Fig. S1. Maximum likelihood tree of the green algae inferred from

Fig. S1. Maximum likelihood tree of the green algae inferred from

0.1 substitutions per site
Chlorophyceae
93/100
Lake Fryxell VII2 (McM; FJ946904)
55/85
Lake Fryxell VI12 (McM; FJ946905)
Lake Ace II4 (VH, McM; FJ946902)
97/100 89/89
cf. Chlorococcum sp. 105-99 (Ar, Ter; AF514408)
Chloromonas perforata (NT, FW; U70794)
Pleurastrum insigne (NT, Ter/L; Z28972)
73/- Chlorococcum oleofaciens (NT, Ter; U41176)
100/100
Chlamydopodium vacuolatum (Tr, Ter; M63001)
chlamydomonad sp. Pic 8/18 P-17w (NT, FW; AY220091)
75/99
55/89
VPL9-6 (VP; FJ946907)
Ettlia minuta (Tr, Ter; M62996)
51/85/100
66/Ascochloris multinucleata (NT, Ter, U63106)
Spongiochloris spongiosa (NT, FW; U63107)
87/100
Chlorosarcinopsis minor (NT, FW; AB049415)
58/85
Haematococcus zimbabwiensis (Tr, Ter; U70797)
Chlamydomonas sp. (Palmer station, An, Mar; EF106784)
98/100
66/98
Palmellopsis sp. BCP-EM1VF1 (NT, Des; AY271673)
Chlorogonium euchlorum (ST, Ter; AJ410443)
81/100
Haematococcus pluvialis (NT, FW; AF159369)
73/99
Dunaliella salina (Ar, Saltlake; EF473743)
62/100
Chlamydomonas subcaudata (NT, FW; AJ781310)
Chlamydomonas applanata (NT, Ter; U13984)
98/100
Characiosiphon rivularis (NT, Ter; AF395437)
Chlamydomonas pseudopulsatilla (Ar, Ter; AF514405)
-/100
-/92
Microglena monadina (NT, FW; AY220559)
88/100
Chlamydomonas pulsatilla (Ar, Ter; AF514404)
Pseudotetracystis sp. NB1-VF11 (NT, Des; AY614714)
Chlamydomonas chlamydogama (NT FW; AY220560)
Chlamydomonas noctigama (NT, Ter; AF008241)
-/94
Chlamydomonas hedleyi (NT, FW; AJ781312)
100/100
uncultured chlorophyte (NT, Mar;AY180047)
100/100
-/96
Chlamydomonas sp. SAG 75.94 (Ar, Ter; AF514399)
100/100
100/100 Chlamydomonas raudensis (Lake Bonney, An, FW; AJ781313)
92/100
Chloromonas nivalis (Ar, Ter; AF514409)
100/100
Chloromonas cf. platystigma (Ar, Ter; AF514401)
76/100
Chloromonas reticulata (NT, FW; AJ410448)
Actinochloris sp. BCP-LG3VF20 (NT, Des; AY271674)
97/100
Chlorococcum cf. tatrense (Ar, Ter; AF514407)
87/100
uncultured chlorophyte (NT, Ter; EF023280)
uncultured chlorophyte (NT, Ter; EF024027)
100/100 99/100
Chlamydocapsa sp. MX219-VF21 (NT, Des; AY614713)
-/95
chlorococcalean sp. 102/98 (Ar, Ter; AF514411)
-/71 VPL9-5 (VP; FJ946901)
chlamydomonad sp. Pic8/18T-9d (NT, FW; AY220589)
100/100 100/100
Asterococcus superbus (NT, FW; AB175836)
71/Asterococcus korschikoffii (NT, FW; AB175837)
Lobochlamys segnis (NT, FW; AJ410464)
-/87
Gloeococcus maximus (Battleship Promontory, An, Ter; U83122)
-/100
56/100
Chlamydomonas baca (NT, FW; U70781)
100/100
Tetracystis sp. BCP-ZNP3-36 (NT, Des; AY377439)
Chlamydomonas reinhardtii (NT, FW; M32703)
100/100
86/99
Paulschulzia pseudovolvox (NT/Ar, Ter; U83120)
Tetraspora sp. (NT, FW; U83121)
Spermatozopsis similis (NT, FW; X65557)
Carteria obtusa (NT, Ter; AF182818)
Sphaeroplea robusta (NT, FW; U73472)
Trochiscia hystrix (ST, FW; AF277651)
100/100
Cylindrocapsa geminella (NT, FW; U73471)
Golenkinia longispicula (NT, FW; AF499923)
Schizomeris leibleinii (NT, FW; AF182820)
90/100
Uronema belkae (NT, FW; AF182821)
100/100
65/93
Chaetophora incrassata (NT, FW; D86499)
Dictyochloris fragrans (NT, Ter;AF367861)
Chaetopeltis orbicularis (NT, FW; U83125)
100/100
Radiococcus polycoccus (NT, FW; AF388378)
63/85
Lake Ace I5 (VH; FJ946892)
Pseudomuriella aurantiaca (NT, FW; AB005748)
Planktosphaeria gelatinosa (NT, FW; AY044648)
Bracteacoccus giganteus (NT, Ter; U63099)
Pseudoschroederia antillarum (NT, FW; AF277649)
100/100 VPL4-4 (VP; FJ946900)
chlorophycean sp. Tow 2/24 P-12d (NT, FW; AY197633)
Kirchneriella aperta (NT, FW; AJ271859)
Pseudodictyosphaerium jurisii (NT, FW; AF106074)
68/100
Pediastrum duplex (NT, FW; M62997)
Characiopodium hindakii (Tr, Ter; M63000)
Bracteacoccus sp. ZNP1-VF32 (NT, Des; AF513379)
53/86
Scenedesmaceae sp. Tow 9/21 P-14w (NT, FW; AY197639)
100/100
71/100
Desmodesmus subspicatus (NT, FW; AJ249514)
Neodesmus pupukensis (ST, FW; X91267)
Scenedesmus sp. SEV3VF49 (NT, Des; AF513373)
96/100
Scenedesmus obliquus (NT, FW; X56103)
Scenedesmus deserticola (NT, Des; AY510462)
94/100
Coelastrum sphaericum (NT, FW; AF388376)
Scenedesmus
pectinatus var. pectinatus (NT, FW; AB037092)
70/97
52/- Scenedesmus regularis (NT, FW; AB037095)
Scenedesmus sp. Antarctic (Gondwana Lake, An, FW; AM419228)
52/99
Lake Ace II11 (VH, R, WO, EO, VP, Beak, SK, LA; FJ946893)
Scenedesmus arcuatus (NT, FW; AY170311)
80/98
Scenedesmus obtusus (Ar/NT, FW; AB037091)
Coelastrella saipanensis (AB055800)
94/100
Coelastrella terrestris (Mt. Erebus, An, Ter; AB012847)
95/100 Scenedesmus sp. CCMP 1625 (Mt. Erebus, An, Ter; AF339735)
100/100
Oedogonium cardiacum (NT, FW; U83133)
Bulbochaete hiloensis (NT, FW; U83132)
Oltmannsiellopsis viridis (NT, FW; D86495)
50/94
Trichosarcina mucosa (Vestfold Hills, An, FW; AM109906)
66/100
86/100
Monostroma grevillei (NT, Mar; AF015279)
Pseudendoclonium akinetum (NT, FW; DQ011230)
100/100 Hazenia mirabilis (NT, FW; AF387156)
100/100
Ulva intestinalis (NT, Mar; AJ000040)
100/100
Blidingia dawsonii (NT, Mar; DQ001138)
Acrosiphonia arcta (NT, Mar;AY303600)
71/94
Protomonostroma undulatum (NT, Mar; DQ821517)
100/100
uncultured Urospora (Terra Nova Bay, An, Mar; AJ626846)
99/100
Chlorothrix sp. ChloPac47SI (NT, Mar;AY476827)
100/100
100/100 Lake Fryxell VI11 (McM, WO, EO, VP; FJ946884)
56/- Chlorella sp. NJ-18 (An, Ter; DQ377324)
Chlorella sp. (NT, FW; AY197624)
Lake Fryxell VI2 (McM; FJ946883)
100/100
Chlorella pyrenoidosa (NT, FW; AB240151)
60/93
Chlorella sp. YEL (NT, Ter; DQ377322)
72/100
Chlorella lobophora (NT, Ter; X63504)
89/89
Chlorella vulgaris (NT, FW; X13688)
Chlorella vulgaris NJ-7 (An, Ter; DQ377323)
Dictyosphaerium pulchellum (NT, FW; AY323838)
58/54/- Chlorella sp. FACHB31 (Tr, Ter; DQ377321)
63/70/- Chlorella sorokiniana (NT, Ter; X73993)
Muriella terrestris (NT, Ter; AB012845)
Chlorella minutissima (NT, Ter; X56102)
VPL1-3 (VP; FJ946890)
-/85
Nannochloris sp. ANR-9 (NT, FW; AY220081)
Koliella spiculiformis (NT, FW; AF278744)
88/100
51/100
Marvania geminata (NT, FW/Ter; AF124336)
Picochlorum eukaryotum (NT, Mar; X06425)
Nannochloris sp. Ant-1 (An, Ter; EF440182)
100/100
Nannochloris sp. JL 4-6 (NT, FW; AY195983)
70/99
Prototheca wickerhamii (NT, Ter; X74003)
Radiofilum conjunctivum (NT, FW; AF387155)
Eremosphaera viridis (Tr, FW; AF387154)
100/100
Oocystis solitaria (NT, FW; AF228686)
86/100
100/100
Oocystis heteromucosa (Tr, FW; AF228689)
Amphikrikos cf. nanus (NT, FW; AF228690)
100/100 Stichococcus sp. NB2VFF10 (NT, Des; AF513370)
86/Stichococcus chodati (NT, Ter; AB055867)
62/94
Stichococcus sp. FACHB753 (Tr, EU045358)
Prasiola stipitata (NT, Mar; EF200524)
83/100
-/99
Stichococcus bacillaris (NT, Ter; AB055866)
50/EO7-4 (EO; FJ946882)
59/Koliella longiseta (NT, Ter/FW; U18520)
100/100
54/Raphidonema brevirostre (Ar, Ter; AF514410)
Chlorella sp. (Ar, Ter; AF514413)
100/100
Pabia signiensis (Signy Island, An, Ter; AJ416108)
100/100
Elliptochloris subsphaerica (Chapman Ridge, Ant, Ter; AB006050)
75/100
73/92
Chlorella ellipsoidea (NT, FW; X63520)
Chlorella mirabilis (NT, Ter; X74000)
Coenocystis inconstans (NT, Ter; AB017435)
Parietochloris pseudoalveolaris (NT, Ter; M63002)
Coleochlamys perforata (NT, Ter/Snow; M62999)
Leptosira terrestris (NT, Ter; Z28973)
84/100
uncultured Trebouxia sp. (McMurdo dry valleys, An, Ter; AY250848)
Trebouxia asymmetrica (NT, Ter/L; Z21553)
93/100
100/100
Trebouxia usneae (NT, Ter; Z68702)
Friedmannia israeliensis (NT, Ter; M62995)
Trebouxia magna (NT, Ter/L; Z21552)
99/100
SC2-2 (SC; FJ946881)
Watanabea reniformis (NT, FW; X73991)
77/97
Dictyochloropsis reticulata (NT, Ter; Z47207)
Lobosphaera tirolensis (NT, Ter; AB006051)
Choricystis sp. (NT, FW; AY195970)
78/100
Choricystis
minor
(NT/Ar, FW; X89012)
99/100
Botryococcus sp. (NT, FW; AY197640)
Coccomyxa glaronensis (NT, Ter/L; AM167525)
81/100
100/100
Coccomyxa pringsheimii (NT, Ter/L;AY762603)
Paradoxia multiseta (NT, FW; AY422078)
93/100
Paradoxia sp. 294-GA206 (Ar, Ter; EU282454)
60/97
59/94 VPL5-6 (VP, EO; FJ946891)
Scherffelia dubia (NT, FW; X68484)
uncultured prasinophyte (Ar, Mar; EF100340)
100/100
100/100
Tetraselmis striata (NT, Mar; X70802)
Picocystis salinarum (NT, Saltern ;AF125167)
Pseudoscourfieldia marina (NT, Mar; X75565)
Nephroselmis olivacea (NT, FW; X74754)
Pyramimonas disomata (Tr, Mar; AB017121)
Pyramimonas australis (Terra Nova Bay, An, Mar; AJ404886)
Pterosperma cristatum (NT, Mar; AJ010407)
Cymbomonas tetramitiformis (NT, Mar; AB017126)
Pycnococcus provasolii (NT, Mar; X91264)
Dolichomastix tenuilepis (NT, Mar; AF509625)
Crustomastix stigmatica (NT, Mar; AJ629844)
Ostreococcus tauri (NT, Mar;AY329635)
100/100
Mamiella sp. (NT, Mar; AB017129)
Mantoniella squamata (NT, Mar; X73999)
100/100
84/91
Mantoniella antarctica (Lützow Holmbukta, An, Mar; AB017128)
100/100 Micromonas pusilla (An, Mar; DQ025753)
Micromonas pusilla (NT, Mar;AY954999)
Mesostigma viride (NT, FW; AJ250109)
Chaetosphaeridium globosum (NT, FW; AF113506)
Chlorokybus atmophyticus (NT, FW; AF408244)
Streptophyta
Cylindrocystis brebissonii (NT, FW; AF115439)
Ancylonema nordenskioeldii (Ar, Ter; AF514397)
Fig. S1. Maximum likelihood tree of the green algae
inferred from 18S rDNA sequences. Numbers at
nodes indicate statistical support: ML bootstrap
values (> 50) and BI posterior probabilities (> 90, in
percent) are indicated at branches. Phylogenetic
positions of freshwater and terrestrial Antarctic
sequences are indicated in red, Arctic sequences in
blue. Strains obtained during this study are in bold
and indicated by strain name (see Tables 1 and 2). In
addition to species names, climatic region (NT; north
temperate, ST; south temperate, Tr; tropical, Ar;
Arctic, An; Antarctic), environment (FW; freshwater,
Mar; marine, Ter; terrestrial) and GenBank accession
numbers are given within parentheses. Regions from
which identical sequences were retrieved are
indicated in parentheses (McM; McMurdo Dry
Valleys, VH; Vestfold Hills, VP; View Point, R; Rauer
Islands, WO; West Ongul, EO; East Ongul, Beak;
Beak Island, SK; Skarvsness, LA; Langhovde, SC;
Schirmacher Oasis).
Ulvophyceae
Trebouxiophyceae
74/87
76/99
86/100
81/89
100/100
63/99/100
98/100
60/-
100/100
Prasinophyceae
96/
100