatocytes, hepatic
stellate cells and Kupffer cells: an unbiased estimation of their number using design-based
stereological methods. 14th International Symposium on Cells of the Hepatic Sinusoid, 31
October-4 September, Tromsø, Norway.
141. Marques, CL, Laizé, V, Planas, JV, Cancela, ML. 2008. Adipocytic transdifferentiation of gilthead
seabream bone-derived cell lines. Marine Genomics Final Assembly, 13-16 May, Faro, Portugal.
142. Marques, DKS, Amaral, CMC, Silva, EV, Casal, G, Matos, P, Matos, E, Azevedo, C. 2008.
Ultraestrutura do esporo de Henneguya sp. (Myxozoa) encontrado em pacu Piaractus
mesopotamicus, Pantanal, MS, Brasil. X ENBRAPOA, 17-20 November, Búzios, Brazil.
143. Martínez-Lopez, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, L, Guilhermino, L, GarcíaFernandez, AJ. 2008. Evaluation of exposure to heavy metals in Mediterranean spur-thighed
(Testudo graeca graeca) tortoise from Southeastern of Spain. 18th Annual Meeting of the Society
of Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw, Poland.
Abstract book, p. 73.
144. Martins, I, Silva, A, Santos, A, Bastos, L. 2008. Assessing critical runoff values for the generation
of a buoyancy induced coastal current off the western Iberian Peninsula. European Geosciences
Union General Assembly, 13-18 April, Vienna, Austria. Geophysical Research Abstracts, vol. 10,
EGU2008-A-10143.
145. Martins, M, Santos, ES, Barros, RJ, Costa, MC. 2008. Treatment of acid mine drainage with
sulphate-reducing bacteria using a two-stage bioremediation process. Proceedings of the 10th
International Mine Water Association Congress - IMWA`08, 2-5 June, Carlsbad, Czech Republic,
pp. 297-300.
146. Matos, E, Matos, P, Casal, G, Azevedo, C. 2008. A estrutura fina de Myxobolus sp. (Myxozoa)
parasita de Centromochlus sp. (n. v. Carataí) de Cametá (Pará), Brasil. XI Congresso LusoBrasileiro de Anatomia, 19-23 October, Belém, Pará, Brazil.
147. Matos, E, Matos, P, Casal, G, Azevedo, C. 2008. Aspectos preliminares ultraestruturais de
mixosporídio encontrado em peixe de água doce de Cametá: Hassar orestis. X ENBRAPOA, 17-20
November, Búzios, Brazil.
148. Matos, P, Casal, G, Matos, E, Azevedo, C. 2008. Aspectos preliminares ultraestruturais de
microsporídio encontrado em peixe de água doce: Brachyhypopomus (Mago-Leccia, 1994). X
ENBRAPOA, 17-20 November, Búzios, Brazil.
149. Matos, P, Casal, G, Matos, E, Azevedo, C. 2008. Ultraestrutura de Triangulamyxa sp. Parasita da
bexiga urinária de Colomesus psittacus (n. v. Baiacú) de Cametá (Pará), Brasil. XI Congresso LusoBrasileiro de Anatomia, 19-23 October, Belém, Pará, Brazil.
150. Matos, P, Fontaínhas-Fernandes, A, Galante, H, Monteiro, RAF, Rocha, E. 2008. Evaluating
organism-level consequences of neoplasia in fish using brown trout (Salmo trutta fario). 18th
May, Warsaw, Poland.
151. Matos, P, Fontaínhas-Fernandes, A, Galante, H, Monteiro, RAF, Rocha, E. 2008. Studying
functional consequences of necrosis in fish using tilapia nilotica (Oreochromis niloticus). 18th Annual
Warsaw, Poland.
152. Mendonça, I, Carvalho, ES, Silva, NCS, Negreiros, GO, Dias, LR, Casal, G, Matos, P, Matos, E,
Azevedo, C. 2008. Estrutura fina do esporo de Henneguya sp. parasita do teleósteo Hemiodopsis
sp. (Hemiodontidae) do rio Poty-Piauí. X ENBRAPOA, 17-20 November, Búzios, Brazil.
153. Mertens, K, Ribeiro, S, Bouimetarhan, I, Caner, H, Combourieu-Nebout, N, Dale, B, de Vernal, A,
Ellegaard, M, Filipova, M, Godhe, A, Groeneveld, J, Grøsfjeld, K, Gundersen, N, Holzwarth, U,
Kotthoff, U, Leroy, S, Londeix, L, Marret, F, Matsuoka, K, Mudie, P, Naudts, L, Peña-Manjarrez, JL,
Persson, A, Popescu, S, Pospelova, V, Sangiorgi, F, Van der Meer, M, Vink, A, Zonneveld, K,
Vercauteren, D, Vlassenbroeck, J, Louwye, S. 2008. Lingulodinium machaerophorum process
length variations in the surface sediment: a salinity proxy. 8th International Conference on
Modern and Fossil Dinoflagellates - Dino8, 4-10 May, Montreal, Canada.
154. Mertens, KN, Verhoeven, K, Verleye, T, Louwye, S, Amorim, A, Ribeiro, S, Deaf, AS, Harding, I, De
Schepper, S, Kodrans-nsiah, M, de Vernal, A, Radi, T, Dybkjaer, K, Poulsen, NE, Feist-burkhardt, S,
Chitolie, J, González Arango, C, Heilmann-clausen, C, Londeix, L, Turon, J-L, Marret, F,
Matthiessen, J, Mccarthy, F, Prasad, V, Pospelova, V, Hughes, JK, Riding, JB, Rochon, A, Sangiorgi,
F, Marino, G, Welters, N, Sinclair, N, Thun, C, Soliman, A, Van Nieuwenhove, N, Vink, A, Young, M.
2008. The absolute abundance calibration project: the Lycopodium marker-grain method put to
the test. 8th International Conference on Modern and Fossil Dinoflagellates - Dino8, 4-10 May,
Montreal, Canada.
155. Mil-Homens, M, Branco, V, Vale, C, Boer, W, Alt-Epping, U, Abrantes, F, Vicente, M. 2008. Record
of anthropogenic metal fluxes in the Tagus prodelta (Portugal). European Geosciences Union
General Assembly, 13-18 April, Vienna, Austria. Geophysical Research Abstracts, vol. 10,
EGU2008-A-08342.
156. Mil-Homens, M, Stevens, RL, Cato, I, Abrantes, F. 2008. Pb enrichements in short sediment cores
collected off the Portuguese Margin. XIV Seminário Ibérico de Química Marinha, 22-24
September, Cádiz, Spain.
157. Mira, S, dos Santos, A, Cancela, ML, Castro, M. 2008. Why does Norway lobster uses multiple
paternity? 8th Larval Biology Symposium, 6-11 July, Lisbon, Portugal.
158. Mira, S, Rainha, R, Cancela, ML. 2008. Identification of two microsatellites on the promoter of
Sparus aurata matrix Gla protein gene and their use on paternity analysis. Marine Genomics Final
Assembly, 13-16 May, Faro, Portugal.
159. Monteiro, SM, Rocha, E, Fontaínhas-Fernandes, A, Sousa, M. 2008. A stereological study of the
changes induced by copper on gills of Nile tilapia, Oreochromis niloticus. 18th Annual Meeting of
the Society of Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw,
Poland.
160. Morais, P, Sousa, R, Antunes, C, Solan, M. 2008. Influence of the Asian clam Corbicula fluminea
(Müller, 1774) in the Minho estuary ecosystem functioning: the effect of bioturbation. IV Iberian
Symposium of the River Minho Hydrological Basin, 14-15 November, Vila Nova de Cerveira,
Portugal.
161. Morais, P, Sousa, R, Antunes, C, Solan, M. 2008. Changes of ecosystem functioning in estuaries as
a consequence of non-indigenous invasive species introductions. Neobiota: 5th European
Conference on Biological Invasions, 23-26 September, Prague, Czech Republic.
162. Moreira-Silva, J, Coimbra, J, Wilson, J. 2008. Weatherloach (Misgurnus anguillicaudatus) actively
excretes ammonia through NHE. Society for Experimental Biology Annual Main Meeting, 6-10
July, Marseille, France. Comparative Biochemistry and Physiology Part A 150(3), Supplement 1:
S57. (Oral communication)
163. Moreira-Silva, J, Coimbra, J, Steffensen, J, Wilson, J. 2008. Effect of ammonia on weatherloach
(Misgurnus anguillicaudatus) metabolism under aquatic and aerial conditions. Society for
Experimental Biology Annual Main Meeting, 6-10 July, Marseille, France. Comparative
Biochemistry and Physiology Part A 150(3), Supplement 1: S107.
164. Nogueira, N, Andrade, C, Ozório, ROA. 2008. Effects of different dietary L-carnitine levels on
growth, feed utilization and body composition of red porgy (Pagrus pagrus) fingerlings. 13th
International Symposium on Nutrition and Feeding of Fish, 1-5 June, Florianopolis, Brazil.
165. Novais, S, Gravato, C, Amorim, M, Guilhermino, L, Soares, A, De Coen, W. 2008. Biomarker
responses in Enchytraeus albidus exposed to heavy-metals: effects at different times of
exposure. 5th World Congress of the Society of Environmental Toxicology and Chemistry, 3-7
August, Sydney, Australia.
166. Oliveira, E, Amorim, M, Gravato, C, Guilhermino, L, Soares, A. 2008. Bioaccumulation of
phenanthrene in Enchytraeus albidus. 5th World Congress of the Society of Environmental
Toxicology and Chemistry, 3-7 August, Sydney, Australia.
167. Osswald, J, Rellán, S, Gago-Martínez, A, Vasconcelos, V. 2008. Anatoxin-a studies: bioassays and
occurrence in Portuguese freshwaters. CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress
on Environmental Contamination and Toxicology, 10-12 March, Lisbon, Portugal.
168. Osswald, J, Rellán, S, Saker, M, Gago-Martínez, A, Vasconcelos, V. 2008. First detection of
anatoxin-a in human and animal dietary supplements containing cyanobacteria. 18th Annual
Warsaw, Poland.
169. Ozório, ROA, Gonçalves, R, Dias, S, Correia, AD, Gonçalves, J, Wilson, J. 2008. Protective effects of
dietary L-carnitine on turbot, Psetta maxima, challenged with S. parauberis and sublethal
ammonia concentration. 13th International Symposium on Nutrition and Feeding of Fish, 1-5
June, Florianopolis, Brazil.
170. Paiva, AP, Malik, P, Rosa, AM, Castro, C, Costa, MC. 2008. Extraction of metal ions from chloride
media: can malonamide derivatives be useful? Proceedings of the International Solvent
Extraction Conference - ISEC 2008, 15-19 September, Tucson, Arizona, USA. Moyer, BA (Ed), Vol.
2, pp. 1439-1444.
171. Páscoa, I, Fontaínhas-Fernandes, A, Wilson, J. 2008. Ammonia tolerance in the zebrafish (Danio
rerio): effects of ionic strength and ontogeny. Society for Experimental Biology Annual Main
Meeting, 6-10 July, Marseille, France. Comparative Biochemistry and Physiology Part A 150(3),
Supplement 1: S106.
172. Patton, GM, Martin, PA, Voelker, A. 2008. Mg/Ca Paleothermometry in the Central Gulf of Cadiz
during Heinrich events. American Geophysical Union Fall Meeting 2008, 14-18 December, San
Francisco, USA. Abstract PP11A-1368.
173. Pavanelli, GC, Eiras, JC, Takemoto, RM, Lacerda, ACF. 2008. Enfermidades de peixes de cultivo do
Brasil. III Simposio Internacional de Acuicultura, 15-18 September, Acuacuba, Havana, Cuba. (Oral
communication)
174. Pereira Silva, M, Sousa Pinto, I. 2008. Biodiversity and companies - Portugal, review on the
Portuguese business and biodiversity initiative. Biodiversity and Industry, BioStrat E-conference,
6-24 October.
175. Pereira Silva, M, Sousa Pinto, I. 2008. Biodiversity and companies - European overview. French
EPBRS Meeting - Biodiversity and Industry, 17-21 November, Paris, France. (Oral Communication)
176. Peres dos Santos, R, Brito, A, Cancela, ML, Gavaia, PJ. 2008. Developmental effects of warfarin in
the skeletogenesis of zebrafish (Danio rerio) larvae. Marine Genomics Europe Final Assembly, 1316 May, Faro, Portugal.
177. Pinheiro, P, Cardoso, J, Fuentes, J, Power, DM, Canário, AVM. 2008. A novel member and
comparative analysis of the parathyroid hormone family in chicken. 24th Conference of
European Comparative Endocrinologists - CECE, 2-6 September, Genoa, Italy.
178. Pinto, PIS, Teodósio, R, Socorro, S, Power, DM, Canario, AVM. 2008. Alternative transcripts for
sea bream (Sparus auratus) estrogen receptors. 6th Fish International Symposium on Fish
Endocrinology, 22-27 June, Calgary, Canada.
179. Prabhu, CN, Rodrigues, T, Flores, J-A, Voelker, A, Abrantes, F. 2008. Productivity response to the
SST and hydrography change in the Tagus prodelta during the Younger Dryas and the Holocene.
12th International Nannoplankton Association Meeting, 7-11 September, Lyon, France.
180. Prabhu, CN, Rodrigues, T, Flores, J-A, Voelker, A, Abrantes, F. 2008. The reconstruction of primary
productivity in the Portuguese-margin since the last glacial: Preliminary results from a multiproxy study. Symposium on Eastern Boundary Upwelling Ecosystems, 2-6 June, Las Palmas, Spain.
Abstract book, pp. 86-87.
181. Quartau, R, Trenhaile, AS, Mitchell, NC, Tempera, F. 2008. Modeling the origin and development
of a volcanic island shelf: Faial Island in the Azores archipelago. European Geosciences Union
General Assembly, 13-18 April, Vienna, Austria. Geophysical Research Abstracts, vol. 10,
EGU2008-A-04021.
182. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2008. Fish as an alternative model to explore bone
biology: role of four and a half LIM domains 2 in tissue mineralization. 35th European Symposium
on Calcified Tissues - ECTS, 24-28 May, Barcelona, Spain. Calcified Tissue International 82: S115.
183. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2008. The LIM-only protein FHL2 is associated with
cartilage development in fish. Marine Genomics Final Assembly, 13-16 May, Faro, Portugal.
184. Ramos, S, Ré, P, Bordalo, AA. 2008. Early life history of the Lima estuarine flatfish assemblages
(NW Iberian Peninsula). 7th International Flatfish Symposium, 2-7 November, Sesimbra, Portugal.
(Oral communication).
185. Ramos, S, Ré, P, Bordalo, AA. 2008. Unusual occurrence of Sardina pilchardus early life stages
within an estuarine system, Lima estuary NW Portugal. ECSA 2008 - Local Meeting on Climate
Change Impacts on South-European Coastal Ecosystems, 7-9 February, Lisbon, Portugal.
186. Rangel, MO, Gonçalves, JMS, Almeida, C, Afonso, C, Costa, C, Erzini, K, Oliveira, F, Monteiro, P,
Ribeiro, J, Veiga, P. 2008. Underwater eco-tourism routes - a case study in Central Algarve,
Portugal. Advances in Tourism Research 2008, 26-28 May, Aveiro, Portugal.
187. Rêgo, PC, Afonso, A, Monteiro, RAF, Rocha, E. 2008. Isolation liver macropheges from brown
trout (Salmo trutta fario) for toxicological testing of endocrone disruotors. 18th Annual Meeting
Poland.
188. Reis, PA, Antunes, C, Almeida, CM. 2008. Ecological quality of sediments from Minho estuary
saltmarsh - Assessment of metals bioavailability. SEASINK, 26-28 June, Porto, Portugal
189. Ribeiro, C, Tiritan, ME, Pardal, M, Viegas, I, Margalho, R, Rocha, E, Rocha, MJ. 2008. A balance of
the occurrence of selected estrogenic pollutants in the estuaries of the Rivers Douro, Mondego
and Sado, Portugal. 18th Annual Meeting of the Society of Environmental Toxicology and
Chemistry - SETAC Europe, 25-29 May, Warsaw, Poland.
190. Ribeiro, C, Urbatzka, R, Castro, F, Rocha, E, Carrola, JS, Fontaínhas-Fernandes, A, Tiritan, ME,
Rocha, MJ. 2008. Changes in the expression of genes encoding steroidogenic enzymes in the
gonads fromn the Nile tilapia (Oreochromis niloticus) exposed to several endocrine disruptors.
18th Annual Meeting of the Society of Environmental Toxicology and Chemistry - SETAC Europe,
25-29 May, Warsaw, Poland.
Rocha, MJ. 2008. Changes in the carbonyl/reductase 20-hydroxisteroid dehydrogenase
expression in the Nile tilapia (Oreochromis niloticus) exposed to several endocrine disruptors.
18th Annual Meeting of the Society of Environmental Toxicology and Chemistry - SETAC Europe,
25-29 May, Warsaw, Poland.
Rocha, MJ. 2008. Interference of endocrine disrupting compounds on the aromatase CYP19
expression in the Nile tilapia (Oreochromis niloticus). 18th Annual Meeting of the Society of
Environmental Toxicology and Chemistry - SETAC Europe, 25-29 May, Warsaw, Poland.
193. Ribeiro, S, Ellegaard, M, Abrantes, F, Amorim, A. 2008. Environmental changes in Western Iberia
over the last 200 years as evidenced by marine dinoflagellate cyst records. Symposium on
Eastern Boundary Upwelling Ecosystems, 2-6 June, Gran Canaria, Spain. Abstract book, pp. 70-71.
194. Ribeiro, S, Lundholm, N, Amorim, A, Ellegaard, M. 2008. Protoperidinium minutum from Portugal
(North-East Atlantic) - cyst-theca relationship and phylogeny as inferred from SSU and LSU rDNA
genetic sequences. 8th International Conference on Modern and Fossil Dinoflagellates - Dino8, 410 May, Montreal, Canada.
195. Ribeiro, S, Amorim, A. 2008. Dinoflagellate cyst assemblages from two contrasting sites in the
Azores (Portugal) - Horta Bay and D. João de Castro Seamount. 8th International Conference on
Modern and Fossil Dinoflagellates - Dino8, 4-10 May, Montreal, Canada.
196. Richard, N, Gavaia, PJ, Cordeiro, O, Silva, TS, Rodrigues, C, Rodrigues, PM, Conceição, LEC. 2008.
Dietary vitamin K supplementation and expression of proteins involved in skeleton development
of Senegalese sole (Solea senegalensis). XIII International Symposium on Fish Nutrition and
Feeding - XIII ISFNF, 1-5 June, Florianópolis, Brazil.
197. Rocha, ACS, Almeida, CMR, Mucha, AP, Vasconcelos, MTSD. 2008. Influence of Cu on the
exudation of organic acids by Halimione portulacoides. 4th European Bioremediation
Conference, 3-6 September, Crete, Greece.
198. Rocha, E. 2008. Are fish potential surrogate models for studying influences of estrogens and
peroxisome proliferator drugs upon liver peroxisomes and PPARs? The Fourth Aquatic Animal
Models of Human Disease Conference, The Nicholas School of the Environment Sciences and the
Duke Comprehensive Cancer Center, 31 January-3 February, Durham, North Carolina, USA.
(Invited Oral communication)
199. Rosa, JT, Cancela, ML, Laizé, V. 2008. Identification of a short-chain dehydrogenase/reductase in
fish associated with mineral formation. Marine Genomics Final Assembly, 13-16 May, Faro,
Portugal.
200. Ruiz, AM, Sousa, J, Hanssen, RF, Perski, Z, Gil, AJ, Bastos, L, Galindo-Zaldívar, J. 2008.
Measurement of ground subsidence in the Granada area (Southern Spain) using PS-InSAR.
International Congress on Geomatics and Surveying Engineering, 18-21 February, Valencia, Spain.
201. Salgado, MA, Fernandes, C, Teixeira, C, Cortez, A. 2008. PAHs and metals in a coastal lagoon
(Esmoriz/Paramos), Portugal. SEASINK, 26-28 June, Porto, Portugal. (Oral communication).
202. Salgueiro, E, Martin, PA, Voelker, A, Rodrigues, T, Abrantes, F, Grimalt, J. 2008. Upper water
column structure during Marine Isotope Stage 11.3 and the Holocene on the Portuguese Margin.
American Geophysical Union Fall Meeting 2008, 14-18 December, San Francisco, USA. Abstract
PP33C-1563.
203. Salgueiro, E, Martin, P, Abrantes, F. 2008. Temperature and productivity calibration along the
southern Portuguese margin, using modern planktonic foraminifera trace element and stable
isotope proxies. Symposium on Eastern Boundary Upwelling Ecosystems, 2-6 June, Las Palmas,
Spain. Abstract book, p. 89.
204. Samuel, N, Saveia, AP, Casal, G, Azevedo, C. 2008. Ultra-estrutura de oocisto de Nematopsis sp.
(Filo Apicomplexa) parasita de Arca senilis (Mollusca, Bivalvia, Arcidae) de Luanda, Angola. X
ENBRAPOA, 17-20 November, Búzios, Brazil.
205. Saraiva, A. 2008. Fish anisakids (Nematoda: Anisakidae) and their importance in public health. I
Peruvian Congress of Helminthology and Associated Invertebrate - New approches about
Neotropical Helminthology, 30 October-1 November, Lima, Peru. (Oral communication)
206. Serrão, ETA, Perrin, C, Billard, E, Pearson, GA, Destombe, C, Daguin, C, Valero, M. 2008. From
global to local scale of genetic structure within the species Fucus vesiculosus and F. spiralis. Algal
node satellite meeting, Marine Genomics Europe Final Assembly, 13-16 May, Faro, Portugal.
207. Silva, A, Martins, I, Bastos, L. 2008. An intermittent coastal current generated by buoyancy and
wind forcing. European Geosciences Union General Assembly, 13-18 April, Vienna, Austria.
Geophysical Research Abstracts, vol. 10, EGU2008-A-10209.
208. Silva, AJ, Martins, I, Bastos, L. 2008. Assessing the Existence of a Northwest Iberian Coastal
Current. Luso-Spanish Assembly of Geodesy and Geophysics, 11-14 February, Tomar, Portugal.
209. Silva, AJ, Martins, IM, Santos, AI, Moita, MT, Bastos, LC. 2008. Is there a Northwest Iberian
Coastal Current? PoCoast Seminar on Coastal Research, 26-28 May, Porto, Portugal.
210. Silva, P, Valente, LMP, Galante, MH, Timóteo, VMFA, Andrade, CAP, Monteiro, RAF, Rocha, E.
2008. Dietary protein content influences both growth and size distribution of anterior and
posterior muscle fibres of Pagellus bogaraveo juvelines. XIII International Symposium of Fish
Nutrition and Feeding, 1-5 June, Florianópolis, Brazil.
211. Simes, DC, Viegas, CSB, Williamson, MK, Price, PA, Cancela, ML. 2008. Purification of matrix Gla
protein and osteocalcin from the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish with
a cartilaginous endoskeleton. The International Bone and Mineral Society, 9-14 March, Davos,
Switzerland. Bone 42 (S1): S65.
212. Sousa, A, Guimarães, L, Guilhermino, L. 2008. Assessment of pollution in estuaries of the NW
coast of Portugal and its effects on wild populations of Pomatoschistus microps: what can
biomarkers add to chemical analysis? CICTA 2008 - 7th Iberian and 4th Iberoamerican Congress
on Environmental Contamination and Toxicology, 10-12 March, Lisbon, Portugal. (Oral
communication)
213. Sousa, JJ, Ruiz, AM, Hanssen, RF, Perski, Z, Bastos, L, Gil, AJ, Galindo-Zaldívar, J. 2008. Ps-InSAR
measurement of ground subsidence in Granada area (Betic cordillera, Spain). 13th FIG
Symposium on Deformation Measurements and Analysis and 4th IAG Symposium on Geodesy for
Geotechnical and Structural Engineering, 12-15 May, Lisbon, Portugal.
214. Sousa, JJ, Ruiz, AM, Hanssen, RF, Perski, Z, Bastos, L, Gil, AJ, Galindo-Zaldívar, J. 2008.
Monitorização de deformações na Bacia de Granada usando PS-InSAR. Luso-Spanish Assembly of
Geodesy and Geophysics, 11-14 February, Tomar, Portugal.
215. Sousa Pinto, I, Vieira, R. 2008. Monitoring intertidal biodiversity with schools. First World
Conference in Marine Biodiversity, 12-15 November, Valencia, Spain.
216. Sousa, R. 2008. Conservation and ecology in European tidal freshwater wetlands: opportunities
and challenges. Student Conference on Conservation Science 2008, University of Cambridge,
Cambridge, UK.
217. Sousa, R, Antunes, C, Guilhermino, L. 2008. Effects of the 2005 heatwave on mollusc community
of the River Minho estuary (NW Iberian Peninsula). 43rd Estuarine & Coastal Sciences Association
International Symposium, 7-9 February, Lisbon, Portugal. (Oral communication)
218. Sousa, R, Dias, S, Guilhermino, L, Antunes, C. 2008. Faunal biodiversity in the Minho estuary:
principal threats and possible management options. IV Iberian Symposium of the River Minho
Hydrological Basin, 14-15 November, Vila Nova de Cerveira, Portugal. (Oral communication)
219. Sousa, R, Nogueira, AJA, Antunes, C, Guilhermino, L. 2008. Extremely high production of the nonindigenous invasive species Corbicula fluminea: effects on ecosystem processes and functions.
Neobiota - 5th European Conference on Biological Invasions, Prague, Czech Republic. (Oral
communication)
220. Stoichev, T, Baptista, MS, Basto, MCP, Carvalho, PN, Vasconcelos, MTSD. 2008. Interactions of
alkylphenols with cyanobacterium Microcystis aeruginosa. 2nd EuCheMS Chemistry Congress,
16-20 September, Turin, Italy.
221. Teixeira, C, Magalhães, C, Joye, S, Bordalo, AA. 2008. Patterns of nitrate reduction processes in
estuarine sediments: an example from Southern Atlantic Europe. International Symposium on
Current Issues and Perspectives in Aquatic Microbiology and Microbial Ecology, 25-29 May,
Wierzba, Poland. (Oral communication)
222. Tiago, DM, Cancela, ML, Aureliano, M, Laizé, V. 2008. Insulin, IGF-1 and vanadate proliferative
and anti mineralogenic effects are mediated by MAPK and PI-3K pathways in fish cell lines.
Marine Genomics Final Assembly, 13-16 May, Faro, Portugal.
223. Tsui, TKN, Hung, CCY, Nawata, MC, Wilson, JM, Wright, PA, Wood, CM. 2008. In vitro evidence
for the involvement of Rh proteins and H+ efflux in ammonia excretion across the gills of the
freshwater rainbow trout. Society for Experimental Biology Annual Main Meeting, 6-10 July,
Marseille, France. Comparative Biochemistry and Physiology Part A 150(3), Supplement 1: S105.
224. Turini, BGS, Ozório, ROA, Gonçalves, JF. 2008. Protective effects of dietary l-carnitine on turbot
(Scophthalmus maximus) reared under different stocking densities. 13th International
Symposium on Nutrition and Feeding of Fish, 1-5 June, 2008, Florianopolis, Brazil.
225. Veiga, P, Ribeiro, J, Ditton, RB, Gonçalves, J, Erzini, K. 2008. Recreational rod and reel fishing from
the shore in the south of Portugal: a preliminary socio-economic approach. 5th World
Recreational Fishing Conference: The Angler in the Environment, 10-13 November, Dania Beach,
Florida, USA.
226. Viegas, M, Cancela, L, Conceição, N. 2008. Retinoic acid as an inhibitor of mineralization in a
Xenopus laevis bone derived cell line. 3rd International Meeting of the Portuguese Society for
Stem Cells and Cellular Therapy - SPCE-TC, 23-24 April, Faro, Portugal. (Oral communication)
227. Viegas, CSB, Simes, DC, Williamson, MK, Laizé, V, Price, PA, Cancela, ML. 2008. Identification of
an MGP/OC hybrid gene in the Adriatic sturgeon (Acipencer naccarii), an ancient bony fish with a
cartilaginous endoskeleton. 35th European Symposium on Calcified Tissues - ECTS, 24-28 May,
Barcelona, Spain. Calcified Tissue International 82: S121-122.
228. Vieira, LR, Nogueira, A, Morgado, F, Soares, AMVM, Guilhermino, L. 2008. Environmental
monitoring in a shallow lagoon using Pomatoschistus microps (Krøyer, 1838). 18th Annual
Warsaw, Poland. Abstract book, p. 177.
229. Vieira, LR, Nogueira, A, Morgado, F, Soares, AMVM, Guilhermino, L. 2008. Acute effects of copper
and mercury on Pomatoschistus microps (Krøyer, 1838). CICTA 2008 - 7th Iberian and 4th
Portugal.
230. Vieira, LR, Nogueira, AJA, Soares, AMVM, Morgado, F, Guilhermino, L. 2008. Biomonitoring
studies in populations of Pomatoschistus microps from a Portuguese shallow lagoon. CICTA 2008
- 7th Iberian and 4th Iberoamerican Congress on Environmental Contamination and Toxicology,
231. Vieira, MN, Bio, A, Peixoto, M. 2008. Assessment of biological water quality using aquatic benthic
macroinvertebrates. I Congreso Peruano de Helmintología e Invertebrados Afines: Nuevos
Enfoques en Helmintología Neotropical, 30 October-1 November, Lima, Peru.
232. Voelker, A, Salgueiro, E, de Abreu, L. 2008. Southward shift of export productivity maxima during
glacial cold periods off the western Iberian Margin. Symposium on Eastern Boundary Upwelling
Ecosystems, 2-6 June, Las Palmas, Spain. Abstract book, p. 73.
233. Voelker, AH, Martin, P, Lea, DW, Lebreiro, S. 2008. Tracing the influence of Mediterranean
outflow waters on the mid-depth Portuguese Margin between marine isotope stages 9 and 13.
American Geophysical Union Fall Meeting 2008, 14-18 December, San Francisco, USA. Abstract
PP33C-1566.
234. Weber, M, Ferreira, A. 2008. Natural conservation practices at the Littoral Station of Aguda,
Portugal. 7th International Aquarium Congress: Progress and Conservation, 19-24 October,
Shanghai, China. Abstract book, p. 104.
235. Wilairata, R, Pinto, M, Nascimento, MSJ, Kijjoa, A. 2008. Constituents of Schisandra verruculosa
and their cytotoxic effect on human cancer cell lines. International PSE Symposium on Natural
Products in Cancer Therapy, 23-26 September, Naples, Italy.
236. Wilson, JM, Choe, KP, Ip, YK. 2008. Remodeling of the fish gill in the climbing perch. 8th
International Congress on the Biology of Fishes, American Fisheries Society, 28 July-1 August,
Portland, USA. (Oral communication)
237. Wilson, JM, Laurent, P, Chevalier, C, Ip, YK, Lam, TJ, Sivalonganathan, S, Reddy, K. 2008. Does the
seahorse gill possess accessory cells? International Congress on the Biology of Fishes, American
Fisheries Society, 28 July-1 August, Portland, USA.
238. Wilson, JM, Reis-Santos, PN, McCormick, SD. 2008. Characterization of branchial ATPase activity
in lamprey (Petromyzon marinus l.) ammocoetes and transformers. International Congress on the
Biology of Fishes, American Fisheries Society, 28 July-1 August, Portland, USA.
239. Wilson, JM, Reis-Santos, PN, Taylor, JR, McCormick, SD, Grosell, M. 2008. ATPase expression gut
of the lamprey, Petromyzon marinus. Society for Experimental Biology Annual Main Meeting, 610 July, Marseille, France.
COMMUNICATIONS IN NATIONAL SCIENTIFIC MEETINGS
1.
Afonso, R, Leite, RB, Cancela, ML, Gama, FM. 2008. Studies on the biotechnological potential of
chitin produced by marine organisms. XVI Congresso Nacional de Bioquímica, 22-25 Outubro,
Ponta Delgada, Portugal.
2.
Almeida, AP, Marques, K, Pinto, M, Nascimento, MJ, Naengchomnong, W, Kijjoa, A. 2008.
Strophioblachia fimbricalyx Boerl (Euphorbiaceae): A plant with antitumoral profile in vitro. 1º
Encontro Nacional em Química Medicinal, 13-15 Novembro, Porto, Portugal.
3.
Almeida, J, Gravato, C, Guilhermino, L. 2008. Ecological relevance of benzo[a]pyrene acumulation
in seabass (Dicentrarchus labrax). 11º Encontro Nacional de Ecologia – Sustentabilidade do
Planeta Terra, 20-21 Novembro, Vila Real, Portugal. Livro de Resumos, p.41.
4.
Almeida, J, Gravato, C, Guilhermino, L. 2008. Efeitos do benzo(a)pireno em Dicentrarchus labrax:
acumulação, biomarcadores e danos oxidativos no cérebro e músculo. III Encontro Nacional de
Pós-Graduação em Ciências Biológicas, 3-5 Setembro, Faro, Portugal.
5.
Araújo, L, Cunha, M, Afonso, A. 2008. Senegalense sole (Solea senegalensis) cell and serum
inflammatory response after intraperitoneal infection with Tenacibaculum maritimum. III
Encontro Nacional de Pós-Graduação em Ciências Biológicas, 3-5 Setembro, Faro, Portugal.
6.
Azevedo, C. 2008. HAPLOSPORÍDIOS: Parasitas da fauna aquática. Congresso Nacional de
Doenças Infecciosas e Microbiologia Clínica, Sida e Parasitologia e XII Congresso Português de
Parasitologia, 8-11 Outubro, Vilamoura, Portugal. (Oral communication)
7.
Azevedo, C, Casal, G, Garcia, P, Matos, E. 2008. Anatomia ultrastrutural do esporo de
Chloromyxum sp. (Myxozoa, Myxosporea) encontrado na vesícula biliar da raia Rioraja agassizii
(Elasmobranchii, Rajidae). Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica,
Sida e Parasitologia e XII Congresso Português de Parasitologia, 8-11 Outubro, Vilamoura,
Portugal.
8.
Baptista, M, Almeida, CMR, Vasconcelos, V. 2008. Avaliação da ocorrência da neurotoxina β-Nmetilamino-L-alanina (BMAA) produzida por cianobactérias em águas doces e marinhas
portuguesas. 1º Encontro Português de Cianotoxinas, 7-8 Julho, Porto, Portugal. (Oral
communication)
9.
Caldeira, R. 2008. Dinâmica oceânica de mesoescala. Encontro Ciência em Portugal – Ciência
2008, 2-4 Julho, Lisbon, Portugal. (Oral communication)
10. Cardeira da Silva, J, Dionísio, G, Brito, AB, Cancela, ML, Gavaia, PJ. 2008. Effect of diet in skeleton
development of zebrafish (Danio rerio) larvae and juvenile fish. XVI Congresso Nacional de
Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
11. Cardoso, JCR, Coelho, N, Gomes, AS, Vieira, F, Power, DM. 2008. Evolution of the secretin
peptide-receptor systems in metazoan: co-evolution or serendipity. Congresso Nacional de
Bioquímica, 22-25 Novembro, Universidade dos Açores, São Miguel, Portugal.
12. Carvalho, P, Rodrigues, PNP, Basto, MCP, Vasconcelos, MTSD. 2008. Levels of butyltins in
sediment and suitability of salt marsh plants for rhizoremediation. I Encontro de Jovens
Investigadores da Universidade do Porto - IJUP08, 20-22 Fevereiro, Porto, Portugal. (Oral
communication)
13. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2008. Caracterização ultrastrutural e filogenética
de Pleistophora sp. (microsporidia) parasita de Brachyhypopomus sp. do rio amazonas.
Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica, Sida e Parasitologia e XII
Congresso Português de Parasitologia, 8-11 Outubro, Vilamoura, Portugal. (Oral communication)
14. Casal, G, Matos, E, Teles-Grilo, ML, Azevedo, C. 2008. Dados ultrastruturais e moleculares de uma
microsporidiose diagnosticada no peixe teleósteo, Gymnorhamphichthys rondoni da fauna
amazónica. Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica, Sida e
Parasitologia e XII Congresso Português de Parasitologia, 8-11 Outubro, Vilamoura, Portugal.
15. Cerca, F, Andrade, F, Hinzmann, M, Ribeiro, A, Sampaio, P, Lobo-da-Cunha, A, Azeredo, J,
Vilanova, M. 2008. Staphylococcus epidermidis Biofilm: an antibiotic resistant subpopulation
defined. INCOMAM´08, International Conference on Microscopy and Microanalysis - XLIII
Reunião Anual da Sociedade Portuguesa de Microscopia, 30-31 Outubro, Porto, Portugal. (Oral
communication)
16. Cidade, H, Silva, CC, Oliveira, AE, Almeida, AP, Silva, AMS, Naengchomnong, W, Pinto, M, Kijjoa,
A, Nascimento, MSJ. 2008. Search for antitumor agents from Agrostistachys gauichaudii
(Euphorbiaceae). 1º Encontro Nacional em Química Medicinal, 13-15 de Novembro, Porto,
Portugal.
17. Coimbra, J. 2008. CIMAR – Laboratório Associado. Encontro Ciência em Portugal – Ciência 2008,
2-4 Julho, Lisboa, Portugal. (Oral communication)
18. Coimbra, J, Moreira, SM. 2008. Oceano 2020, uma agenda para a Universidade do Porto. 3º
Encontro Porto-Cidade-Região, 28-29 Janeiro, Porto, Portugal. (Oral communication)
19. Conceição, L. 2008. Sustentabilidade da aquacultura no sul da Europa: projecto SEACASE.
Encontro Ciência em Portugal – Ciência 2008, 2-4 Julho, Lisboa, Portugal. (Oral communication)
20. Conceição, N, Cox, C, Simões, B, Viegas, M, Cancela, ML. 2008. Computational identification of
regulatory motifs in cartilage-expressed genes using zebrafish as an in vivo model. XVI Congresso
Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
21. Costas, B, Aragão, C, Míguez, JM, Soengas, JL, Conceição, LEC. 2008. Effects of amino acids
supplementation on stress response and dopaminergic and serotonergic activities in Solea
senegalensis. III Encontro Nacional de Pós-Graduação em Ciências Biológicas, 3-5 Setembro, Faro,
22. Cruzeiro, L. 2008. Exploring protein's multi-funnel energy landscape. 1º Fórum Português em
Biologia Computacional, 10-12 Junho, Instituto Gulbenkian de Ciência, Oeiras, Portugal.
23. Dias, AC, Almeida, CMR, Mucha, AP, Vasconcelos, MTSD. 2008. Influence of organic pollutants on
the accumulation of copper by a salt marsh plant. I Encontro de Jovens Investigadores da
Universidade do Porto - IJUP08, 20-22 Fevereiro, Porto, Portugal.
24. Fazenda, C, Conceição, N, Cancela, ML. 2008. Possible regulation of the matrix Gla protein gene
by the transcription factor MEF2c. XVI Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta
Delgada, Portugal.
25. Gonçalves, JMS. 2008. Impactos da pesca comercial: o caso das rejeições. III Encontro de Pósgraduação em Ciências Biológicas, 3-5 Setembro, Faro, Portugal.
26. Gravato, C. 2008. Efeitos de produtos derivados do petróleo em peixe: genes, biomarcadores e
comportamento. Encontro Ciência em Portugal – Ciência 2008, 2-4 Julho, Lisboa, Portugal. (Oral
communication)
27. Guilhermino, L. 2008. Avaliação de risco ecológico: uma nova oportunidade para Biólogos.
Bioeconomia - III Congresso da Ordem dos Biólogos, 25-27 Fevereiro, Lisboa, Portugal. (Oral
communication)
28. Guilhermino, L. 2008. Relevância ecológica de estudos de monitorização a longo prazo: a
importância da integração de diferentes abordagens. 11º Encontro Nacional de Ecologia –
Sustentabilidade do Planeta Terra, 20-21 Novembro, Vila Real, Portugal. Livro de Resumos, p. 62.
29. Guilhermino, L. 2008. Riscos ecológicos em zonas estuarinas. I Ciclo de Workshops
Especializados. Sessão 4: Integração do Litoral Norte, Incluindo os Recursos do Mar nos PGBH.
Administração da Região Hidrográfica do Norte & Faculdade de Engenharia do Porto, 29
Setembro, Porto, Portugal. (Oral communication)
30. Guimarães, L, Gravato, C, Santos, J, Guilhermino, L. 2008. Impact of pollution on the continental
life-cycle phase of eel (Anguilla anguilla) populations from the estuaries of Minho, Lima and
Douro rivers. 11º Encontro Nacional de Ecologia – Sustentabilidade do Planeta Terra, 20-21
Novembro, Vila Real, Portugal. Livro de Resumos, p.71.
31. Howcroft, C, Amorim, MJB, Gravato, C, Guilhermino, L, Soares, AMVM. 2008. The use of
biomarkers in Enchytraeus albidus for chemical and abiotic stress assessment and the linkage to
traditional endpoints. III Encontro Nacional de Pós-Graduação em Ciências Biológicas, 3-5
Setembro, Faro, Portugal. (Oral communication)
32. Kijjoa, A, Nazareth, N, Nascimento, MSJ, Cordeiro da Silva, A, Wattanadilok, R. 2008. Assaying in
vitro the effects of kuanoniamine C on the growth of ER (+) and ER(-) human breast cancer cell
lines (P018). 1º Encontro Nacional em Química Medicinal, 13-15 Novembro, Porto, Portugal.
33. Leite, RB, Afonso, R, Cancela, ML. 2008. The presence of two alternative oxidases in Perkinsus
sp.: Characterization, expression patterns and evolutionary considerations. XVI Congresso
Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal. (Comunicação oral)
34. Lobo-da-Cunha, A, Ferreira, I, Calado, G. 2008. Microscopical characterization of the salivary
glands of Philinopsis depicta (Mollusca). INCOMAM´08, International Conference on Microscopy
and Microanalysis - XLIII Reunião Anual da Sociedade Portuguesa de Microscopia, 30-31 Outubro,
Porto, Portugal.
35. Madeira, C, Mira, S, Cabrita, E, Soares, F, Cancela, ML. 2008. Contribuição Parental do linguado
do Senegal (Solea senegalensis) em Aquacultura. III Encontro Nacional de P s- raduação em
Ciências iol gicas, 3-5 Setembro, Faro Portugal. (Oral communication)
36. Marques, CL, Laizé, V, Cancela, ML. 2008. Transcriptional regulation of seabream BMP2 gene. XVI
Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
37. Martins da Costa, P. 2008. Poultry sanitary inspection; anatomic and physiological perspectives.
IV Congresso da Sociedade Portuguesa de Ciências Veterinárias e I Congresso Ibérico de
Epidemiologia, 27-29 Setembro, Santarém, Portugal.
38. Matos, A, Borges, M-T, Castro, PML. 2008. Microbial community distribution and dynamics in a
shallow raceway recirculating mariculture system. I Encontro de Jovens Investigadores da
Universidade do Porto - IJUP08, 20-22 Fevereiro, Porto, Portugal. Livro de Resumos, p.193.
39. Osswald, J, Rellán, S, Gago, A, Vasconcelos, V. 2008. Produção de anatoxina-a por estirpes
cianobacterianas portuguesas. 1º Encontro Português de Cianotoxinas, 7-8 Julho, Porto, Portugal.
40. Osswald, J, Guilhermino, L. 2008. Efeitos da anatoxina-a na carpa comum (Cyprinus carpio L.) a
diferentes temperaturas. Respostas genéticas e ecologicamente relevantes. 11º Encontro
Nacional de Ecologia – Sustentabilidade do Planeta Terra, 20-21 Novembro, Vila Real, Portugal.
Livro de Resumos, p.78.
41. Parameswaran, V, Laizé, V, Cancela, ML. 2008. Pluripotency capacity of ESSA1, a seabream
blastula-derived stem cell culture. XVI Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta
Delgada, Portugal.
42. Peixe, C, Pereira, CM, Silva, F, Borges, M-T. 2008. Sensor for amperometric determination of
ammonia in seawater. I Encontro de Jovens Investigadores da Universidade do Porto - IJUP08,
20-22 Fevereiro, Porto, Portugal. Livro de Resumos, p.127.
43. Pereira, J, Baptista, MS, Stoichev, T, Heggie, B, Vasconcelos, VM, Vasconcelos, MTSD. 2008.
Survey of the effects of the antibiotic minocycline on freshwater cyanobacteria. I Encontro de
Jovens Investigadores da Universidade do Porto - IJUP08, 20-22 Fevereiro, Porto, Portugal.
44. Peres dos Santos, R, Brito, AB, Dionísio, G, Gavaia, PJ, Cancela, ML. 2008. Warfarin effects on
zebrafish (Danio rerio) skeletal development: in vivo inhibition of mineralization-related Gla
proteins activity. XVI Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
45. Poeta, P, Radhouani, H, Gonçalves, A, Carvalho, C, Martins da Costa, P, Rodrigues, J, Torres, C,
Igrejas, G. 2008. Caracterização Molecular de β-lactamases de largo espectro em Escherichia coli
de Animais Selvagens em Portugal. IV Congresso da Sociedade Portuguesa de Ciências
Veterinárias e I Congresso Ibérico de Epidemiologia, 27-29 Setembro, Santarém, Portugal.
46. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2008. Bone-related function of the LIM-only protein
FHL2. XVI Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
47. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Franquinho, F, Monteiro, RAF, Rocha, E. 2008.
Histological and stereological characterization of brown trout (Salmo trutta) trunk kidney.
INCOMAM´08, International Conference on Microscopy and Microanalysis - XLIII Reunião Anual
da Sociedade Portuguesa de Microscopia, 30-31 Outubro, Porto, Portugal. (Oral communication)
48. Rodrigues, PNR, Carvalho, PN, Alves, F, Evangelista, R, Basto, MCP, Vasconcelos, MTSD. 2008. An
expeditious method for determination of residues of organochlorine pesticides in sediments and
its application to Portuguese estuarine areas. I Encontro de Jovens Investigadores da
Universidade do Porto - IJUP08, 20-22 Fevereiro, Porto, Portugal.
49. Saraiva, A. 2008. Metacestodes da Família Gryporhynchydae (Cestoda: Cyclophyllidea) parasitas
de peixes. Congresso Nacional de Doenças Infecciosas e Microbiologia Clínica, Sida e
Parasitologia e XII Congresso Português de Parasitologia, 8-11 Outubro, Vilamoura, Portugal.
50. Simões, B, Conceição, N, Cancela, ML, Kelsh, R. 2008. Runx3 structure and function in zebrafish
neural crest: expression pattern and morpholino knockdown. Seminários Mar e Ambiente, 8
Maio, Faro, Portugal
51. Simões, B, Rodrigues, D, Conceição, N, Kelsh, R, Cancela, ML. 2008. Spatial-temporal expression
of Runx3 during early zebrafish development and in silico and in vitro analysis of its promoter
regions. XVI Congresso Nacional de Bioquímica, 22-25 Outubro, Ponta Delgada, Portugal.
52. Sousa, R, Antunes, C, Guilhermino, L. 2008. Distribution and functional role of bivalves present in
the river Minho tidal freshwater wetlands. 11º Encontro Nacional de Ecologia – Sustentabilidade
do Planeta Terra, 20-21 Novembro, Vila Real, Portugal. Livro de Resumos, p.55.
53. Sousa, R, Antunes, C, Guilhermino, L. 2008. The functional importance of the Asian clam
Corbicula fluminea in the Minho estuary. III Encontro Nacional de Pós-Graduação em Ciências
Biológicas, 3-5 Setembro, Faro, Portugal. (Oral communication)
54. Vasconcelos, V. 2008. Riscos ambientais e de saúde pública associados a cianobactérias tóxicas.
Encontro Ciência em Portugal – Ciência 2008, 2-4 Julho, Lisboa, Portugal. (Oral communication)
55. Vieira, LR, Nogueira, AJA, Soares, AMVM, Morgado, F, Guilhermino, L. 2008. Environmental PAH
pollution biomonitoring studies in a Portuguese Shallow Lagoon using Pomatoschistus microps
(Krøyer, 1838) as sentinel species. III Encontro Nacional de Pós-Graduação em Ciências
Biológicas, 3-5 Setembro, Faro, Portugal.
56. Vieira, LR, Nogueira, AJA, Soares, AMVM, Morgado, F, Guilhermino, L. 2008. Estudo ecológico e
ecotoxicológico numa lagoa costeira: abordagem integrada e análise multivariada. 11º Encontro
Nacional de Ecologia – Sustentabilidade do Planeta Terra, 20-21 Novembro, Vila Real, Portugal.
Livro de Resumos, p.88.
D.
PATENTS
1.
Cancela, ML, Brito, AB, Gavaia, PJ, Afonso, N, Laizé, V. 2008. U.S. Patent: US 61/105.975. No
confirmation: 8119. Date of deposit: 16 October 2008.
2.
Correia, M, Cunha, S. 2008. Portable equipment for measuring and calculating locomotion
dynamic parameters, National patent request no. 103933.
3.
Costa, MC, Martins, M, Barros, R. 2008. Uso de resíduos vinícolas como fonte de carbono e de
energia para bactérias sulfato-redutoras. Portuguese Patent nº 104182.
4.
Cruzeiro, L. 2008. Acceleration of catalysed chemical reactions. Portuguese Provisional Patent
Application no. 104302 K.
5.
Simes, DC, Viegas, CSB, Cancela, ML. 2008. US Patent: US 61/136.315. Date of deposit: 27 August
2008.
Information compiled by:
Susana Moreira
CIIMAR Science Office
Rua dos Bragas 289
4050-123 Porto
Portugal
Phone: +351 22 339 06 07
E-mail: [email protected]
Please use the above contact for data improvement.

Evaluation Report - 2008 CIMAR_Evaluation_Report_2008

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