Dr. Hasso Seibert Institut für Toxikologie und Pharmakologie für

Dr. Hasso Seibert
Institut für Toxikologie und Pharmakologie für Naturwissenschaftler
Universitätsklinikum S-H
Brunswiker Str. 10
D-24105 Kiel
GERMANY
BIBLIOGRAPHY
SEIBERT, H. (1978) Der Einfluß des Nervus vagus auf die Herzfrequenz bei der
Temperaturadaption des Aales (Anguilla anguilla L.) Verh. Dtsch. Zool. Ges., 201
JANKOWSKY, H.-D. & SEIBERT, H. (1978) Die Wirkung der Temperatur auf die
Glykogenolyse isolierter Leberzellen von Fischen. Verh. Dtsch. Zool. Ges., 208
SEIBERT, H. (1979) Thermal adaptation of heart rate and its parasympathethic control in the
european eel Anguilla anguilla L. Comp. Biochem. Physiol. 64 C, 275-278
SEIBERT, H. (1980) Effect of temperature on glycogen metabolism in isolated liver cells of
rainbow trout. European Soc. of Comp. Biochem. Physiol., Southhampton, England
HOTOPP, W., JANKOWSKY, H-D. & SEIBERT, H. (1980) Criteria of viability - succinate
stimulation of respiration and adenine nucleotide content - in isolated liver cells of rainbow trout.
Europ. Soc. of Comp. Biochem. Physiol., Southhampton, England
JANKOWSKY, H-D. & SEIBERT, H. (1981) Effect of temperature on carbohydrate metabolism
in liver cells of rainbow trout. Soc. Exp. Biol., Durham, England
JANKOWSKY, H-D., HOTOPP, W. & SEIBERT, H. (1984)
Influence of thermal acclimation on glucose production and ketogenesis in isolated eel hepatocytes,
Am. J. Physiol. 247, R 471-R 478
SEIBERT, H. (1985) Viability control and oxygen consumption of isolated hepatocytes from
thermally acclimated rainbow trout (Salmo gairdnerii). Comp. Biochem. Physiol. 80 B, 677-683
SEIBERT, H. (1985) Effects of temperature on glucose release and glycogen metabolism in
isolated hepatocytes from rainbow trout. Comp. Biochem. Physiol. 81 B, 877-883
SEIBERT, H., KOLOSSA, M. & WRANGEL, R. (1986) Mammalian spermatozoa as in vitro
model for studies on acute cytotoxicity of chemicals. Naunyn-Schmiedeberg's Arch. Pharmacol.
332, R 19
SEIBERT, H. & KOLOSSA, M. (1987) Quantitative structure-toxicity relationships of substituted
phenols determined in vitro with a sperm motility assay.
in: Alternatives to animal experiments in risk assessment, Proceedings, 135
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SEIBERT, H. (1987) Messung der Bewegungsaktivität von Bullenspermien mit Hilfe von
Videomikrographie und Computer Bildanalyse I und II.
35. Internationale Fachtagung für Fortpflanzung und Besamung, Wels, Österreich.
SEIBERT, H. (1988) Messung der Bewegungsaktivität der Spermatozoen von Mensch und Rind
mit Hilfe von Videomikrographie und Computerbildanalyse. Fertilität 4, 215-218
WRANGEL, R. & SEIBERT, H. (1988) Anwendung der Computer - Videomikrographie zur
Charakerisierung menschlicher Ejakulate. Physiologie und Pathologie der Fortpflanzung,
Hannover; Zuchthyg. 23, 102
SEIBERT, H., GOSCH, U., REIMER, M. & VIEHMANN, K. (1988) Analysis of the cytotoxic
action of chemicals by means of bovine spermatozoa in vitro. Naunyn-Schmiedeberg's Arch.
Pharmacol. 337, R 24
KOLOSSA, M., MÖLLER, G. & SEIBERT, H. (1989) Objektive Beurteilung der
Bewegungsaktivität von Rindertiefgefriersperma mit Hilfe von Videomikrographie und Computer Bildanalyse. Dtsch. Tierärztl. Wschr. 96, 266-269
SEIBERT, H., KOLOSSA, M. WASSERMANN, O. (1989) Bovine spermatozoa as an in vitro
model for studies on the cytotoxicity of chemicals: effects of chlorophenols. Cell Biol. Toxicol. 5,
315-330
DE BEER, C., ALSEN-HINRICHS, C., KRUSE, H., NOACK, G. & SEIBERT, H. (1989)
Bestimmung von Blei, Cadmium, Selen und Zink in Humansperma. CAS, 5. Colloqu.
Atomspektrom. Spurenanal. (WELZ, B., Ed.), 887-897
ARMBRUST, B. & SEIBERT, H. (1989) Der Einfluß umweltrelevanter Schwermetalle auf die
Spermatogenese in vivo und auf Spermatozoen in vitro - eine Literaturübersicht - Schriftenr. Abt.
Toxikol., Univ. Kiel; 15, 1-54
ASCHMANN, C., GÜLDEN, M., SEIBERT, H. & WASSERMANN (1989) Anwendung
tierischer Zellkulturen als Ergänzung zum Tierversuch. Abschlußbericht zum BMFT-Projekt
0318784A - Teilprojekt 1
NOACK, G., DE BEER, C. & SEIBERT, H. (1990) Blei Cadmium, Selen and Zink im Sperma
beruflich nicht exponierter Männer. Fortschritte der Fertilitätsforschung 18, 248
HANSEN, I, KRUSE, H., & SEIBERT, H. (1990) Persistente Organochlorverbindungen im
menschlichem Blut und Sperma. Fortschritte der Fertilitätsforschung 18, 250
SEIBERT, H. & GOSCH, U. (1990) A short-term bovine sperm cell assay for the evaluation of the
in vitro cytotoxicity of chemicals. ATLA 17, 228-232
SEIBERT, H. (1990) Alternativmethoden in der Toxikologie. - Sind Tierversuche ersetzbar?
Therapiewoche 40, 927-942
PAGE 11
KOLOSSA, M. & SEIBERT, H. (1990) A chemically "defined" diluent for cryopreservation of
bovine spermatozoa. Andrologia 22, 445-454
VOSS, J.-U., MÖRCHEL, S., SEIBERT, H. (1990) Primary cultures of rat hepatocytes on
microcarriers as a xenobiotic metabolising system in cocultures. VIIIth. Scand. Workshop on In
Vitro Toxicol., Kongsvoll, Norwegen
WIENCKE, T. & SEIBERT, H. (1990) Organische Umweltchemikalien und männliche Fertilität.
Schriftenr. Abt. Toxikol., Univ. Kiel; 17: 1-73
VOSS, J.-U. & SEIBERT, H. (1991) Metabolism mediated cytotoxicity detected by cocultures of
microcarrier-attached rat hepatocytes and BALB/c 3T3 cells. Naunyn-Schmiedeberg's Arch.
Pharmacol. 343 (Suppl.) R17
KOLOSSA, M. & SEIBERT, H. (1991) Toxicity testing by means of cryopreserved bovine
spermatozoa. ATLA 19, 204-208
EKWALL, B., BARILE, F.A., BJERREGARD, H., CLOTHIER, R.H., DIERICKX, P.J.,
FISKESJÖ, G., GARZA-OCANAS, L., GOMEZ-LECHON, M.J., HELLBERG, S., NORDIN,
M., SEIBERT, H., SHRIVASTAVA, R., TINGLEFFSKAANILD, M., TYSON, C., VALONE,
S., ZUCCO, F., WALUM, E. (1991) Prediction of acute human toxicity for the first 10 MEIC
chemicals by altogether 50 cytotoxicity assays. World Congress on Cell and Tissue Culture,
Anaheim, CA, USA
KOLOSSA, M. & SEIBERT, H. (1991) Cryopreservation of mammalian spermatozoa by means
of "chemically" defined media. Cryobiology - Meeting, Kopenhagen, Dänemark
VOSS, J.-U. & SEIBERT, H. (1991) Microcarrier-attached rat hepatocytes as a xenobioticmetabolizing system in cocultures. Cell Biol. Toxicol. 7, 387-399
SEIBERT, H., GÜLDEN, M., KOLOSSA, M. & SCHEPERS, G. (1992) Evaluation of the
relevance of selected in vitro toxicity test system for acute systemic toxicity. ATLA 20, 240-235
VOSS, J.-U. & SEIBERT, H. (1991) Toxicity of glycols and allyl alcohol evaluated by means of
cocultures of microcarrier - attached rat hepatocytes and BALB/c 3T3 cells. ATLA 20, 266-270
EKWALL, B., BARILE, F., BJERREGARD, H., CHESNE, C., CLOTHIER, R., DIERICKX,
P., FISKESJÖ, G., GARCA-OCANAS, L., GOMEZ-LECHON, M.J., GÜLDEN, M.,
KRISTEN, U., KÄRENLAMPI, S., LEWAN, L., NORDIN, M., PERSOONE; G., SEIBERT,
H., SHRIVASTAVA, R., STADTLÄNDER, K., TINGLEFF-SKAANILD, M., TRIGLIA, D.,
TYSON, C., ZUCCO, F. & WALUM, E. (1991) The first ten MEIC chemicals tested in 70
cytotoxicity assays - the influence of cell type and toxicity criteria on cytotoxicity.
IXth Scandinavia Workshop on In Vitro Toxicology, Nagu, Finland
KOLOSSA, M., GÜLDEN, M. & SEIBERT, H. (1991) Einfluß des experimentellen Aufbaus auf
die Ergebnisse von in vitro Zytotoxizitäts - Tests mit proliferierenden Zellen.
In: Schöffl, H., Schulte-Herrmann, R. & Tritthart, H.A. (Eds.) Möglichkeiten und Grenzen der
Reduktion von Tierversuchen; 143-144, Springer-Verlag Wien
PAGE 12
SCHEPERS, G., ASCHMANN, C., GÜLDEN, M. & SEIBERT, H. (1991) Primär kultivierte
Rattenhepatozyten als in vitro Modell zur Erfassung der Wirkung von Chemikalien auf basale
Zellfunktionen und Biotransformationsreaktionen.
In: Schöffl, H., Schulte-Herrmann, R. & Tritthart, H.A. (Eds.) Möglichkeiten und Grenzen der
Reduktion von Tierversuchen; 141-142, Springer-Verlag Wien
VOSS, J.-U. & SEIBERT, H. (1992) Primärkulturen von Rattenhepatozyten auf Microcarriern als
metabolisierendes System in Toxizitätsprüfungen and Zellkulturen. ALTEX 16, 25-37
GÜLDEN, M., KOLOSSA, M. & SEIBERT, H. (1992) Influence of the experimental design on
the outcome of in vitro cytotoxicity tests with proliferating cells. Naunyn-Schmiedeberg's Arch.
Pharmacol. 345, 144
VOSS, J.-U., GÜLDEN, M. & SEIBERT, H. (1992) On the role of hepatocyte cultures in in vitro
toxicity testing. Xth Scandinavian Workshop on In Vitro Toxicology, Visby, Sweden
EKWALL, B., BARILE, F., BJERREGARD, H., CHESNE, C., CLOTHIER, R., DIERICKX,
P., FERRO, M., FISKESJÖ, G., GARCA-OCANAS, L., GOMEZ-LECHON, M.J., GÜLDEN,
M., ISOMAA, B., JACOBSEN, U., KUNIMOTO, M., KRISTEN, U., KÄRENLAMPI, S.,
LEWAN, L., NORDIN, M., PERSOONE, G., SAWYER, T., SEIBERT, H., SHERIVASTAVA,
R., STADTLÄNDER, K., TINGLEFF-SKAANILD, M., TRIGLIA, D., TYSON, C., ZUCCO,
F. & WALUM, E. (1992) Comparison of the results from 100 different cytotoxicity assays of the
first ten MEIC chemicals.
World Congress on Cell & Tissue Culture, Washington, USA
VOSS, J.-U., GÜLDEN, M. & SEIBERT, H. (1992) Cocultures of BALB/c 3T3 cells with
microcarrier-attached rat hepatocytes as an in vitro system for studies on metabolism mediated
cytotoxicity. INVITOX, De Haan, Belgium
EKWALL, B., BARILE, F., CHESNE, C., CLOTHIER, R., CURREN, P., DIERICKX, P.,
EKWALL, O., FERRO, M., FISKESJÖ, G., GARCA-OCANAS, L., GAY, R., GOMEZLECHON, M.J., GÜLDEN, M., HELLBERG, S., IMAI, K., ISOMAA, B., KUNIMOTO,M.,
KRISTEN, U., KÄRENLAMPI, S., LEWAN, L., MALMSTEN, G., PERSOONE, G.,
ROMERT, L., SANDBERG, M., SAWYER, T., SEIBERT, H., SHERVASTAVA, R.,
STAMMATI, A., TANAKA, N., TRIGLIA, D. & WALUM, E. (1992) Further MEIC evaluation:
Acute lethal toxicity in man of reference chemicals nos. 11-30 predicted by 60 in vitro toxicity
assays and by oral LD50 in rodents. INVITOX, De HAAN, Belgium
EKWALL, B., BARILE, F., BONDESSON, I., CLEMEDSON, C., CLOTHIER, R., CURREN,
P., DIERICKX, P., FISKESJÖ, G., GARCA-OCANAS, L., GOMEZ-LECHON, M.J.,
GÜLDEN, M., IMAI, K., JANUS, J., KRISTEN, U., KUNIMOTO, M., KÄRENLAMPI, S.,
LAVRIJSEN, K., LEWAN, L., MALMSTEN, G., MIURA, T., NAKAMURA, M., OHNO, T.,
ONO, H., PERSOONE, G., ROMERT, L., ROUGET, R.L., SANDBERG, M., SAWYER, T.,
SEIBERT, H., SHRIVASTAVA, R., STAMMATI, A., TANAKA, N., WALUM, E., WANG, X.
& ZUCCO, F. (1992) Acute lethal toxicity in man predicted by cytotoxicity in 52 cellular assays
and by oral LD50 tests in rodents for the first 30 MEIC chemicals. 6th Annual Meeting of the
Japanese Society for Alternatives to Animal Experiments, Tokyo, Japan
PAGE 11
GÜLDEN, M., SEIBERT, H., VOSS, J.-U. & WASSERMANN, O. (1992) Animal cells in vitro
as supplement or alternative to animals in acute toxicity testing. Schriftenr. Inst. f. Toxikol. Univ.
Kiel; 21, 1-185
FENTEM, J., FRY, J., GARLE, M., GÜLDEN, M., SEIBERT, H., VOSS, J.-U.,
WASSERMANN, O., PERCHERMEIER, M. & WIEBEL, F. (1993) An International Evaluation
of Selected In Vitro Toxicity Test Systems For Predicting Acute Systemic Toxicity. Report prepared
for the Commission of the European Communities, DGXI (Contract No. B92/B4-3063/14086), 143
pp.
NOACK-FÜLLER, G., DE BEER, C. & SEIBERT, H. (1993) Cadmium, lead, selenium and zinc
in semen of occupationally unexposed men. ANDROLOGIA 25, 7-12
EKWALL, B., CLEMEDSON , C., BARTILE, F., CALLEJA, M.C., CASTELL, J., CHESNE,
C., CLOTHIER, R., CURREN, R., DIERICKX, P., MCFARLANE-ABDULLA, E., FERRO,
M., FISKESJÖ, G., GARZA-OCANAS, L., GÖMEZ-LECHON, M.J., GÜLDEN, M.,
ISOMAA, B., JANUS, J., KERSZMAN, G., KRISTEN, U., KUNIMOTO, M.,
KÄRENLAMPI, S., LAVRIJSEN, K., LEWAN, L., OHNO, T., PERSOONE, G., ROQUET,
R., ROMERT, L., SAWYER, T., SEIBERT, H., SHRIVASTAVA, R., STADTLANDER, K.,
STAMMATI, A., TANAKA, N., TRIGLIA, D., VALENTINO, M., WALUM, E., WANG, X.,
ZUCCO, F., HARVELL, J.D., MAIBACH, H. (1993)
Comparison between human skin irritancy and in vitro cytotoxicity from 77 systems for the first 12
MEIC chemicals. International Symposium "Current trends: In Vitro Skin Toxicology and Eye
Irritancy Testing. April 21-23.
EKWALL, B., CLEMEDSON, C., BARTILE, F., CALLEJA, M.C., CASTEL, J., CHESNE,
C., CLOTHIER, R.,CURREN, R., DIERICKX, P., MCFARLANE-ABDULLA, E., FERRO,
M., FISKESJÖ, G., GARZA-OCANAS, L., GÖMEZ-LECHON, M.J., GÜLDEN, M.,
ISOMAA, B., JANUS, J., KERSZMAN, G., KRISTEN, U., KUNIMOTO, M.,
KÄRENLAMPI, S., LAVRIJSEN, K., LEWAN, L., OHNO, T., PERSOONE, G., ROGUET,
R., ROMERT, L., SAWYER, T., SEIBERT, H., SHRIVASTAVA, R., STADTLANDER, K.,
STAMMATI, A., TANAKA, N., TRIGLIA, D., VALENTINO, M., WALUM, X., WANG, X.,
ZUCCO, F., HARVELL, J.D., MAIBACH, H.(1993)
Human dose-response skin irritancy data for the first 12 MEIC chemicals used to evaluate skin
toxicity prediction by 77 cytotoxicity assays. International Symposium "Current trends: In Vitro
Skin Toxicity and Eye Irritancy Testing" Ottawa, Canada, April 21-23
WALUM, E., MCFARLANE-ABDULLA, E., BARILE, F., BONDESSON, I., CALLEJA,
M.C., CLOTHIER, R., CURREN, R., DIERICKX, P., FISKESJÖ, G., GARZA-OCANAS, L.,
GÖMEZ-LECHON, M.J., GÜLDEN, M., IMAI, K., JANUS, J., KRISTEN, U., KUNIMOTO,
M., KÄRENLAMPI, S., LAVRIJSEN, K., LEWAN, L., OHNO, T., PERSOONE, G.,
ROGUET, R., ROMERT, L., SANDBERG, M., SAWYER, T., SEIBERT, H., SHIVASTAVA,
R., STAMMATI, A., TANAKA, N., ZUCCO, F., CLEMEDSON, C., EKWALL, B. (1993)
Prediction of human acute toxicity by 55 cytotoxicity assays for 30 MEIC chemicals: are the human
systems performing better than systems with animal cells? Submitted to a FRAME conference on
"The use of Human Tissues in In Vitro Toxicology and Testing", April 28-29, Stirling, UK
GÜLDEN, M., SEIBERT, H., & VOSS, J.U. (1994) In vitro toxicity screening using cultured rat
skeletal muscle cells. II. Agents affecting excitable membranes. Toxicology in Vitro 8, 197-206
PAGE 12
GÜLDEN, M., SEIBERT, H., & VOSS, J.U. (1994) Inclusion of physicochemical data in
quantitative comparisons of in vitro and in vivo toxic potencies. ATLA 22, 185-192
GÜLDEN, M., SEIBERT, H. & VOSS, J.U. (1994) The use of cultured skeletal muscle cells in
testing for acute systemic toxicity. Toxicology in vitro 8, 779-782
SEIBERT, H., GÜLDEN, M., & VOSS, J.U. (1994) An in vitro toxicity testing strategy for the
classification and labelling of chemicals according to their potential acute lethal potency.
Toxicology in Vitro 8, 847-850
SEIBERT, H., GÜLDEN, M., & VOSS, J.U. (1994) Comparative cell toxicology: the basis for in
vitro toxicity testing. ATLA 22, 168-174
CLEMEDSON, C., ABDULLA, E., BARILE, F.A., CHESNE, C., CLOTHIER, R., COTTIN,
M., CURREN, R., DIERICKX, P., FERRO, M., FISKESJÖ, G., GARZA-OCANAS, L.,
GOMEZ-LECHON, M.J., GÜLDEN, M., ISOMAA, B., KAHRU, A., KEMP, R.B.,
KERSZMAN, G., KRISTEN, U., KUNIMOTO, M., KÄRENLAMPI, S., LAVRIJSEN, K.,
LEWAN, L., OHNO, T., PERSOONE, G., PETTERSON, R., ROGUET, R., ROMERT, L.,
SAWYER, T., SEIBERT, H., SHRIVASTAVA, M., SJÖSTRÖM, M., TANAKA, N., ZUCCO, F.
& EKWALL, B. (1994) Comparisons of the Toxicity of 30 Chemicals as Measured by 68 Different
in Vitro Toxicity Tests. TCA - meeting, June 4-7.
GÜLDEN, M., SEIBERT, H., VOSS, J. & WASSERMANN, O. (1994) Tierische Zellen in vitro
als Ersatz für Tierversuche: Evaluierung des prognostischen Wertes ausgewählter Testmodelle für
die Abschätzung der systemischen Toxizität von Chemikalien. Abschlußbericht zum BMFT-Projekt
0319312B
SEIBERT, H. (1995) Umweltchemikalien mit östrogener Wirkung - Ein neues Problem? UBATexte 65/95, 11-15
SEIBERT, H. (1996) Umweltchemikalien mit endokriner Wirkung. In : Umweltchemikalien mit
hormoneller Wirkung - Eine Standortbestimmung für Österreich. Tagungsberichte des
Umweltbundesamtes Österreich Bd. 19, 7-21
SEIBERT, H. (1996) Estrogenic chemicals in the environment - a new problem? UBA-Texte 3/96,
10-14
GÜLDEN, M. & SEIBERT, H. (1996) Cytotoxic and non-cytotoxic effects of the MEIC reference
chemicals on spontaneously contracting primary cultured rat skeletal muscle cells. Toxicol. in Vitro
10, 395-406
SEIBERT, H. (1996) Endokrin wirksame Chemikalien in der Umwelt: Störungen der Entwicklung
und Funktion des männlichen Reproduktionssystems. UWSF 8, 275-284
GÜLDEN, M. & SEIBERT, H. (1996) Synergistische Wirkung von Xenoöstrogenen. UWSF 8,
274
CLEMEDSON, C., MCFARLANE-ABDULLA, E., ANDERSSON, M., BARILE, F.A.,
CALLEJA, M.C., CHESNE, C., CLOTHIER, R., COTTIN, M., CURREN, R., DANIELPAGE 11
SZOLGAY, E., DIERICKX, P., FERRO, M., FISKESJÖ, G., GARZA-OCANAS, L.,
GOMEZ-LECHON, M.J., GÜLDEN, M., ISOMAA, B., JANUS, J., JUDGE, P., KAHRU, A.,
KEMP, R.B., KERSZMANN, G., KRISTEN, U., KUNIMOTO, M., KÄRENLAMPI, M.,
LAVRIJSEN, K., LEWAN, L., LILIUS, H., OHNO, T., PERSOONE, G., ROGUET, R.,
ROMERT, R., SAWYER, T.W., SEIBERT, H., SHRIVASTAVA, R., STAMMATI, A.-L.,
TANAKA, N., TORRES-ALANIS, O., VOSS, J.-U., WAKURI, S., WALUM, E., WANG, X.,
ZUCCO, F. & EKWALL, B. (1996) MEIC evaluation of acute systemic toxicity. Part I.
Methodology of 68 in vitro toxicity assays used to test the first 30 reference chemicals. ATLA 24,
Suppl. I, 251-272
CLEMEDSON, C., MCFARLANE-ABDULLA, E., ANDERSSON, M., BARILE, F.A.,
CALLEJA, M.C., CHESNE, C., CLOTHIER, R., COTTIN, M., CURREN, R., DANIELSZOLGAY, E., DIERICKX, P., FERRO, M., FISKESJÖ, G., GARZA-OCANAS, L.,
GOMEZ-LECHON, M.J., GÜLDEN, M., ISOMAA, B., JANUS, J., JUDGE, P., KAHRU, A.,
KEMP, R.B., KERSZMANN, G., KRISTEN, U., KUNIMOTO, M., KÄRENLAMPI, M.,
LAVRIJSEN, K., LEWAN, L., LILIUS, H., OHNO, T., PERSOONE, G., ROGUET, R.,
ROMERT, R., SAWYER, T.W., SEIBERT, H., SHRIVASTAVA, R., STAMMATI, A.-L.,
TANAKA, N., TORRES-ALANIS, O., VOSS, J.-U., WAKURI, S., WALUM, E., WANG, X.,
ZUCCO, F. & EKWALL, B. (1996) MEIC evaluation of acute systemic toxicity. Part II. In vitro
results from 68 toxicity assays used to test the first 30 reference chemicals and a comparative
cytotoxicity analysis. ATLA 24, Suppl. I, 273-311
SEIBERT, H., BALLS, M., FENTEM, J. H., BIANCHI, V., CLOTHIER, H., DIERICKX, P.J.,
EKWALL, B., GARLE, M.J., GOMEZ-LECHON, M.J., GRIBALDO, L., GÜLDEN, M.,
LIEBSCH, M., RASMUSSEN, E., ROGUET, R., SHRIVASTAVA, R., & WALUM E. (1996)
Acute Toxicity Testing In Vitro and the Classification and Labelling of Chemicals. The report and
recommendations of ECVAM Workshop 16. ATLA 24, 499 - 510
SEIBERT, H. (1997) Beeinträchtigung der männlichen Fertilität durch endokrin wirksame Stoffe.
In: Niedersächsisches Umweltministerium (Hrsg.) Schadstoffe mit hormoneller Wirkung - Wie
sicher ist unsere Zukunft? Internationales Hearing, Hannover, Tagungsband, 69-73
SEIBERT, H. (1997) Chemikalien mit endokriner Wirkung - Forschungsstand und
Forschungsbedarf. Journalistenseminar der Information Umwelt, Berlin; Hrsg.: GSFForschungszentrum für Umwelt und Gesundheit, Band 21, 56-59
GÜLDEN, M. & SEIBERT, H. (1997) Influence of protein binding and lipophilicity on the
distribution of chemical compounds in in vitro systems. Toxicology in Vitro 11, 479-483
GÜLDEN, M., TURAN, A. & SEIBERT, H. (1997) Substanzen mit endokriner Wirkung in
Oberflächengewässern. Hrsg.: Umweltbundesamt Berlin, UBA-Texte 46/97, 362 pp
GÜLDEN, M., SEIBERT, H. & VOSS, J. (1997) Toxikologische Bewertungen ausgewählter
organischer Stoffe für die Klassierung nach Nr. 3.1.7 TA Luft. Schriftenreihe des Instituts für
Toxikologie der Universität Kiel, Heft Nr. 36, 163 S.
GÜLDEN, M. & SEIBERT, H. (1997) Nicht reproduzierbar: Versuchsergebnisse zur
synergistischen Wirkung von Xenoöstrogenen. UWSF 9, 211-212
PAGE 12
SEIBERT, H. (1998) Pestizide und Industriechemikalien mit unerwünschter hormoneller Wirkung
und ihr Vorkommen in Oberflächengewässern. In: Hormonal wirksame Substanzen in Umwelt und
Lebensmitteln: Vorkommen, Wirkungen und toxikologische Bewertung. 8. Seminar für
Toxikologie, Graz, Proceedings, 43-47
BRUHN, T., LUDEWIG, S., GÜLDEN, M. & SEIBERT, H. (1999) Einstufung von Schadstoffen
als endokrin wirksame Substanzen. Hrsg.: Umweltbundesamt Berlin, UBA-Texte 65/99, 143 S.
SEIBERT, H. (1998) Substanzen mit endokriner Wirkung in Oberflächengewässern. In:
Chemikalien in der Umwelt mit Wirkung auf das endokrine System. Hrsg.: GSFForschungszentrum Neuherberg, 22-24
SEIBERT, H. (1998) Einstufung von Schadstoffen als endokrin wirksame Substanzen. In:
Chemikalien in der Umwelt mit Wirkung auf das endokrine System. Hrsg.: GSFForschungszentrum Neuherberg, 25-26
GÜLDEN, M., TURAN, A. & SEIBERT, H. (1998) Endocrinically active chemicals and their
occurrence in surface waters. Hrsg.: Umweltbundesamt Berlin, UBA-Texte 66/98, 362 S.
GÜLDEN, M., TURAN, A. & SEIBERT, H. (1999) Substanzen mit endokriner Wirkung in
Oberflächengewässern. ATV-Schriftenreihe 15, 38-50
GÜLDEN, M., MÖRCHEL, S. & SEIBERT, H. (2000) Factors influencing nominal effective
concentrations of chemical compounds in vitro: cell concentration. Naunyn-Schmiedeberg’s Arch.
Pharmacol. Suppl. 361, R 164
RÖHL, C., GÜLDEN, M. & SEIBERT, H. (2000) Effects of trialkyltin compounds on rat
astrocytes in primary culture. Naunyn-Schmiedeberg’s Arch. Pharmacol. Suppl. 361, R 143
GÜLDEN, M., MÖRCHEL, S. & SEIBERT, H. (2001) Factors influencing nominal effective
concentrations of chemical compounds in vitro: cell concentration. Toxicol. in Vitro 15, 233-234
RÖHL, C., GÜLDEN, M. & SEIBERT, H. (2001) Toxicity of organotin compounds in primary
cultures of rat cortical astrocytes. Cell Biol. Toxicol. 17, 23-32
MURIAS, M., MATLAWSKA, I., BYLKA, W., GÜLDEN, M. & SEIBERT, H. (2001) An in
vitro investigation of antioxidant properties of Aquilegia vulgaris preparations using cultured C6
glioma cells. 6. Conference „Interactions of Xenobiotics“, Kiekrz, Polen
SEIBERT, H., MÖRCHEL, S. & GÜLDEN, M. (2002) Factors influencing nominal effective
concentrations of chemical compounds in vitro: medium protein concentration. Toxicol. in Vitro 16,
289-297
GÜLDEN, M., MÖRCHEL, S., TAHAN, S. & SEIBERT, H. (2002) Impact of protein binding on
the availability and cytotoxic potency of organochlorine pesticides and chlorophenols in vitro.
Toxicology 175, 201-213
SEIBERT, H. (2002) Wirkmechanismen von Umweltchemikalien mit endokrinem Potenzial. In:
BfG (Hrsg.) Neuartige toxische Effekte und ihre Relevanz für die Baggergutbeurteilung. BfGVeranstaltungen 2/2002
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ZIELINSKA, M:, GÜLDEN, M. & SEIBERT, H. (2003) Effects of quercetin and quercetin-3-Oglycosides on oxidative damage in rat C6 glioma cells. Environm. Toxicol. Pharmacol. 13, 47-53
GÜLDEN, M., MÖRCHEL, S., SEIBERT, H. (2003) Serum albumin binding at cytotoxic
concentrations of chemicals as determined with a cell proliferation assay. Toxicology Letters 137,
159-168
GÜLDEN, M., SEIBERT, H. (2003) Estimation of human serum concentrations of chemicals
equivalent to toxic concentrations in vitro. Naunyn-Schmiedeberg’s Arch. Pharmacol. 367 (Suppl.
1) R 162
GÜLDEN, M., SEIBERT, H. (2003) In vitro-in vivo extrapolation: estimation of human serum
concentrations of chemicals equivalent to cytotoxic concentrations in vitro. Toxicology 189,
211-222
GÜLDEN, M., SEIBERT, H. (2003) Bioverfügbarkeit von Chemikalien in vitro und ihre
Bedeutung für toxische Potenzen und In vitro-in vivo-Extrapolationen. SETAC Tagung Heidelberg
GÜLDEN, M. & SEIBERT, H. (2004) Bioavailability of chemicals in vitro: impact on predictions
of toxic potencies in mammals and aquatic test organisms. Naunyn-Schmiedeberg’s Arch.
Pharmacol. 369 (Suppl. 1) R 556
SEIBERT, H., MÖRCHEL, S., GÜLDEN, M. (2004) Cytotoxic potency of trialkyltins to C6
glioma cells in vitro: impact of exposure conditions. Cell Biology and Toxicology 20, 273-283
WULF, B., GÜLDEN, M., SEIBERT, H. (2004) Further evaluation of the MEIC in vitro toxicity
data base. INVITOX 2004
GÜLDEN, M. SEIBERT, H. (2004) Quantitative in vitro-in vivo extrapolation of cytotoxic
potencies determined in vitro. INVITOX 2004
GÜLDEN, M., SEIBERT, H. In vitro testing for acute systemic toxicity assessment: integration of
biokinetic parameters. INVITOX 2004
GÜLDEN, M., MÖRCHEL, S. SEIBERT, H. (2005) Comparison of mammalian and fish cell line
cytotoxicity: impact of endpoint and exposure duration. Aquatic Toxicology 71, 229-236
GÜLDEN, M., SEIBERT, H. (2005) Impact of bioavailability on the correlation between in vitro
cytotoxic and in vivo acute fish toxic concentrations of chemicals. Aquatic Toxicology 72, 327-337
SEIBERT, H. (2005) In vitro-Tests zur Prüfung potenziell endokriner Wirkungen von chemischen
Verbindungen: die Arbeit der „Validation Management Group – Non-animal Assays“ bei der
OECD.
3. Statusseminar: Chemikalien in der Umwelt mit Wirkung auf das endokrine System, Berlin.
Proceedings: 94-96
SEIBERT, H., GÜLDEN, M. (2005) Assessment of the systemic toxicity of chemicals by means of
in vitro assays: toxicodynamic and toxicokinetic aspects. ETS & SSCT joint conference, Toila,
Estland
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SEIBERT, H., WULF, B. GÜLDEN, M. (2005) Comparative toxicity of chemicals in vitro:
further evaluation of the data of the MEIC programme. ETS & SSCT joint conference, Toila,
Estland
GÜLDEN, M., SEIBERT, H. (2005) Improvement of in vitro cytotoxicity testing for assessing
acute fish toxicity. ATLA (in press)
KUZIW, M., GÜLDEN, M., MASER, E., SEIBERT, H. (2005) Protection of C6 glioma cells by
polyphenols against peroxide-induced oxidative stress. ETS & SSCT joint conference, Toila,
Estland
MÜLLER, A., GÜLDEN, M., MASER, E., SEIBERT, H. (2005) Influence of pre-loading on the
cytoprotective activity of F6 01-tocopherol against oxidative stress in C6 glioma cells. ETS & SSCT
joint conference, Toila, Estland
GÜLDEN, M., SEIBERT, H. (2006) In vitro-in vivo extrapolation of toxic potencies for hazard
and risk assessment – problems and new developments. 5 th World Congress on Alternatives &
Animal Use in the Life Sciences, Berlin; Proceedings, ALTEX 23: 218-225
GÜLDEN, M., DIERICKX, P., SEIBERT, H. (2006) Validation of a prediction model for
estimating serum concentrations of chemicals which are equivalent to toxic concentrations in vitro.
Toxicology in Vitro 20, 1114-1124
SEIBERT, H., GÜLDEN, M. (2006) Impact of experimental conditions on toxic activities of
chemicals in vitro – Part I: Experimental studies. 24th Workshop of the Scandinavian Society of Cell
Toxicology; Grinda, Sweden
GÜLDEN; M., SEIBERT, H. (2006) Impact of experimental conditions on toxic activities of
chemicals in vitro – Part II: modelling of distribution and bioavailability. 24th Workshop of the
Scandinavian Society of Cell Toxicology; Grinda, Sweden
RÜWELER, M., GÜLDEN, M., MASER, E., MURIAS; M., SEIBERT, H. (2006) Cytotoxicity
of resveratrol and analogues and cytoprotection against oxidative stress in C6 astroglioma cells in
vitro. 24th Workshop of the Scandinavian Society of Cell Toxicology, Grinda, Sweden
BEKEN, J., JIROVA, D., PÜSSA, T., LARUE, L., LIEBSCH, M., STAMMATI, A., VAN DER
VALK, J., MIRCIOIU, C., BREIER, A., CASTANO, A., AMCOFF, P., RICHMOND, J.,
WESTMORELAND, C., ROGIERS, V., ALEPEE, N., COMBES, R., SEIBERT, H. (2007)
ESAC Statement on the validity of in vitro tests for skin irritation. ATLA 35, 308-312
BEKEN, J., JIROVA, D., PÜSSA, T., LARUE, L., LIEBSCH, M., STAMMATI, A., VAN DER
VALK, J., MIRCIOIU, C., BREIER, A., CASTANO, A., AMCOFF, P., RICHMOND, J.,
WESTMORELAND, C., ROGIERS, V., ALEPEE, N., COMBES, R., SEIBERT, H. (2007)
ESAC Statement on the Reduced Local Lymph Node Assay (rLLNA). ATLA 35, 306-307
BEKEN, J., JIROVA, D., PÜSSA, T., LARUE, L., LIEBSCH, M., STAMMATI, A., VAN DER
VALK, J., MIRCIOIU, C., BREIER, A., CASTANO, A., AMCOFF, P., RICHMOND, J.,
WESTMORELAND, C., ROGIERS, V., ALEPEE, N., COMBES, R., SEIBERT, H. (2007)
ESAC Statement on the conclusions of the ICCVAM retrospective study on organotypic in vitro
assays as screening tests to identify potential ocular corrosives and severe irritants as determined by
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US EPA, EU(R41) and UN GHS classifications in a tiered testing strategy, as part of a weight of
evidence approach. http://ecvam.jrc.it/
SEIBERT, H., HORBACH, S., BLAAUBOER, B. (2007) Fourteenth international workshop on
in vitro toxicology, 2-5 October 2006, Toxicology in Vitro21, 1213 – 1214
GÜLDEN, M., SEIBERT, H. (2007) The improvement of in vitro cytotoxicity testing for the
assessment of acute toxicity in fish. ATLA 35, 39-46
BEKEN, S., BREIER, A., DAMBROVA, M., HORVATH, K., JIROVA, D., KOLAR, R.,
KNUDSEN, E., LIEBSCH, M., LARUE, L., DAL NEGRO, G., NIKOLAIDIS, E.,
MIRCIOIU, C., PFALLER, W., RICHMOND, J., ROGIERS, V., SEIBERT, H.,
SLADOWSKI, D., VAN DER VALK, J., WESTMORELAND, C., YLIKOMI, T. (2008)
ESAC Statement on the performance Standards (PSs) for the Local Lymph Node Assay (LLNA).
http://ecvam.jrc.it
LINDEN, A., GÜLDEN, M., MARTIN, H-J., MASER, E., SEIBERT, H. (2008) Peroxideinduced cell death and lipid peroxidation in C6 glioma cells. Toxicology in Vitro 22, 1371-1376
RÜWELER, M., ANKER, A., GÜLDEN, M., MASER, E. SEIBERT, H. (2008) Inhibition of
peroxide-induced radical generation by plant polyphenols in C6 astroglioma cells. Toxicology in
Vitro 22, 1377-1381
PFALLER, W., JIROVA, D., KNUDSEN, E., PÜSSA, T., YLIKOMI, T., LIEBSCH, M.,
NIKOLAIDIS, E., HORVATH, K., STAMMATI, A., DAMBROVA, M., MAURICA, M., VAN
DER VALK, J., MIRCIOIU, C., CASTANO, A., AMCOFF, P., WESTMORELAND, C.,
ROGIERS, V., DAL NEGRO, G., SEIBERT, H., JENNINGS, M., KOLAR, R. (2008) ESAC
Statement on the use of FCS and other animal-derived supplements. ATLA 36, 481-482
SEIBERT, H. (2008) 25th Workshop of the Scandinavian Society for Cell Toxicology, Salzau,
Germany, 2007. Toxicology in Vitro 22,1349
RÜWELER, M., GÜLDEN, M., MASER, E., MURIAS, M., SEIBERT, H. (2009) Cytotoxic,
cytoprotective and antioxidant activities of resveratrol and analogues in C6 astroglioma cells in
vitro. Chemico-Biological Interactions 182, 128-135
BRENNER, S., GÜLDEN, M., MASER, E., SEIBERT, H. (2010) Lasting effect of preceding
culture conditions on the susceptability of C6 cells to peroxide-induced oxidative stress. Toxicology
in Vitro 24, 2090-2096
CASTANO, A., DAMBROVA, M., GRAY, A., HORVATH, K., JENNINGS, M., JIROVA, D.,
KOLAR, R., KNUDSEN, E., LIEBSCH, M., DAL NEGRO, G., PFALLER, W., PÜSSA, T.,
ROGIERS, V., SEIBERT, H., STAMMATI, A., VAN DER VALK, J., WESTMORELAND, C.,
(2009) ESAC Statement on the scientific validity of an in vitro test method for skin corrosivity
testing. http://ecvam.jrc.it/
CASTANO, A., DAMBROVA, M., GRAY, A., HORVATH, K., JENNINGS, M., JIROVA, D.,
KOLAR, R., KNUDSEN, E., LIEBSCH, M., DAL NEGRO, G., PFALLER, W., PÜSSA, T.,
ROGIERS, V., SEIBERT, H., STAMMATI, A., VAN DER VALK, J., WESTMORELAND, C.,
(2009) Statement on the performance under GHS of three in vitro assays for skin irritation testing
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and the adaptation of the reference chemicals and defined accuracy values of the ECVAM skin
irritation performance standards. http://ecvam.jrc.it/
GÜLDEN, M., JESS, A., KAMMANN, J., MASER, E., SEIBERT, H. (2010) Cytotoxic potency
of H2O2 in cell cultures: impact of cell concentration and exposure time. Free Radical Biology &
Medicine 49, 1298-1305
GÜLDEN, M., SEIBERT, H. (2010) Concentration or dose: what is the proper measure for
quantitative in vitro-in vivo extrapolation of toxic potency? ALTEX 27, 46-47
KAMMANN, J., GÜLDEN, M., MASER, E., SEIBERT, H (2010) Variability of elimination rate
and cytotoxic potency of peroxides in C6 astroglioma cell cultures. ALTEX 27, 65-66
SEIBERT, H., JESS, A., LINDEN, A., MASER, E., PERLE, M., GÜLDEN, M. (2010)
Peroxide-induced oxidative stress and cell death in C6 astroglioma cells. ALTEX 27, 123
SEIBERT, H., MASER, E., SCHWEDA, K., SEIBERT, S., GÜLDEN, M. (2011) Cytoprotective activity against peroxide-induced oxidative damage and cytotoxicity of flavonoids in C6 rat
glioma cells. Food and Chemical Toxicology 49, 2398 - 2407
SEIBERT, H. & GÜLDEN, M. (2011) In vitro-in vivo extrapolation of toxic potencies: basic
requirements. 28th Workshop of the Scandinavian Society for Cell Toxicology, Tampere, Finland,
Abstracts, 27
KAMMANN, J., GÜLDEN, M., SEIBERT, H. (2012) Erstellung eines Konzeptes zur integrierten
Bewertung der Umwelt- und Humandaten der Umweltprobenbank des Bundes. Abschlußbericht
zum UBA-Projekt: 301 02 046, Band 1 und 2 (569 Seiten)
BlaauboeR, B., J., Boekelheide, K., Clewell, H., J., Daneshian, M., Dingemans, M., M., L.,
Goldberg, A., M., Heneweer, M., Jaworska, J., Kramer, N., I., Leist, M., Seibert, H., Testai, E.,
VandebrieL, R., J., Yager, J. D. and Zurlo, J. (2012) The use of biomarkers of toxicity for
integrating in vitro hazard estimates into risk assessment for humans. Transatlantic think tank for
toxicology, t4 Workshop Report, ALTEX 29, 411- 425
Klauser, E., Gülden, M., Maser, E., Seibert, S., Seibert, H. (2013) Effects of
genistein and buthionine sulfoximine on arsenic trioxide-induced growth inhibition in C6
astroglioma cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 386, S41
SCHÜLER, W., GÜLDEN, M., MASER, E., SEIBERT, S., SEIBERT, H. (2013) Direct and
sustained cytoprotective activity of quercetin in C6 astroglioma cells exposed to continuously
generated or bolus applied H2O2. Naunyn-Schmiedeberg's Arch. Pharmacol. 386, S75
GÜLDEN, M., SCHREINER, J., SEIBERT, H. (2013) In vitro toxicity testing with microplate
cell cultures: impact of cell binding. Toxicology, doi: 10.1016/j.tox.2013.11.006
KLAUSER, E., GÜLDEN, M., MASER, E., SEIBERT, S., SEIBERT, H. (2014) Additivity,
antagonism, and synergy in arsenic trioxide-induced growth inhibition of C6 glioma cells: effects of
genistein, quercetin and buthionine-sulfoximine. Food and Chemical Toxicology 67, 212-221
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