CV Celso REIS 2013

CURRICULUM VITAE
Name: Celso Albuquerque Reis.
Nationality: Portuguese
Address: Instituto de Patologia e Imunologia Molecular da Universidade do Porto – IPATIMUP,
Rua Dr Roberto Frias s/n, 4200 – Porto; Portugal
Telephone: 225570753; Mobile phone: 916946134
E-mail: [email protected]
Web link: www.ipatimup.pt
Academic Degrees:
Master in Sciences (Biology)
Faculty of Sciences and Technology of the University of Coimbra - Portugal.
Date: 16th October 1991.
Post-graduation: Oncobiology
University: Faculty of Medicine of the University of Porto, Portugal.
Date: 9th July 1993.
Post-graduation: PhD in Human Biology
University: Faculty of Medicine of the University of Porto, Portugal.
Date: 12th October 1998.
Professional Activities:
Present positions: Senior Researcher at IPATIMUP - Institute of Molecular Pathology and
Immunology of the University of Porto, Portugal.
Affiliated Professor of the Faculty of Medicine of the University of Porto.
Professor at Dept. Molecular Biology, ICBAS, University of Porto.
Past position: Post-Doc at the Glycobiology Laboratory at the Faculty of Health Sciences of the
University of Copenhagen, Denmark from 1999 to 2001.
Main scientific area of research:
Glycosyltransferases; Cancer.
Glycobiology; Protein Glycosylation; Oncobiology;
Academic Activities in the following Post-Graduate courses at the University of Porto:
• Post-graduate course in Medicine and Molecular Oncology (Faculty of Medicine
University of Porto).
• Master course in Molecular Medicine (Modules of Biopathology and Gastric Cancer).
• Post-graduate course of the University of Porto GABBA (Module of Oncobiology).
rd
• Seminar at 3 year of the Medical course at the Faculty of Medicine University of Porto.
• Free course on Oncobiology (Faculty of Medicine University of Porto).
• Workshop in Cancer Research IPATIMUP-ICBAS, University of Porto.
Published papers:
Publications: 91 articles
H-index: 25
Reviews: 5
Book chapters: 4
Patents: 3
Total citations ISI: 2303
1
1. Reis CA, David L, Nielsen PA, Clausen H, Mirgorodskaya K, Roepstorff P, Sobrinho-Simões
M (1997). Immunohistochemical study of MUC5AC expression in human gastric carcinomas
using a novel monoclonal antibody. Int J Cancer, 74, 112-121.
2. Nielsen PA, Mandel U, Therkildsen MH, Ravn V, David L, Reis CA, Wandall HH, Dabelsteen
E, Clausen H (1997). Loss of a mucin-like epithelial glycoprotein in oral and cervical
squamous cell carcinomas. Cancer Res, 57, 634-640.
3. Reis CA, Sørensen T, Mandel U, David L, Mirgorodskaya E, Roepstorff P, Kihlberg J, StigHansen JE, Clausen H (1998) Development and characterization of an antibody directed to
an αN-acetyl-D-galactosamine glycosylated MUC2 peptide. Glycoconj J, 15, 51-62.
4. Reis CA, Hassan H, Bennett EP, Clausen H (1998). Characterization of a panel of
monoclonal antibodies using GalNAc glycosylated peptides and recombinant MUC1.
Tumour Biol, 19, 127-133.
5. Price MR, Rye PD, Petrakou E, Reis CA, et al. (1998). Summary report on the ISOBM TD-4
Workshop: analysis of 56 monoclonal antibodies against the MUC1 mucin. Tumour
Biology, 19, 1-20
6. Reis CA, David L, Seixas M, Burchell J, Sobrinho-Simões M (1998). Expression of fully and
under glycosylated forms of MUC1 mucin in gastric carcinoma. Int J Cancer, 79, 402-410.
7. Nogueira AMMF, Machado JC, Carneiro F, Reis CA, Goett P, Sobrinho-Simões M (1998).
Patterns of expression of trefoil peptides and mucins in gastric polyps with and without
malignant transformation. J Pathol, 187, 541-548.
8. Reis CA, David L, Correa P, Carneiro F, DeBolós C, Garcia E, Mandel U, Clausen H,
Sobrinho-Simões M (1999). Intestinal metaplasia of the human stomach displays distinct
patterns of mucin (MUC1, MUC2, MUC5AC, MUC6) expression. Cancer Res, 59, 10031007.
9. Carvalho F, David L, Aubert J-P, López-Ferrer A, DeBolós C, Reis CA, Gärtner F, Peixoto
A. Alves P, Sobrinho-Simões M (1999). Mucin and mucin-associated carbohydrate antigens
expression in gastric carcinoma cell lines. Virchows Arch, 435:479-85.
10. Schmitt FC, Pereira MB, Reis CA (1999). MUC 5 expression in breast carcinomas. Hum
Pathol, 10, 1270-1.
11. Reis CA, David L, Carvalho F, Mandel U, DeBolós C, Mirgorodskaya E, Clausen H,
Sobrinho-Simões M (2000). Immunohistochemical study of the expression of MUC6 mucin
and co-expression of other secreted mucins (MUC5AC and MUC2) in human gastric
carcinoma. J Histochem Cytochem, 48:377-388.
12. Machado JC, Nogueira AMMF, Carneiro F, Reis CA, Sobrinho-Simões (2000). Gastric
carcinoma exhibits distinct types of cell differentiation: an immunohistochemical study of
Trefoil peptides (TFF1 and TFF2) and mucins (MUC1, MUC2, MUC5AC and MUC6). J
Pathol, 190:437-443.
13. Machado JC, Reis CA, Carneiro F, Sobrinho-Simoes M (2000). Primary signet-ring cell
carcinomas of the lung. Hum Pathol, 3:272.
14. Von Mensdorff-Pouilly S, Petrakou E, Kenemans P, van Uffelen K, Verstraeten AA,
Snijdewint FG, van Kamp GJ, Schol DJ, Reis CA, Price MR, Livingston PO, Hilgers J
(2000). Reactivity of natural and induced human antibodies to MUC1 mucin with MUC1
peptides and n-acetylgalactosamine (GalNAc) peptides. Int J Cancer, 86:702-12.
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15. David L, Silva F, Seruca R, Pinto M, Reis CA, Sobrinho-Simões M (2000). Correspondence
re: A. E. Biemer-Huttmann et al., Mucin core protein expression in colorectal cancers with
high levels of microsatellite instability indicates a novel pathway of morphogenesis. Clinical
Cancer Res, 6:4461-62.
16. López-Ferrer A, de Bolós C, Barranco C, M. Garrido, Isern J, Carlstedt I, Reis CA, Torrado
J, Real FX (2000). Role of fucosyltransferases in the association between apomucin and
Lewis antigen expression in normal and malignant gastric epithelium. Gut, 47:349-56.
17. Hassan H, Reis CA, Bennett EP, Mirgorodskaya E, Roepstorff P, Hollingsworth MA,
Burchell J, Taylor-Papadimitriou J, Clausen H (2000). The lectin domain of UDP-N-acetyl-Dgalactosamine:polypeptide N-acetylgalactosaminyltransferase-T4 directs its glyco-peptide
specificities. J Biol Chem, 275:38197-205.
18. Pereira MB, Dias AJ, Reis CA, Schmitt FC (2001). Immunohistochemical study of the
expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues. J
Clin Pathol, 54:210-3.
19. Carneiro F, Machado JC, David L, Reis CA, Nogueira AM, Sobrinho-Simoes M (2001).
Current thoughts on the histopathogenesis of gastric cancer. Eur J Cancer Prev, 10:101-2.
20. Li P, Arango ME, Perez RE, Reis CA, Bonfante EL, Weed D, Carraway KL (2001).
Expression and localization of immunoreactive-sialomucin complex (Muc4) in salivary
glands. Tissue Cell, 33:111-8.
21. Haselmann KF, Budnik BA, Olsen JV, Nielsen ML, Reis CA, Clausen H, Johnsen AH,
Zubarev RA (2001). Advantages of external accumulation for electron capture dissociation
in Fourier transform mass spectrometry. Anal Chem, 73:2998-3005.
22. Shaji KG, Holm B, Reis CA, Schwientek, Clausen H, Kihlberg J (2001). Chemoenzymatic
synthesis of derivatives of a T-cell-stimulating peptide which carry tumor-associated
carbohydrate antigens. J Chem Soc, 1:880-885.
23. George SK, Schwientek T, Holm B, Reis CA, Clausen H, Kihlberg J (2001)
Chemoenzymatic Synthesis of Sialylated Glycopeptides Derived from Mucins and T-Cell
Stimulating Peptides. J Am Chem Soc, 123:11117-11125.
24. Hanisch F-G, Reis CA, Clausen H, Paulsen H (2001). Evidence for glycosylationdependent activities of polypeptide N-acetylgalactosaminyl-transferases rGalNAc-T2 and T4
on mucin glycopeptides. Glycobiology, 11:731-740.
25. Pinto-de-Sousa J, David L, Reis CA, Gomes R, Silva L, Pimenta A (2002). Mucins MUC1,
MUC2, MUC5AC and MUC6 expression in the evaluation of differentiation and clinicobiological behaviour of gastric carcinoma. Virchows Arch, 440:304-10.
26. Silva E, Teixeira A, David L, Carneiro F, Reis CA, Sobrinho-Simoes J, Serpa J, Veerman E,
Bolscher J, Sobrinho-Simoes M (2002). Mucins as key molecules for the classification of
intestinal metaplasia of the stomach. Virchows Arch, 440:311-7.
27. Teixeira A, David L, Reis CA, Costa J, Sobrinho-Simões M (2002). Intestinal metaplasia of
the human stomach is colonized by Helicobacter pylori in the absence of MUC2 mucin
expression. J Pathol, 197:37-43.
28. Schwientek TJ, Bennett EP, Flores C, Thacker J, Hollman M, Reis CA, Behrens J, Mandel
U, Keck B, Schafer MA, Hazelmann K, Zubarev R, Roepstorff P, Hollingsworth MA, Clausen
H (2002). Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:
Polypeptide N-acetylgalactosaminyltransferases in drosophila, C. elegans and mammals:
One subfamily comprised of l(2)35Aa is essential in drosophila. J Biol Chem, 277:2262338.
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29. Mesquita P, Peixoto AJ, Seruca R, Hanski C, Almeida R, Silva F, Reis CA, David L (2003).
Role of site-specific promoter hypomethylation in aberrant MUC2 mucin expression in
mucinous gastric carcinomas. Cancer Lett, 189:129-36.
30. Silva F, Carvalho F, Peixoto A, Teixeira A, Almeida R, Reis CA, Bravo LE, Realpe L,
Correa P, David L (2003). MUC1 polymorphism confers increased risk for intestinal
metaplasia in a Colombian population with chronic gastritis. Eur J Hum Genet, 11:380-4.
31. Serpa J, Almeida R, Oliveira C, Silva FS, Silva E, Reis C, Le Pendu J, Oliveira G, Ribeiro
LM, David L (2003). Lewis enzyme (alpha1-3/4 fucosyltransferase) polymorphisms do not
explain the Lewis phenotype in the gastric mucosa of a Portuguese population. J Hum
Genet, 48:183-9.
32. Marcos NT, Cruz A, Silva F, Almeida R, David L, Mandel U, Clausen H, Von MensdorffPouilly S, Reis CA (2003). Polypeptide GalNAc-transferases, ST6GalNAc-transferase I and
ST3Gal-transferase I expression in gastric carcinoma cell lines. J Histochem Cytochem,
51:761-71.
33. Freire T, Medeiros A, Reis CA, Real FX, Osinaga E (2003). Biochemical characterization of
soluble Tn glycoproteins from malignant effusions of patients with carcinomas. Oncol Rep.
10:1577-85.
34. Mesquita P, Jonckheere N, Almeida R, Ducourouble MP, Serpa J, Silva E, Pigny P, Silva
FS, Reis C, Silberg D, Van Seuningen I, David L (2003). Human MUC2 mucin gene is
transcriptionally regulated by Cdx homeodomain proteins in gastrointestinal carcinoma cell
lines. J Biol Chem. 278:51549-56.
35. Ragupathi G, Koide F, Sathyan N, Kagan E, Spassova M, Bornmann W, Gregor P, Reis
CA, Clausen H, Danishefsky SJ, Livingston PO. (2003). A preclinical study comparing
approaches for augmenting the immunogenicity of a heptavalent KLH-conjugate vaccine
against epithelial cancers. Cancer Immunol Immunother, 52:608-616.
36. Pinto-de-Sousa J, Reis CA, David L, Pimenta A, Cardoso-de-Oliveira M (2004). MUC5B
expression in gastric carcinoma: its relationship with clinico-pathological parameters and
with expression of mucins MUC1, MUC2, MUC5AC AND MUC6. Virchows Arch. 444:22430.
37. Nogueira AM, Marques T, Soares PC, David L, Reis CA, Serpa J, Queiroz DM, Rocha GA,
Rocha AC (2004). Lewis Antigen Expression in Gastric Mucosa of Children: Relationship
With Helicobacter pylori Infection. J Pediatr Gastroenterol Nutr. 38:85-91.
38. Reis CA, Teixeira A, David L (2004). Role of mucins in Helicobacter pylori adhesion to the
gastric mucosa. Helicobacter 2:181.
39. Serpa J, Mendes N, Reis CA, Santos Silva LF, Almeida R, LePendu J, David L. Two new
FUT2 missense polymorphisms, 739G>A and 839T>C, are partly responsible for nonsecretor status in a Caucasian population from Northern Portugal (2004). Biochem J. 383:
469-74.
40. Marcos NT, Pinho S, Grandela C, Cruz A, Samyn-Petit B, Harduin-Lepers A, Almeida R,
Silva F, Morais V, Costa J, Kihlberg J, Clausen H, Reis CA (2004). Role of the human
ST6GalNAc-I and ST6GalNAc-II in the synthesis of the cancer-associated Sialyl-Tn antigen.
Cancer Res. 64:7050-7.
41. Santos-Silva F, Fonseca A, Caffrey T, Carvalho F, Mesquita P, Reis C, Almeida R, David L,
Hollingsworth M (2005). Thomsen-Friedenreich antigen expression in gastric carcinomas is
associated with MUC1 mucin VNTR polymorphism. Glycobiology. 15: 511-517.
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42. Kagan E, Ragupathi G, Yi SS, Reis CA, Gildersleeve J, Kahne D, Clausen H, Danishefsky
SJ, Livingston PO (2005). Comparison of antigen constructs and carrier molecules for
augmenting the immunogenicity of the monosaccharide epithelial cancer antigen Tn.
Cancer Immunol Immunother. 54(5):424-30.
43. Slovin SF, Ragupathi G, Fernandez C, Jefferson MP, Diani M, Wilton AS, Powell S,
Spassova M, Reis C, Clausen H, Danishefsky S, Livingston P, Scher HI (2005). A bivalent
conjugate vaccine in the treatment of biochemically relapsed prostate cancer: a study of
glycosylated MUC-2-KLH and Globo H-KLH conjugate vaccines given with the new semisynthetic saponin immunological adjuvant GPI-0100 OR QS-21. Vaccine. 23:3114-22.
44. Almeida R, Almeida J, Shoshkes M, Mendes N, Mesquita P, Silva S, Van Seuningen I, Reis
CA, Santos-Silva F, David L (2005). OCT-1 is over-expressed in intestinal metaplasia and
intestinal gastric carcinomas and binds to, but does not transactivate, CDX2 in gastric cells.
J Pathol; 207:396-401.
45. Marques T, David L, Reis C, Nogueira A (2005). Topographic expression of MUC5AC and
MUC6 in the gastric mucosa infected by Helicobacter pylori and in associated diseases.
Pathol Res Pract; 201(10):665-72.
46. Pudelko M, Lindgren A, Tengel T, Reis CA, Elofsson M, Kihlberg J (2006). Formation of
lactones from sialylated MUC1 glycopeptides. Org Biomol Chem. 4:713-20.
47. Serpa J, Mesquita P, Mendes N, Oliveira C, Almeida R, Santos-Silva F, Reis CA, Lependu
J, David L (2006). Expression of Le(a) in gastric cancer cell lines depends on FUT3
expression regulated by promoter methylation. Cancer Lett. 242(2):191-7.
48. Sorensen AL, Reis CA, Tarp MA, Mandel U, Ramachandran K, Sankaranarayanan V,
Schwientek T, Graham R, Taylor-Papadimitriou J, Hollingsworth MA, Burchell J, Clausen H
(2006). Chemoenzymatically synthesized multimeric Tn/STn MUC1 glycopeptides elicit
cancer specific anti-MUC1 antibody responses and override tolerance. Glycobiology,
16(2):96-107.
49. Durai Babu S, Jayanthi V, Niranjali D, Reis CA, Devaraj H (2006). Expression profile of
mucins (MUC2, MUC5AC and MUC6) in Helicobacter pylori infected pre-neoplastic and
neoplastic human gastric epithelium. Mol Cancer. 5(1):10 [Epub ahead of print].
50. Ferreira B, Marcos NT, David L, Nakayama J, Reis CA (2006). Terminal alpha1,4-linked Nacetylglucosamine in Helicobacter pylori-associated intestinal metaplasia of the human
stomach and gastric carcinoma cell lines. J Histochem Cytochem.;54(5):585-91.
51. Escrevente, C., Machado, E., Brito, C., Reis, CA, Stoeck, A., Runz, S., Marmé, A., Altevogt,
P., Costa, J. (2006) Different expression levels of alpha3/4 fucosyltransferases and Lewis
determinants in ovarian carcinoma tissues and cell lines. Int. J. Oncol. 3, 557-566.
52. Mesquita P, Raquel A, Nuno L, Reis CA, Silva LF, Serpa J, Van Seuningen I, Barros H,
David L (2006). Metaplasia--a transdifferentiation process that facilitates cancer
development: the model of gastric intestinal metaplasia. Crit Rev Oncog. 2006 Jul;12(12):3-26.
53. Brito C, Escrevente C, Reis CA, Lee VM, Trojanowski JQ, Costa J (2007). Increased levels
of fucosyltransferase IX and carbohydrate Lewis(x) adhesion determinant in human NT2N
neurons. J Neurosci Res. 2007 May 1;85(6):1260-70.
54. Pinho S, Marcos NT, Ferreira B, Carvalho AS, Oliveira MJ, Santos-Silva F, Harduin-Lepers
A, Reis CA (2007). Biological significance of cancer-associated Sialyl-Tn antigen:
modulation of malignant phenotype in gastric carcinoma cells. Cancer Lett. 249:157-70.
5
55. Pinho SS, Matos AJ, Lopes C, Marcos NT, Carvalheira J, Reis CA, Gärtner F (2007). Sialyl
Lewis x expression in canine malignant mammary tumours: correlation with
clinicopathological features and E-Cadherin expression. BMC Cancer; 7:124.
56. Costa NR, Mendes N, Marcos NT, Reis CA, Caffrey T, Hollingsworth MA, Santos-Silva
F.Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori
adhesion to gastric epithelial cells. World J Gastroenterol. 2008 Mar 7;14(9):1411-4
57. Marcos NT, Magalhães A, Ferreira B, Oliveira MJ, Carvalho AS, Mendes N, Gilmartin T,
Head SR, Figueiredo C, David L, Santos-Silva F, and Reis CA. Helicobacter pylori induces
b3GnT5 in human gastric cell lines, modulating expression of the SabA adhesin ligand sialyl
Lewis x. J Clinical Investigation. 2008, 118(6):2325-36.
58. de Oliveira JT, Pinho SS, de Matos AJ, Hespanhol V, Reis CA, Gärtner F. MUC1
expression in canine malignant mammary tumours and relationship to clinicopathological
features. Vet J. 2008 Oct 21. [Epub ahead of print]
59. Gomes J, Marcos NT, Berois N, Osinaga E, Magalhães A, Pinto-de-Sousa J, Almeida R,
Gärtner F, Reis CA (2008). Expression of UDP-N-acetyl-D-galactosamine: Polypeptide Nacetylgalactosaminyltransferase-6 in Gastric Mucosa, Intestinal Metaplasia, and Gastric
Carcinoma. J Histochem Cytochem. 57(1):79-86.
60. Barros R, Marcos N, Reis CA, De Luca A, David L, Almeida R. CDX2 expression is induced
by Helicobacter pylori in AGS cells. Scand J Gastroenterol 2009; 44:124-125.
61. Pinho SS, Osório H, Nita-Lazar M, Gomes J, Lopes C, Gärtner F, Reis CA. Role of Ecadherin N-glycosylation profile in a mammary tumor model. Biochem Biophys Res
Commun. 2009 Feb 20;379(4):1091-1096.
62. Barros R, Mendes N, Howe JR, Reis CA, de Bolos C, Carneiro F, David L, Almeida R.
Juvenile polyps have gastric differentiation with MUC5AC expression and downregulation of
CDX2 and SMAD4. Histochem Cell Biol. 2009 Jun;131(6):765-72.
63. Pinho SS, Reis CA, Paredes J, Magalhães AM, Ferreira AC, Figueiredo J, Xiaogang W,
Carneiro F, Gärtner F, Seruca R. The Role of N-acetylglucosaminyltransferase III and V in
the Post-Transcriptional Modifications of E-cadherin. Hum Mol Genet. 2009 Jul
15;18(14):2599-608. Epub 2009 Apr 29.
64. Pinho SS, Reis CA, Gärtner F, Alpaugh ML. Molecular plasticity of E-cadherin and Sialyl
Lewis x expression, in two comparative models of mammary tumorigenesis.
PLoS ONE 2009.
65. Magalhães A, Marcos NT, Carvalho AS, David L, Figueiredo C, Bastos J, David G, Reis
CA. Helicobacter pylori cag pathogenicity island-positive strains induce syndecan-4
expression in gastric epithelial cells. FEMS Immunol Med Microbiol. 2009 Aug;56(3):22332. Epub 2009 May 16.
66. Magalhães A, Gomes J, Ismail MN, Haslam SM, Mendes N, Osório H, David L, Lependu J,
Haas R, Dell A, Borén T, Reis CA. Fut2-null mice display an altered glycosylation profile
and impaired BabA-mediated Helicobacter pylori adhesion to gastric mucosa.
Glycobiology. 2009 19(12):1525-36
67. Silva LM, Carvalho AS, Guillon P, Seixas S, Azevedo M, Almeida R, Ruvoën-Clouet N,
Reis CA, Le Pendu J, Rocha J, David L. Infection-associated FUT2 (Fucosyltransferase 2)
genetic variation and impact on functionality assessed by in vivo studies. Glycoconj J.
2010 Jan;27(1):61-8.
68. Carvalho AS, Harduin-Lepers A, Magalhães A, Machado E, Mendes N, Costa LT,
Matthiesen R, Almeida R, Costa J, Reis CA. Differential expression of alpha-2,3sialyltransferases and alpha-1,3/4-fucosyltransferases regulates the levels of sialyl Lewis a
6
and sialyl Lewis x in gastrointestinal carcinoma cells. Int J Biochem Cell Biol. 2010
Jan;42(1):80-9.
69. Conze T, Carvalho AS, Landegren U, Almeida R, Reis CA, David L, Söderberg O. MUC2
mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia
and gastric carcinomas. Glycobiology. 2010 Feb;20(2):199-206.
70. Madeira PP, Reis CA, Rodrigues AE, Mikheeva LM, Zaslavsky BY. Solvent properties
governing solute partitioning in polymer/polymer aqueous two-phase systems: nonionic
compounds. J Phys Chem B. 2010 Jan 14;114(1):457-62.
71. Reis CA, Osorio H, Silva L, Gomes C, David L. Alterations in glycosylation as biomarkers
for cancer detection. J Clin Pathol. 2010 Apr;63(4):322-9.
72. Magalhães A, Reis CA Helicobacter pylori adhesion to gastric epithelial cells is mediated by
glycan receptors. Braz J Med Biol Res. 2010 Jul;43(7):611-8.
73. Magalhães A, Ismail MN, Reis CA. Sweet receptors mediate the adhesion of the gastric
pathogen Helicobacter pylori: glycoproteomic strategies. Expert Rev Proteomics. 2010
Jun;7(3):307-10.
74. Madeira PP, Reis CA, Rodrigues AE, Mikheeva LM, Chait A, Zaslavsky BY. Solvent
properties governing protein partitioning in polymer/polymer aqueous two-phase systems. J
Chromatogr A. 2011 Mar 11;1218(10):1379-84.
75. Pinho SS, Seruca R, Gärtner F, Yamaguchi Y, Gu J, Taniguchi N, Reis CA. Modulation of
E-cadherin function and dysfunction by N-glycosylation. Cell Mol Life Sci. 2011
Mar;68(6):1011-20.
76. Silva Z, Tong Z, Guadalupe Cabral M, Martins C, Castro R, Reis C, Trindade H,
Konstantopoulos K, Videira PA. Sialyl Lewis(x)-dependent binding of human monocytederived dendritic cells to selectins. Biochem Biophys Res Commun. 2011 Jun
10;409(3):459-64.
77. Marcos NT, Bennett EP, Gomes J, Magalhaes A, Gomes C, David L, Dar I, Jeanneau C,
Defrees S, Krustrup D, Vogel LK, Kure EH, Burchell J, Taylor-Papadimitriou J, Clausen H,
Mandel U, Reis CA. ST6GalNAc-I controls expression of sialyl-Tn antigen in
gastrointestinal tissues. Front Biosci (Elite Ed). 2011 Jun 1;3:1443-55.
78. Reis CA, Campos D, Osório H, Santos LL. Glycopeptide microarray for autoantibody
detection in cancer. Expert Rev Proteomics. 2011 Aug;8(4):435-7.
79. Pinto R, Carvalho AS, Conze T, Magalhães A, Picco G, Burchell JM, Taylor-Papadimitriou
J, Reis CA, Almeida R, Mandel U, Clausen H, Söderberg O, David L. Identification of new
cancer biomarkers based on aberrant mucin glycoforms by in situ Proximity Ligation. J Cell
Mol Med. 2011 Aug 30. doi: 10.1111.
80. Parreira P, Magalhães A, Gonçalves IC, Gomes J, Vidal R, Reis CA, Leckband DE, Martins
MC. Effect of surface chemistry on bacterial adhesion, viability, and morphology. J Biomed
Mater Res A. 2011 Dec 1;99(3):344-53.
81. Nolte S, de Castro Damasio D, Baréa AC, Gomes J, Magalhães A, Mello Zischler LF,
Stuelp-Campelo PM, Elífio-Esposito SL, Roque-Barreira MC, Reis CA, Moreno-Amaral AN.
BJcuL, a lectin purified from Bothrops jararacussu venom, induces apoptosis in human
gastric carcinoma cells accompanied by inhibition of cell adhesion and actin cytoskeleton
disassembly. Toxicon. 2012 Jan;59(1):81-5. Epub 2011 Nov 4.
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82. Gomes J, Magalhães A, Michel V, Amado I, Aranha P, Ovesen RG, Hansen HC, Gärtner F,
Reis CA, Touati E. Pteridium aquilinum and its ptaquiloside toxin induce DNA damage
response in gastric epithelial cells, a link with gastric carcinogenesis. Toxicol Sci. 2012
Mar;126(1):60-71
83. Madeira PP, Bessa A, Alvares-Ribeiro L, Aires-Barros MR, Reis CA, Rodrigues AE,
Zaslavsky BY. Salt effects on solvent features of coexisting phases in aqueous
polymer/polymer two-phase systems. J Chromatogr A. 2012 Mar 16;1229:38-47.
84. Pinho SS, Carvalho S, Cabral J, Reis CA, Gärtner F. Canine tumors: a spontaneous animal
model of human carcinogenesis. Transl Res. 2012 Mar;159(3):165-72. Epub 2011 Dec 21.
85. Pinho SS, Oliveira P, Cabral J, Carvalho S, Huntsman D, Gärtner F, Seruca R, Reis CA,
Oliveira C. Loss and recovery of Mgat3 and GnT-III mediated E-cadherin N-glycosylation is
a mechanism involved in Epithelial-Mesenchymal-Epithelial Transitions. PLoS ONE,
2012;7(3):e33191. Epub 2012 Mar 13
86. Marcos-Pinto R, Carneiro F, Dinis-Ribeiro M, Wen X, Lopes C, Figueiredo C, Machado JC,
Ferreira RM, Reis CA, Ferreira J, Pedroto I, Areias J. First-degree relatives of patients with
early-onset gastric carcinoma show even at young ages a high prevalence of advanced
OLGA/OLGIM stages and dysplasia. Aliment Pharmacol Ther. 2012 Jun;35(12):1451-9.
doi: 10.1111/j.1365-2036.2012.05111.x. Epub 2012 May 2
87. Tavares C, de Oliveira JT, Lopes C, Carvalheira J, Matos AJ, Rutteman G, Reis CA,
Gärtner F. Mucin 6 and Tn Antigen Expression in Canine Mammary Tumours: Correlation
with Pathological Features. J Comp Pathol. 2012 [Epub ahead of print].
88. Paredes J, Figueiredo J, Albergaria A, Oliveira P, Carvalho J, Ribeiro AS, Caldeira J, Costa
AM, Simões-Correia J, Oliveira MJ, Pinheiro H, Pinho SS, Mateus R, Reis CA, Leite M,
Fernandes MS, Schmitt F, Carneiro F, Figueiredo C, Oliveira C, Seruca R. Epithelial E- and
P-cadherins: Role and clinical significance in cancer. Biochim Biophys Acta. 2012 May
19.
89. Gomes J, Magalhães A, Carvalho AS, Hernandez GE, Papp SL, Head SR, Michel V, David
L, Gärtner F, Touati E, Reis CA. Glycophenotypic alterations induced by Pteridium
aquilinum in mice gastric mucosa: synergistic effect with Helicobacter pylori infection. PLoS
One. 2012;7(6):e38353. Epub 2012 Jun 13.
90. Pinho SS, Figueiredo J, Cabral J, Carvalho S, Dourado J, Magalhães A, Gärtner F,
Mendonça AM, Isaji T, Gu J, Carneiro F, Seruca R, Taniguchi N, Reis CA. E-cadherin and
adherens-junctions stability in gastric carcinoma: Functional implications of
glycosyltransferases
involving
N-glycan
branching
biosynthesis,
Nacetylglucosaminyltransferases III and V. Biochim Biophys Acta. 2012 Nov 5. doi:pii:
S0304-4165(12)00307-8. 10.1016/j.bbagen.2012.10.021. [Epub ahead of print]
91. Gomes C, Almeida A, Ferreira JA, Silva L, Santos-Sousa H, Pinto-de-Sousa J, Santos LL,
Amado F, Schwientek T, Levery S, Mandel U, Clausen H, David L, Reis CA, Osorio H.
Glycoproteomic analysis of serum from patients with gastric precancerous lesions. J
Proteome Res. 2013 Jan 14. [Epub ahead of print]
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Chapter in Books:
1. Reis CA, David L. Mucin expression in normal gastric mucosa, intestinal metaplasia
and gastric carcinomas. In: Digestive mucus, from research to clinical implications. Bara
J, Christen Y, Droy-Lefaix (eds.). IRVINN Editions, Paris, 2000, pg 125-129.
2. Reis C.A. Glycosylation. Encyclopedia of Cancer, 2nd Edition. Springer. 2008.
3. Reis C.A. Gastric Cancer. Encyclopedia of Cancer, 2nd Edition. Springer. 2008.
4. Reis C.A. Advances in chemoenzymatic synthesis of glycopeptides for cancer research
applications. In: Carbohydrate Chemistry: Chemical and Biological Approaches. A. Pilar
Rauter, T K Lindhorst. The Royal Society of Chemistry, Cambridge, 2010. Pgs: 142149.
Coordinator (PI) in the following projects:
“Production and Development of GalNAc-MUC1 and NeuAc Alpha2-6GalNAc-MUC1
Glycopeptides for immunotherapy in Cancer”, funded by Fundação para a Ciência e a
Tecnologia (POCTI/CBO/36376/2000; PI: Dr. Celso Reis).
“Molecular mechanisms of biosynthesis of altered mucin O-glycosylation in intestinal
metaplasia”, funded by Fundação para a Ciência e a Tecnologia (POCTI/CBO/44598/2002; PI:
Dr. Celso Reis).
"Identification of Glycosylation-associated genes induced by H. pylori in gastric cells: a glycomic
approach", funded by Fundação para a Ciência e a Tecnologia (POCI/SAU-OBS/56686/2004;
PI: Celso Reis.
“Molecular mechanisms of biosynthesis of sialylated cancer-associated mucin carbohydrate
antigens in gastric carcinoma”, funded by the Association for International Cancer Research
(AICR 05-088; PI: Celso Reis).
"Early detection of gastric cancer using serum biomarkers based on aberrant post-translational
modifications of O-glycoproteins", funded by Fundação para a Ciência e a Tecnologia
(PIC/IC/82716/2007; PI: Celso Reis)
“Glycomic Approach to unravel the role of Glycostructures in Gastric Carcinogenesis”, Institut
Mérieux, France; Merieux Research Program (Principal Investigator – C.A. Reis).
Supervisor of the following students:
Supervisor of the following Biologists: Andrea Patricia Cruz, Nuno Marcos, and Catarina
Grandela, grantees under the project POCTI/CBO/36376/2000 at IPATIMUP (From 2000-2003).
Supervisor of the Graduation Thesis of the Biologist Sandra Pinho, from the "Faculdade de
Ciências da Universidade do Porto" (2003).
Supervisor of the Graduation Thesis of the Biologist Bibiana Ferreira, from the "Universidade de
Aveiro". (2004-2005).
Supervisor of the lab-rotation of Cláudia Lopes, PhD student from the "Programa Gulbenkian de
Doutoramento em Biomedicina" (2004).
Co-supervisor of the advance training of the Medical student Anne Louise Sorensen in the
Glycobiology Lab. of the Faculty of Health Sciences of the University of Copenhagen –
Denmark, from 1999 to 2000.
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Supervisor of the Post-Doc Ana Carvalho at IPATIMUP, from 2006-2010.
Co-supervisor of the PhD Thesis of Salomé Pinho (The supervisor is Prof. Fátima Gärtner) at
IPATIMUP and Instituto de Ciências Biomédicas Abel Salazar. From 2006 to 2009.
Co-supervisor of the Master Thesis of Joana Gomes (The supervisor is Prof. Fátima Gärtner) at
IPATIMUP and Faculty of Medicine of Porto. From 2006 to 2007.
Supervisor of the PhD Thesis of Nuno Marcos at IPATIMUP and Faculty of Medicine of Porto.
From 2004 to 2008.
Supervisor of the PhD Thesis of Ana Magalhães at IPATIMUP and Faculty of Medicine of Porto.
From 2007 to 2011.
Supervisor of the PhD Thesis of Joana Gomes at IPATIMUP and Faculty of Medicine of Porto.
From 2007 to 2012.
Supervisor of the PhD Thesis of Catarina Gomes at IPATIMUP and Faculty of Medicine of
Porto. From 2009 to present.
Supervisor of the PhD Thesis of Diana Campos at IPATIMUP and Faculty of Medicine of Porto.
From 2011 to present.
Supervisor of the Post-Doc Pedro Madeira at IPATIMUP and FEUP, from 2010 to 2012.
Supervisor of the Post-Doc Inês Gonçalves at IPATIMUP (The supervisor is Doctor Cristina
Martins from INEB), from 2011 to present.
Supervisor of the Post-Doc Salomé Pinho at IPATIMUP, from 2010 to present.
Co-supervisor of the PhD Thesis of Sandra Carvalho (The supervisor is Doctor Salomé Pinho)
at IPATIMUP, from 2012 to present.
Member of the following organizations or consortia:
•
•
The Consortium for Functional Glycomics, USA.
The Euroglycoscience forum; European Science Foundation.
Reviewer of articles submitted to the following scientific journals:
• International Journal of Cancer, from 1999.
• Tumor Biology, from 2000.
• Laboratory Investigation, from 2002.
• Journal of Histochemistry and Cytochemistry, from 2005.
• BMC Cancer, from 2005.
• Clinical Cancer Research, from 2005
• Cancer Research, from 2006
• Gastroenterology, from 2007
• Glycobiology, from 2007
• Journal of Biological Chemistry, from 2007
• Carcinogenesis, from 2008
• Int J Biochem Cell Biol, from 2010
• FEMS Immunology and Medical Microbiology, from 2010.
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Evaluator as an Expert for scientific projects submitted to:
• Agência de Inovação Portuguesa, from 2002.
• Sixth framework programme of the European Union, from 2003.
• Spanish Ministry of Science, from 2006.
• Seventh framework programme of the European Union, 2007, 2008, 2010, 2011, 2012.
• Australian Scientific Funding agency, 2007.
• Institute Pasteur - Paris, 2007 and 2010.
• Italian scientific agency from the PIEMONTE region, from 2008.
• EYSED-ETAK, Greece, 2010.
• Unity through Knowledge Fund, Croatia, 2010.
Patents:
1) “Inhibitors of polypeptide GalNAc-transferase lectin domains and functions directed by these”.
USA-Patent.
2) “Compositions comprising organometallic molybdenum compounds for treating cancer”.
European Patent - PT2004000004.
3) “Chitosan Microspheres”. Application number US: 61642587.
Monoclonal antibodies developed that are commercialized by biotechnology companies:
1) Monoclonal antibody to the human mucin MUC5AC (MAB2011, Clone CLH2).
Commercialized by: CHEMICON INTERNATIONAL INC.
28835 Single oak drive, Temecula, CA 92590; California, USA.
2) Monoclonal antibody to the human mucin MUC5AC (NCL-MUC5). Commercialized by:
NOVOCASTRA LABORATORIES Ltd.
Balliol Business Park West, Benton Lane, Newcastle upon Tyne; NE12 8EW, United
Kingdom.
3) Monoclonal antibody to the human mucin MUC6 (NCL-MUC6). Commercialized by:
NOVOCASTRA LABORATORIES Ltd.
Balliol Business Park West, Benton Lane, Newcastle upon Tyne; NE12 8EW, United
Kingdom.
Languages: Fluent in English, Portuguese, Spanish.
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