Immunisation with foamy virus Bet fusion proteins as novel strategy

Immunisation with foamy virus Bet fusion proteins as novel
strategy for HIV-1 epitope delivery. Immunologic Research,
M. Mühle, K. Hoffmann, M. Löchelt, J. Denner
Online Resource 2
2F5/Bet-E1
Response [RU]
250
200
150
25
100
0
-200 0
Response [RU]
200 400 600 800 1000 1200 1400
2F5/Bet-E2
250
50
200
150
100
25
12.5
50
KD = 4.3 nM
0
-50
-200 0
200 400 600 800 1000 1200 1400
2F5/Bet-E1+Bet-E2
200
Response [RU]
100
50
-50
150
50
100
25
12.5
50
0
-50
KD = 5.9 nM
-200 0
200 400 600 800 1000 1200 1400
2F5/Bet-E1-E2
Response [RU]
250
200
150
100
50
0
-50
-100
25
250
200
150
100
50
0
-50
-100
-150
-200
100
50
KD = 19 nM
-500
Response [RU]
50
Surface plasmon resonance experiments
with the antigens used for immunisation
and the antibodies 2F5 and 4E10. The
signal intensity in response units (RU), the
protein concentrations (in nM), protein and
buffer injection points (black and grey
arrows) and calculated KD values are
indicated for interactions measured with
captured 2F5 antibody and Bet-E1, Bet-E2,
Bet-E1+ Bet-E2 and Bet-E1-E2 or for
interaction measured with captured 4E10
antibody and the Bet-E1-E2 antigen.
Response curves are shown as grey lines,
fitted curves are in black. Note that
response peaks between individual
injections (arrows) correspond to bulk
contributions as a result of differences in
the buffer composition
0
500
1000
1500
2000
4E10/Bet-E1-E2
100
200
400
KD = 224 nM
0
200 400 600 800 1000 1200 1400
Time [s]
correspondence to:
[email protected], Robert Koch Institute, Center for HIV and Retrovirology, Berlin, Nordufer 20, 13553 Germany