influence of seed maturity on early seedling vigor in wheat

influence of seed maturity on early seedling vigor in wheat

165
Kragujevac J. Sci. 28 (2006) 165-171.
INFLUENCE OF SEED MATURITY
ON EARLY SEEDLING VIGOR IN WHEAT
Veselinka Zečević1, Desimir Knežević2, Danica Mićanović1 and Dušan Urošević1
1
ARI SERBIA, Small Grains Research Centre, Kragujevac, Serbia & Montenegro
2
Agricultural Faculty, Lešak, University of Priština, Serbia & Montenegro
e-mail: [email protected]
(Received April 3, 2006)
ABSTRACT. The influence of seed maturity on seedling dry matter, efficiency of
seed weight and variability of seedling dry matter in wheat were investigated. The
experiment was performed in semi-controlled conditions in greenhouse. The seeds from
four maturity stages (milk, early dough, full dough and full physiological maturity) were
grown at microtrial experiment in pots with quartz sand at four replications. Dry
matter of seedlings from milk maturity seed ranged from 12.08 mg/ plant (Slavonija) to
18.52 mg/plant (KG-56). Results of seedlings dry matter from seed early dough maturity
(26.86 mg/plant) and full dough maturity (27.62 mg/plant) had shown considerable
higher values than seedlings from seed milk maturity (15.83 mg/plant). In average the
highest value of shoot dry matter had shown seedlings from full dough stage (23.24
mg/plant), which was the same as averaged value for all cultivars and maturity stages
(23.38 mg/plant). The highest root dry matter, in average, was found in seedlings from
full dough maturity (24.93 mg/plant), and the lowest in seedlings from milk maturity
(15.01 mg/plant). The efficiency of seed weight (seedling weight/seed weight) differed in
dependence of cultivar and seed maturity, and ranged from 1.19 (Srbijanka) to 1.56
(Zagrebčanka). In average the highest efficiency of seed weight established in milky
stage (1.61), and decreased to full phisiological stage (1.14). The analysis of variance
was shown that differences of dry matter content of sidlings were highly significant
for cultivar, maturity stage and interaction cultivar x maturity stage. The highest
impact in total variance for dry matter content of sidlings had variance for the
maturity stage (58.05 %), than variance for the cultivar (11.45 %), and the lowest
variance for interaction (9.45 %). Variance for both maturity stage and cultivar was
higher for seedling shoot (65.27 %, 16.05 %, respectively) than for seedling root (46.10
%, 9.89 %, respectively).
INTRODUCTION
The rate of seedlings growth or seedling vigor in cereals is reported to be influenced
by seed size, seed protein content, germination resistance and genotype (RIES &
EVERSON, 1973). The relative importance of these factors is of significance if seedling
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vigor is to be used as a selection criterion for yield improvement in cereal breeding
(EVANS & BHATT, 1977a). The seedling stage begins with the appearance of the first
leaf and ends with the emergence of the first tiller. Up to six seminal roots and three leave
support the plant at this stage. The crown of the plant usually becomes noticeably distinct
after the third leaf has emerged.
The seed quality of wheat is depends of maturity stage and agroecological conditions.
For realisation good sed quality it need harvest in the moment of favorable seed maturity
stage. Harvesting after full physiological stage causes reduce seed yield and seed quality of
wheat (PUCARIĆ & UJEVIĆ 1986). There are many ways of characterizing seed quality,
but the main indicators of quality are seed size and seed efficiency. These two seed quality
traits of wheat are in positive correlations with seedling vigor and growing in the
beginning of the growing.
Early seed formation occurs during the milk stage. The developing endosperm starts
as a milky fluid that increases in solids as the milk stage progresses. Seed size increases
rapidly during this stage. Seed formation is completed during the dough development
stage. The seed accumulates most of its dry weight during dough development. The
transport of nutrients from the leaves, stems, and spike to the developing seed is completed
by the end of the hard dough stage. The developing kernel is physiologically mature at the
hard dough stage even though it still contains approximately 30 percent water. The seed
loses moisture, and any dormancy it may have had, during the ripening stage.
The aim of this study was to examine influence of seed maturity to seedling dry
matter, and seedling vigor, particularly efficiency of seed weight from different seed
maturity in divergent wheat cultivars.
MATERIAL AND METHODS
Winter wheat cultivars (KG-56, Srbijanka, Lepenica, Ljubičevka, Oplenka,
Jugoslavija, Nizija, Slavonija, Zagrebčanka and Baranjka) were grown on experimental
field at Small Grains Research Centre, Kragujevac. The stage of maturity determined with
grain moisture content, color and grain consistency (PAVLIČIĆ, 1964). The stages of
maturity was described previusly (ZEČEVIĆ et al., 2005).
The spike was hand-treshing and grains dried on room temperature to dry air
condition. The wheat seeds from the various stages of maturity (milky, early dough, full
dough and full physiological) were grown at microtrial experiment and were sown in pots
with quartz sand. The seedlings vigour was determined by method EVANS & BHATT
(1977a). Method is consisting in following: fifty seeds of ten cultivars were sown in
microtrial experiment in semi-controlled conditions in greenhouse in pots quartz with sand
at four replications. The pots with seedlings were regular watered. Seedlings were
harvested twenty seven days after planting. Seedlings were uprooted carefuly and washed
in tap water to remove sand particles and blotted dried. Than samples were dried in dryer
at 75o C whole to constant mass. The dry seedlings or shoots were weighed and the data
subjected to an analysis of variance. Efficiency of grain weight was calculated as a ratio of
dry weight of seedling/dry weight of grain.
The following parameters were computed: the average value (⎯x); the coefficient of
variation (V) as an index of relative variability of the trait, and the significant differences
between the averages values were estimated by LSD-test (HADŽIVUKOVIĆ, 1991). The
analysis of variance was performed according to a random block design with two factors.
Components of variance was calculated by allowing the calculation of the components of
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variance (σ2g-genetic, σ2gl-interaction; σ2E-environment; σ2f-phenotypic) by FALCONER
(1981).
RESULTS AND DISCUSSION
Shoot dry matter
The results of seedlings shoot dry matter are displayed in Table 1. According to the
results shoot dry matter in seedlings depended significantly of seed maturity stage. Seedlings
from seed milky maturity ranged from 12.08 mg/ plant (Slavonija) to 18.52 mg/plant (KG56). Results of seedlings from seed dough maturity (26.86 mg/plant; 27.62 mg/ plant) had
shown considerable higher values of dry matter than seedlings from seed milky stage (15.83
mg/plant). In average the highest value of dry matter had shown seedlings from full dough
stage (23.24 mg/plant), which was the same as averaged value for all cultivars and maturity
stages (23.38 mg/plant). In average all cultivars had shoot dry matter above 20 mg/plant,
except Slavonija (17.57 mg/plant).
Table 1. Seedling dry matter of shoot in wheat (mg/plant)
Cultivar
Milk
Early dough
KG-56
18.52
29.23
Srbijanka
14.92
Lepenica
Growth stages
Full dough
Average
33.82
Full
physiological
25.37
26.73
27.69
26.79
23.26
23.06
16.77
29.05
25.26
23.62
23.68
Oplenka
16.52
31.63
31.27
24.71
26.03
Ljubičevka
16.61
26.42
28.89
24.00
23.98
Jugoslavija
14.72
23.75
27.14
19.26
21.22
Zagrebčanka
14.99
22.36
23.85
21.85
20.76
Nizija
15.73
28.13
28.44
30.92
25.80
Slavonija
12.08
21.15
22.53
14.53
17.57
Baranjka
17.40
29.21
28.24
24.92
24.95
Average
15.83
26.86
27.62
23.24
23.38
Root dry matter
Root dry matter depended significantly of cultivar and seed maturity (Table 2). The
results have shown that seedling root dry matter from milky seed maturity ranged from 12.74
mg/plant (Baranjka) to 18.64 mg/plant (Oplenka). The highest root dry matter, in average,
was found in seedlings from full dough maturity (24.93 mg/plant), and the lowest in seedlings
from milk maturity (15.01 mg/plant). Root dry matter in seedlings from full dough maturity
differed significantly than root dry matter in seedlings from full physiological maturity. Also,
differences between root dry matter in seedlings from milky and other stages were highly
significant. In average for seedlings from all maturity stages the highest root dry matter had
KG-56 (26.20 mg/plant), and the lowest Baranjka (18.35 mg/plant). Seed maturity influenced
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significantly on shoot and root dry matter. Dry matter in both (root and shoot) increased from
milky to full dough maturity stage. The lowest shoot and root dry matter in seedlings from
seed milky stage can be explained by low seed weight and low reserve in seed endosperm to
produce roots and shoots. Plants from milky maturity seed in the beginning have poor
growing because of low reserve in seed endosperm, but in the late growing its make up
plants from mature seed (BABAYAN, 1960). Seeds from dough mature produced vigorously
seedlings with higher dry matter because of higher seed weight and higher reserve in seed
endosperm (BOYD et al., 1971; PUCARIĆ & UJEVIĆ, 1986); especially of higher nitrogen
content in large seed because of intensity of dry matter accumulation in wheat seedling is
mainly depending of nitrogen content in seed (PANTIĆ & ĐOKIĆ, 1975). The results of
dry matter of seedlings from dough and full maturity seeds agree with previous studies
(YASEEN et al., 2004).
Table 2. Seedling dry matter of root in wheat (mg/plant)
Cultivar
Seed growth stages
Milk
Early dough
Full dough
KG-56
17.10
28.68
Srbijanka
15.60
Lepenica
Average
32.92
Full
physiological
26.09
26.20
26.05
21.56
19.60
20.70
15.26
23.88
20.76
20.96
20.22
Oplenka
18.64
24.90
23.64
17.94
21.28
Ljubičevka
13.63
19.35
33.97
25.07
23.00
Jugoslavija
13.46
20.01
19.40
22.05
18.73
Zagrebčanka
13.26
17.66
25.76
20.35
19.26
Nizija
16.40
25.50
24.79
28.37
23.76
Slavonija
13.97
22.41
26.98
19.91
20.82
Baranjka
12.74
20.30
19.49
20.86
18.35
Average
15.01
22.87
24.93
22.12
21.23
The maturity stage has high influence to intensity of seedling growing (seedling
vigor). Seed from early growth stages give not so much well grown seedling because of less
dry matter content in seed. Seedlings which produced from later seed maturity stage were
vigorously as a result of more dry matter content in seed. Plants which arise from large seed
show better early vigor as a result of more dry matter content in large seed.
The efficiency of seed weight (seedling weight/seed weight) differed in dependence
of cultivar and seed maturity (Table 3), and ranged from 1.19 (Srbijanka) to 1.56
(Zagrebčanka). In average the highest efficiency of seed weight established in milky stage
(1.61), and decreased to full physiological stage (1.14). Efficiency of seed weight
decreased from milky seed maturity to full physiological seed maturity. It can be explain
because of that smaller seed has higher effect to production of seedling dry matter, what
agree with previous studies (PANTIĆ & ĐOKIĆ, 1975; ĐOKIĆ & LOMOVIĆ, 1988;
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LOMOVIĆ et al., 1994). Other authors are reported that rate of seedlings growth or
seedling vigor in cereals is reported to be influenced by seed size, seed protein content,
germination resistance and genotype (RIES & EVERSON, 1973; EVANS & BHATT,
1977a; 1977b).
Table 3. Efficiency of seed weight (dry weight of seedling/dry weight of seed) in wheat
Cultivar
Seed growth stages
Milk
Early dough
Full dough
KG-56
1.62
1.29
Srbijanka
1.48
Lepenica
Average
1.33
Full
physiological
1.08
1.33
1.30
1.07
0.92
1.19
1.54
1.36
1.13
1.18
1.30
Oplenka
1.93
1.36
1.04
0.94
1.32
Ljubičevka
1.62
1.13
1.22
1.04
1.25
Jugoslavija
1.55
1.16
1.07
1.00
1.20
Zagrebčanka
1.45
1.90
1.42
1.46
1.56
Nizija
1.46
1.47
1.30
1.41
1.41
Slavonija
1.28
1.95
1.27
0.99
1.37
Baranjka
2.13
1.21
1.36
1.33
1.51
Average
1.61
1.41
1.22
1.14
1.38
The seedling dry matter variabilty
The analysis of variance was shown that differences of dry matter content of sidlings
were highly significant for cultivar, maturity stage and interaction cultivar x maturity stage
(Table 4, 5 and 6). The highest impact in total variance for dry matter content of sidlings
had variance for the maturity stage (58.05 %), than variance for the cultivar (11.45 %).
Higher variance for maturity stage (65.27 %) and cultivar (16.05 %) were established for
seedlings shoot than for seedlings root (46.10 %, 9.89 %, respectively). Seed maturity had
higher influence to variability of seedlings dry matter than cultivar. Higher variability for seed
maturity can be explain by different seed weight or seed size in different maturity stages what
is in agreement with results obtained by EVANS & BHATT (1977) who have shown that
fifty-three percent of the variation in seedling vigor was attributed to variation in seed size
and protein content. According to the results variation coefficient for seedlings was 12.78 %.
It was established higher variation coefficient for the root dry matter (15.03) than for the
shoot dry matter (10.21), what agree with investigation obtained by QAYYUM KHAN et al.
(2002).
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Table 4. Phenotypic variance of seedling dry matter of wheat
Source of
DF
variation
Replication
Cultivar
Maturity
stage
MS
Ft
3
53.257
-
9
379.455
11.49**
3
3733.220
Cultivar x
maturity
27
stage
Error
117
Total
159
113.08*
*
Components of
variance
LSD
%
δ²
Cultivar
Maturity
17.95 11.45
stage
Cultivar x
91.02 58.05
maturity
stage
92.292
2.80**
14.82
9.45
33.013
-
-
33.01
156.8
21.05
100.0
0.01
0.05
5.32
4.02
3.36
2.54
10.6
4
8.05
V=12.78 %
Table 5. Phenotypic variance of seedling shoots dry matter of wheat
Source of
DF
variation
Replication
Cultivar
Maturity
stage
MS
Ft
3
23.283
-
9
128.131
22.50
3
1159.986 203.69
Cultivar x
maturity
27
stage
Error
117
Total
159
Components of
variance
LSD
%
δ²
Cultivar
Maturity
7.03
16.05
stage
Cultivar x
28.61 65.27
maturity
stage
15.673
2.75
2.49
5.68
5.695
-
-
5.70
43.83
13.00
100.00
0.01
0.05
2.21
1.67
1.40
1.06
4.42
3.34
V=10.21%
Table 6. Phenotypic variance of seedling root dry matter of wheat
Source of
DF
variation
Replication
Cultivar
Maturity
stage
Components of
variance
LSD
%
δ²
Cultivar
Maturity
3.69
9.89
stage
Cultivar x
17.21 46.10
maturity
stage
MS
Ft
3
7.908
-
9
94.325
9.29**
3
723.837
71.31**
35.277
3.48**
6.28
16.82
10.150
-
-
10.15
37.33
27.91
100.00
Cultivar x
maturity
27
stage
Error
117
Total
159
V=15.03 %
0.01
0.05
2.95
2.23
1.86
1.41
5.90
4.46
171
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