Cu(isaepy)2 - Biochemical Journal

www.biochemj.org
Biochem. J. (2011) 437, 443–453 (Printed in Great Britain)
doi:10.1042/BJ20110510
SUPPLEMENTARY ONLINE DATA
Giuseppe FILOMENI*1 , Simone CARDACI*1 , Ana Maria DA COSTA FERREIRA†, Giuseppe ROTILIO*‡ and
Maria Rosa CIRIOLO*‡2
*Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy, †Departamento de Quı́mica Fundamental, Instituto de Quı́mica, Universidade
de São Paulo, Av. Prof. Lineu Prestes 748, CEP 05508-900, São Paulo, SP, Brazil, and ‡Research Centre IRCCS San Raffaele Pisana, Via dei Bonacolsi, 00163 Rome, Italy
Figure S1 Dose–dependent effects of [Cu(isaepy)2] on phospho-AMPK,
phospho-p38MAPK and p53 in SH-SY5Y cells
SH-SY5Y cells were treated with different concentrations of [Cu(isaepy)2 ] (25, 50, 75 and 150
μM) or, as a control, with equal volumes of DMSO. At the indicated times, 30 μg of total protein
extract was loaded on to each lane for the detection of basal and phospho-activated levels of
AMPK and p38MAPK , as well as p53. GAPDH was used as a loading control. Western blots are
from one experiment and are representative of three that gave similar results. Molecular-mass
markers are shown on the left.
Received 21 March 2011/4 May 2011; accepted 6 May 2011
Published as BJ Immediate Publication 6 May 2011, doi:10.1042/BJ20110510
1
2
These authors contributed equally to the study.
To whom correspondence should be addressed (email [email protected]).
c The Authors Journal compilation c 2011 Biochemical Society
Biochemical Journal
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