Curriculum Vitae

Curriculum Vitae
Identification
Full Name: Miguel Ângelo Rodrigues Larguinho
National Identity Card: 12609456
Birth place: Almada
Birth date: 17/09/1984
Nationality: Portuguese
Marital status: Single
Living Address: Rua Miguel Russel, Nº16-1ºB, Quinta da Marialva, 2855-120 CORROIOS
Cell phone: +351 964 978 741
E-mail: [email protected]
Driving License: SE-209180 7
Academic Degree
Title: Molecular and Cellular Biology
Graduation Year: 2007
Classification: 16 out of 20
Academic Degree: Licenciatura (4-year degree)
Institution: Faculty of Science and Technology/University NOVA de Lisboa
Scientific and professional activities
Period
Category
Institution
April 2010 - ongoing
PhD student
CIGMH/Life Science Dept. and REQUIMTE/ Chemistry
Dept., Faculty of Science and Technology/University
NOVA de Lisboa
September 2009 – March
Research
CIGMH/Life Science Dept., Faculty of Science and
2010
assistant
Technology/University NOVA de Lisboa
July 2008 – July 2009
Research
CIGMH/Life Science Dept., Faculty of Science and
assistant
Technology/University NOVA de Lisboa
Research interests
Area of scientific activity: Bionanotechnology, nanodiagnostics, toxicology, HPLC, MALDITOF-MS.
Fields of specialisation: Bionanotechnology, gold nanoparticles, detection of biological
molecules, nanodiagnostics, DNA adducts, MALDI-TOF-MS, toxicology.
Present research interests: Bionanotechnology, detection of biological molecules,
nanodiagnostics, biosensors, therapy, nanotoxicology, genetic and aquatic toxicology, cell
biology and genetics, mass spectrometry.
Publications
Scientific Papers
M. Larguinho, S. Santos, J. Almeida, P.V. Baptista (2014) DNA adduct identification using
gold-aptamer nanoprobes. To be Submitted.
M. Larguinho, D. Correia, M.S. Diniz, P.V. Baptista (2014) Evidence of one-way flow
bioaccumulation of gold nanoparticles across two trophic levels. Journal of Nanoparticle
Research. Submitted for revision.
D.V. Luis, J. Silva, A.I. Tomaz, R.F.M. de Almeida, M. Larguinho, P.V. Baptista, L.M.D.R.S.
Martins, T.F.S. Silva, P.M. Borralho, C.M.P. Rodrigues, A.S. Rodrigues, A.J.L. Pombeiro, A.R.
Fernandes. Insights into the mechanisms underlying the anti-proliferative potential of a Co(II)
coordination compound bearing 1,10-phenanthroline-5,6-dione: DNA and protein interaction
studies. Journal of Biological Inorganic Chemistry. Just Accepted.
M. Larguinho, P.M. Costa, G. Sousa, M.H. Costa, M.S. Diniz, P.V. Baptista (2014)
Histopathological findings on Carassius auratus hepatopancreas upon exposure to acrylamide:
correlation with genotoxicity and metabolic alterations. Journal of Applied Toxicology
(doi:10.1002/jat.2936).
J. Conde, M. Larguinho, A. Cordeiro, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R.
Fernandes, P.V. Baptista (2014) Gold-nanobeacons for gene therapy: evaluation of
genotoxicity, cell toxicity and proteome profiling analysis. Nanotoxicology 8:521-532
(doi:10.3109/17435390.2013.802821).
M. Larguinho, J.L. Capelo, P.V. Baptista (2013) Fast nucleotide identification through
fingerprinting using gold nanoparticle-based surface-assisted laser desorption/ionisation.
Talanta 105:417-421 (doi:10.1016/j.talanta.2012.10.072).
M. Larguinho and P.V. Baptista (2012) Gold and silver nanoparticles for clinical diagnostics From
genomics
to
proteomics.
Journal
of
Proteomics
75:2811-2823
(doi:10.1016/j.jprot.2011.11.007).
C. Nuñez, J. Fernandez-Lodeiro, M.S. Diniz, M. Larguinho, J.L. Capelo, C. Lodeiro (2011)
Synthesis and photophysical studies of two luminescent chemosensors based on cathecol and 8Hydroxyquinolone chromophores, and their complexes with group 13 metal ions. Inorganic
Chemistry Communications 14:831-835 (doi:10.1016/j.inoche.2011.02.023).
G. Doria, M. Larguinho, J.T. Dias, E. Pereira, R. Franco, P.V. Baptista (2010) Gold-silver alloy
nanoprobes
for
one-pot
multiplex
DNA
detection.
Nanotechnology
21:255101
(doi:10.1088/0957-4484/21/25/255101).
M. Larguinho, H.M. Santos, G. Doria, H. Scholz, P.V. Baptista, J.L. Capelo (2010)
Development of a fast and efficient ultrasonic-based strategy for DNA fragmentation. Talanta
81:881-886 (doi:10.1016/j.talanta.2010.01.032).
Book chapters
M. Larguinho, S. Figueiredo, A. Cordeiro, F.F. Carlos, M. Cordeiro, P. Pedrosa, P.V. Baptista
(2014) Nanoparticles for diagnostic and imaging. Frontiers in Nanomedicine – vol I. Bentham
Science. To be Submitted.
M. Larguinho, J.L. Capelo, P.V. Baptista (2013) Nanoparticles for mass spectrometry
applications. Handbook of Nanoparticles, Synthesis, Functionalization and Surface Treatment.
Springer-Verlag GmbH, Germany. Submitted for consideration.
Communications
Oral communications
G. Doria, J. Conde, M. Larguinho, P. Baptista (2010) Multiplex genetic characterization via
noble metal nanoprobes. Trends in NanoTechnology 2010. 6th-10th September 2010. Braga,
Portugal (oral presentation by GD).
G. Doria, M. Larguinho, J.T. Dias, E. Pereira, R. Franco, P. Baptista. AuAg-alloy-nanoprobes
for specific nucleic acid detection. Nanotech Conference & Expo 2010. June 21st-24th 2010.
Anaheim, USA (oral presentation by GD).
M. Larguinho. Bionanotechnology for nanodiagnostics – the emergence of SALDI-TOF for
biological analysis. XI Jornadas de Biologia Aplicada. 28th-30th April 2010. Braga, Portugal
(oral presentation by ML).
Posters in conferences
M. Larguinho, A. Cordeiro, P.M. Costa, M.S. Diniz, P.V. Baptista (2013) Histopathologic
effects of waterborne acrylamide in Mytilus galloprovincialis. Microscopy in Research 2013.
9th-10th December 2013. Caparica, Portugal.
J. Conde, M. Larguinho, A. Cordeiro, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R.
Fernandes, P.V. Baptista (2013) Gold-Nanobeacons for gene silencing: evaluation of the effect
and toxicity of a new nanotheranostics tool. European Human Genetics Conference 2013. 8th11th June 2013. Paris, France.
M. Larguinho, D. Correia, M.S. Diniz, P.V. Baptista (2013) Gold nanoparticle bioaccumulation
and nanotoxicity in marine environments. II National Encounter in Nanotoxicology. 2nd-3rd
April 2013. Lisbon, Portugal.
A. Cordeiro, M. Larguinho, J. Conde, L.R. Raposo, P.M. Costa, S. Santos, M.S. Diniz, A.R.
Fernandes, P.V. Baptista (2013) Analysing the toxicological potential of an antisense Aunanobeacon system for gene therapy. II National Encounter in Nanotoxicology. 2nd-3rd April
2013. Lisbon, Portugal.
M. Larguinho, J.L. Capelo, P.V. Baptista (2012) Colorimetric DNA adduct identification using
aptamer-gold nanoprobes. GOLD 2012 - The 6th International Conference on gold science,
technology and its applications. 5th-8th September 2012. Tokyo, Japan.
M. Larguinho, J.L. Capelo, P.V. Baptista (2012) DNA adduct identification using gold
nanoparticles for surface-assisted laser desorption/ionisation time-of-flight mass spectrometry.
XXXVII Jornadas Portuguesas de Genética. 28th-30th May 2012. Lisbon, Portugal.
M. Larguinho, C. Lodeiro, J.L. Capelo, P. Baptista (2011) Nucleotide identification using
nanoparticles for surface-assisted laser desorption/ionisation. International workshop on
nanoplasmonics for energy and the environment. 8th-10th June 2011. Sanxexo, Spain.
G. Doria, J. Conde, M. Larguinho, P. Baptista (2010) Multiplex genetic characterization via
noble metal nanoprobes. Trends in NanoTechnology 2010. 6th-10th September 2010. Braga,
Portugal.
M. Larguinho, G. Doria, J.L. Capelo, P.V. Baptista (2010). Speeding up DNA purification for
gold nanoprobe-based detection assays. Trends in Nanotechnology 2010. 6th-10th September
2010. Braga, Portugal.
M. Larguinho, H.M. Santos, G. Doria, J.L. Capelo, P.V. Baptista (2009). Ultrasonic
fragmentation of DNA and its importance in sample treatment for detection of specific
nucleotide sequences. Microbiotec09. 28th-30th November 2009. Vilamoura, Portugal.
Maria João Barreira, Cristina M. M. Almeida, Miguel Larguinho, Paula Alvito (2008). Patulin
in baby foods: optimization and validation of the SPE-HPLC-UV method. The World
Mycotoxin Forum. 17th-18th November 2008. Noordwijk, The Netherlands.
M. Larguinho, B. Pedras, T. Avilés, M.M.M. Raposo, B. Covelo, C. Lodeiro (2006). Rigid and
Flexible Schiff-base Bithiophene-Crown Ether ligands for metal ion detection. Synthesis and
characterization. XX National Encounter, Portuguese Chemistry Society. 14th-16th December
2006. Caparica, Portugal.
B. Pedras, M. Larguinho, C. Lodeiro, A.J. Parola, T. Avilés, M. Raposo, R.F. Batista, S.P. Costa
(2006). Synthesis of Bithiophene Derivatives as Emissive Devices. 4th National Encounter of
REQUIMTE. 31st March - 1st April 2006. Fátima, Portugal.
Participation in research projects
Research assistant: Project PTDC/BIO/66514/2006: "Sensitive and selective detection of
DNA/RNA based on functionalised gold nanoparticles – application to the detection of
pathogen agents; detection of mutations and RNA quantification”. Supervision by Prof. Pedro
Viana Baptista.
Research assistant: Project PTDC/SAU-BEB/66511/2006: “Nanodiagnostics: gold nanoprobes
for molecular identification - application to the detection of pathogen agents; detection of
mutations and RNA quantification”. Supervision by Prof. Pedro Viana Baptista.
Other skills/activities
- Scientific training in the laboratory of nutrition, toxicology and contaminants (INSA).
Development of analytical methods (HPLC and LC-MS) for identification and quantification of
the mycotoxin patulin in apple products. Supervision: Dr. Paula Alvito, MSc Maria Barreira.
- Scientific training in the plant genetics laboratory (ISA - UTL). Learning of cell biology
techniques and fluorescence microscopy towards the study of cell epigenetic machinery and
chromatin organisation patterns in several Arabidopsis tissues. Supervision: Prof. Nuno Neves,
Prof. Wanda Viegas and MSc Márcia Rosa.
- Scientific training in the photochemistry laboratory (FCT - UNL). Chemical synthesis and
characterisation of organic compounds for development of chemosensors. Supervision: Prof.
Carlos Lodeiro Espiño.
- Workshop “Advanced course in microalgae culturing and maintenance”. 9th-13th September
2013. BioPlant, University of Aveiro, Portugal.
- Workshop “Introduction to forensic sciences”. 29th September 2013. Coimbra, Portugal.
- Level I trainer/coach in shaolin Kung Fu and Tai Chi Chuan (YMAA Portugal).
Language
Native language: Portuguese
Other languages (according to Common European Framework of Reference):
Language
Reading
Writing
Conversation
English
Proficient
Proficient
Proficient
Spanish
Basic
Basic
Independent
French
Basic
Basic
Basic
Japanese
Basic
Basic
Basic