Title Induction of Callus from Mistletoe and Interaction with its Host

Transcrição

Title Induction of Callus from Mistletoe and Interaction with its Host
Title
Author(s)
Citation
Issue Date
URL
Induction of Callus from Mistletoe and Interaction with its
Host Cells
Fukui, Mitsue; Azuma, Jun-Ichi; Okamura, Keizo
京都大学農学部演習林報告 = BULLETIN OF THE KYOTO
UNIVERSITY FORESTS (1990), 62: 261-269
1990-12-20
http://hdl.handle.net/2433/191963
Right
Type
Textversion
Departmental Bulletin Paper
publisher
Kyoto University
2
6
1
I
n
d
u
c
t
i
o
no
fCaH
耶 f I 加E 路 s
t
l
e
t
o
e
a
n
dI
n
t
e
r
a
c
t
i
o
nw
i
t
hi
t
sH
o
s
tC
e
l
l
s
MitsueFUKUI,JUル i
c
h
iA
z
UMA,and K
eizo OKAMURA
ヤドリギ@カルスの誘導と
宿主細胞との栴互作用
福井充枝・東
1
員
一岡村
Resume
A 回l1u
s was i
n
d
u
c
e
d from m
i
s
t
l
e
t
o
e (Viscum album L
.v
a
rl
u
t
e
s
c
e
n
s M.), a
nd
e
s
t
a
b
l
i
s
h
e
d on a m
o
d
i
f
i
e
d Murashig
e
Skoog (MS) medium h
a
v
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ga h
a
l
fo
ft
h
ei
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a
l MS medium and s
u
p
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m
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h1
.0 ppm o
fk
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. When t
h
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al
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o
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0
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要
ヤドリギ (
Viscumαlbum L
.v
a
rl
u
t
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s
c
e
n
s M.) から、カイネチン1.0ppm,ナフタレン酢費量
1
0
.
0
p
p
mを含む,イオン強度会半分にした M
u
r
a
s
h
i
g
e
S
k
o
o
g(MS) 改変培地を用い, 2
80C、 1
2
時間の明暗府知,光度約 2
,
5
0
0
1
u
xの条件下で、培謎し,カルスを誘導した。カルスは淡いピンク色
1
で、あった。また,プランテックスを用いて, 3週間措援した
で,その細胞の平均間後は約7.5μm
とき,その成長識が最大ζ
l述 し , 絶 乾 畿 が 約 3
{
告になった カルス細胞には,分子・議 7
7,
0
0
0、
1
9,
4
0
0のガラクトース特異性レクチンが見出されたが,これらは,ヤドリギの諜にも問機に見 1
1
¥
O
された。一方、ヤドリギのカルスをブナのカルスに接触させたところ,拘カルスは接触 i
院議を起
さずに生長し統ける乙とがわかった。
1
.I
NTRODUCTION
P
l
a
n
tc
巴l
l
sa
r
e known t
or
e
c
o
g
n
i
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ee
a
c
ho
t
h
e
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ym
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t
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a
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o
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t and p
r
o
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e
c
tt
h
e
m
s
e
l
v
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a
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n
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te
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r
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r
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n
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o
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o
n
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e
y
a
n
c
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fi
n
f
o
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m
a
t
i
o
n
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e
yt
oc
l
a
r
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e
c
o
g
n
i
t
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y
s
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e
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se
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p
e
c
t
e
dt
ob
eo
b
t
a
i
n
e
d from
2
6
2
a
r
a
s
i
t
ei
n
t
e
r
a
c
t
i
o
n
s
. 1
nt
h
ef
i
e
l
do
f woody p
l
a
n
t,
c
h
a
r
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c
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e
r
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ft
h
ep
l
a
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th
o
s
tp
m
i
s
t
l
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t
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eand i
t
sh
o
s
ts
田 町1 t
o be an a
t
t
r
a
c
t
i
v
es
t
a
r
t
i
n
gm
a
t
e
r
i
a
l
sf
o
rt
h
i
sl
i
n
eo
f
i
n
v
e
s
t
i
g
a
t
i
o
n
. Althought
h
ep
h
y
s
i
o
l
o
g
i
c
a
l
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ya
c
t
i
v
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n
s
t
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e
n
t
so
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i
s
t
l
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o
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t
sn
u
t
r
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e
n
t
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t
l
ei
s
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a
t
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o
n
s toward h
o
s
t and anatomy o
fm
i
s
t
l
e
t
o
eand h
o
s
t were r
e
p
o
r
t
e
dH 2), l
knowna
b
o
u
tc
e
l
l
c
e
l
li
n
t
e
r
a
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t
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o
nbetw配 nm
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s
t
l
e
t
o
eandi
t
sh
o
st.官時間f
o
r
e,i
nt
h
i
ss
t
u
d
y
wei
n
t
e
n
dt
oi
n
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et
h
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e
t
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nm
i
s
t
l
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t
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e(Viscumαlbum L
.
)andb
e
e
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h
(Fagus c
r
e
間 t
a Bl
.
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nv
i
t
r
o
. F
i
r
s
t,we t
r
i
e
dt
oi
n
d
u
c
ea c
a
l
l
u
s from m
i
s
t
l
e
t
o
e and
c
o
n
f
i
r
m
e
dt
h
ep
r
e
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e
n
s
eo
ft
h
esamek
i
n
do
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a
l
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e
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gp
r
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e
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e
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t
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a
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e
l electrophoresis.τhen t
h
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r
a
s
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t
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l
l
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蹴 ha
s
werec
o
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t
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tc
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l
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eon t
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to
fb
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grown i
nt
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.
附
2
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2
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l
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V
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y
M
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o
r
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i
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a
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n May,1
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幽
4
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