Rabbit Polyclonal Antibody to Human Cadherin

Rabbit Polyclonal Antibody to Human
Cadherin-1 / CDH1 / CD324 ( Antigen Affinity Purified )
Catalog Number:
10204-RP02
Preparation
General Information
Immunogen:
Recombinant human CDH1 protein
( Catalog#10204-H08H )
Produced in rabbits immunized with purified, human cell-derived, recombinant human
Cadherin-1 / CDH1 (rhCadherin-1; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707).
Cadherin-1 specific IgG was purified by human Cadherin-1 affinity chromatography.
Ig Type:
Rabbit IgG
Storage
Applications:
WB, ELISA, IHC-P, IHC-Fr
Specificity:
Human Cadherin-1 / CDH1 / CD324
Formulation:
0.2 μm filtered solution in PBS with
5% trehalose
Storage:
﹤ -20° C
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity.
Antibody products are stable for twelve months from date of receipt when stored at -20℃ to
-80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It
is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications
Immunochemistry –
IHC-P: 0.5-2 μg/mL
IHC-Fr: 5-15 μg/mL
Immunochemical Staining of human E-cadherin in
human gastric cancer with Rabbit Polyclonal Antibody (1
µg/mL, formalin-fixed paraffin embedded sections).
Positive staining was localized to membrane of epithelial
cells.
10204-RP02
Antigen Blocking
skin
liver
+
－
+
ND
colon
carcinoma
+
ND
gastric
cancer
+
ND
Immunochemical Staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody (1 µg/mL, formalinfixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
positive： +
negtive:
－
No Data: ND
Immunofluorescence staining of human E-cadherin in
human skin with Rabbit Polyclonal Antibody (5 µg/mL,
frozen sections). An DyLight488 goat anti-rabbit
antibody was used as the secondary antibody. The
image showing membrane staining of squamous
epithelium cells.
Immunochemical Staining of human E-cadherin in liver
with Rabbit Polyclonal Antibody (1 µg/mL, formalin-fixed
paraffin embedded sections). Positive staining was
localized to membrane of hepatocytes.
Immunochemical Staining of human E-cadherin in
human colon carcinoma with Rabbit Polyclonal Antibody
(1 µg/mL, formalin-fixed paraffin embedded sections).
Positive staining was localized to membrane of epithelial
cells.
Manufactured By Sino Biological Inc., FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Fax :+86-10-51029969
Tel:+86-400-890-9989
http://www.sinobiological.com
Rabbit Polyclonal Antibody to Human
Cadherin-1 / CDH1 / CD324 ( Antigen Affinity Purified )
Catalog Number:
10204-RP02
Western blot –
WB: 10-20 μg/mL
Lanes
A
Items
Sample
(whole cell lysate )
HepG2
Sample Volume
(μ g/lane)
30
Gel
Recommended
Concentration
Secondary Antibody
13% SDS-PAGE reducing gel
10-20 μ g/ml
Dylight 800-labeled Antibody To Rabbit IgG (H+L), at
1:5000 dilution.
Direct ELISA – This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary
reagents to detect Cadherin-1. The detection limit for human Cadherin-1 is 0.00245ng/well.
Specificity
Human Cadherin-1 / CDH1 / CD324
Cross reactivity
Does not react with
Rat
Background
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with
themselves in a homophilic manner in connecting cells, and thus may contribute to the
sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is
the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the
extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfidelinked homodimer, and plays a key role in the organization and integrity of most epithelial
tissues, cell differentiation and tissue development. CDH1 interacts directly, via the
cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion
complex which connects to the actin skeleton through the actin binding of alpha-catenin.
During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to
produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (ECAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as
downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion
system, and often correlates with strong invasive potential and poor prognosis of human
carcinomas (gastric, breast, ovary, endometrium and thyroid).
Reference
1. Berx G. et al., 1995, Genomics 26: 281-289.
2. Yoshiura K. et al., 1995, Proc. Natl. Acad. Sci. USA. 92: 7416-7419.
3. Becker KF. et al., 1994, Cancer Res. 54: 3845-3852.
4. Marambaud P. et al., 2002, EMBO J. 21: 1948-1956.
5. Steinhusen U. et al., 2001, J. Biol. Chem. 276: 4972-4980.
Manufactured By Sino Biological Inc., FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Fax :+86-10-51029969
Tel:+86-400-890-9989
http://www.sinobiological.com