MSc in Biochemistry

Departamento de Química
MSc in Biochemistry
Mestrado em Bioquímica
Information Package/Course Catalogue
Information on degree programmes
•
General description:
•
Qualification awarded:
•
Admission requirements:
•
Educational and
professional goals:
Biochemistry has been playing an important role in Biomedicine
research and biotechnological development as well as in medicine
advances, with special reference to the drug development and gene
therapy. This course will create an opportunity for the students to
become specialists in Clinical Biochemistry and to develop their skills
further in this discipline.
This course will provide knowledge in the fundamental biochemical
and genomics aspects of pathological conditions. The concepts and
techniques available for detection or identification of several diseases
will be emphasized, taking always into account, throughout the
course, the ethical aspects of genetic manipulation and the use of
laboratory animal in scientific research.
This course will be attractive not only to recently graduate candidates
but also to professionals interested in scientific and technological
research to whom the knowledge of the most recent advances in
Clinical Biochemistry may contribute to self valorization.
M. Sc
BSc
in
Biochemistry,
Biology,
Biotechnology,
Chemistry,
Environmental Sciences and candidates with a medicine background
This course intends to provide knowledge on biochemical concepts
beneath pathological conditions, the available methodology for its
detection and comprehension as well as the biochemistry contribution
for therapy development as well as the use of new biocompatible
materials, biological or non-biological, for health care.
The professional and educational goals of this course are:
- To provide post-graduation in Clinical Biochemistry;
- To create professionals with knowledge and skills to define
strategies and to execute projects in scientific research, quality control
and clinical trials;
- To provide knowledge on bioinformatics and molecular simulation
to investigate problems of Clinical Biochemistry;
- To provide skilled professionals to select and interpret information,
to elaborate reports and to communicate scientific results to an
audience of specialists;
- To encourage self-oriented scientific research and learning;
- To create post-graduates with initiative and competence for
employment market inclusion and self-employment;
The post-graduate will have habilitations to integrate laboratorial
groups of clinical trials, biomedicine research and forensic
investigation and in pharmaceutical and cosmetic industry.
•
Access to further studies:
Able to follow to PhD.
•
Final test, if any:
No final exam
•
Explained in each course module
•
Head of Department of Chemistry
Examination and
assessment regulations:
ECTS departmental coordinator:
•
Course structure diagram with credits (60 per year):
1ºAno
UECTS
Bioinformatics and Biochemical Simulation
4
Bioinformática e Simulação Bioquímica
Experimental Design
4
Planeamento Experimental
Ética da Investigação Científica e Tecnológica em Ciências da Vida
Quality Control
2
5
Controlo da Qualidade
Clinical Biochemistry
6
Bioquímica Clínica
Clinical Microbiology
5
Microbiologia Clínica
Advanced Biochemical Methods
5
Métodos Avançados em Bioquímica
Stress and Cellular Death
5
Stresse e Morte Celular
Lectures in Biochemistry and Biomedicine
10
Temas Avançados em Bioquímica Médica
Biochemical Pharmacology
6
Farmacologia Bioquímica
Pharmaceutical Chemistry
5
Química Farmacêutica
Biomaterials
3
Biomateriais
Total
60
2ºAno
Research Seminar
1
Seminário em Investigação
Advanced Courses
9
Cursos Avançados
Master Thesis / Project
50
Dissertação / Projecto
Total
60
Description of individual course units
Course title:
Bioinformatic and Biochemical Simulation
Bioinformática e Simulação Bioquímica
Course code:
Not applicable
Type of course:
Master
Level of course:
Speciality
Year of study:
1º
Semester/trimester:
semester
Number of credits allocated: 4 UECTS
Name of lecturer:
Objective of the course:
Prerequisites:
Course contents:
Recommended reading:
Teaching methods:
Assessment methods:
Language of instruction:
Rui Ferreira, João Paulo Ramalho, Luis Loura, António do Canto, Isabel Ferreira
To know and to understand and to valorise concepts of structural and functional
genomic; to information technology to organise, analyse and distribute biochemical
information; to techniques of molecular simulation (molecular dynamic and Monte
Carlo method), as well as its capacity to properties foresight. Give capacity to use
information technology to organise, analyse and distribute biochemical information
in spite of obtain response for complexes problems of life; to delineate and execute
in group search in informatics bases to identify nucleotides and aminoacids
sequences, protein domain and structure; to identify molecular pathologies and
contribute to develop new diagnostic methods and therapy; planning molecular
simulation with adequate selection of necessary parameters to its execution;
Execute the validation of used models in simulations, extract an calculate wanted
properties as well as estimate the errors which affect the results simulation
No applicable
1. Historical perspective of Bioinformatics. 2. Data bases and SRS. 3. Algorihtms for
sequences alignment 4. Sequences search. Motifs, profile and domains. 5. Servers
and tools for genome analysis. 6. Genes and regulator sequences identification. 7.
Analysis of biochips. 8. Phylogenetic analysis. 9. Genome and cancer, from genotype
to phenotype. 10. Three-dimensional structure of proteins. Mono and threedimensional alignments. 11. Structural data Bases. The Protein Data Bank. The
format of type files .pdb. 12. Protein modelling by homology. Modelling by homology
based on Web. 13. Introduction to Monte-Carlo simulations. 14. Introduction to
Molecular dynamic simulations of temporal behaviour of macromolecules.
Applications to a proteins and cell membranes models. 15. Results analysis of
simulation. Analysis of equilibrium simulation. Radial distribution functions.
Mechanical properties. Fluctuations. Correlation functions. Dynamic proprieties.
Baxevanis, A.D. & Ouellete, B.F.F. (2005) – A practical Guide to the Analysis of
Genes and Proteins, 3rd edition, Cold Spring Harbor, New York.
Mount, D.W. (2004) – Bioinformatics: Sequence and Genome Analysis, 2nd edition,
Cold Spring Harbor, New York.
Tamar, S. (2002) – Molecular Modeling and Simulation, Spring-Verlag, New York.
The classes run in informatics laboratories involving data bases using and software
for analysis nucleotides and aminoacids sequences in spite of search or create data
bases with biochemical information. Tutorial classes involve instants of individual
contacts or in little teams where search, read occur and assisted-interpretation of
practical works contribute to prepare with success the reports and its oral
presentation.
Continuous evaluation of practical work (30% class.),
reports (40% class.)
oral presentation of reports(30% class.)
Portuguese
Experimental Design
•
Course title:
•
Course code:
•
Type of course:
Compulsory
•
Level of course:
Advanced
•
Year of study:
1st Year
•
Semester/trimester:
1st Semester
•
Planeamento experimental
Number of credits
allocated (workload
4 ECTS
•
Paulo Infante
Name of lecturer:
Objective of the course
(expected learning
outcomes and
competences to be
acquired):
To study basic principles and concepts of experimental design and to
provide students with essential statistical tools in the analysis of several
experimental designs.
To develop student’s capacity to selected and organize information, in a
critical way.
To understand some fundamental statistical tools of experimental
designs.
To select the appropriated regression model.
To validate different parametric approaches based on their objectives.
To recognize and to know how and when to apply nonparametric
approaches.
To stimulate autonomous learning and adaptation to new situations.
To use correctly and rationally statistical software.
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An introductory course in Probability and Statistics.
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Prerequisites:
It consists of 2 sections:
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Course contents:
Analysis of Variance:
Analysis of variance models: fixed effects (single and multiple factor),
random effects (single and multiple factor) and mixed effects.
Multiple comparisons
Complete and incomplete block designs. Latin square designs.
Non parametric approaches.
Regression Analysis:
Simple linear regression model and multiple regression model
(estimation, inference, prediction, model adequacy and validation).
Correlation.
Nonlinear Regression.
•
Recommended reading:
Montgomery, D. C. (2004) – Design and Analysis of Experiments, 6th
Ed., John Wiley.
Montgomery, D. C.; Peck, A. E.; Vining, G. C. (2001) - Introduction to
Linear Regression Analysis, 3rd Ed., John Wiley.
Steel, R., Torrie, J., Dickey, D. (1996) - Principles and Procedures of
Statistics: A Biometrical Approach, 3rd Ed., McGraw-Hill Companies.
•
Teaching methods:
Theoretical-practical lessons mainly lectured with a blackboard, with e-
learning tools, and transparencies.
Motivation of student’s attendance to the classroom and student’s
continuous work.
Introduction to theoretical concepts appealing to different areas of
applications to illustrate the importance of course contents.
Exercises with emphasis in the resolution of real problems, to motivate
interest in the course and to demonstrate its utility.
To stimulate individual and group participation within the classroom and
at home.
To emphasize the critical analysis and interpretation of data, appealing
to software outputs as much as possible.
•
Assessment methods:
• Language of instruction:
To privilege continued evaluation carrying out one test plus one group
project.
If continuous evaluation is not feasible for the student, a final
examination is possible, but the group project is still required although
with lesser weight for final grade.
Portuguese or English if necessary.
Ética
Quality Control
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Course title:
•
Course code:
•
Type of course:
Compulsory
•
Level of course:
Specialized
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Year of study:
3rd
•
Semester/trimester:
Semester
•
Controlo da Qualidade
Number of credits
allocated:
5
•
Cristina Sousa
Name of lecturer:
•
Objective of the
course (expected
learning outcomes and
competences to be
acquired):
•
•
•
To understand the importance of Quality for organizations. To know management
and planning tools in process improvement. To apply a methodology for monitoring
a process with statistical process control. To know a form of sampling activity. To
know general requirements for the compentence of calibration and testing
laboratories.To know Quality management Systems, Environmental management
Systems and Occupational Health and Safety management Systems and
implementation. To know food safety management system: Hazard Analysis and
Critical Control Point (HACCP).
Prerequisites:
Course contents:
Recommended
reading:
General Concepts in Quality. Quality politics and aims. Costs of quality. Quality
Tools. Introduction. The seven management and planning tools. Other tools.
Sampling. Definition. Samples. Sampling. Sampling plans. Quality in sampling.
Measurements. General Requirements for the Compentence of Calibration and
Testing Laboratories (ISO/IEC 17025:2005). Statistical Quality Control. Introduction.
Control Charts. Quality Management.Quality Management Systems (ISO
9000:2000). Environmental Management Systems (ISO 14001:1996). Occupational
Health and Safety Management Systems (OSHAS 18001:1999/NP 4397). Integrated
system. HACCP System. Introduction (ISO22000:2005). The seven principles of
HACCP. Critical points determination. Application of HACCP system. HACCP vs. ISO
9000 Family.
Colecção “Fichas Técnicas”, 2ª Ed., Instituto Português da Qualidade.
Metrologia- em síntese, Instituto Português da Qualidade (2001).
J. Juran, A. Godfrey, Juran’s Quality Handbook, 5th Ed., McGraw-Hill (1999).
D. Montgomery Introduction to Statistical Quality Control, 4th Ed., John Wiley &
Sons (2000).
E. Princhard, Quality in the Analytical Chemistry Laboratory, John Wiley & Sons
(1997).
NP EN ISO/IEC 17025:2005 – Requisitos gerais de competência para laboratórios de
ensaio e calibração.
P. Crosby, Quality is Still free: Making Quality Certain in Uncertain Times, McGrawHill (1995).
Leavenworth, Statistical Quality Control, John Wiley & Sons, (1996).
A. Ramos Pires, Qualidade – Sistemas de Gestão da Qualidade, 2ª Ed., Sílabo
(2000).
NP EN ISO 9000:2000 – Sistemas de gestão da qualidade, fundamentos e
vocabulario.
NP EN ISO 9001:2000– Sistemas de gestão da qualidade, requisitos.
NP EN ISO 14001:1999 – Sistemas de gestão ambiental, especificações e linhas de
orientaçãpo para a sua utilização.
NP 4397:2001 – Sistemas de gestão da segurança e saúde do trabalho,
especificações.
ISO 22000:2005 – Food safety managemnent systems requirements for any
organization in food chain.
Mortimore, C. Wallace, HACCP: A Practical Approach, 2nd Ed., Aspen Pub.(1998).
Codex Alimentarius Comission – Food Hygiene – Basic Texts, FAO/WHO (1999).
•
•
Teaching methods:
Assessment methods:
• Language of
instruction:
The teaching method emphasizes the quality, in the supplier, manufacturer and
client optical. The formative process involves the presentation of original texts,
articles and international documents related with quality with permanent student
knowledge interaction.
Monograph with the contribution of 30 % for final classification.
Final Exam (or two tests), with the contribution of 70 % for the final classification.
Portuguese
Clinical Biochemistry
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Curricular unit name:
•
Curricular unit Code:
•
Curricular unit type:
Specific Nuclear
•
Curricular unit level:
2sc Cycle
•
Curricular year:
1st Year
•
Semester/quarter:
Semester
•
Bioquímica Clínica
Number of credits
(based on work hours):
6 ECTS
•
Maria Teresa Tinoco
The aim of this program course is to provide the students with Knowledge to
understand the contribution of Biochemistry for the study of mechanisms of
diseases. The value of biochemical tests in diagnosis and monitoring of treatment,
in screening for disease or in the prognosis. Students will know the characteristics
of a clinical biochemistry laboratory, the variety of specimens used in biochemical
analysis and the correct collection and handling of biological material. As well be
familiar with the analytical performance of the test procedures, the interpretation
of results, error factors and the reference values.
Teacher Names:
•
Curricular unit
objectives (expected
learning results and
abilities to acquire):
•
Pre-requirements:
Not applied
•
The evolution of clinical laboratory science. Basic concepts in Clinical Biochemistry.
Diagnostic value of biochemical tests. Quality management. Biological specimens.
Physiology and pathophysiology of several organs and systems. Kidney and renal
function. Water and electrolytes balance. Liver and liver function. Disorders of
renal and liver function. Plasmatic proteins. Nutritional markers. Serum enzymes
on acute myocardial infarction, hepatic, pancreatic and muscular disease.
Plasmatic lipoproteins. Disorders of lipoproteins and risk of cardiovascular disease.
Cardiac markers. Carbohydrate metabolism. Diabetes mellitus. Hypoglycemia.
Inborn errors of carbohydrate metabolism.
•
BURTIS, Carl A. e ASHWOOD, Edward R. (2001) Tietz, Fundamentals of Clinical
Chemistry, 5th edition, W.B.Saunders Company, USA.
DELVIN, Thomas M. (2002) Textbook of Biochemistry with Clinical Correlations,
5th edition, John Wiley & Sons, Inc., New York.
FUENTES e CASTIÑEIRAS (1998) Bioquímica Clínica y Patologia Molecular, 2ª ed. ,
Ed. Queraltó, Reverté, Barcelona.
•
The teaching-learning process will be based in the individual work of the student
supported by theoretical and practical classes and by bibliography research.
Theoretical classes will be supported by audiovisual techniques and recommended
bibliography. Practical lectures, mainly laboratorial classes, will be planning and
executed by experimental work and articulated with theoretical concepts.
Curricular unit
content:
Recommended
lectures:
•
Teaching Methods:
Evaluation methods:
• Language used
The evaluation process will be based on the individual work and on the progress in
the theoretical and practical components of this curricular unit. Practical
component will be continuously evaluated by the student assiduity, previous
preparation and planning of experimental work and laboratorial work team
performance. The individual final evaluation will be calculated by: a) Pratical
evaluation b) Presentation of a work selected between those done in the classes.
The theoretical component will be evaluated in two possible ways: two tests done
through and at the end of the semester or by a final exam.
Portuguese
Name
Code
Type
Level
Year of study
Semester/trimester
Number of credits
Name of lecturer(s)
Objective
of
the
course
Prerequisites
Course contents
Recommended
readings
Teaching methods
Assessment
methods
Language of
instruction
Clinical Microbiology
Microbiologia Clínica
Compulsory
Advanced
1st Year
1st Semester
5 ECTs
Artur Marinho; Saul Semião-Santos
Clinical Biochemistry constitutes a domain sustained by two main pillars: the clinical
analytic lab and all biochemical methods which one can use there. This particular
curricular unit of Clinical Microbiology pretends to train the clinical biochemist with
substantial knowledge concerning the study of microbial infection: ethiology,
pathology, sintomatology, prophylaxis, epidemiology and laboratory diagnosis. On the
other hand it will aim the study of antibiotics and chemotherapic activities towards the
infectious agents. Finally it will considerer the clinic analytical lab at hospital level.
1. Capability for understanding the English language. 2. Capability for interpretation of
experimental results. 3. Skills on manipulation of optical microscopy. 4. Some skills on
management of most popular lab instruments.. 5. Basic know-how of biochemistry and
biology.
Theoretical
1. The Microbiologist and the Clinical Analytical Lab security and quality control. 2.
Selection, collection, manipulation and transportation of clinical samples for
microbiological analysis. 3. Sterilization, pasteurization, disinfection and asepsis. 4.
The microbiological agents and chemotherapy of viral and bacterial infections.. 5.
Laboratory diagnosis of infectious diseases. 6. Clinical bacteriology: classification,
morphology and physiology. Normal microbiotic flora. Estaphylococcus, Streptococcus,
Corynebacteria, Clostridium, Neisseria, Enterobacteria, Vibrio, Campylibacter,
Pseudomonas, Bordetella, Mycoplasma, Ureaplasma, Mycobacterium, Actinomyces,
Nocardia, Chlamydia, Rickettsia, Brucella e Yersinia. 7. Clinical Micology: morphology
and general biology. Immunity and chemotherapy of fungal infections. Pathogenic
fungi: superficial, opportunistic, systemic and subcutaneous. Pneumocystes carinii. 8.
Clinical virology: viral structure and classification of vírus infecting humans. Diagnosis,
pathogeny, prevention and control of infectious virus. Respiratory vírus, smallpox,
rubéola, enterovirus, hepatitis, herpes, diarrhoeas, arbovirus, rabies, retrovirus and
neoplasic virus. 9. Clinical Parasitology: General biology of protozoans and helminths.
General characteristics of groups. Pathogeny and chemotherapy of parasitic diseases.
Malaria, toxoplasmosis, criptosporidiosis, amebíasis, giardíasis, leishmaniosis,
tripanossomíasis, enterobíasis, trichuriasis, ascariasis, toxocariasis, trichinosis,
lymphatic filaríasis, onchocercíasis, teníasis, hidatic disease, paragoníasis,
chlonorchiasis ans schistosmiasis. 10. Infections caused by microorganisms: skin and
wounds; bone and joints; eye, hear, nose and teeth. High, median and inferior
respiratory airways. Enteric and food poisoning; urinary tract; central nervous system;
bacteremias; phetus and newborn; sexual transmitted diseases; imunocompromised
patient; nosocomial and hospital infection control.
Pratical
1. Isolation and identification of some microorganisms earlier described. 2 –
Bacteriological examination of fezes, urine and other biological fluids. 3 –
Antibiograms. 4 – Several serological tests for antibody detection: IFAT, ELISA, DAT,
SDS-PAGE and WESTERN BLOT. 5 – PCR technique in the diagnosis of viral infections.
1.Murray PR, Baron EJ et al. (1999). MANUAL OF CLINICAL MICROBIOLOGY. 7th Ed.,
American Society of Microbiology. 2. Sherris JC, Champoux JJ e al. (1990). MEDICAL
MICROBIOLOGY. 2nd Ed. Elsevier Science Publ.. ISBN: 0444015086. 3. Isenberg H
(2004). CLINICAL MICROBIOLOGY PROCEDURES.2nd Ed. ISBN: 1555812430. 4.
Prescott LM e al. (2005). MICROBIOLOGY. 6th ed. WBC/McGraw-Hill, Int.
Structured presentation. Elaboration of practical studies, presentation and discussion.
Practical part (30%): Continued evaluation – preparation, presentation and discussion
of lab work reports; Theoretical part (70%): Elaboration of a scientific monography
with presentation followed by general debate.
Portuguese or English if necessary
Advanced Methods in Biochemistry
Name
Métodos Avançados em Bioquímica
Code
Type
Major
Level
Advanced
Year of study
1st Year
Semester/trimester
1st Semester
Number of credits
5,0
Name of lecturer(s)
António Neto Vaz, Célia M. Antunes
Objective
course
of
the
One important objective of this course is providing the student with a more profound
insight into the biomolecules quantitative and qualitative determination and
purification.
Prerequisites
Course contents
1 – Spectral methods of analysis of biomolecules for qualitative and quantitative
determinations
2 – Biosensors
3 – Immunochemistry methods
Recommended
readings
Skoog, D.A., Holler, F.A., Nieman, T.A.(1999), Principles of instrumental analysis, 5ª
ed., Londres , Sauders College Publisshing.
Spichiger-Keller, U.E.(1998),
Chemical sensors and biosensors for medical and
biological applications, Berlin, Wiley-VCH
Keith Wilson & John Walker (2000). Principles and techniques of practical Biochemistry
(5th ed.). Cambridge University Press, UK.
T. Chard (1990).Laboratory techniques in biochemistry and molecular biology: an
introduction to RIA and related techniques, 4th edition. Ed. R.H. Burdon and P.H.
Kemeny,D.M. (1991), A practical guide to ELISA. Pergamon Press, Oxford, UK.
Teaching methods
Assessment
methods
Classroom lectures; Classroom problem solving sessions; Lab sessions
Evaluation throughout semester as well as during examination period
During examination period: written closed-book exam complemented with oral
examination, written open-book exam; During semester: graded lab sessions
Language of
instruction
Portuguese or English
Course title:
Stress and Cell Death
Stresse e Morte Celular
Course code:
Not applicable
Type of course:
Master
Level of course:
Elective specialized
Year of study:
1º
Semester/trimester:
semester
Number of credits allocated: 5 UECTS
Name of lecturer:
Objective of the course:
Prerequisites:
Course contents:
Recommended reading:
Teaching methods:
Assessment methods:
Language of instruction:
Rui Manuel Alves Ferreira, Isabel Alves Pereira Ferreira
To know and to understand theoretical and experimental information in domain of
stress and cell death that includes terms, concepts, factors, principles and chemical
reactions related with cell processes where occur formation of radicals and nonradicals species inductors of stress, using models organisms to study enzymatic and
non-enzymatic antioxidants mechanisms and its relation with stress, nutrition, cell
death and several clinic pathologies. To apply the acquires knowledge to detect and
to characterise events of stress and cell death at physiological, enzymatic and DNA
level, to obtain results which interpretation and discussion allow to delineate in
group and using informatics bases, work laboratorial planes to improve the
knowledge about stress and cell death with biomedical or biotechnological
applications
No applicable
1.Oxygen is a toxic gas. 2.Cell processes implicated in ROS formation and RNS 3.
Models organisms used in stress studies. 4. Reactives species with biochemical
importance, transition metals, sulphur, hidroxyl, superoxide, peroxyl, alkoxyl and
nitric oxide. 5. Non-radicals reactive species, hydrogen peroxide, hypochlorous acid,
singlet oxygen and peroxynitrite. 6. Enzymatic and non-enzymatic antioxidants
mechanisms. 7. Oxidative stress, adaptation, damages, repair and death. Detection
of free radicals and other reactive species. 9.Biotransformation and stress. 10.
Reactive species, inflamatory states, aging, nutrition, pathologies and therapy.
Devlin, T. M. (2005) Textbook of Biochemistry with Clinical Correlations, 6th ed., John
Wiley & Sons, Inc, New York.
Collery, P. et al. (2006) – Metal Ions in Biology and Medicine, Vol. 9, John Libbey
Eurotext, Paris
Alessio, H.M. & Hagerman, A.E. (2006) – Oxidative Stress, Exercise and Aging,
Imperial College Press, London.
The lectures are plenary lessons structured in scientific method, which combine
subject exposition related with agents, conditions and mechanisms of stress
response with bibliographic-assisted search, generating capacities to understand
strategies and to apply methodologies which allow identify, detect, and characterise
new situations and response mechanisms to stress, with application in clinic
pathologies and therapeutic. The practical classes take place in teaching laboratories
where the students execute laboratory-assisted work following protocols previously
supplied by the teacher or delineated by the student, resorting to bibliographic
information that simulate, detect or answer to standard conditions of stress. Tutorial
classes involve instants of individual contacts or in little teams where search, read
occur and assisted-interpretation of scientific bibliographic information which
contribute to prepare oral presentation of actual scientific papers.
Theoretical component: oral presentation of two scientific papers (50% class.)
Practical component: experimental work and report (25% class.),
oral presentation and discussion of results (25% class.)
Portuguese
Lectures in Biochemistry and Biomedicine
•
Course title:
•
Course code:
•
Type of course:
Core, elective
•
Level of course:
2º Ciclo
•
Year of study:
1º
•
Semester/trimester:
1 Semester
•
Temas Avançados em Bioquímica Médica
Number of credits
allocated (workload
based):
10
•
Teresa Tinoco, Ofélia Bento, Célia Antunes, Rui Ferreira e Rosário Martins
Name of lecturer:
Objective of the
course (expected
learning outcomes and
competences to be
acquired):
The aim of this course is to provide knowledge in the domain of the medical
biochemistry. Specifically, it is intended that the students acquire
knowledge that will allow them to understand the biochemical mechanisms
underlying some pathological states. The students are expected to endow
the biochemical concepts and mechanisms of pathophysiology and to
develop the ability to establish correlation between its different aspects.
•
Prerequisites:
Not applicable
Course contents:
Module I: Lipidaemia e cholesterolaemia. Cholesterol: distribution and
function. Metabolism of cholesterol and biliary acids. dislipidaemia:
Classification and Related diseases; Prevention and therapy.
Module II: Pancreatic beta cell and Diabetes Mellitus: from physiology to
pathology. β-cell dysfunctions: causes and consequences.
Module III: Metabolism and metabolic pathologies. Metabolic pathways.
Metabolic correlations and metabolic control and regulation. Clinical
correlations. Tracers with interest Biochemical markers with interest on
diagnosis and monitoring of some metabolic pathologies.
Module IV: Genetic Pathologies.
Module V: Molecular therapy
•
•
•
Recommended
reading:
Scientific papers indicated in each module of this course.
•
Teaching methods:
Lectures about the state of the art of each module.
•
Assessment methods:
Oral presentations and discussion of papers related with each theme.
The final classification will be the averaged (ECTS) classification obtained in
each different module.
• Language of
instruction:
Portuguese
Biochemical Pharmacology
•
Course title:
•
Course code:
•
Type of course:
Major
•
Level of course:
Advanced
•
Year of study:
1st Year
•
Semester/trimester:
2nd semester
•
Farmacologia Bioquímica
Number of credits
allocated:
6,0
•
António Neto Vaz
Name of lecturer:
•
Objective of the
course (expected
learning outcomes and
competences to be
acquired):
•
To study the effects of chemical substances on living organisms.
To establish knowledge of the general principles of the actions of medicines.
Prerequisites:
Introduction
Scope of pharmacology
Role of pharmacology in the veterinary medical curriculum
Pharmacokinetics
•
Course contents:
Disposition and fate of drugs in the body
Passage of drugs across biologic membranes
Drug administration
Bioavailability
Mechanisms of drug elimination
Drug metabolism and excretion
Some aspects of drug dosage
Pharmacodynamics
Drug treatment and the importance of receptors
Type of receptors
Properties of “authentic receptors”
Analysis of binding and response
Responses in the patient
•
Recommended
reading:
Collective sessions concerned with theoretical content – 20 hours
•
Teaching methods:
Practical sessions applying knowledge within the context of the laboratory
– 30 hours
Support tutorial sessions concerning the elaboration of analysis protocols,
such as researching, gathering and treating data – 3 hours.
Practical laboratory assessment
•
Assessment methods:
• Language of
instruction:
Written assessment followed by oral assessment
Portuguese
Pharmaceutical Chemistry
•
Course title:
•
Course code:
•
Type of course :
Elective
•
Level of course:
Advanced
•
Year of study:
1º
•
Semester/trimester:
•
Química Farmacêutica
Semester
Number of credits
allocated (workload
based):
5 ECTS
•
António Paulo S. Teixeira
Name of lecturer:
•
Objective of the course
(expected learning
outcomes and
competences to be
acquired):
•
The main objective of this discipline is to give basic knowledge on
Pharmaceutical Chemistry. The student should know and understand
fundamental concepts and principles, namely the mechanisms of action of the
drugs, their classification, molecular mechanisms and structure-activity
relationships, pharmacokinetic parameters used in the study and evaluation of
drugs, and the discovery and development of new drugs. The student should
know and understand the main techniques and preparation methods, isolation
and analysis of some drugs.
Prerequisites:
1. Introduction to Pharmaceutical Chemistry
2. Drugs and their action: receptors as therapeutics targets
3. Quantitative structure-activity relationships (QSAR).
4. Chemical structure and pharmacokinetics parameters:
Distribution, Metabolism and Excretion of drugs; Prodrugs
•
Course contents:
Absorption,
5. Methods of search, discovery and isolation of new drugs; contributes from
Natural Product Chemistry, Asymmetric Synthesis, Heterocyclic Chemistry,
Combinatorial Chemistry and Supramolecular Chemistry
6. Development and production of new drugs.
7. Study of some important drugs: chemical structure, synthesis, mechanism of
action, structure-activity relationship, metabolism and applications
8. Isolation and analysis of drugs
- G. L. Patrick, “An Introduction to Medicinal Chemistry”, 3th ed., Oxford
University Press, Oxford, 2005.
- G. Thomas, “Fundamentals of Medicinal Chemistry”, John Wiley and Sons,
Chichester, 2003.
•
Recommended reading:
- E. J. Barreiro, C. A. M. Fraga,”Química Medicinal – As bases moleculares da
acção dos fármacos”, ArtMed Editora, 2001.
- C. Avendano, “Introducción a la Química Farmacéutica”, 2ª ed., McGaw HillInteramericana de Espana, 2001.
- R. B. Silverman, “The Organic Chemistry of Drug Design and Drug Action”, 2nd
ed., Academic Press, 2004.
The teaching/learning process is based in individual study supported by the
recommended bibliography and in notes obtained during classes or in research
made by the student.
Theoretical classes are exposition classes, during which could be resolved some
exercises or discussed any related topic.
•
Teaching methods:
At the laboratory component, practical works (laboratory and research) are
realized by the students organized in small groups (2/3 persons), concerning
practical examples of the theoretical topics related the separation, isolation,
identification and the synthesis of some drugs, doing information research,
planning and execution of laboratory work and doing the presentation and/or
discussion about theoretical topics.
The student is also motivated to solve, individually or in group, several
exercises, small works or to read and discuss several scientific papers.
The evaluation is based in the acquisition and comprehension of the knowledge
and the evaluation of the development of competences by the student in the
different components of the discipline: theoretical and laboratorial components.
•
Assessment methods:
• Language of instruction:
The evaluation of theoretical component is done by a final examination (or two
examinations tests). The laboratory component is estimated by continuous
assessment of the performance of the student in the classes, based on the
resolution (individual or in groups) of problems, elaboration and execution of
laboratory practice works and their reports, and based in the answers of oral
and/or written questions about laboratory work.
Portuguese
Biomaterials
•
Course title:
•
Course code:
•
Type of course
•
Level of course:
Advanced
•
Year of study:
1st
•
Semester:
2ndSemester
•
Number of credits:
•
Name of lecturer:
Biomateriais
Prof. P.J.M. Carrott
Objective of the
course:
The objective of this discipline is to present a panorama of the applications of
biomaterials in medicine and dentistry by considering the principal types of
biomaterial, their properties and their interaction with the organism.
•
Not applicable
•
Prerequisites:
Historical summary of biomaterials.
Introduction to materials science - types of material (metals, ceramics,
polymers, composites) and their properties; principles and methods of
mechanical, structural and surface characterisation.
•
Course contents:
Fundamentals of biomaterials - types of material (Ti, alloys, amalgams, calcium
phosphates, carbon, polyHEMA, UHMWPE, PMMA, PEG/PEO, PLA/PGA, PTFE,
bisGMA, ionomers, silicones, chitosan, collagen, others); hidrogels; scaffolds for
tissue engineering; surface films.
Biomaterial-tissue interactions - biofilm; protein adsorption; biocompatibility;
toxicology; bone integration; degradation.
Applications - substitution of joints, ossicles, tendons and ligaments;
cardiovascular
implants;
contact
lenses
and
ophthalmic
implants;
orthodontology; tissue engineering; slow release systems; biosensors.
Ethics and future perspectives.
Biomaterials. 2nd Edition. S.V. Bhat, Alpha Science International, 2005.
•
Recommended
reading:
•
Teaching methods:
Biomaterials Science: An Introduction to Materials in Medicine. 2nd Edition. B.
Ratner, A.S. Hoffman, F.J. Schoen and J.E. Lemons, Elsevier, 2004.
The Chemistry of Medical and Dental Materials. J.W. Nicholson, Royal Society of
Chemistry, 2002.
Theoretical classes including demonstrations and problems supported by
individual and group student research and study.
•
Assessment
methods:
Spot tests (T), exam (E), monography (M). Final mark =0.2T+0.5E+0.3M
• Language:
Portuguese. English in tutorial regime
•
Course title:
•
•
•
Course code:
Research Seminar
Seminário em Investigação
Type of course
Elective
Level of course:
Advanced
•
Year of study:
2nd Year
•
Semester:
Semester, modular
•
Number of credits:
1 ECTS
•
Name of lecturer:
Célia Antunes (all tutors)
The main objective of this course is to provide guidance for the students to
organize, present and discuss their individual work (scientific research or
project).
Discussion sessions (data clubs) will be organized in order to evaluate the
“work in progress” and any methodological problems.
•
Objective of the
course:
This course will provide the development of several skills:
Managing knowledge: Data sourcing, selection and interpretation; Data
evaluation and problem solving.
Technical Competence: Experimental design; Accuracy; Analysing data; IT
skills.
Self management: Planning; Time management.
Inter-relationship: team work, Decision taking; Learning from others;
Sharing; Receiving critique.
Presentation Skills: Written reports; Oral; Scientific paper; Quantitative
data presentation.
•
Prerequisites:
Not applicable
•
Course contents:
Results obtained on or data from literature related to the individual
research or project that each student has undertaken.
•
Recommended
reading:
Based on scientific papers related to the thesis or project.
•
Teaching methods:
Tutorials; Project supervision meetings; Paper summary examination;
Oral presentation (Literature only); Group discussion of results of the
individual project or research (tutorial regime).
•
Assessment methods:
Oral and poster presentations of experimental data (self or literature)
related to the undertaken research project (20% each). Written report
(30%). Paper summary examination (30).
• Language:
Portuguese, english or spanish
Advanced Courses
•
Course title:
•
Course code:
•
Type of course:
Elective
•
Level of course:
Advanced
•
Year of study:
2st
•
Semester/trimester:
Semestral, modular
•
Cursos Avançados
Number of credits
allocated:
9 ECTS
•
Célia Antunes & other
Name of lecturer:
•
Objective of the
course (expected
learning outcomes and
competences to be
acquired):
The main objective of this course is to develop the student’s interest for
scientific research and the competence to interpret data presented in
scientific papers and to assess them critically in order to draw appropriate
conclusions. To accomplish this task several modules on state of the art
topics will be proposed in different areas of Biochemical sciences.
The students are expected to develop the competence: to read critically a
scientific paper, to identify the key features of the study; to write a
scientifically suitable abstract for a research paper; to interpret data
presented in scientific papers and to assess them critically in order to draw
appropriate conclusions; Gain an understanding of a broad range of subject
areas in the biochemical sciences.
This course will provide the development of several skills:
Managing knowledge: Data sourcing, selection and interpretation; Data
evaluation and problem solving.
Technical Competence: Laboratory techniques; Experimental design;
Accuracy; Analysing data; IT skills.
Self management: Planning; Time management.
Managing interrelationship: Team work, Decision taking; Learning from
others; Sharing; Receiving critique.
Presentation Skills: Written reports; Oral; Scientific paper; Quantitative
data presentation.
•
Prerequisites:
Not applicable
•
Course contents:
This course is composed by 3 modules (3 ECTS each) on different subjects
of advanced biochemistry. The students will be able to choose between
several modules proposed each year.
•
Recommended
reading:
Scientific papers indicated in each module of this course.
•
Teaching methods:
Lectures: 36h
Practical: 45h
Tutorials: 6
Assessment methods:
Assessment methods may vary between modules. However oral
presentations, paper examination, and written reports are likely used.
The final classification will be the averaged (ECTS) classification obtained in
each different module.
•
• Language of
instruction:
Português