2009 - GRAQ

Transcrição

2009 - GRAQ

RREEELLLAAATTTÓÓÓRRRIIIOOO DDDEEE AACCCTTTIIIVVVIIIDDDAAADDDEEESSS
22000099
GRAQ – Grupo de Reacção e Análises Químicas
ANNUAL REPORT
20 0 9
REQUIMTE-ISEP
Rua Dr. António Bernardino de Almeida, 431
4200-072 Porto
Portugal
tel.:+351-228340500
INDEX
BRIEF HISTORY
3
MEMBERS
4
A.
RESEARCH AREAS
7
1.
ANALYTICAL CHEMISTRY
7
1.1.
SUB-AREAS IN ANALYTICAL CHEMISTRY
1.1.1. Quality control and authenticity of food products
1.1.2. Environmental analysis
1.1.3. Health and pharmaceutical analysis
8
8
10
11
1.2.
OUTPUT INDICATORS
1.2.1. FCT funded projects
1.2.2. non-FCT funded projects
1.2.3. Papers (ISI)
1.2.4. Papers (non-ISI)
1.2.5. Books
1.2.6. Ph.D. theses
1.2.7. MSc theses
1.2.8. International conferences
1.2.9. National conferences
13
13
14
14
15
15
15
15
16
19
1.3.
OTHER ACTIVITIES
20
1.4.
FUTURE RESEARCH
22
2.
ENVIRONMENTAL CONTROL AND REMEDIATION
23
2.1.
SUB-AREAS IN ENVIRONMENTAL CONTROL AND REMEDIATION
2.1.1. Waste management and toxicological evaluation
2.1.2. Soil and groundwater remediation
2.1.3. Removal of toxic compounds by means of adsorption strategies
24
24
24
25
2.2.
OUTPUT INDICATORS
2.2.3. Papers (ISI)
2.2.5. Books
2.2.6. Ph.D. theses
2.2.7. MSc theses
26
26
26
26
27
27
27
27
27
29
2.3.
OTHER ACTIVITIES
29
2.4.
FUTURE RESEARCH
30
3.
RESEARCH METRICS
31
3.1.
OUTPUT INDICATORS
31
31
B.
32
SCIENTIFIC EVALUATION
GRAQ – ANNUAL REPORT – 2009
BRIEF HISTORY
BRIEF HISTORY
The “Grupo de Reacção e Análises Químicas” (GRAQ) was formed in January 1999 by researchers from
the Instituto Superior de Engenharia do Porto (ISEP), and is located on the campus of ISEP. In 1999 the
group joined the Centro de Química da Universidade do Porto (CEQUP).
GRAQ became part of REQUIMTE in 2000 through the partnership of CEQUP with the Centro de
Química Fina e Biotecnologia (CQFB) da Faculdade de Ciências e Tecnologia da Universidade Nova de
Lisboa.
REQUIMTE is the largest network in Chemistry and Chemical Engineering established in Portugal and
was recognized as the Laboratório Associado para a Química Verde (Green Chemistry) by the
Portuguese Ministério da Ciência e do Ensino Superior in November 2001.
The objectives of REQUIMTE are:
a)
To encourage the use of clean products and technologies;
b)
To assist industry in the design and implementation of non-aggressive chemical processes;
c)
To train young researchers in interdisciplinary areas related with the practice of sustainable
chemistry;
d)
To publicise the principles of Green Chemistry and to alert society for the necessity of a
sustainable practice in everyday life.
Research is presently focused in the following thematic areas of: i) natural products, (ii) food quality and
safety, (iii) clean production technologies and processes, (iv) environmental control and remediation and
(v) catalysts, solvents and non-toxic compounds.
The sharing of multidisciplinary scientific knowledge, technology and equipment between researchers of
the two centres that form the network, has significantly contributed to the development of new projects in
Green Chemistry and to the enrichment and training of graduate students by making easier the mobility of
human resources.
At present the network REQUIMTE can be described as a big Laboratory that has two operating sites,
one at the Universidade Nova de Lisboa and the other at the Universidade do Porto
In this concept, the GRAQ research lines mainly focus on Analytical Chemistry and Environmental
Control and Remediation.
Scientific Coordinator: Cristina Maria Fernandes Delerue Alvim de Matos ([email protected])
Internet: www.graq.isep.ipp.pt | www.requimte.pt
[3]
MEMBERS
MEMBERS
Permanent Members
1.
Ph.D.
Cristina Maria Fernandes Delerue Alvim de Matos
Professor Coordenador (ISEP)
2.
Ph.D.
Maria do Carmo Veiga Fernandes Vaz
Professor Coordenador (ISEP)
3.
Ph.D.
Maria Teresa Pereira de Oliva Teles Moreira
Professor Adjunto (ISEP)
4.
Ph.D
Maria Conceição Carvalho Benta de Oliveira Neves
Professor Adjunto (ISEP)
5.
Ph.D.
Maria Goreti Ferreira Sales
eq. Professor Adjunto (ISEP)
6.
Ph.D.
Maria Manuela Barbosa Correia
7.
Ph.D.
Sónia Adriana Ribeiro da Cunha Figueiredo
8.
Ph.D.
Simone Barreira Morais
9.
Ph.D.
Susana Maria Ribeiro e Sousa Mendes de Freitas
10.
Ph.D.
Valentina Maria Fernandes Domingues
11.
Ph.D.
Florinda Figueiredo Martins
eq. Assistante (ISEP)
12.
Ph.D.
Hendrikus Petrus Antonius Nouws
13.
Ph.D.
Olga Manuela Matos de Freitas
14.
Ph.D.
Subramanian Viswanathan
Auxiliary Investigator (REQUIMTE)
15.
MSc
Abel José Assunção Duarte
16.
MSc
Maria João Dantas Ramalhosa Ferreira
eq. Assistante (ISEP) (50% FFUP)
17.
MSc
Salomé Sousa Teixeira
18.
MSc
José Tomás Veiga Soares de Albergaria
Técnico Superior (ISEP)
19.
MSc
Maria Aurora Soares da Silva
20.
MSc
Bruno José Rocha Pereira
21.
MSc
Sérgio Alberto Cruz Monteiro de Morais
22.
MSc
Paula Celeste Baptista Paíga
Técnico Superior 2ª Classe (REQUIMTE)
23.
BEng
Maria Isabel Viana de Brito Limpo de Serra
[4]
MEMBERS
MEMBERS
Non-permanent members
Ph.D. students
1.
BEng
Díonisia Maria Oliveira Castro
2.
MSc
Maria de Fátima de Sá Barroso
50% FFUP
FCT Grant: SFRH/BD/29440/2006
3.
MSc
Sofia Alexandra Alves Almeida
50% FFUP
4.
MSc
Marta Maria Pereira da Silva Neves
5.
BEng
Mónica Alexandra Oliveira Dias Teixeira
FCT Grant: n/a
6.
Msc
Antonio Vega Y de la Fuente
7.
MSc
Maria Manuela Martins de Carvalho
FCT Grant: n/a
8.
MSc
Virgínia Maria Monteiro Cruz Fernandes
9.
MSc
Raquel Barbosa Queirós
75% FCUP
10.
BSc
Débora Liliana Gutierres M. F. Soares
50% FFUP
75% FEUP
FCT Grant: n/a
MSc students
1.
Ana Catarina Dias Vieira da Silva
FFUP
2.
Ana Isabel Ribeiro de Pinho
ISEP
3.
Bie van der Vliet
Karel de Grote Hogeschool (BE)
4.
Carla Alexandra Moreira Vieira
ISEP
5.
Cleusa Lopes da Luz
ISEP
6.
Diana Margarida Ferreira Pinheiro
ISEP
7.
Diana Paula Cunha de Aguilar de Azevedo Machado
ISEP
8.
Elisa Alexandra Mota Ferreira
FMUP
9.
Fernando Odeberto Gonçalves Pereira
ISEP
10.
Helena Maria Vaz de Oliveira
ISEP
11.
Joana Alves Ferreira da Cruz
ISEP
12.
Joana Gomes Martins
ISEP
13.
Joana Maria Cesar Veloso Ferreira
ISEP
14.
José Alberto Nunes da Silva Ferreira
FCUP
15.
José António Sousa
FEUP
16.
José Camilo Carvalinho Sousa Pinto
ISEP
17.
Magda Cristina Teixeira de Passos Guimarães
University of Southern Denmark
18.
Maria Adelaide Rocha de Sousa
ISEP
19.
Maria Isabel Mesquita Guimarães Seguro Pereira de Soares FCUP
20.
Marta Gonçalves Varanda
ISEP
21.
Marta Madalena Marques Oliveira
FFUP
22.
Nuno Miguel Simões Leal Ribeiro
ISEP
23.
Susana Natércia Oliveira Ribeiro
ISEP
24.
Tânia Sofia Cardoso Ribeiro Rebelo
ISEP
[5]
MEMBERS
MEMBERS
Non-permanent members (cont.)
Grant holders
1.
MSc
Joana Rafaela Lara Guerreiro
FCT project: PTDC/AGR-AAM/68359/2006
2.
MSc
Felismina Teixeira Coelho Moreira
FCT project: PTDC/AGR-AAM/68359/2006
3.
MSc
Mónica Rosas da Silva
FCT project: PTDC/ECM/68056/2006
4.
MSc
António Carlos Alves Soares
FCT project: PTDC/ECM/68056/2006
5.
--
Pedro Romeu da Silva Soares
FCT project: BII 2008-2009
6.
--
Adão Batista Martins Pinto
7.
--
Ana Raquel dos Reis Ferreira
8.
--
Laura Cristina Oliveira Sousa
9.
--
Liliana Adelina Afonso N. Almeida Truta
10.
--
Isa de Sousa Matos
11.
..--
Marcela de Jesus da Cunha Oliveira
12.
..--
Isabel Patrícia Ribeiro Moreira
13.
..--
Irene Cristina de Sousa Azevedo
[6]
RESEARCH AREAS – ANALYTICAL CHEMISTRY
A. RESEARCH AREAS
1.
ANALYTICAL CHEMISTRY
TEAM MEMBERS
Permanent Members
Cristina Maria Delerue-Matos
Maria do Carmo Veiga Fernandes Vaz
Maria Manuela Barbosa Correia
Simone Barreira Morais
Susana Maria Ribeiro e Sousa Mendes de Freitas
Subramanian Viswanathan
Abel José Assunção Duarte
Maria João Dantas Ramalhosa Ferreira
Salomé Sousa Teixeira
MSc
Ana Catarina Dias Vieira da Silva
Grant holders
Joana Rafaela Lara Guerreiro
Felismina Teixeira Coelho Moreira
Laura Cristina Oliveira Sousa
Liliana Adelina Afonso N. Almeida Truta
Isa de Sousa Matos
Marcela de Jesus da Cunha Oliveira
Isabel Patrícia Ribeiro Moreira
Ph.D.
Dionísia Maria Oliveira Castro
Maria de Fátima de Sá Barroso
Sofia Alexandra Alves Almeida
Marta Maria Pereira da Silva Neves
Mónica Alexandra de Oliveira Dias Teixeira
Virgínia Maria Monteiro da Cruz Fernandes
Raquel Barbosa Queirós
Débora Liliana Gutierres Mirones F. Soares
Ana Isabel Ribeiro de Pinho
Cleusa Lopes da Luz
Elisa Alexandra Mota Ferreira
Fernando Odeberto Gonçalves Pereira
Helena Maria Vaz de Oliveira
Joana Gomes Martins
José Alberto Nunes da Silva Ferreira
José António Sousa
José Camilo Carvalinho Sousa Pinto
Maria Adelaide Rocha de Sousa
Maria Isabel Mesquita G. Seguro Pereira de Soares
Marta Madalena Marques Oliveira
Tânia Sofia Cardoso Ribeiro Rebelo
OUTPUT INDICATORS (SUMMARY)
FCT funded projects
non-FCT funded projects
Papers (ISI)
Papers (non-ISI)
Books
Ph.D. theses
MSc theses
Conferences (international)
Oral presentations
Poster presentations
Proceedings papers
Conferences (national)
Oral presentations
Poster presentations
Proceedings papers
Conferences (organization)
3
4
13
1
--13
2
33
1
-13
4
1
[7]
The purpose of analytical chemistry is to answer the following questions: which elements and/or
compounds are present in a sample (qualitative analysis) and in which concentration (quantitative
analysis)? There is a wide variety of analytical techniques available to answer these questions.
Historically the determinations were based on direct measurements of mass and/or volume. Nowadays
the majority of determinations are based on the measurement of a large variety of properties, like, optical,
piezoelectrical, thermal, and electrochemical.
Some recent trends in analytical chemistry are the automatization and miniaturization of measuring
devices in order to speed up the analysis time and to allow point-of-care analysis. As in many areas,
nanotechnology has also been introduced in this field of study.
One of the most promising and rapidly growing areas in analytical chemistry is the development and use
of sensors based on chemical and/or biochemical mechanisms. In the construction of a sensor a selective
recognition element is coupled to a transducer. Nanotecnological approaches for the construction of the
transducer can significantly improve the selectivity and sensitivity of the analysis and greatly reduce the
measuring device. The term biosensor is used when the sensing layer is based on biochemical
recognition/binding elements. Electrochemical biosensors are used in point-of-care devices since they are
portable, simple, easy to use, cost effective and in most cases disposable (for example for the
measurement of blood glucose). The electrochemical instruments used with the biosensors have also
been miniaturized to small pocket size devices which make them applicable for home use or the doctor’s
office.
Besides the development of the analytical technique itself, sample preparation has also received much
attention, particularly regarding solid-phase- and microwave-aided extractions.
1.1. SUB-AREAS IN ANALYTICAL CHEMISTRY
In the subsequent sections a summary of several achievements in analytical chemistry in 2009 are
presented, for further reading the consultation of the published papers is recommended.
1.1.1. Quality control and authenticity of food products
Concentrations of eleven trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) were measured
in 39 (natural and flavoured) water samples. Determinations were performed using graphite furnace
electrothermetry for almost all elements (Al, As, Cd, Cr, Co, Mn, Ni, Pb, and Si). For Se determination
hydride generation was used, and cold vapour generation for Hg. These techniques were coupled to
atomic absorption spectrophotometry. The trace element content of still or sparkling natural waters
changed from brand to brand. Significant differences between natural still and natural sparkling waters
(p < 0.001) were only apparent for Mn. The Mann–Whitney U-test was used to search for significant
differences between flavoured and natural waters. The concentration of each element was compared with
the presence of flavours, preservatives, acidifying agents, fruit juice and/or sweeteners, according to the
labelled composition. It was shown that flavoured waters generally increase the trace element content.
[8]
The addition of preservatives and acidifying regulators had a significant influence on Mn, Co, As and Si
contents (p < 0.05). Fruit juice can also be correlated to the increase of Co and As. Sweeteners did not
provide any significant difference in Mn, Co, Se and Si content.
Macro (Ca, Mg, K, Na) and micromineral (Fe, Zn, Cu) composition of 39 waters was analysed.
Determinations were made by atomic flame spectrophotometry for macrominerals and electrothermic
atomization in graphite furnace for microminerals. Mineral contents of still or sparkling natural waters
(without flavours) changed from brand to brand. Mann–Whitney test was used to search for significant
differences between flavoured and natural waters. For that, the concentration of each mineral was
compared to the presence of flavours, preservatives, acidifying agents, fruit juice and/or sweeteners,
according to the labelled composition. The statistical study demonstrated that flavoured waters generally
have increased contents of K, Na, Fe and Cu. The added preservatives also led to significant differences
in the mineral composition. Acidifying agents and fruit juice can also be correlated to the increase of Mg,
K, Na, Fe and Cu. Sweeteners do not provide any significant difference in Ca, Mg, Fe and Zn contents.
QuEChERS method was evaluated for extraction of 16 PAHs from fish samples. For a selective
measurement of the compounds, extracts were analysed by LC with fluorescence detection. The overall
analytical procedure was validated by systematic recovery experiments at three levels and by using the
standard reference material SRM 2977 (mussel tissue). The targeted contaminants, except naphthalene
and acenaphthene, were successfully extracted from SRM 2977 with recoveries ranging from 63.5 –
110.0 % with variation coefficients not exceeding 8 %. The optimum QuEChERS conditions were the
following: 5 g of homogenised fish sample, 10 mL of ACN, agitation performed by vortex during 3 min.
Quantification limits ranging from 0.12 – 1.90 ng/g wet weight (0.30 – 4.70 µg/L) were obtained. The
optimized methodology was applied to assess the safety concerning PAHs contents of horse mackerel
(Trachurus trachurus), chub mackerel (Scomber japonicus), sardine (Sardina pilchardus) and farmed
seabass (Dicentrarchus labrax). Although benzo(a)pyrene, the marker used for evaluating the
carcinogenic risk of PAHs in food, was not detected in the analysed samples (89 individuals
corresponding to 27 homogenized samples), the overall mean concentration ranged from 2.52 ±
1.20 ng/g in horse mackerel to 14.6 ± 2.8 ng/g in farmed seabass. Significant differences were found
between the mean PAHs concentrations of the four groups.
An analytical method, based on microwave-assisted extraction and LC with diode array detection, for the
determination of six carbamate and three urea pesticides in fresh and processed tomato samples is
described. Significant parameters affecting extraction efficiency were optimized. Under optimum
microwave-assisted extraction conditions (20 mL acetonitrile, for 10 minutes, at 60 ºC), pesticides were
extracted with recoveries ranging from 57.6 to 102% (RSD < 7%). Quantification limits between 6.5 and
39.6 µg/kg were obtained. A total number of 28 different fresh tomato samples and 6 processed tomato
products were analysed. Confirmation of suspicious samples was performed by LC-MS.
[9]
An analytical multiresidue method for the simultaneous determination of seven pesticides in fresh
vegetable samples, namely, courgette (Cucurbita pepo), cucumber (Cucumis sativus), lettuce (Lactuca
sativa, Romaine and Iceberg varieties) and peppers (Capsicum sp.) is described. The procedure, based
on microwave-assisted extraction (MAE) and analysis by liquid chromatography–photodiode array (LC–
PDA) detection was applied to four carbamates (carbofuran, carbaryl, chlorpropham and EPTC) and
three urea pesticides (monolinuron, metobromuron and linuron). Extraction solvent and the addition of
anhydrous sodium sulphate to fresh vegetable homogenate before MAE were the parameters optimised
for each commodity. Recovery studies were performed using spiked samples in the range 250 –
403 µg/kg in each pesticide. The pesticide residues were extracted using 20 mL acetonitrile at 60 ºC, for
10 min. Acceptable recoveries and RSDs were attained (overall average recovery of 77.2 % and RSDs
are lower than 11 %). Detection limits ranged between 5.8 µg/kg for carbaryl to 12.3 µg/kg for carbofuran.
The analytical protocol was applied for quality control of 41 fresh vegetable samples bought in Oporto
Metropolitan Area (North Portugal). None of the samples contained any detectable amounts of the
studied compounds.
A flow-spectrophotometric method is proposed for the routine determination of tartaric acid in wines. The
reaction between tartaric acid and vanadate in acetic media is carried out in flowing conditions and the
subsequent colored complex is monitored at 475 nm. The stability of the complex and the corresponding
formation constant are presented. The effect of wavelength and pH was evaluated by batch experiments.
The selected conditions were transposed to a flow-injection analytical system. Optimization of several
flow parameters such as reactor lengths, flow-rate and injection volume was carried out. Using optimized
conditions, a linear behavior was observed up to 1000 μg mL-1 tartaric acid, with a molar extinction
coefficient of 450 L/mg.cm and ± 1 % repeatability. Sample throughput was 25 samples per hour. The
flow-spectrophotometric method was satisfactorily applied to the quantification of tartaric acid (TA) in
wines from different sources. Its accuracy was confirmed by statistical comparison to the conventional
Rebelein procedure and to a certified analytical method carried out in a routine laboratory.
DNA-based biosensors are developed after immobilization and electrooxidation of guanine and adenine
on a glassy carbon electrode. Both the guanine and the adenine biosensors are employed for the
voltammetric detection of antioxidant capacities of flavoured waters. The method relies on monitoring the
changes of the intrinsic anodic response of the surface-confined guanine and adenine species, resulting
from its interaction with free radicals from Fenton-type reaction in absence and presence of antioxidant.
Ascorbic acid is used as standard to evaluate antioxidant capacities of samples.
1.1.2. Environmental analysis
A methodology based on microwave-assisted extraction (MAE) and LC with fluorescence detection (FLD)
was investigated for the efficient determination of 15 polycyclic aromatic hydrocarbons (PAHs) regarded
as priority pollutants by the US Environmental Protection Agency and dibenzo(a,l)pyrene in atmospheric
[ 10 ]
particulate samples. PAHs were successfully extracted from real outdoor particulate matter (PM) samples
with recoveries ranging from 81.4 ± 8.8 to 112.0 ± 1.1 %, for all the compounds except for naphthalene
(62.3 ± 18.0 %) and anthracene (67.3 ± 5.7 %), under the optimum MAE conditions (30.0 mL of ACN for
20 min at 110 ºC). No clean-up steps were necessary prior to LC analysis. LOQs ranging from
3
3
0.0054 ng/m for benzo(a)anthracene to 0.089 ng/m for naphthalene were reached. The validated MAE
methodology was applied to the determination of PAHs from a set of real world PM samples collected in
Oporto (north of Portugal). The sum of particulate-bound PAHs in outdoor PM ranged from 2.5 and
3
28 ng/m .
Seven pyrethroids (bifenthrin, fenpropathrin, k-cyhalothrin, permethrin, a-cypermethrin, fenvalerate, and
deltamethrin) were extracted from water using C18 solid-phase extraction disks, followed by gas
chromatography with an electron capture detector (GC-ECD) analysis. The limits of detection in water
samples ranged from 0.5 ng/L (fenpropathrin) to 110 ng/L (permethrin), applying the calibration graph.
The effects of different numbers of (re)utilizations of the same disks (up to four times with several
concentrations) on the recoveries of the pyrethroids were considered. The recoveries were all between 70
and 120 % after four utilizations of the same disk. There was no difference between these recoveries at a
confidence level of 95 %.
The industrial manufacturing of metallic objects results in a high level of foundry waste sands that may
contain toxic compounds such as formaldehyde. The formaldehyde content of foundry waste sands was
evaluated by liquid chromatography. Samples were collected during various steps of the industrial
processes. Results showed that the phenolic alkaline process generated waste sands with higher
-3
formaldehyde content than the furanic process; the highest value was 7.6×10
% (w/w). In this work,
formaldehyde content decreased with time in all of the samples studied, revealing that most formaldehyde
was released to the occupational environment. Also research is done to assess the evidence for organic
pollutants and heavy metals in aquatic environment and food and the development of methodologies
concerning the quantification of these compounds that exists in extremely low concentration and, in
future, establish a monitoring and controlling program for them. Target analytes will be selected on the
basis of their presumable presence in the water matrices and their environmental significance and include
compounds of different sources of contamination: estrogens (domestic), pesticides (agriculture), heavy
metals and plasticizers (industrial).
1.1.3. Health and pharmaceutical analysis
Because of the mutagenic and/or carcinogenic properties, Polycyclic Aromatic Hydrocarbons (PAH), have
a direct impact on human population. Consequently, there is a widespread interest in analysing and
evaluating the exposure to PAH in different indoor environments, influenced by different emission
sources. The information on indoor PAH is still limited, mainly in terms of PAH distribution in indoor
particles of different sizes; thus, this study evaluated the influence of tobacco smoke on PM10 and PM2.5
[ 11 ]
characteristics, namely on their PAH compositions, with further aim to understand the negative impact of
tobacco smoke on human health. Samples were collected at one site influenced by tobacco smoke and at
one reference (non-smoking) site using low-volume samplers; the analyses of 17 PAH were performed by
Microwave Assisted Extraction combined with Liquid Chromatography (MAE–LC). At the site influenced
by tobacco smoke PM concentrations were higher 650 % for PM10, and 720 % for PM2.5. When influenced
by smoking, 4 ring PAH (fluoranthene, pyrene, and chrysene) were the most abundant PAH, with
concentrations 4600 – 21000 % and 5100 – 20800 % higher than at the reference site for PM10 and
PM2.5, respectively, accounting for 49 % of total PAH (SPAH). Higher molecular weight PAH (5–6 rings)
reached concentrations 300 – 1300 % and 140 – 1700 % higher for PM10 and PM2.5, respectively, at the
site influenced by tobacco smoke. Considering 9 carcinogenic PAH this increase was 780 % and 760 %
in PM10 and PM2.5, respectively, indicating the strong potential risk for human health. As different
composition profiles of PAH in indoor PM were obtained for reference and smoking sites, those 9
carcinogens represented at the reference site 84 % and 86 % of SPAH in PM10 and PM2.5, respectively,
and at the smoking site 56 % and 55 % of SPAH in PM10 and PM2.5, respectively. All PAH (including the
carcinogenic ones) were mainly present in fine particles, which corresponds to a strong risk for
cardiopulmonary disease and lung cancer; thus, these conclusions are relevant for the development of
strategies to protect public health.
The purpose of this work was to assess the acute toxicity on male mice to a chromated copper arsenate
(CCA) solution, a widespread wood preservative used in building industry until 2002. Animals were
subcutaneously injected with CCA (7.2 mg/kg arsenic and 10.2 mg/kg chromium per body weight), CrO3
(10.2 mg/kg), As2O5 (7.2 mg/kg) and NaCl (0.9%) per se, during 48 h and 96 h, for histopathology,
histochemistry, chromium and arsenic analysis. The results showed some histopathological changes
within renal tubules lumen of CCA exposed animals (during 48 h, and 96 h), and CrO 3 (for the period of
96 h). Furthermore, the renal levels of arsenic and chromium in treated animals were statistically more
evident than controls. Although, the same contents of pentavalent arsenic and hexavalent chromium were
injected into treated animals with CCA and with the prepared solutions of As 2O5 and CrO3, a different
distribution of the pattern of these compounds was observed in kidneys; the arsenic concentration on
kidneys of CCA-exposed animals was much higher than those in animals exposed to As2O5 (32- and 28fold higher at 14 and 24h, respectively). However, the elimination of chromium seems to occur similarly in
the kidneys of animals treated with CCA and CrO3 per se. Interactions among the components of CCA
result in a marked decrease of the ability of kidney to eliminate simultaneously both analytes. The
nephrotoxicity of CCA was higher than its components per se, evidencing a possible synergetic effect.
New PVC membrane electrodes for the potentiometric determination of sulfadiazine (SDZ) were
developed. The electrodes were fabricated with conventional and tubular configurations with a graphitebased electrical contact, and no internal reference solution. The selective membranes consist of
bis(triphenylphosphoranilidene)ammonium·SDZ (electrode A), tetraoctylammonium bromide (electrodeB),
[ 12 ]
or iron(II)-phthalocyanine (FePC) (electrode C) electroactive materials dispersed in a PVC matrix of onitrophenyl octyl ether (o-NPOE) plasticizer. The sensors A, B, and C displayed linear responses over the
concentration ranges 1.0×10
–2
–5
–2
– 1.0×10 , 1.0×10
–6
–2
– 7.5×10 , and 3.2×10
–6
– 7.0×10
mol/L (detection
limits of 1.09, 2.04 and 0.87 mg/mL) with anionic slopes of –57.3 ± 0.1, –46.7 ± 0.5, and –65.1 ± 0.2 mV
–1
decade , respectively. No effect from pH was observed within 4.0 – 5.5, 4.8 – 10, and 4.5 – 8,
respectively, and good selectivity was found. The sensors were applied to the analysis of
pharmaceuticals and biological fluids in steady state and in flow conditions.
Currently a nanostructured biosensor is being developed for the diagnosis of celiac disease. The
transducer part of the sensor is based on a screen-printed carbon electrode modified with carbon
nanotubes/nanofibers and gold nanoparticles. The sensing layer of the sensor is based an antibodyantigen interaction.
1.2. OUTPUT INDICATORS
Project reference:
Project title:
Duration:
Responsible investigator:
Principal contractor:
Participating institution(s):
Funding (total):
Funding (ICETA):
Project reference:
Project title:
Duration:
Responsible investigador:
Funding (total):
Funding (ICETA):
Project reference:
Project title:
Duration:
Funding (total):
Funding (ICETA):
PTDC/AGR-AAM/68359/2006
Detection and quantification of antimicrobials in fish and in waters from
aquaculture
July 2007 – June 2010
Maria Goreti Fereira Sales
Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares-Porto (ICETAPorto/UP)
Instituto Nacional de Recursos Biológicos, I.P. (INRB/MADRP)
€ 131 553
€ 64 664
PTDC/AGR-ALI/65528/2006
Evaluation of ochratoxin A exposure level of Portuguese population: bread
consumption and urine levels
June 2007 – May 2010
Celeste Matos Lino (Universidade de Coimbra)
Universidade de Coimbra
Instituto Politécnico de Bragança
€ 74 866
€ 12 977
PTDC/QUI/71001/2006
SenRONS - Development of optical fiber sensors for the determination of reactive
oxygen (ROS) and nitrogen (RNS) species in biological systems
January 2008 – December 2010
Joaquim Carlos Gomes Esteves da Silva (Universidade do Porto)
Associação para o Desenvolvimento da Faculdade de Ciências (ADFC/FC/UP)
Universidade de Coimbra (UC)
Instituto de Engenharia de Sistemas e Computadores do Porto (INESC
Porto/FE/UP)
€ 104 200
€ 3 900
[ 13 ]
Project reference:
Project title:
Duration:
Funding source:
Funding (total):
Funding (ICETA):
Project reference:
Project title:
Duration:
Funding source:
Funding (total):
Funding (ICETA):
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Project title:
Duration:
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Funding (total):
Funding (ICETA):
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Funding (ICETA):
CESPU01
Monitorização de triazinas em águas e no homem. Mecanismo patogénico.
Mónica Alexandra de Oliveira Dias Teixeira
CESPU
€ 5 000
€ 5 000
------Protective role of antioxidants at DNA oxidative damage: biosensors, mechanism
and screening
Instituto de Ciências e Tecnologias Agrárias e Agro-Alimentares - Porto (ICETA)
Centro de Química Fina e Biotecnologia da Universidade Nova de Lisboa – CQFB.
REQUIMTE
€ 8 000
€ 4 000
------Avaliação da Contaminação de Águas Residuais Hospitalares
Conceição Montenegro (FFUP)
University of Porto
€ 4 000
€ 4 000
------Componentes da fracção azotada do leite - influência na qualidade e implicações
na saúde dos consumidores
Beatriz Oliveira (FFUP)
University of Porto
€ 4 000
€ 4 000
1.2.3. Papers (ISI)
1. K. Slezakova, D. Castro, M.C. Pereira, S. Morais, C. Delerue-Matos, M.C. Alvim-Ferraz
Influence of tobacco smoke on carcinogenic PAH composition in indoor PM10 and PM2.5
Atmospheric Environment 43 (40) (2009) 6376-6382.
2. M.F. Barroso, S. Ramos, M.T. Oliva-Teles, C. Delerue-Matos, M.G.F. Sales, M.B.P.P. Oliveira
Survey of trace elements (Al, As, Cd, Cr, Co, Hg, Mn, Ni, Pb, Se, and Si) in retail samples of flavoured and
bottled waters
Food Additives and Contaminants Part B - Surveillance 2 (2) (2009) 121-130.
3. Maria João Ramalhosa, Paula Paíga, Simone Morais, Cristina Delerue-Matos, Maria Beatriz Prior Pinto Oliveira
Analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, easy, cheap, effective, rugged, and
safe extraction method
Journal of Separation Science 32 (20) (2009) 3529-3538.
4. Rita Cerejeira Matos, Catarina Vieira, Simone Morais, Maria de Lourdes Pereira, Júlio Pedrosa
Nephrotoxicity effects of the wood preservative chromium copper arsenate on mice: Histopathological and
quantitative approaches
Journal of Trace Elements in Medicine and Biology 23 (3) (2009) 224-230.
[ 14 ]
5. Sandra S.T. Bastos, Paula A.R. Tafulo, Raquel B. Queiros, Cristina D. Matos, M. Goreti F. Sales
Rapid Determination of Tartaric Acid in Wines
Combinatorial Chemistry & High Throughput Screening 12 (7) (2009) 712-722.
6. M. Fátima Barroso, Aurora Silva, Sandra Ramos, M.T. Oliva-Teles, Cristina Delerue-Matos, M. Goreti F. Sales,
M.B.P.P. Oliveira
Flavoured versus natural waters: Macromineral (Ca, Mg, K, Na) and micromineral (Fe, Cu, Zn) contents
Food Chemistry 116 (2) (2009) 580-589.
7. A.H. Kamel, S.A.A. Almeida, M.G.F. Sales, F.T.C. Moreira
Sulfadiazine-Potentiometric Sensors for Flow and Batch Determinations of Sulfadiazine in Drugs and Biological
Fluids
Analytical Sciences 25 (3) (2009) 365-371.
8. V. Domingues, A. Alves, M. Cabral, C. Delerue-Matos
Use and reuse of SPE disks for the determination of pyrethroids in waters by GC-ECD
Analytical Letters 42 (4) (2009) 706-726.
9. M.T. Oliva-Teles, P. Paíga, C.M. Delerue-Matos, M.C.M. Alvim-Ferraz
Evaluation of Formaldehyde in Foundry Waste Sands Using Liquid Chromatography
10. P. Paíga, S. Morais, M. Correia, C. Delerue-Matos, A. Alves
Screening of Carbamates and Ureas in Fresh and Processed Tomato Samples using Microwave-Assisted
Extraction and Liquid Chromatography
11. D. Castro, K. Slezakova, M.T. Oliva-Teles, C. Delerue-Matos, M.C. Alvim-Ferraz, S. Morais, M.C. Pereira
Analysis of Polycyclic Aromatic Hydrocarbons in Atmospheric Particulate Samples by Microwave-assisted
Extraction and Liquid Chromatography
Journal of Separation Science 32 (4) (2009) 501-510.
12. R.C. Matos, C. Vieira, S. Morais, M.L. Pereira, J.P. de Jesus
Nephrotoxicity of CCA-treated wood: a comparative study with As 2O5 and CrO3 on mice
Environmental Toxicology and Pharmacology 27 (2) (2009), 259-263.
13. P. Paíga, S. Morais, M. Correia, C. Delerue-Matos, A. Alves
Determination of Carbamate and Urea Pesticide Residues in Vegetables Using Microwave-assisted Extraction
and Liquid Chromatography
International Journal of Environmental Analytical Chemistry 89 (3) (2009), 199-210.
1. Correia, M., Delerue-Matos, C., Beatriz Oliveira
Avaliação de nitratos e nitritos em vegetais
Revista de Segurança e Qualidade Alimentar, N. 7 (2009), 14-16.
1.2.5. Books
No books were published during 2009.
1.2.6. Ph.D. theses
No Ph.D. theses were concluded during 2009.
1.2.7. MSc theses
1. Ana Catarina Dias Vieira da Silva
Evaluation of the levels of Hg, Cd, Pb and As in fish (sardine, horse mackerel and mackerel)
FFUP, February 2009.
2. José António Sousa
Benzene in Indoor Air and Exterior Air
FEUP, April 2009.
3. Helena Maria Vaz de Oliveira
Development of sensors for antibiotics
ISEP, July 2009.
[ 15 ]
4. Tânia Sofia Cardoso Ribeiro Rebelo
Implementation, validation and technical-economic analysis of some analytical parameters in the control of
water
ISEP, July 2009.
5. José Alberto Nunes da Silva Ferreira
Determination of pesticides in lettuce and irrigation water in the vulnerable zone of the free aquifer of
Esposende
FCUP, August 2009.
6. Marta Madalena Marques Oliveira
Evaluation of the mineral composition of soluble coffee and coffee substitutes
FFUP, September 2009.
7. Maria Isabel Mesquita Guimarães Seguro Pereira Soares
Development of methodologies for analysis of metals in natural waters by continuous-source atomic absorption
spectroscopy
FCUP, October 2009.
8. Felismina Teixeira Coelho Moreira
Determination of Norfloxacin in aquaculture
FCUP, November 2009.
9. Joana Rafaela Lara Guerreiro
Determination of chlortetracycline residues in aquaculture
FCUP, November 2009.
10. Joana Gomes Martins
Development of analytical methodologies for the determination of pesticides in wines of the Douro and Port
wines
ISEP, November 2009.
11. Fernando Odeberto Gonçalves Pereira
Voltammetric analysis of ciprofloxacin – application to pharmaceuticals and remediation
ISEP, December 2009.
12. Cleusa Lopes da Luz
Evaluation of Concentration of Minerals in Fish
13. Elisa Alexandra Mota Ferreira
Evaluation of different disinfection treatments of lettuce: A chemical and toxicological approach
FCNAUP, December 2009.
1.2.8.1. Oral presentations
1. Castro D., Slezakova K., Delerue-Matos C., Alvim-Ferraz M.C., Morais S., Pereira M.C.
Contribution of traffic and tobacco smoke in the distribution of Polycyclic aromatic hydrocarbons on outdoor and
indoor PM25
11th International Conference on Environmental Science and Technology CEST2009, 2009, Chania, Crete,
Greece.
2. Marta Maria Pereira da Silva Neves, Maria Begoña González-Garcia, Hendrikus Petrus Antonius Nouws, Maria
Alice dos Santos Silva Gomes Martins, Cristina Delerue-Matos, Agustín Costa-García
A novel transducer surface: screen-printed carbon electrodes modification with carbon-nanostructures and gold
nanoparticles
III Workshop Nanociencia y Nanotecnología Analíticas, 2009, Oviedo, Spain, Communication XXX.
3. L. Pinho, D. Castro, A. Begonha, S. Morais, M.C. Pereira, M.C. Delerue-Matos, M.C. Alvim-Ferraz,
Films Negros en Edifícios Históricos de Oporto: Caracterización y Experiencias de Limpieza
1º Congresso Iberoamericano e VIII Jornada de Técnicas de Restauração e Conservação do Patrimônio –
COIBRECOPA, 2009, La Plata, Argentina.
[ 16 ]
1.2.8.2. Poster presentations
1. Mónica Rosas, Teresa Oliva-Teles, Valentina Domingues, Cristina Delerue-Matos, António Crispim
Development of a methodology for evaluation of free-formaldehyde in leather
Fourth International Symposium on the Separation and Characterization of Natural and Synthetic
Macromolecules, P004, 2009, Amsterdam, Netherlands.
2. M.J. Ramalhosa, P. Paíga, S. Morais, M.B.P.P. Oliveira, C. Delerue-Matos
Polycyclic aromatic hydrocarbons in wild and cultured fishes
6th European Conference on Marine Natural Products PC35, 2009, Porto, Portugal.
3. Felismina T.C. Moreira, M.Goreti F. Sales
Biomimetric sensors for leucomalachite green
4. Ayman H. Kamel, Felismina T.C. Moreira, J. Rafaela L. Guerreiro, M. Goreti F. Sales
New enrofloxacin sensors for aquaculture environment
5. M.J. Ramalhosa, P. Paíga, S. Morais, M.B.P.P. Oliveira, C. Delerue-Matos
Analysis of priority polycyclic aromatic hydrocarbons in marine fish
EuroAnalysis 2009, P033-A1, 2009, Innsbruck, Austria.
6. A.C. Silva, S. Morais, C. Delerue-Matos, M.B.P.P. Oliveira
Heavy metal levels in muscle tissue of hurse mackerel (Trachurus Trachurus)
7. R.C. Alves, I.M.C. Almeida, S. Casal, C. Delerue-Matos, MBPP. Oliveira
Method development for daidzein, genistein and formononetin quantification in coffee
8. J.R.L. Guerreiro, M.G.F. Sales
Chlortetracycline-imprinted materials for its potentiometric determination in food and biological samples
9. F.T.C. Moreira, A.H. Kamel, M.G.F. Sales
Man-Tailored biomimetric sensor of molecularly-imprinted materials for the potentiometric measurement of
oxytetracycline
10. V.C. Fernandes, A. Dequeant, V.F. Domingues, N. Mateus, C. Delerue-Matos
Comparison of different QuEChERS for organochlorine pesticide determination in strawberries
11. M. Oliveira, S. Morais, S. Casal, C. Delerue-Matos, M.B.P.P. Oliveira
Application of high resolution continuum source flame atomic absorption spectrometry to sequential analysis of
Fe and Mn in instant coffee substitutes
12. Luz, A.C. Silva, C. Delerue-Matos, S. Morais, M.B.P.P. Oliveira
Macrominerals composition of sardine (Sardine Pilchardus) by microwave-assisted digestion and flame atomic
absorption spectrometry
13. T.J.R. Fernandes, E. Mendes, S. Casal, C. Delerue-Matos, M.B.P.P. Oliveira
Fat content and fatty acid composition of traditional dry cured sausages from Portugal
14. D. Castro, C. Delerue-Matos, M. C. Alvim-Ferraz, S. Morais, M. C. Pereira
Microwave-Assisted extraction of polycyclic aromatic hydrocarbons from polyurethane foam adsorbents
EuroAnalysis 2009, P054-B1, 2009, Innsbruck, Austria.
15. D. Castro, C. Delerue-Matos, M. C. Alvim-Ferraz, S. Morais, M. C. Pereira
Gas-Particle concentration and distribution of polycyclic aromatic hydrocarbons at an urban atmosphere
16. C. Pinho, P. Gameiro, C. Delerue-Matos, C. Pizarro, C. Coelho, A. Heitor, A. Tavares, C. Mansilha
Analysis of oils and greases in portuguese bathing waters. Implementation and validation of a FT-IR method
[ 17 ]
17. I. Seguro, J. Pinto, C. Mansilha, C. Delerue-Matos, M.T. Oliva-Teles, P. Gameiro
Analysis of metals in natural waters by high-Resolution continuum source atomic absorption spectrometry
18. C. Delerue-Matos, H.P.A. Nouws, M.M.P.S Neves
Carbon surfaces for the oxidative quantification of pravastatin: glassy-carbon vs. SPCE
19. D. Castro, L. Pinho, M.C. Alvim-Ferraz, C. Delerue-Matos, A. Begonha, S. Morais, M.C. Pereira
Characterization of the black cruts composition of an historic monument exposed to urban atmospheric pollution
20. A.M.M. Sousa, S. Morais, C. Delerue-Matos, Maria P. Gonçalves
Optimization of native agar from the red seaweed Gracilaria Vermiculophylla by microwave-assisted extraction
using response surface methodology
21. J.R.L. Guerreiro, A.H. Kamel, M.G.F. Sales
FIA potentiometric system based on periodate polymeric membrane sensors for the assessment of ascorbic
acid in commercial drinks
22. F.T.C. Moreira, G.G. Aguilar, M.G.F. Sales
New sulfadiazine-selective sensors of molecularly imprinted sol-gel material
23. M.F. Barroso, N. de-los-Santos-Álvarez, M.J. Lobo-Castanon, A.J. Miranda-Ordieres, M.G. Ferreira Sales,
M.B.P.P. Oliveira, C. Delerue-Matos
Investigations on the electrocatalytic assessment of antioxidant capacity using a DNA-Modified carbon paste
electrode
24. J.R.L. Guerreiro, M.G.F. Sales
Selective recognition in potentiometric transduction of amoxicillin by molecularly-imprinted materials
25. F.T.C. Moreira, G.G. Aguilar, M.G.F. Sales
Novel potentiometric sensors of ion-imprinted polymers for specific binding of iron and potentiometric
transduction
26. Dionisia Castro, Cristina Delerue-Matos, M.C. Alvim-Ferraz, Simone Morais, Maria C. Pereira
Impact of tobacco smoke on polyclic aromatic hydrocarbons levels in indoor PM2.5
ECNIS International Workshop biomarkers and Cancer, 2009, P7, 2009, Porto, Portugal.
27. V.C. Fernandes, V.F. Domingues, N. Mateus, C. Delerue-Matos
Organochlorine pesticides and biological evaluation in strawberries obtained from organic farming and
integrated pest management farming
New challenges in Food Preservation, P188, 2009, Budapeste, Hungria.
28. E. Ferreira, V.F. Domingues,C. Calhau, C. Delerue-Matos
Evaluation of different disinfecting treatments of lettuce: a chemical approach
New challenges in Food Preservation, P194, 2009, Budapeste, Hungria.
29. M. Fátima Barroso, J. Paulo Noronha, M. Goreti F. Sales, Cristina Delerue-Matos, M. Beatriz P.P. Oliveira
Development of a DNA-modified sensor to evaluate the total antioxidant capacity of flavoured waters
4th International Symposium on recent advances in food analysis, N-23, 2009, Prague, Czech Republic.
30. S. Duarte, J. Bento, A. Pena, C.M. Lino, J.A. Pereira, C. Delerue-Matos, T. Oliva-Teles, S. Morais, M. Correia,
M.B.P.P. Oliveira
Ochratoxin A contamination of bread-Portugal nationwide survey during the winter 2007/2008
4th International Symposium on recent advances in food analysis, G-7, 2009, Prague, Czech Republic.
31. Manuela Correia, Sandrine Eap, Artur Dias, Cristina Delerue-Matos
Head-space solid-phase microextraction for the determination of selected pesticides in water and apple samples
using gas chromatography-electron capture detection
4th International Symposium on recent advances in food analysis, E-1, 2009, Prague, Czech Republic.
[ 18 ]
32. S. Duarte, J. Bento, A. Pena, C.M. Lino, J.A. Pereira, C. Delerue-Matos, T. Oliva-Teles, S. Morais, M. Correia,
M.B.P.P. Oliveira, S. Casal
Ochratoxin A content in urine from Porto inhabitants: is there a relationship with season?
4th International Symposium on recent advances in food analysis, G-6, 2009, Prague, Czech Republic.
33. M.M.P. da S. Neves, M.B. González García, C. Delerue-Matos, A. Costa García
Superficies nanoestructuradas modificadas con estreptavidina como transductores de biosensores
XXX Reunión del Grupo de Electroquímica de la RSEQ y del XI Encontro Ibérico de Electroquímica. 2009.
Tenerife, Spain.
34. S.A.A. Almeida, A.M. Heitor, M.C.B.S.M. Montenegro, M.G.F.Sales
Selective sensors for sulfadiazine potentiometric transduction
rd
23 Eurosensors Conference, PX, 2009, Lausanne, Switzerland.
1.2.8.3. Proceedings papers
1. S.A.A. Almeida, A.M. Heitor, M.C.B.S.M. Montenegro, M.G.F.Sales
Selective sensors for sulfadiazine potentiometric transduction
Proceedings of the Eurosensors xxiii Conference, Procedia Chemistry 1 (1) 1031-1034 (2009).
2. D. Castro, K. Slezakova, C. Delerue-Matos, M. C. Alvim-Ferraz, S. Morais, M. C. Pereira
Contribution of Traffic and Tobacco Smoke in The Distribution of Polycyclic Aromatic Hydrocarbons on Outdoor
and Indoor PM2.5
Proceedings of the CEST2009, Chania, Crete, Greece, A131-137
3. L. Pinho, D. Castro, A. Begonha, S. Morais, M.C. Pereira, M.C. Delerue-Matos, M.C. Alvim-Ferraz
Films Negros en Edifícios Históricos de Oporto: Caracterización y Experiencias de Limpieza
Proceedings of the 1º Congresso Iberoamericano e VIII Jornada de Técnicas de Restauração e Conservação
do Patrimônio – COIBRECOPA, 2009, La Plata, Argentina, 1-13.
4.
No oral presentations in national conferences were made during 2009.
1. Almeida, R. Coelho, M.J. Ramalhosa, E. Mendes, P. Paíga, S. Casal, S. Morais, C. Delerue-Matos, M.B.P.P.
Oliveira
Characterization of the fatty acid profiles of sardine and chub mackerel
II Encontro de Jovens Investigadores da Universidade do Porto, 2009, Porto, Portugal, pp.220.
2. M. Oliveira, S. Morais, S. Casal, C. Delerue-Matos, M.B.P.P. Oliveira
Determination of Ca and Mg in instant coffee substitutes by high resolution continuum source
3. J. Ferreira, V. Domingues, N. Mateus, C. Delerue-Matos
Determination of pesticides in irrigation water using SPE and GC-ECD
4. C. Luz, A.C. Silva, A. Novo, E. Pinho, S. Conceição, S. Morais, C. Delerue-Matos, M.B.P.P. Oliveira
Arsenic analysis of chub mackerel muscle tissue
5. A.C. Silva, S. Morais, C. Delerue-Matos, M.B.P.P. Oliveira
Biometric characteristics of northeast Atlantic Ocean sardine (sardine pilchardus) and horse macherel
(Trachurus trachurus)
II Encontro de Jovens Investigadores da Universidade do Porto, 2009, Porto, Portugal, pp. 267.
6. P. Martins, D. Direito, N. Simões, P. Paíga, S. Morais, M. J. Ramalhosa, C. Delerue-Matos, M.B.P.P. Oliveira
Microwave-assisted extraction of fat fishes
7. J. Ferreira, A.M.M. Sousa, H. K. Souza, S. Morais, C. Delerue-Matos, M. P. Gonçalves
Rheological and UV properties of WPI/agar systems
[ 19 ]
8. Morais S., Direito D., Simões N., Martins P., Ramalhosa M.J., Paíga P., Delerue-Matos C., Oliveira M.B.P.P.
Comparison of several extraction procedures for lipid determination in fish
9º Encontro Quimica dos Alimentos P37, 2009, Angra do Heroismo, Açores, Portugal.
9. Barroso M.F., Ramos S., Delerue-Matos C., Sales M.F., Oliveira M.B.P.P.
Aguas naturais versus aromatizadas: Influência dos ingredientes adicionados na composição mineral
10. Silva A.C., Morais S., Delerue-Matos C., Oliveira M.B.P.P.
Determinação do teor de mercúrio e cádmio em cavala (Scomber japonicus)
11. Ramalhosa M.J., Paíga P., Morais S., Delerue-Matos C., Oliveira M.B.P.P
One step microwave-assisted alkaline digestion and extration of PAHs from fishes
12. C. Mansilha, A. Tavares, A. Melo, I. Ferreira, O. Pinho, V. Domingues, P. Gameiro
Detection and quantification of selected endocrine disrupters in drinking waters supplies in the north of Portugal
6º Encontro Nacional de Cromatografia, 2009, P-1, 2009, Madeira, Portugal.
13. Débora Soares, Manuela Correia, Cristina Delerue-Matos, M. Beatriz P.P. Oliveira
Analysis of selected pesticides in bovine milk by dispersive solid-phase extraction pollowed by gas
chromatography-electron capture detection
6º Encontro Nacional de Cromatografia, P-53, 2009, Madeira, Portugal.
1. Morais S., Direito D., Simões N., Martins P., Ramalhosa M.J., Paíga P., Delerue-Matos C., Oliveira M.B.P.P.
Comparison of several extraction procedures for lipid determination in fish
Proceedings of the 9º Encontro Quimica dos Alimentos, CD-ROM version, (2009).
2. Ramalhosa M.J., Paíga P., Morais S., Delerue-Matos C., Oliveira M.B.P.P
One step microwave-assisted alkaline digestion and extration of PAHs from fishes
3. Barroso M.F., Ramos S., Delerue-Matos C., Sales M.F., Oliveira M.B.P.P.
Aguas naturais versus aromatizadas: Influência dos ingredientes adicionados na composição mineral
4. Silva A.C., Morais S., Delerue-Matos C., Oliveira M.B.P.P.
Determinação do teor de mercúrio e cádmio em cavala (Scomber japonicus)
1.3. OTHER ACTIVITIES
Organization of conferences
On the 2nd and 3th of March of 2009 a seminar entitled "From Security to Food Quality" (“Da Segurança
à Qualidade do Alimento”) was organized at ISEP and had the following objectives:
i)
start a "culture of quality" in students, as potential employees of the food industry and just as
ordinary consumers;
ii)
confront the students with the idea of quality and strategies that could be adopted by different
industries and small food producers
iii)
contact with different ways to implement safety measures and guarantee quality of products.
The seminar was a large success, with around 400 participants.
[ 20 ]
Internationalization
Co-orientation with the University of Léon (Spain) of the PhD studies of Mónica Alexandra Oliveira Dias
Teixeira.
Co-orientation with the University of Oviedo (Spain) of the PhD studies of Marta Maria Pereira da Silva
Neves.
Collaboration with the University of Oviedo (Spain) and the University of Pernambuco (Brasil) of the PhD
studies of Maria de Fátima de Sá Barroso (FFUP).
Governmental funding (pre-graduation)
In 2008 and 2009 7 grants from the “Concurso de Bolsas de Integração na Investigação para 5000
estudantes do Ensino Superior” (FCT) were given to the following students:
1.
Adão Batista Martins Pinto, Sistema de apoio ao tratamento e gestão de resíduos laboratoriais.
2.
Ana Raquel dos Reis Ferreira, Bioremediação de solos.
3.
Laura Cristina Oliveira Sousa, Desenvolvimento de metodologia para a análise de ocratoxina A por
cromatografia líquida com detecção por espectrometria de massa.
4.
Liliana Adelina Afonso N. Almeida Truta, Sistemas Automáticos de Análise: Aplicação ao Método TRAP.
5.
Isa de Sousa Matos, Investigação de métodos electroquímicos para o doseamento de antidepressivos em
formulações farmacêuticas.
6.
Marcela de Jesus da Cunha Oliveira, em curso.
7.
Isabel Patrícia Ribeiro Moreira, em curso.
Launched in June 1996, the Ciência Viva programme is the contribution of the Ministry of Science and
Technology to the promotion of a scientific and technological culture among the Portuguese population. In
the sub-category “Science in the Holidays” 3 programs, with the duration of 1 week (40 hours) were
organized: “Análise Química de Produtos Farmacêuticos”, “Segurança à tua mesa”, “Crime Sob
Investigação no ISEP”.
In cooperation with a local professional high school (Escola Secundária Infante D. Henrique), 8 students
realized a professional training period of 160 hours:
1.
Carlos Manuel Teixeira Amorim, Análise de antibióticos por potenciometria - Tetraciclina.
2.
Cátia Maria Oliveira da Silva Carvalho, Determinação da gordura total em carapau recorrendo a vários
métodos.
3.
Cristiana Silva, Determinação de ocratoxina no pão.
4.
Joana Raquel da Silva Alves, Determinação de antibióticos por potenciometria - Clorpromazina.
5.
Patrícia Alexandra Santos Pacheco, Determinação do pesticida carbaril em fezes de rato e determinação de
pesticidas organoclorados em amostras de morangos.
6.
Rui Nuno Magalhães Brandão da Silva, Determinação de fósforo em cafés.
7.
Sara Eduarda da Costa Gonçalves, Determinação da gordura total em sardinha recorrendo a vários métodos.
8.
Vânia Alexandra Oliveira Marques, Determinação de nitratos e nitritos em vegetais.
[ 21 ]
1.
Andreia Patrícia Oliveira Toriz, Nitratos e nitritos em alfaces.
2.
Gustavo Vilaça Vale, Determinação de fósforo em peixe.
1.4. FUTURE RESEARCH
Approved funding (projects to be started in 2010):
PTDC/AGR-AAM/102316/2008, “Cephalopods: -Benefits and risks of consumption; -Evaluation of biomarkers
responses to organic pollution”, January 2010 - December 2012, € 80.296.
PTDC/AGR-AAM/102447/2008, “Spent coffee grounds: horticultural recovering program and implications in the
vegetables quality and safety”, January 2010 - December 2012, € 64.548.
Pending funding (projects submitted in 2009):
PTDC/AAC-AMB/112342/2009, “Endocrine disruptors and pesticides environmental pollution. An integrated risk
analysis approach”, € 134.090.
PTDC/AGR-ALI/111753/2009, “Organic farmed products versus others agricultural pratices. Strawberries as a case
study”, € 71.956.
PTDC/AGR-ALI/114303/2009, “TraceNutALL - Tracing tree nut allergens for improving the safety of food chain:
development of novel analytical methodologies and effect of processing on allergen detection”, € 186.768.
PTDC/AGR-ALI/114782/2009, “Nanobiosensor for rapid detection of brucella in milk and other dairy products”, €
199.188
PTDC/AGR-ALI/115070/2009, “SafeBio-Leite ao longo da vida humana: segurança e compostos bioactivos do
recém-nascido ao sénior”, € 123.332.
PTDC/MAR/113739/2009, “PPFISH – Aquatic species under psicopharmaceuticals neuroendocrine effects”, €
23.210.
PTDC/QUI-BIQ/115330/2009, “Development of a novel nanostructured electrochemically-based transducer surface:
application in the construction of immunosensors for the diagnosis of celiac disease and quality control of gluten-free
products”, € 124.157.
PTDC/SAU-ENB/114786/2009, “Nano-electrode arrays Biosensor for Early and Decentralized Breast-Cancer
Diagnosis”, € 171.270.
PTDC/ SAU-SAP/ 111921/ 2009, “Occupational Exposure and Environmental Impact of Polycyclic Aromatic
Hydrocarbons: Influence of Forest Fires”, € 67.012.
PTDC/AGR-ALI/114319/2009, “Propriedades antioxidantes dos Vinhos Verdes: autenticidade, caracterização e
intensificação”, € 47.234.
[ 22 ]
RESEARCH AREAS – ENVIRONMENTAL CONTROL AND REMEDIATION
2.
ENVIRONMENTAL CONTROL AND REMEDIATION
TEAM MEMBERS
Permanent Members
MSc
Bie van der Vliet
Maria Conceição Carvalho Benta de Oliveira Neves
Sónia Adriana Ribeiro da Cunha Figueiredo
Diana Margarida Ferreira Pinheiro
Florinda Figueiredo Martins
Olga Manuela Matos de Freitas
Joana Maria Cesar Veloso Ferreira
Maria Aurora Soares da Silva
Bruno José Rocha Pereira
Sérgio Alberto Cruz Monteiro de Morais
Maria Isabel Viana de Brito Limpo de Serra
Grant holders
António Carlos Alves Soares Pedro Romeu da Silva Soares
Ph.D.
Adão Batista Martins Pinto
Antonio Vega Y de la Fuente
Ana Raquel dos Reis Ferreira
Maria Manuela Martins de Carvalho
Irene Cristina de Sousa Azevedo
Virgínia Maria Monteiro Cruz Fernandes
FCT funded projects
1
non-FCT funded projects
2
Papers (ISI)
5
Papers (non-ISI)
--
Books
--
Ph.D. theses
--
MSc theses
10
Oral presentation
Poster presentation
Proceedings papers
Conferences (national)
Oral presentation
Poster presentation
Proceedings papers
Conferences (organization)
-17
3
-----
[ 23 ]
2.1. SUB-AREAS IN ENVIRONMENTAL CONTROL AND REMEDIATION
In the subsequent sections a summary of several achievements in environmental control and remediation
in 2009 are presented, for further reading the consultation of the published papers is recommended.
2.1.1. Waste management and toxicological evaluation
The treatment efficiency of laboratory wastewaters was evaluated and ecotoxicity tests with Chlorella
vulgaris were performed on them to assess the safety of their environmental discharge. For chemical
oxygen demand wastewaters, chromium (VI), mercury (II) and silver were efficiently removed by chemical
treatments. A reduction of ecotoxicity was achieved; nevertheless, an EC50 (effective concentration that
causes a 50% inhibition in the algae growth) of 1.5% (v/v) indicated still high level of ecotoxicity. For
chloride determination wastewaters, an efficient reduction of chromium and silver was achieved after
treatment. Regarding the reduction of ecotoxicity observed, EC50 increased from 0.059% to 0.5%, only a
0.02% concentration in the aquatic environment would guarantee no effects. Wastewaters containing
phenanthroline/iron (II) complex were treated by chemical oxidation. Treatment was satisfactory
concerning chemical parameters, although an increase in ecotoxicity was observed (EC50 reduced from
0.31% to 0.21%). The wastes from the kinetic study of persulphate and iodide reaction were treated with
sodium bisulphate until colour was removed. Although they did not reveal significant ecotoxicity, only over
1% of the untreated waste produced observable effects over algae. Therefore, ecotoxicity tests could be
considered a useful tool not only in laboratory effluents treatment, as shown, but also in hazardous
wastewaters management
2.1.2. Soil and groundwater remediation
The application of soil remediation technologies have been increasing in the last decades due the
identification and discovery of contaminated sites. In recent years, new technologies have been
developed and some well established technologies have been combined aiming the increase of the
remediation efficiency. The combination of soil vapor extraction (SVE) and bioremediation (BR) to
remediate soils contaminated with petroleum hydrocarbons is one successful example. SVE is the most
used remediation technology worldwide. It is an in-situ technology that is applicable to soils contaminated
with volatile or semi-volatile contaminants such as petroleum products and it uses vacuum pumping to
extract contaminated vapors from the unsaturated zone of the soil. BR is one of the most popular
remediation technologies due its low cost. It commonly uses native microorganisms of the soil to degrade
the contaminants but can also use inoculated microorganisms that degrade specific contaminants present
in soil, creating faster remediations. In the first stages of the remediation, SVE presents high extraction
rates that decrease significantly has the remediation time increases. At this point the SVE is stopped and
initiated the BR reducing the remediation cost and the energy consumption. Our team has been focused
on the evaluation of the impact on the remediation time and process efficiency of some soil properties
(contents of organic matter and water), operational parameters (airflow rate) and contaminant properties
(vapor pressure). It is also being developed an artificial neural network to the prediction of the remediation
time of a SVE process based only on some soil properties and the contaminant involved.
[ 24 ]
2.1.3. Removal of toxic compounds by means of adsorption strategies
One of the most widely used processes for the removal of low concentration pollutants, present in
industrial wastewaters, has been through adsorption with activated carbon as a final treatment. Due to the
high price of the activated carbon, research has recently been directed towards alternative sorbents.
Some of the alternative sorbents are native natural materials, like cork, peanut hulls and marine algae,
which are renewable resources and are available in huge quantities in Portugal. These materials are also
natural wastes originated from the cork industry, agriculture and the pharmaceutical/cosmetic industry,
respectively. Some of these of low-cost sorbents have been investigated as a replacement for current
costly methods of removing metals from solution. The sorption process using these materials are
becoming more accessible, making the process applicable even in small and medium scale industries.
A Box–Behnken factorial design coupled with surface response methodology was used to evaluate the
effects of temperature, pH and initial concentration in the Cu(II) sorption process onto the marine
macroalgae Ascophyllum nodosum. The effect of the operating variables on metal uptake capacity was
studied in a batch system and a mathematical model showing the influence of each variable and their
interactions was obtained. Study ranges were 10 – 40 ºC for temperature, 3.0 – 5.0 for pH and 50 –150
mg/L for initial Cu(II) concentration. Within these ranges, the biosorption capacity is slightly dependent on
temperature but markedly increases with pH and initial concentration of Cu(II). The uptake capacities
predicted by the model are in good agreement with the experimental values. Maximum biosorption
capacity of Cu(II) by A. nodosum is 70 mg/g and corresponds to the following values of those variables:
temperature = 40 ºC, pH= 5.0 and initial Cu(II) concentration = 150 mg/L. The use of peanut hulls, an
agricultural and food industry waste, for copper and lead removal through equilibrium and kinetic
parameters evaluation was studied. Equilibrium batch studies were performed in a batch adsorber. The
influence of initial pH was evaluated (3– 5) and it was selected between 4.0 and 4.5. The maximum
sorption capacities obtained for the Langmuir model were 0.21 ± 0.03 and 0.18 ± 0.02 mmol/g,
respectively for copper and lead. In bi-component systems, competitive sorption of copper and lead was
verified, the total amount adsorbed being around 0.21 mmol of metal per gram of material in both mono
and bi-component systems. In the kinetic studies equilibrium was reached after 200 min contact time
using a 400 rpm stirring rate, achieving 78 % and 58 % removal, in mono-component system, for copper
and lead respectively. Their removal follows a pseudo-second-order kinetics. These studies show that
most of the metals removal occurred in the first 20 min of contact, which shows a good uptake rate in all
systems. In another work we isolated from soil and characterized several bacterial strains capable of
either resisting high concentrations of heavy metals (Cd
2+
2+
or Hg
2+
or Pb ) or degrading the common soil
and groundwater pollutants MTBE (methyl-tertbutyl ether) or TCE (trichloroethylene). We then used soil
microcosms exposed to MTBE (50 mg/l) or TCE (50 mg/l) in the presence of one heavy metal (Cd 10
ppm or Hg 5 ppm or Pb 50 or 100 ppm) and two bacterial isolates at a time, a degrader plus a
metalresistant strain. Some of these two-membered consortia showed degradation efficiencies well
higher (49 – 182 % higher) than those expected under the conditions employed, demonstrating the
occurrence of a synergetic relationship between the strains used. Our results show the efficacy of the
dual augmentation strategy for MTBE and TCE bioremediation in the presence of heavy metals.
[ 25 ]
2.2. OUTPUT INDICATORS
Project reference:
Project title:
Duration:
Funding (total):
Funding (ICETA):
PTDC/ECM/68056/2006
Remediation of contaminated soils combining vapour extraction and biological
processes: time and efficiency forecasting
Cristina Maria Fernandes Delerue Alvim de Matos
Faculdade de Engenharia da Universidade do Porto
€ 52 555
€ 39 955
Project reference:
Project title:
Duration:
Funding source:
Funding (total):
Funding (ICETA):
Project reference:
Project title:
Duration:
Funding source:
Funding (total):
Funding (ICETA):
5551
FILMEQUE
April 2009 – March 2011
Antonio Alfredo Crispim Ribeiro
Curtumes Aveneda, Lda
Instituto Superior de Engenharia do Porto
Agência de Inovação (ADI)
€ 261 049
€ 78 137 (divided with CIETI-ISEP)
------Avaliação da eficiência de um coagulante/floculante de origem vegetal no
tratamento de águas e efluentes
November 2009 – October 2010
Rui Boaventura (FEUP)
ICETA/Instituto Superior de Engenharia do Porto
Universidade do Porto
€ 4 000
€ 2 000
2.2.3. Papers (ISI)
1.
F.D. Oliveira, J.H. Paula, O.M. Freitas, S.A. Figueiredo
Copper and lead removal by peanut hulls: Equilibrium and kinetic studies
Desalination 248 (1-3) (2009) 931–940.
2.
Olga Freitas, Cristina Delerue-Matos, Rui Boaventura
Optimization of Cu(II) biosorption onto Ascophyllum nodosum by factorial design methodology
Journal of Harzardous Materials 167 (1) (2009) 449-454.
3.
Aurora Silva, Sónia A. Figueiredo, M. Goreti Sales, Cristina Delerue-Matos
Ecotoxicity tests using the green algae Chlorella vulgaris—A useful tool in hazardous effluents management
Journal of Harzardous Materials 167 (1) (2009) 179-185.
4.
V.C. Fernandes, J.T. Albergaria, T. Oliva-Teles, C. Delerue-Matos, P. de Marco
Dual augmentation for aerobic bioremediation of MTBE and TCE pollution in heavy metal-contaminated soil
Biodegradation 20 (3) (2009) 375-382.
5.
G.A. Pinto, E.F. Gomes, F.O. Durão, C.M.N. Madureira, M.M.B.L. Guimarães, S. Morais
Multi-criteria design optimization study of solvent extraction in mixer-settler units
Hydrometallurgy 98 (3-4) (2009) 224-234.
[ 26 ]
No papers (non-ISI) were published during 2009.
2.2.5. Books
No books were published during 2009.
2.2.6. Ph.D. theses
No Ph.D. theses were concluded during 2009.
2.2.7. MSc theses
1.
Bie van der Vliet
Arsenic removal using natural and synthetic adsorbents
Karel de Grote-Hogeschool, Antwerpen, Belgium, June 2009.
2.
Global analyis on phosphorus: flow, supply horizon and impact assessment of phosphorus depletion
University of Southern Denmark, June 2009.
3.
Energy Optimization of a Furnace of a Ceramic Industry Sector
ISEP, July 2009.
4.
Avaliação ecotoxicológica de lixiviados de Lamas provenientes de estações de Tratamento de águas.
Capacidade de germinação de sementes de Alface (lactuca sativa)
5.
Diana Pinheiro
Oxidative leaching of metals from electronic waste with solutions based on quaternary ammonium salts
6.
Determination of the environmental impacts and life cycle analysis of biodiesel production
7.
Audit and Optimization for Energy Section of the impregnation of a textile industry
November 2009.
8.
Comparative study of changes in chemical characteristics of soil subjected to controlled burning and mechanical
thinning
9.
Simulation of the production of paraxylene
10. Joana Maria César Veloso Ferreira
Distribution of mercury in thermal waters and bottled in northern Portugal
No oral presentations in international conferences were made during 2009.
1.
Ana C. Meira Castro, J.T. Albergaria, João Paulo Meixedo, C.M. Delerue Matos, António Vivas, João Paulo
Carvalho, Eduarda Ferreira, Manuela Costa, Amélia Freitas
Soil Attributes Dynamics Evaluation After Prescribed Burning Practice in Northwestern Portugal Forest
FESP II - 2nd International Meeting of Fire Effects on Soil Properties, Marmaris, Turquia, 2009, pp. 56.
[ 27 ]
2.
Serra I, Morais S, Martins F.F, Delerue-Matos C.
TRELAB - Um modelo de gestão de resíduos químicos de laboratório como um contributo para a eco-eficiência
II Jornadas Luso-Brasileiras de Ensino e Tecnologia em Engenharia-JLBE 2009, pp.539, 2009, Porto, Portugal.
3.
Cruz J., Barros H., Guedes A., Morais S.
Optimização energética dos fornos de uma indústria do sector cerâmico
4.
Castro, A.C.M., Albergaria J.T., Meixedo J.P., Delerue-Matos C.M., Vivas A., Ferreira E., Costa M., Freitas A.,
Ribeiro S.R.
Ensaios preliminares para caracterização de um solo sujeito a fogo controlado
5.
Carvalho, G., Silva A., Delerue-Matos C., La Fuente A.V., Fiúza A.
Remediação do crómio usando ferro zerovalenete- Ensaios em coluna
6.
Oliveira F.D., Freitas A.M., Figueiredo S.A.
Optimização da biosorção de cobre, níquel e zinco utilizando o planeamento factorial
7.
A.M.M. Sousa, V.D. Alves, S. Morais, C. Delerue-Matos, Maria P. Gonçalves
Development and optimization of an ECO-Friendly agar extraction process from the red seaweed Gracilaria
Vermiculophylla
8.
Aurora Silva, Olga Freitas, Sónia Figueiredo, Bie Vandervliet, Adelaide Ferreira, António Fiúza
Arsenic removal using synthetic adsorvents: Kinetics, equilibrium and column study
12th EUCheMs International Conference on Chemistry and the Environment - ICCE2009, lno P35-231, 2009,
Stockholm, Sweden.
9.
Olga Freitas, Cristina Delerue-Matos, Rui Boaventura
Continuos-flow copper biosorption in regenerable Ascophyllum nodosum seaweed
12th EUCheMs International Conference on Chemistry and the Environment - ICCE2009, lno P12-208, 2009,
Stockholm, Sweden.
10. Oliveira F.D., Soares A.C., Freitas A.M., Figueiredo S.A.
Copper, nickel, and zinc removal by peanut hulls: batch and column studies in mono and tri-component systems
11th International Conference on -Environmental Science and Technology CEST2009, B36, 2009, Chania,
Crete, Greece.
11. Manuela Carvalho, António Fiúza, M. Teresa Oliva-Teles, Cristina Delerue-Matos, José Tomás Albergaria
Remediation of granitic soils contamined by MTBE using SVE- a case study
2nd international environmental best practices conference, P-63, 2009, Kraków, Poland.
12. Mónica Rosas, Valentina Domingues, Paolo Marco, Teresa Oliva-Teles, José Tomás Albergaria, Cristina
Delerue-Matos
Remediation of humic soils contamined with benzene using bioremediation
13. José Tomás Albergaria, Maria Conceição Alvim-Ferraz, Cristina Delerue-Matos
Remediation of soils contamined with trichloroethylene
14. Guedes, N. Ribeiro, J. Rocha, S. Morais
Auditoria energética à secção de impregnação de uma industria têxtil
3rd international congress on energy and environment engineering and management, 2009, pp. 19, Portalegre,
Portugal.
15. S. Teixeira, C. Delerue-Matos, L. Santos
The role of pH on sulfamethoxazole removal by walnut shells
INNOVA-MED conference, pp. 119, 2009, Girona, Spain.
16. S. Machado, M.T. Oliva-Teles, C. Mansilha, C. Delerue-Matos, P. Gameiro
Waste management and treatment recovery of acetronitrile
17. J. Ferreira, I. Seguro, T. Oliva-Teles, C.M. Matos, A. Veja
Distribuição do mercúrio nas águas hidrotermais do norte de Portugal
Encontro Internacional de termalismo e Turismo Termal/III Fórum Ibérico de águas engarrafadas e Termalismo,
Furnas, Açores, Portugal, 2 a 9 Novembro 2009.
[ 28 ]
1.
F. D. Oliveira, O. M. Freitas, S. A. Figueiredo
Copper, nickel and zinc removal by peanut hulls: batch and column studies in mono and tri-component systems,
Proceedings of the 11th International Conference on -Environmental Science and Technology CEST2009,
B676-B683 (2009).
2. Cruz J., Barros H., Guedes A., Morais S.
Optimização energética dos fornos de uma indústria do sector cerâmico
Proceedings of the II Jornadas Luso-Brasileiras de Ensino e Tecnologia em Engenharia - JLBE 2009, 205-211
(2009).
3. Serra I, Morais S, Martins F.F, Delerue-Matos C.
TRELAB - Um modelo de gestão de resíduos químicos de laboratório como um contributo para a eco-eficiência
Proceedings of the II Jornadas Luso-Brasileiras de Ensino e Tecnologia em Engenharia - JLBE 2009, 539-545
(2009).
No oral presentations in national conferences were made during 2009.
No poster presentations in national conferences were made during 2009.
No poster presentations in national conferences were made during 2009.
2.3. OTHER ACTIVITIES
Governmental funding (pre-graduation)
In 2008 and 2009 2 grants from the “Concurso de Bolsas de Integração na Investigação para 5000
estudantes do Ensino Superior” (FCT) were given to the following students:
1.
Pedro Romeu da Silva Soares, Adsorção de micropoluentes (metais) usando materiais naturais e sintéticos.
2.
Irene Cristina de Sousa Azevedo, em curso.
Launched in June 1996, the Ciência Viva programme is the contribution of the Ministry of Science and
Technology to the promotion of a scientific and technological culture among the Portuguese population. In
the sub-category “Science in the Holidays” 3 programs, with the duration of 1 week (40 hours) were
organized: “Os Engenheiros Incendiários”, “Valorização de resíduos naturais”, “Vamos procurar
processos mais sustentáveis usando a Química Verde!”
1.
Ana Cláudia Santos Silva, Pontos negros de contaminação e análise de solos.
2.
Ana Isabel Ribeiro Meireles, Gestão de laboratório.
3.
Cristiano Jorge Madeira Azevedo, Recuperação de solventes orgânicos.
4.
Ivo Emanuel Moreira Rodrigues, Gestão e tratamento de resíduos laboratoriais.
5.
Liliana Andreia Nogueira Oliveira, Gestão de laboratório.
[ 29 ]
6.
Lisandra Bastos, Bioremediação de solos.
7.
Rui Manuel Soares de Sousa, Tratamento e gestão de resíduos laboratoriais provenientes dos laboratórios do
ISEP.
8.
Telma Magalhães, Pontos negros de contaminação e análise de solos.
2.4. FUTURE RESEARCH
Approved funding in 2009 (projects to be started in 2010):
PTDC/ECM/103141/2008, “Rehabilitation of pharmaceuticals-contaminated soils”, January 2010 - December 2012, €
98.764.
Pending funding (projects submitted in 2009):
PTDC/AAC-AMB/115154/2009, “Valorização de penas (resíduo da indústria avícola) para tratamento de águas e
efluentes“, € 86.653.
PTDC/AAC-AMB/112638/2009, “Sustainable management of laboratory chemicals and wastes”, € 54.993.
PTDC/AAC-AMB/113386/2009, “Sustainable chemical laboratories - energy optimization using standard domotic
infrastructures in indoor environment quality control”, € 80.730.
PTDC/ECM/114112/2009, “Cinética da Bioventilação – uma abordagem multidisciplinar”, € 15.320.
[ 30 ]
RESEARCH AREAS – RESEARCH METRICS
3.
RESEARCH METRICS
TEAM MEMBERS
Permanent Members
--
Oral presentation
Poster presentation
Proceedings papers
-1
1
The development of metrics to assess research outputs is recognized of increased importance worldwide.
For many years, ISI (Thomson Scientific, Inc.) has provided the sole standardized set of bibliometric data
that were used in all sort of studies. More recently, Elsevier has been developing an alternative database,
Scopus, which promises to be a useful competitor and provide a different point of view over scientific
publication. Other measures have been used in different countries, the case of the UK deserving
emphasis as the plan to abandon the costly periodic Research Assessment Exercises elicited heated
discussion and many serious studies.
It is common that university groups develop studies that are then used by government agencies for their
official studies and benchmarks of research performance. In Portugal no systematic work exists and
government publications (of GPEARI under the ministry of Science and Higher Education) depend
directly on data provided by Thomson Scientific. Requimte is developing bibliometric studies of
Portuguese research institutions and intends to develop other type of metrics to assess the outcomes of
scientific research.
3.1.
OUTPUT INDICATORS
1. Elizabeth S. Vieira, Henri P.A. Nouws, José T. Albergaria, Cristina Delerue Matos, José A.N.F.
Gomes
Research Quality Indicators for Brazilian, Portuguese and Spanish Universities
th
12 International Conference on Scientometrics and Informetrics – ISSI2009, P50, Rio de Janeiro,
Brasil.
1. Vieira E.S., Nouws H.P.A., Albergaria J.T., Delerue Matos C., Gomes J.A.N.F.
Research Quality Indicators for Brazilian, Portuguese and Spanish Universities
th
Proceedings of the ISSI2009 - 12 International Conference on Scientometrics and Informetrics,
vol 2, 1000-1001 (2009).
[ 31 ]
FUNDING AND SCIENTIFIC EVALUATION
B.
SCIENTIFIC EVALUATION
Although there is no clear trend, the number of papers published in scientific journals included in the ISI
Web of Science database in 2009 was by far the highest for GRAQ in the period 2001-2009 (Figure 5.1).
However, the observed annual increase from 2007 is believed to continue in 2010, because already 12
papers have been published as of March 2010.
20
Papers ISI Web of Science
18
16
14
12
10
8
6
4
2
0
2001
2002
2003
2004
2005
2006
2007
2008
2009
Year
Figure 5.1 Number of papers published in scientific journals included in the ISI Web of Science by
GRAQ members (2001-2009)
The number of papers published per Ph.D. member of the permanent staff (Figure 5.2) varies between
0.3 and 1.5. The same tendency is observed for the number of papers: steady increase from 2007.
The results from a citation analysis for the period 2001-2009 are shown in Figure 5.3. An obvious
decrease in citations per paper is observed because of (i) the low number of papers and (ii) the short time
period. The average citation per paper is 3.4 and the Hirsch index (h-index) is 8.
[ 32 ]
FINANCIAL AND SCIENTIFIC EVALUATION
1,6
Papers / PhD-staff
1,4
1,2
1,0
0,8
0,6
0,4
0,2
0,0
2001
2002
2003
2004
2005
2006
2007
2008
2009
Year
Figure 5.2 Number of papers published in scientific journals included in the ISI Web of Science per
Ph.D. member of the permanent staff (2001-2009)
10,0
9,0
Citations / paper
8,0
7,0
6,0
5,0
4,0
3,0
2,0
1,0
0,0
2001
2002
2003
2004
2005
2006
2007
2008
2009
Year
Figure 5.3 Citation analysis (2001-2009)
[ 33 ]

2009 - GRAQ

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