Proteomics as a new approach to understanding Cystic Fibrosis

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Proteomics as a new approach to understanding Cystic Fibrosis
Proteomics Laboratory
at DG-INSA-Lisboa
Deborah Penque,PhD
Portuguese Proteomics Network
(www.procura-portugal.org)
Laboratory members
1 FQ, Ctro de Genét Hum; Lab Proteómica,
INSA-Lis (D Penque)
2 Hemoglob, Ctro de Genét Hum, INSA-Lis (L
Romão),
3 UBioinformatics, IGC (P Fernandes).
4 Lab Química, Univ Aveiro (F Amado & P
Domingos).
5 Lab MS, ITQB (AV Coelho).
6 Lab Biloqia das Plantas, ITQB, (CP Ricardo),
1.
2.
3.
4.
5.
6.
7.
8.
Requinte, UNL, (L Capelo)
U Amilóide, IBMC, Porto, (MJ Saraiva)
CMDT, IHMT (H Silveira)
IHMT (C Cunha).
INETI, Dep Biotec/UTPAM(A Domingos).
Dep. Quím/Bioquím, FCUL, U Lisboa (M
Florencio)
Serv Gastro/Hepatol, HCL (J Peneda).
Un Bioinformatic, FCUL (F Couto)
Coordenator:
Deborah Penque (deborah.penque@insa.min-saude.pt)
Delegates:
Ana V Coelho (varela@itqb.unl.pt)
Francisco Amado (famado@dq.ua.pt)
Pedro Fernandes (pfern@igc.gulbenkian.pt)
4th Meeting of the European
Proteomics Association
2010: A Proteomic Odyssey
Towards Next
Decades
23-27 October 2010, Estoril, Portugal
Guincho beach, Portugal
Proteomics Laboratory
at DG-INSA-Lisboa
•
Research
• Chronic Lung Diseases (Cystic Fibrosis, Asthma, COPD)
Chronic Lung Diseases (Cystic Fibrosis, Asthma, COPD)
(POCTI/MGI/40878/2001/POCI/ESP/44720/2002/ POCTI/SAUMMO/56163/2004)
•
•
Liver diseases (APDF; POCTI/SAU-IMI/55112/2004 )
Collaboration in other INSA-projects
Services (under implementation)
•
•
•
2DE/2DE-MAP analysis by software
Protein identification by MALDI/TOF/TOF
Disease diagnostic/prognostic-based on proteomics
(QCAIII, Saude XXI/2005)
Comparative Proteomics of
Chronic Lung Diseases
Cystic Fibrosis
Asthma
COPD
Biomarker Discovery
Cystic Fibrosis is a Chronic Lung Disease
 common lethal recessive
disorders in worldwide
with no effective therapy
 CFTR gene codes for a
Cl- channel protein of
apical epithelial cells
membrane
 chronic lung infection and
inflammation cause 95%
of CF mortality
Asthma and COPD share same
features with Cystic Fibrosis
 COPD is characterized by a
chronic airway limitation to
airflow, due to bronchitis or
emphysema.
 Asthma is characterized by
chronic airway inflammation
and remodelling.
Possible influences of CFTR gene mutations in
COPD and asthma phenotype have been reported
Proteomics Strategies (PROJECTS) for
Biormakers Discovery of Chronic Lung Diseases
nasal or bronchial brushing cells,
healthy subjects and
nasal or bronchoalveolar lavage
fluid (BALF)
Airway
System
sputum, lung biopsy / autopsy
patients with other chronic
lung diseases such as
asthma, COPD, etc
Controls
Blood
System
serum, plasma,
platelets, red blood
cells, lymphocytes
CF patients
Transgenic CF
mice
2D/PAGE &
DIGE MS
Cell Lines
PROTEOMICS
Technicalplatforms
LC/MS/M
S
Bioinformatics
human CF and non-CF
airway cell lines
cells expressing wt or
mutated CFTR
SELDI-TOF
Specific Biomarkers for CF Lung Disease
Systems
Biology

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